Phyllanthus emblica extract capable of preserving moisture, resisting oxidation, resisting inflammation, resisting irritation, promoting collagen production, inhibiting photochemical products or promoting wound healing

Abstract

The invention relates to an application of a phyllanthus emblica extract. The phyllanthus emblica extract is used for preparing a composition for preserving moisture, resisting oxidation, resisting inflammation, resisting irritation, promoting collagen generation, inhibiting photochemical products or promoting wound healing.

Description

TECHNICAL FIELD

[0001] The present application relates to a plant extract, in particular to a Phyllanthus emblica extract, and the composition prepared therefrom can be used as a raw material for medical products, cosmetics, care products, fragrances, or human cleaning products. BACKGROUND

[0002] Phyllanthus emblica, the fruit of which can be eaten raw, used as a side dish, or made into preserves for food purposes, is commonly used as a raw material for food processing. However, the existing processing method cannot effectively obtain the active ingredients contained in Phyllanthus emblica. Although Phyllanthus emblica has many nutrients, it is currently rarely used in other fields of products, which is a pity. In order to properly utilize the effective components contained in Phyllanthus emblica and make it play a greater commercial value, it is necessary to put it into other fields. SUMMARY

[0003] The problem to be solved by the present application is to extract the effective components contained in Phyllanthus emblica and put the extract prepared from Phyllanthus emblica into cosmetic products, care products, fragrances, or human cleaning products.

[0004] To solve the above problems, the present application provides a use of a Phyllanthus emblica extract for preparing a composition for moisturizing, antioxidant, anti-inflammatory, anti-irritation, collagen production promotion, actinic product inhibition, or wound healing promotion.

[0005] More preferably, the preparation method of the Phyllanthus emblica extract sequentially comprises: a crushing step, selecting a weight ratio of 1:2 to 10 of Phyllanthus emblica fruit and liquid nitrogen, first freezing the Phyllanthus emblica fruit with the liquid nitrogen for 10 to 60 seconds, then thawing at room temperature for 10 to 30 seconds, and repeating the cycle 2 to 6 times to make the cell wall of the Phyllanthus emblica fruit rupture and obtain Phyllanthus emblica fruit rupture; a soaking step, selecting a weight ratio of 1:1 to 10 of the Phyllanthus emblica fruit rupture and an extraction solvent, and soaking the Phyllanthus emblica fruit rupture in the extraction solvent for 8 to 24 hours to obtain a mixed solution; and an ultrasonic oscillation step, continuously and rapidly oscillating the mixed solution with an ultrasonic energy of 300W to 1200W to extract for 5 to 15 minutes to obtain a crude extract.

[0006] More preferably, it further comprises a reduced pressure concentration step, wherein the crude extract is subjected to reduced pressure concentration at a temperature of 50 to 65°C to remove the excess extraction solvent contained therein and obtain a concentrated solution.

[0007] More preferably, the method further comprises a washing step, wherein the Phyllanthus emblica fruits are washed before the crushing step.

[0008] More preferably, the method further comprises a drying step, wherein the Phyllanthus emblica fruits are dried before the crushing step to obtain dried Phyllanthus emblica fruits.

[0009] More preferably, the method further comprises a filtering step, wherein the crude extract is filtered to remove solid-phase components contained in the crude extract and obtain a liquid-phase extract.

[0010] More preferably, the method further comprises a filtering step, wherein the crude extract is filtered to remove liquid-phase components contained in the crude extract and obtain a solid-phase extract.

[0011] More preferably, in the ultrasonic oscillation step, the oscillation extraction is performed for 5 to 15 minutes for one cycle, and the cycle is repeated at least once.

[0012] More preferably, the extraction solvent is water or an ethanol solution having a concentration of 75% to 95%.

[0013] More preferably, the Phyllanthus emblica extract has a concentration of 100 to 500 pg / ml.

