Application of isoorientin in preparation of cosmetics with oil control effect

Abstract

The invention discloses an application of isoorientin in preparation of cosmetics with an oil control effect. The concentration of the isoorientin in the cosmetics is 2-10 [mu] g / mL. Research finds that isoorientin has a remarkable effect on oil control, can be used for preparing cosmetics with the oil control effect, and should have a good market prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to a new application of isohumulones, in particular to the application of isohumulones in the preparation of cosmetics with oil control effect. BACKGROUND

[0002] Sebaceous glands are the components of the sebaceous follicle unit that secrete sebum. The embryonic development of sebaceous glands develops with the development of hair follicles and epidermal tissue from the 13th week to the 16th week of fetal development. New sebaceous glands usually do not develop after birth, but their size increases with age. Sebaceous glands are a multilobular holocrine accessory organ of the epidermis. Sebaceous gland cells are mainly responsible for the synthesis of neutral lipids. Terminally differentiated sebaceous gland cells lyse and secrete lipids through the follicle orifice to the skin surface. In addition to the production and release of sebum, sebaceous glands also have the functions of lubricating the skin and hair, providing thermoregulation and antibacterial activity. Although the full function of sebum in human skin remains to be determined, sebum is an indispensable component of the epidermal barrier and the skin immune system. Sebaceous gland cells express a variety of hormone receptors, and the synthesis of sebum is regulated by hormones, especially androgens. Sebaceous gland dysfunction mainly includes sebaceous gland hyperplasia, sebaceous gland tumor, sebaceous gland carcinoma, sebaceous gland nevus and follicular sebaceous gland cystic hamartoma. Sebaceous glands are also involved in acne vulgaris, seborrheic dermatitis and androgenetic alopecia. At present, the clinical treatment adopts the method of combining topical drugs such as isotretinoin and benzoyl peroxide with optical treatment to treat abnormal sebaceous secretion. Although isotretinoin is the most effective sebaceous secretion inhibitor, it also has the risk of teratogenicity. In recent years, many natural sources of lipid secretion inhibitors have become a research hotspot due to their milder and side effect-free advantages, and many polyphenols, flavonoids and plant extracts have shown certain effects in inhibiting lipid secretion. Isohumulones also belong to natural active substances, which exist in many plants. The known effects include antioxidant, anti-inflammatory activity, cardiovascular protection, neuroprotection, blood glucose regulation, antibacterial activity, etc. At present, it has not been known that isohumulones have effects in inhibiting oil secretion. SUMMARY

[0003] The purpose of the present application is to provide the application of isohumulones in the preparation of cosmetics with oil control effect. The present application finds that isohumulones have significant effects in oil control and can be used for the preparation of cosmetics with oil control effect, which should have good market prospects.

[0004] The technical scheme of the present application is the application of isohumulones in the preparation of cosmetics with oil control effect.

[0005] In the foregoing application, the concentration of isohumulones in the cosmetics is 2-10 μg / mL.

[0006] In the aforementioned application, the concentration of the isosalipurposide in the cosmetic is 2 μg / mL, 5 μg / mL or 10 μg / mL.

[0007] Compared with the prior art, the application has the beneficial effects that the application of the isosalipurposide in the preparation of the cosmetic with the oil control effect is provided. The isosalipurposide has remarkable effects in the oil control through the model experiment, the isosalipurposide is a mild natural active substance, has small toxic and side effects on the human body, and should have broad market application prospects. BRIEF DESCRIPTION OF DRAWINGS

[0008] Figure 1 is a column chart of the detection results of the cell viability of SZ95 cells under different concentrations of LA.

[0009] Figure 2 is a graph of the detection results of the cell viability of SZ95 cells under different concentrations of isosalipurposide.

[0010] Figure 3 is a comparison graph of the staining effects of each group in Table 3.

[0011] Figure 4 is a comparison graph of the fluorescence intensities of each group in Table 3. DETAILED DESCRIPTION

[0012] The application will be further described below in combination with the drawings and examples, but is not taken as the basis for limiting the application.

[0013] Example: I. Oil control active substance - isosalipurposide.

[0014] Step 1.1): After the recovery of the sebaceous gland cells (SZ95), the cell growth condition is observed, the cells are counted when the cell coverage rate reaches more than 80%, and the cells are inoculated into a plurality of 96-well plates and a plurality of 24-well plates. Among them, the 96-well plates are used for detecting the cell survival rate, and the 24-well plates are used for detecting the Nile red staining of the sebaceous gland cells. The well plates are placed in a CO2 incubator (37°C, 5% CO2) for incubation overnight.

[0015] Step 1.2): Liquid preparation: prepare a plurality of sample working solutions, including linoleic acid (LA) working solution and isosalipurposide working solution.

