Preparation method of lecithin emulsifier applicable to acid environment and application of lecithin emulsifier in cat hair formation
A composite emulsifier prepared by high-pressure homogenization of lecithin and Anemarrhena asphodeloides extract solves the problem of decreased emulsification ability of lecithin in acidic environments, achieving effective emulsification and dispersion in cat hair removal, and promoting the softening and removal of cat hair.
Patent Information
- Application Number
- CN202511260588.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-04
- Publication Date
- 2025-12-12
AI Technical Summary
In acidic environments, the emulsifying ability of lecithin emulsifiers decreases significantly, affecting their effectiveness in cat hair removal, and existing technologies have failed to effectively solve this problem.
A novel lecithin emulsifier was prepared by high-pressure homogenization of lecithin and Anemarrhena asphodeloides extract. The steroidal saponins in Anemarrhena asphodeloides combine with lecithin to form a stable composite emulsifier, which maintains emulsification ability and improves dispersibility.
In an acidic environment, the newly prepared lecithin emulsifier significantly improved emulsification stability and dispersibility, promoted the softening and expulsion of cat hair from the cat's stomach, reduced the strength of the cat hair structure, increased the contact rate between cat gastric juice and cat hair, and promoted the degradation of cat hair.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of emulsifier preparation, in particular to a preparation method of lecithin emulsifier capable of maintaining emulsifying ability in an acidic environment and application thereof in cat dehairing. BACKGROUND
[0002] Lecithin, i.e. phosphatidylcholine, is composed of four parts, i.e. a phosphate group, a choline group, glycerol and two fatty acid chains, and has both hydrophilicity (hydrophilic choline group and phosphate group) and lipophilicity (hydrophobic fatty acid), and is an excellent zwitterionic emulsifier. The groups that can be charged are the phosphate group (negative charge group) and the choline group (positive charge group). In different pH environments, the choline group (quaternary ammonium salt cation) has permanent positive charge and is not affected by the pH value of the solution, while the phosphate group is negatively charged in neutral and weakly alkaline environments, and is not charged in acidic environments. Therefore, in neutral and weakly alkaline environments, the net charge of the hydrophilic head of lecithin is close to zero, and when lecithin molecules are closely arranged at the oil / water interface, the electrostatic repulsion between the hydrophilic heads is small, the lecithin is closely and stably arranged, and can well exert the emulsifying performance; while in acidic environments, the hydrophilic head of lecithin is positively charged, and when lecithin molecules are closely arranged at the oil / water interface, the electrostatic repulsion between the hydrophilic heads is large, resulting in that the lecithin is not closely and stably arranged, and thus the stability of the oil / water interface is destroyed, which shows that the emulsifying ability of lecithin in acidic environments is reduced. Therefore, the high positive charge of lecithin in acidic environments is the key to affect its emulsifying ability.
[0003] At present, the methods for improving the emulsifying ability of lecithin mainly focus on preparing composite emulsifiers by compounding lecithin with other substances (Effect of interfacial synergism between gliadin particles and soybean lecithin on W1 / O / W2 emulsions: Encapsulation stability, interfacial rheology, nutrition delivery and in vitro digestion, Zhang X; Lecithin products to meet the needs of food processing, Di Jile), and no solution is given to the problem of high positive charge of lecithin in acidic environments. Anemarrhena asphodeloides contains various components, such as saponins, polysaccharides and flavonoids, etc. At present, there is no report on the use of Anemarrhena asphodeloides to reduce the proportion of positive charge of lecithin in acidic environments and improve the emulsifying ability of lecithin in acidic environments.
[0004] Cats like to clean and comb themselves by licking their fur, and a large amount of cat hair is ingested at the same time. Long hair that cannot be quickly degraded is prone to accumulate in the cat's stomach in the form of hair balls, which are difficult to discharge. When the number and size of hair balls are too large, it can cause diseases such as hair ball syndrome and intestinal obstruction, and even death. Therefore, the degradation and softening of cat hair (cat hair degradation) is of great significance to the health of cats. The gastric acid and pepsin in the cat's stomach can destroy the structure of cat hair and make it degrade and soften. However, there is a lipid layer on the surface of cat hair, which is secreted by the sebaceous glands of the cat itself, and plays a role in smoothing and protecting the structure of cat hair. However, this layer of lipid will hinder the full contact of gastric acid and pepsin with the protein structure of cat hair, thereby reducing the degradation and softening efficiency of cat hair in the cat's stomach. Currently, there is no report on the application of lecithin emulsifier with emulsifying and degreasing ability in an acidic environment to cat hair degradation. SUMMARY
[0005] The purpose of the present application is to provide a preparation method of lecithin emulsifier capable of maintaining emulsifying ability in an acidic environment and its application in cat hair degradation.
