Anti-shedding hair care composition and method of making and using same

Abstract

The application discloses a hair loss prevention and care composition and a preparation method and application thereof, and relates to the field of hair care products, and specifically discloses a hair loss prevention and care composition which comprises extracts of Radix Polygoni Multiflori, Rhizoma Polygonati, Cacumen Platycladi, Fructus Rosae Laevigatae, Fructus Zanthoxyli and Ampelopsis megalophylla, and has good compatibility, and has the effects of antioxidation, DHT inhibition, antibiosis and inflammation inhibition and moisture retention. According to the physical properties of plant active ingredients, the Ampelopsis megalophylla leaf extract and the Ylang Ylang leaf extract are prepared by combining various extraction methods, so that the extraction rate of active substances is improved, the leaching of harmful substances is reduced, and the efficacy and safety of the composition are improved.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to an anti-hair loss and hair care composition, its preparation method, and its application. Background Technology

[0002] Hair loss has become a health and aesthetic problem affecting hundreds of millions of people worldwide. It not only impacts appearance but also easily triggers anxiety, low self-esteem, and other psychological issues, significantly reducing quality of life. Based on pathological mechanisms, hair loss is mainly classified into: androgenetic alopecia (AGA), alopecia areata (AA), telogen effluvium (TE), and cicatricial alopecia. AGA accounts for over 90% of cases (clinical studies show that AGA accounts for 95% of male hair loss patients and 70%-90% of female hair loss patients), making it a core pathological type that urgently needs to be addressed in the field of hair loss research. In Traditional Chinese Medicine (TCM) theory, hair is considered "the surplus of blood and the essence of the kidneys." AGA in TCM is associated with liver and kidney deficiency, accompanied by blood heat and wind-dryness, and damp-heat accumulation.

[0003] Currently, hair and scalp care products containing Chinese herbal extracts on the market are slow to show results. This is partly due to poor compatibility, as they do not take into account moisturizing, wind-dispelling, and antibacterial properties; and partly due to limitations in extraction methods, resulting in a lower content of active ingredients.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] This invention provides an anti-hair loss and hair care composition, its preparation method, and its application. By compounding Polygonum multiflorum extract, Polygonatum sibiricum extract, Platycladus orientalis leaf extract, Liquidambar formosana extract, Zanthoxylum bungeanum extract, and Ampelopsis japonica leaf extract, and selecting or combining multiple extraction methods according to the physical properties of the plant active ingredients, a hair loss and hair care ingredient with antioxidant, DHT inhibition, anti-inflammatory, antibacterial, and moisturizing effects is prepared.

[0006] This invention is implemented as follows:

[0007] In a first aspect, the present invention provides an anti-hair loss and hair care composition comprising Polygonum multiflorum extract, Polygonatum sibiricum extract, Platycladus orientalis leaf extract, Liquidambar formosana extract, Zanthoxylum bungeanum extract and Ampelopsis grossedentata leaf extract.

[0008] In Traditional Chinese Medicine (TCM) theory, Polygonum multiflorum (He Shou Wu) is believed to nourish the liver and kidneys, replenish essence and blood, and darken hair; Polygonatum sibiricum (Huang Jing) is believed to replenish qi and nourish yin, strengthen the spleen, moisten the lungs, and benefit the kidneys; Platycladus orientalis (Ce Bai Ye) is believed to cool the blood, stop bleeding, clear heat, and moisten dryness; Ampelopsis pilosula (Xian Fang Gu Lao Ye) is not a common medicinal herb, but it is sweet, bland, and cool in nature, and enters the lung, liver, and stomach meridians, having the effects of clearing heat and detoxifying, reducing swelling, and promoting diuresis; the combination of Platycladus orientalis (Ce Bai Ye) and Ampelopsis pilosula (Xian Fang Gu Lao Ye) has a synergistic effect of clearing heat, cooling the blood, and resolving dampness; and Liquidambar formosana (Lu Lu Tong) is believed to dispel wind and activate blood circulation, thus clearing blockages. The meridians and blood flow of the scalp are cleared, and stagnation is removed, thus guiding the essential substances of tonifying herbs such as Polygonum multiflorum and Polygonatum sibiricum to better reach the hair follicles and exert their nourishing effect. Sichuan pepper has the effects of warming the middle and dispelling cold, removing dampness, relieving pain, killing insects and relieving itching. Its pungent and warm properties can warm and unblock the meridians and promote blood circulation. When applied externally to the scalp, it can stimulate the local skin, dilate capillaries, and improve microcirculation, thereby providing more nutrients to the hair follicles. At the same time, its insecticidal and antipruritic effects can also assist Ampelopsis japonica leaves in cleaning the scalp environment.

[0009] Traditional Chinese medicine (TCM) prescriptions emphasize the principle of "principal, assistant, adjuvant, and guide" in drug formulation, aiming to enhance efficacy and reduce side effects through the synergistic combination of different herbs. In this herbal composition, Polygonum multiflorum (He Shou Wu) and Polygonatum sibiricum (Huang Jing) serve as the principal herbs. Polygonum multiflorum tonifies the liver and kidneys, replenishes essence and blood, directly addressing the root cause of hair loss and graying due to "kidney deficiency and blood deficiency." Polygonatum sibiricum tonifies qi and nourishes yin, replenishes kidney essence, and complements Polygonum multiflorum, working together as the foundation for nourishment. Their function is to "treat the root cause," providing the internal material basis for hair regeneration and health, reflecting the TCM therapeutic philosophy of "strengthening the body's resistance and consolidating its foundation." As an adjuvant herb, the primary function of arborvitae leaves is to assist the principal herb in strengthening the therapeutic effect. It has a clear effect on promoting hair growth and darkening hair, and its slightly cold nature allows it to cool the blood and clear heat, specifically targeting hair loss caused by "blood heat." This not only balances the warming and tonifying properties of the principal herb, avoiding the potential problems of excessive tonification that could harm the stomach or exacerbate damp heat, but also expands the applicability of the formula, making it equally effective for hair loss types characterized by "mixed deficiency and excess" (both underlying liver and kidney deficiency and superficial blood heat). As an adjuvant herb, ampelopsis leaves serve three purposes: first, to assist the principal and adjuvant herbs in strengthening their therapeutic effects; second, to treat secondary symptoms; and third, to restrain the toxic side effects or overly potent properties of the principal and adjuvant herbs. The leaves of *Ampelopsis grossedentata* are sweet, bland, and cool in nature, possessing the effects of clearing heat and detoxifying, calming the liver and promoting diuresis. They primarily address the "symptoms," namely localized damp-heat, inflammation, and fungal infections of the scalp (such as dandruff, oily scalp, and itching). In synergy with *Platycladus orientalis* leaves, they enhance the cleansing and conditioning effects on the scalp environment commonly associated with seborrheic alopecia, creating a favorable "soil" environment for the principal herbs, allowing the nourishing effects to be effectively exerted. *Luffa cylindrica* and Sichuan pepper are used as adjuvant herbs. *Luffa cylindrica*, with its characteristic of "unblocking the twelve meridians," can dredge the scalp's pores and meridians, guiding the power of other herbs upwards to the head and face, directly into the hair follicles, ensuring that nutrients are effectively absorbed by the hair follicles. Sichuan pepper, with its pungent and warm properties, can warm and unblock the blood vessels, promote blood circulation, remove blood stasis, and stimulate local circulation. It acts like a "booster," enhancing blood supply to the scalp and allowing the essence and blood from Polygonum multiflorum and Polygonatum sibiricum to better nourish the hair roots. These two herbs play more of the roles of "guiding herbs" and "blood-activating herbs," directing the medicinal power to the affected area (scalp and hair follicles) and harmonizing the effects of other herbs.

