Nutritional composition capable of resisting oxidation and delaying senescence as well as tablet and preparation method thereof

Abstract

The invention provides a nutritional composition capable of resisting oxidation and delaying senescence. The nutritional composition is prepared from beta-nicotinamide mononucleotide, coenzyme Q10, a plant extract, B vitamins and process auxiliary materials, the process auxiliary materials consist of microcrystalline cellulose, sodium carboxymethyl starch, low-substituted hydroxy propyl cellulose, polyvinylpolypyrrolidone and magnesium stearate. The invention further provides a tablet containing the nutritional composition. The tablet is composed of a polishing agent and a coating tablet. The coated tablet comprises an enteric coating substance and a tablet core prepared from an anti-oxidation and anti-aging nutritional composition. The invention also provides a preparation method of the tablet. According to the nutritional composition disclosed by the invention, high-activity substances, namely the beta-nicotinamide mononucleotide and the coenzyme Q10, are systematically integrated with the plant extract, so that the effects of the beta-nicotinamide mononucleotide and the coenzyme Q10 on the aspects of cell kernel metabolism regulation, energy supply and the like are exerted; the advantages of plant extracts in the aspects of oxidation resistance, inflammation resistance, immune regulation and cell protection are utilized, and a synergistically enhanced anti-aging effect is formed.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of nutritional compositions, and particularly relates to a nutritional composition for resisting oxidation and delaying aging, tablets thereof and a preparation method. BACKGROUND

[0002] With the intensification of population aging trend and the general improvement of health awareness, the research and development of anti-aging and antioxidant nutritional products has been increasingly widely concerned. Nicotinamide and its derivatives (such as β-nicotinamide mononucleotide, NMN) and B vitamins, as key cofactors of cellular energy metabolism and redox reactions, play a significant role in delaying cell aging, maintaining mitochondrial function, etc. Among them, nicotinamide, as the precursor of NAD+ (nicotinamide adenine dinucleotide), actively participates in the core anti-aging mechanisms such as DNA repair and sirtuins signaling pathway regulation; and B vitamins, as the coenzyme of various enzymes, are widely involved in important physiological processes such as energy metabolism, neural regulation and immune response.

[0003] However, the use of single components still has significant limitations. Nicotinamide, coenzyme Q10 and B vitamins can regulate intracellular metabolism, but have weak effects on the structural integrity of cell membranes and the stability of extracellular microenvironment, and are difficult to comprehensively cope with the aging process of the body under the action of multiple factors. On the other hand, traditional Chinese medicine extracts such as resveratrol in Polygonum cuspidatum and curcumin in turmeric, as well as various plant polysaccharides (such as inulin, polysaccharide from Lycium barbarum, polysaccharide from Jujube, etc.), have excellent antioxidant, anti-inflammatory and immunomodulatory functions, and can provide protection from the periphery of the cell and regulate the overall state of the body. However, the bioavailability of these plant active ingredients is often low, and it is difficult for them to play a synergistic role inside the cell.

[0004] There have been attempts in the prior art to combine vitamins with plant extracts, such as the water-soluble complex antioxidant disclosed in CN110089658A, which achieves water-oil dual-phase antioxidant through the combination of vitamin E, bamboo leaf antioxidant, etc.; CN120501758A proposes to combine nicotinamide, B vitamins and fungal polysaccharide complex to enhance immunity and anti-fatigue ability. However, these solutions still do not fully integrate the advantages of modern biosynthetic high-activity ingredients and traditional medicinal and edible plants, and although the prior art provides a combination idea, it still has obvious defects in terms of regulating NAD+ metabolic pathways, synergistic protection of cell membranes and cell nuclei, and improving systemic bioavailability.

[0005] Therefore, how to scientifically combine NAD+ precursors (such as NMN) and coenzyme Q10 prepared by modern biotechnology with traditional Chinese medicine extracts, and improve their stability and absorption efficiency through formulation technology, has become an important direction for the development of current anti-aging nutritional products. SUMMARY

[0006] The technical problem to be solved by the present application is to provide an antioxidant and anti-aging nutritional composition, tablets and a preparation method thereof, which overcomes the shortcomings of the prior art.

[0007] To solve the above technical problems, the technical scheme adopted by the present application is as follows: an antioxidant and anti-aging nutritional composition comprises the following raw materials: beta-nicotinamide mononucleotide, coenzyme Q10, plant extract, B vitamins and process aids. The process aids are composed of microcrystalline cellulose, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, cross-linked polyvinylpyrrolidone and magnesium stearate.

[0008] Preferably, the composition is composed of the following raw materials in mass fraction: beta-nicotinamide mononucleotide 40%, coenzyme Q10 10%, plant extract 30%, B vitamins 0.75%, and the balance is process aids.

[0009] Preferably, the plant extract is composed of the following raw materials: rhizoma polygoni multiflori extract, curcuma longa extract, jerusalem artichoke and chichory extract, lycium barbarum extract and jujube extract. The rhizoma polygoni multiflori extract and the curcuma longa extract are extracted by ethanol solvent extraction method, and the jerusalem artichoke and chichory extract, lycium barbarum extract and jujube extract are extracted by ultrasonic-assisted hot water extraction method.

