A photoprotective whitening and repairing composition suitable for sensitive skin and its application

Abstract

This invention relates to a photoprotective, whitening, and repairing composition suitable for sensitive skin and its application. The composition comprises the following components: oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and Lactobacillus fermentation product, wherein the weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and Lactobacillus fermentation product is (0.01-6):(0.01-0.5):(0.01-2):(0.1-10). This invention, through the combination of multi-target components, overcomes the efficacy limitations of traditional photoprotective, whitening, and repairing products. This invention provides a highly efficient and safe solution for photoprotection, repair, and whitening of sensitive skin, possessing significant application value and market potential.

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a photoprotective, whitening, and repairing composition suitable for sensitive skin and its application. Background Technology

[0002] With increasing environmental light pollution, the damage to the skin caused by ultraviolet radiation in the air is becoming more and more significant, leading to a series of problems such as skin sensitivity, inflammation, dullness, and aging. Therefore, developing cosmetics with photoprotective, repairing, and whitening effects has become a research hotspot in the industry.

[0003] Existing photoprotection and whitening repair technologies mostly focus on single ingredients or traditional carriers. Single ingredients cannot fully resist multi-dimensional photopollutation damage and lack comprehensive regulation of inflammation repair, barrier reconstruction and whitening and brightening. Traditional emulsions or microcapsule carriers have problems such as excessively large particle size (>500nm), poor stability and low bioavailability, which makes active ingredients easy to degrade and difficult to penetrate into the deep layers of the skin.

[0004] Oxidized resveratrol, as a natural polyphenol compound, possesses various biological activities such as anti-photooxidation, anti-inflammation, and whitening, and has broad application prospects in the cosmetics field. However, oxidized resveratrol suffers from poor water solubility, low stability, and low bioavailability, which limits its application efficacy in cosmetics. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a photoprotective whitening and repairing composition suitable for sensitive skin and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] In a first aspect, the present invention provides a photoprotective whitening and repairing composition suitable for sensitive skin, comprising the following components: oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product, wherein the weight ratio of oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product is (0.01-6):(0.01-0.5):(0.01-2):(0.1-10);

[0008] The preparation method of the oxidized resveratrol nanocrystals includes the following steps:

[0009] (1) Mix resveratrol oxide and phosphatidylcholine in anhydrous ethanol and stir until they are evenly dissolved to obtain the solvent phase; wherein, the ratio of resveratrol oxide, phosphatidylcholine and anhydrous ethanol is 1g:(0.1-0.3)g:(8-12)mL.

[0010] (2) Dissolve hydroxypropyl methylcellulose in water, heat to 35-45℃ and degas by ultrasonication to obtain an antisolvent phase; obtain a hydroxypropyl methylcellulose solution; wherein the ratio of hydroxypropyl methylcellulose to water is 1g:(80-100)mL;

[0011] (3) Add the solvent phase dropwise to the antisolvent phase while stirring. After the addition is complete, continue stirring until precipitation is complete to obtain a suspension.

[0012] (4) After homogenizing the suspension, centrifuge, discard the supernatant and resuspend in distilled water, and repeat centrifugation-resuspending to obtain the purified nanocrystal suspension. Then freeze-dry to obtain the oxidized resveratrol nanocrystals.

[0013] The method for preparing the Lactobacillus fermentation product includes the following steps:

[0014] S1. Activation and Expansion Culture of the Strain: Lyophilized Lactobacillus powder was inoculated into MRS medium for culture to obtain a seed culture; the viable count in the seed culture was 1 × 10⁻⁶. 8 -2×10 9 CFU / mL;

[0015] S2. Mix sodium alginate with distilled water evenly and sterilize to obtain sodium alginate solution; wherein, the ratio of sodium alginate to distilled water is 1g:(20-30)mL;

[0016] S3. Stir the sodium alginate solution and the seed liquid evenly to obtain a mixed solution; wherein the volume ratio of sodium alginate solution to seed liquid is (2-5):1;

[0017] S4. Using a sterile syringe, drop the mixture into a pre-cooled calcium chloride solution to form spherical gel beads with a diameter of 2-3 mm. Allow the gel beads to stand in the calcium chloride solution to harden. After filtration, wash, centrifuge, discard the supernatant, and dry to obtain immobilized gel beads. The temperature of the calcium chloride solution is 4℃, the standing hardening time is 30-60 min, the volume ratio of the mixture to the calcium chloride solution is 1:(6-8), and the mass concentration of the calcium chloride solution is 1-3%. The centrifugation speed is 3000-5000 r / min for 3-5 min, and the drying temperature is 70-75℃ for 3-5 min.

[0018] S5. Add the immobilized gel beads to the fermentation medium for anaerobic fermentation. Terminate the fermentation when pH ≤ 4.0 and lactic acid production no longer increases, to obtain a fermentation mixture. The ratio of immobilized gel beads to fermentation medium is 1 g: (9-11) mL. Preferably, the anaerobic fermentation temperature is 30-37℃.

[0019] S6. The fermentation mixture is subjected to solid-liquid separation to obtain a supernatant. The supernatant is then concentrated by ultrafiltration, decolorized, and filtered to obtain the lactobacillus fermentation product.

[0020] Oxidized resveratrol, a derivative of resveratrol, boasts higher bioavailability. Its antioxidant capacity is 800 times higher than vitamin C, rapidly neutralizing light-polluting-induced oxygen free radicals (such as ROS) and nitrogen free radicals, blocking lipid peroxidation chain reactions, and reducing skin oxidative damage at its source. Combined with enzymatically hydrolyzed gardenia extract, the enzymatic hydrolysis technology enhances the permeability of active ingredients, precisely inhibiting the NF-κB inflammatory pathway, quickly relieving PM2.5-induced redness and sensitivity, and simultaneously chelating heavy metal ions (such as cadmium and lead) to block oxidative stress chain reactions. Oat β-glucan forms a uniform and dense physical barrier film, enhancing skin's water-locking ability and activating Langerhans cells to repair the damaged barrier, constructing a dual "protection-repair" mechanism with gardenia extract. Immobilized cell fermentation technology can improve the purity and activity of fermentation products while reducing production costs. Furthermore, the lactobacillus fermentation products are rich in short-chain fatty acids, which can regulate the skin's microecological balance and inhibit the proliferation of related acne-causing bacteria. Its weakly acidic environment (pH...) 4.5-5.5) Further optimize the dispersion stability of nanocrystals to ensure the long-lasting activity of the composition. Through multi-pathway synergistic repair (anti-photooxidation-anti-inflammation-barrier reconstruction-microecological regulation) and enhanced component interaction (gardenia acid promotes β-glucan film formation, and the pH of fermentation products maintains nanocrystal stability), the four components form a comprehensive protection system against urban light pollution, providing an efficient, stable, and low-burden innovative solution for the field of anti-light pollution skincare.

