Composition capable of improving lung deficiency and nourishing lung and tonifying qi as well as preparation method and application of composition

Abstract

The invention discloses a composition for improving lung deficiency and having effects of nourishing lung and tonifying qi as well as a preparation method and application of the composition. The composition is prepared from the following components in parts by weight: 20 to 30 parts of folium eriobotryae extract, 5 to 15 parts of loquat flower extract, 25 to 35 parts of cordyceps militaris extract, 25 to 35 parts of fructus lycii extract, 5 to 12 parts of rhizoma polygonati extract, 3 to 12 parts of ginseng oligopeptide and 0.5 to 3 parts of gamma-aminobutyric acid. The invention also provides a preparation method of the composition and application of the composition prepared by the method. The composition is used for preparing an oral preparation which is used for improving lung deficiency and has the effects of nourishing lung and tonifying qi. The composition provided by the invention has the characteristics of multi-target synergism, safety, high efficiency, reasonable formula and the like, can achieve the effects of moistening lung, reducing phlegm, tonifying qi, nourishing yin, enhancing immunity and the like, and is suitable for improving the syndromes of deficiency of lung qi and deficiency of both qi and yin.

Description

TECHNICAL FIELD

[0001] The present application relates to a traditional Chinese medicine preparation composition with the effect of nourishing the lung and benefiting qi, and a preparation method and application thereof, in particular to a composition for improving the lung deficiency with the effect of nourishing the lung and benefiting qi, and a preparation method and application thereof. BACKGROUND

[0002] Pulmonary disease refers to diseases of the lung itself or pulmonary manifestations of systemic diseases. The respiratory system is composed of the respiratory tract (nose, pharynx, larynx, trachea and bronchus) and alveoli. The lung is the main organ of the respiratory system, and pulmonary disease belongs to respiratory disease. The lung is also closely related to the cardiovascular system. In addition to the respiratory function, the lung also has non-respiratory defense, immune and endocrine metabolic functions.

[0003] Studies have shown that the main factors affecting the current situation of lung disease and lung health problems include smoking, air pollution, respiratory tract infection, occupational hazards and tuberculosis. The above factors have led to an increasing number of people with lung qi deficiency in modern environment, and the clinical manifestations include shortness of breath, fatigue, cough and sputum, and decreased immunity.

[0004] Lung qi deficiency, also known as lung qi deficiency, refers to the weakening of the physiological function of the lung. According to the theory of traditional Chinese medicine, lung deficiency refers to the functional disorder caused by lung qi or lung yin deficiency, which can be divided into lung qi deficiency and lung yin deficiency. The main symptoms of lung qi deficiency are shortness of breath, susceptibility to cold, and lung yin deficiency is characterized by dry cough, dry throat and dry tongue. Chronic disease may affect the spleen and kidney. For this disease, the treatment plan of traditional Chinese medicine is generally based on tonifying lung qi and nourishing yin.

[0005] However, the drugs for lung deficiency on the market have certain defects, the most prominent of which include: the traditional Chinese medicine decoction has poor taste and is inconvenient to take; single component preparations such as cordycepin capsules have limited efficacy. The use of chemical synthetic drugs has a high risk of side effects. SUMMARY

[0006] The purpose of the present application is to provide a composition with the effect of nourishing the lung and benefiting qi composed of natural plants and active extracts, and a preparation method and application thereof, which can solve the existing problems and be suitable for improving lung qi deficiency and qi-yin deficiency syndrome.

[0007] In order to achieve the above purpose, the present application provides a composition for improving lung deficiency with the effect of nourishing the lung and benefiting qi, wherein the composition is composed of the following ingredients: loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, ginseng oligopeptide and gamma-aminobutyric acid.

[0008] The composition for improving lung deficiency and having the function of nourishing lung and benefiting qi, wherein the composition is composed of the following ingredients in parts by weight: 20-30 parts of loquat leaf extract, 5-15 parts of loquat flower extract, 25-35 parts of cordyceps militaris extract, 25-35 parts of medlar extract, 5-12 parts of polygonatum extract, 3-12 parts of ginseng oligopeptide and 0.5-3 parts of gamma-aminobutyric acid.

[0009] The composition for improving lung deficiency and having the function of nourishing lung and benefiting qi, wherein the composition is composed of the following ingredients in parts by weight: 25 parts of loquat leaf extract, 10 parts of loquat flower extract, 30 parts of cordyceps militaris extract, 28 parts of medlar extract, 8 parts of polygonatum extract, 6 parts of ginseng oligopeptide and 1.5 parts of gamma-aminobutyric acid.

[0010] The application further provides a preparation method of the composition, wherein the method comprises the following steps: step 1, preparing loquat leaf extract; step 2, preparing loquat flower extract; step 3, preparing cordyceps militaris extract; step 4, preparing medlar extract; step 5, preparing polygonatum extract; step 6, weighing the obtained loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, ginseng oligopeptide and gamma-aminobutyric acid according to the proportion respectively, then mixing the ingredients uniformly, drying the mixture to make the water content of the mixture less than or equal to 5% in mass percentage, crushing the mixture into fine powder, then passing through an 80-100 mesh sieve to obtain the composition, sterilizing the composition and then sealing the composition in bags for use.

[0011] The method for preparing the composition, wherein the step 1 comprises: step 1.1, collecting loquat leaves, washing, drying and crushing into powder, and passing through a 16-20 mesh sieve; step 1.2, adding distilled water to the obtained powder at a material-liquid ratio of 1:12-18 to perform extraction at a temperature of 90-100 ℃ for 2-3 h, and filtering after the water extraction; step 1.3, evaporating and concentrating the filtrate obtained in step 1.2 to a density of 1.05-1.20 g / mL, then reducing to room temperature, adding ethanol to the obtained filtrate to a concentration of 60-70%, and placing the mixture at 5-10 ℃ for 12-48 h, then centrifugally separating to reserve the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; step 1.4, adding 90-95% ethanol aqueous solution to the residue obtained in step 1.2 at a material-liquid ratio of 1:35-40 to perform reflux extraction at a temperature of 50 ℃ for 2-3 h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; step 1.5, adding 50-60% ethanol aqueous solution to the precipitate obtained in step 1.4 at a material-liquid ratio of 1:18-20 to perform extraction at a temperature of 80 ℃ for 4-5 h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; and step 1.6, combining the precipitate obtained in step 1.3 and the mixture obtained by removing the solvent from the supernatant of steps 1.3 to 1.5, uniformly stirring and drying to obtain the loquat leaf extract.

[0012] The method for preparing the composition, wherein the step 2 comprises: step 2.1, selecting loquat flowers, washing, drying and crushing into powder, and passing through a 24-30 mesh sieve; step 2.2, adding 95-98% ethanol aqueous solution to the obtained powder at a material-liquid ratio of 1:30-40 to perform reflux extraction at a temperature of 40 ℃ for 2-3 h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; step 2.3, adding 40-60% ethanol aqueous solution to the precipitate obtained in step 2.2 at a material-liquid ratio of 1:12-15 to perform reflux extraction at a temperature of 60 ℃ for 2-3 h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; step 2.4, adding 90-95% ethanol aqueous solution to the precipitate obtained in step 2.3 at a material-liquid ratio of 1:10-12 to perform ultrasonic extraction at a temperature of 80 ℃, an ultrasonic power of 600 W, an ultrasonic frequency of 100 Hz and an extraction time of 30-35 min, then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; and step 2.5, combining the mixtures obtained by removing the solvent from the supernatants of steps 2.2 to 2.4, uniformly stirring and drying to obtain the loquat flower extract.

