Rapid cell lysis buffer solution and preparation method thereof

Abstract

The invention discloses a rapid cell lysis buffer solution and a preparation method thereof, and belongs to the technical field of cell lysis buffer solution preparation. The barrel cover is detachably connected to the mixing barrel, a connecting cylinder is installed on the barrel cover, the spiral conveying blade is driven to rotate through rotation of the first rotating rod, raw materials in the conveying pipe are conveyed to the lower pipe through rotation of the spiral conveying blade, and the raw materials are fed into the connecting cylinder through the lower pipe; meanwhile, the output end of a first motor rotates to drive a second rotating rod and transmission gears to rotate, the second rotating rod rotates to drive grinding rollers to rotate, and due to the fact that the two transmission gears are meshed, the rotating directions of the two grinding rollers are opposite, so that solids are conveniently ground through the two grinding rollers; therefore, the solid and the liquid can be stirred and mixed more uniformly, and the quality of the prepared cell lysis buffer solution is optimized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell lysis buffer preparation, more particularly to a rapid cell lysis buffer and a preparation method thereof. BACKGROUND

[0002] The rapid cell lysis buffer is an optimized mixture of chemical reagents, and the core design purpose is to efficiently and rapidly (usually within a few minutes to tens of minutes) destroy the cell membrane and nuclear membrane, thereby releasing the biological macromolecules (such as proteins, nucleic acids, enzymes, etc.) in the cell, while maintaining the natural activity and integrity of these target molecules as much as possible and inhibiting the degradation process.

[0003] The rapid cell lysis buffer is a multifunctional and high-efficiency laboratory basic reagent, which realizes the rapid destruction of cells and the efficient recovery of target biological molecules through the synergistic effect of chemical detergents and various protective reagents, and is an indispensable tool in modern molecular biology and cell biology research.

[0004] In the prior art, when preparing the cell lysis buffer, the solid raw materials are easily fused unevenly, resulting in poor quality of the prepared cell lysis buffer, and therefore the present application provides a rapid cell lysis buffer and a preparation method thereof. SUMMARY

[0005] Technical problems to be solved

[0006] In view of the problems in the prior art, the present application aims to provide a rapid cell lysis buffer and a preparation method thereof, which can drive the spiral conveying blade to rotate by the rotation of the first rotating rod, transport the raw materials in the conveying pipe to the lower pipe by the rotation of the spiral conveying blade, and pour the raw materials into the connecting cylinder through the lower pipe; at the same time, the output end of the first motor drives the second rotating rod and the transmission gear to rotate, and the rotation of the second rotating rod drives the grinding rollers to rotate, and since the two transmission gears are engaged, the rotation directions of the two grinding rollers are opposite, so that the two grinding rollers can be used to crush the solid, so that the solid and liquid can be mixed more uniformly, and the quality of the prepared cell lysis buffer is optimized.

[0007] Technical scheme

[0008] To solve the above problems, the present application adopts the following technical scheme:

[0009] The rapid cell lysis buffer comprises the following raw materials by weight: Tris-HCl buffer (pH 7.5) 50-70 parts, sodium chloride 8-12 parts, disodium ethylenediaminetetraacetate 1-3 parts, glycerol 15-25 parts, NP-40 detergent 3-7 parts, sodium dodecyl sulfate 0.5-1.5 parts, and protease inhibitor mixture 0.5-1.5 parts.

[0010] The application relates to a preparation method of a rapid cell lysis buffer, and discloses a preparation method of a rapid cell lysis buffer.

[0011] A mixing barrel;

[0012] A barrel cover which is detachably connected to the mixing barrel, wherein a connecting cylinder is arranged on the barrel cover, a top cover is detachably connected in the connecting cylinder, and a control panel is arranged at the front end of the connecting cylinder;

[0013] A thermometer which is arranged in the barrel cover;

[0014] A second motor which is arranged at the bottom of the mixing barrel, and the output end of the second motor is movably penetrated through the mixing barrel, a third rotating rod is fixedly connected to the output end of the second motor, a plurality of stirring rods are fixedly connected to the surface of the third rotating rod, and stirring blades are fixedly connected to the surface of the third rotating rod;

[0015] A feeding mechanism which is arranged on the mixing barrel and connected to the barrel cover;

[0016] A heating mechanism which is arranged outside the mixing barrel and connected to the mixing barrel.