[0010] Preferably, the extraction method of the rhizoma polygoni multiflori extract is as follows: 60% to 95% ethanol by volume is used as the solvent, and reflux extraction is carried out at 70 to 80 DEG C for 1 to 3 times, each time for 1 to 2 hours, the extraction liquid is combined, and after concentration and drying, the rhizoma polygoni multiflori extract is obtained, wherein the resveratrol content is not less than 20%. The extraction method of the curcuma longa extract is as follows: 60% to 95% ethanol by volume is used as the solvent, and reflux extraction is carried out at 60 to 80 DEG C for 1 to 3 times, each time for 1 to 2 hours, the extraction liquid is combined, and after concentration and drying, the curcuma longa extract is obtained, wherein the curcumin content is not less than 80%. The curcumin is extracted from plant cells by using the good solubility of organic solvents, and the commonly used solvents are: methanol, ethanol, acetone, ethyl acetate, etc. Among them, ethanol is the preferred solvent in the industrial and food fields due to its safety, non-toxicity and moderate cost. Process flow: Pre-treatment: dry turmeric is crushed.

[0011] Extraction: Multiple extraction with ethanol in a heated reflux apparatus.

[0012] Filtration and concentration: Combine the extracts, filter, and recover the ethanol under reduced pressure to obtain the crude curcumin extract.

[0013] Purification: The crude extract can be further purified by acid / base treatment, crystallization, column chromatography, etc. to obtain high-purity curcumin.

[0014] The extraction method of the chicory and endive extract is as follows: mix the raw material with water at a ratio of 1:(10-20), and extract at 70-85°C with the aid of ultrasonic waves (power 300-500W) for 20-40 minutes. The extract is concentrated, alcohol precipitated, and dried to obtain the product, wherein the inulin content is not less than 80%. Endive / chicory → cleaning → slicing or crushing → drying → pulverizing.

[0015] Hot water extraction (core step): Method: Mix the raw material with water in a certain proportion, and extract at a specific temperature and pH value.

[0016] Key parameters: Temperature: Usually controlled at 70-85°C. Too high a temperature may cause inulin to hydrolyze into monosaccharides, and too low a temperature may result in insufficient extraction rate.

[0017] pH value: Usually performed under slightly acidic to neutral conditions (pH 5.5-7.0) to prevent inulin from hydrolyzing under acidic conditions.

[0018] Time: Multiple extraction, each for about 30-60 minutes.

[0019] The extraction method of the wolfberry extract is as follows: mix the raw material with water at a ratio of 1:(15-25), and extract at 80-95°C with the aid of ultrasonic waves (power 300-500W) for 20-40 minutes. The extract is concentrated, alcohol precipitated, and dried to obtain the product, wherein the wolfberry polysaccharide content is not less than 30%. The extraction method of the jujube extract is as follows: mix the raw material with water at a ratio of 1:(15-25), and extract at 80-95°C with the aid of ultrasonic waves (power 300-500W) for 20-40 minutes. The extract is concentrated, alcohol precipitated, and dried to obtain the product, wherein the jujube polysaccharide content is not less than 25%.

[0020] Preferably, the plant extract is composed of the following mass fractions of raw materials: 14% polygonum cuspidatum extract, 21% turmeric extract, 28% chicory and endive extract, 14% wolfberry extract, and the balance being jujube extract.

[0021] Preferably, the B vitamins are composed of the following raw materials: vitamin B2, vitamin B9, vitamin B12 and vitamin B6.

[0022] Preferably, the B vitamins are composed of the following raw materials in mass fraction: vitamin B2 38%, vitamin B9 10%, vitamin B12 0.15%, and the balance is vitamin B6.

[0023] Preferably, the process auxiliary materials are composed of microcrystalline cellulose, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, cross-linked polyvinylpyrrolidone and magnesium stearate in a mass ratio of 10:5:3:2.

[0024] The application also provides tablets containing the above-mentioned antioxidant and anti-aging nutritional composition, which are prepared from the following raw materials in parts by weight: polishing agent 0.1-0.2 parts, coating tablets 1 part; The coating tablets comprise the following raw materials in parts by weight: enteric coating material 8-10 parts, tablet core made of the antioxidant and anti-aging nutritional composition 100 parts.

[0025] Preferably, the polishing agent is magnesium stearate. The enteric coating material is composed of hypromellose phthalate and polyethylene glycol 6000 in a mass ratio of 4:1.