[0013] The process for preparing the composition as claimed in claim 1, wherein step 3 comprises: step 3.1, drying and crushing the powder of the Cordyceps militaris mycelium through a 50-60 mesh sieve; step 3.2, adding distilled water to the powder obtained in step 3.1 at a solid-liquid ratio of 1:20-30, and extracting by heating and refluxing at a temperature of 75-80°C for 4-5h, and filtering after extraction; step 3.3, evaporating and concentrating the filtrate obtained in step 3.2 to a density of 1.05-1.20 g / mL, then reducing the temperature to room temperature, adding ethanol to the obtained filtrate to a concentration of 60-70%, and then placing the mixture at 5-10°C for 12-48h, and then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; step 3.4, adding distilled water to the residue obtained in step 3.2 at a solid-liquid ratio of 1:30-40, and soaking at a temperature of 4°C for 48h; step 3.5, ultrasonically extracting the mixture obtained in step 3.4 at a temperature of 30-50°C, with an ultrasonic power of 200W and an ultrasonic frequency of 100Hz, for 30-40min, and then centrifugally separating the precipitate, and concentrating and removing water from the supernatant; and step 3.6, combining the precipitate obtained in step 3.3, the mixture obtained by removing the solvent from the supernatant of step 3.3, and the mixture obtained by removing water from the supernatant of step 3.5, stirring uniformly, and drying to obtain the Cordyceps militaris extract.

[0014] The process for preparing the composition as claimed in claim 1, wherein step 4 comprises: step 4.1, washing and drying the wolfberry, and crushing the dried wolfberry into powder through a 20-30 mesh sieve; step 4.2, adding distilled water to the powder obtained in step 4.1 at a solid-liquid ratio of 1:18-20, and extracting by heating and refluxing at a temperature of 90-95°C, with each extraction time being 1.5-2h, and a total extraction time being 2-3h, and filtering after each extraction; step 4.3, evaporating and concentrating the filtrate obtained in step 4.2 to a density of 1.05-1.20 g / mL, then reducing the temperature to room temperature, adding ethanol to the obtained filtrate to a concentration of 60-70%, and then placing the mixture at 5-10°C for 12-48h, and then centrifugally separating the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure; step 4.4, adding distilled water to the residue obtained in the last filtering of step 4.2 at a solid-liquid ratio of 1:12-15, and extracting by microwave-assisted extraction at a temperature of 80-90°C, with a microwave power of 300-500W and an extraction time of 15-20min, and then centrifugally separating the precipitate, and concentrating and removing water from the supernatant; step 4.5, combining the precipitate obtained in step 4.3, the mixture obtained by removing the solvent from the supernatant of step 4.3, and the mixture obtained by removing water from the supernatant of step 4.4, stirring uniformly, and drying to obtain the wolfberry extract.

[0015] The preparation method of the composition, wherein the step 5 comprises: step 5.1, adopting the rhizoma polygonati, washing, drying and crushing into powder, and passing through a 20-30 mesh sieve; step 5.2, adding the obtained powder into 80% ethanol aqueous solution according to a material-liquid ratio of 1:12-15, and carrying out reflux extraction at a temperature of 50-60 DEG C for 2-3 hours, then centrifugally separating the precipitate, and removing the solvent of the supernatant by distillation under reduced pressure; step 5.3, adding the precipitate obtained in step 5.2 into 70% ethanol aqueous solution according to a material-liquid ratio of 1:10-12, and carrying out ultrasonic extraction at a temperature of 30-50 DEG C, an ultrasonic power of 150 W and an ultrasonic frequency of 100 Hz for 30-45 minutes, then centrifugally separating the precipitate, and removing the solvent of the supernatant by distillation under reduced pressure; and step 5.4, combining the mixtures obtained by removing the solvent of the supernatants of step 5.2 and step 5.3, stirring uniformly and drying to obtain the rhizoma polygonati extract.

[0016] The application also provides the use of the composition prepared by the method.

[0017] The composition for improving lung deficiency and having the effects of nourishing the lung and benefiting qi, the preparation method and the use thereof have the following advantages. The application provides a lung-nourishing and qi-benefiting composition which is safe and efficient, has reasonable components, is composed of natural plants and active extracts, has little harmful residues and side effects, and has biological activity.

[0018] The components are matched to form a core triangle through the main effects of relieving phlegm by flos eriobotryae, immune regulation by cordyceps militaris and cell energy activation by ginseng oligopeptide, and the introduction of gamma-aminobutyric acid (GABA) relieves airway spasm, so that the traditional lung-nourishing prescription is rarely applied. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The phenotype graph of a zebrafish obtained by experiments of the embodiment of the application under a fluorescence microscope.

[0020] Figure 2 The statistical column graph of the experimental results of the embodiment of the application. DETAILED DESCRIPTION

[0021] The specific embodiments of the application are further described below with reference to the accompanying drawings.

[0022] The composition for improving lung deficiency has the effects of nourishing the lung and benefiting qi, and is composed of the following components: loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, ginseng oligopeptide and gamma-aminobutyric acid.

[0023] Preferably, the composition is composed of the following components in parts by weight: loquat leaf extract 20-30 parts, loquat flower extract 5-15 parts, cordyceps militaris extract 25-35 parts, medlar extract 25-35 parts, polygonatum extract 5-12 parts, ginseng oligopeptide 3-12 parts and gamma-aminobutyric acid 0.5-3 parts.

[0024] More preferably, the composition is composed of the following components in parts by weight: loquat leaf extract 25 parts, loquat flower extract 10 parts, cordyceps militaris extract 30 parts, medlar extract 28 parts, polygonatum extract 8 parts, ginseng oligopeptide 6 parts and gamma-aminobutyric acid 1.5 parts.

[0025] Each raw material is wild Chinese herbal medicine, and the perennial is five years or more of medicinal materials. The materials are taken from wild rare animals and plants, and in the case where the conditions do not allow, the materials taken from artificial breeding and planting or other alternatives with equivalent and close effects can be used.

[0026] The application also provides a preparation method of the composition, wherein the method comprises: Step 1, preparing loquat leaf extract; preferably, the water extraction and alcohol precipitation method is used first.

[0027] Loquat leaf is the leaf of loquat of Rosaceae, which can be collected throughout the year, has slightly cold nature and bitter taste, and belongs to lung and stomach channels. It has the effects of clearing lung and relieving cough, regulating stomach and diuresis, and quenching thirst. It is often used for treating lung heat and phlegm cough, hemoptysis, nosebleed and stomach heat and vomiting.

[0028] Loquat leaf extract is the active substance of the dried leaf extract of loquat, and the main functional components include volatile oil, triterpenoid acid, sesquiterpene, flavonoids, polyphenols, organic acid compounds, etc. The main pharmacological effects include anti-inflammatory, antioxidant, hypoglycemic, antitussive and expectorant, antitumor, antibacterial and antiviral, and liver protection, etc.

[0029] Step 2, preparing loquat flower extract; preferably, the step-by-step alcohol extraction method is used.

[0030] Loquat flower is the flower of loquat of Rosaceae, which is collected in winter and spring and dried. It has a light taste, neutral nature and belongs to lung channel. It has the effects of dispelling wind and relieving cough, and unblocking nasal passages. It is often used for treating head wind, nasal congestion and runny nose, chronic cough and blood in sputum.

[0031] Loquat flower extract is extracted from the flowers of loquat, and contains various active components such as ursolic acid, oleanolic acid, amygdalin, etc. It has various biological activities, including anti-inflammatory, antioxidant, antibacterial and other effects.

[0032] Step 3, preparation of Cordyceps militaris extract; preferably mainly using ultrasonic water extraction method.