[0017] As a preferred scheme of the application, the feeding mechanism comprises a feeding assembly and a liquid adding assembly, and the feeding assembly and the liquid adding assembly are arranged on the barrel cover and connected to each other;

[0018] The liquid adding assembly comprises a liquid inlet pipe and a liquid inlet valve, the liquid inlet pipe is arranged on the barrel cover, and the liquid inlet valve is arranged on the surface of the liquid inlet pipe.

[0019] As a preferred scheme of the application, the feeding assembly comprises two first supporting plates, a conveying pipe, an upper pipe, a lower pipe, a first rotating rod and spiral conveying blades, the two first supporting plates are fixedly connected to the connecting cylinder and the barrel cover respectively, the conveying pipe is fixedly connected to the two first supporting plates, the lower pipe is fixedly connected to the bottom of the conveying pipe and connected to the top cover in communication, the upper pipe is arranged on the conveying pipe, the first rotating rod is rotatably connected to the conveying pipe and movably penetrated through the right side of the conveying pipe at the right end, and the spiral conveying blades are fixedly connected to the surface of the first rotating rod.

[0020] As a preferred scheme of the present application, the feeding assembly further comprises a protective shell, grinding rollers, second rotating rods, transmission gears and a first motor, the protective shell is installed on the right side of the connecting cylinder, the grinding rollers, the second rotating rods and the transmission gears are all provided in two, the two grinding rollers are both rotationally connected in the connecting cylinder, the two second rotating rods are both fixedly connected on the right sides of the two grinding rollers and movably penetrate through the connecting cylinder, the two transmission gears are both fixedly connected on the surfaces of the two second rotating rods and are in engagement with each other, the first motor is installed on the right side of the protective shell and its output end movably penetrates through the protective shell, and the output end of the first motor is fixedly connected with the second rotating rod.

[0021] As a preferred scheme of the present application, the surfaces of the second rotating rods and the first rotating rod are both fixedly connected with synchronous wheels, the surfaces of the two synchronous wheels are sleeved with a synchronous belt, and the two synchronous wheels are in transmission connection with the synchronous belt.

[0022] As a preferred scheme of the present application, the heating mechanism comprises a protective sleeve and an electric heating ring, the protective sleeve is sleeved on the surface of the mixing barrel, and the electric heating ring is installed in the protective sleeve.

[0023] As a preferred scheme of the present application, the heating mechanism further comprises handles, mounting plates and screws, the handles are provided in two, the two handles are both fixedly connected on the surface of the protective sleeve, the mounting plates and the screws are both provided in multiple, the mounting plates are all fixedly connected on the protective sleeve, the screws movably penetrate through the mounting plates, and the screws are in threaded connection with the barrel cover.

[0024] As a preferred scheme of the present application, the barrel cover is fixedly connected with two second supporting plates, the second supporting plates are fixedly connected with material cylinders, the material cylinders are in communication with the upper pipes, the bottom of the mixing barrel is fixedly connected with multiple supporting legs, the bottom of the mixing barrel is installed with a discharging pipe, the discharging pipe is installed with a solenoid valve, and the control panel is in electrical connection with the solenoid valve, the liquid inlet valve, the thermometer, the first motor, the second motor and the electric heating ring.