[0026] The application also provides a method for preparing the above-mentioned tablets, which comprises the following steps: S1, powdering β-nicotinamide mononucleotide, coenzyme Q10, plant extract and B vitamins, and then resting for 20 min, and then passing through a 200-mesh sieve to obtain material a; S2, powdering sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose and magnesium stearate, and then resting for 100 min, and then passing through a 200-mesh sieve to obtain material b; S3, mixing material a obtained in S1 and material b obtained in S2, and then adding microcrystalline cellulose and cross-linked polyvinylpyrrolidone, and then mixing for 30-40 min, and then resting for 10 min to obtain an antioxidant and anti-aging nutritional composition, which is denoted as material c; S4, tabletting material c obtained in S3 to obtain a tablet core made of the antioxidant and anti-aging nutritional composition; S5, adding enteric coating material to deionized water while stirring, and then stirring for 45-60 min, and then resting for 30 min to remove bubbles to obtain an enteric coating liquid; The mass ratio of the enteric coating material to deionized water is 1:8. S6, spray the enteric coating solution obtained in S5 to the tablet core prepared from the anti-oxidation and anti-aging nutritional composition in S4 to obtain a coated tablet; the spraying parameters are as follows: the inlet air temperature is 38-42 DEG C, the material temperature is 32-35 DEG C, the atomization pressure is 0.8-1.2 Bar, and the liquid spraying rate is 10-15 mL / min; S7, continue to dry and solidify the coating film of the coated tablet obtained in S6 at a temperature of 32-35 DEG C for 15-20 min, then add a polishing agent, and polish at a rotation speed of 10-15 rpm for 5-10 min to obtain a tablet.

[0027] Compared with the prior art, the present application has the following advantages: The present application realizes multi-level and multi-functional synergistic anti-oxidation and anti-aging effects by scientifically matching the modern biotechnologically prepared beta-nicotinamide mononucleotide, coenzyme Q10, and traditional "medicinal and edible" plant extracts (polygonum cuspidatum extract, turmeric extract, jerusalem artichoke and chicory extract, and medlar extract) and B vitamins. The composition can effectively improve immune regulation and anti-fatigue ability, and solves the technical problem that a single component is difficult to synergistically regulate intracellular NAD+ level and extracellular antioxidant barrier. By optimizing the enteric coating process, the stability of the active ingredients and the intestinal target release are ensured, and the bioavailability is greatly improved. All raw materials meet the food safety standards, the production process is simple and reliable, and the present application can be widely applied in the fields of health products, functional foods and cosmetics, and has significant health benefits and market application prospects.

[0028] The present application will be further described in detail below in conjunction with examples. DETAILED DESCRIPTION

[0029] Example 1 The anti-oxidation and anti-aging nutritional composition of the present embodiment is composed of the following raw materials in mass fraction: beta-nicotinamide mononucleotide (NMN) 40%, coenzyme Q10 10%, plant extract 30%, B vitamins 0.75%, and the balance is process auxiliary materials; The process auxiliary materials are composed of microcrystalline cellulose (MCC), carboxymethyl starch sodium (CMS-Na), low-substituted hydroxypropyl cellulose (L-HPC), cross-linked polyvinylpyrrolidone (RPM), and magnesium stearate in a mass ratio of 10:5:3:2; The B vitamins are composed of the following raw materials in mass fraction: vitamin B2 (riboflavin) 38%, vitamin B9 (folic acid) 10%, vitamin B12 (cobalamin) 0.15%, and the balance is vitamin B6 (pyridoxine).

[0030] The plant extract is composed of raw materials in the following mass fractions: 14% of polygonum cuspidatum extract, 21% of curcuma longa extract, 28% of jerusalem artichoke and chicory extract, 14% of lycium barbarum extract, and the balance of zizyphus jujuba extract; The extraction method of the polygonum cuspidatum extract is as follows: 60% by volume of ethanol is used as a solvent, and the extraction is performed at 70 DEG C for 3 times, 1 hour each time, the extraction liquid is combined, and after being concentrated and dried, the polygonum cuspidatum extract is obtained, wherein the content of resveratrol is not less than 20%; The extraction method of the curcuma longa extract is as follows: 60% by volume of ethanol is used as a solvent, and the extraction is performed at 60 DEG C for 3 times, 1 hour each time, the extraction liquid is combined, and after being concentrated and dried, the curcuma longa extract is obtained, wherein the content of curcumin is not less than 80%; The extraction method of the jerusalem artichoke and chicory extract is as follows: the raw material is mixed with water at a ratio of 1:10, and the extraction is performed at 70 DEG C for 20 minutes with the aid of ultrasonic waves (power 500 W), the extraction liquid is concentrated, alcohol precipitated, and dried to obtain the jerusalem artichoke and chicory extract, wherein the content of inulin is not less than 80%; Chicory / jerusalem artichoke → cleaning → slicing or crushing → drying → pulverizing.

[0031] Hot water extraction (core step): Method: the raw material is mixed with water in a certain proportion, and the extraction is performed at a specific temperature and pH value.

[0032] Key parameters: Temperature: usually controlled at 70 DEG C to 85 DEG C, too high temperature may lead to the hydrolysis of inulin into monosaccharides, and too low temperature may result in insufficient extraction rate.

[0033] pH value: usually performed under the condition of slightly acidic to neutral (pH 5.5) to prevent the hydrolysis of inulin under acidic condition.

[0034] Time: 3 times of extraction, about 30 minutes each time.