[0014] The effect of the present application over the prior art is that the present application can deeply extract active ingredients contained in Phyllanthus emblica fruits and use the obtained Phyllanthus emblica extract as a raw material in cosmetic products, care products, fragrances, or human body cleansing products, etc., so that the user can achieve the effects of moisturizing, antioxidant, anti-inflammatory, anti-irritation, collagen production promotion, actinic product inhibition, or wound healing promotion when applying the above products to human skin. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figures 1A to 1E is a series of flowcharts for illustrating the preparation process of the Phyllanthus emblica extract;

[0016] Figure 2A is a bar graph for illustrating the effect of the Phyllanthus emblica extract on the survival rate of HaCaT cells;

[0017] Figure 2B is a bar graph for illustrating the effect of the Phyllanthus emblica extract on the survival rate of 3T3L1 cells;

[0018] Figure 3 is a bar graph for illustrating the effect of the Phyllanthus emblica extract on the ABTS free radical scavenging rate;

[0019] Figure 4 is a photograph for illustrating the results of the superoxide anion (·O2 - ) after staining;

[0020] Figures 5A to 5D A series of histograms showing the inhibitory effect of Phyllanthus emblica extract on hyaluronidase;

[0021] Figure 6 A graph showing the effect of Phyllanthus emblica extract on the expression of AQP-3 protein;

[0022] Figure 7 A histogram showing the protective ability of Phyllanthus emblica extract on DNA;

[0023] Figure 8 A photograph showing the anti-irritation ability of Phyllanthus emblica extract;

[0024] Figure 9 A graph showing the ability of Phyllanthus emblica extract to inhibit inflammatory factors;

[0025] Figure 10 A photograph showing the ability of Phyllanthus emblica extract to inhibit UV photochemical products of cells;

[0026] Figure 11 A histogram showing the effect of Phyllanthus emblica extract on extracellular collagen content;

[0027] Figure 12 A line graph showing the ability of Phyllanthus emblica extract to promote wound healing of cells. DETAILED DESCRIPTION

[0028] In order to make the above and other objects, effects, and features of the present application more apparent, a preferred embodiment will be described in detail hereinafter with reference to the accompanying drawings:

[0029] The present application aims to provide a use of Phyllanthus emblica extract for preparing a composition for moisturizing, anti-oxidation, anti-inflammation, anti-irritation, promoting collagen production, inhibiting photochemical products, or promoting wound healing. In a preferred embodiment, in order to extract Phyllanthus emblica to obtain active ingredients contained therein, such as Figure 1AAs shown, the preparation method of the Phyllanthus emblica extract comprises: a crushing step (1) of selecting Phyllanthus emblica fruits and liquid nitrogen in a weight ratio of 1:2 to 10, first freezing the Phyllanthus emblica fruits with the liquid nitrogen for 10 to 60 seconds, then thawing at room temperature for 10 to 30 seconds, and repeating the cycle 2 to 6 times, so that the cell walls of the Phyllanthus emblica fruits are broken, and Phyllanthus emblica fruit broken material is obtained; a soaking step (2) of selecting the Phyllanthus emblica fruit broken material and an extraction solvent in a weight ratio of 1:1 to 10, and soaking the Phyllanthus emblica fruit broken material in the extraction solvent for 8 to 24 hours to obtain a mixed solution; and an ultrasonic oscillation step (3) of continuously and rapidly oscillating the mixed solution with ultrasonic energy of a total energy of 300 W to 1200 W for 5 to 15 minutes to obtain a crude extract. In another preferred embodiment, the concentration of the Phyllanthus emblica extract is 100 to 500 μg / ml, but is not limited thereto.

[0030] More preferably, in order to obtain a Phyllanthus emblica extract with a high concentration of active ingredients, as shown in Figure 1B which further comprises: a reduced pressure concentration step (4) of reducing the pressure and concentrating the crude extract at a temperature of 50 to 65°C to remove the excess extraction solvent contained therein, and obtaining a concentrated solution. In a preferred embodiment, in order to avoid impurities or foreign matter from being mixed into the extract, thereby causing the active ingredients contained in the extract to deteriorate, as shown in Figure 1C which further comprises: a washing step (5) of washing the Phyllanthus emblica fruits before the crushing step (1). In another preferred embodiment, in order to improve the extraction rate of the extract, as shown in Figure 1D which further comprises: a drying step (6) of drying the Phyllanthus emblica fruits before the crushing step (1) to obtain dried Phyllanthus emblica fruits. In yet another preferred embodiment, in order to obtain the liquid phase components in the crude extract, as shown in Figure 1E which further comprises: a filtration step (7) of filtering the crude extract to remove the solid phase components contained in the crude extract, and obtaining a liquid phase extract. In yet another preferred embodiment, in order to obtain the solid phase components in the crude extract, as shown in Figure 1E which further comprises: a filtration step (7) of filtering the crude extract to remove the liquid phase components contained in the crude extract, and obtaining a solid phase extract. In yet another preferred embodiment, in order to preserve the Phyllanthus emblica extract and maintain the activity of the active ingredients contained in the Phyllanthus emblica extract for a long time, it further comprises: a freeze-drying step of freeze-drying the concentrated solution to obtain a freeze-dried product. In yet another preferred embodiment, in order to improve the types and concentrations of the active ingredients contained in the Phyllanthus emblica extract, it further comprises: a liquid nitrogen freeze-solidification step of freeze-solidifying the Phyllanthus emblica fruits with liquid nitrogen before the soaking step (2) to destroy the fibers in the Phyllanthus emblica fruits.