[0021] In this invention, nanocrystal technology is used to co-grind oxidized resveratrol with phosphatidylcholine (a surface modifier) ​​and hydroxypropyl methylcellulose (as a stabilizer) to form a 200nm nanocrystal structure, significantly enhancing its photoprotective, whitening, and repairing efficacy. Hydroxypropyl methylcellulose forms a three-dimensional network hydration layer on the crystal surface, effectively blocking air- and light-induced oxidative degradation. Furthermore, the thickening effect of hydroxypropyl methylcellulose enhances the film-forming synergy between the nanocrystals and oat β-glucan (a patented composition component), forming a dense protective network on the skin surface, reducing pollutant adhesion. Simultaneously, the moisturizing properties of phospholipids enhance the skin's water-locking ability. Ultimately, through a "nanocrystal synergy-multi-component synergy" mechanism, anti-photooxidation, whitening, and... The invention offers a triple benefit in repairing the skin barrier. The nanocrystal technology significantly improves the solubility, utilization, and stability of oxidized resveratrol: phosphatidylcholine reduces the surface tension of oxidized resveratrol through bilayer encapsulation, significantly enhancing its solubility and utilization. It also adsorbs onto the nanocrystal surface through hydrophobic interactions to prevent aggregation. Simultaneously, hydroxypropyl methylcellulose acts as a steric hindrance stabilizer, further improving the stability of the nanocrystals. The nanocrystal technology also significantly improves the permeability of oxidized resveratrol: the small particle size of the nanocrystals allows them to penetrate the stratum corneum interstitial space, achieving targeted delivery by combining with the cell membrane-like structure of phospholipids. This results in a significantly higher transdermal absorption rate compared to traditional formulations, enabling oxidized resveratrol to more effectively exert its whitening and antioxidant effects.

[0022] Preferably, the weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product is (0.1-3):(0.05-0.3):(0.1-1):(1-5).

[0023] More preferably, the weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product is (0.5-1.2):(0.1-0.2):(0.4-0.5):(2-3).

[0024] Specifically, the preparation method of the gardenia extract includes the following steps:

[0025] (1) Raw material pretreatment: Gardenia flowers are dried to a moisture content of ≤10%, pulverized and sieved to obtain gardenia flower powder;

[0026] (2) Compound enzymatic hydrolysis: Gardenia powder is mixed with compound enzyme solution and subjected to ultrasonic enzymatic hydrolysis. After enzymatic hydrolysis, the enzyme is inactivated by heating to obtain a mixed solution. The solvent of the compound enzyme solution is distilled water. The compound enzyme solution includes a compound enzyme of cellulase and pectinase in a mass ratio of 1:(0.8-1.1). The mass concentration of the compound enzyme in the compound enzyme solution is 0.5-1%. The material-liquid ratio of gardenia powder to compound enzyme solution is 1g:(15-25)mL. Cellulase is used to degrade cell wall cellulose, and pectinase is used to decompose intercellular pectin.

[0027] (3) Extraction: The mixture is heated at 75-85℃ to extract the extract.

[0028] (4) Solid-liquid separation and purification: Centrifuge the extract, collect the supernatant, filter, and obtain the extract;

[0029] (5) Concentration and drying: The extract is concentrated and dried to obtain the gardenia extract in powder form.

[0030] Preferably, in step (1) of preparing the gardenia extract, before drying, fresh or dried gardenia flowers are selected, and impurities and non-medicinal parts (such as flower stalks) are removed; after pulverization, they are passed through a 40-60 mesh sieve.

[0031] Preferably, in step (2) of the preparation of the gardenia extract, the pH of the enzymatic hydrolysis is 4.5-5.0, and the pH is adjusted using a citrate-sodium citrate buffer solution with a molar concentration of 0.05-0.2 mol / L; the parameters for ultrasonic enzymatic hydrolysis are: frequency 35-45 kHz, power 250-350 W, and time 25-35 minutes. The cavitation effect of ultrasound (generating and collapsing microbubbles, releasing high temperature and pressure) and the mechanical effect (accelerating the interaction between the solvent and the cell) work together to destroy the cell wall, allowing the enzyme to fully contact the intracellular substances. The parameters for heat sterilization are: temperature 75-85℃, holding time 5-15 minutes, to inactivate the enzyme and prevent excessive degradation of the target component.

[0032] Preferably, in step (3) of preparing the gardenia extract, the extraction time is 45-65 min. Extraction at 75-85℃ can fully dissolve the flavonoids (such as rutin and quercetin) in the gardenia, because flavonoids contain polar groups such as phenolic hydroxyl groups and are easily soluble in water or ethanol-water mixed solvents.

[0033] Preferably, in step (4) of preparing the gardenia extract, the centrifugation speed is 4000-6000 r / min and the time is 5-15 min; the extract is filtered using a 0.45 μm microporous membrane to further remove small particles and obtain a clear extract.

[0034] Preferably, in step (5) of the preparation of the gardenia extract, the extract is concentrated under reduced pressure to 1 / 5 of its original volume, and the spray drying parameters are: inlet air temperature 175-185℃ and outlet air temperature 75-85℃.

[0035] Secondly, the present invention provides the application of the photoprotective whitening and repairing composition suitable for sensitive skin in the first aspect in the preparation of cosmetics.

[0036] Preferably, the cosmetic is a toner, lotion, cream, mask, serum, or gel, and the amount of the photoprotective whitening and repairing composition added is 1%-15% of the total weight of the cosmetic.

[0037] Thirdly, the present invention provides a whitening and repairing lotion comprising the following ingredients by weight percentage: 1%-15% of the photoprotective whitening and repairing composition suitable for sensitive skin in the first aspect, 0.1%-0.5% thickener, 1%-10% moisturizer, 0.01%-0.3% pH adjuster, 1%-5% emulsifier, 5%-15% oil and 0.5%-3% preservative, with the balance being deionized water.

[0038] Preferably, the thickener includes at least one of xanthan gum, acrylic (ester) copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl cellulose, carboxymethyl cellulose, and sodium alginate.

[0039] Preferably, the moisturizer includes at least one of allantoin, sodium polyacrylate, panthenol, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, and tremella polysaccharide.

[0040] Preferably, the pH adjuster includes at least one of arginine, citric acid, NaOH, and disodium EDTA.

[0041] Preferably, the emulsifier comprises at least one of polyglycerol-10 diisostearate, sucrose stearate, PEG-100 glyceryl stearate, glyceryl stearate citrate, cetearyl glucoside, cetyl alcohol, stearyl alcohol, cetearyl alcohol, and sodium stearoyl glutamate.

[0042] Preferably, the oil comprises at least one of caprylic / capric triglyceride, polydimethylsiloxane, jojoba oil, camellia seed oil, squalane, and baobab seed oil.

[0043] Preferably, the preservative includes at least one selected from 1,2-hexanediol, 1,2-pentanediol, ethylhexylglycerin, p-hydroxyacetophenone, phenoxyethanol, octanoyl hydroxamic acid, and sodium benzoate.

[0044] Fourthly, the present invention provides a method for preparing the whitening and repairing lotion of the third aspect, comprising the following steps:

[0045] (1) Mix the thickener and humectant evenly, add deionized water, homogenize at 70-80℃, keep warm for later use, and obtain pre-prepared component A.

[0046] (2) Mix the oil and emulsifier, homogenize at 70-80℃, keep warm for later use, and obtain the pre-prepared component B;

[0047] (3) Mix the pre-made component A and pre-made component B, homogenize them, and obtain the emulsion base material;

[0048] (4) Cool the emulsified base material to 45-55℃, add the photoprotective whitening and repairing composition, stir until the material is uniform, then add the preservative and pH adjuster, stop stirring, discharge the material, and obtain the whitening and repairing emulsion.

