Skin-tightening anti-wrinkle essence and preparation process thereof

Abstract

The invention discloses a skin-tightening anti-wrinkle essence and a preparation process thereof, and relates to the technical field of cosmetic preparation, and the preparation process comprises the following steps: obtaining animal umbilical cord tissue, culturing, collecting cell culture supernatant after culture, carrying out low-speed centrifugation to remove cells and dead cells, and carrying out concentration, filtration and sterilization through a tangential flow membrane technology to obtain the skin-tightening anti-wrinkle essence. The animal umbilical cord extract is obtained; the preparation method comprises the following steps: mixing the animal umbilical cord extract with water, butanediol, glycerol, hydroxypropyl tetrahydropyrantriol, 1, 2-hexanediol, trehalose, p-hydroxyacetophenone, allantoin, hydrolyzed sodium hyaluronate, sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid, xanthan gum and essence, homogenizing under a stirring condition, and standing for defoaming. By constructing an active stable microenvironment and an efficient transdermal delivery system, the technical problem that the animal umbilical cord extract serving as a core component is easy to inactivate and difficult to absorb is solved, and a faster and more stable skin structure remodeling effect is realized by utilizing the synergistic effect of the animal umbilical cord extract and functional components.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetic preparation, in particular to a skin tightening and anti-wrinkle essence and a preparation process thereof. BACKGROUND

[0002] Animal umbilical cord extract, whose core component is exosome, is a nanoscale vesicle secreted by stem cells. As a messenger between cells, it encapsulates various bioactive molecules such as proteins, ribonucleic acids and deoxyribonucleic acids inside, showing great application potential in the fields of life science and beauty technology. Its mechanism of action lies in delivering these active substances to damaged or aged cells, effectively regulating cell function and supplementing cell content, thereby initiating the self-repair and regeneration mechanisms of the skin. Studies have shown that animal umbilical cord extract can effectively promote the proliferation and migration of fibroblasts, stimulate the synthesis of collagen and elastin, thereby achieving the purpose of lightening wrinkles and tightening skin. In addition, it can also show positive improvement on sensitive skin, acne marks, dull skin and other skin problems through repairing damaged skin barrier, inhibiting inflammatory response and improving microcirculation, and can enhance the overall immunity of the skin and achieve the effect of delaying aging from the source.

[0003] Although animal umbilical cord extract has excellent skin care effects, its large-scale application in the prior art still faces two core technical bottlenecks. First, the stability of its biological activity, as a biological vesicle with a lipid bilayer membrane structure, animal umbilical cord extract is extremely sensitive and fragile in conventional cosmetic matrices. Changes in environmental pH, incompatible solvents or chemical shocks from traditional preservatives can easily cause the vesicle structure to rupture, release and inactivate the active factors encapsulated inside, thereby losing its intended biological function. Second, the obstacle of its transdermal absorption efficiency, the stratum corneum, as the outermost physical barrier of the skin, naturally hinders the penetration of exogenous macromolecular substances. Although the particle size of animal umbilical cord extract is in the nanometer range, it is still a macromolecule relative to the intercellular space of the stratum corneum, making it difficult to effectively penetrate the barrier and reach the target cells in the deep epidermis and dermis. Therefore, although there are related concept products on the market, most of them fail to solve the above-mentioned core problems of stability and absorption efficiency, resulting in a significant reduction in actual effectiveness and the inability to fully realize the potential of the active substance. SUMMARY

[0004] The purpose of the present application is to provide a skin tightening and anti-wrinkle essence and a preparation process thereof, which solves the problems in the background art.

[0005] To solve the above technical problems, the present application provides a preparation process of a skin tightening and anti-wrinkle essence, comprising the following steps: (1) Preparation of animal umbilical cord extract: The umbilical cord tissue of an animal is obtained, washed and disinfected, and cut into tissue pieces; the tissue pieces are subjected to adherent culture, and when the cell growth fusion degree reaches 80-90%, trypsin digestion and subculture expansion culture are performed; when the cell fusion degree reaches 80-90% again, the culture medium is replaced with a serum-free culture medium for continued culture, and after the culture is completed, the cell culture supernatant is collected; the collected supernatant is subjected to low-speed centrifugation in sequence to remove cells and dead cells, then concentrated through tangential flow membrane technology, and finally filtered through a microporous filter to remove bacteria, thereby obtaining an animal umbilical cord extract; (2) Preparation of the serum: The animal umbilical cord extract prepared in step (1) is mixed with water, butylene glycol, glycerol, hydroxypropyl tetrahydro pyran triol, 1,2-hexanediol, trehalose, p-hydroxyacetophenone, allantoin, hydrolyzed sodium hyaluronate, sodium hyaluronate, tetrahydro methyl pyrimidine carboxylic acid, xanthan gum and fragrance, and subjected to homogenization treatment under stirring, and then left to stand for defoaming, thereby obtaining a skin firming and anti-wrinkle serum.