[0031] More preferably, in order to perform deep extraction of Phyllanthus emblica to obtain an extract with high concentration and rich in active ingredients, the extraction time in the ultrasonic oscillation step (3) is 5 to 15 minutes for one cycle, and the cycle is continued for at least one time, preferably 3 to 5 times. In a preferred embodiment, the extraction solvent is water or an ethanol solution with a concentration of 75% to 95%, but is not limited thereto.

[0032] The Phyllanthus emblica extract of the present application is obtained by extracting fresh Phyllanthus emblica fruits and dried Phyllanthus emblica fruits with water and ethanol, respectively. Four types of Phyllanthus emblica extracts can be obtained, as shown in Table 1 below.

[0033] Table 1. Classification of Phyllanthus emblica extracts

[0034]

[0035] Preferably, the preparation method of the Phyllanthus emblica fruit water extract (PEFW) comprises: selecting 200 g of Phyllanthus emblica fruits and 1800 g of pure water, immersing the Phyllanthus emblica fruits in the pure water for 0.5 to 1 hour to obtain a mixed solution A; oscillating the mixed solution A at an ultrasonic energy of 300 W for 1.5 hours to obtain a crude extract A; performing vacuum concentration of the crude extract A at a temperature of 50°C to remove excess solvent contained therein and obtain a concentrated solution A; and freeze-drying the concentrated solution A to obtain a freeze-dried product A, wherein the obtained freeze-dried product A is the Phyllanthus emblica fruit water extract (PEFW). The obtained Phyllanthus emblica fruit water extract (PEFW) is weighed and the extraction rate is calculated, wherein: extraction rate = (extract weight / extract raw material weight) * 100%, and the extraction rate of the Phyllanthus emblica fruit water extract (PEFW) is finally obtained as 6.03%.

[0036] Preferably, the preparation method of the ethanol extract of Phyllanthus emblica fruit (PEFE) comprises: selecting 200 g of Phyllanthus emblica fruit and 200 g of 95% ethanol, and soaking the Phyllanthus emblica fruit in the 95% ethanol for 72 hours to obtain a mixed solution B; subjecting the mixed solution B to ultrasonic extraction at 300-600 W for 1.5 hours to obtain a crude extract B; performing vacuum concentration on the crude extract B at a temperature of 45-50°C to remove excess solvent contained therein and obtain a concentrated solution B; and performing freeze-drying on the concentrated solution B to obtain a freeze-dried product B, wherein the obtained freeze-dried product B is the ethanol extract of Phyllanthus emblica fruit (PEFE). The obtained ethanol extract of Phyllanthus emblica fruit (PEFE) is weighed and the extraction rate is calculated, wherein: extraction rate = (extract weight / extract raw material weight)*100%, and finally the extraction rate of the ethanol extract of Phyllanthus emblica fruit (PEFE) is 5.22%.