[0049] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0050] This invention incorporates oxidized resveratrol into the composition in the form of nanocrystals. Through nanocrystal technology, the particle size of oxidized resveratrol is reduced to 150-250nm after nano-sizing, significantly enhancing transdermal absorption and improving its water solubility and bioavailability. The composition of this invention, through the compounding of multi-target components, breaks through the efficacy limitations of traditional photoprotective whitening and repair products. This invention provides an efficient and safe solution for photoprotection, repair, and whitening of sensitive skin, and has significant application value and market prospects. Attached Figure Description

[0051] Figure 1 The particle size distribution of the oxidized resveratrol nanocrystals obtained in Example 1 is shown in Figure 1.

[0052] Figure 2 This is a comparison chart of the emulsion in Application Example 1 before and after use. Detailed Implementation

[0053] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0054] The raw materials used in the following examples and comparative examples are from the following sources:

[0055] Oxidized resveratrol: CAS No. 29700-22-9, purity ≥98%.

[0056] Hydroxypropyl methylcellulose: Manufacturer: Shandong Dechun Chemical Co., Ltd., Model No. 003;

[0057] Phosphatidylcholine: Manufacturer: Shanghai Aladdin Biochemical Technology Co., Ltd.; Model: L434221;

[0058] Lactobacillus freeze-dried powder: Manufacturer: Tianyi Biotechnology Co., Ltd.

[0059] Cellulase: Manufacturer: Shanghai Maclean Biochemical Technology Co., Ltd., Model: C758963-1g;

[0060] Pectinase: Manufacturer: Shanghai Maclean Biochemical Technology Co., Ltd., Model: P758970-500g;

[0061] Oat β-glucan: Manufacturer: Shanghai Yuanye Biotechnology Co., Ltd., Model: S11183-5g;

[0062] Unless otherwise specified, all other materials, reagents, etc. used in the examples and comparative examples are commercially available.

[0063] Examples 1-7 and Comparative Examples 1-4

[0064] The composition (by mass ratio) of the photoprotective whitening and repairing compositions suitable for sensitive skin in Examples 1-7 and Comparative Examples 1-4 is shown in Table 1. The total mass parts of each composition are 100 parts.

[0065] Table 1. Formulations of each group of photoprotective whitening and repairing compositions.

[0066]

[0067]

[0068] Specifically, the preparation method of the oxidized resveratrol nanocrystals in the compositions of Examples 1-7 and Comparative Examples 1-4 includes the following steps:

[0069] (1) Oxidized resveratrol and phosphatidylcholine are mixed in anhydrous ethanol and stirred until they are evenly dissolved to obtain the solvent phase; wherein, the ratio of oxidized resveratrol, phosphatidylcholine and anhydrous ethanol is 1g:0.2g:10mL, and the stirring speed is 450r / min.

[0070] (2) Hydroxypropyl methylcellulose was dissolved in water, heated to 40°C and then degassed by ultrasonication to obtain an antisolvent phase; a hydroxypropyl methylcellulose solution was obtained; wherein the ratio of hydroxypropyl methylcellulose to water was 1g:90mL.

[0071] (3) The solvent phase is added dropwise to the antisolvent phase while stirring. After the addition is complete, stirring is continued until precipitation is complete to obtain a suspension. The addition rate is 0.5 mL / min and the stirring speed is 1200 rpm.

[0072] (4) After homogenizing the suspension, centrifuge, discard the supernatant, resuspend in distilled water, and repeat the centrifugation-resuspending process to obtain a purified nanocrystalline suspension. Then, freeze-dry the resulting solution to obtain the nanocrystalline suspension. Figure 1 The oxidized resveratrol nanocrystals were homogenized under the following conditions: 125 MPa, 25 °C, three cycles; centrifugation was performed at 7500 rpm for 12 min.

[0073] The method for preparing the Lactobacillus fermentation product includes the following steps:

[0074] S1. Activation and Expansion Culture of Lactobacillus: Inoculate 5 ml LMR S medium with lyophilized Lactobacillus powder and incubate at 37°C for 16 hours until the logarithmic growth phase. Then, transfer the inoculum to 100 ml LMR S medium at a 5% inoculation rate and incubate at 37°C until the OD phase. 600 ≈1.0, to obtain seed culture; wherein, the viable bacteria count in the seed culture is 1×10 9 CFU / mL, MRS medium containing glucose 20g / L, peptone 10g / L, yeast extract 5g / L, beef extract 10g / L, dipotassium hydrogen phosphate 2g / L, triammonium citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, Tween-80 1mL / L, pH 6.3;

[0075] S2. Mix sodium alginate with distilled water until homogeneous, sterilize, and obtain sodium alginate solution; wherein, the ratio of sodium alginate to distilled water is 1g:25mL, the mixing temperature is 58℃, the sterilization temperature is 121℃, and the time is 15min.

[0076] S3. After the sodium alginate solution is cooled to below 40°C, it is stirred evenly with the seed liquid to obtain a mixed solution; wherein, the volume ratio of sodium alginate solution to seed liquid is 3:1.

[0077] S4. Using a sterile syringe (needle inner diameter 0.4 mm), drop the mixture into a pre-cooled calcium chloride solution at a distance of 30 cm to form spherical gel beads with a diameter of 2-3 mm. Allow the gel beads to stand in the calcium chloride solution for 40 min to harden. After filtration, wash three times with sterile physiological saline, centrifuge, discard the supernatant, and dry to obtain immobilized gel beads. The temperature of the calcium chloride solution is 4℃, the volume ratio of the mixture to the calcium chloride solution is 1:7, and the mass concentration of the calcium chloride solution is 2%. The centrifugation speed is 4000 r / min for 4 min, and the drying temperature is 72℃ for 4 min.

[0078] S5. The immobilized gel beads were added to the fermentation medium for anaerobic fermentation. Fermentation was terminated when pH ≤ 4.0 and lactic acid production no longer increased, yielding a fermentation mixture. The ratio of immobilized gel beads to fermentation medium was 1 g: 10 mL. The fermentation medium contained 50 g / L glucose, 10 g / L yeast extract, 10 g / L peptone, 70 g / L CaCO3, and 1 mL / L Tween-80, with a pH of 6.5. The anaerobic fermentation was carried out at 35℃ for 36 h.

[0079] S6. The fermentation mixture is subjected to solid-liquid separation. The gel beads are washed three times with sterile physiological saline to obtain supernatant and gel beads. The supernatant is concentrated by ultrafiltration, decolorized, and filtered to obtain the Lactobacillus fermentation product. The solid-liquid separation step involves centrifuging at 8000 r / min for 15 min. The washed gel beads can be reused for the next batch of fermentation (replaced with new gel beads after 3-4 reuses). The ultrafiltration concentration step is as follows: the supernatant is passed through an ultrafiltration membrane with a molecular weight cutoff of 10 kDa to remove large molecular impurities (such as undegraded proteins and polysaccharides) and concentrate the target product (lactic acid with a molecular weight of 90 Da). The decolorization step is as follows: 1 wt% food-grade activated carbon is added, and the mixture is stirred at 60℃ for 30 min. The pigments and odor substances are removed by filtration. A 0.22 μm polyethersulfone (PES) filter membrane is used for filtration.