[0006] Preferably, the serum is composed of the following components by weight percentage: Water: 80-90%; Butylene glycol: 2-10%; Glycerol: 2-8%; Hydroxypropyl tetrahydro pyran triol: 0-3%; 1,2-Hexanediol: 0-3%; Trehalose: 0-2.5%; P-hydroxyacetophenone: 0-1.5%; Allantoin: 0-2%; Animal umbilical cord extract: 0-2%; Hydrolyzed sodium hyaluronate: 0-1.5%; Sodium hyaluronate: 0-1%; Tetrahydro methyl pyrimidine carboxylic acid: 0-1%; Xanthan gum: 0-0.8%; Fragrance: 0-0.5%.

[0007] Preferably, the adherent culture in step (1) is performed in a 37°C, 5% CO2 incubator.

[0008] Preferably, after the serum-free culture medium is replaced in step (1), the continued culture is performed for 72 hours.

[0009] Preferably, the low-speed centrifugation in step (1) includes: centrifugation at 300g for 10 minutes at 4°C to take the supernatant; and centrifugation at 2000g for 10 minutes to take the supernatant.

[0010] Preferably, the microporous filter used for the bacteria removal filtration in step (1) has a pore size of 0.22 μm.

[0011] Preferably, butanediol in step (2) is compounded with trehalose to build a microenvironment with osmotic pressure and structural stability to maintain the activity of animal umbilical cord extract; 1,2-hexanediol is compounded with p-hydroxyacetophenone to form a mild preservative system.

[0012] Preferably, butanediol in step (2) synergizes with sodium hyaluronate to promote the transdermal absorption of animal umbilical cord extract through hydration and gradient osmosis.

[0013] Preferably, animal umbilical cord extract in step (2) synergizes with hydroxypropyl tetrahydropyrane triol, the former activates cells as a signal transmission, and the latter promotes the synthesis of glycosaminoglycans as a structural material, together realizing skin structure remodeling.

[0014] Also provided is a skin tightening and anti-wrinkle essence prepared by the preparation process of the skin tightening and anti-wrinkle essence.

[0015] Compared with the prior art, the present application has the following beneficial effects: By constructing a unique compound system, a stable and suitable microenvironment is successfully created for the core active ingredient animal umbilical cord extract. The synergistic effect of multiple moisturizing agents and biological protective agents in the formula can form a protective layer on the surface of exosome vesicles, effectively regulate the osmotic pressure balance, and prevent it from being broken and inactivated due to changes in the external environment. At the same time, the mild preservative system used avoids chemical damage to the lipid membrane of the vesicle by traditional preservatives, thereby maximizing the structural integrity and biological activity of the core active ingredient during the entire shelf life of the product, ensuring the stable exertion of product efficacy.

[0016] The synergistic effect of multiple ingredients significantly improves the transdermal absorption efficiency of the macromolecular active substance animal umbilical cord extract. The small molecular alcohol moisturizing agent in the formula can effectively hydrate the stratum corneum, temporarily increase the fluidity of the lipid between keratinocytes, thereby opening the penetration channel for active ingredients. On this basis, the small molecular sodium hyaluronate can penetrate first, establishing a water gradient from the surface to the inside of the skin, using this driving force to guide and carry the umbilical cord extract to the deep layer of the skin, greatly improving its bioavailability, so that it can efficiently act on deep target cells.