[0010] This composition has the effects of treating both the root cause and the symptoms, combining tonification and purgation, regulating both internal and external factors, and using both cold and hot properties. Specifically, it uses Polygonum multiflorum and Polygonatum sibiricum to nourish the liver and kidneys as the "root cause treatment," while Platycladus orientalis leaves, Ampelopsis japonica leaves, and Zanthoxylum bungeanum leaves to cleanse the scalp environment and improve local circulation as the "symptom treatment." It combines the "tonification" of Polygonum multiflorum and Polygonatum sibiricum with the "purgation" (clearing heat, cooling blood, and drying dampness) of Ampelopsis japonica leaves and Platycladus orientalis leaves, avoiding the bias of one-sided medication. The principal drug focuses on the internal generation of essence and blood, while the adjuvant drugs focus on improving the external scalp environment and unblocking qi and blood. The formula contains the cold properties of Ampelopsis japonica leaves and Platycladus orientalis leaves, the pungent and warm properties of Zanthoxylum bungeanum, the slightly warm properties of Polygonum multiflorum, and the neutral properties of Polygonatum sibiricum, making the overall medicinal properties more balanced, reducing the irritation to users with different constitutions, and broadening the applicable population.

[0011] In modern pharmacology, Polygonum multiflorum contains active ingredients such as stilbene glycosides, anthraquinones (such as emodin), and flavonoids, which can nourish hair follicles, prolong the hair growth phase, promote scalp blood circulation, and have antioxidant and anti-inflammatory effects. Polygonatum sibiricum contains active ingredients such as polysaccharides, saponins, and flavonoids, which can effectively resist oxidation and moisturize, helping to slow down scalp and hair follicle aging and provide nourishment for hair. Platycladus orientalis leaves contain active ingredients such as flavonoids, volatile oils (terpenes), and tannins, which can inhibit the activity of 5α-reductase, thereby reducing dihydrotestosterone (DHT). The product contains T, as well as anti-inflammatory, antioxidant, and hair follicle metabolism-promoting functions; Sichuan pepper contains volatile oils, polyphenols, alkaloids, sanshool and other active substances, which have antioxidant and antibacterial properties, help cleanse the scalp, and reduce oxidative stress damage to hair follicles; Ampelopsis japonica leaves contain flavonoids, gallotannins and other active ingredients, which can effectively resist oxidation and inflammation, help protect scalp cells from free radical damage, and soothe the scalp; Luffa cylindrica contains terpenes, steroids, organic acids, volatile oils and other active ingredients, which play an anti-inflammatory, antibacterial and antioxidant role.

[0012] In some embodiments, the anti-hair loss and hair care composition also includes ylang-ylang (Cananga odorata) leaf extract.

[0013] Ylang-ylang leaf extract is rich in antibacterial and anti-inflammatory active substances. The terpenes and terpenoids in it, including monoterpenes (such as α-pinene and linalool) and sesquiterpenes (such as β-caryophyllene, geranium, α-farnesene, and juniperene), are not only important sources of aroma, but have also been shown to have anti-inflammatory, antibacterial, and insecticidal biological activities. It also contains phenolic compounds, including eugenol and p-tolyl methyl ether, which usually have significant antioxidant and antibacterial activities.

[0014] In some embodiments, the anti-hair loss and hair care composition comprises the following ingredients in parts by weight: 4-6 parts of Polygonum multiflorum extract, 3-6 parts of Polygonatum sibiricum extract, 4-6 parts of Platycladus orientalis leaf extract, 1-2 parts of Liquidambar formosana extract, 1-2 parts of Zanthoxylum bungeanum extract, 2-3 parts of Ampelopsis japonica leaf extract, and 0-4 parts of Ylang Ylang leaf extract.

[0015] In some embodiments, the preparation method of the Amaranthus fasciatus leaf extract includes the following steps: (1) washing fresh Amaranthus fasciatus leaves, drying them first, and then pulverizing them to obtain Amaranthus fasciatus leaf powder; (2) mixing the Amaranthus fasciatus leaf powder and the extract, performing ultrasonic treatment and / or microwave treatment, separating the solid and liquid phases, and drying the liquid phase a second time to obtain the Amaranthus fasciatus leaf extract.

[0016] In some embodiments, the first drying is selected from blower drying or freeze drying.

[0017] In some embodiments, the second drying includes the following steps: placing the liquid phase in an ice bath at -5~0℃ and stirring at 30~100 rpm for 30 min, then cooling it to -20℃ at -1~-5℃ / min, maintaining the stirring speed unchanged for 60 min, centrifuging to obtain a second liquid phase, pre-cooling it to -60~-40℃, and then drying it at -20~-10℃, with a vacuum degree of 20~50 Pa and a drying time of 24~48 h.

[0018] In some embodiments, the extract comprises, by mass percentage, the following components: 0.01%–0.1% sodium carbonate, 0.05%–0.1% disodium ethylenediaminetetraacetate, 0.1%–0.3% antioxidant, with the balance being water; the antioxidant is selected from at least one of sodium sulfite, ascorbic acid, and tert-butyl-p-hydroxyanisole; the disodium ethylenediaminetetraacetate in the extract can chelate calcium contained in plant cell walls. 2+ Mg 2+ It removes metal ions, weakens cell wall structure, and increases its permeability. At the same time, it can chelate metal ions in the solution and inhibit the metal ion-catalyzed polyphenol oxidation reaction. Antioxidants are also added to the extract. They are more easily oxidized than polyphenols, thus protecting polyphenols from oxidation. They can also reduce oxidized quinones and reverse the browning process. Antioxidants and disodium EDTA synergistically inhibit polyphenols from oxidative browning and increase the content of active ingredients in the extract.