[0033] Cordyceps militaris is a fungus of Cordyceps genus of Cordyceps family, which is a complex composed of stroma (i.e. grass part) and sclerotium (i.e. part of the corpse of the insect). Cordyceps militaris is a fungus with both food and medicinal functions, and its protein content is as high as 40.7%. The essential amino acids contained therein are not only complete in types and sufficient in quantity, but also appropriate in proportion. Artificially cultivated Cordyceps militaris also contains rich vitamins and minerals, etc., and has good edible value. The stroma of Cordyceps militaris can be used as medicine, which is flat in nature and sweet in taste, and can benefit the lungs and kidneys, stop bleeding and reduce phlegm. It can be commonly used for pulmonary tuberculosis, weakness in the elderly, anemia and other symptoms.

[0034] Cordyceps militaris extract is extracted from artificially cultivated Cordyceps militaris fruiting body, and contains rich cordycepic acid, cordycepin and ergosterol, etc., and has the effects of expanding airway, calming, resisting various bacteria and reducing blood pressure.

[0035] Step 4, preparation of Lycium barbarum extract; preferably mainly using microwave water extraction method.

[0036] Lycium barbarum is the mature fruit of Lycium chinense or Lycium barbarum of Solanaceae. It is picked in summer and autumn, and then dried after removing the peduncle and placing in a cool place until the fruit skin wrinkles, and then sun-dried until the outer skin is hard and the fruit flesh is soft. If it rains, it can be dried with a small fire. It is sweet in nature and flat in taste, and enters the liver and kidney meridians. It has the effects of tonifying kidney, moistening lung, tonifying liver and brightening eyes. It is used for liver and kidney yin deficiency, soreness of waist and knees, dizziness, blurred vision, blurred vision, tears, emaciation, cough, diabetes and spermatorrhea.

[0037] Lycium barbarum extract is an active substance extracted from the mature fruit of Lycium chinense or Lycium barbarum of Solanaceae, and its main components are Lycium barbarum polysaccharide (content 20%-50%), betaine and carotenoids, etc., and it has the functions of immune regulation, blood glucose and blood lipid reduction, etc.

[0038] Step 5, preparation of Polygonatum extract; preferably mainly using ultrasonic alcohol extraction method.

[0039] Polygonatum is the dried rhizome of Polygonatum kingianum, Polygonatum sibiricum or Polygonatum multiflorum of Liliaceae. It is dug in spring and autumn, and then dried after removing the roots, washing, boiling or steaming until the heart is transparent, and then drying. It is sweet in taste and flat in nature; it belongs to the spleen, lung and kidney meridians. It has the effects of nourishing yin and moistening lung, tonifying spleen and invigorating qi, and nourishing kidney and filling essence. It is used for treating yin deficiency, dry cough, spleen deficiency, anorexia, diabetes, kidney deficiency, soreness of waist and knees, impotence, spermatorrhea, tinnitus, blurred vision, early graying of hair, emaciation, and leprosy.

[0040] Polygonatum extract is an active substance extracted from the rhizome of Polygonatum, which is rich in polysaccharides, flavonoids, steroidal saponins, alkaloids and other chemical components, and has the effects of anti-aging, reducing blood sugar and blood lipid, antibacterial and anti-inflammatory, and enhancing immunity.

[0041] Step 6, the obtained loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, and ginseng oligopeptide and gamma-aminobutyric acid are weighed according to the proportion, then the components are mixed uniformly, and dried, so that the water content of the mixture is less than or equal to 5% by mass percentage, then the mixture is crushed into fine powder, and then sieved through an 80-100 mesh sieve to obtain a composition, which is sterilized and sealed in bags for use.

[0042] The fine powder is the fourth level standard in the powder particle size classification system established by the Chinese Pharmacopoeia, specifically referring to the powder that can pass through a No. 5 sieve and contains not less than 95% of the powder that can pass through a No. 6 sieve.

[0043] The ginseng oligopeptide used herein is a bioactive peptide extracted from ginseng with a molecular weight below 1000 daltons. This small molecule active substance has high absorption rate and high biological activity. The ginseng oligopeptide is rich in 18 kinds of amino acids, including 8 kinds of essential amino acids, and the main active components of ginseng (ginsenosides) and trace elements, etc., and has multiple physiological activities, including anti-fatigue, anti-aging, immune enhancement, etc.

[0044] Gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid. Gamma-aminobutyric acid, as an important inhibitory neurotransmitter, widely exists in vertebrates, plants and microorganisms. It has the effects of lowering blood pressure, improving sleep, improving liver, kidney and brain function, increasing food flavor, skin moisturizing, antioxidant, etc., and is widely used in food, medicine and cosmetics fields.

[0045] The application also provides the use of the composition prepared by the method.

[0046] The composition for improving lung deficiency with the effects of nourishing the lung and benefiting qi, the preparation method and the use thereof will be further described below in combination with examples.

[0047] Example 1 A composition for improving lung deficiency with the effects of nourishing the lung and benefiting qi, comprising: loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, ginseng oligopeptide, and gamma-aminobutyric acid.

[0048] The preparation method of the composition comprises: Step 1, preparing loquat leaf extract; preferably, step 1 comprises: Step 1.1, collecting loquat leaves produced in Fujian Province, removing impurities, and removing rotten and moldy and diseased raw materials, washing and drying the obtained loquat leaves, crushing them into powder with a pulverizer, and then sieving through an 18 mesh sieve.

[0049] Step 1.2, the obtained powder was extracted with distilled water at a ratio of 1:15, and the water extraction was carried out at a temperature of 95°C for 2.5h, and then filtered.

[0050] Step 1.3, the filtrate obtained in step 1.2 was concentrated by evaporation to a density of 1.10 g / mL, and then cooled to room temperature. Ethanol was slowly added to the obtained filtrate until the ethanol concentration reached 65% (v / v). The mixture was then placed at 6°C for 30 hours, and then centrifuged to retain the precipitate. The supernatant was then distilled under reduced pressure to remove the solvent. The main components extracted were polysaccharides and flavonoids.

[0051] Step 1.4, the residue obtained by filtering in step 1.2 was extracted with 92% (v / v) ethanol aqueous solution at a ratio of 1:38, and the reflux extraction was carried out at a temperature of 50°C for 2.5h. The precipitate was then centrifuged, and the supernatant was distilled under reduced pressure to remove the solvent. The main components extracted were triterpenoid acids.

[0052] Step 1.5, the precipitate obtained in step 1.4 was extracted with 55% (v / v) ethanol aqueous solution at a ratio of 1:19, and the extraction was carried out at a temperature of 80°C for 4.5h. The precipitate was then centrifuged, and the supernatant was distilled under reduced pressure to remove the solvent. The main components extracted were polyphenols.

[0053] Step 1.6, the precipitate obtained in step 1.3 was combined with the mixture obtained by removing the solvent from the supernatant of steps 1.3 to 1.5. The mixture was stirred uniformly and then spray-dried to obtain a dry powder of loquat leaf extract.

[0054] Step 2, preparation of loquat flower extract; preferably, step 2 comprises: Step 2.1, loquat flowers that opened at the end of November and the beginning of December were selected, and impurities, as well as raw materials with mildewing and disease spots, were removed. The obtained loquat flowers were washed, dried, crushed into powder by a pulverizer, and then passed through a 26-mesh sieve.

[0055] Step 2.2, the obtained powder was extracted with 96% (v / v) ethanol aqueous solution at a ratio of 1:35, and the reflux extraction was carried out at a temperature of 40°C for 2.5h. The precipitate was then centrifuged, and the supernatant was distilled under reduced pressure to remove the solvent. The main components extracted were triterpenoids.

[0056] Step 2.3, the precipitate obtained in step 2.2 was extracted with 50% (v / v) aqueous ethanol solution at a solid-to-liquid ratio of 1 : 14 and a temperature of 60 °C by reflux extraction for 2.5 h. The precipitate was then separated by centrifugation, and the supernatant was distilled under reduced pressure to remove the solvent. The main components extracted were flavonoids, anti-inflammatory active substances, and the like.