[0016] Step 1.3): When the cell plating rate reaches about 60%, grouping and dosing are performed. Each group has 3 replicate wells.

[0017] Step 1.3.1): Take the linoleic acid (LA) working solution, group according to Table 1 as follows, and perform the LA experimental concentration exploration. The LA concentration in the range of 5-1000 μM is selected for the cell survival rate experiment.

[0018] Table 1 Step 1.3.2): Take the isofraxidin working solution, and group according to Table 2 below to explore the SZ95 cell experiment concentration of isofraxidin. Select a concentration of 0.5-100 μg / mL for the cell survival rate experiment.

[0019] Table 2 Step 1.3.3): According to step 1.3.1), the optimal modeling concentration of LA is determined to be 100 μM. According to Table 3, select the isofraxidin sample group within the safe concentration range of 0.5-100 μg / mL for Nile red staining and photographing to detect the SZ95 cell lipid droplet secretion.

[0020] Table 3 Step 1.3.4): The specific steps of Nile red staining are as follows: Rinse: Discard the culture medium and wash with PBS 3 times; Fix: Add 4% paraformaldehyde (300 μL) for 30 min; Staining: Prepare Nile red staining solution (working concentration is 10 μg / mL), discard the fixing solution, rinse the cells with PBS 3 times, add Nile red staining solution (300 μL / well) for 15 min; Photograph: Under an inverted fluorescence microscope, observe the staining of each group of cells and take pictures.

[0021] Result analysis: Use Image Pro Plus software to quantitatively analyze the fluorescence intensity.

[0022] II. Data processing Step 2.1): Cell viability test, for each group in Table 1 and Table 2, the specific method is as follows: discard the supernatant in the 96-well plate, add 100 μL of CCK-8 working solution (CCK8 mother liquor: culture medium = 1:9) to each well, and incubate in an incubator (37°C, 5% CO2) for 2 hours. Then use a microplate reader to measure the absorbance at 450 nm.

[0023] Step 2.2): Cell survival rate, for each group in Table 1 and Table 2, the inhibition rate is calculated: Cell survival rate (%) = [(As-Ab) / (Ac-Ab)]x100% As: Experimental well absorbance (containing cells, culture medium, CCK-8 solution and drug solution); Ac: Control well absorbance (containing cells, culture medium, CCK-8 solution, without drug); Ab: Absorbance of blank wells (containing culture medium and CCK-8 solution, but excluding cells and drugs).

[0024] Step 2.3): Nile Red staining: Rinse, fix cells, stain with Nile Red, and then take pictures using a fluorescence microscope.

[0025] Step 2.4): Statistical analysis of results: Quantitative analysis of fluorescence intensity was performed using Image Pro Plus software, and graphs were plotted using GraphPad Prism. The results are presented as Mean+SEM. t-tests were used for comparisons between groups. P < 0.05 was considered statistically significant, and P < 0.01 was considered highly statistically significant.

[0026] III. Experimental Results Step 3.1): Regulation of isohypogonin lipid droplet secretion: For each group in Table 1, such as Figure 1 As shown in Table 4, SZ95 cells exhibited a viability of over 70% and low toxicity when LA concentrations ranged from 5 to 100 μM, indicating that further experiments could be conducted.

[0027] Table 4 For each group in Table 2, such as Figure 2 As shown in Table 5, when isochorin was used at concentrations ranging from 0.5 to 10 μg / mL, SZ95 cells exhibited a viability of over 90% and no cytotoxicity.

[0028] Table 5 Step 3.2.2): Based on the staining results of each group in Table 3, the data is processed to obtain... Figure 3 and Figure 4 As a result, from Figure 3 and Figure 4 It is evident that the LA group significantly increased lipid droplet secretion compared to the blank control group; finasteride and isosorbide Vate significantly reduced lipid droplet secretion compared to the blank control group; and isosorbide Vate at concentrations of 2 μg / mL, 5 μg / mL, and 10 μg / mL all significantly reduced lipid droplet secretion compared to the blank control group, demonstrating oil-controlling effects.

[0029] Therefore, isoharmonin has an oil-controlling effect and can be used in the preparation of cosmetics with oil-controlling effects. The concentration range is 2~10μg / mL, preferably 2μg / mL, 5μg / mL or 10μg / mL.

Claims

1. Use of isobacillin in the preparation of a cosmetic product with oil control effect.

2. Use according to claim 1, characterized in that: The concentration of the isobacillin in the cosmetic product is 2-10 μg / mL.

3. Use according to claim 2, characterized in that: The concentration of the isobacillin in the cosmetic product is 2 μg / mL, 5 μg / mL or 10 μg / mL.

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