[0019] In some embodiments, the pH of the extract is 8-10; the extract is weakly alkaline, which is conducive to the dissociation of phenolic substances into phenolic anions, increasing their polarity and thus significantly improving their solubility in water; at the same time, the alkalinity can destroy hemicellulose and pectin in plant cell walls, making the cell structure looser and facilitating the dissolution of the contents.

[0020] In some embodiments, the mixing ratio of the *Ampelopsis grossedentata* leaf powder and extract is 1 g: 3-10 mL.

[0021] In some embodiments, the ultrasonic treatment has a power of 200-600W, a frequency of 20-40kHz, a duration of 30-60min, and a temperature of 20-40℃.

[0022] In some embodiments, the microwave processing power is 500~1000W, the time is 30min, and the temperature is 20~50℃.

[0023] In some embodiments, the method for preparing the ylang-ylang leaf extract includes the following steps:

[0024] (1) Wash, dry and crush fresh ylang-ylang leaves to obtain ylang-ylang leaf powder;

[0025] (2) The ylang-ylang leaf powder was subjected to supercritical CO2 extraction to obtain the first extract and ylang-ylang leaf residue;

[0026] (3) The ylang-ylang leaf residue is mixed with a low eutectic solvent and water, and subjected to ultrasonic treatment and / or microwave treatment. After centrifugation, acid or alkali is added to the resulting liquid phase to adjust the pH of the second extract to 6.8-7.2 to obtain the second extract.

[0027] (4) Combine the first extract and the second extract, and then freeze-dry them to obtain ylang-ylang leaf extract.

[0028] Ylang-ylang leaves contain alkaloids such as liriodenine, which possess antitumor and topoisomerase II inhibitory activities, but also exhibit cytotoxicity to healthy cells. Therefore, optimized extraction methods are required to retain non-polar active components such as terpenes and terpenoids, as well as polar active components such as phenols, while reducing the dissolution of alkaloids (which typically exist in plants as alkaloid salts, exhibiting good water and alcohol solubility). Existing technologies use solvent extraction with ethanol, petroleum ether, or methanol, and the resulting extracts have been shown to have strong anti-inflammatory, antioxidant, and antibacterial activities. However, solvent extraction with ethanol, petroleum ether, or methanol leads to the loss of volatile terpenoids during post-processing and lacks selectivity for the extract, thus also resulting in the dissolution of alkaloids. This invention first uses supercritical CO2 fluid to extract non-polar substances, and then uses a specific low-eutectic solvent to extract polar active substances. The combined extract has a higher content of active substances and a lower content of alkaloids, demonstrating higher efficacy and safety.

[0029] In some embodiments, the eutrophic solvent comprises histidine, glycerophosphate, and glycerol in a molar ratio of 7:2:9. This specific eutrophic solvent is a ternary eutrophic solvent, which includes histidine, a strong hydrogen bond acceptor with strong positive charge (strong basicity), glycerophosphate, a strong hydrogen bond donor, and glycerol, a weak hydrogen bond donor. The three form a stable non-covalent interaction through a hydrogen bond network. The strong hydrogen bond acceptor competes with the alkaloid salt for the anion, thereby converting the alkaloid salt into free alkaloids. Moreover, since the weak hydrogen bond donor contained in the hydrogen bond donor is difficult to re-salt with the free alkaloids, the alkaloids are retained in the plant, reducing their water solubility and decreasing the cytotoxicity of the extract.

[0030] In some embodiments, the supercritical CO2 extraction pressure is 35~45MPa, the extraction temperature is 35~50℃, the extraction time is 30~120min, and the supercritical CO2 flow rate is 20~30kg / h.

[0031] In some embodiments, the mass ratio of the ylang-ylang leaf residue to the eutectic solvent and water is 3~4:10~20:10.

[0032] In some embodiments, the method for preparing the low eutectic solvent is as follows: glycerol is heated to 80-90°C, then histidine and glycerophosphate are added, and the mixture is kept warm and stirred until it forms a transparent and homogeneous liquid, which is then cooled for later use.

[0033] In some embodiments, the acid is citric acid or acetic acid; the base is sodium hydroxide.

[0034] In some embodiments, the freeze-drying process is as follows: pre-cooling to -60 ~ -40°C, and then drying at -20 ~ -10°C, with a vacuum degree of 20 ~ 50 Pa and a drying time of 24 ~ 48 h.

[0035] Secondly, the present invention provides the use of any of the above-mentioned anti-hair loss and hair care compositions in the preparation of hair care products having the effects of promoting hair growth and / or strengthening hair and preventing hair loss.

[0036] Thirdly, the present invention provides an anti-hair loss and hair care essence, comprising 10wt% to 50wt% of any of the above-described anti-hair loss and hair care compositions.

[0037] In some embodiments, the hair loss prevention and hair care essence comprises, by weight percentage, the following components: 10wt%~30wt% hair loss prevention and hair care composition, 1wt%~5wt% surfactant, 0.05wt%~0.1wt% preservative, 10wt%~30wt% moisturizer, 0.02wt%~0.1wt% pH adjuster, and the balance being water.

[0038] In some embodiments, the surfactant is selected from at least one of sodium dodecylbenzenesulfonate, polysorbate-20, PEG-40 hydrogenated castor oil, and octyl / decyl glucoside.

[0039] In some embodiments, the preservative is selected from at least one of phenoxyethanol, ethylhexylglycerin, and p-hydroxyacetophenone.

[0040] In some embodiments, the humectant is selected from at least one of 1,3-propanediol, butylene glycol, glycerin, panthenol, amino acids, and β-glucan.

[0041] In some embodiments, the pH adjuster is selected from at least one of citric acid, lactic acid, sodium hydroxide, and triethanolamine.

[0042] Fourthly, the present invention provides a method for preparing an anti-hair loss hair care essence, comprising the following steps: mixing surfactant, humectant, preservative and water at 60-65°C to obtain a premix; cooling the premix to 40-45°C, adding the anti-hair loss hair care composition and stirring evenly; finally adding a pH adjuster to adjust the pH to 6.8-7.2 and defoaming to obtain the anti-hair loss hair care essence.