[0057] Step 2.4, the precipitate obtained in step 2.3 was extracted with 92% (v / v) aqueous ethanol solution at a solid-to-liquid ratio of 1 : 11 and a temperature of 80 °C by ultrasonic extraction at a power of 600 W and a frequency of 100 Hz for 32 min. The precipitate was then separated by centrifugation, and the supernatant was distilled under reduced pressure to remove the solvent. The main components extracted were ursolic acid, oleanolic acid, and the like.

[0058] Step 2.5, the mixture obtained by removing the solvent from the supernatant of steps 2.2 to 2.4 was stirred uniformly and spray-dried to obtain a dried powder of loquat flower extract.

[0059] Step 3, preparation of Cordyceps militaris extract; preferably, step 3 comprises: Step 3.1, artificial cultivation of Cordyceps militaris fruiting bodies, drying of Cordyceps militaris mycelium, crushing into powder using a pulverizer, and passing through a 55-mesh sieve.

[0060] Step 3.2, extraction of the obtained powder at a solid-to-liquid ratio of 1 : 25 with distilled water at a temperature of 78 °C by heating reflux for 4.5 h, and filtration after extraction.

[0061] Step 3.3, evaporation and concentration of the filtrate obtained in step 3.2 to a density of 1.10 g / mL, then cooling to room temperature, slowly adding ethanol to the obtained filtrate to a concentration of 65%, and then centrifuging the mixture after standing at 6 °C for 24 h, retaining the precipitate, and distilling the supernatant under reduced pressure to remove the solvent. The main components extracted were cordyceps polysaccharides, cordycepin, and the like.

[0062] Step 3.4, the filter residue obtained by filtration in step 3.2 was soaked in distilled water at a solid-to-liquid ratio of 1 : 35 at a temperature of 4 °C for 48 h.

[0063] Step 3.5, ultrasonic extraction of the mixture obtained in step 3.4 at a temperature of 40 °C at a power of 200 W and a frequency of 100 Hz for 35 min, then centrifugation of the precipitate, and concentration of the supernatant to remove water. The main components extracted were cordycepic acid, adenosine, and the like.

[0064] Step 3.6, combine the precipitate obtained in step 3.3, the mixture obtained by removing the solvent from the supernatant of step 3.3, and the mixture obtained by removing the water from the supernatant of step 3.5, mix well and spray dry to obtain the dry powder of the Cordyceps militaris extract.

[0065] Step 4, preparation of the Lycium barbarum extract; preferably, step 4 comprises: Step 4.1, use high-quality Lycium barbarum, wash and dry, and crush into powder using a pulverizer, then pass through a 28-mesh sieve.

[0066] Step 4.2, add distilled water to the obtained powder at a solid-liquid ratio of 1:19, heat to reflux at a temperature of 93°C, and extract for 1.8 h each time, a total of 2 times, filter after each extraction, and combine the filtrates.

[0067] Step 4.3, evaporate and concentrate the filtrate obtained in step 4.2 to a density of 1.10 g / mL, then reduce to room temperature, slowly add ethanol to the obtained filtrate until the ethanol concentration is 65%, and then place the mixture at 6°C for 36 hours, centrifuge to retain the precipitate, and remove the solvent from the supernatant by distillation under reduced pressure. The main components extracted are Lycium barbarum polysaccharides, etc.

[0068] Step 4.4, add distilled water to the filter residue obtained after the last filtration in step 4.2 at a solid-liquid ratio of 1:14, extract by microwave assistance at a temperature of 85°C, with a microwave power of 400 W and an extraction time of 16 min, then centrifuge to separate the precipitate, and concentrate the supernatant to remove the water. The main components extracted are carotenoids, amino acids, vitamins, water-soluble antioxidants, etc.

[0069] Step 4.5, combine the precipitate obtained in step 4.3, the mixture obtained by removing the solvent from the supernatant of step 4.3, and the mixture obtained by removing the water from the supernatant of step 4.4, mix well and spray dry to obtain the dry powder of the Lycium barbarum extract.

[0070] Step 5, preparation of the Polygonatum sibiricum extract; preferably, step 5 comprises: Step 5.1, use high-quality Polygonatum sibiricum, wash and dry, and crush into powder using a pulverizer, then pass through a 24-mesh sieve.

[0071] Step 5.2, add 80% (v / v) ethanol aqueous solution to the obtained powder at a solid-liquid ratio of 1:13, extract by reflux at a temperature of 55°C for 2.5 h, then centrifuge to separate the precipitate, and remove the solvent from the supernatant by distillation under reduced pressure. The main components extracted are saponin compounds, etc.

[0072] Step 5.3, the precipitate obtained in step 5.2 is added with 70% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:11, and ultrasonic extraction is performed at a temperature of 35℃, an ultrasonic power of 150W, an ultrasonic frequency of 100Hz, and an extraction time of 40min. Then, the precipitate is centrifuged and separated, and the supernatant is distilled under reduced pressure to remove the solvent. The main components extracted are polysaccharides, flavonoids and the like.

[0073] Step 5.4, the mixture obtained after removing the solvent from the supernatants of step 5.2 and step 5.3 is combined, stirred uniformly and spray-dried to obtain a dry powder of the extract of Rhizoma Polygonati.

[0074] Step 6, the obtained extract of leaf of Eriobotrya japonica, the extract of flower of Eriobotrya japonica, the extract of Cordyceps militaris, the extract of Lycium barbarum, the extract of Rhizoma Polygonati, and the oligopeptide of Panax ginseng and gamma-aminobutyric acid are weighed according to the proportion, respectively, and then the components are mixed uniformly and dried, so that the water content of the mixture is less than or equal to 5% by mass percentage. Then, the mixture is ultrasonically broken, and the obtained fine powder is passed through an 80-100 mesh sieve by using a vibrating screen powder machine to obtain a composition, which is sterilized and sealed in a bag for standby.

[0075] The composition prepared by the method consists of the following ingredients by weight fraction: 25 parts of the extract of leaf of Eriobotrya japonica, 10 parts of the extract of flower of Eriobotrya japonica, 30 parts of the extract of Cordyceps militaris, 28 parts of the extract of Lycium barbarum, 8 parts of the extract of Rhizoma Polygonati, 6 parts of the oligopeptide of Panax ginseng, and 1.5 parts of gamma-aminobutyric acid.

[0076] The embodiment also provides an application of the composition prepared by the method, which is used for preparing an oral preparation for improving lung deficiency and having the effect of nourishing the lung and benefiting qi.

[0077] Embodiment 2 A composition for improving lung deficiency and having the effect of nourishing the lung and benefiting qi consists of the following ingredients: the extract of leaf of Eriobotrya japonica, the extract of flower of Eriobotrya japonica, the extract of Cordyceps militaris, the extract of Lycium barbarum, the extract of Rhizoma Polygonati, the oligopeptide of Panax ginseng, and gamma-aminobutyric acid.

[0078] The preparation method of the composition comprises: Step 1, preparing the extract of leaf of Eriobotrya japonica; preferably, step 1 comprises: Step 1.1, collecting the leaf of Eriobotrya japonica produced in Fujian Province, removing impurities and the raw materials with mildew and disease spots, washing and drying the obtained leaf of Eriobotrya japonica, crushing it into powder by using a pulverizer, and then passing it through a 16-mesh sieve.

[0079] Step 1.2, adding distilled water to the obtained powder at a solid-liquid ratio of 1:12 for extraction at a temperature of 90℃ for 2h, and then filtering after water extraction.