[0035] The extraction method of the lycium barbarum extract is as follows: the raw material is mixed with water at a ratio of 1:15, and the extraction is performed at 80 DEG C to 95 DEG C for 20 minutes with the aid of ultrasonic waves (power 300 W), the extraction liquid is concentrated, alcohol precipitated, and dried to obtain the lycium barbarum extract, wherein the content of lycium barbarum polysaccharide is not less than 30%; The extraction method of the zizyphus jujuba extract is as follows: the raw material is mixed with water at a ratio of 1:15, and the extraction is performed at 80 DEG C for 20 minutes with the aid of ultrasonic waves (power 300 W), the extraction liquid is concentrated, alcohol precipitated, and dried to obtain the zizyphus jujuba extract, wherein the content of zizyphus jujuba polysaccharide is not less than 25%.

[0036] The main component of the polygonum cuspidatum extract is resveratrol, the main component of the curcuma longa extract is curcumin, the main component of the jerusalem artichoke and chicory extract is inulin polysaccharide, the main component of the lycium barbarum extract is lycium barbarum polysaccharide, and the main component of the zizyphus jujuba extract is zizyphus jujuba polysaccharide.

[0037] The anti-oxidation and anti-aging nutritional composition in the embodiment plays a role through a multi-level and multi-target synergistic mechanism of each raw material: Core metabolic regulation (intracellular): β-nicotinamide mononucleotide (NMN) as a key precursor of NAD+, significantly improves intracellular NAD+ levels, thereby activating the Sirtuins longevity protein family, enhancing DNA repair capacity, regulating cell metabolic rhythm, and delaying cell aging from the root.

[0038] Mitochondrial energy and anti-oxidation (organelle level): coenzyme Q10 is an important component of the mitochondrial electron transport chain, which can efficiently promote ATP synthesis and provide energy for cells. At the same time, it is also a fat-soluble antioxidant that can effectively scavenge free radicals produced by mitochondria, protecting mitochondrial function, and forming synergy with NMN in energy metabolism and anti-oxidation.

[0039] Systemic anti-oxidation and anti-inflammation (extracellular and systemic level): plant extract complex provides extensive protection. Polygonum cuspidatum extract (resveratrol) and turmeric extract (curcumin) are potent natural antioxidants and anti-inflammatory agents that can activate antioxidant signaling pathways such as Nrf2 and inhibit inflammatory pathways such as NF-κB. Plant polysaccharides such as inulin, polysaccharide from Fructus Lycii, and polysaccharide from Fructus Jujubae not only can directly scavenge free radicals, but also can regulate gut flora, indirectly playing a role in immune regulation and anti-aging through the "gut-axis".

[0040] Auxiliary factor support: B vitamins as coenzymes of various enzymes, comprehensively support the progress of the above metabolic processes. For example, vitamin B2 is a cofactor for glutathione peroxidase, and vitamins B6, B9, and B12 are involved in homocysteine metabolism, and their levels are closely related to oxidative stress and aging.

[0041] Therefore, the composition not only significantly improves NAD+ levels, enhances mitochondrial function, and reduces oxidative stress damage, but also achieves a systemic anti-aging effect that single components cannot achieve through internal and external synergy, metabolic and immune co-regulation, effectively improving immune regulation and anti-fatigue ability.

[0042] The anti-oxidation and anti-aging nutritional composition in the embodiment not only significantly improves NAD+ (nicotinamide adenine dinucleotide) levels, enhances mitochondrial function, and reduces oxidative stress damage, but also effectively improves immune regulation and anti-fatigue ability, solving the technical problem of single component difficulty in synergistically regulating intracellular NAD+ levels and extracellular antioxidant barrier.

[0043] The embodiment also provides a tablet containing the above anti-oxidation and anti-aging nutritional composition, which is made from the following raw materials by weight: polishing agent (magnesium stearate) 0.2 parts, coated tablet 1 part; The coated tablet comprises the following raw materials by weight: 8 parts of enteric coating material, 100 parts of the tablet core made of the antioxidant, the antioxidant, the nutrient composition for delaying aging; The enteric coating material is composed of hydroxypropyl methyl cellulose phthalate (HPMCP) and polyethylene glycol 6000 (PEG6000) with a mass ratio of 4:1.

[0044] The embodiment also provides a method for preparing the tablet, which comprises the following steps: S1, put β-nicotinamide mononucleotide, coenzyme Q10, plant extracts and B vitamins into a pulverizer to control temperature and refine particles, after pulverization, stand still for 20 min, pass through a 200-mesh sieve to obtain material a; Before use, the β-nicotinamide mononucleotide and coenzyme Q10 in the embodiment are respectively subjected to HPLC analysis, and the purity ≥ 99% is qualified; and the β-nicotinamide mononucleotide and coenzyme Q10 are subjected to vacuum drying at 35°C until the moisture content is ≤ 3% before use; The rhizoma polgoni extract, curcuma longa extract, jerusalem artichoke and chichorium intybus extract, lycium barbarum extract and zizyphus jujuba extract in the plant extracts are weighed according to the proportion (without mixing), and are respectively subjected to vacuum rotary drying at 35°C until the moisture content is ≤ 3% before use; S2, put sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose and magnesium stearate into a powdering machine to control temperature and refine particles, after pulverization, stand still for 100 min, pass through a 200-mesh sieve to obtain material b; The low-substituted hydroxypropyl cellulose (L-HPC) is commercially available and purchased from Jinzhou Hengxiang Chemical Technology Co., Ltd.; S3, put the material a obtained in S1 and the material b obtained in S2 into a V-type mixer to mix, then add microcrystalline cellulose and crosslinked polyvidone to mix for 30 min, stand still for 10 min to obtain the nutrient composition for antioxidant and delaying aging, denoted as material c; S4, tablet the material c obtained in S3 to obtain the tablet core made of the nutrient composition for antioxidant and delaying aging. The tabletting method is as follows: use a preset mold, adjust the parameters of the tablet machine to 40 kN (4 tons), continuously tablet, collect the untablet powder and tablets after tabletting, and count the tablet number n1.

