A time-resolved fluorescence detection device and method

Abstract

The application provides a time-resolved fluorescence detection device and a detection method, relates to the technical field of fluorescence detection, and comprises a detection box, the inside of the detection box is provided with a pipettor, a test tube rack, a test card, a two-axis moving table and a linear moving table, the inside of the detection box is further provided with a detection cavity, the inside of the detection cavity is provided with an incubation box, the inside of the detection cavity is provided with an adjusting turntable, an L-shaped restraint plate is arranged on the surface of the adjusting turntable, an adjusting plate is fixedly connected to the top surface of the detection cavity, an air suction cavity is formed in the inside of the adjusting plate, a plurality of air vent holes are uniformly formed in the bottom surface of the air suction cavity, a multi-hole blocking plate is arranged on the bottom surface of the air suction cavity, an air outlet groove is formed in the upper surface of the adjusting turntable, an air inlet groove is formed in the bottom surface of the adjusting turntable, and a top closed pipe is arranged in the inside of the air outlet groove. The application realizes the control of the constant temperature and humidity environment and the automatic transfer of the test card through the circulation of the air circuit and the rotation and switching adjustment of the adjusting turntable.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fluorescence detection technology, in particular to a time-resolved fluorescence detection device and a detection method. BACKGROUND

[0002] The time-resolved fluorescence detection technology is widely used in biological, medical detection, environmental monitoring and other fields due to its high sensitivity, low background interference and other advantages.

[0003] The existing detection process usually includes sample pretreatment, test paper card sampling, constant temperature incubation and fluorescence signal reading. However, the existing technology usually detects in the laboratory, the environmental temperature is constant, the interference factors are small, for some scenes that need to be detected quickly, such as food detection, the process of sending for inspection is long, which is not conducive to improving the detection efficiency, the detection environment is complex and changeable, so that the detection result is greatly different from the laboratory detection result, the influence of the errors caused by manual operation on the detection result is easy to make the detection error larger. SUMMARY

[0004] The technical problem to be solved by the present application is to provide a time-resolved fluorescence detection device and a detection method, which realizes the control of constant temperature and humidity environment and the automatic transfer of test cards through the rotation switching regulation of gas circulation and the rotation of the turntable.

[0005] To solve the above technical problems, the technical scheme of the present application is as follows:

[0006] A time-resolved fluorescence detection device, comprising a detection box, the inside of the detection box is provided with a pipettor, a test tube rack, a test card, a two-axis moving table and a linear moving table, further comprising:

[0007] The inside of the detection box is provided with a detection cavity, the inside of the detection cavity is fixedly connected with an incubation box, the inside of the detection cavity is provided with an adjusting turntable, the surface of the adjusting turntable is symmetrically fixedly connected with an L-shaped restraint plate, the adjusting turntable is located in the relief groove opened on the surface of the incubation box, the inside top surface of the detection cavity is fixedly connected with an adjusting plate, the inside of the adjusting plate is provided with an air suction cavity, the air suction cavity is communicated with the inside of the air inlet of the air pump in the detection box through a pipeline, the inside bottom surface of the air suction cavity is uniformly provided with a plurality of air vent holes, the inside bottom surface of the air suction cavity is provided with a porous blocking plate, the upper surface of the adjusting turntable is provided with an air outlet groove, the bottom surface of the adjusting turntable is provided with an air inlet groove, the air outlet groove is communicated with the air outlet of the air pump arranged in the detection box through a pipeline, the inside of the air outlet groove is provided with a top closed pipe, the top closed pipe is located in the inside of the air suction cavity, the inside of the air suction cavity is provided with an L-shaped sliding rod, a cam and an arc-shaped blocking plate, the surface of the top closed pipe is provided with an air vent opening, the inner wall of the air suction cavity is fixedly connected with an arc-shaped blocking plate through an extension rod, the arc-shaped blocking plate is in abutment with the top closed pipe, the test card is transferred between the inside and the outside of the incubation box by rotating the adjusting turntable, when the test card is outside the incubation box, air flow is blown into the outside of the incubation box from the air inlet groove and enters the inside of the air suction cavity through the air vent holes, when the test card is inside the incubation box, air flow is blown into the inside of the incubation box from the air inlet groove and enters the inside of the air suction cavity through the air outlet groove, the top closed pipe and the air vent opening.

[0008] Further, the surface of the detection cavity is provided with a pulling sliding groove, the inside of the pulling sliding groove is slidingly connected with an L-shaped pulling plate, a plurality of T-shaped restraint blocks are arranged in parallel on the surface of the pulling sliding groove, the T-shaped restraint blocks are slidingly connected in the corresponding first sliding grooves opened on the bottom surface of the L-shaped pulling plate respectively, a C-shaped clamping table is fixedly connected on the surface of the L-shaped pulling plate, a first recess is opened in the C-shaped clamping table, a supporting block is inserted in the first recess, a first T-shaped column is arranged on the bottom surface of the supporting block near four corners, the first T-shaped column is slidingly connected in the corresponding second sliding groove opened on the bottom surface of the supporting block, a first insertion slot corresponding to the first T-shaped column is opened in the first recess of the C-shaped clamping table respectively, the first T-shaped column is inserted in the first insertion slot, a pipette is inserted in a T-shaped through groove opened on the surface of the supporting block, a push rod clamping head is clamped on the top of the piston push rod of the pipette, the two-axis moving table is installed on the inner wall of the detection cavity, the linear moving table is installed on the sliding table surface of the two-axis moving table, a butt joint block is fixedly connected on the surface of the linear moving table, an electromagnet is inlaid and installed in the inside of the butt joint block.

[0009] Further, the upper part of the supporting block is provided with a plurality of connecting seats, the upper part of the connecting seat is correspondingly provided with a connecting plug, the connecting seat is fixedly connected to the upper surface of the supporting block near the four corners, the surface of the connecting plug near the four corners of the supporting block is fixedly connected with a resisting rod, the connecting plug near the four corners of the supporting block is fixedly connected to the surface of the butt joint block, the surface of the butt joint block is provided with a cylindrical through hole, the surface center of the electromagnet is a cylindrical through groove, the inside of the cylindrical through hole is communicated with the inside of the cylindrical through groove, the supporting block is also fixedly connected to the upper surface of the push rod clamp, the connecting plug above the push rod clamp is located in the inside of the cylindrical through hole, the surface of the sliding table of the linear motion table is fixedly connected with an L-shaped connecting block, the L-shaped connecting block is fixedly connected with the connecting plug above the push rod clamp, a plurality of hooking blocks are arranged in an annular array in the inside of the connecting seat, the hooking blocks are respectively slidably connected in the first inclined groove arranged on the surface of the connecting seat, the surface of the hooking block is provided with a V-shaped groove, one face of the V-shaped groove is always vertically downward, and the surface of the connecting plug is provided with an annular groove.