[0006] In a first aspect of the present application, a preparation method of lecithin emulsifier capable of maintaining emulsifying ability in an acidic environment is provided.
[0007] A preparation method of lecithin emulsifier capable of maintaining emulsifying ability in an acidic environment is carried out in the following steps:
[0008] (1) Preparation of Anemarrhena asphodeloides extract: After the dried root and stem of Anemarrhena asphodeloides is crushed, impurities are removed by ethyl acetate extraction, and then the precipitate is alcohol extracted to obtain the Anemarrhena asphodeloides extract;
[0009] (2) High-pressure homogenization treatment: lecithin is added to the Anemarrhena asphodeloides extract, and the mixture is fully stirred and mixed to obtain a mixed solution. The mixed solution is treated by high-pressure homogenization, and then dried to obtain the lecithin emulsifier.
[0010] The preparation of the Anemarrhena asphodeloides extract in step (1) above is specifically as follows: the crushed dried root and stem of Anemarrhena asphodeloides is dissolved in ethyl acetate at a ratio of (10-30) / 100 (g / mL) to remove impurities, and the extraction time is 30-60 min. When the precipitate is alcohol extracted, anhydrous ethanol is used as the solvent, and the volume of the anhydrous ethanol is the same as that of the ethyl acetate. The alcohol extraction temperature is 40-60℃, and the alcohol extraction time is 1-4 h.
[0011] In the high-pressure homogenization treatment in step (2) above, the addition ratio of lecithin in the Anemarrhena asphodeloides extract is 5-10 g / L.
[0012] In the high-pressure homogenization treatment in step (2) above, the stirring is preferably mechanical blade stirring, the stirring time is 0.5-1 h, the stirring temperature is 40-60℃, and the stirring speed is 30-60 r / min.
[0013] The high-pressure homogenization in the above step (2) is carried out at a homogenization pressure of 50-250 MPa, a homogenization temperature of 40-60 DEG C, and a homogenization time of 20-60 min.
[0014] In a second aspect of the present application, the lecithin emulsifier prepared by the above method is used for dehairing a cat.
[0015] The lecithin emulsifier prepared by the first aspect of the present application has an emulsifying ability in an acidic environment, can emulsify and remove the surface lipid layer of cat hair in the cat stomach, and further improves the contact rate of gastric acid, pepsin and other gastric proteins with the structural proteins under the lipid layer of cat hair, improves the damage of the cat stomach to the structure of cat hair, and promotes the softening of cat hair.
[0016] Further, the above lecithin emulsifier can also be applied to other pets such as rabbits, chinchillas and ferrets.
[0017] Further, the above lecithin emulsifier is fed to the cat by direct feeding or mixing into cat food (or snacks).
[0018] The present application has the following beneficial effects:
[0019] In the first step (preparation of the Anemarrhena asphodeloides extract) of the process for preparing the lecithin emulsifier according to the present application, the oil and impurity components such as icariin in Anemarrhena asphodeloides can be effectively removed by the ethyl acetate extraction step, and the purity of the effective components such as Anemarrhena asphodeloides saponin A3, B1 and B3 is improved.
[0020] Lecithin has good emulsifying properties in neutral and alkaline environments, and can emulsify oil, but its emulsifying effect is significantly reduced in an acidic environment. The Anemarrhena asphodeloides saponin contained in the Anemarrhena asphodeloides extract prepared according to the present application is a furostan-type steroidal saponin, which is stable in structure in an acidic environment, but has relatively weak emulsifying ability. The lecithin emulsifier prepared by high-pressure homogenization of the lecithin and the Anemarrhena asphodeloides alcohol extract has the following advantages compared with the use of lecithin alone:
[0021] 1. Beneficial effects when used in an acidic environment
[0022] (1) Maintaining emulsifying ability in an acidic environment
[0023] Lecithin belongs to the zwitterionic emulsifier (with both positive and negative charge groups), and has good emulsifying performance in neutral and weak alkaline environment. In acidic environment, the hydrophilic head of lecithin carries positive charge, and when the lecithin molecules are closely arranged on the oil / water interface, the electrostatic repulsion between the hydrophilic heads is large, which leads to the arrangement of lecithin not being close and stable, and further destroys the stability of the oil / water interface, resulting in the decrease of emulsifying capacity. The steroid saponin in the anemarrhena asphodeloides alcohol extract does not carry charge, and after mixing with lecithin and high-pressure homogenization processing, the steroid group of the steroid saponin is closely combined with the fatty acid group of lecithin by hydrophobic interaction, and the hydrophilic head of lecithin is combined with the hydroxyl group of the anemarrhena steroid saponin by hydrogen bond driving and further stabilizing the molecular structure, thereby forming a new type of lecithin emulsifier. Due to the introduction of the steroid saponin, the proportion of positive charge carried by the composite emulsifier in the acidic environment also decreases, which helps to reduce the repulsion strength between lecithin molecules and the interfacial tension, and further maintains the emulsifying capacity and stability of lecithin emulsifier in acidic environment.
