Hybridoma cell strain secreting anti-captopril monoclonal antibody and application thereof
By providing hybridoma cell lines that secrete monoclonal antibodies against captopril, the problems of expensive and insufficient sensitivity of existing captopril detection equipment have been solved, achieving efficient and sensitive captopril detection and ensuring medication safety.
CN121065103APending Publication Date: 2025-12-05WUXI DITENGMIN BIOTECHNOLOGY CO LTD +1
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Patent Information
- Application Number
- CN202511037287.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-28
- Publication Date
- 2025-12-05
AI Technical Summary
Technical Problem
Existing captopril detection methods require specialized equipment and are costly, making it difficult to meet the needs of clinical real-time monitoring and batch sample testing, and they also suffer from insufficient detection sensitivity.
Method used
A hybridoma cell line secreting anti-captopril monoclonal antibody and its application are provided. A captopril detection method is established by enzyme-linked immunosorbent assay (ELISA). The antibody secreted by this cell line has high sensitivity and specificity and can be used to detect captopril in biological samples.
Benefits of technology
This technology enables highly accurate detection of captopril, providing an efficient immune detection tool and ensuring the safety and effectiveness of clinical medication.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention relates to a hybridoma cell strain secreting an anti-captopril monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of drug safety immunoassay. The hybridoma cell strain secreting the anti-monoclonal antibody is preserved in China General Microbiological Culture Collection Center (CGMCC) on the year, the preservation number is CGMCC No.46502, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the hybridoma cell strain is classified and named as a monoclonal cell strain. The captopril monoclonal antibody cell strain obtained by the invention can be used for drug quality detection and captopril residue detection in a biological sample, has better detection sensitivity (IC50 value is 3.433 ng / mL) and specificity (the cross-over rate for related structural analogues is less than 1%) to captopril, and can realize detection of captopril in drugs and biological samples; and an efficient immunodetection tool is provided for clinical medication detection and drug quality control, the clinical medication safety and effectiveness are guaranteed in an assisted manner, and the kit has a definite practical application value.
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