[0080] Step 1.3, the filtrate obtained in step 1.2 was concentrated by evaporation to a density of 1.05 g / mL, then cooled to room temperature, and ethanol was slowly added to the filtrate to a concentration of 60% (v / v), the mixture was allowed to stand at 5°C for 12 hours, then centrifuged to retain the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0081] Step 1.4, the residue obtained by filtration in step 1.2 was extracted with 90% (v / v) aqueous ethanol at a solid-to-liquid ratio of 1:35 by refluxing at 50°C for 2 hours, then centrifuged to separate the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0082] Step 1.5, the precipitate obtained in step 1.4 was extracted with 50% (v / v) aqueous ethanol at a solid-to-liquid ratio of 1:18 by soaking at 80°C for 4 hours, then centrifuged to separate the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0083] Step 1.6, the precipitate obtained in step 1.3 was combined with the mixture obtained by removing the solvent from the supernatant of steps 1.3 to 1.5, stirred uniformly, and spray-dried to obtain a dried powder of loquat leaf extract.

[0084] Step 2, preparation of loquat flower extract; preferably, step 2 comprises: Step 2.1, loquat flowers that open at the end of November or the beginning of December were selected, impurities, as well as moldy and diseased raw materials were removed, the obtained loquat flowers were washed and dried, crushed into powder using a pulverizer, and then passed through a 24-mesh sieve.

[0085] Step 2.2, the obtained powder was extracted with 95% (v / v) aqueous ethanol at a solid-to-liquid ratio of 1:30 by refluxing at 40°C for 2 hours, then centrifuged to separate the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0086] Step 2.3, the precipitate obtained in step 2.2 was extracted with 40% (v / v) aqueous ethanol at a solid-to-liquid ratio of 1:12 by refluxing at 60°C for 2 hours, then centrifuged to separate the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0087] Step 2.4, the precipitate obtained in step 2.3 was extracted with 90% (v / v) aqueous ethanol at a solid-to-liquid ratio of 1:10 by ultrasonic extraction at 80°C, with an ultrasonic power of 600 W and an ultrasonic frequency of 100 Hz for 30 minutes, then centrifuged to separate the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0088] Step 2.5, the mixture obtained after removing the solvent from the supernatant of step 2.2 to step 2.4 is combined, stirred uniformly and spray-dried to obtain the loquat flower extract in the form of dry powder.

[0089] Step 3, preparation of the Cordyceps militaris extract; preferably, step 3 comprises: Step 3.1, using the artificially cultivated Cordyceps militaris fruiting body, the Cordyceps militaris mycelium is dried and broken into powder by a pulverizer and then passed through a 50-mesh sieve.

[0090] Step 3.2, the obtained powder is added with distilled water at a solid-liquid ratio of 1:20, heated to reflux at a temperature of 75°C for 4h, and filtered after extraction.

[0091] Step 3.3, the filtrate obtained in step 3.2 is evaporated and concentrated to a density of 1.05g / mL, then cooled to room temperature, and ethanol is slowly added to the obtained filtrate until the ethanol concentration is 60%, and then the mixture is placed at 5°C for 12h, centrifuged to retain the precipitate, and the supernatant is distilled under reduced pressure to remove the solvent.

[0092] Step 3.4, the residue obtained by filtering in step 3.2 is added with distilled water at a solid-liquid ratio of 1:30, and soaked at a temperature of 4°C for 48h.

[0093] Step 3.5, the mixture obtained in step 3.4 is ultrasonically extracted at a temperature of 30°C, with an ultrasonic power of 200W and an ultrasonic frequency of 100Hz, for 30min, then centrifuged to separate the precipitate, and the supernatant is concentrated to remove water.

[0094] Step 3.6, the precipitate obtained in step 3.3 is combined with the mixture obtained by removing the solvent from the supernatant of step 3.3 and the mixture obtained by removing water from the supernatant of step 3.5, stirred uniformly and spray-dried to obtain the Cordyceps militaris extract in the form of dry powder.

[0095] Step 4, preparation of the Lycium barbarum extract; preferably, step 4 comprises: Step 4.1, using high-quality Lycium barbarum, washed and dried, and broken into powder by a pulverizer and then passed through a 20-mesh sieve.

[0096] Step 4.2, the obtained powder is added with distilled water at a solid-liquid ratio of 1:18, heated to reflux at a temperature of 90°C for 1.5h each time, for a total of 2 times, and filtered after each extraction, and the filtrates are combined.

[0097] Step 4.3, the filtrate obtained in step 4.2 was concentrated by evaporation to a density of 1.05 g / mL, then the resulting filtrate was cooled to room temperature, and ethanol was slowly added to the filtrate until the ethanol concentration reached 60%, and the mixture was then allowed to stand at 5°C for 12 hours, after which the mixture was centrifuged to retain the precipitate and the supernatant was distilled under reduced pressure to remove the solvent.

[0098] Step 4.4, the residue obtained by the last filtration in step 4.2 was added with distilled water at a solid-liquid ratio of 1:12, and the mixture was extracted by microwave assistance at a temperature of 80°C, a microwave power of 300W, and an extraction time of 15 min, then the precipitate was centrifuged and the supernatant was concentrated to remove water.

[0099] Step 4.5, the precipitate obtained in step 4.3 was combined with the mixture obtained by removing the solvent from the supernatant of step 4.3 and the mixture obtained by removing water from the supernatant of step 4.4, and the mixture was stirred uniformly and spray-dried to obtain a dry powder of wolfberry extract.

[0100] Step 5, preparation of polygonatum extract; preferably, step 5 comprises: Step 5.1, high-quality polygonatum was washed, dried, and crushed into powder using a crusher, and then passed through a 20-mesh sieve.

[0101] Step 5.2, the obtained powder was added with 80% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:12, and the mixture was extracted by reflux at a temperature of 50°C for 2 h, then the precipitate was centrifuged and the supernatant was distilled under reduced pressure to remove the solvent.

[0102] Step 5.3, the precipitate obtained in step 5.2 was added with 70% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:10, and the mixture was extracted by ultrasonic at a temperature of 30°C, an ultrasonic power of 150W, and an ultrasonic frequency of 100Hz for 30 min, then the precipitate was centrifuged and the supernatant was distilled under reduced pressure to remove the solvent.

[0103] Step 5.4, the mixtures obtained by removing the solvent from the supernatants of step 5.2 and step 5.3 were combined, stirred uniformly, and spray-dried to obtain a dry powder of polygonatum extract.

[0104] Step 6, the obtained loquat leaf extract, loquat flower extract, cordyceps militaris extract, wolfberry extract, polygonatum extract, and ginseng oligopeptide and gamma-aminobutyric acid were weighed according to the proportions, respectively, then the ingredients were mixed uniformly, and dried to make the water content of the mixture less than or equal to 5% by mass percentage, then the mixture was ultrasonically broken, and the obtained fine powder was passed through an 80-100 mesh sieve using a vibrating screen powder machine to obtain a composition, which was sterilized, bagged, and sealed for use.

[0105] The composition prepared by the method consists of, by weight fraction, 20 parts of loquat leaf extract, 5 parts of loquat flower extract, 25 parts of cordyceps militaris extract, 25 parts of medlar extract, 5 parts of polygonatum extract, 3 parts of ginseng oligopeptide, and 0.5 parts of gamma-aminobutyric acid.

[0106] The present embodiment also provides the use of the composition prepared by the method, which is used for preparing an oral preparation for improving lung deficiency and having the effect of nourishing the lung and benefiting qi.

[0107] Embodiment 3 A composition for improving lung deficiency and having the effect of nourishing the lung and benefiting qi consists of loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, ginseng oligopeptide, and gamma-aminobutyric acid.