[0024] (2) Improve the dispersing ability of lecithin
[0025] When lecithin carries a large amount of charge in an acidic environment, a large amount of opposite charges will be attracted and accumulated on its surface, generating a strong electric field effect, which counteracts the electrostatic repulsion and inhibits the formation of the internal hydration layer. The material exhibits aggregation and is difficult to disperse. If lecithin only carries a small amount of charge, the charge will not accumulate, and it will also help water molecules to form a hydration layer between material molecules, preventing the molecules from approaching and aggregating, and thus improving the dispersing ability of the material. The anemarrhena steroid saponin includes smilax saponin, anemarrhena saponin A3, isorhizoma saponin, anemarrhena saponin B1, anemarrhena saponin B3, etc. The saponin itself does not carry charge, and its hydrophilic group (polysaccharide glycoside) carries a large number of hydroxyl groups, which itself has a strong hydration ability. After being combined with lecithin, the amount of positive charge carried is greatly reduced, which promotes the formation of a hydration layer between lecithin molecules and prevents lecithin molecules from approaching and aggregating, and thus improves the dispersing ability of lecithin in an acidic environment. That is, emulsification can be completed without the need for vigorous mechanical stirring, which reduces the difficulty of emulsification and helps to complete the emulsification in the stomach.
[0026] 2. Beneficial effects in cat hair application
[0027] Lecithin requires high mechanical stirring to achieve good emulsification effect. The stomach peristalsis intensity of a cat is not sufficient to promote the dispersion of lecithin. According to the method of the present application, the lecithin emulsifier prepared by high-pressure homogenization of lecithin and anemarrhena steroid saponin has good emulsifying capacity and dispersibility in the acidic environment of the cat stomach, and can effectively remove the lipid layer on the surface of cat hair remaining in the cat stomach. This helps to increase the contact rate of gastric acid, pepsin and other substances in the cat stomach with the structural proteins under the lipid layer of cat hair, and thus effectively reduces the structural strength of the structural proteins, promotes the softening and discharge of cat hair in the cat stomach.
[0028] The lecithin emulsifier prepared in the present application has good emulsification performance and dispersion performance in an acid environment, and the emulsification chromatography index is reduced by 63.64% to 78.95% compared with the control, and the dispersion is increased by 2.64 to 5.87 times compared with the control. The lecithin emulsifier prepared in the present application has good emulsification and degreasing effect on cat hair in the cat stomach acid environment. In the simulated cat stomach digestion test, the degreasing rate of cat hair after adding the lecithin emulsifier is increased by 1.12 to 3.71 times compared with the control. In the cat feeding test, after supplementing the cat with the lecithin emulsifier, the cat hair can be effectively softened and discharged, and the discharge amount score is increased by 40.00% to 108.33% compared with the control; the peak force of the cat needle hair is reduced by 26.32% to 62.07% compared with the control; and it can be seen from the scanning electron microscope that the cat needle hair is obviously damaged after supplementing the cat with the lecithin emulsifier, the boundary between the scales is blurred, and only a small amount of scales remain, most of which have fallen off. BRIEF DESCRIPTION OF DRAWINGS
[0029] Figure 1 The scanning electron microscope pictures of the cat needle hair discharged in the feces are shown in the following table, and the magnification is 2000 times. A is the cat needle hair of the control example 1, B is the cat needle hair of the control example 2, C is the cat needle hair of the control example 3, D is the cat needle hair of the example 1, E is the cat needle hair of the example 2, and F is the cat needle hair of the example 3. DETAILED DESCRIPTION
[0030] In the present application, the terms used are generally understood by those of ordinary skill in the art, unless otherwise stated. The present application is further described in detail below in conjunction with specific examples and with reference to the data. It should be understood that these examples are only to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application, and those skilled in the art can make some non-essential improvements and adjustments to the present application according to the content of the above-mentioned application.