[0037] Preferably, the preparation method of the water extract of Phyllanthus emblica dried fruit (PEDW) comprises: selecting 36.11 g of Phyllanthus emblica dried fruit and 324.99 g of pure water, and soaking the Phyllanthus emblica dried fruit in the pure water for 0.5-1 hour to obtain a mixed solution C; subjecting the mixed solution C to ultrasonic extraction at 300 W for 1.5 hours to obtain a crude extract C; performing vacuum concentration on the crude extract C at a temperature of 50°C to remove excess solvent contained therein and obtain a concentrated solution C; and performing freeze-drying on the concentrated solution C to obtain a freeze-dried product C, wherein the obtained freeze-dried product C is the water extract of Phyllanthus emblica dried fruit (PEDW). The obtained water extract of Phyllanthus emblica dried fruit (PEDW) is weighed and the extraction rate is calculated, wherein: extraction rate = (extract weight / extract raw material weight)*100%, and finally the extraction rate of the water extract of Phyllanthus emblica dried fruit (PEDW) is 13.10%.

[0038] Preferably, the preparation method of the ethanol extract of dried Phyllanthus emblica fruit (PEDE) comprises: selecting 35.46 g of dried Phyllanthus emblica fruit and 35.46 g of 95% ethanol, and soaking the dried Phyllanthus emblica fruit in the 95% ethanol for 72 hours to obtain a mixed solution D; subjecting the mixed solution D to ultrasonic extraction at an ultrasonic energy of 300 to 600 W for 1.5 hours to obtain a crude extract D; subjecting the crude extract D to vacuum concentration at a temperature of 45 to 50°C to remove excess solvent contained therein and obtain a concentrated solution D; and subjecting the concentrated solution D to freeze-drying to obtain a freeze-dried product D, wherein the obtained freeze-dried product D is the ethanol extract of dried Phyllanthus emblica fruit (PEDE). The obtained ethanol extract of dried Phyllanthus emblica fruit (PEDE) is weighed and the extraction rate is calculated, wherein the extraction rate = (weight of the extract / weight of the raw material for extraction) * 100%, and finally the extraction rate of the ethanol extract of dried Phyllanthus emblica fruit (PEDE) is 24.00%.

[0039] The extraction conditions and extraction rates of the water extract of Phyllanthus emblica fruit (PEFW), the ethanol extract of Phyllanthus emblica fruit (PEFE), the water extract of dried Phyllanthus emblica fruit (PEDW), and the ethanol extract of dried Phyllanthus emblica fruit (PEDE) are summarized in Table 2 below.

[0040] Table 2

[0041] Extract Extraction solvent Extraction feedstock weight (g) Extract weight (g) Extraction yield (%) PEFW Pure water 200 12.05 6.03％ PEFE 95% ethanol 200 10.43 5.22％ PEDW Pure water 36.11 4.73 13.10％ PEDE 95% ethanol 35.46 8.51 24.00％

[0042] To demonstrate that the Phyllanthus emblica extracts of the present application can achieve the effects of moisturizing, antioxidant, anti-inflammatory, anti-irritation, promoting collagen production, inhibiting photochemical products, or promoting wound healing, the following experimental results are provided to illustrate.

[0043] Experiment 1: Test of the effects of Phyllanthus emblica extracts on cell viability of human skin keratinocyte HaCaT cells and mouse embryonic fibroblasts (3T3L1).

[0044] In this experiment, first, PEFW, PEFE, PEDW, and PEDE at concentrations of 100 μg / ml to 1000 μg / ml were selected, and each extract at each concentration was allowed to act on human skin keratinocyte HaCaT cells for 24 hours, as shown in Figure 2A It was found that when the concentration of each extract was 100 μg / ml to 500 μg / ml, the cell viability of human skin keratinocyte HaCaT cells was higher than 80%.

[0045] Then, PEFW at concentrations of 25 μg / ml to 1000 μg / ml was selected, and allowed to act on mouse embryonic fibroblasts (3T3L1) for 48 hours, as shown in Figure 2BAs shown in Table 1, it can be found that the cell viability of mouse embryonic fibroblast (3T3L1) is higher than 80% when the concentration of the extract is 25 μg / ml to 200 μg / ml, and the cell viability of mouse embryonic fibroblast (3T3L1) is lower than 80% when the concentration of the extract is 500 μg / ml to 1000 μg / ml, indicating that PEFW has no toxicity to mouse embryonic fibroblast (3T3L1) after 48 hours of action at a concentration of 25 μg / ml to 200 μg / ml.

[0046] Experiment 2: Antioxidant capacity test of the water extract and the ethanol extract of Phyllanthus emblica.