[0108] The preparation method of the composition comprises: Step 1, preparing loquat leaf extract; preferably, step 1 comprises: Step 1.1, collecting loquat leaves produced in Fujian Province, removing impurities and raw materials with mildew and disease spots, washing and drying the obtained loquat leaves, crushing them into powder with a pulverizer, and then passing through a 20-mesh sieve.

[0109] Step 1.2, adding distilled water to the obtained powder at a solid-liquid ratio of 1:18 for extraction at a temperature of 100℃ for 3h, and then filtering after water extraction.

[0110] Step 1.3, evaporating and concentrating the filtrate obtained in step 1.2 to a density of 1.20 g / mL, then reducing the temperature to room temperature, slowly adding ethanol to the obtained filtrate until the ethanol concentration is 70% (v / v), placing the mixture at 10℃ for 48h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by reduced-pressure distillation.

[0111] Step 1.4, adding 95% (v / v) ethanol aqueous solution to the filter residue obtained in step 1.2 at a solid-liquid ratio of 1:40 for reflux extraction at a temperature of 50℃ for 3h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by reduced-pressure distillation.

[0112] Step 1.5, adding 60% (v / v) ethanol aqueous solution to the precipitate obtained in step 1.4 at a solid-liquid ratio of 1:20 for extraction at a temperature of 80℃ for 5h, then centrifugally separating the precipitate, and removing the solvent from the supernatant by reduced-pressure distillation.

[0113] Step 1.6, combining the precipitate obtained in step 1.3 with the mixture obtained by removing the solvent from the supernatant of steps 1.3 to 1.5, stirring uniformly, and then spray-drying to obtain dry powder of loquat leaf extract.

[0114] Step 2, preparing the loquat flower extract; preferably, step 2 comprises: Step 2.1, selecting loquat flowers that open at the end of November to the beginning of December, removing impurities and raw materials that are wilted, moldy and have spots, washing the obtained loquat flowers and drying them, crushing them into powder using a crusher, and then passing them through a 30-mesh sieve.

[0115] Step 2.2, adding the obtained powder to 98% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:40, refluxing at a temperature of 40°C for 3h, centrifuging the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure.

[0116] Step 2.3, adding the precipitate obtained in step 2.2 to 60% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:15, refluxing at a temperature of 60°C for 3h, centrifuging the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure.

[0117] Step 2.4, adding the precipitate obtained in step 2.3 to 95% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:12, ultrasonic extracting at a temperature of 80°C, an ultrasonic power of 600W, an ultrasonic frequency of 100Hz, and for 35min, centrifuging the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure.

[0118] Step 2.5, combining the mixtures obtained by removing the solvent from the supernatants of steps 2.2 to 2.4, stirring them uniformly, and spray drying to obtain a loquat flower extract in the form of dry powder.

[0119] Step 3, preparing the cordyceps militaris extract; preferably, step 3 comprises: Step 3.1, drying cordyceps militaris mycelium using artificial cultivation of cordyceps militaris fruiting bodies, crushing them into powder using a crusher, and then passing them through a 60-mesh sieve.

[0120] Step 3.2, adding the obtained powder to distilled water at a solid-liquid ratio of 1:30, heating and refluxing at a temperature of 80°C for 5h, and filtering after the extraction.

[0121] Step 3.3, evaporating and concentrating the filtrate obtained in step 3.2 to a density of 1.20 g / mL, then lowering the temperature to room temperature, slowly adding ethanol to the obtained filtrate until the ethanol concentration is 70%, and then centrifuging the mixture after it is placed at 10°C for 48h, retaining the precipitate, and removing the solvent from the supernatant by distillation under reduced pressure.

[0122] Step 3.4, adding the filter residue obtained by filtering in step 3.2 to distilled water at a solid-liquid ratio of 1:40, and soaking at a temperature of 4°C for 48h.

[0123] Step 3.5, the mixture obtained in step 3.4 was subjected to ultrasonic extraction at a temperature of 50 °C, ultrasonic power of 200 W, ultrasonic frequency of 100 Hz, and extraction time of 40 min, then the precipitate was separated by centrifugation, and the supernatant was concentrated to remove water.

[0124] Step 3.6, the precipitate obtained in step 3.3 was combined with the mixture obtained by removing the solvent from the supernatant of step 3.3 and the mixture obtained by removing water from the supernatant of step 3.5, stirred uniformly and subjected to spray drying to obtain Cordyceps militaris extract in dry powder form.

[0125] Step 4, preparation of wolfberry extract; preferably, step 4 comprises: Step 4.1, high-quality wolfberry was used, washed, dried, crushed into powder by a crusher, and then passed through a 30-mesh sieve.

[0126] Step 4.2, the obtained powder was added with distilled water at a solid-liquid ratio of 1:20, heated to reflux at a temperature of 95 °C, and extracted for 2 h each time, a total of 3 times, and filtration was performed after each extraction, and the filtrates were combined.

[0127] Step 4.3, the filtrate obtained in step 4.2 was evaporated and concentrated to a density of 1.20 g / mL, then cooled to room temperature, and ethanol was slowly added to the obtained filtrate until the ethanol concentration was 70%, and then the mixture was placed at 10 °C for 48 h, then centrifuged to retain the precipitate, and the supernatant was distilled under reduced pressure to remove the solvent.

[0128] Step 4.4, the filter residue obtained by the last filtration in step 4.2 was added with distilled water at a solid-liquid ratio of 1:15, subjected to microwave-assisted extraction at a temperature of 90 °C, microwave power of 500 W, and extraction time of 20 min, then the precipitate was separated by centrifugation, and the supernatant was concentrated to remove water.

[0129] Step 4.5, the precipitate obtained in step 4.3 was combined with the mixture obtained by removing the solvent from the supernatant of step 4.3 and the mixture obtained by removing water from the supernatant of step 4.4, stirred uniformly and subjected to spray drying to obtain wolfberry extract in dry powder form.

[0130] Step 5, preparation of polygonatum extract; preferably, step 5 comprises: Step 5.1, high-quality polygonatum was used, washed, dried, crushed into powder by a crusher, and then passed through a 30-mesh sieve.

[0131] Step 5.2, the obtained powder was added with 80% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:15, subjected to reflux extraction at a temperature of 60 °C for 3 h, then the precipitate was separated by centrifugation, and the supernatant was distilled under reduced pressure to remove the solvent.

[0132] Step 5.3, the precipitate obtained in step 5.2 was added with 70% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:12, and ultrasonic extraction was carried out at a temperature of 50℃, an ultrasonic power of 150W, an ultrasonic frequency of 100Hz, and an extraction time of 45min. Then, the precipitate was centrifuged and separated, and the supernatant was distilled under reduced pressure to remove the solvent.

[0133] Step 5.4, the mixture obtained by removing the solvent from the supernatant of step 5.2 and step 5.3 was stirred uniformly and then spray-dried to obtain a dry powder of the polygonatum extract.

[0134] Step 6, the obtained loquat leaf extract, loquat flower extract, cordyceps militaris extract, medlar extract, polygonatum extract, ginseng oligopeptide and gamma-aminobutyric acid were weighed according to the proportion, then mixed uniformly, and dried to make the water content of the mixture less than or equal to 5% by mass percentage. Then, the mixture was ultrasonically broken, and the obtained fine powder was passed through an 80-100 mesh sieve by using a vibrating screen powder machine to obtain a composition, which was sterilized and sealed in a bag for standby.

[0135] The composition prepared by the method consists of the following ingredients by weight fraction: loquat leaf extract 30 parts, loquat flower extract 15 parts, cordyceps militaris extract 35 parts, medlar extract 35 parts, polygonatum extract 12 parts, ginseng oligopeptide 12 parts, and gamma-aminobutyric acid 3 parts.