[0031] In the following examples, various processes and methods that are not described in detail are conventional methods known in the art. The source of the reagents used, the trade name, and if necessary, the composition of the reagents are indicated at the first occurrence, and thereafter the same reagents are used as indicated at the first occurrence unless otherwise specified.
[0032] In the present examples and control examples, the rhizoma notopterygii dried products and lecithin are commercially available.
[0033] In the present examples and control examples, the sample emulsification capacity (emulsion chromatography index) in an acid environment is determined by the following method:
[0034] The test sample was dissolved in 50% (v / v) ethanol solution at 1% (m / v), mixed with soybean oil at a volume ratio of 1:1, treated with a high-speed homogenizer at high speed (15000 r / min, 3 min), and then allowed to stand for 48 h. The height of the clear liquid layer Hc and the total height of the emulsion Ht were measured, and the emulsion layering index (%) = Hc x 100 / Ht. The lower the emulsion layering index, the weaker the layering, and the better the emulsifying ability of the test sample.
[0035] Sample dispersibility determination method in the present example and the control example:
[0036] The test sample was added to the simulated cat gastric juice at 1% (m / v) and immediately placed in a shaking bed (100 r / min, 39°C). The simulated cat gastric digestion was shaken for 30 min. After shaking, the filtrate was obtained by filtering with filter paper, and the absorbance (OD value) was measured at 600 nm by an enzyme marker. The higher the absorbance, the higher the turbidity of the filtrate, and the better the dispersibility of the test sample. 600
[0037] In-vitro simulated cat gastric digestion test method in the present example and the control example:
[0038] Simulated cat gastric juice: 0.4 g of pepsin was dissolved in 100 mL of hydrochloric acid solution (0.01 mol / L), and the pH was adjusted to 2.0.
[0039] The hair in a 3 cm x 3 cm area on the back of a cat was removed with an electric shaver, and the guard hairs with a length of more than 5 cm were selected and mixed. 0.1 g of the test sample was dissolved in 50 mL of simulated cat gastric juice, 0.2 g of cat guard hairs was added and stirred uniformly, and then allowed to stand at 39°C for 5 min. It was transferred to a constant-temperature shaking bed (39°C, 80 r / min) to simulate gastric digestion for 4 h. The cat guard hairs were washed with distilled water, the water was absorbed with filter paper, and the cat guard hairs were dried at 50°C for 3 h and stored under cool and dry conditions for testing.
[0040] Cat hair degreasing rate determination method in the present example and the control example:
[0041] The cat hair was subjected to Soxhlet extraction to determine the lipid content. The cat hair lipid degreasing rate was calculated according to formula (1):
[0042]
[0043] In the formula, P is the cat hair lipid degreasing rate, unit: %; f is the sample cat hair lipid content, unit: g / 100g; F is the lipid content of the cat hair treated only with drying, unit: g / 100g.
[0044] Cat feeding test method in the present example and the control example:
[0045] Recruit pet cats with poor hair shedding (with vomiting hair shedding), including British, Siamese, blue cat, American short hair, golden giraffe and Maine, 6 cats of each breed, randomly divided into 6 groups, each group contains 1 cat of the above 6 kinds. The test sample powder is directly mixed into the cat's daily diet at 0.3% (mass ratio) for supplementary feeding. After 3 days of supplementary feeding by mixing the diet, take the feces and wash out the cat hair from it, and conduct hair shedding score, take the cat hair and conduct peak force test, and take scanning electron microscope photos of the cat hair.
[0046] Hair shedding score method in this example and control example:
[0047] Take the cat feces and dry it at 60°C for 12h, crush it and pick the hair from it, wash it with distilled water for 3 times, dry it at 60°C for 2h, and store it at low temperature for observation and detection. Use 3-point scoring system, 0 points: feces does not contain hair; 1 point: feces contains a small amount of hair, not in a knot; 2 points: feces contains more hair and a small amount of hair knot; 3 points: feces contains more hair and more hair knot.
[0048] Hair shedding score method in this example and control example:
[0049] Take the cat's pin hair after drying, fix it vertically on the fixed clamp, and use the tensile tester to measure the instantaneous tensile value when a single hair is pulled off.
[0050] Cat feces pin hair scanning electron microscope shooting method in this example and control example:
[0051] Place the washed and dried cat pin hair fiber sample in the copper platform for gold spraying operation, and use the scanning electron microscope to take photos and observe.