[0010] Further, the surface of the push rod clamp is fixedly connected with an L-shaped plate, the lower part of the L-shaped plate is provided with an inclined block, the surface of the inclined block is symmetrically provided with a second T-shaped column, the second T-shaped column is respectively slidably connected in the corresponding third sliding groove arranged on the surface of the inclined block, the second T-shaped column is fixedly connected to the surface of the L-shaped plate, the surface of the second T-shaped column is sleeved with a first spring, and the two ends of the first spring are respectively fixedly connected with the inclined block and the L-shaped plate.

[0011] Further, the top closing pipe is rotatably connected in the third through groove arranged on the surface of the adjusting plate through a first bearing and a first dynamic seal, the cam is fixedly connected to the surface of the top closing pipe, the inside bottom surface of the air suction cavity is fixedly connected with a fixed constraint block, the L-shaped slide rod is slidably connected in the fourth through groove arranged on the surface of the fixed constraint block, the surface of the L-shaped slide rod is sleeved with a second spring, the two ends of the second spring are respectively fixedly connected with the fixed constraint block and the L-shaped slide rod, the top closing pipe is rotatably connected in the fifth through groove arranged on the surface of the incubation box through a second dynamic seal, the bottom surface of the adjusting plate is provided with a first motor, the end of the output shaft of the first motor is fixedly connected with a first bevel gear, the surface of the top closing pipe is fixedly connected with a second bevel gear, and the second bevel gear is meshedly connected with the first bevel gear.

[0012] Further, the surface of the L-shaped pull plate is provided with a transfer frame, a plurality of first magnetic blocks are inlaidly installed at the surface of the L-shaped pull plate and bonded with the transfer frame, a plurality of positioning heads are arranged at the surface of the L-shaped pull plate and bonded with the transfer frame, the positioning heads are slidingly connected in the fourth sliding grooves opened on the surface of the L-shaped pull plate, the bottom surface of the positioning head is provided with a third spring, and the two ends of the third spring are fixedly connected with the L-shaped pull plate and the positioning head respectively.

[0013] Further, the surface of the transfer frame is symmetrically provided with two groups of auxiliary heads, the auxiliary heads are slidingly connected in the corresponding fifth sliding grooves opened on the surface of the transfer frame, the surface of the auxiliary head is provided with a fourth spring, the two ends of the fourth spring are fixedly connected with the auxiliary head and the transfer frame respectively, and the surface of the L-shaped constraint plate is provided with fifth grooves corresponding to the two groups of auxiliary heads.

[0014] Further, the surface of the transfer frame is provided with a first placing groove, the test card is clamped in the first placing groove, the inside of the transfer frame is provided with a second placing groove, the inside of the second placing groove is slidingly connected with a top plate, the bottom surface of the top plate is fixedly connected with a plurality of positioning rods, the positioning rods are slidingly connected in the corresponding sixth through grooves opened in the inside of the second placing groove, the bottom surface of the top plate is fixedly connected with an adjusting top rod, and the adjusting top rod is slidingly connected in the seventh through groove opened on the surface of the second placing groove.

[0015] Further, the surface of the incubation box is inlaidly installed with a transparent window, the inside top surface of the incubation box is installed with a second motor, the end of the output shaft of the second motor is fixedly connected with a light shielding plate, the bottom surface of the light shielding plate is fixedly connected with a C-shaped frame, the inside bottom surface of the incubation box is hingedly connected with an adjusting lever, the surface of the C-shaped frame is provided with an inclined groove, and the inclined surface of the inclined groove is always bonded with the inclined surface of the bottom surface of the adjusting lever.

[0016] A time-resolved fluorescence detection method, specifically comprising the following steps:

[0017] Step one, the air pump inside the detection box pumps constant temperature and humidity air flow into the air inlet groove, the space outside the incubation box is heated to the set temperature, the air pump stops working, the L-shaped pull plate is pulled out of the inside of the detection box, the pipette is inserted into the inside of the supporting block, the push rod clamp is clamped on the top of the push rod of the pipette piston, the test tube containing the sample liquid to be tested is placed into the inside of the test tube rack, the test paper to be added is placed into the first placing groove, and the air pump continues to work.

[0018] Step two, after the L-shaped pull plate in step one is pushed into the detection box, the transfer frame carrying the test card is clamped between the two L-shaped constraint plates, and the auxiliary head is clamped into the corresponding fifth groove on the surface of the L-shaped constraint plate, respectively, after the sample liquid to be detected in the detection cavity, the pipette and the test card all reach the set temperature, the two-axis moving table and the linear moving table are controlled to move the butt joint block to the C-shaped clamping table, the electromagnet is energized, the magnetic force generated by the energized electromagnet moves the hooking block inside the connecting seat away from the connecting seat, when the connecting plug is inserted into the corresponding connecting seat, the vertical downward surface of the V-shaped groove of the hooking block is attached to the inner bottom surface of the surface annular groove of the corresponding connecting plug, at this time, the abutting rod abuts against the upper surface of the pipette head, through the cooperation of the two-axis moving table and the linear moving table, the pipette sucks the excess sample liquid, then moves to the test card, and drops an appropriate amount of sample liquid to the test card;

[0019] Step three, start the first motor, make the first motor drive the second bevel gear to rotate one hundred and eighty degrees through the first bevel gear and the second bevel gear, so that the transfer frame carrying the test card after dropping is rotated to the inside of the incubation box, at this time, the arc-shaped blocking plate inside the adjusting plate no longer blocks the ventilation opening, and under the extrusion of the cam, the second spring drives the multi-hole blocking plate to move to block the ventilation small hole, at this time, the air pump works to pump the constant temperature and humidity airflow from the air inlet groove, and the airflow returns to the air inlet of the air pump through the top closed pipe and the air suction cavity;

[0020] Step four, after the test card in the incubation box is incubated for a set time, the movement of the second motor drives the light shielding wiper plate to expose the transparent window, and the inclined groove extrudes the adjusting lever to make the adjusting lever push the adjusting rod, so that the test card moves to the transparent window under the action of the adjusting rod, and the fluorescence analyzer is started for detection.