[0017] The synergistic effect between various functional components is achieved, and the skin care effect far exceeding the superposition of single component is achieved. The umbilical cord extract of animal as a signal soldier is scientifically compounded with hydroxypropyl tetrahydropyran triol as a structural material. The former is responsible for transmitting biological signals to fibroblasts, instructing them to start the regeneration program and up-regulate the gene expression of collagen; the latter efficiently promotes the synthesis of extracellular matrix, providing sufficient raw materials for the assembly of collagen fibers, so that the remodeling process of skin structure is faster and more stable, and the anti-wrinkle firming effect is also improved in quality. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present application will be described below. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0019] Embodiment 1 The skin firming and anti-wrinkle essence provided by the present embodiment is composed of the following components by weight percentage: water 85.4%, butylene glycol 4%, glycerol 3%, hydroxypropyl tetrahydropyran triol 2%, 1,2-hexanediol 1.5%, trehalose 1%, p-hydroxyacetophenone 0.5%, allantoin 0.5%, animal umbilical cord extract 1%, hydrolyzed sodium hyaluronate 0.5%, sodium hyaluronate 0.3%, tetrahydro methyl pyrimidine carboxylic acid 0.3%, xanthan gum 0.3%, fragrance 0.2%; The preparation process comprises the following steps: First, prepare the animal umbilical cord extract: obtain animal umbilical cord tissue, cut into tissue blocks after washing and disinfection; the tissue blocks are cultured in a 37℃, 5% CO2 incubator, and when the cell growth fusion degree reaches 80-90%, trypsin digestion and subculture expansion culture are carried out; when the cell fusion degree reaches 80-90% again, replace the serum-free medium for continuous culture for 72 hours, and then collect the cell culture supernatant; centrifuge the collected supernatant at 300g for 10 minutes and 2000g for 10 minutes at 4℃ to remove cells and dead cells, then concentrate by tangential flow membrane technology, and finally filter sterilization through 0.22μm microporous filter membrane to obtain the animal umbilical cord extract; Then the essence is prepared: the animal umbilical cord extract prepared above is mixed with other components and homogenized under stirring, and left to stand to remove bubbles; in this process, the animal umbilical cord extract is constructed with an osmotic pressure and a structure-stable microenvironment by compounding butanediol, glycerol and trehalose, effectively maintaining its biological activity; at the same time, the compounding of 1,2-hexanediol and p-hydroxyacetophenone constitutes a mild preservative system; the synergistic effect of butanediol and sodium hyaluronate promotes the transdermal absorption of the animal umbilical cord extract through hydration and gradient permeation; when the obtained essence is applied to skin external use products, the animal umbilical cord extract activates cells as a signal transmission material, and hydroxypropyl tetrahydropyranyl triol promotes the synthesis of glycosaminoglycans as a structural raw material, and the two synergistically work together to achieve skin structure remodeling, achieving the effect of tightening the skin and lightening wrinkles.

[0020] Example 2 The present embodiment provides a skin tightening and anti-wrinkle essence, which is composed of the following components by weight percentage: water 82.5%, butanediol 5%, glycerol 4%, hydroxypropyl tetrahydropyranyl triol 1.5%, 1,2-hexanediol 2%, trehalose 1.5%, p-hydroxyacetophenone 0.8%, allantoin 0.5%, animal umbilical cord extract 2%, sodium hyaluronate 1%, sodium hyaluronate 0.5%, tetrahydro methyl pyrimidine carboxylic acid 0.5%, xanthan gum 0.5%, fragrance 0.2%; The preparation process is the same as that of Example 1; the preparation conditions of the animal umbilical cord extract include adherent culture in a 37°C, 5% CO2 incubator, serum-free medium culture for 72 hours, low-speed centrifugation at 300g and 2000g at 4°C, and bacteria removal by 0.22μm microporous filter membrane; the formula design of the present embodiment aims to strengthen the core role of the animal umbilical cord extract; a higher concentration of animal umbilical cord extract as a key to signal transmission can more effectively activate cells, and its contained miRNA and growth factors can transmit instructions to fibroblasts, significantly up-regulating the gene expression of collagen and elastin; when the essence prepared by the process is applied to skin external use products for repairing sensitive skin or lightening skin color, it can play an excellent endogenous repair effect, inducing damaged cells to start the self-repair program and reprogramming to a healthy functional state through information exchange.

[0021] Example 3 The present embodiment provides a skin tightening and anti-wrinkle essence, which is composed of the following components by weight percentage: water 82.9%, butanediol 6%, glycerol 3%, hydroxypropyl tetrahydropyranyl triol 3%, 1,2-hexanediol 1%, trehalose 2%, p-hydroxyacetophenone 0.6%, allantoin 0.5%, animal umbilical cord extract 0.5%, sodium hyaluronate 1%, sodium hyaluronate 0.8%, tetrahydro methyl pyrimidine carboxylic acid 0.5%, xanthan gum 0.4%, fragrance 0.3%; The preparation process is the same as that of Example 1; this example focuses on the role of hydroxypropyl tetrahydropyran triol in skin structure remodeling; high content of hydroxypropyl tetrahydropyran triol as a high-efficiency structure material can fully supply the synthesis of glycosaminoglycans required after the activation of animal umbilical cord extract on cell signal; the serum prepared by this process shows the synergistic effect of the complete pathway of instructions and raw materials in the product for tightening skin and lightening wrinkles; animal umbilical cord extract gives orders, and hydroxypropyl tetrahydropyran triol provides support, so as to realize rapid and stable skin structure remodeling, and the tightening effect is more prominent than that of the formula which depends on signal activation alone.