[0045] Q = total mixed powder mass (g) / theoretical tablet number (n1) Control Q: 500 / n1 = 0.45~0.55; If Q < 0.5, a larger size mold needs to be used, if Q > 0.5, a smaller size mold needs to be used, and the substandard tablets can be powdered again using a powdering machine (note that the powdering is already a mixture of material a and material b); this step cannot be omitted, the purpose is to control the content of the main component NMN in the single tablet to be 200 ± 20 mg. Hardness ≤ 5 kg / cm 2 , friability ≤ 0.01 %.

[0046] S5, while stirring, add enteric coating material to deionized water, stir for 45 min until completely dissolved, stand for 30 min to degas, get uniform, translucent enteric coating liquid; The mass ratio of the enteric coating material and deionized water is 1:8; S6, place the tablet core obtained by tabletting into the fluidized bed coating machine, start the equipment, and after the fluidization state and temperature are stable, spray the enteric coating liquid obtained in S5 onto the tablet core made of the antioxidant and anti-aging nutritional composition obtained in S4 to obtain coated tablets; the spraying parameters are: inlet air temperature is 42℃, material temperature is 35℃, atomizing pressure is 0.8 Bar, and liquid spraying rate is 10 mL / min; closely monitor the material temperature and fluidization state during coating to ensure that the coating on the surface of the tablet core is uniform and complete; stop spraying when the weight increase of the tablet core reaches 8% (calculated as dry solid) (i.e. the coated tablets include the following raw materials by weight: enteric coating material 8 parts, tablet core 100 parts); S7, after coating, keep the fluidized state, continue to dry and solidify the coating film of the coated tablets obtained in S6 at a temperature of 32℃ for 15 min to fully solidify the coating film, then transfer into a polishing pot, add polishing agent, and polish at a low speed of 10 rpm for 10 min to make the surface smooth, and obtain the antioxidant and anti-aging nutritional composition tablets.

[0047] S8, quality inspection: randomly sample and test according to the relevant provisions of the Chinese Pharmacopoeia. Key inspection: coating weight gain, tablet appearance (should be smooth surface, uniform color, no defects), and disintegration time limit: use a disintegration tester to test in a phosphate buffer with pH = 7.2 ± 0.2, and it should completely disintegrate within 4-6 h, and there is no hard core after disintegration.

[0048] By preparing the antioxidant and anti-aging nutritional composition of this embodiment into tablets, especially using enteric coating technology, the following significant advantages are achieved: ① Improved stability: The tablet dosage form and coating layer effectively isolate moisture, oxygen, and light, protecting light- and heat-sensitive active ingredients such as NMN and Coenzyme Q10, preventing their degradation and inactivation during storage; ② Targeted release into the intestine: Enteric coating resists the erosion of gastric acid, ensuring that the tablet core passes through the stomach intact and dissolves and disintegrates only in the slightly alkaline environment of the intestine. This not only avoids the damage of gastric acid to the active ingredients but also achieves a high concentration release of the active ingredients in the intestine, which is beneficial for absorption and thus significantly improves bioavailability; ③ Masking unpleasant odors and facilitating administration: The coating layer can mask the unpleasant odors or bitterness that plant extracts may bring, improving patient compliance; ④ Convenient industrial production: The tablet process is mature and easy to automate and scale up for large-scale industrial production, ensuring controllable quality.

[0049] In summary, enteric coating technology ensures the stability of active ingredients and their targeted release into the intestine, significantly improving bioavailability. Example 2 The antioxidant and anti-aging nutritional composition of this embodiment consists of the following raw materials in the indicated mass fractions: 40% β-nicotinamide mononucleotide (NMN), 10% coenzyme Q10, 30% plant extracts, 0.75% B vitamins, with the remainder being process additives. The process additives consist of microcrystalline cellulose (MCC), sodium carboxymethyl starch (CMS-Na), low-substituted hydroxypropyl cellulose (L-HPC), crospovidone (RPM), and magnesium stearate in a mass ratio of 10:5:3:2. The B vitamins are composed of the following ingredients in the indicated mass fractions: 38% vitamin B2 (riboflavin), 10% vitamin B9 (folic acid), 0.15% vitamin B12 (cobalamin), and the balance being vitamin B6 (pyridoxine).