[0021] The above-mentioned scheme of the present application at least includes the following beneficial effects:

[0022] The above-mentioned scheme of the present application realizes the flow direction adjustment of the airflow inside the detection cavity located in the incubation box through the rotation of the adjusting turntable, so that the device can simulate the preheating environment and the incubation environment;

[0023] Through the cooperation of the hooking block, the connecting plug, the electromagnet and the abutting rod, the quick fixing, releasing, liquid taking and dropping of the pipette are realized, and the manual operation error is reduced;

[0024] Through the rotation of the adjusting turntable, the cooperation of the transfer frame, the adjusting lever, the top plate, the adjusting rod, the second motor, the C-shaped frame and the transparent window, the test paper card is subjected to a relatively uniform constant temperature and humidity airflow during incubation, and is lifted to the transparent window during the detection stage, so as to improve the detection accuracy;

[0025] The detection temperature of the pipette, the test card and the sample liquid to be detected is kept constant by detecting the circulation of the airflow inside the box, and is not easily affected by the external environment. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 is a schematic diagram of the overall structure provided by the present application.

[0027] Figure 2 is a schematic diagram of the adjusting plate in the present application.

[0028] Figure 3 is a schematic diagram of the porous blocking plate in the present application.

[0029] Figure 4 is a schematic diagram of the air vent hole in the present application.

[0030] Figure 5 is a schematic diagram of the top closing tube in the present application.

[0031] Figure 6 is a schematic diagram of the light shielding wiping plate in the present application.

[0032] Figure 7 is a schematic diagram of the transfer frame in the present application.

[0033] Figure 8 is a schematic diagram of the supporting block in the present application.

[0034] Figure 9 is an enlarged view of A in the present application Figure 6 .

[0035] Figure 10 is an enlarged view of C in the present application Figure 7 .

[0036] Figure 11 is an enlarged view of B in the present application Figure 8 .

[0037] Figure 12 is an enlarged view of D in the present application Figure 4 .

[0038] Figure 13 is an enlarged view of E in the present application Figure 6 .

[0039] Figure 14 is a schematic diagram of the adjusting turntable in the present application.

[0040] In the figure: 101, detection box; 102, pipette; 103, test tube rack; 104, test card; 105, two-axis moving table; 106, linear moving table;

[0041] 201, detection cavity; 202, pull-out chute; 203, L-shaped pull-out plate; 204, T-shaped constraint block; 205, C-shaped clamping table; 206, supporting block; 207, connecting seat; 208, connecting plug; 209, abutting rod; 210, push rod clamping head; 211, hooking block; 212, first T-shaped column; 213, first slot; 214, L-shaped plate; 215, inclined block; 216, second T-shaped column; 217, first spring; 219, abutting block; 220, electromagnet; 221, cylindrical through hole; 222, L-shaped connecting block;

[0042] 301, adjusting turntable; 302, L-shaped constraint plate; 303, air outlet groove; 304, air inlet groove; 305, top closed pipe; 306, adjusting plate; 307, air suction cavity; 308, filtering block; 309, perforated blocking plate; 310, fixed constraint block; 311, L-shaped sliding rod; 312, cam; 313, arc-shaped blocking plate; 314, air passage opening; 315, second spring; 316, first motor; 317, first bevel gear; 318, second bevel gear; 319, air passage hole;

[0043] 401, transfer frame; 402, first magnetic block; 403, positioning head; 404, third spring;

[0044] 501, incubation box; 502, transparent window; 503, light shielding wiping plate; 504, second motor; 505, C-shaped frame; 506, first placement slot; 507, adjusting lever; 508, second placement slot; 509, top plate; 510, positioning rod; 511, adjusting top rod; 512, inclined slot; 513, auxiliary head; 514, fourth spring. DETAILED DESCRIPTION

[0045] Exemplary embodiments of the present disclosure will be described in greater detail below with reference to the accompanying drawings. Although exemplary embodiments of the present disclosure are shown in the drawings, it is understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure can be more thoroughly understood and so that the scope of the present disclosure can be completely conveyed to those skilled in the art.

[0046] As Figures 1 to 14 shown, an embodiment of the present application proposes a time-resolved fluorescence detection device, which comprises a detection box 101, the inside of the detection box 101 is provided with a pipette 102, a test tube rack 103, a test card 104, a two-axis moving table 105 and a linear moving table 106, and further comprises:

[0047] The inside of the detection box 101 is provided with a detection cavity 201, the inside of the detection cavity 201 is fixedly connected with an incubation box 501, the inside of the detection cavity 201 is provided with an adjusting turntable 301, the surface of the adjusting turntable 301 is fixedly connected with an L-shaped restraint plate 302 in a symmetrical mode, the adjusting turntable 301 is located in the relief groove formed on the surface of the incubation box 501, the inside top surface of the detection cavity 201 is fixedly connected with an adjusting plate 306, the inside of the adjusting plate 306 is provided with an air suction cavity 307, the air suction cavity 307 is connected with the inside of the air inlet of the air pump in the detection box 101 in a communicating mode through a pipeline, a plurality of air vent small holes 319 are uniformly formed in the inside bottom surface of the air suction cavity 307, the inside bottom surface of the air suction cavity 307 is provided with a multi-hole blocking plate 309, the upper surface of the adjusting turntable 301 is provided with an air outlet groove 303, the bottom surface of the adjusting turntable 301 is provided with an air inlet groove 304, the air outlet groove 303 is connected with the air outlet of the air pump arranged in the detection box 101 in a communicating mode through a pipeline, the inside of the air outlet groove 303 is provided with a top closed pipe 305, the top closed pipe 305 is located in the inside of the air suction cavity 307, the inside of the air suction cavity 307 is provided with an L-shaped sliding rod 311, a cam 312 and an arc-shaped blocking plate 313, the surface of the top closed pipe 305 is provided with an air vent opening 314, the inner wall of the air suction cavity 307 is fixedly connected with the arc-shaped blocking plate 313 through an extension rod, the arc-shaped blocking plate 313 is in abutment with the top closed pipe 305, the test card 104 is transferred between the inside and the outside of the incubation box 501 by rotating the adjusting turntable 301, when the test card 104 is outside the incubation box 501, the air flow is blown into the outside of the incubation box 501 from the air inlet groove 304 and enters the inside of the air suction cavity 307 through the air vent small holes 319, when the test card 104 is inside the incubation box 501, the air flow is blown into the inside of the incubation box 501 from the air inlet groove 304 and enters the inside of the air suction cavity 307 through the air outlet groove 303, the top closed pipe 305 and the air vent opening 314.