[0022] Example 4 The skin tightening and anti-wrinkle serum provided by the present example is composed of the following components by weight percentage: water 80%, butanediol 8%, glycerol 5%, hydroxypropyl tetrahydropyran triol 2.5%, 1,2-hexanediol 2.5%, trehalose 2.5%, p-hydroxyacetophenone 1%, allantoin 1%, animal umbilical cord extract 1.5%, hydrolyzed sodium hyaluronate 1.5%, sodium hyaluronate 1%, tetrahydro methyl pyrimidine carboxylic acid 1%, xanthan gum 0.8%, fragrance 0.2%; The preparation process is the same as that of Example 1; this formula aims to explore the comprehensive effect of high-concentration complex of each functional component; the synergy of butanediol and high-concentration hydrolyzed sodium hyaluronate creates efficient transdermal absorption conditions for macromolecular animal umbilical cord extract; at the same time, the high-concentration trehalose, glycerol and butanediol complex system provides a stable microenvironment for the activity of high-value animal umbilical cord extract; the serum obtained by this process performs comprehensively in skin external products for lightening skin color and repairing sensitive skin; the multi-target system constructed by animal umbilical cord extract, allantoin and tetrahydro methyl pyrimidine carboxylic acid synergistically inhibits inflammation, repairs damage and improves skin color.

[0023] Example 5 The skin tightening and anti-wrinkle serum provided by the present example is composed of the following components by weight percentage: water 90%, butanediol 2%, glycerol 2%, hydroxypropyl tetrahydropyran triol 1%, 1,2-hexanediol 0.5%, trehalose 0.5%, p-hydroxyacetophenone 0.3%, allantoin 0.4%, animal umbilical cord extract 0.5%, hydrolyzed sodium hyaluronate 0.5%, sodium hyaluronate 0.5%, tetrahydro methyl pyrimidine carboxylic acid 0.3%, xanthan gum 0.2%, fragrance 0.1%; The preparation process is the same as that of Example 1; this formula is a basic efficacy type, the concentrations of the active ingredients are low, but the core technical framework of the application is completely retained; the technologies of synergistically promoting the absorption of animal umbilical cord extract by butanediol and sodium hyaluronate hydrolyzate, and synergistically remodeling the skin structure by animal umbilical cord extract and hydroxypropyl tetrahydropyran triol are applied; the serum prepared by this process has a refreshing skin feel and is suitable for daily maintenance. In skin external products for tightening skin and lightening wrinkles, it can play a basic preventive and improvement effect, and the application of a mild preservative system also ensures the safety of the product.

[0024] Comparative Example 1 The skin tightening and anti-wrinkle serum formula and preparation process of this comparative example are basically the same as those of Example 1, except that animal umbilical cord extract is not added, and its weight is replaced by water.

[0025] Comparative Example 2 The skin tightening and anti-wrinkle serum formula and preparation process of this comparative example are basically the same as those of Example 1, except that hydroxypropyl tetrahydropyran triol is not added, and its weight is replaced by water.

[0026] Comparative Example 3 The skin tightening and anti-wrinkle serum formula and preparation process of this comparative example are basically the same as those of Example 1, except that trehalose and sodium hyaluronate hydrolyzate are not added, and their weights are replaced by water.

[0027] Effect verification To verify the actual effect of the skin tightening and anti-wrinkle serum described in the application, the serums prepared in Examples 1-5 and Comparative Examples 1-3 were subjected to human efficacy testing; 80 healthy female volunteers aged 35-55 were recruited and randomly divided into 8 groups, 10 people in each group; the volunteers used the corresponding test samples on the face after cleansing in the morning and evening every day, for 28 consecutive days; on day 0 and day 28, the wrinkle, tightness, moisture content and redness of the facial skin of the subjects were quantitatively evaluated using professional skin testing instruments, and the activity retention rate of animal umbilical cord extract in the serum was determined; the results were averaged, as shown in the following table: Table 1: Comparison of skin improvement effects of each example and comparative example ; From the data in Table 1, it can be seen that: Examples 1-5 are significantly superior to Comparative Examples 1, 2 and 3 in terms of wrinkle improvement, tightness improvement, moisture content improvement and redness reduction; this indicates that the overall formula design of the application has superior comprehensive efficacy; Comparative Example 1 has a significant decrease in anti-wrinkle, tightening and repair effects due to the lack of the core animal umbilical cord extract, which proves the key role of animal umbilical cord extract as a signal activator; Comparative Example 2 lacks hydroxypropyl tetrahydropyrane triol, although the umbilical cord extract of animals still plays a certain role, but its wrinkle improvement rate and firmness improvement effect is obviously weaker than that of Example 1, which verifies the importance of the synergistic effect of the umbilical cord extract of animals and hydroxypropyl tetrahydropyrane triol; Comparative Example 3 lacks trehalose for maintaining the activity of the extract and sodium hyaluronate for promoting absorption, and the retention rate of the activity of the extract is significantly reduced, which eventually leads to the skin care effects being weaker than those of Example 1, proving that the complex system constructed by the present application for maintaining activity and promoting absorption is necessary and effective; In all examples, Example 4 exhibits the most comprehensive technical effect due to the balanced concentration ratio and high content of each active ingredient, and performs best in each index, fully embodying the advancement and synergistic effect of the technical scheme of the present application.