[0047] For the in vitro antioxidant test of Phyllanthus emblica, ABTS·+ free radicals were used to determine the antioxidant capacity, and the results are shown in Table 2. Figure 3 As shown in Table 2, PEFW, PEFE, PEDW and PEDE have good ABTS·+ free radical scavenging effect at low concentrations, and the highest inhibition effect can be achieved when the concentration of the extract is 50 μg / ml, which is similar to the inhibition effect of the standard AA. In addition, when the concentration of PEFW, PEFE, PEDW and PEDE is 22.0, 17.1, 41.7 and 16.4 μg / ml, respectively, each extract has the ability to scavenge 50% of ABTS·+ free radicals. The results show that the antioxidant capacity of the ethanol extract of Phyllanthus emblica is better than that of the water extract, and among all the extracts, PEDE has the best effect on scavenging ABTS·+ free radicals.

[0048] Experiment 3: Determination test of the content of superoxide anion (·O2 - ) in cells treated with the extract of Phyllanthus emblica.

[0049] Reactive oxygen species are biochemical reactions related to the oxidation and reduction of oxygen molecules in human cells, which are normal metabolic functions. When the concentration of superoxide anion (·O2 - ) in the human body is too high, it can cause damage to cell components such as proteins, lipids and nucleic acids, and change the oxidation and reduction balance. In this experiment, PEFW was used to treat HaCaT cells for 24 hours, then H2O2 was used to induce HaCaT cells to produce superoxide anion (·O2 - ), and MitoSOX staining was used to determine the content of superoxide anion (·O2 - ) in HaCaT cells. The results are shown in Table 3. Figure 4 As shown in Table 3, after the human skin keratinocyte strain HaCaT was induced by H2O2, a large amount of superoxide anion (·O2 - ) was produced, and after MitoSOX staining, superoxide anion (·O2 - ) produced strong red fluorescence. Figure 4The position indicated by the white arrow is the position of red fluorescence emitted by superoxide anion (·O2 - ).

[0050] Experiment 4: Inhibition of hyaluronidase activity by extracts of Phyllanthus emblica.

[0051] Hyaluronic acid is a skin moisturizing factor that exists in human connective tissue and the dermis layer of the skin, and has various physiological effects, such as promoting wound healing, promoting skin repair, or improving vascular permeability. In addition, hyaluronic acid is currently one of the commonly used moisturizing ingredients in cosmetics due to its excellent water retention capacity. In this experiment, hyaluronic acid was added to the film, and the activity of hyaluronidase (HAase) was inhibited to prevent the film from being dyed blue by the reagent, thereby determining the activity of the hyaluronidase inhibitor. As shown in Figures 5A to 5D Figure 2, the inhibitory effect of PEFW, PEDW, PEFE, and PEDE on hyaluronidase was 52.0%, 31.9%, 43.0%, and 41.6%, respectively, indicating that PEFW, PEDW, PEFE, and PEDE all have the ability to inhibit hyaluronidase, and PEFW has the best inhibitory effect among all the extracts.

[0052] Experiment 5: Moisturizing ability of extracts of Phyllanthus emblica.

[0053] Collagen and elastin are the main support structures of the dermis layer of the skin, and aquaporin (AQP-3) is a channel protein in the skin that is used to transfer water and glycerol, and is closely related to the skin barrier function. When the skin is damaged by UV rays, the aquaporin protein is decomposed, causing skin aging, wrinkles, dryness, and desquamation. As shown in Figure 6 Figure 3, after UVB irradiation of 3T3L1 cells, the expression of AQP-3 protein in the cells decreased from 47.2% to 21.2%; after treatment with PEFW at concentrations of 50, 100, and 200 μg / ml, the expression of AQP-3 protein increased to 32.6%, 35.6%, and 36.2%, respectively. These results confirm that PEFW has the ability to protect and promote the expression of aquaporin.

[0054] Experiment 6: DNA protection ability of extracts of Phyllanthus emblica.