[0048] In the embodiment of the application, the inside of the detection box 101 is provided with a sound wave atomization device (not shown in the figure), an air pump (not shown in the figure), a dehumidification device (not shown in the figure), a heating device (not shown in the figure) and a refrigeration device (not shown in the figure), before detection, the air pump is used to make the gas circulate in the inside of the detection box 101, the sound wave atomization device, the heating device and the refrigeration device arranged on the air outlet side of the air pump are used to keep the gas entering the air inlet groove 304 at constant temperature and humidity.

[0049] Opening the L-shaped pull plate 203, the test card 104, the pipette 102 and the test tube rack 103 loaded with sample liquid test tubes are placed above the L-shaped pull plate 203, the L-shaped pull plate 203 is closed, the constant temperature and humidity air flow of the set temperature and humidity is pumped into the air inlet groove 304 at the bottom of the adjusting turntable 301, the area outside the incubation box 501 in the detection cavity 201 is warmed to the set value, so as to preheat the sample liquid, the test card 104 and the pipette 102;

[0050] When the temperature of the sample liquid, the test card 104 and the pipette 102 reaches the set value measured by the temperature sensor (not shown in the figure) installed in the detection cavity 201, the two-axis moving table 105 and the linear moving table 106 are used to move the pipette 102 above the test tube loaded with the sample liquid to be tested in the test tube rack 103, and the center axis of the pipette 102 drop adding port and the center axis of the test tube are overlapped, at this time the pipette 102 is dropped to the set depth in the test tube by the two-axis moving table 105, and the piston push rod of the pipette 102 is moved upward by the linear moving table 106, so as to extract the excess sample liquid to be tested; the pipette 102 is moved to the liquid adding port of the test card 104 by the two-axis moving table 105, and the center axis of the pipette 102 drop adding port and the center axis of the test card 104 liquid adding port are overlapped, the piston push rod of the pipette 102 is pressed downward by the linear moving table 106, and after adding the appropriate amount of sample liquid to be tested, the adjusting turntable 301 is rotated to transfer the test card 104 after drop adding to the inside of the incubation box 501 for incubation;

[0051] It should be noted that the connection mode of the pipeline in the embodiment is to open a reserved hole, and the end of the pipeline is fixed in the reserved hole, the installation path of the pipeline can be realized by the reserved hole slot, which is known in the prior art, and will not be described in detail here. The working principle and use mode of the wave atomization device, the air pump, the dehumidification device, the heating device and the refrigeration device are known in the prior art, and will not be described in detail here.

[0052] The slide table of the two-axis moving table 105 can move in a vertical plane parallel to the sliding direction of the L-shaped pull plate 203, and the slide table of the linear moving table 106 can move in the vertical direction. The working principle and use process of the two-axis moving table 105 and the linear moving table 106 are known in the prior art, and will not be described in detail here.

[0053] The inside of the detection box 101 is also provided with a fluorescence analyzer, which detects the test paper card after incubation through the transparent window 502. The fluorescence analyzer is installed above the incubation box 501, and the working principle and use process are known in the prior art, and will not be described in detail here.

[0054] The center axes of the liquid inlet of the test card 104, the test tubes in the test tube rack 103 and the bottom drop inlet of the pipette 102 are in the same plane, facilitating the movement of the two-axis moving table 105 and the linear moving table 106.

[0055] The surface of the detection cavity 201 is provided with a pull-out sliding groove 202. The inside of the pull-out sliding groove 202 is slidably connected with an L-shaped pull-out plate 203. The surface of the pull-out sliding groove 202 is provided in parallel with a plurality of T-shaped restraint blocks 204. The T-shaped restraint blocks 204 are respectively slidably connected in the corresponding first sliding grooves opened on the bottom surface of the L-shaped pull-out plate 203. The surface of the L-shaped pull-out plate 203 is fixedly connected with a C-shaped clamping table 205. The surface of the C-shaped clamping table 205 is provided with a first recess into which a supporting block 206 is inserted. The bottom surface of the supporting block 206 is provided near the four corners with first T-shaped columns 212 which are slidably connected in the corresponding second sliding grooves opened on the bottom surface of the supporting block 206. The first recess opened on the surface of the C-shaped clamping table 205 is respectively provided with first insertion slots 213 corresponding to the first T-shaped columns 212. The first T-shaped columns 212 are inserted into the corresponding first insertion slots 213. The pipette 102 is inserted into the T-shaped through groove opened on the surface of the supporting block 206. The top of the piston push rod of the pipette 102 is clamped with a push rod clamping head 210. The two-axis moving table 105 is installed on the inner wall of the detection cavity 201. The linear moving table 106 is installed on the sliding table surface of the two-axis moving table 105. The surface of the linear moving table 106 is fixedly connected with a butt joint block 219. The butt joint block 219 is inlaidly installed with an electromagnet 220.

[0056] The top of the supporting block 206 is provided with a plurality of connecting seats 207. The top of each connecting seat 207 is correspondingly provided with a connecting plug 208. The connecting seats 207 are respectively fixedly connected on the top surface of the supporting block 206 near the four corners. The surface of the connecting plug 208 near the four corners of the supporting block 206 is fixedly connected with a resisting rod 209. The connecting plug 208 near the four corners of the supporting block 206 is fixedly connected on the surface of the butt joint block 219. The surface of the butt joint block 219 is provided with a cylindrical through hole 221. The surface of the electromagnet 220 is a columnar through groove. The inside of the cylindrical through hole 221 is in communication with the inside of the columnar through groove. The supporting block 206 is further fixedly connected on the top surface of the push rod clamping head 210. The connecting plug 208 above the push rod clamping head 210 is located in the inside of the cylindrical through hole 221. The sliding table surface of the linear moving table 106 is fixedly connected with an L-shaped connecting block 222. The L-shaped connecting block 222 is fixedly connected with the connecting plug 208 above the push rod clamping head 210. A plurality of hooking blocks 211 are annularly arranged in the inside of the connecting seat 207. The hooking blocks 211 are respectively slidably connected in the first inclined surface grooves opened on the surface of the connecting seat 207. The surface of the hooking block 211 is provided with a V-shaped recess. One face of the V-shaped recess is always vertically downwardly arranged. The surface of the connecting plug 208 is provided with an annular groove.