[0025] The preparation method of the rapid cell lysis buffer comprises the following steps:

[0026] S1, raw material preparation:

[0027] The solid raw material is put into the material cylinder, and then the external liquid measuring tank is connected through the liquid inlet pipe;

[0028] S2, put in solid raw material:

[0029] Solid raw materials are fed into the upper pipe through the feed cylinder, and then into the conveying pipe through the upper pipe. At this time, the output end of the first motor is started to rotate, which drives one of the synchronous pulleys to rotate. The synchronous pulleys and synchronous belt drive the first rotating rod to rotate, which in turn drives the spiral conveyor blades to rotate. The rotation of the spiral conveyor blades transports the raw materials in the conveying pipe to the lower pipe, and then into the connecting cylinder through the lower pipe. At the same time, the rotation of the output end of the first motor drives the second rotating rod and the transmission gear to rotate. The rotation of the second rotating rod drives the grinding roller to rotate. Since the two transmission gears are meshed, the two grinding rollers rotate in opposite directions, which facilitates the crushing of solids by the two grinding rollers, thereby facilitating the mixing of solids and liquids.

[0030] S3. Inject the base liquid and mix initially:

[0031] Open the inlet valve and inject approximately 70% of the total volume of deionized water from the liquid metering tank into the mixing tank; start the output end of the second motor to rotate, which in turn drives the third rotor to rotate, which in turn drives multiple stirring rods and stirring blades to rotate, thus facilitating the mixing of solids and liquids. Then, continue stirring to allow the solid raw materials to initially disperse and dissolve in the water, forming a turbid mixture.

[0032] S4. Heat and stir to promote dissolution:

[0033] Activate the heating mechanism on the surface of the mixing tank to heat the mixing tank through the heating coil, and set the temperature of the heating coil to 40-45℃;

[0034] Increasing the stirring speed, under the combined effect of heating and stirring, accelerates the complete dissolution of solid raw materials until the solution becomes clear or homogeneous;

[0035] S5. Add liquid detergent:

[0036] After the solution cools to room temperature, reduce the stirring speed to prevent excessive foaming.

[0037] Open the inlet valves in sequence and slowly add the measured liquid detergent into the mixing tank;

[0038] S6. Volume adjustment and pH adjustment:

[0039] Turn off the heating mechanism, open the liquid inlet valve to add deionized water to the mixing tank until the final target volume is reached, and continue stirring to make the solution uniform.

[0040] S7. Final mixing and discharge:

[0041] After all the ingredients are added, keep stirring at medium speed for 10-15 minutes to ensure that the buffer is completely uniform; turn off the second motor and stop stirring, open the electromagnetic valve, and divide the finished buffer into storage containers.

[0042] Advantages

[0043] Compared with the prior art, the advantages of the present application are:

[0044] (1) In this scheme, the output end of the first motor is started to rotate, one of the synchronous wheels is driven to rotate through the rotation of the first motor, the first rotating rod is driven to rotate through the transmission cooperation of the synchronous wheel and the synchronous belt, the spiral conveying blade is driven to rotate through the rotation of the first rotating rod, and the raw materials in the conveying pipe are transported to the lower pipe through the rotation of the spiral conveying blade, and then are poured into the connecting cylinder through the lower pipe, so that the materials are prevented from being poured into the mixing bucket all at once.

[0045] (2) In this scheme, the output end of the first motor is started to rotate, the second rotating rod is driven to rotate through the rotation of the output end of the first motor, the transmission gear is driven to rotate through the rotation of the second rotating rod, and the grinding roller is driven to rotate through the rotation of the second rotating rod. Since the two transmission gears are engaged, the rotating directions of the two grinding rollers are opposite, so that the two grinding rollers facilitate grinding the solid, thereby facilitating the stirring and mixing of the solid and the liquid.

[0046] (3) In this scheme, the protective sleeve is used to facilitate the installation of the electric heating ring, and the electric heating ring is used to facilitate the heating of the mixing bucket, so as to facilitate the stirring and mixing of the solid raw materials and the liquid raw materials in the mixing bucket.

[0047] (4) In this scheme, the handle is used to facilitate gripping, so as to facilitate lifting away of the protective sleeve, and the mounting plate is used to facilitate cooperation with the screw, so as to facilitate mounting and fixing of the protective sleeve through the threaded connection of the screw and the bucket cover.