[0028] The above is only a preferred embodiment of the present application, and does not limit the present application in other forms. Any person skilled in the art can modify or change the above disclosed technical content to equivalent embodiments applied to other fields, but any simple modification, equivalent change and modification made to the above examples without departing from the technical scheme of the present application according to the technical essence of the present application.

Claims

1. A process for the preparation of a skin tightening anti-wrinkle serum, characterized in that, The preparation method comprises the following steps: (1) preparing animal umbilical cord extract: Obtain animal umbilical cord tissue, cut it into tissue blocks after washing and disinfecting; carry out adherent culture on the tissue blocks, and carry out trypsin digestion and subculture expansion culture after the cells grow to 80-90% confluence; replace the culture medium with serum-free medium when the cell confluence reaches 80-90% again, continue to culture, and collect the cell culture supernatant after the culture is completed; the collected supernatant is sequentially subjected to low-speed centrifugation to remove cells and dead cells, then concentrated by tangential flow membrane technology, and finally filtered by a microporous filter to remove bacteria, thereby obtaining the animal umbilical cord extract; (2) preparing the essence: Mix the animal umbilical cord extract prepared in step (1), water, butylene glycol, glycerol, hydroxypropyl tetrahydropyranyl triol, 1,2-hexanediol, trehalose, p-hydroxyacetophenone, allantoin, hydrolyzed sodium hyaluronate, sodium hyaluronate, tetrahydro methyl pyrimidine carboxylic acid, xanthan gum and essence, and homogenize under stirring, and then stand for defoaming, thereby obtaining the skin firming and anti-wrinkle essence.

2. The preparation process of the skin tightening anti-wrinkle essence according to claim 1, characterized in that, The skin firming and anti-wrinkle essence is prepared from the following components by weight percentage: Water: 80-90%; Butylene glycol: 2-10%; Glycerol: 2-8%; Hydroxypropyl tetrahydropyranyl triol: 0-3%; 1,2-hexanediol: 0-3%; Trehalose: 0-2.5%; P-hydroxyacetophenone: 0-1.5%; Allantoin: 0-2%; Animal umbilical cord extract: 0-2%; Hydrolyzed sodium hyaluronate: 0-1.5%; Sodium hyaluronate: 0-1%; Tetrahydro methyl pyrimidine carboxylic acid: 0-1%; Xanthan gum: 0-0.8%; Essence: 0-0.5%.

3. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, The adherent culture in step (1) is carried out in a 37°C, 5% CO2 incubator.

4. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, After replacing the culture medium with serum-free medium in step (1), the time for continuing to culture is 72 hours.

5. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, The low-speed centrifugation in step (1) comprises: centrifuging at 300g for 10 minutes at 4°C, and taking the supernatant; centrifuging at 2000g for 10 minutes, and taking the supernatant.

6. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, The microporous filter used for filtering and sterilizing in step (1) has a pore size of 0.22 μm.

7. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, The butylene glycol, glycerol and trehalose in step (2) are compounded to construct a microenvironment with osmotic pressure and structural stability to maintain the activity of the animal umbilical cord extract; the 1,2-hexanediol and p-hydroxyacetophenone constitute a mild preservative system.

8. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, The butylene glycol and hydrolyzed sodium hyaluronate in step (2) synergistically promote the transdermal absorption of the animal umbilical cord extract through hydration and gradient penetration.

9. The preparation process of the skin tightening anti-wrinkle serum according to claim 1, characterized in that, The animal umbilical cord extract and hydroxypropyl tetrahydropyranyl triol in step (2) synergistically activate cells as a signal transmission and promote the synthesis of glycosaminoglycans as a structural material, thereby jointly realizing skin structure remodeling.

10. A skin firming and anti-wrinkle essence prepared by the preparation process of the skin firming and anti-wrinkle essence according to any one of claims 1-9.