[0136] The embodiment also provides the application of the composition prepared by the method, which is used for preparing an oral preparation for improving lung deficiency and having the effect of nourishing the lung and benefiting qi.

[0137] The raw materials used in the above embodiments meet the corresponding national standards.

[0138] Example 4 The composition prepared in Example 1 was used for experimental detection, and the specific content is as follows.

[0139] I. Overview of experimental methods.

[0140] 1. Detection principle.

[0141] The swim bladder of zebrafish is similar to mammalian alveoli in terms of organ evolution, tissue structure, and biology.

[0142] Establishment of viral pneumonia model: Inject polyinosinic-polycytidylic acid (poly (i:c)) to establish the viral pneumonia model of zebrafish. The double-stranded RNA structure of poly (i:c) is very similar to the double-stranded RNA formed during viral or viral replication, so poly (i:c) is similar to some viruses and has a strong interferon induction effect. By using transgenic neutrophil green fluorescent zebrafish, neutrophils in zebrafish can be directly observed under a fluorescence microscope, and neutrophil extravasation can be video recorded at different time points. The significant increase in neutrophils in the swim bladder confirms the establishment of the viral pneumonia model of zebrafish.

[0143] 2. Materials and methods.

[0144] 2.1 Experimental instruments and reagents.

[0145] Stereo microscope (SZX7, Olympus, Japan).

[0146] Polyinosinic-polycytidylic acid (poly (i:c)) (CAS: 24939-03-5).

[0147] 2.2 Detection system and sample size.

[0148] Experimental system: neutrophil fluorescent zebrafish.

[0149] Zebrafish age: 5 days after fertilization.

[0150] Sample size of each experimental group: 30 per well, 1 well per group.

[0151] Adult fish feeding and breeding method: according to the standard feeding and breeding method.

[0152] The type of zebrafish used in this experiment is Tg (lyz: EGFP). All zebrafish are raised in a recirculating aquarium system with a temperature of 28±1℃, a salinity of 0.03%-0.04%, and a pH of 7.2-7.6. The lighting system is alternated according to 14h / 10h (light / dark). Brine shrimp is fed in the morning and evening every day. Adult fish can mate once every other week. Embryos are collected for experimental use. In order to obtain zebrafish embryos, adult zebrafish are placed in a mating tank at a ratio of 1:1 / 2:1 the day before the experiment. The next morning, the light is turned on and the partition is removed, allowing the male and female zebrafish to naturally mate and lay eggs. In order to ensure the quality of the embryos, the process of collecting embryos should be controlled within 30 minutes. The obtained embryos are stored in E3 medium, and the culture dish contains 25mL of 0.3% methylene blue 1×E3 solution, ensuring that 50 embryos are placed per 100mm 2

[0153] 3. Detection process.

[0154] ​The test zebrafish were divided into four groups, namely a normal control group, a model control group, a positive control (taking anti-inflammatory drugs) group and a sample treatment group. Among them, the model control group, the positive control (taking anti-inflammatory drugs) group and the sample treatment group were injected with the same amount of poly I:C to establish a viral pneumonia model.

[0155] The positive control (taking anti-inflammatory drugs) group ingested anti-pneumonia anti-inflammatory drugs such as indomethacin while being injected with poly I:C. The sample treatment group ingested the composition prepared in Example 1 while being injected with poly I:C.

[0156] After the zebrafish took anti-pneumonia anti-inflammatory drugs and the composition prepared in Example 1 for a period of time, the zebrafish were photographed under a fluorescence microscope, and the number of neutrophils was counted.

[0157] II. Data processing method.

[0158] SPSS 26 software was used for analysis, GraphPad Prism 8 was used for plotting, the data were represented by mean ± standard deviation, and the experimental results were subjected to normal distribution test using SPSS software. If the data were normally distributed, T parameter test or analysis of variance (ANOVA) was used for significance test. If the data were not normally distributed, non-parametric test was used (P value <0.05, indicating statistically significant difference).

[0159] III. Determination basis.

[0160] On the basis that the test meets the effectiveness, the number of neutrophils in the model control group was significantly increased (P<0.05) compared with the blank control group, indicating that the modeling was successful.

[0161] Compared with the model control group, the number of neutrophils in the sample treatment group was significantly reduced (P<0.05), indicating that the sample had the efficacy of anti-viral pneumonia.

[0162] Test results.

[0163] The cell phenotype of the zebrafish obtained by the experiment under a fluorescence microscope is shown in Figure 1 The statistical results of the number of neutrophils are shown in Table 1 below.

[0164] Table 1. Neutrophil number statistics table (number).

[0165] Among them, the number of neutrophils in the model control group was significantly increased (P<0.001) compared with the blank control group. The number of neutrophils in the sample treatment group was significantly reduced (P<0.05) compared with the model control group.

[0166] The results of the efficacy experiment of the composition sample prepared in Example 1, i.e., the statistical column chart of the number of neutrophils (pieces) is shown in Figure Figure 2 *: P < 0.05; **: P < 0.01; ***: P < 0.001; The more, the smaller the P value represents the more significant difference.

[0167] The final experimental results are shown in Table 2 below.

[0168] Table 2. Experimental results table.

[0169] Note: Through the pre-experiment, it is obtained that the sample treatment group of 500 mg / L has no significant difference compared with the blank control group, so 500 mg / L is the maximum safe concentration.

[0170] Conclusion: After the zebrafish model of viral pneumonia is treated with the composition sample prepared in Example 1, the number of neutrophils is significantly reduced (P < 0.05) compared with the model control group, which indicates that the composition has the efficacy of resisting viral pneumonia.

[0171] The composition for improving lung deficiency, which has the efficacy of nourishing the lung and benefiting qi, and the preparation method and application thereof, are provided. The obtained composition is composed of natural plants and active extracts. Through the main effects of relieving phlegm by leafing of loquat flowers, immune regulation by cordyceps militaris, and cell energy activation by ginseng oligopeptide, a core triangle is formed. The introduction of GABA can relieve airway spasm, achieve the effects of moistening the lung to stop cough, resolving phlegm to eliminate inflammation, tonifying qi and nourishing yin, and enhancing immunity, and is suitable for improving lung qi deficiency, and syndrome of deficiency of both qi and yin.

[0172] Although the content of the present application has been described in detail through the above preferred embodiments, it should be recognized that the above description should not be considered as a limitation of the present application. After reading the above content, various modifications and alternatives of the present application will be apparent to those skilled in the art. Therefore, the protection scope of the present application should be defined by the appended claims.​

Claims

1. A composition for improving lung deficiency and having the effect of nourishing the lungs and replenishing qi, characterized in that, The composition consists of the following ingredients: loquat leaf extract, loquat flower extract, cordyceps militaris extract, wolfberry extract, polygonatum extract, ginseng oligopeptide, and gamma-aminobutyric acid.

2. The composition as described in claim 1, which improves lung deficiency and has the effect of nourishing the lungs and replenishing qi, is characterized in that, The composition comprises, by weight, the following components: 20-30 parts loquat leaf extract, 5-15 parts loquat flower extract, 25-35 parts cordyceps extract, 25-35 parts wolfberry extract, 5-12 parts polygonatum extract, 3-12 parts ginseng oligopeptide, and 0.5-3 parts γ-aminobutyric acid.

3. The composition as described in claim 2, which improves lung deficiency and has the effect of nourishing the lungs and replenishing qi, is characterized in that, The composition comprises the following components by weight: 25 parts loquat leaf extract, 10 parts loquat flower extract, 30 parts cordyceps extract, 28 parts wolfberry extract, 8 parts polygonatum extract, 6 parts ginseng oligopeptide, and 1.5 parts γ-aminobutyric acid.