[0052] Control Example 1
[0053] In the in vitro simulation of cat stomach digestion test, without adding any test sample, the hair degreasing rate was 9.46%. In the cat feeding test, without adding any test sample for supplementary feeding, the cat's hair shedding score was 1.2, the peak force of the cat's pin hair pulled off was 0.29N, and in the scanning electron microscope picture, the scales on the hair surface were clear, the boundaries were distinguishable, and a small amount of scales fell off.
[0054] Control Example 2
[0055] The preparation of Anemarrhena asphodeloides extract is carried out as follows:
[0056] The dried rhizome of Anemarrhena asphodeloides was pulverized, and 50g of the pulverized material was added to 500mL of ethyl acetate for extraction for 30min. The precipitate was then collected by filtration. The precipitate was added to 500mL of anhydrous ethanol and extracted at 40℃ for 4h. The filtrate was collected as the Anemarrhena asphodeloides extract. The ethanol extract was homogenized under high pressure at 250MPa, 40℃, and 60min. The liquid was then concentrated under reduced pressure, freeze-dried, and ground into powder to obtain the Anemarrhena asphodeloides extract. The emulsion chromatography index of the tested sample was 33%, and the OD of the filtrate in the dispersibility test was [not specified]. 600 The value is 0.023.
[0057] In an in vitro simulated feline gastric digestion experiment, the degreasing rate of cat hair was 15.25%. In a cat feeding experiment, the cat's hair excretion score was 1.4, the peak breaking force of the excreted cat hair was 0.23N, and in the scanning electron microscope images, the scales on the hair surface were relatively clear and the boundaries were identifiable, with a small amount of scale loss and minor damage on the scale surface.
[0058] Compare with Example 3
[0059] Lecithin processed products were prepared according to the following method:
[0060] 1 g of lecithin was dissolved in 100 mL of anhydrous ethanol and homogenized under high pressure at 250 MPa, 40 °C, and 60 min. The liquid was then concentrated under reduced pressure, freeze-dried, and ground into powder to obtain the lecithin processed product. The emulsion chromatography index of the tested sample was 38%, and the OD of the filtrate in the dispersibility test was [not specified]. 600 The value is 0.036.
[0061] In an in vitro simulated feline gastric digestion experiment, the degreasing rate of cat hair was 18.66%. In a cat feeding experiment, the cat's hair excretion score was 1.5, the peak breaking force of the excreted cat hair was 0.19N, and in the scanning electron microscope images, the scales on the hair surface were relatively clear and the boundaries were identifiable, with a small amount of scale loss and minor damage on the scale surface.
[0062] Example 1
[0063] The dried rhizome of *Anemarrhena asphodeloides* was pulverized, and 50g of the pulverized material was added to 500mL of ethyl acetate for extraction for 30min. The precipitate was then filtered and collected. The precipitate was added to 500mL of anhydrous ethanol and extracted at 40℃ for 4h. The filtrate was then collected as the *Anemarrhena asphodeloides* extract. 1g of lecithin was added to 100mL of the *Anemarrhena asphodeloides* extract and stirred at 30r / min at 60℃ for 0.5h to fully dissolve the lecithin. The solution was then homogenized under high pressure at 250MPa, 40℃, and 60min. The liquid was then concentrated under reduced pressure, freeze-dried, and ground into powder to obtain the lecithin emulsifier. The emulsion chromatography index of the tested sample was 11%, and the OD of the filtrate in the dispersibility test was [not specified]. 600 The value is 0.131.
[0064] In an in vitro simulated feline gastric digestion experiment, the degreasing rate of cat hair was 41.93%. In a cat feeding experiment, the cat's hair excretion score was 2.1, and the peak breaking force of the excreted cat hair was 0.13N. In the scanning electron microscope images, the hair was obviously damaged, the boundaries between the scales on the surface were blurred, only a few scales remained, and most of them were shed.
[0065] Example 2
[0066] The dried rhizome of *Anemarrhena asphodeloides* was pulverized, and 150g of the pulverized material was added to 500mL of ethyl acetate for extraction for 60min. The precipitate was then filtered and collected. The precipitate was added to 500mL of anhydrous ethanol and extracted at 60℃ for 1h. The filtrate was then collected as the *Anemarrhena asphodeloides* extract. 0.5g of lecithin was added to 100mL of the *Anemarrhena asphodeloides* extract and stirred at 60r / min at 40℃ for 1h to fully dissolve the lecithin. The solution was then homogenized under high pressure at 50MPa, 60℃, and 20min. The liquid was then concentrated under reduced pressure, freeze-dried, and ground into powder to obtain the lecithin emulsifier. The emulsion chromatography index of the tested sample was 8%, and the OD of the filtrate in the dispersibility test was [not specified]. 600 The value is 0.158.