[0050] The plant extract is composed of the following raw materials in the indicated mass fractions: 14% Polygonum cuspidatum extract, 21% Curcuma longa extract, 28% Jerusalem artichoke and chicory extract, 14% Lycium barbarum extract, and the remainder is jujube extract. The extraction method of the Polygonum cuspidatum extract is as follows: using 95% ethanol as solvent, reflux extraction is performed once at 80℃ for 2 hours. The extracts are combined, concentrated and dried to obtain the extract, wherein the resveratrol content is not less than 20%. The extraction method of the turmeric extract is as follows: using 95% ethanol as solvent, reflux extraction is performed once at 80°C for 2 hours each time. The extracts are combined, concentrated and dried to obtain the turmeric extract, wherein the curcumin content is not less than 80%. The extraction method of the said chicory and endive extract is: mixing the raw material with water at a ratio of 1:20, extracting at 85℃ with the aid of ultrasonic waves (power 300W) for 40 minutes, and obtaining the extract after concentration, alcohol precipitation and drying, wherein the inulin content is not less than 80%; Chicory / endive → cleaning → slicing or crushing → drying → pulverizing.

[0051] Hot water extraction (core step): Method: mixing the raw material with water in proportion, and extracting at a specific temperature and pH value.

[0052] Key parameters: Temperature: usually controlled at 70-85℃, too high temperature may lead to inulin hydrolysis into monosaccharides, and too low temperature may result in insufficient extraction rate.

[0053] pH value: usually in slightly acidic to neutral (pH 7.0) conditions to prevent inulin hydrolysis under acidic conditions.

[0054] Time: 4 times of extraction, each for about 60 minutes.

[0055] The extraction method of the said wolfberry extract is: mixing the raw material with water at a ratio of 1:25, extracting at 95℃ with the aid of ultrasonic waves (power 500W) for 40 minutes, and obtaining the extract after concentration, alcohol precipitation and drying, wherein the lycium barbarum polysaccharide content is not less than 30%; The extraction method of the said jujube extract is: mixing the raw material with water at a ratio of 1:25, extracting at 95℃ with the aid of ultrasonic waves (power 300-500W) for 40 minutes, and obtaining the extract after concentration, alcohol precipitation and drying, wherein the jujube polysaccharide content is not less than 25%.

[0056] The main component of the giant knotweed extract is white phytol; the main component of the turmeric extract is curcumin; the main component of the chicory and endive extract is inulin polysaccharide; the main component of the wolfberry extract is lycium barbarum polysaccharide, and the main component of the jujube extract is jujube polysaccharide.

[0057] The present embodiment also provides a tablet containing the above-mentioned antioxidant and anti-aging nutritional composition, which is made from the following raw materials by weight: polishing agent (magnesium stearate) 0.1 part, coating tablet 1 part; The said coating tablet comprises the following raw materials by weight: enteric coating material 10 parts, tablet core made of the said antioxidant and anti-aging nutritional composition 100 parts; The said enteric coating material is composed of hydroxypropyl methylcellulose phthalate (HPMCP) and polyethylene glycol 6000 (PEG6000) at a mass ratio of 4:1.

[0058] The embodiment also provides a method for preparing the tablet, and the method is as follows: S1, put β-nicotinamide mononucleotide, coenzyme Q10, plant extracts and B vitamins into a pulverizer to control temperature and refine particles, after pulverization, stand for 20 min, pass through a 200-mesh sieve, and obtain material a; Before use, the β-nicotinamide mononucleotide and coenzyme Q10 in the embodiment are respectively analyzed by HPLC, and the purity is greater than or equal to 99%, which is qualified; and the β-nicotinamide mononucleotide and coenzyme Q10 are dried under reduced pressure at 35°C until the moisture content is less than or equal to 3% before use. The rhizoma polgoni extract, curcuma longa extract, jerusalem artichoke and chichorium intybus extract, lycium barbarum extract and zizyphus jujuba extract in the plant extracts are weighed according to the proportion (without mixing), and can be dried under reduced pressure at 35°C until the moisture content is less than or equal to 3% before use. S2, put sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose and magnesium stearate into a powdering machine to control temperature and refine particles, after pulverization, stand for 100 min, pass through a 200-mesh sieve, and obtain material b; The low-substituted hydroxypropyl cellulose (L-HPC) is commercially available, and is purchased from Jinzhou Hengxiang Chemical Technology Co., Ltd. S3, put the material a obtained in S1 and the material b obtained in S2 into a V-type mixer, mix, then add microcrystalline cellulose and crosslinked povidone, mix for 40 min, stand for 10 min, and obtain the nutritional composition for antioxidation and anti-aging, denoted as material c; S4, tablet the material c obtained in S3 to obtain a tablet core of the nutritional composition for antioxidation and anti-aging; The tabletting method is as follows: use a preset mold, adjust the parameters of a tablet machine to 60 kN (6 tons), continuously tablet, collect untablet powder and tablets after tabletting, and count the number n1 of tablets. Control Q: 500g / n1=0.45~0.55; S5, add enteric coating materials to deionized water while stirring, stir for 60 min until completely dissolved, stand for 30 min to remove bubbles, and obtain a uniform and translucent enteric coating liquid; The mass ratio of the enteric coating material to deionized water is 1:8; S6, the tablet core obtained by tabletting is placed into a fluidized bed coating machine, the equipment is started, and after the fluidized state and temperature are stable, the enteric coating liquid obtained in S5 is sprayed onto the tablet core prepared from the antioxidant and anti-aging nutritional composition obtained in S4 to obtain a coated tablet; the spraying parameters are as follows: inlet air temperature is 38°C, material temperature is 32°C, atomization pressure is 1.2 Bar, and liquid spraying rate is 15 mL / min; the material temperature and the fluidized state are closely monitored during the coating process to ensure that the coating on the surface of the tablet core is uniform and complete; the coating is stopped when the weight of the tablet core is increased by 10% (calculated as dry solid) (i.e., the coated tablet comprises the following raw materials by weight: 10 parts of enteric coating material and 100 parts of tablet core); S7, after the coating is completed, the coated tablet obtained in S6 is continuously dried and solidified at a temperature of 35°C for 20 min to fully solidify the coating film, and then is transferred into a polishing pot, polishing agent is added, and low-speed rolling polishing is performed at a rotation speed of 15 rpm for 5 min to make the surface smooth, thereby obtaining the tablet of the antioxidant and anti-aging nutritional composition.