[0057] In the embodiment of the present application, the T-shaped constraint block 204 makes the L-shaped pull plate 203 not easy to be separated from the detection box 101. When placing the pipette 102, first, the pipette 102 is inserted into the inside of the supporting block 206, the push rod clamp head 210 is inserted into the end of the piston push rod of the pipette 102, the supporting block 206 is inserted into the first groove opened on the surface of the C-shaped clamping table 205, the first T-shaped column 212 in the inside of the supporting block 206 is inserted into the inside of the first insertion slot 213 under the action of gravity. At this time, when the L-shaped pull plate 203 moves relative to the detection box 101, the cooperation of the first T-shaped column 212 and the first insertion slot 213 makes the supporting block 206 not easy to move horizontally relative to the C-shaped clamping table 205. The electromagnet 220 is energized, the hooking block 211 moves to the top of the first inclined groove under the magnetic force of the electromagnet 220, and the V-shaped groove of the hooking block 211 is exposed outside the first inclined groove. The downward movement of the butt joint block 219 is driven by the two-axis moving table 105 and the linear moving table 106, so that the connecting plug 208 is inserted into the inside of the corresponding connecting seat 207. During the downward movement, the connecting plug 208 first extrudes the hooking block 211, so that the hooking block 211 is extruded into the first inclined groove against the magnetic attraction force of the electromagnet 220, until the hooking block 211 is inserted into the annular groove of the connecting plug 208. At this time, the hooking of the V-shaped groove of the hooking block 211 and the annular groove makes the connecting plug 208 unable to be pulled out of the inside of the connecting seat 207. The function of the abutting rod 209 is that when the push rod clamp head 210 is lifted upward by the linear moving table 106 under the cooperation of the connecting seat 207, the connecting plug 208 and the L-shaped connecting block 222 on the surface of the push rod clamp head 210, the abutting rod 209 abuts against the surface of the pipette head of the pipette 102, so as to prevent the pipe body of the pipette 102 from being lifted upward under the resistance of the piston push rod.

[0058] The energized operation of the electromagnet 220 also makes the first T-shaped column 212 in the inside of the supporting block 206 move upward under the magnetic force, so as to leave the inside of the first insertion slot 213. At this time, the linear moving table 106, the butt joint block 219, the connecting plug 208, the connecting seat 207, the supporting block 206 and the pipette 102 can be directly horizontally moved by the two-axis moving table 105.

[0059] The surface of the push rod clamp head 210 is fixedly connected with an L-shaped plate 214. An inclined block 215 is arranged below the L-shaped plate 214. Second T-shaped columns 216 are symmetrically arranged on the surface of the inclined block 215. The second T-shaped columns 216 are respectively and slidingly connected in corresponding third sliding grooves opened on the surface of the inclined block 215. The second T-shaped columns 216 are all fixedly connected on the surface of the L-shaped plate 214. First springs 217 are all sleeved on the surfaces of the second T-shaped columns 216. The two ends of each first spring 217 are fixedly connected with the inclined block 215 and the L-shaped plate 214.

[0060] In the embodiment of the present application, during the process of clamping the push rod clamp 210 into the top of the piston push rod of the pipette 102, the inclined block 215 is first pressed by the top of the piston push rod, so that the inclined block 215 is moved upward along the surface of the second T-shaped column 216 against the elastic potential energy of the first spring 217, and when the top of the piston push rod of the pipette 102 is completely inserted into the interior of the push rod clamp 210, the inclined block 215 is reset under the action of the elastic potential energy of the first spring 217 and abuts against the piston push rod, so that the push rod clamp 210 is kept stationary relative to the piston push rod, thereby facilitating the plug-in connection of the connecting seat 207 on the surface of the push rod clamp 210 and the corresponding connecting plug 208.

[0061] The top closing pipe 305 is rotatably connected in the third through groove on the surface of the adjusting plate 306 through a first bearing and a first dynamic seal, the cam 312 is fixedly connected on the surface of the top closing pipe 305, the fixed constraint block 310 is fixedly connected on the inner bottom surface of the suction cavity 307, the L-shaped slide rod 311 is slidably connected in the fourth through groove on the surface of the fixed constraint block 310, the second spring 315 is sleeved on the surface of the L-shaped slide rod 311, and two ends of the second spring 315 are fixedly connected with the fixed constraint block 310 and the L-shaped slide rod 311 respectively, the top closing pipe 305 is rotatably connected in the fifth through groove on the surface of the incubation box 501 through a second dynamic seal, the first motor 316 is installed on the bottom surface of the adjusting plate 306, the first bevel gear 317 is fixedly connected on the end of the output shaft of the first motor 316, and the second bevel gear 318 is fixedly connected on the surface of the top closing pipe 305 and is in meshing connection with the first bevel gear 317.

[0062] In the embodiment of the present application, when the test card 104 is located outside the incubation box 501, the airflow is blown from the air inlet groove 304 into the detection cavity 201 and into the suction cavity 307 from the air vent hole 319, and then is returned to the air inlet of the air pump through the pipeline, the filter block 308 can be installed in the suction cavity 307, so that the airflow entering the suction cavity 307 is filtered by the filter block 308 and then returned to the air inlet of the air pump through the pipeline, and at this time, the air vent opening 314 on the surface of the top closing pipe 305 is blocked by the arc-shaped blocking plate 313.

[0063] When the test card 104 enters the interior of the incubation box 501 by adjusting the rotation of the rotation table 301 by 180 degrees, the top closing pipe 305 rotates by 180 degrees so that the ventilation opening 314 is no longer blocked by the arc-shaped blocking plate 313, and the cam 312 extrudes the L-shaped slide rod 311, which moves in the interior of the fixed constraint block 310 to the direction of stretching the second spring 315, and drives the perforated blocking plate 309 to block all the ventilation holes 319, at this time, the air pump pumps air into the interior of the incubation box 501 through the air inlet groove 304, and then enters the interior of the suction cavity 307 through the air outlet groove 303, the top closing pipe 305 and the ventilation opening 314, and returns to the air inlet of the air pump through the pipeline.

[0064] The adjustment rotation table 301 rotates by 180 degrees in the following manner: the first motor 316 is powered to operate, the operation of the first motor 316 drives the first bevel gear 317 to rotate the second bevel gear 318, and the rotation of the second bevel gear 318 synchronously drives the top closing pipe 305 to rotate.