4. A method for preparing the composition according to any one of claims 1 to 3, characterized in that, The method includes: Step 1: Prepare loquat leaf extract; Step 2: Prepare loquat flower extract; Step 3: Prepare Cordyceps militaris extract; Step 4: Prepare wolfberry extract; Step 5: Prepare Polygonatum extract; Step 6: Weigh the obtained loquat leaf extract, loquat flower extract, cordyceps militaris extract, wolfberry extract, polygonatum extract, ginseng oligopeptide and γ-aminobutyric acid according to the specified proportions. Then mix the components evenly and dry them. Make the water content of the mixture less than or equal to 5% by mass percentage. Then crush the mixture into fine powder and pass it through an 80-100 mesh sieve to obtain the composition. After sterilization, pack it into bags and seal it for later use.

5. The method for preparing the composition according to claim 4, characterized in that, Step 1 includes: Step 1.1: Collect loquat leaves, wash them, dry them, and crush them into powder, then pass them through a 16-20 mesh sieve; Step 1.2: Add distilled water to the obtained powder at a material-to-liquid ratio of 1:12-18 for extraction. The water extraction is carried out at a temperature of 90-100℃ for 2-3 hours, and then filtered after extraction. Step 1.3: Evaporate and concentrate the filtrate obtained in Step 1.2 to a density of 1.05-1.20 g / mL, then cool to room temperature, add ethanol to the filtrate to a concentration of 60-70%, let the mixture stand at 5-10℃ for 12-48 hours, centrifuge to separate the precipitate, and remove the solvent by vacuum distillation of the supernatant. Step 1.4: Add 90-95% ethanol aqueous solution to the filter residue obtained in step 1.2 at a material-to-liquid ratio of 1:35-40, and reflux extract at 50°C for 2-3 hours. Then centrifuge to separate the precipitate and remove the solvent by vacuum distillation of the supernatant. Step 1.5: Add the precipitate obtained in step 1.4 to a 50-60% ethanol aqueous solution at a material-to-liquid ratio of 1:18-20, extract at 80°C for 4-5 hours, then centrifuge to separate the precipitate, and remove the solvent from the supernatant by vacuum distillation. Step 1.6: Combine the precipitate obtained in Step 1.3 with the mixture obtained after removing the solvent from the supernatant of Steps 1.3 to 1.5, stir evenly and dry to obtain loquat leaf extract.

6. The method for preparing the composition according to claim 4, characterized in that, Step 2 includes: Step 2.1: Select loquat flowers, wash them, dry them, and crush them into powder, then pass them through a 24-30 mesh sieve; Step 2.2: Add the obtained powder to a 95-98% ethanol aqueous solution at a material-to-liquid ratio of 1:30-40, and reflux extract at 40°C for 2-3 hours. Then centrifuge to separate the precipitate, and remove the solvent from the supernatant by vacuum distillation. Step 2.3: Add the precipitate obtained in step 2.2 to a 40-60% ethanol aqueous solution at a material-to-liquid ratio of 1:12-15, and reflux extract at 60°C for 2-3 hours. Then centrifuge to separate the precipitate and remove the solvent from the supernatant by vacuum distillation. Step 2.4: Add the precipitate obtained in step 2.3 to a 90-95% ethanol aqueous solution at a material-to-liquid ratio of 1:10-12, and perform ultrasonic extraction at 80°C with an ultrasonic power of 600W and an ultrasonic frequency of 100Hz for 30-35 minutes. Then, centrifuge to separate the precipitate and remove the solvent from the supernatant by vacuum distillation. Step 2.5: Combine the mixtures obtained after removing the solvent from the supernatants of Steps 2.2 to 2.4, stir evenly and dry to obtain loquat flower extract.

7. The method for preparing the composition according to claim 4, characterized in that, Step 3 includes: Step 3.1: Use Cordyceps militaris mycelium, dry and crush it into powder, and pass it through a 50-60 mesh sieve; Step 3.2: Add distilled water to the obtained powder at a material-to-liquid ratio of 1:20-30, heat and reflux at 75-80℃ for 4-5 hours, and filter after extraction. Step 3.3: Evaporate and concentrate the filtrate obtained in step 3.2 to a density of 1.05-1.20 g / mL, then cool to room temperature, add ethanol to the filtrate to a concentration of 60-70%, let the mixture stand at 5-10℃ for 12-48 hours, centrifuge to separate the precipitate, and remove the solvent by vacuum distillation of the supernatant. Step 3.4: Add distilled water to the filter residue obtained in step 3.2 at a material-to-liquid ratio of 1:30-40, and soak at 4 ℃ for 48 hours. Step 3.5: The mixture obtained in step 3.4 is subjected to ultrasonic extraction at a temperature of 30-50℃, with an ultrasonic power of 200W, an ultrasonic frequency of 100Hz, and an extraction time of 30-40min. Then, the precipitate is separated by centrifugation, and the supernatant is concentrated to remove water. Step 3.6: Combine the precipitate obtained in step 3.3 with the mixture obtained by removing the solvent from the supernatant in step 3.3 and the mixture obtained by removing the water from the supernatant in step 3.5, stir evenly and dry to obtain Cordyceps militaris extract.

8. The method for preparing the composition according to claim 4, characterized in that, Step 4 includes: Step 4.1: Use goji berries, wash them, dry them, and crush them into powder, then pass them through a 20-30 mesh sieve; Step 4.2: Add distilled water to the obtained powder at a material-to-liquid ratio of 1:18-20, and extract by reflux at a temperature of 90-95℃. Each extraction takes 1.5-2 hours, and the extraction is repeated 2-3 times. After each extraction, filter the mixture and combine the filtrates. Step 4.3: Evaporate and concentrate the filtrate obtained in step 4.2 to a density of 1.05-1.20 g / mL, then cool to room temperature, add ethanol to the filtrate to a concentration of 60-70%, let the mixture stand at 5-10℃ for 12-48 hours, centrifuge to separate the precipitate, and remove the solvent by vacuum distillation of the supernatant. Step 4.4: Add distilled water to the filter residue obtained in the final filtration of step 4.2 at a material-to-liquid ratio of 1:12-15, and extract it with microwave assistance at a temperature of 80-90℃, with a microwave power of 300-500W and an extraction time of 15-20min. Then, centrifuge to separate the precipitate and concentrate the supernatant to remove water. Step 4.5: Combine the precipitate obtained in step 4.3 with the mixture obtained by removing the solvent from the supernatant in step 4.3 and the mixture obtained by removing the water from the supernatant in step 4.4, stir evenly and dry to obtain wolfberry extract.

9. The method for preparing the composition according to claim 4, characterized in that, Step 5 includes: Step 5.1: Use Polygonatum sibiricum, wash it, dry it and crush it into powder, and pass it through a 20-30 mesh sieve; Step 5.2: Add the obtained powder to an 80% ethanol aqueous solution at a material-to-liquid ratio of 1:12-15, and reflux extract at a temperature of 50-60℃ for 2-3 hours. Then, centrifuge to separate the precipitate and remove the solvent from the supernatant by vacuum distillation. Step 5.3: Add the precipitate obtained in step 5.2 to a 70% ethanol aqueous solution at a material-to-liquid ratio of 1:10-12, and perform ultrasonic extraction at a temperature of 30-50℃, with an ultrasonic power of 150W, an ultrasonic frequency of 100Hz, and an extraction time of 30-45min. Then, centrifuge to separate the precipitate, and remove the solvent from the supernatant by vacuum distillation. Step 5.4: Combine the mixtures obtained after removing the solvent from the supernatants of Steps 5.2 and 5.3, stir evenly and dry to obtain Polygonatum extract.

10. An application of the composition prepared by the method of claim 4, characterized in that, The composition is used to prepare an oral preparation that improves lung deficiency and has the effect of nourishing the lungs and replenishing qi.

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