[0055] Normal DNA is in supercoiled-form (S-form), but after UVB irradiation and oxidative stress damage, its circular structure is formed into linear-form (L-form). Thus, the difference in the moving distance of nucleic acids with different molecular weights in the gel pore size can be used to determine the DNA protection ability. In this experiment, H2O2 (1 mM), FeSO4 (0.5 mM) induction, and UVB (20 mJ / cm 2 ) irradiation were used to damage pUC119 DNA and form DNA damage. As shown in Figure 7 , after H2O2, FeSO4, and UVB damage, the supercoiled DNA was only about 59.9% left, while after the addition of 100 μg / ml and 200 μg / ml PEFW, the supercoiled DNA still retained 97.4% and 82.1%, respectively, which was the best among all extracts in terms of DNA protection ability. In summary, 100 μg / ml and 200 μg / ml PEFW can protect DNA from UV light and oxidative stress damage.

[0056] Experiment 7: Evaluation of the anti-irritation ability of Emblica extract.

[0057] Calcium ions (Ca 2+ ) are channel signals that regulate keratin and cell function in cells. When there is too much calcium ion flow in and out of cells, it indicates damage or irritation to the skin barrier function, causing redness, itching, or inflammation. Phenoxyethanol is a common preservative used in cosmetics, with a regulatory addition of 1%. It can inhibit the growth of gram-negative bacteria, gram-positive bacteria, and Candida albicans. However, preservatives in cosmetics are also a common cause of sensitive skin. As shown in Figure 8 , after 1% phenoxyethanol induction for 5 minutes, a large amount of calcium ions flowed into the cells, causing the cells to emit green fluorescence. The addition of PEFW reduced the expression of green fluorescence, and the inhibitory effect was better as the concentration increased. The above results confirmed that PEFW can inhibit the expression of calcium ion channels induced by phenoxyethanol.

[0058] Experiment 8: Evaluation of the anti-inflammatory factor inhibition ability of Emblica extract.

[0059] The inflammatory factor TRPV1 is involved in various pain and inflammation pathways. When the TRPV1 channel is opened, it connects to downstream signals, leading to the production of irritation, pain, and inflammation. HaCaT cells were induced with 10 mM capsaicin, and PEFW was added for 24 hours. The expression of irritation factors was determined using flow cytometry. The results are shown in Figure 9As shown, after induction by capsaicin, TRPV1 and TAK-1 factors will be expressed in large quantities, thus it can be confirmed that PEFW can inhibit the expression of TRPV1 caused by capsaicin, and further inhibit the expression of downstream inflammatory factor TAK-1, so as to reduce the stimulation and the generation of pain, and achieve the effect of anti-inflammation.

[0060] Experiment 9: Inhibition test of the UVB photo-product content of the cells by the extract of Phyllanthus emblica.

[0061] When the cells are exposed to ultraviolet radiation for a long time, in addition to causing melanin production and photoaging, it may also cause genetic mutations in the cells. Among them: when the cells are exposed to UVB radiation, it will cause the generation of UVB photo-product cyclobutane pyrimidine dimer (CPD), which is an indicator of DNA damage caused by UVB. As shown, CPD was used for immunofluorescence staining to determine the DNA damage in the cells, wherein: the group after UVB irradiation emits a strong fluorescence signal of photo-product; and the group after PEFW treatment, the higher the concentration of the extract applied, the better the inhibition effect on photo-product, which can confirm that PEFW can repair DNA damage induced by UVB and reduce the generation of UVB photo-product. Figure 10

[0062] Experiment 10: Effect of the extract of Phyllanthus emblica on the content of extracellular collagen.

[0063] Collagen is the main component that supports the structure of the dermis layer of the skin. When collagen and extracellular matrix are destroyed, it will cause skin aging and wrinkles. In addition, when the skin is exposed to ultraviolet radiation for a long time, it is easy to cause collagen to degrade and deform. In order to enhance the structure and elasticity of the skin, it is necessary to inhibit the degradation of collagen. Among them, ascorbic acid (AA) has strong antioxidant ability and can promote the effect of collagen in the skin, so it is used as a control group in this experiment. As shown, when 25 μg / ml to 100 μg / ml of PEFW is applied to mouse embryonic fibroblasts (3T3L1), it can promote the generation of extracellular collagen, and the promotion effect is better than AA. Figure 11

[0064] Experiment 11: Wound healing test of the cells by the extract of Phyllanthus emblica.