[0065] The surface of the L-shaped pull-out plate 203 is provided with a transfer frame 401, a plurality of first magnetic blocks 402 are inlaidly installed at the surface of the L-shaped pull-out plate 203 and the transfer frame 401, a plurality of positioning heads 403 are arranged at the surface of the L-shaped pull-out plate 203 and the transfer frame 401, the positioning heads 403 are slidingly connected in fourth sliding grooves formed in the surface of the L-shaped pull-out plate 203, the bottom surface of the positioning head 403 is provided with a third spring 404, and the two ends of the third spring 404 are fixedly connected with the L-shaped pull-out plate 203 and the positioning head 403 respectively, and the bottom surface of the transfer frame 401 is provided with fourth grooves matched with the positioning heads 403.

[0066] In the embodiment of the present application, the cross section of the first magnetic block 402 is a quarter circle, the transfer frame 401 is kept relatively fixed with the L-shaped pull-out plate 203 through the magnetic attraction of the first magnetic block 402 and the fourth grooves corresponding to the bottom surface of the transfer frame 401 and the positioning head 403, the vertical surface of the positioning head 403 is directed in the same direction as the L-shaped pull-out plate 203 is separated from the detection box 101, when the L-shaped pull-out plate 203 drives the transfer frame 401 to enter the interior of the detection box 101 and makes the transfer frame 401 located between the two L-shaped constraint plates 302, when the adjustment rotation table 301 rotates, the L-shaped constraint plate 302 drives the transfer frame 401 to overcome the magnetic attraction of the first magnetic block 402, and makes the fourth groove extrude the curved surface of the positioning head 403, so that the positioning head 403 overcomes the elastic potential energy of the third spring 404 and slides into the fourth sliding groove formed in the surface of the L-shaped pull-out plate 203, at this time, the transfer frame 401 is driven by the rotation of the adjustment rotation table 301 and is transferred to the interior of the incubation box 501.

[0067] The surface of the transfer frame 401 is symmetrically provided with two groups of auxiliary heads 513, the auxiliary heads 513 are respectively slidably connected in the corresponding fifth sliding grooves opened on the surface of the transfer frame 401, the surface of the auxiliary head 513 is provided with the fourth spring 514, the two ends of the fourth spring 514 are respectively fixedly connected with the auxiliary head 513 and the transfer frame 401, and the surface of the L-shaped constraint plate 302 is respectively provided with the fifth recess corresponding to the two groups of auxiliary heads 513.

[0068] In the embodiment of the application, the auxiliary head 513 is inserted into the fifth recess under the elastic potential of the fourth spring 514, so that the transfer frame 401 keeps relative static with the adjusting turntable 301 during following the rotation of the adjusting turntable 301.

[0069] The surface of the transfer frame 401 is provided with the first placing groove 506, the test card 104 is clamped in the first placing groove 506, the inside of the transfer frame 401 is provided with the second placing groove 508, the inside of the second placing groove 508 is slidably connected with the top plate 509, the bottom surface of the top plate 509 is fixedly connected with the positioning rods 510, the positioning rods 510 are respectively slidably connected in the corresponding sixth through grooves opened in the second placing groove 508, the bottom surface of the top plate 509 is fixedly connected with the adjusting top rod 511, and the adjusting top rod 511 is slidably connected in the seventh through groove opened on the surface of the second placing groove 508.

[0070] In the embodiment of the application, when the test card 104 is incubated in the incubator 501 and the incubation is completed, the top plate 509 is moved upward under the constraint of the positioning rods 510 and the sixth through grooves, and the test card 104 is pushed out of the first placing groove 506 until the test card 104 abuts against the transparent window 502, so as to facilitate the detection of the fluorescence detector.

[0071] The surface of the incubator 501 is embeddedly installed with the transparent window 502, the inside top surface of the incubator 501 is installed with the second motor 504, the end of the output shaft of the second motor 504 is fixedly connected with the light shielding plate 503, the bottom surface of the light shielding plate 503 is fixedly connected with the C-shaped frame 505, the inside bottom surface of the incubator 501 is hingedly connected with the adjusting lever 507, the surface of the C-shaped frame 505 is provided with the inclined groove 512, and the inclined surface of the inclined groove 512 always abuts against the inclined surface of the bottom surface of the adjusting lever 507.

[0072] In the embodiment of the present application, when the light-shielding wiper 503 covers the transparent window 502, the upper surface of the adjusting lever 507 remains horizontal and is below the bottom surface of the adjusting turntable 301. During the process of retracting the light-shielding wiper 503 to expose the transparent window 502 by the second motor 504, the C-shaped frame 505 at the bottom of the light-shielding wiper 503 cooperates with the inclined surface at the bottom of the adjusting lever 507 through the inclined groove 512, so that the adjusting lever 507 rotates relative to the incubation box 501, and the top plate 509 is lifted by the adjusting top rod 511, thereby moving the test card 104 upward.

[0073] During the movement of the light-shielding wiper 503, the transparent window 502 is cleaned to reduce detection errors.

[0074] A time-resolved fluorescence detection method, specifically comprising the following steps:

[0075] Step one, the air pump inside the detection box 101 pumps constant temperature and humidity air flow into the air inlet groove 304, the space outside the incubation box 501 is heated to a set temperature, then the air pump stops working, the L-shaped pull plate 203 is pulled out of the inside of the detection box 101, the pipette 102 is inserted into the inside of the supporting block 206, the push rod clamp 210 is clamped on the top of the piston push rod of the pipette 102, the test tube containing the sample liquid to be tested is placed in the test tube rack 103, and then the test paper card to be added is placed in the first placing groove 506, and the air pump continues to work.

[0076] Step two, after the L-shaped pull plate 203 in step one is pushed into the inside of the detection box 101, the transfer frame 401 carrying the test card 104 is clamped between the two L-shaped constraint plates 302, and the auxiliary head 513 is respectively clamped into the fifth groove on the surface of the L-shaped constraint plate 302. After the sample liquid in the detection cavity 201, the pipette 102 and the test card 104 reach the set temperature, the two-axis moving table 105 and the linear moving table 106 move the butt joint block 219 to the C-shaped clamping table 205, the electromagnet 220 is energized, the magnetic force generated by the energized electromagnet 220 makes the hooking block 211 move in the inside of the connecting seat 207 away from the connecting seat 207, when the connecting plug 208 is inserted into the corresponding connecting seat 207, the vertical downward surface of the V-shaped groove of the hooking block 211 is matched with the inner bottom surface of the annular groove on the surface of the corresponding connecting plug 208, at this time, the abutting rod 209 abuts against the upper surface of the pipetting head of the pipette 102, through the cooperation of the two-axis moving table 105 and the linear moving table 106, the pipette 102 takes excess sample liquid, then moves to the test card 104, and drops an appropriate amount of sample liquid to the test card 104.