[0067] In an in vitro simulated feline gastric digestion experiment, the degreasing rate of cat hair was 44.57%. In a cat feeding experiment, the cat's hair excretion score was 2.3, and the peak breaking force of the excreted cat hair was 0.14N. In the scanning electron microscope images, the hair was obviously damaged, the boundaries between the scales on the surface were blurred, only a few scales remained, and most of them were shed.
[0068] Example 3
[0069] After pulverizing 100g of the dried rhizome of *Anemarrhena asphodeloides*, extract with 500mL of ethyl acetate for 45min, filter, and collect the precipitate. Add the precipitate to 500mL of anhydrous ethanol and extract at 50℃ for 2h. Filter and collect the filtrate as *Anemarrhena asphodeloides* extract. Add 0.75g of lecithin to 100mL of *Anemarrhena asphodeloides* extract and stir at 45r / min at 50℃ for 0.75h to fully dissolve the lecithin. Homogenize the solution under high pressure at 150MPa, 50℃, and 40min. Concentrate the liquid under reduced pressure, freeze-dry, and grind to obtain lecithin emulsifier. The emulsion chromatography index of the tested sample was 12%, and the OD of the filtrate in the dispersibility test was... 600 The value is 0.145.
[0070] In an in vitro simulated feline gastric digestion experiment, the degreasing rate of cat hair was 39.58%. In a cat feeding experiment, the cat's hair excretion score was 2.5, and the peak breaking force of the excreted cat hair was 0.11N. In the scanning electron microscope images, the hair was obviously damaged, the boundaries between the scales on the surface were blurred, only a few scales remained, and most of them were shed.
[0071] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for preparing a lecithin emulsifier that maintains emulsifying ability in an acidic environment, characterized in that... Follow these steps: (1) Preparation of Anemarrhena asphodeloides extract: The dried rhizome of Anemarrhena asphodeloides was crushed and extracted with ethyl acetate to remove impurities, and then extracted with alcohol to obtain Anemarrhena asphodeloides extract; (2) High pressure homogenization: Lecithin was added to the extract of Anemarrhena asphodeloides, homogenized under high pressure, and then dried to obtain lecithin emulsifier.
2. The method for preparing a lecithin emulsifier that maintains emulsifying ability in an acidic environment according to claim 1, characterized in that... Preparation of Anemarrhena asphodeloides extract in step (1) above: Specifically, take the dried rhizome of Anemarrhena asphodeloides and crush it, dissolve it in ethyl acetate at a ratio of (10-30) / 100 (g / mL) to remove impurities, and extract for 30-60 min. When extracting the precipitate with alcohol, use anhydrous ethanol as solvent, add the same volume as ethyl acetate, extract at 40-60℃, and extract for 1-4 h.
3. The method for preparing a lecithin emulsifier that can maintain emulsifying ability in an acidic environment according to claim 1, characterized in that... In step (2) above, the high-pressure homogenization process involves adding lecithin to the Anemarrhena asphodeloides extract at a ratio of 5 to 10 g / L.
4. The method for preparing a lecithin emulsifier that can maintain emulsifying ability in an acidic environment according to claim 1, characterized in that... In step (2) above, the high-pressure homogenization process is preferably carried out by using mechanical blades for stirring. The stirring time is 0.5 to 1 hour, the stirring temperature is 40 to 60°C, and the stirring speed is 30 to 60 r / min.
5. The method for preparing a lecithin emulsifier that can maintain emulsifying ability in an acidic environment according to claim 1, characterized in that... The high-pressure homogenization process in step (2) above: the homogenization pressure is 50-250 MPa, the homogenization temperature is 40-60℃, and the homogenization time is 20-60 min.
6. The application of the lecithin emulsifier prepared according to claims 1-5 in cat hair softening has the effect of promoting the softening of cat hair.
7. The application of the lecithin emulsifier prepared according to claims 1-5 in the softening of fur in cats, and its use in softening the fur of other pets such as rabbits, chinchillas and ferrets.
8. The application of the lecithin emulsifier prepared according to claims 1-5 in cat hair removal, by feeding the cat directly or mixing it into cat food (or treats).