[0043] The present invention has the following beneficial effects:

[0044] (1) The present invention provides a hair loss prevention and hair care composition, which is prepared by the good compatibility of various Chinese medicines to obtain hair loss prevention and hair care ingredients with antioxidant, DHT inhibition, antibacterial and anti-inflammatory and moisturizing effects.

[0045] (2) Based on the physical properties of plant active ingredients and combined with various extraction methods, the present invention extracts Alopecurus dentata leaf extract and Ylang Ylang leaf extract, which improves the extraction rate of active substances while reducing the leaching of harmful substances, and at the same time improves the efficacy and safety of the composition. Attached Figure Description

[0046] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0047] Figure 1 To test the growth of hair on the backs of mice on days 3, 5, and 12 after drug administration in Example 7;

[0048] Figure 2 The images show pathological sections of mouse skin on days 10 and 20 after drug administration in Test Example 7. Detailed Implementation

[0049] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0050] In the description of the embodiments of this application, the technical terms "first" and "second" are used only to distinguish different objects and should not be construed as indicating or implying relative importance or implicitly indicating the number, specific order, or primary and secondary relationship of the indicated technical features.

[0051] The term "embodiment" in this document means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that is mutually exclusive with other embodiments.

[0052] In the embodiments of this application, the term "or / and" is only a description of the relationship between related objects, indicating that there can be three kinds of relationships. For example, A or / and B can represent three situations: A exists alone, A and B exist at the same time, and B exists alone.

[0053] Additionally, the character " / " in this article generally indicates that the objects before and after it are in an "or" relationship.

[0054] In the embodiments of this application, "multiple" means two or more (including two), similarly, "multiple groups" means two or more (including two groups), and "multiple layers" means two or more (including two layers), unless otherwise explicitly specified and limited.

[0055] In the embodiments of this application, "at least one" means one or more.

[0056] Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this application according to the specific circumstances.

[0057] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0058] Example 1

[0059] This embodiment provides a hair loss prevention and hair care composition, comprising the following raw materials in parts by weight: 5 parts Polygonum multiflorum extract, 3 parts Polygonatum sibiricum extract, 5 parts Platycladus orientalis leaf extract, 2 parts Liquidambar formosana extract, 1 part Zanthoxylum bungeanum extract, 2 parts Ampelopsis japonica leaf extract and 3 parts Ylang-ylang leaf extract.

[0060] The preparation method of the Ampelopsis grossedentata leaf extract is as follows:

[0061] (1) Wash the fresh Amaryllis leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain Amaryllis leaf powder; (2) Mix the Amaryllis leaf powder and extract at a ratio of 1g:5mL, microwave them, centrifuge them to obtain liquid phase 1, freeze-concentrate them, centrifuge them again to obtain liquid phase 2, and freeze-dry them to obtain Amaryllis leaf extract.

[0062] The extract, by mass percentage, comprises the following components: 0.1% sodium carbonate, 0.05% disodium ethylenediaminetetraacetate, 0.2% ascorbic acid, with the remainder being water; the pH of the extract is 9.8; the microwave treatment conditions are as follows: power 1000W, time 30min; the specific operation for freeze concentration is as follows: liquid phase 1 is placed in an ice bath at -5℃ and stirred at 60rpm for 30min, then cooled to -20℃ at -2℃ / min, maintaining the stirring speed for 60min, and the ice-water mixture is centrifuged to obtain liquid phase 2; the specific operation for freeze drying is as follows: pre-cooled to -60℃, then dried at -20℃ under a vacuum of 20Pa for 24h.

[0063] The preparation method of the ylang-ylang (Cananga odorata) leaf extract is as follows:

[0064] (1) Wash fresh ylang-ylang leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain ylang-ylang leaf powder; (2) Extract the ylang-ylang leaf powder with supercritical CO2 to obtain the first extract and ylang-ylang leaf residue; (3) Mix the ylang-ylang leaf residue with a low eutectic solvent and water, microwave it, centrifuge it, and add citric acid to the obtained liquid phase to adjust the pH of the second extract to 6.8 to obtain the second extract; (4) Combine the first extract and the second extract, and then freeze-dry them to obtain the ylang-ylang leaf extract.

[0065] The supercritical CO2 extraction conditions were as follows: extraction pressure of 42 MPa, extraction temperature of 50℃, extraction time of 120 min, and supercritical CO2 flow rate of 25 kg / h. The eutectic solvent consisted of histidine, glycerophosphate, and glycerol in a molar ratio of 7:2:9. The eutectic solvent was prepared by heating glycerol to 85℃, then adding histidine and glycerophosphate, stirring and maintaining the temperature until the mixture formed a transparent and homogeneous liquid, and then cooling it for later use. The mass ratio of ylang-ylang leaf residue to the eutectic solvent and water was 4 g:20 g:10 g. The microwave treatment conditions were as follows: power of 600 W, temperature of 50℃, and time of 20 min. The freeze-drying operation was as follows: pre-cooling to -60℃, then drying at -20℃ under a vacuum of 20 Pa for 24 h.

[0066] The extracts specifically include: Polygonum multiflorum root extract (processed), purchased from Lanzhou Waterles Biotechnology Co., Ltd., in solid powder form, extracted with ethanol / water; Polygonatum sibiricum extract (rhizome extract), purchased from Shanyang Lianfeng Biotechnology Co., Ltd., in solid powder form, extracted with water; Platycladus orientalis leaf extract, purchased from Lanzhou Waterles Biotechnology Co., Ltd., in solid powder form, extracted with water; Liquidambar formosana fruit extract, purchased from Lanzhou Waterles Biotechnology Co., Ltd., in solid powder form, extracted with water; and Zanthoxylum bungeanum extract (fruit extract), purchased from Shanyang Lianfeng Biotechnology Co., Ltd., in solid powder form, extracted with water.

[0067] Example 2

[0068] The difference from Example 1 is that the Ampelopsis grossedentata leaf extract in the anti-hair loss and hair care composition is extracted by water extraction, that is, in the preparation method of Ampelopsis grossedentata leaf extract, the extract is pure water.

[0069] Example 3

[0070] The difference from Example 1 is that ylang-ylang leaf extract is not added to the anti-hair loss and hair care composition.