[0080] The preparation method of the gardenia extract includes the following steps:

[0081] (1) Raw material pretreatment: Gardenia flowers are dried to a moisture content of ≤10%, pulverized and sieved to obtain gardenia flower powder;

[0082] (2) Compound enzymatic hydrolysis: Fresh gardenia flowers were selected, and impurities and non-medicinal parts (such as flower stalks) were removed. Gardenia flower powder was mixed with compound enzyme solution and subjected to ultrasonic enzymatic hydrolysis. After enzymatic hydrolysis, the enzyme was inactivated by heating to obtain a mixed solution. The solvent of the compound enzyme solution was distilled water. The compound enzyme solution contained a compound enzyme consisting of cellulase and pectinase in a mass ratio of 1:1. The mass concentration of the compound enzyme solution was 0.8%. The material-liquid ratio of gardenia flower powder to compound enzyme solution was 1g:22mL. 0.1mol / L citrate-sodium citrate buffer solution was added to adjust the pH of the enzymatic hydrolysate to 4.5. The enzyme inactivation temperature was 80℃ and the time was 10min.

[0083] (3) Extraction: The mixture is extracted at 80°C for 60 min to obtain the extract;

[0084] (4) Solid-liquid separation and purification: Centrifuge the extract, take the supernatant, filter, and obtain the extract; wherein, the centrifugation speed is 5000 r / min and the time is 10 min, and the filter is filtered using a 0.45 μm microporous membrane;

[0085] (5) Concentration and drying: The extract is concentrated to 1 / 5 of its original volume and spray-dried to obtain the gardenia extract in powder form; wherein the inlet air temperature of the spray drying is 180°C and the outlet air temperature is 80°C.

[0086] Example 8

[0087] The only difference between Example 8 and Example 1 is that the preparation method of the oxidized resveratrol nanocrystals includes the following steps:

[0088] (1) Oxidized resveratrol and phosphatidylcholine are mixed in anhydrous ethanol and stirred until they are evenly dissolved to obtain the solvent phase; wherein, the ratio of oxidized resveratrol, phosphatidylcholine and anhydrous ethanol is 1g:0.1g:8mL, and the stirring speed is 400r / min.

[0089] (2) Hydroxypropyl methylcellulose was dissolved in water, heated to 35°C and then degassed by ultrasonication to obtain an antisolvent phase; a hydroxypropyl methylcellulose solution was obtained; wherein the ratio of hydroxypropyl methylcellulose to water was 1 g: 80 mL.

[0090] (3) The solvent phase is added dropwise to the antisolvent phase while stirring. After the addition is complete, stirring is continued until precipitation is complete to obtain a suspension. The addition rate is 0.5 mL / min and the stirring speed is 1000 rpm.

[0091] (4) After homogenizing the suspension, centrifuge, discard the supernatant, resuspend the precipitate in distilled water and repeat the centrifugation-resuspending process to obtain the purified nanocrystalline suspension. Then freeze-dry the suspension to obtain the oxidized resveratrol nanocrystals. The homogenization conditions are: 125 MPa, 25 °C, three cycles; the centrifugation speed is 7000 rpm and the time is 15 min.

[0092] The method for preparing the Lactobacillus fermentation product includes the following steps:

[0093] S1. Activation and Expansion Culture of Lactobacillus: Inoculate 5 ml LMR S medium with lyophilized Lactobacillus powder and incubate at 37°C for 12 hours until the logarithmic growth phase. Then, transfer 5% of the inoculum to 100 ml LMR S medium and incubate at 37°C until the OD phase. 600 ≈1.0, to obtain seed culture; wherein, the viable bacteria count in the seed culture is 1×10 8 CFU / mL, MRS medium containing glucose 20g / L, peptone 10g / L, yeast extract 5g / L, beef extract 10g / L, dipotassium hydrogen phosphate 2g / L, triammonium citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, Tween-80 1mL / L, pH 6.2;

[0094] S2. Mix sodium alginate with distilled water until homogeneous, sterilize, and obtain sodium alginate solution; wherein, the ratio of sodium alginate to distilled water is 1g:20mL, the mixing temperature is 55℃, the sterilization temperature is 115℃, and the time is 15min.

[0095] S3. After the sodium alginate solution is cooled to below 40°C, it is stirred evenly with the seed liquid to obtain a mixed solution; wherein the volume ratio of sodium alginate solution to seed liquid is 2:1.

[0096] S4. Using a sterile syringe (needle inner diameter 0.4 mm), drop the mixture into the pre-cooled calcium chloride solution at a distance of 30 cm to form spherical gel beads with a diameter of 2-3 mm. Allow the gel beads to stand in the calcium chloride solution for 30 min to harden. After filtration, wash three times with sterile physiological saline, centrifuge, discard the supernatant, and dry to obtain immobilized gel beads. The temperature of the calcium chloride solution was 4℃, the volume ratio of the mixture to the calcium chloride solution was 1:6, and the mass concentration of the calcium chloride solution was 1%. The centrifugation speed was 3000 r / min for 5 min, and the drying temperature was 75℃ for 3 min.

[0097] S5. The immobilized gel beads were added to the fermentation medium for anaerobic fermentation. Fermentation was terminated when pH ≤ 4.0 and lactic acid production no longer increased, yielding a fermentation mixture. The ratio of immobilized gel beads to fermentation medium was 1 g: 9 mL. The fermentation medium contained 50 g / L glucose, 10 g / L yeast extract, 10 g / L peptone, 70 g / L CaCO3, and 1 mL / L Tween-80, with a pH of 6.5. The anaerobic fermentation was carried out at 30℃ for 36 h.

[0098] S6. Solid-liquid separation of the fermentation mixture: Wash the gel beads three times with sterile physiological saline to obtain supernatant and gel beads; Perform ultrafiltration concentration, decolorization, and filtration on the supernatant to obtain the Lactobacillus fermentation product; The solid-liquid separation step involves centrifugation at 7000 r / min for 15 min. The washed gel beads can be reused for the next batch of fermentation (replace with new gel beads after 3-4 reuses); The ultrafiltration concentration step is as follows: Pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 10 kDa to remove macromolecular impurities (such as undegraded proteins and polysaccharides), while concentrating the target product (lactic acid molecular weight 90 Da); The specific decolorization step is as follows: Add 1 wt% food-grade activated carbon, stir at 55℃ for 30 min, and filter to remove pigments and odor substances; Filter using a 0.22 μm polyethersulfone (PES) membrane.

[0099] The preparation method of the gardenia extract includes the following steps:

[0100] (1) Raw material pretreatment: Gardenia flowers are dried to a moisture content of ≤10%, pulverized and sieved to obtain gardenia flower powder;

[0101] (2) Compound enzymatic hydrolysis: Fresh gardenia flowers are selected, and impurities and non-medicinal parts (such as flower stalks) are removed. Gardenia flower powder is mixed with compound enzyme solution and subjected to ultrasonic enzymatic hydrolysis. After enzymatic hydrolysis, the enzyme is inactivated by heating to obtain a mixed solution. The solvent of the compound enzyme solution is distilled water. The compound enzyme solution includes a compound enzyme of cellulase and pectinase in a mass ratio of 1:0.8. The mass concentration of the compound enzyme solution is 0.5-1%. The material-to-liquid ratio of gardenia flower powder to compound enzyme solution is 1g:15mL. 0.1mol / L citrate-sodium citrate buffer solution is added to adjust the pH of the enzymatic hydrolysate to 4.5. The enzyme inactivation temperature is 75℃ and the time is 15min.

