Vertical section-based naked carp otolith embedding method and otolith grinding sheet preparation method and application

By using an epoxy resin and curing agent encapsulation method and a sulfuric acid paper carrier, the problems of complex operation and high cost in the preparation of otolith grinding discs for naked carp have been solved. This method enables efficient and low-cost batch preparation of otolith grinding discs with clear wheel pattern structure, which is suitable for ecological research on naked carp.

CN121141307APending Publication Date: 2025-12-16HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202511399184.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-28
Publication Date
2025-12-16

AI Technical Summary

Technical Problem

Existing techniques for preparing otolith grinding discs from naked carp involve complex longitudinal sectioning, high costs, and low efficiency. Furthermore, conventional methods cannot meet the requirements for the firmness of otolith fixation, resulting in unclear wheel pattern structures in the otolith grinding discs.

Method used

An embedding agent made by mixing epoxy resin and curing agent in a specific ratio is used. Sulfate paper is used as a carrier. The otoliths of naked carp are embedded by longitudinal slicing and cured at room temperature. The process of polishing with wet sandpaper and nail polish is combined to ensure the adhesion and hardness of the otoliths, avoid delamination and breakage, and achieve mass production.

Benefits of technology

The process has been simplified, the cost has been reduced, and the preparation efficiency of otolith grinding discs and the clarity of the wheel pattern structure have been improved, making them suitable for ecological research on naked carp.

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Abstract

The invention belongs to the technical field of fish ecology, and particularly relates to a nude carp otolith embedding method based on a longitudinal section and a preparation method and application of an otolith grinding sheet. The epoxy resin and the curing agent are mixed according to a certain mass ratio and then placed on the vegetable parchment to embed the longitudinal section of the naked carp otolith, the vegetable parchment is resistant to high temperature and high in strength and is not prone to deformation caused by high temperature, and the cured embedding piece is easily separated from the vegetable parchment; the embedding agent is low in shrinkage rate after being cured, the otolith is not prone to fracture, the hardness requirement of subsequent grinding can be met, adhesion is high, degumming is avoided in the subsequent grinding process, and fragmentation of the naked carp otolith is effectively avoided. According to the method, the embedding piece polished for the first time is immediately immersed in water, oxidation of the polished surface of the otolith can be prevented, subsequent polishing is continued, and the otolith grinding piece with clear wheel lines is obtained. The method is simple in operation process, does not need to use a high-cost and low-efficiency embedding mold, reduces the preparation cost, and improves the preparation efficiency.
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