[0071] Example 4

[0072] The difference from Example 1 is that the ylang-ylang leaf extract in the anti-hair loss and hair care composition is extracted with 70% alcohol and water. The specific preparation method of the ylang-ylang leaf extract is as follows: (1) Wash the fresh ylang-ylang leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain ylang-ylang leaf powder; (2) Mix the ylang-ylang leaf powder with an alcohol and water solution of 70% by mass of ethanol at a ratio of 1g:5mL and microwave treatment. After centrifugation, the liquid phase and residue are obtained. The residue is extracted twice as described above. All liquid phases are combined and vacuum dried at 50°C for 24 hours. Then, the liquid phase is frozen concentrated and freeze-dried to obtain the ylang-ylang leaf extract.

[0073] The microwave processing conditions were as follows: power 600W, temperature 50℃, time 20min; freeze concentration and freeze drying were the same as in Example 1.

[0074] Example 5

[0075] The difference from Example 1 is that the preparation method of the ylang-ylang leaf extract in the anti-hair loss and hair care composition does not include a low co-solubilizing solvent.

[0076] Example 6

[0077] This embodiment provides a hair loss prevention and hair care composition, comprising the following raw materials in parts by weight: 4 parts Polygonum multiflorum extract, 6 parts Polygonatum sibiricum extract, 4 parts Platycladus orientalis leaf extract, 1 part Liquidambar formosana extract, 1 part Zanthoxylum bungeanum extract, 3 parts Ampelopsis japonica leaf extract and 2 parts Ylang-ylang leaf extract.

[0078] The preparation method of the Ampelopsis grossedentata leaf extract is as follows:

[0079] (1) Wash the fresh Amaryllis leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain Amaryllis leaf powder; (2) Mix the Amaryllis leaf powder and extract at a ratio of 1g:8mL, microwave them, centrifuge them to obtain liquid phase 1, freeze-concentrate them, centrifuge them again to obtain liquid phase 2, and freeze-dry them to obtain Amaryllis leaf extract.

[0080] The extract, by mass percentage, comprises the following components: 0.015% sodium carbonate, 0.08% disodium ethylenediaminetetraacetate, 0.1% tert-butyl-p-hydroxyanisole, with the balance being water; the pH of the extract is 8.5; the microwave treatment conditions are as follows: power 600W, time 60min; the specific operation for freeze concentration is as follows: liquid phase 1 is placed in an ice bath at -5℃ and stirred at 60rpm for 30min, then cooled to -20℃ at -2℃ / min, maintaining the stirring speed for 60min, and the ice-water mixture is centrifuged to obtain liquid phase 2; the specific operation for freeze drying is as follows: pre-cooled to -60℃, then dried at -20℃ under a vacuum of 20Pa for 24h.

[0081] The preparation method of the ylang-ylang (Cananga odorata) leaf extract is as follows:

[0082] (1) Wash fresh ylang-ylang leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain ylang-ylang leaf powder; (2) Extract the ylang-ylang leaf powder with supercritical CO2 to obtain the first extract and ylang-ylang leaf residue; (3) Mix the ylang-ylang leaf residue with a low eutectic solvent and water, microwave it, centrifuge it, and add sodium hydroxide to the obtained liquid phase to adjust the pH of the second extract to 7.2 to obtain the second extract; (4) Combine the first extract and the second extract, and then freeze-dry them to obtain the ylang-ylang leaf extract.

[0083] The supercritical CO2 extraction conditions were as follows: extraction pressure of 38 MPa, extraction temperature of 50℃, extraction time of 120 min, and supercritical CO2 flow rate of 25 kg / h. The eutectic solvent consisted of histidine, glycerophosphate, and glycerol in a molar ratio of 7:2:9. The eutectic solvent was prepared by heating glycerol to 85℃, then adding histidine and glycerophosphate, stirring and maintaining the temperature until the mixture formed a transparent and homogeneous liquid, and then cooling it for later use. The mass ratio of ylang-ylang leaf residue to the eutectic solvent and water was 3 g:10 g:10 g. The microwave treatment conditions were as follows: power of 500 W, temperature of 55℃, and time of 30 min. The freeze-drying operation was as follows: pre-cooling to -60℃, then drying at -20℃ under a vacuum of 20 Pa for 24 h.

[0084] Example 7

[0085] This embodiment provides a hair loss prevention and hair care composition, comprising the following raw materials in parts by weight: 6 parts Polygonum multiflorum extract, 3 parts Polygonatum sibiricum extract, 6 parts Platycladus orientalis leaf extract, 2 parts Liquidambar formosana extract, 2 parts Zanthoxylum bungeanum extract, 2 parts Ampelopsis japonica leaf extract and 4 parts Ylang Ylang leaf extract.

[0086] The preparation method of the Ampelopsis grossedentata leaf extract is as follows:

[0087] (1) Wash the fresh Amaryllis leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain Amaryllis leaf powder; (2) Mix the Amaryllis leaf powder and extract, microwave them, centrifuge them to obtain liquid phase 1, freeze-concentrate them, centrifuge them again to obtain liquid phase 2, and freeze-dry them to obtain Amaryllis leaf extract.

[0088] The extract, by mass percentage, comprises the following components: 0.07% sodium carbonate, 0.1% disodium ethylenediaminetetraacetate, 0.3% sodium sulfite, with the remainder being water; the pH of the extract is 8.5; the microwave treatment conditions are as follows: power 800W, time 30min; the specific operation for freeze concentration is as follows: liquid phase 1 is placed in an ice bath at -5℃ and stirred at 60rpm for 30min, then cooled to -20℃ at -2℃ / min, maintaining the stirring speed for 60min, and the ice-water mixture is centrifuged to obtain liquid phase 2; the specific operation for freeze drying is as follows: pre-cooled to -60℃, then dried at -20℃ under a vacuum of 20Pa for 24h.

[0089] The preparation method of the ylang-ylang (Cananga odorata) leaf extract is as follows:

[0090] (1) Wash fresh ylang-ylang leaves, dry them in a forced-air dryer at 40°C for 24 hours, and crush them to 16 mesh to obtain ylang-ylang leaf powder; (2) Extract the ylang-ylang leaf powder with supercritical CO2 to obtain the first extract and ylang-ylang leaf residue; (3) Mix the ylang-ylang leaf residue with a low eutectic solvent and water, sonicate it, centrifuge it, and add sodium hydroxide to the obtained liquid phase to adjust the pH of the second extract to 7.2 to obtain the second extract; (4) Combine the first extract and the second extract, and then freeze-dry them to obtain the ylang-ylang leaf extract.