[0102] (3) Extraction: The mixture is extracted at 75°C for 65 min to obtain the extract;

[0103] (4) Solid-liquid separation and purification: Centrifuge the extract, take the supernatant, filter, and obtain the extract; the centrifugation speed is 4000 r / min and the time is 15 min, and the filter is filtered with a 0.45 μm microporous membrane.

[0104] (5) Concentration and drying: The extract is concentrated to 1 / 5 of its original volume and spray-dried to obtain the gardenia extract in powder form; wherein the inlet air temperature of the spray drying is 175°C and the outlet air temperature is 75°C.

[0105] Example 9

[0106] The preparation method of the oxidized resveratrol nanocrystals includes the following steps:

[0107] (1) Oxidized resveratrol and phosphatidylcholine are mixed in anhydrous ethanol and stirred until they are evenly dissolved to obtain the solvent phase; wherein, the ratio of oxidized resveratrol, phosphatidylcholine and anhydrous ethanol is 1g:0.3g:12mL, and the stirring speed is 500r / min.

[0108] (2) Hydroxypropyl methylcellulose was dissolved in water, heated to 45°C and then degassed by ultrasonication to obtain an antisolvent phase; a hydroxypropyl methylcellulose solution was obtained; wherein the ratio of hydroxypropyl methylcellulose to water was 1g:100mL;

[0109] (3) The solvent phase is added dropwise to the antisolvent phase while stirring. After the addition is complete, stirring is continued until precipitation is complete to obtain a suspension. The addition rate is 0.5 mL / min and the stirring speed is 1500 rpm.

[0110] (4) After homogenizing the suspension, centrifuge, discard the supernatant and resuspend in distilled water, and repeat centrifugation-resuspending to obtain the purified nanocrystal suspension. Then freeze-dry to obtain the oxidized resveratrol nanocrystals. The homogenization conditions are: 125 MPa, 25 °C, three cycles; the centrifugation speed is 8000 rpm and the time is 10 min.

[0111] The method for preparing the Lactobacillus fermentation product includes the following steps:

[0112] S1. Activation and Expansion Culture of Lactobacillus: Inoculate 5 ml LMR S medium with lyophilized Lactobacillus powder and incubate at 37°C for 18 hours until the logarithmic growth phase. Then, transfer 5% of the inoculum to 100 ml LMR S medium and incubate at 37°C until the OD phase. 600 ≈1.0, to obtain seed culture; wherein, the viable bacteria count in the seed culture is 2×10 9 CFU / mL, MRS medium containing glucose 20g / L, peptone 10g / L, yeast extract 5g / L, beef extract 10g / L, dipotassium hydrogen phosphate 2g / L, triammonium citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, Tween-80 1mL / L, pH 6.4;

[0113] S2. Mix sodium alginate with distilled water until homogeneous, sterilize, and obtain sodium alginate solution; wherein, the ratio of sodium alginate to distilled water is 1g:30mL, the mixing temperature is 60℃, the sterilization temperature is 121℃, and the time is 10min.

[0114] S3. After the sodium alginate solution is cooled to below 40°C, it is stirred evenly with the seed liquid to obtain a mixed solution; wherein, the volume ratio of sodium alginate solution to seed liquid is 5:1.

[0115] S4. Using a sterile syringe (needle inner diameter 0.4 mm), drop the mixture into the pre-cooled calcium chloride solution at a distance of 30 cm to form spherical gel beads with a diameter of 2-3 mm. Allow the gel beads to stand in the calcium chloride solution for 60 min to harden. After filtration, wash three times with sterile physiological saline, centrifuge, discard the supernatant, and dry to obtain immobilized gel beads. The temperature of the calcium chloride solution was 4℃, the volume ratio of the mixture to the calcium chloride solution was 1:8, and the mass concentration of the calcium chloride solution was 3%. The centrifugation speed was 3000 r / min for 5 min, and the drying temperature was 70℃ for 5 min.

[0116] S5. The immobilized gel beads were added to the fermentation medium for anaerobic fermentation. Fermentation was terminated when pH ≤ 4.0 and lactic acid production no longer increased, yielding a fermentation mixture. The ratio of immobilized gel beads to fermentation medium was 1 g: 11 mL. The fermentation medium contained 50 g / L glucose, 10 g / L yeast extract, 10 g / L peptone, 70 g / L CaCO3, and 1 mL / L Tween-80, with a pH of 6.5. The anaerobic fermentation was carried out at 37°C for 24 hours.

[0117] S6. Solid-liquid separation of the fermentation mixture: Wash the gel beads three times with sterile physiological saline to obtain supernatant and gel beads; Perform ultrafiltration concentration, decolorization, and filtration on the supernatant to obtain the Lactobacillus fermentation product; The solid-liquid separation step involves centrifuging at 8000 r / min for 10 min. The washed gel beads can be reused for the next batch of fermentation (replace with new gel beads after 3-4 reuses); The ultrafiltration concentration step is as follows: Pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 10 kDa to remove large molecular impurities (such as undegraded proteins and polysaccharides), while concentrating the target product (lactic acid molecular weight 90 Da); The specific decolorization step is as follows: Add 1 wt% food-grade activated carbon, stir at 60℃ for 30 min, and filter to remove pigments and odor substances; Filter using a 0.22 μm polyethersulfone (PES) membrane;

[0118] The preparation method of the gardenia extract includes the following steps:

[0119] (1) Raw material pretreatment: Gardenia flowers are dried to a moisture content of ≤10%, pulverized and sieved to obtain gardenia flower powder;

[0120] (2) Compound enzymatic hydrolysis: Fresh gardenia flowers are selected, and impurities and non-medicinal parts (such as flower stalks) are removed. Gardenia flower powder is mixed with compound enzyme solution and subjected to ultrasonic enzymatic hydrolysis. After enzymatic hydrolysis, the enzyme is inactivated by heating to obtain a mixed solution. The solvent of the compound enzyme solution is distilled water. The compound enzyme solution includes a compound enzyme of cellulase and pectinase in a mass ratio of 1:1.1. The mass concentration of the compound enzyme solution is 0.5-1%. The material-liquid ratio of gardenia flower powder to compound enzyme solution is 1g:25mL. 0.1mol / L citrate-sodium citrate buffer solution is added to adjust the pH of the enzymatic hydrolysate to 5.0. The enzyme inactivation temperature is 85℃ and the time is 5min.

[0121] (3) Extraction: The mixture is extracted at 85°C for 45 min to obtain the extract;

[0122] (4) Solid-liquid separation and purification: Centrifuge the extract, take the supernatant, filter, and obtain the extract; the centrifugation speed is 6000 r / min and the time is 5 min, and the filter is filtered with a 0.45 μm microporous membrane.

[0123] (5) Concentration and drying: The extract is concentrated to 1 / 5 of its original volume and spray-dried to obtain the gardenia extract in powder form; wherein the inlet air temperature of the spray drying is 185°C and the outlet air temperature is 85°C.