[0077] Step three, start the first motor 316, make the first motor 316 drive the second bevel gear 318 to rotate 180 degrees through the first bevel gear 317 and the second bevel gear 318, so that the transfer frame 401 carrying the test card 104 after dropwise addition is rotated to the inside of the incubation box 501, at this time the arc-shaped baffle 313 inside the adjusting plate 306 no longer blocks the ventilation opening 314, at the same time under the extrusion of the cam 312, the second spring 315 drives the multi-hole baffle 309 to move to block the ventilation small hole 319, at this time the air pump works to pump the constant temperature and humidity airflow from the air inlet groove 304, and the airflow flows back to the air inlet of the air pump from the air outlet groove 303 through the top closed pipe 305 and the air suction cavity 307;

[0078] Step four, when the test card 104 in the incubation box 501 is incubated for a set time, the movement of the light shielding baffle 503 driven by the second motor 504 exposes the transparent window 502, and the inclined groove 512 extrudes the adjusting rod 507 to make the adjusting rod 507 push the adjusting rod 511, so that the test card 104 is moved to be attached to the transparent window 502 under the action of the adjusting rod 511, and the fluorescence analyzer is started for detection.

[0079] It should be noted that the air pump is connected with the inside of the air inlet groove 304 through a pipeline, the end of the pipeline is rotatably connected to the inside of the air inlet groove 304 through a third dynamic seal and a third bearing, so as to avoid movement interference between the adjusting turntable 301 and the pipeline during rotation.

[0080] The above is the preferred embodiment of the present application, it should be pointed out that for ordinary skilled in the art, without departing from the principles of the present application, can make a number of improvements and refinements, these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A time-resolved fluorescence detection apparatus comprising a detection box, the inside of the detection box being provided with a pipette, a test tube rack, a test card, a two-axis moving table, and a linear moving table, characterized in that, The inside of the detection box is provided with a detection cavity, the inside of the detection cavity is fixedly connected with an incubation box, the inside of the detection cavity is provided with an adjusting turntable, the surface of the adjusting turntable is symmetrically fixedly connected with an L-shaped restraint plate, the adjusting turntable is located in the relief groove opened on the surface of the incubation box, the inside of the detection cavity is fixedly connected with an adjusting plate, the inside of the adjusting plate is provided with an air suction cavity, the air suction cavity is communicated with the inside of the air inlet of the air pump in the detection box through a pipeline, a plurality of air vent holes are uniformly arranged on the inside bottom surface of the air suction cavity, a porous blocking plate is arranged on the inside bottom surface of the air suction cavity, an air outlet groove is arranged on the upper surface of the adjusting turntable, an air inlet groove is arranged on the bottom surface of the adjusting turntable, the air outlet groove is communicated with the air outlet of the air pump arranged in the detection box through a pipeline, a top closing pipe is arranged in the air suction cavity, an L-shaped sliding rod, a cam and an arc-shaped blocking plate are arranged in the air suction cavity, a ventilation opening is arranged on the surface of the top closing pipe, the inner wall of the air suction cavity is fixedly connected with an arc-shaped blocking plate through an extension rod, the arc-shaped blocking plate is in abutment with the top closing pipe, the top closing pipe is rotatably connected in the third through groove opened on the surface of the adjusting plate through a first bearing and a first dynamic seal, the cam is fixedly connected to the surface of the top closing pipe, a fixed restraint block is fixedly connected to the inside bottom surface of the air suction cavity, the L-shaped sliding rod is slidably connected in the fourth through groove opened on the surface of the fixed restraint block, a second spring is sleeved on the surface of the L-shaped sliding rod, and the two ends of the second spring are fixedly connected with the fixed restraint block and the L-shaped sliding rod respectively, the top closing pipe is rotatably connected in the fifth through groove opened on the surface of the incubation box through a second dynamic seal, a first motor is installed on the bottom surface of the adjusting plate, a first bevel gear is fixedly connected to the end of the output shaft of the first motor, a second bevel gear is fixedly connected to the surface of the top closing pipe, the second bevel gear is meshingly connected with the first bevel gear, a two-axis moving table is installed on the inner wall of the detection cavity, a linear moving table is installed on the sliding table surface of the two-axis moving table, a butt joint block is fixedly connected to the surface of the linear moving table, and an electromagnet is inlaidly installed in the inside of the butt joint block, the test card is transferred between the inside and outside of the incubation box through the rotation of the adjusting turntable, when the test card is outside the incubation box, the air flow is blown into the outside of the incubation box from the air inlet groove and enters the inside of the air suction cavity through the air vent holes, when the test card is in the incubation box, the air flow is blown into the inside of the incubation box from the air inlet groove and enters the inside of the air suction cavity through the air outlet groove, the top closing pipe and the ventilation opening.

2. The time resolved fluorescence detection device according to claim 1, characterized in that The surface of the detection cavity is provided with a pull-out sliding groove, the inside of the pull-out sliding groove is slidably connected with an L-shaped pull-out plate, the surface of the pull-out sliding groove is provided in parallel with a plurality of T-shaped constraint blocks, the T-shaped constraint blocks are slidably connected in corresponding first sliding grooves opened on the bottom surface of the L-shaped pull-out plate respectively, the surface of the L-shaped pull-out plate is fixedly connected with a C-shaped clamping table, a supporting block is inserted in a first recess groove opened on the surface of the C-shaped clamping table, the bottom surface of the supporting block is provided with a first T-shaped column near each corner, the first T-shaped column is slidably connected in a corresponding second sliding groove opened on the bottom surface of the supporting block, a first insertion slot corresponding to the first T-shaped column is respectively opened in the first recess groove on the surface of the C-shaped clamping table, the first T-shaped column is inserted in the inside of the corresponding first insertion slot, a pipette is inserted in a T-shaped through groove opened on the surface of the supporting block, and a push rod clamp head is clamped on the top of a push rod of the pipette.