[0091] The supercritical CO2 extraction conditions were as follows: extraction pressure of 45 MPa, extraction temperature of 40℃, extraction time of 40 min, and supercritical CO2 flow rate of 25 kg / h. The eutectic solvent consisted of histidine, glycerophosphate, and glycerol in a molar ratio of 7:2:9. The eutectic solvent was prepared by heating glycerol to 85℃, then adding histidine and glycerophosphate, stirring and maintaining the temperature until the mixture formed a transparent and homogeneous liquid, and then cooling it for later use. The mass ratio of ylang-ylang leaf residue to the eutectic solvent and water was 3 g:20 g:10 g. The ultrasonic treatment conditions were as follows: power of 500 W, frequency of 30 kHz, and intermittent operation (4 seconds on, 3 seconds off) for a total time of 40 min. The freeze-drying operation was as follows: pre-cooling to -60℃, then drying at -20℃ under a vacuum of 20 Pa for 24 h.

[0092] Comparative Example 1

[0093] The present invention provides a plant extract that differs from Example 1 in that it does not contain Ampelopsis grossedentata leaf extract.

[0094] Comparative Example 2

[0095] This invention provides a plant extract that differs from Example 1 in that it uses Sophora flavescens extract instead of Ampelopsis grossedentata leaf extract. The Sophora flavescens extract is specifically a dried root extract, purchased from Shanyang Lianfeng Biotechnology Co., Ltd., and is in solid powder form, extracted by alcohol and water immersion.

[0096] Test Example 1: In vitro antioxidant performance test

[0097] The compositions in each specific embodiment were dissolved in ultrapure water to obtain a 100 mg / L composition solution. The DPPH free radical scavenging ability assay kit (purchased from Shanghai Enzyme-Link Biotechnology Co., Ltd.) was used, and the experiment was conducted according to the kit instructions. A control group, experimental group, positive group, and blank group were set up, and the test solutions were prepared according to the groupings shown in Table 1 below. The absorbance value of each tube was measured at a wavelength of 517 nm. A 100 mg / L vitamin C solution was used as a positive control in this experiment. The DPPH free radical scavenging rate of each sample was calculated according to the following formula: DPPH free radical scavenging rate (%) = [1 - (A1 - A2) ÷ A...] 空白组 ]×100%, where A1 is the absorbance value of the experimental group or positive group, A2 is the absorbance value of the experimental group control or positive group control, A 空白组 The absorbance values ​​are for the blank tubes. The average DPPH radical scavenging rates of the compositions in each example are shown in Table 2.

[0098] Table 1

[0099]

[0100] Table 2

[0101]

[0102] Two-tailed tests were used for statistical analysis, and independent samples t-tests were used to calculate p-values. The p-values ​​were compared with the model control. *: p < 0.05 indicates a significant difference, and **: p < 0.01 indicates a highly significant difference.

[0103] As shown in Table 2, the compositions provided by the present invention have good antioxidant effects, especially Example 1, whose antioxidant effect is close to that of vitamin C. The antioxidant effect can protect the scalp and hair follicles from damage caused by "oxidative stress", thereby creating a healthier and more stable environment for hair growth.

[0104] Test Example 2: In vitro anti-inflammatory activity test

[0105] HaCaT cells were seeded in well plates and cultured in DMEM medium at 37°C under CO2 conditions. The medium was changed every 48 hours until the cell confluence reached 80% or higher. The cells were then divided into the following groups: blank group, model group, experimental group, and positive control group. Except for the blank group, each group was treated with 1 μg / mL LPS solution for 24 hours. The model group was then treated with normal medium, the experimental group with medium containing a 100 mg / L solution, and the positive control group with medium containing 100 mg / L dexamethasone. Cultures were continued for another 24 hours. The supernatant was then collected, and the concentrations of pro-inflammatory factors TNF-α and IL-1β in the supernatant were detected using an ELISA kit. The results are shown in Table 3.

[0106] Table 3

[0107]

[0108] Two-tailed tests were used for statistical analysis, and independent samples t-tests were used to calculate p-values. The p-values ​​were compared with the model control. *: p < 0.05 indicates a significant difference, and **: p < 0.01 indicates a highly significant difference.

[0109] As shown in Table 3, the composition provided by the present invention has good anti-inflammatory effects. In particular, Example 1 has an antioxidant effect similar to that of dexamethasone. The anti-inflammatory components can break the vicious cycle of "stimulation → inflammation → hair follicle damage → hair loss → more susceptible to stimulation", creating a healthy microenvironment for hair follicles that is free from inflammation and low in oxygen stress, enabling them to carry out normal metabolism and grow vigorously.

[0110] Test Example 3: In vitro inhibition of 5α-reductase activity test

[0111] (1) Six male SD rats were fasted overnight and then sacrificed. Prostate tissue was taken and Tris-HCl buffer (pH=7) at 4℃ was added at a solid-liquid ratio of 1:5. The mixture was centrifuged at 4℃ and 3000g for 10 min. The supernatant was centrifuged at 4℃ and 10000g for 30 min to obtain crude 5α-reductase extract.

[0112] (2) Set up blank group, experimental group, positive control group and reaction group; the reaction of the experimental group is as follows: 500 μL phosphate buffer (pH 5.5, 0.1M), 200 μL of 0.25 mg / mL composition solution, 200 μL of 300 mg / L testosterone solution and 200 μL of 0.8 g / L reduced coenzyme II (NADPH) solution are placed in a stoppered test tube, and finally 0.5 mL of the above 5α-reductase crude extract is added. The reaction is carried out at 37℃ for 30 min. After the reaction is completed, 3 mL of dichloromethane is added to stop the reaction. The mixture is centrifuged at 5000 r / min for 10 min, the upper aqueous phase is discarded, the residue is dissolved in 1 mL of methanol, and 10 μL is taken to determine the residual testosterone content by high performance liquid chromatography. The positive control group reacted the same as the experimental group, except that the composition solution was replaced with 0.05 mg / L finasteride solution; the blank group reacted the same as the experimental group, except that the crude 5α-reductase extract was replaced with an equal volume of phosphate buffer; the reaction group reacted the same as the experimental group, except that the composition solution was replaced with an equal volume of phosphate buffer. The relative peak area S of testosterone was determined by high-performance liquid chromatography (HPLC), and the inhibitory activity was calculated using the following formula: Inhibition rate / % = (S / %) 实验组 / 阳性对照组 -S 反应组 ) / (S 空白组 -S 反应组 The result is shown in Table 4, calculated as 100% × 100%.

[0113] Table 4

[0114]

[0115] As shown in Table 4, the compositions provided by the present invention have good 5α-reductase inhibitory activity, especially Example 1, whose activity is close to that of finasteride, and can reduce the potential for testosterone to convert to DHT and reduce hair follicle atrophy.