[0124] Test Example 1: Solubility and stability tests of different oxidized resveratrol forms

[0125] The oxidized resveratrol nanocrystals prepared in Example 1 and untreated ordinary oxidized resveratrol powder (original particle size approximately 20-50 μm) were tested:

[0126] 1. The specific steps of the solubility test are as follows: weigh equal amounts of two groups of samples (100mg) and add 10mL of aqueous phase (pH5.5 phosphate buffer) and 10mL of oil phase (medium chain triglycerides). After sonication for 30 minutes, centrifuge at 10000rpm for 15 minutes. Take the supernatant and quantitatively analyze the content of oxidized resveratrol by HPLC.

[0127] 2. The specific steps of the stability test are as follows: the two groups of samples are placed at 5℃ (protected from light) and 45℃ (accelerated test) and samples are taken regularly to detect the change in particle size and the degradation rate of active ingredients. The results show that nanocrystal technology can significantly enhance the solubility of oxidized resveratrol and its resistance to heat and oxidation degradation by reducing the particle size to the nanoscale and combining it with the physical encapsulation effect of phospholipid-hydroxypropyl methylcellulose composite carrier.

[0128] Table 2. Results of solubility and stability experiments

[0129]

[0130]

[0131] As shown in Table 2, the solubility of oxidized resveratrol nanocrystals in both aqueous and oil phases is 10-12 times higher than that of oxidized resveratrol powder. The thermal and cold stability of oxidized resveratrol nanocrystals are also higher than that of oxidized resveratrol powder. This is likely because nanocrystal technology, by reducing the particle size to the nanoscale and combining it with the physical encapsulation effect of the phospholipid-hydroxypropyl methylcellulose composite carrier, can significantly enhance the solubility and resistance to thermal and oxidative degradation of oxidized resveratrol.

[0132] Test Example 2: Permeation Effect Test of Different Oxidized Resveratrol Forms

[0133] Transdermal absorption and skin retention experiments were conducted using the Franz diffusion cell method: Ex vivo skin samples (200–400 μm thick) from Bama miniature pigs were immobilized in the diffusion cell. 0.5 g of oxidized resveratrol nanocrystals or oxidized resveratrol powder was added to the supply cell. The receiving cell contained 5 mL of pH 7.4 PBS with 0.5% sodium azide. The experiment was conducted in the dark under stirring conditions at 32 ± 0.5 °C and 600 rpm. Samples of 0.5 mL were taken at 1, 2, 4, 6, 8, 12, and 24 hours (with simultaneous fluid replenishment). The drug concentration in the receiving solution was determined by HPLC, and the cumulative permeation (Q) was calculated. n After the experiment, the drug retained in the skin was extracted with ethanol, and the amount retained in the skin was measured.

[0134] Table 3 Results of transdermal absorption and skin retention experiments

[0135]

[0136] As shown in Table 3, the cumulative transdermal absorption and retention of oxidized resveratrol nanocrystals after 24 hours were significantly higher than those of untreated ordinary oxidized resveratrol powder, indicating that oxidized resveratrol nanocrystalization significantly enhanced the transdermal absorption and skin-targeted deposition of the drug.

[0137] Test Example 3: Photooxidation Resistance Test of the Composition

[0138] This test case uses a 3D full-thickness skin model detection kit (EpiDerm FT). TM To simulate human skin structure, a control group (UV irradiation), a negative control group (no treatment), and a sample group (composition samples of Examples 1-9 and Comparative Examples 1-4 + UV irradiation) were set up. The sample group required the following pretreatment: 2 mg of sample was evenly applied to the model surface and incubated for 24 hours. The UV irradiation conditions for both the control and sample groups were UVB lamps (30 mJ / cm²). 2 Irradiation for 15 minutes induced oxidative stress, followed by the addition of DCFH-DA fluorescent probe to label reactive oxygen species (ROS). The fluorescence intensity of the entire model layer was detected by a fluorescent microplate reader to quantify the ROS level. The culture supernatant was collected and the content of oxidative markers (MDA) was measured by ELISA.

[0139] The calculation formula is as follows:

[0140] ROS inhibition rate (%) = [1 - (ROS fluorescence intensity of sample group - ROS fluorescence intensity of negative control group) / (ROS fluorescence intensity of control group - ROS fluorescence intensity of negative control group)] × 100%;

[0141] MDA inhibition rate (%) = [1 - (MDA concentration in sample group / MDA concentration in control group)] × 100%. The results are shown in Table 4.

[0142] Table 4. ROS inhibition rate and MDA inhibition rate of each composition

[0143] Group / Performance ROS inhibition rate (%) MDA inhibition rate (%) Example 1 68.6 55.2 Example 2 66.0 53.6 Example 3 68.2 54.3 Example 4 64.6 52.9 Example 5 65.8 53.2 Example 6 59.4 49.3 Example 7 62.5 51.2 Example 8 67.4 53.8 Example 9 68.9 54.0 Comparative Example 1 37.1 28.7 Comparative Example 2 43.6 33.5 Comparative Example 3 45.0 34.3 Comparative Example 4 40.6 31.1

[0144] The higher the ROS inhibition rate and MDA inhibition rate, the better the composition exerts its anti-photooxidation effect through multiple pathways, such as scavenging ROS and enhancing antioxidant enzyme activity.

[0145] As shown in Table 4, and in conjunction with the data from Examples 1-7, when the weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation product is between (0.5-1.2):

[0146] When the range of (0.1-0.2):(0.4-0.5):(2-3) is within the range of (0.1-0.2):(0.4-0.5):(2-3), the composition exhibits superior photo-oxidation protection properties.

[0147] Based on the data from Example 1 and Comparative Examples 1-4, it can be seen that the anti-photooxidation performance of the compositions of Comparative Examples 1-4, which lack one of the following: oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation product, is significantly lower than that of Example 1. This may be because oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation product have a synergistic effect on anti-photooxidation.

[0148] Test Example 4: In vitro whitening test of the composition

[0149] This test example uses a tyrosinase inhibition experiment to verify the whitening effect of the composition. The specific steps are as follows: a PBS buffer at pH 6.8 is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate; L-tyrosine is diluted to 3 mg / mL with PBS as a substrate; mushroom tyrosinase is dissolved in PBS to prepare a 100 U / mL enzyme solution; the test samples (compositions of Examples 1-9 and Comparative Examples 1-4) are diluted to the target concentration with PBS. The buffer, test sample, and L-tyrosine are incubated at 37°C for 10 min, followed by the addition of tyrosinase to each group. The reaction is continued at 37°C for another 5 min, and the absorbance is measured at 475 nm. Specific groupings are shown in Table 5. The inhibition rate is calculated using a blank control group after zeroing the instrument. The calculation formula is:

[0150] Inhibition rate (%) = [1 - (OD of sample reaction group - OD of sample background group) / (OD of solvent reaction group - OD of solvent background group)] × 100%. The experimental results are shown in Table 6.

[0151] Table 5. Grouping of Tyrosinase Inhibition Experiments

[0152]

[0153] Table 6. Tyrosinase inhibition rate of each group of compositions

[0154]

[0155]

[0156] As shown in Table 6, the higher the tyrosinase inhibition rate, the better the whitening performance of the composition, which is similar to the trend of anti-photooxidation performance.