[0059] S8, quality inspection: random sampling, detection according to the relevant provisions of Chinese Pharmacopoeia. Key inspection: coating weight gain, tablet appearance (should be smooth surface, uniform color, no defects), and disintegration time limit: the disintegration instrument is used to test in a phosphate buffer solution with pH = 7.2 ± 0.2, and the tablet should be completely disintegrated within 4-6 h, and there is no hard core after disintegration.

[0060] Example 3 This example is an animal experiment.

[0061] The material c (i.e., the antioxidant and anti-aging nutritional composition) obtained in step S3 in Example 1 is weighed, dissolved with double distilled water, and prepared into a uniform suspension. The experimental group mice are gavaged at a dose of 500 mg / kg (500 mg of the antioxidant and anti-aging nutritional composition per kilogram of mouse body weight). The mice are 8-12 weeks old and 2-3 months old.

[0062] The young control group (3 months old) and the old control group (24 months old) mice are gavaged with the same volume of double distilled water at the same time point every day as a blank control.

[0063] There are 6 mice in each of the 3-month-old (young group), 24-month-old (old group), and experimental group (500 mg / kg) in this example, and the test data are averaged. The mice are all balb / c female mice, and the feeding environment is a clean room (10,000 level) with 15-30 lx light and 12 h / 12 h light and dark alternating time.

[0064] Gavage operation was performed at 7-8 am every day, and after 1 month, the mice were sacrificed by cervical dislocation, and the liver, brain and kidney tissues of the mice were collected for related experiments.

[0065] The oxidation levels of different tissues were determined by protein carbonyl content determination (DNPH method), glutathione peroxidase (GPx) activity determination (NADPH method), catalase (CAT) activity determination (H2O2 method) and SOD total enzyme activity determination (pyrogallol method) (126.11 g / mol).

[0066] Experimental results: The experimental results show that in the kidney tissue, the 24-month-old group (old age group) compared with the 3-month-old group (young group), the protein carbonylation level increased by 42.62%, and after feeding the nutritional composition of the application, the kidney protein carbonylation level of the experimental group mice decreased by about 20.13% compared with the old age group; in the brain tissue, the old age group compared with the young group, the protein carbonylation level increased by 39.63%, and the brain tissue protein carbonylation level of the experimental group decreased by about 31.58% compared with the old age group.

[0067] Table 1 Protein carbonyl content of tissues Note: The number of samples n=6, p≤0.05.

[0068] The results of total SOD content determination of tissues are shown in Table 2, which shows that in the brain tissue, the old age group compared with the young group, the total SOD level of the tissue decreased by 22.52%, and feeding the nutritional composition of the application increased by 19.90%; in the kidney tissue, the old age group compared with the young group, the total SOD level of the tissue decreased by 21.41%, and feeding the nutritional composition of the application increased by 7.79%; in the brain tissue, the old age group compared with the young group, the total SOD level of the tissue decreased by 29.16%, and the total SOD level of the tissue of the experimental group feeding the antioxidant and anti-aging nutritional composition increased by 8.85% compared with the old age group.

[0069] Table 2 Total SOD content of tissues Note: The number of samples n=6, p≤0.05.

[0070] The total SOD enzyme activity level determination results of the tissues are shown in Table 3. The results show that in the brain tissue, the total SOD enzyme activity of the old group is reduced by 29.36% compared with the young group, and is increased by 26.98% after feeding the nutritional composition of the application; in the kidney tissue, the total SOD level of the old group is reduced by 27.76% compared with the young group, and is increased by 9.25% after feeding the nutritional composition of the application; in the brain tissue, the total SOD level of the old group is reduced by 21.47% compared with the young group, and the total SOD level of the experimental group fed with the antioxidant and anti-aging nutritional composition of the application is increased by 7.89% compared with the old group.

[0071] Table 3 Analysis of total SOD enzyme activity level in tissues Note: The number of samples n = 6, p ≤ 0.05.

[0072] In summary, the animal experiment data of the embodiment fully show that the antioxidant and anti-aging nutritional composition of the application can significantly reverse the oxidative damage state of the aging organism (24-month-old mice) in multiple organs (liver, kidney, brain), which is specifically manifested in effectively reducing the protein carbonylation level related to aging and increasing the activity and content of important endogenous antioxidant enzymes (such as SOD).