[0116] Test Example 4: In vitro antibacterial activity test

[0117] The antibacterial activity of the composition was tested using the Oxford cup method, and the specific procedure was as follows: a single colony of Malassezia was picked and inoculated into Dixon liquid medium, and cultured at 37°C in a shaker for 48 hours until the bacterial concentration reached 1×10⁻⁶. 6 CFU / mL, 100 μL of bacterial suspension was evenly spread on Dixon agar plates; sterile Oxford cups (6 mm inner diameter) were placed in the plates, and 200 μL of 0.25 mg / mL combination solution or positive control drug ketoconazole was added to each cup; after incubation at 37℃ for 72 h, the diameter of the inhibition zone was measured with calipers (accurate to 0.1 mm). The results are shown in Table 5.

[0118] Table 5

[0119]

[0120] As shown in Table 5, the compositions provided by the present invention have good antibacterial activity, especially Example 1, whose activity is close to that of ketoconazole. It can maintain the healthy balance of the scalp microecology and eliminate hair follicle damage and hair loss caused directly or indirectly by excessive proliferation of microorganisms.

[0121] Test Example 5: In vitro cell viability test

[0122] Human keratinocytes (HaCaT) were seeded into 96-well plates and cultured in DMEM medium (containing 10% FBS) at 37°C and 5% CO2 for 24 h. The medium was then replaced with a solution containing 0.25 mg / mL or 0.5 mg / mL of the combination, and cultured for another 24 h. MTT solution at a concentration of 3 mg / mL was added to the wells, and the cells were cultured for another 4 h. The supernatant was then removed, dimethyl sulfoxide was added, and the cells were shaken for 30 min. The absorbance at 540 nm was measured using a microplate reader, and the cell viability was calculated using the following formula: (Experimental group absorbance ÷ Blank group absorbance) × 100%. The results are shown in Table 6.

[0123] Table 6

[0124]

[0125] As shown in Table 6, the composition provided by the present invention has weak cytotoxicity and also has a certain cell proliferation promoting effect at low concentrations. The ylang-ylang leaf extract used in Example 4 was extracted with ethanol, and it can be seen that the cell survival rate was significantly lower than that of other examples, indicating that ethanol extraction is not selective and extracts the toxic components along with the ethanol.

[0126] Test Example 6: Moisturizing Performance Test

[0127] Forty volunteers aged 25-55 years were selected as subjects and randomly divided into four groups, corresponding to Example 1, Example 2, Comparative Example 1, and Comparative Example 2, with 10 participants in each group (5 males and 5 females). Each night, subjects applied 3 mL of a 0.25 mg / mL solution of the composition to their scalp using a scalp applicator, gently massaging for 3-5 minutes until absorbed, for four consecutive weeks. During the trial, subjects shampooed their hair at least three times a week, following their usual routine, but using only the designated shampoo (which contained no active ingredients). The scalp stratum corneum moisture content was measured before and after the trial using a Corneometer CM825. Subjects did not shampoo for 48 hours prior to each test. Before each test, subjects sat quietly for 30 minutes in an indoor environment at 20°C and 50% relative humidity. Measurements were taken three times at a fixed area (near the Baihui acupoint), and the average values ​​were recorded. The skin moisture content results are shown in Table 7.

[0128] Table 7

[0129]

[0130] As shown in Table 7, the compositions provided by this invention have good moisturizing properties for the scalp, especially in Example 1. Higher scalp moisture content is more conducive to strengthening the scalp barrier function, maintaining hair follicle health, and balancing sebum secretion. Furthermore, Example 2 used *Ampelopsis grossedentata* leaf extract extracted by water immersion, Comparative Example 1 did not include *Ampelopsis grossedentata* leaf extract, and Comparative Example 2 used *Sophora flavescens* extract instead of *Ampelopsis grossedentata* leaf extract; both showed weaker moisturizing effects on the scalp.

[0131] Example 8

[0132] This embodiment provides an anti-hair loss hair care essence, which, by weight percentage, comprises the following components: 20wt% of the anti-hair loss hair care composition of Example 1, 2wt% octyl / decyl glucoside, 1wt% sodium dodecylbenzenesulfonate, 0.05wt% phenoxyethanol, 5wt% 1,3-propanediol, 2wt% panthenol, 8wt% glycerin, 0.032wt% citric acid, and the balance being water.

[0133] The preparation method of the above-mentioned anti-hair loss and hair care essence includes the following steps:

[0134] Octyl / decyl glucoside, sodium dodecylbenzenesulfonate, phenoxyethanol, 1,3-propanediol, panthenol, glycerin, and water were mixed evenly at 60°C to obtain a premix. The premix was cooled to 40°C, and the anti-hair loss and hair care composition was added and stirred evenly. Finally, a pH adjuster was added to adjust the pH to 6.8 and defoaming was performed to obtain the anti-hair loss and hair care essence.

[0135] Comparative Example 3

[0136] This embodiment provides an essence comprising, by weight percentage: 20 wt% of the composition of Comparative Example 2, 2 wt% of octyl / decyl glucoside, 1 wt% of sodium dodecylbenzenesulfonate, 0.05 wt% of phenoxyethanol, 5 wt% of 1,3-propanediol, 2 wt% of panthenol, 8 wt% of glycerol, 0.032 wt% of citric acid, with the balance being water.

[0137] The preparation method of the above-mentioned essence includes the following steps:

[0138] Octyl / decyl glucoside, sodium dodecylbenzenesulfonate, phenoxyethanol, 1,3-propanediol, panthenol, glycerin and water were mixed evenly at 60°C to obtain a premix; the premix was cooled to 40°C, and the composition of Comparative Example 2 was added and stirred evenly. Finally, a pH adjuster was added to adjust the pH to 6.8 and defoaming was performed to obtain an anti-hair loss and hair care essence.