3. The time resolved fluorescence detection device according to claim 2, characterized in that The upper part of the supporting block is provided with a plurality of connecting seats, the upper part of each connecting seat is provided with a connecting plug correspondingly, the connecting seat is fixedly connected on the upper surface of the supporting block near each corner respectively, the surface of the connecting plug near each corner of the supporting block is fixedly connected with a contact rod, the connecting plug near each corner of the supporting block is fixedly connected on the surface of the butt joint block, the surface of the butt joint block is provided with a cylindrical through hole, the surface of the electromagnet is a cylindrical through groove at the center position, the inside of the cylindrical through hole is communicated with the inside of the cylindrical through groove, the supporting block is further fixedly connected on the upper surface of the push rod clamp head, the connecting plug above the push rod clamp head is located in the inside of the cylindrical through hole, the sliding table surface of the linear moving table is fixedly connected with an L-shaped connecting block, the L-shaped connecting block is fixedly connected with the connecting plug above the push rod clamp head, a plurality of hooking blocks are arranged in an annular array in the inside of the connecting seat, the hooking blocks are slidably connected in first inclined groove surfaces opened on the surface of the connecting seat respectively, the surface of the hooking block is provided with a V-shaped groove, one face of the V-shaped groove is always arranged vertically downward, and the surface of the connecting plug is provided with an annular groove.

4. The time resolved fluorescence detection device according to claim 3, characterized in that The surface of the push rod clamp head is fixedly connected with an L-shaped plate, the lower part of the L-shaped plate is provided with an inclined block, the surface of the inclined block is provided with a second T-shaped column symmetrically, the second T-shaped column is slidably connected in corresponding third sliding grooves opened on the surface of the inclined block respectively, the second T-shaped column is fixedly connected on the surface of the L-shaped plate, and the surface of the second T-shaped column is sleeved with a first spring.

5. The time resolved fluorescence detection apparatus of claim 2, wherein, The surface of the L-shaped pull plate is provided with a transfer frame, a plurality of first magnetic blocks are inlaidly installed at the surface of the L-shaped pull plate and bonded with the transfer frame, a plurality of positioning heads are arranged at the surface of the L-shaped pull plate and bonded with the transfer frame, the positioning heads are slidingly connected in the fourth sliding grooves opened on the surface of the L-shaped pull plate, the bottom surface of the positioning head is provided with a third spring, and the two ends of the third spring are fixedly connected with the L-shaped pull plate and the positioning head respectively.

6. The time resolved fluorescence detection device according to claim 5, characterized in that The surface of the transfer frame is symmetrically provided with two groups of auxiliary heads, the auxiliary heads are slidingly connected in the corresponding fifth sliding grooves opened on the surface of the transfer frame respectively, the surface of the auxiliary head is provided with a fourth spring, and the two ends of the fourth spring are fixedly connected with the auxiliary head and the transfer frame respectively.

7. The time resolved fluorescence detection device of claim 5, wherein, The surface of the transfer frame is provided with a first placing groove, the test card is clamped in the first placing groove, the inside of the transfer frame is provided with a second placing groove, the inside of the second placing groove is slidingly connected with a top plate, the bottom surface of the top plate is fixedly connected with a plurality of positioning rods, the positioning rods are slidingly connected in the corresponding sixth through grooves opened in the inside of the second placing groove respectively, the bottom surface of the top plate is fixedly connected with an adjusting top rod, and the adjusting top rod is slidingly connected in the seventh through groove opened on the surface of the second placing groove.

8. The time resolved fluorescence detection device according to claim 7, characterized in that The surface of the incubation box is inlaidly installed with a transparent window, the inside top surface of the incubation box is installed with a second motor, the end of the output shaft of the second motor is fixedly connected with a light shielding plate, the bottom surface of the light shielding plate is fixedly connected with a C-shaped frame, the inside bottom surface of the incubation box is hingedly connected with an adjusting lever, the surface of the C-shaped frame is provided with an inclined groove, and the inclined surface of the inclined groove is always bonded with the inclined surface of the bottom surface of the adjusting lever.

9. A time resolved fluorescence detection method for use in the apparatus of claim 8, characterized by, Specifically includes the following steps: Step one, make the air pump in the detection box pump the constant temperature and humidity airflow into the air inlet groove, make the space temperature outside the incubation box rise to the set temperature, stop the air pump, pull out the L-shaped pull plate from the inside of the detection box, insert the pipette into the inside of the supporting block, clamp the push rod clamp on the top of the pipette piston push rod, put the test tube carrying the sample liquid into the inside of the test tube rack, and continue to work the air pump after putting the test paper to be added into the first placing groove. Step two, after the L-shaped pull plate in step one is pushed into the detection box, the transfer frame carrying the test card is clamped between the two L-shaped constraint plates, and the auxiliary head is clamped into the corresponding fifth groove on the surface of the L-shaped constraint plate, respectively, after the sample liquid to be tested in the detection cavity, the pipette and the test card all reach the set temperature, the control two-axis moving table and the linear moving table move the butt joint block to the C-shaped card table, the electromagnet is energized, the magnetic force generated by the energized electromagnet makes the hook block move in the inside of the connecting seat to the direction away from the connecting seat, when the connecting plug is inserted into the corresponding connecting seat, the vertical downward surface of the V-shaped groove of the hook block is in contact with the inner bottom surface of the surface ring groove of the corresponding connecting plug, at this time the abutting rod is in contact with the upper surface of the pipette head, through the cooperation of the two-axis moving table and the linear moving table, the pipette takes the excess sample liquid, then moves to the test card, and drops the appropriate amount of sample liquid to the test card; Step three, start the first motor, make the first motor drive the second bevel gear to rotate one hundred and eighty degrees through the first bevel gear and the second bevel gear, so that the transfer frame carrying the test card after dropping is rotated to the inside of the incubation box, at this time the arc-shaped baffle plate inside the adjusting plate no longer blocks the ventilation opening, and under the extrusion of the cam, the second spring drives the multi-hole baffle plate to move to block the ventilation small hole, at this time the air pump works to make the constant temperature and humidity air flow pumped out from the air inlet groove, and backflow to the air inlet of the air pump from the air outlet groove through the top closed pipe and the air suction cavity; Step four, after the test card in the incubation box is incubated for a set time, the movement of the light shielding wiper plate is driven by the second motor, so that the transparent window is exposed, and the adjusting lever is extruded by the inclined groove, so that the adjusting lever drives the adjusting rod, and the test card moves to the transparent window under the action of the adjusting rod, and the fluorescence analyzer is started for detection.

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