[0157] Based on the data from Examples 1-7, it can be seen that when the weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation product is in the range of (0.5-1.2):(0.1-0.2):(0.4-0.5):(2-3), the whitening performance of the composition is at a relatively high level.

[0158] Based on the data from Example 1 and Comparative Examples 1-4, it can be seen that the anti-photooxidation performance of the compositions of Comparative Examples 1-4, which lack one of the following: oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation product, is significantly lower than that of Example 1. This may be because oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation product have a synergistic whitening effect.

[0159] Application Example 1-9 and Comparative Application Example 1-4

[0160] The compositions of Examples 1-9 and Comparative Examples 1-4 were added to the whitening repair lotion at a concentration of 5 wt% to obtain the whitening repair lotion of Application Examples 1-9 and Comparative Application Examples 1-4. The formulations are shown in Table 7.

[0161] The preparation methods of the whitening and repairing lotions used in Application Examples 1-7 and Comparative Application Examples 1-4 include the following steps:

[0162] (1) Mix the thickener and humectant evenly, add deionized water, heat at 75°C water bath temperature for 10 minutes, then transfer to a homogenizer and homogenize at 4000 rpm for 5 minutes. Keep warm for later use to obtain pre-prepared component A.

[0163] (2) Take the oil and emulsifier in a beaker, heat to 75°C, dissolve evenly, keep warm for later use, and obtain the pre-prepared component B.

[0164] (3) Heat the pre-prepared component A to 75°C, add the pre-prepared component B preheated to 75°C in a homogenizer at 4000 rpm, homogenize for 5 min, and obtain the emulsion base material;

[0165] (4) Stir the emulsified base material and cool it down to 50°C. Add the photoprotective whitening and repairing composition and continue stirring until the material is uniform. Then add the preservative and pH adjuster to adjust the pH to 6.0. Stop stirring and discharge the material to obtain the whitening and repairing emulsion.

[0166] Table 7 shows the formulations of whitening and repairing lotions in Application Examples 1-9 and Comparative Application Examples 1-4.

[0167]

[0168] Comparative Application Example 5

[0169] Compared to Application Example 5, the whitening and repairing lotion does not contain the photoprotective whitening and repairing composition, but uses an equal amount of deionized water instead of the composition, and the preparation method is the same as Application Example 1.

[0170] Test Example 5: Human Patch Test of Emulsion

[0171] Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal amounts (0.5g-0.6g) of test samples (whitening and repairing lotions prepared in Application Examples 1-9 and Comparative Application Examples 1-5) were placed in a specific patch applicator. The patch was then applied to the volunteers' arms with hypoallergenic adhesive tape, with seven samples applied to each arm. The patches were gently pressed to ensure even application to the skin and left on for 24 hours. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5h, 24h, and 48h. The severity of adverse skin reactions is shown in Table 8 below.

[0172] Table 8. Adverse Reaction Grades of the Skin

[0173]

[0174] After testing, the whitening and repairing lotions provided in Application Examples 1-9 and Comparative Application Examples 1-5 all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0175] Test Example 6: Whitening and Barrier Repair Effects of Whitening Repair Lotion on the Human Body

[0176] (1) Experimental basis

[0177] The human efficacy evaluation test method shall be followed in accordance with the "Technical Specifications for Cosmetic Safety" (2015 edition).

[0178] (2) Subject screening

[0179] Inclusion criteria: We are recruiting Asian adult female volunteers aged 45-60 who meet the following conditions:

[0180] a) Screening healthy volunteers with freckles or dull facial skin;

[0181] b) A self-reported history of skin allergies (confirmed by a dermatologist);

[0182] c) No history of serious systemic diseases or skin diseases.

[0183] Exclusion criteria: pregnant / lactating women, individuals with severe allergies, and individuals who have participated in other clinical trials within the past 3 months.

[0184] Number of participants: A total of 70 qualified volunteers were included and divided into 14 groups of 5 people each using a random number table.

[0185] (3) Sample application method

[0186] Test samples: Whitening and repairing lotions prepared in Application Examples 1-9 and Comparative Application Examples 1-5 (double-blind method numbering), wherein the lotion prepared in Comparative Application Example 5 is the blank control group, and the other application examples and comparative application examples are the sample groups.

[0187] How to use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed.

[0188] (4) Testing cycle and process

[0189] Test period: 14 or 28 days (D0, D) 14 D 28 ).

[0190] Visit time points: D0 (baseline period), D 14 D 28 (At the end of the test) Instrument testing will be conducted.

[0191] Preparation before testing:

[0192] a) After the participants arrived, they used a uniform, non-irritating facial cleanser to clean their faces;

[0193] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0194] c) Keep your eyes closed and relax during the test to avoid facial expressions and movements that may interfere with the process.

[0195] (5) Test Indicators

[0196] Whitening effect:

[0197] a) Skin color: The absorbance and reflectance of the skin surface are measured using a tristimulus colorimeter or a narrow-band reflectance spectrophotometer. The ITA° value (skin brightness, the higher the value, the brighter the skin) and ΔE value (overall color change, the higher the value, the more significant the whitening effect) in the Lab colorimetry system are calculated.

[0198] b) Melanin Index: The melanin content of the skin is measured using a narrow-band reflectance spectrophotometer, and the melanin index is calculated. The lower the melanin index, the higher the skin whiteness.

[0199] Skin barrier repair effect:

[0200] The TEWL value of the skin surface is measured using a skin moisture loss tester to assess the moisturizing function of the skin barrier. The lower the TEWL value, the better the skin barrier.

[0201] The facial skin condition was tested on the 14th and 28th days after product use to comprehensively evaluate the whitening efficacy and TEWL value of the product.

[0202] The formulas for calculating the evaluation parameters are as follows:

[0203] (T14, 28) ΔE value improvement rate = (ΔE value) Tn -ΔE value T0 ) / ΔE value T0 ×100%; where, T n =T 14 T 28 .

[0204] (T14, 28) ITA° value improvement rate = (ITA°) Tn -ITA° T0 ) / ITA° T0 ×100%; where, T n =T 14 T 28 .

[0205] (T14, 28) Melanin Index Improvement Rate = (Melanin Index) T0 -Melanin Index Tn Melanin Index T0 ×100%; where, T n =T 14 T 28 .

[0206] (T14, 28) TEWL value improvement rate = (TEWL value) T0 -TEWL value Tn ) / TEWL value T0 ×100%; where, T n =T 14 T 28 The data is shown in Table 9.

[0207] Table 9. Human test results of each group of whitening and repairing lotions.

[0208]

[0209]

[0210] As shown in Table 8, combining the performance data of the emulsions from Application Example 1 and Comparative Application Examples 1-4, it can be seen that oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan, and lactobacillus fermentation products can synergistically improve facial skin barrier damage / fragility, sagging, and fine lines, and enhance the skin's photoprotective ability.

[0211] Combination Figure 2 It can be seen that after using the lotion of Application Example 1 for 14 days, the subjects' facial luster, brightness and whiteness were improved, indicating that the lotion of Application Example 1 has a good whitening and repairing effect.