[0073] These positive effects observed in model animals provide a solid scientific basis for the application of the composition to humans. Aging in humans is also accompanied by an increase in oxidative stress and a decline in the antioxidant enzyme system, which is closely related to the occurrence and development of various age-related diseases. Therefore, the nutritional composition can be expected to have similar health benefits of delaying aging, improving body function, and reducing the risk of age-related diseases in humans through its systematic antioxidant and cell energy activation effects.

[0074] Through in vivo and in vitro experiments, the antioxidant and anti-aging nutritional composition of the application has outstanding performance in delaying cell aging, reducing ROS accumulation, and enhancing the antioxidant defense capacity of the body.

[0075] The above is only a preferred embodiment of the application, and does not limit the application in any way. Any simple modification, change, and equivalent change made according to the technical essence of the application to the above embodiments are still within the protection scope of the technical solution of the application.

Claims

1. An antioxidant, anti-aging nutritional composition, characterized in that, The raw materials include β-nicotinamide mononucleotide, coenzyme Q10, plant extract, B vitamins and process accessories; The process accessories are composed of microcrystalline cellulose, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, cross-linked polyvinylpyrrolidone and magnesium stearate.

2. The nutritional composition according to claim 1, characterized in that, The raw materials are composed of β-nicotinamide mononucleotide 40%, coenzyme Q10 10%, plant extract 30%, B vitamins 0.75% and the rest of process accessories.

3. The nutritional composition according to claim 1, characterized in that, The plant extract is composed of rhizoma polygoni multiflori extract, curcuma longa extract, extract of jerusalem artichoke and chichorium intybus, lycium barbarum extract and jujube extract; The rhizoma polygoni multiflori extract and curcuma longa extract are extracted by ethanol solvent extraction method, and the extract of jerusalem artichoke and chichorium intybus, lycium barbarum extract and jujube extract are extracted by ultrasonic-assisted hot water extraction method.

4. The nutritional composition according to claim 3, characterized in that, The plant extract is composed of rhizoma polygoni multiflori extract 14%, curcuma longa extract 21%, extract of jerusalem artichoke and chichorium intybus 28%, lycium barbarum extract 14% and the rest of jujube extract.

5. The nutritional composition of claim 1, wherein the composition is antioxidant and anti-aging. The B vitamins are composed of vitamin B2, vitamin B9, vitamin B12 and vitamin B6.

6. The nutritional composition according to claim 5, characterized in that, The B vitamins are composed of vitamin B2 38%, vitamin B9 10%, vitamin B12 0.15% and the rest of vitamin B6.

7. The nutritional composition of claim 1, wherein the composition is formulated to be antioxidant and anti-aging. The process accessories are composed of microcrystalline cellulose, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, cross-linked polyvinylpyrrolidone and magnesium stearate with a mass ratio of 10:5:3:

2.

8. Tablets containing the antioxidant, anti-aging nutritional composition according to any one of claims 1 to 7, characterized in that, The tablet is made of the following raw materials: polishing agent 0.1-0.2 parts, coating tablet 1 part; The coating tablet includes the following raw materials: enteric coating material 8-10 parts, tablet core made of the antioxidant and anti-aging nutritional composition 100 parts.

9. The tablet according to claim 8, characterized in that, The polishing agent is magnesium stearate; The enteric coating material is composed of hypromellose phthalate and polyethylene glycol 6000 with a mass ratio of 4:

1.

10. A process for the preparation of a tablet according to claim 9, characterized in that, The method is: S1, powdering β-nicotinamide mononucleotide, coenzyme Q10, plant extract and B vitamins, and then standing for 20 min, and then passing through a 200-mesh sieve to obtain material a; S2, powdering sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose and magnesium stearate, and then standing for 100 min, and then passing through a 200-mesh sieve to obtain material b; S3, mixing material a obtained in S1 and material b obtained in S2, and then adding microcrystalline cellulose and cross-linked polyvinylpyrrolidone, and then mixing for 30-40 min, and then standing for 10 min to obtain the antioxidant and anti-aging nutritional composition, which is recorded as material c; S4, tabletting material c obtained in S3 to obtain the tablet core made of the antioxidant and anti-aging nutritional composition; S5, adding enteric coating material into deionized water while stirring, and then stirring for 45-60 min, and then standing for 30 min to remove bubbles to obtain enteric coating liquid; The mass ratio of the enteric coating material and deionized water is 1:

8. S6, the enteric coating liquid obtained in S5 is sprayed onto the tablet core prepared from the anti-oxidation and anti-aging nutritional composition obtained in S4 to obtain a coated tablet; the spraying parameters are as follows: the inlet air temperature is 38-42 DEG C, the material temperature is 32-35 DEG C, the atomization pressure is 0.8-1.2 Bar, and the liquid spraying rate is 10-15 mL / min; S7, the coated tablet obtained in S6 is further dried and solidified for 15-20 min at a temperature of 32-35 DEG C, then a polishing agent is added, and the polishing is performed at a rotating speed of 10-15 rpm for 5-10 min to obtain a tablet.

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