[0065] ​​The present experiment added PEFW to serum-free culture medium and observed the movement of HaCaT cells at different time points using a microscope to evaluate the ability of PEFW to promote wound healing. Among them: compared with the control group, the group added PEFW had a growth trend towards the middle after 24 hours of action, and the effect of promoting wound healing was better with the increase of PEFW concentration. Specifically, as shown in Figure 12 The distance between cells decreased from 100% to 50% after 24 hours of action, and the distance between cells was reduced to less than 20% after 48 hours of action, almost completely healed, indicating that PEFW has excellent ability to promote wound healing.

[0066] In summary, the efficacy of the present application relative to the prior art is that the present application can extract active ingredients contained in the Phyllanthus emblica fruit, and apply the extracted Phyllanthus emblica extract as a raw material in products such as cosmetic products, care products, fragrances, or human cleaning products, so that the user can achieve the effects of moisturizing, antioxidant, anti-inflammatory, anti-irritation, collagen production promotion, actinic product inhibition, or wound healing promotion when applying the above products to human skin.

[0067] The above-mentioned is only a preferred embodiment of the present application, which cannot limit the scope of patent protection of the present application, that is, any simple equivalent changes and modifications made according to the scope of patent protection and the content of the present application fall within the scope of patent protection of the present application. In addition, any embodiment or patent protection scope of the present application does not necessarily achieve all the purposes, advantages or features disclosed in the present application. In addition, the abstract and title are only used to assist in patent file searching, and are not used to limit the scope of patent protection of the present application. In addition, the terms first, second, etc. mentioned in the specification are only used to represent the names of components, and are not used to limit the upper or lower limit of the number of components.

[0068]

Symbol Description

[0069] 1 Breakage step

[0070] 2 Soaking step

[0071] 3 Ultrasonic vibration step

[0072] 4 Reduced pressure concentration step

[0073] 5 Washing step

[0074] 6 Drying step

[0075] 7 Filtering step

Claims

1. Use of a Phyllanthus emblica extract, characterized in that, A composition for preparing a moisturizing, antioxidant, anti-inflammatory, anti-irritant, collagen production promoting, actinic product inhibiting, or wound healing promoting composition.

2. Use according to claim 1, characterized in that, The preparation method of the Phyllanthus emblica extract sequentially comprises: a crushing step, selecting a weight ratio of 1:2 to 10 of Phyllanthus emblica fruits and liquid nitrogen, first freezing the Phyllanthus emblica fruits with the liquid nitrogen for 10 to 60 seconds, then thawing at room temperature for 10 to 30 seconds, and repeating the cycle 2 to 6 times to break the cell walls of the Phyllanthus emblica fruits and obtain Phyllanthus emblica fruit rupture; a soaking step, selecting a weight ratio of 1:1 to 10 of the Phyllanthus emblica fruit rupture and an extraction solvent, and soaking the Phyllanthus emblica fruit rupture in the extraction solvent for 8 to 24 hours to obtain a mixed solution; and an ultrasonic oscillation step, continuously and rapidly oscillating the mixed solution with ultrasonic energy of a total energy of 300 W to 1200 W for 5 to 15 minutes to obtain a crude extract.

3. Use according to claim 2, characterized in that, Further comprising: a reduced pressure concentration step, reducing the pressure and concentrating the crude extract at a temperature of 50 to 65°C to remove excess extraction solvent contained therein, and obtaining a concentrated solution.

4. Use according to claim 2, characterized in that, Further comprising: a washing step, washing the Phyllanthus emblica fruits before the crushing step.

5. Use according to claim 2, characterized in that, Further comprising: a drying step, drying the Phyllanthus emblica fruits before the crushing step to obtain dried Phyllanthus emblica fruits.

6. Use according to claim 2, characterized in that, Further comprising: a filtering step, filtering the crude extract to remove solid phase components contained in the crude extract and obtain a liquid phase extract.

7. Use according to claim 2, characterized in that, Further comprising: a filtering step, filtering the crude extract to remove liquid phase components contained in the crude extract and obtain a solid phase extract.

8. Use according to claim 2, characterized in that, In the ultrasonic oscillation step, the oscillation extraction time is 5 to 15 minutes for one cycle, and the cycle is continued at least once.

9. Use according to claim 2, characterized in that, The extraction solvent is water or an ethanol solution with a concentration of 75% to 95%.

10. Use according to claim 1, characterized in that, The concentration of the Phyllanthus emblica extract is 100 to 500 μg / ml.

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