[0139] Test Example 7: In vivo anti-hair loss and hair strengthening effect test

[0140] Twenty-four 6-week-old SPF-grade female C57BL / 6 mice, weighing 18g-20g, were divided into three groups: a saline group, Example 8 group, and Comparative Example 3 group. Mice were anesthetized by intraperitoneal injection of sodium pentobarbital. A 2cm × 4cm area was selected on the back and tail of the mice for hair removal. Longer hairs were shaved using an electric razor, and then human depilatory cream was evenly applied to the area. After 10 minutes of inactivity, the depilatory cream and mouse hair were wiped off with gauze, and the area was rinsed with saline. The day after hair removal, the corresponding substance was applied to the area. The saline group received an equal amount of saline (0.4mL / mouse). The day of application was designated as Day 1. The same treatment was administered at the same time each day. On Day 10 and Day 20, three mice were randomly selected for cervical dislocation and euthanasia. A 2cm × 2cm section of skin from the treated area was fixed in a tissue fixative and prepared for pathological sectioning. The dermal layer thickness (distance between the basement membrane and the sarcolemma) was observed under a microscope. Figure 1 The image shows the growth of fur on the backs of mice on days 3, 5, and 12 after drug administration. Figure 2The images show pathological sections of mouse skin on days 10 and 20 after drug administration. As can be seen from the images, the hair growth rate in Example 8 and Comparative Example 3 was higher than that in the saline group. Pathological sections show that the epidermis and subcutaneous hair follicles and sebaceous glands in Example 8 had clear structures, regular arrangement of subcutaneous tissue cells, and a thicker stratum corneum compared to Comparative Example 3 and the saline group. This is beneficial for making hair follicles more stable and less prone to atrophy, which helps reduce hair loss.

[0141] In summary, the present invention provides an anti-hair loss and hair care composition, which has good antioxidant, anti-inflammatory, DHT inhibition and antibacterial effects, and exhibits low cytotoxicity; the anti-hair loss and hair care essence provided by the present invention can promote hair growth and thicken the dermal layer in mice.

[0142] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A hair loss prevention and hair care composition, characterized in that, The hair loss prevention and hair care composition comprises the following raw materials in parts by weight: 4-6 parts Polygonum multiflorum extract, 3-6 parts Polygonatum sibiricum extract, 4-6 parts Platycladus orientalis leaf extract, 1-2 parts Liquidambar formosana extract, 1-2 parts Zanthoxylum bungeanum extract, 2-3 parts Ampelopsis japonica leaf extract and 2-4 parts Ylang Ylang leaf extract. The preparation method of the *Ampelopsis grossedentata* leaf extract includes the following steps: (1) washing fresh *Ampelopsis grossedentata* leaves, drying them first, and then pulverizing them to obtain *Ampelopsis grossedentata* leaf powder; (2) mixing the *Ampelopsis grossedentata* leaf powder with the extract, performing ultrasonic treatment and / or microwave treatment, separating the solid and liquid phases, and drying the liquid phase a second time to obtain *Ampelopsis grossedentata* leaf extract; the extract, calculated by mass percentage, includes the following components: 0.01%~0.1% sodium carbonate, 0.05%~0.1% disodium ethylenediaminetetraacetate, 0.1%~0.3% antioxidant, with the remainder being water; the antioxidant is selected from at least one of sodium sulfite, ascorbic acid, and tert-butyl-p-hydroxyanisole; The preparation method of the ylang-ylang leaf extract includes the following steps: (1) Wash, dry and crush fresh ylang-ylang leaves to obtain ylang-ylang leaf powder; (2) Extract the ylang-ylang leaf powder with supercritical CO2 to obtain a first extract and ylang-ylang leaf residue; (3) Mix the ylang-ylang leaf residue with a eutectic solvent and water, and treat it with ultrasound and / or microwave. After centrifugation, add acid or alkali to the obtained liquid phase to adjust the pH of the second extract to 6.8-7.2 to obtain the second extract; (4) Combine the first extract and the second extract, and then freeze-dry to obtain ylang-ylang leaf extract; The eutectic solvent includes histidine, glycerophosphate and glycerol in a molar ratio of 7:2:9; The preparation method of the eutectic solvent is as follows: heat glycerol to 80-90℃, then add histidine and glycerophosphate, keep warm and stir until the mixture forms a transparent and homogeneous liquid, and cool it down for later use.

2. The anti-hair loss and hair care composition according to claim 1, characterized in that, The method for preparing the *Ampelopsis grossedentata* leaf extract satisfies at least one of the following conditions: (1) The first drying process is selected from blower drying or freeze drying; (2) The second drying The process includes the following steps: placing the liquid phase in an ice bath at -5~0℃ and stirring at 30~100 rpm for 30 min, then cooling it to -20℃ at -1~-5℃ / min while maintaining the stirring speed for 60 min, centrifuging to obtain a second liquid phase, pre-cooling it to -60~-40℃, and then drying it at -20~-10℃ under a vacuum of 20~50 Pa for 24~48 h; (3) The pH value of the extract is 8~10; (4) The mixing ratio of the Viburnum odorata leaf powder and the extract is 1g: 3~10mL; (5) The ultrasonic treatment has a power of 200~600W, a frequency of 20~40kHz, a time of 30~60min, and a temperature of 20~40℃; (6) The power of the microwave processing is 500~1000W, the time is 30min, and the temperature is 20~50℃.

3. The anti-hair loss and hair care composition according to claim 1, characterized in that, The method for preparing the ylang-ylang leaf extract satisfies at least one of the following conditions: (1) The supercritical CO2 extraction pressure is 35~45MPa, the extraction temperature is 35~50℃, the extraction time is 30~120min, and the supercritical CO2 flow rate is 20~30kg / h; (2) The mass ratio of the ylang-ylang leaf residue to the eutectic solvent and water is 3~4:10~20:10; (3) The acid is citric acid or acetic acid; the base is sodium hydroxide; (4) The specific operation of the freeze drying is as follows: pre-cool to -60 ~ -40℃, and then dry at -20 ~ -10℃. The vacuum degree of drying is 20~50Pa and the time is 24~48h.

4. A hair loss prevention and conditioning essence, characterized in that, Includes 10wt% to 50wt% of the anti-hair loss and hair care composition according to any one of claims 1 to 3.

5. The anti-hair loss and hair care essence according to claim 4, characterized in that, The hair loss prevention and hair care essence comprises the following components by weight percentage: 10wt%~30wt% hair loss prevention and hair care composition, 1wt%~5wt% surfactant, 0.05wt%~0.1wt% preservative, 10wt%~30wt% moisturizer, 0.02wt%~0.1wt% pH adjuster, and the balance being water.

6. The method for preparing the anti-hair loss and hair care essence according to claim 5, characterized in that, The process includes the following steps: mixing surfactants, humectants, preservatives, and water at 60-65℃ to obtain a premix; cooling the premix to 40-45℃, adding the anti-hair loss and hair care composition and stirring evenly; finally, adding a pH adjuster to adjust the pH to 6.8-7.2 and defoaming to obtain the anti-hair loss and hair care essence.

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