[0212] In summary, this invention, through the combination of multi-target components, breaks through the efficacy limitations of traditional photoprotective whitening and repair products, and provides a highly efficient and safe solution for photoprotection, repair, and whitening of sensitive skin.

[0213] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A photoprotective, whitening, and repairing composition suitable for sensitive skin, characterized in that, It comprises the following components: oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product, wherein the weight ratio of oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product is (0.01-6):(0.01-0.5):(0.01-2):(0.1-10); The preparation method of the oxidized resveratrol nanocrystals includes the following steps: (1) Mix resveratrol oxide and phosphatidylcholine in anhydrous ethanol and stir until they are evenly dissolved to obtain the solvent phase; wherein, the ratio of resveratrol oxide, phosphatidylcholine and anhydrous ethanol is 1g:(0.1-0.3)g:(8-12)mL. (2) Dissolve hydroxypropyl methylcellulose in water, heat to 35-45℃ and then degas by ultrasonication to obtain the antisolvent phase; wherein, the ratio of hydroxypropyl methylcellulose to water is 1g:(80-100)mL; (3) Add the solvent phase dropwise to the antisolvent phase while stirring. After the addition is complete, continue stirring until precipitation is complete to obtain a suspension. (4) After homogenizing the suspension, centrifuge, discard the supernatant and resuspend in distilled water, and repeat centrifugation-resuspending to obtain the purified nanocrystal suspension. Then freeze-dry to obtain the oxidized resveratrol nanocrystals. The method for preparing the Lactobacillus fermentation product includes the following steps: S1. Activation and Expansion Culture of the Strain: Lyophilized Lactobacillus powder was inoculated into MRS medium for culture to obtain a seed culture; the viable count in the seed culture was 1 × 10⁻⁶. 8 -2×10 9 CFU / mL; S2. Mix sodium alginate with distilled water evenly and sterilize to obtain sodium alginate solution; wherein, the ratio of sodium alginate to distilled water is 1g:(20-30)mL; S3. Stir the sodium alginate solution and the seed liquid evenly to obtain a mixed solution; wherein the volume ratio of sodium alginate solution to seed liquid is (2-5):1; S4. Using a sterile syringe, drop the mixture into a pre-cooled calcium chloride solution to form spherical gel beads with a diameter of 2-3 mm. The gel beads are allowed to stand and harden in the calcium chloride solution. After filtration, washing, centrifugation, discarding the supernatant, and drying, immobilized gel beads are obtained. S5. Add the immobilized gel beads to the fermentation medium for anaerobic fermentation. Terminate the fermentation when pH ≤ 4.0 and lactic acid production no longer increases, and obtain a fermentation mixture. The ratio of immobilized gel beads to fermentation medium is 1 g: (9-11) mL. S6. The fermentation mixture is subjected to solid-liquid separation to obtain supernatant and gel beads. The supernatant is then subjected to ultrafiltration concentration, decolorization and filtration to obtain the lactobacillus fermentation product.

2. The photoprotective, whitening, and repairing composition suitable for sensitive skin as described in claim 1, characterized in that, The weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product is (0.1-3):(0.05-0.3):(0.1-1):(1-5).

3. The photoprotective, whitening, and repairing composition suitable for sensitive skin as described in claim 1, characterized in that, The weight ratio of the oxidized resveratrol nanocrystals, gardenia extract, oat β-glucan and lactobacillus fermentation product is (0.5-1.2):(0.1-0.2):(0.4-0.5):(2-3).

4. The photoprotective, whitening, and repairing composition suitable for sensitive skin as described in claim 1, characterized in that, The preparation method of the gardenia extract includes the following steps: (1) Raw material pretreatment: Gardenia flowers are dried to a moisture content of ≤10%, pulverized and sieved to obtain gardenia flower powder; (2) Compound enzymatic hydrolysis: Gardenia powder is mixed with compound enzyme solution and subjected to ultrasonic enzymatic hydrolysis. After enzymatic hydrolysis, the enzyme is inactivated by heating to obtain a mixed solution. The solvent of the compound enzyme solution is an aqueous solution. The compound enzyme solution includes a compound enzyme of cellulase and pectinase in a mass ratio of 1:(0.8-1.1). The mass concentration of the compound enzyme in the compound enzyme solution is 0.5-1%. The material-liquid ratio of gardenia powder to compound enzyme solution is 1g:(15-25)mL. (3) Extraction: The mixture is heated at 75-85℃ to extract the extract. (4) Solid-liquid separation and purification: Centrifuge the extract, collect the supernatant, filter, and obtain the extract; (5) Concentration and drying: The extract is concentrated and dried to obtain powdered gardenia extract.

5. The use of the photoprotective whitening and repairing composition suitable for sensitive skin according to any one of claims 1-4 in the preparation of cosmetics.

6. The application of the photoprotective, whitening, and repairing composition for sensitive skin as described in claim 5 in the preparation of cosmetics, characterized in that, The cosmetic is a toner, lotion, cream, mask, or gel, and the amount of the photoprotective whitening and repairing composition added is 1%-15% of the total weight of the cosmetic.

7. A whitening and repairing lotion, characterized in that, The ingredients comprise the following ingredients by weight percentage: 1%-15% of the photoprotective whitening and repairing composition suitable for sensitive skin according to any one of claims 1-4, 0.1%-0.5% thickener, 1%-10% moisturizer, 0.01%-0.3% pH adjuster, 1%-5% emulsifier, 5%-15% oil and fat, and 0.5%-3% preservative, with the balance being deionized water.

8. The whitening and repairing lotion as described in claim 7, characterized in that, The raw material is selected from at least one of (a)-(f): (a) The thickener comprises at least one of xanthan gum, acrylic (ester) copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl cellulose, carboxymethyl cellulose, and sodium alginate; (b) The moisturizer includes at least one of allantoin, sodium polyacrylate, panthenol, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, and tremella polysaccharide; (c) The pH adjuster includes at least one of arginine, citric acid, NaOH, and disodium EDTA; (d) The emulsifier comprises at least one of polyglycerol-10 diisostearate, sucrose stearate, PEG-100 glyceryl stearate, glyceryl stearate citrate, cetearyl glucoside, cetyl alcohol, stearyl alcohol, cetearyl alcohol, and sodium stearoyl glutamate. (e) The oils include at least one of caprylic / capric triglyceride, polydimethylsiloxane, jojoba oil, camellia seed oil, squalane, and baobab seed oil; (f) The preservatives include at least one of 1,2-hexanediol, 1,2-pentanediol, ethylhexylglycerin, p-hydroxyacetophenone, phenoxyethanol, octanoyl hydroxamic acid, and sodium benzoate.

9. The method for preparing the whitening and repairing lotion according to claim 8, characterized in that, Includes the following steps: (1) Mix the thickener and humectant evenly, add deionized water, homogenize at 70-80℃, keep warm for later use, and obtain pre-prepared component A. (2) Mix the oil and emulsifier, homogenize at 70-80℃, keep warm for later use, and obtain the pre-prepared component B; (3) Mix the pre-made component A and pre-made component B, homogenize them, and obtain the emulsion base material; (4) Cool the emulsified base material to 45-55℃, add the photoprotective whitening and repairing composition, stir until the material is uniform, then add the preservative and pH adjuster, stop stirring, discharge the material, and obtain the whitening and repairing emulsion.

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