[0048] (5) In this scheme, the second supporting plate is used to facilitate fixing of the material cylinder, the material cylinder is used to facilitate placing of the solid raw materials, the supporting leg is used to facilitate supporting of the mixing bucket, the discharge pipe is used to facilitate discharging of the materials, and the electromagnetic valve is used to facilitate control of the discharge of the materials. BRIEF DESCRIPTION OF DRAWINGS

[0049] Figure 1 It is a front view of the present application;

[0050] Figure 2 It is a bottom view of the present application;

[0051] Figure 3 It is an overall exploded view of the present application;

[0052] Figure 4 It is a schematic view of the feeding mechanism of the present application;

[0053] Figure 5 is a cross-sectional view of the delivery tube of the present application;

[0054] Figure 6 is an exploded view of the feed mechanism of the present application;

[0055] Figure 7 is a schematic view of the screw conveyor blade of the present application;

[0056] Figure 8 is a schematic view of the stirring blade of the present application.

[0057] Explanation of reference numerals in the drawings:

[0058] 1, mixing barrel; 2, foot; 3, discharge pipe; 4, electromagnetic valve; 5, barrel cover; 6, liquid inlet pipe; 7, liquid inlet valve; 8, thermometer; 9, connecting cylinder; 10, top cover; 11, control panel; 12, protective shell; 13, first support plate; 14, delivery tube; 15, upper tube; 16, lower tube; 17, second support plate; 18, barrel; 19, first rotating rod; 20, screw conveyor blade; 21, grinding roller; 22, second rotating rod; 23, transmission gear; 24, synchronizing wheel; 25, synchronizing belt; 26, first motor; 27, second motor; 28, third rotating rod; 29, stirring rod; 30, stirring blade; 31, protective sleeve; 32, handle; 33, mounting plate; 34, screw; 35, electric heating ring. DETAILED DESCRIPTION

[0059] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, and not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative labor fall within the protection scope of the present application.

[0060] Embodiment:

[0061] Please refer to Figures 1-8 , a rapid cell lysis buffer, including the following raw materials by weight: Tris-HCl buffer (pH 7.5) 50-70 parts, sodium chloride 8-12 parts, disodium ethylenediaminetetraacetate 1-3 parts, glycerol 15-25 parts, NP-40 detergent 3-7 parts, sodium dodecyl sulfate 0.5-1.5 parts, and protease inhibitor mixture 0.5-1.5 parts.

[0062] The preparation method of the rapid cell lysis buffer, the application discloses a preparation method of a rapid cell lysis buffer, and the device comprises:

[0063] The mixing barrel 1;

[0064] A barrel cover 5 is detachably connected to the mixing barrel 1, a connecting cylinder 9 is installed on the barrel cover 5, a top cover 10 is detachably connected in the connecting cylinder 9, and a control panel 11 is installed on the front end of the connecting cylinder 9;

[0065] A thermometer 8 is installed in the barrel cover 5;

[0066] A second motor 27 is installed at the bottom of the mixing barrel 1, and the output end of the second motor 27 is movably penetrated through the mixing barrel 1, the output end of the second motor 27 is fixedly connected with a third rotating rod 28, the surface of the third rotating rod 28 is fixedly connected with a plurality of stirring rods 29, and the surface of the third rotating rod 28 is fixedly connected with stirring blades 30.

[0067] In the embodiment, the barrel cover 5 is used to seal the mixing barrel 1, the connecting cylinder 9 is used to rotatably connect the grinding roller 21, the top cover 10 is used to communicate with the lower pipe 16, the control panel 11 is electrically connected with the electromagnetic valve 4, the liquid inlet valve 7, the thermometer 8, the first motor 26, the second motor 27 and the electric heating ring 35, so as to control the electromagnetic valve 4, the liquid inlet valve 7, the thermometer 8, the first motor 26, the second motor 27 and the electric heating ring 35 through the control panel 11, the thermometer 8 is used to detect the temperature in the mixing barrel 1, the output end of the second motor 27 is rotated, the third rotating rod 28 is rotated through the rotation of the output end of the second motor 27, the plurality of stirring rods 29 and the stirring blades 30 are rotated through the rotation of the third rotating rod 28, so as to mix the solid and liquid.

[0068] Specifically, the feeding mechanism includes a feeding assembly and a liquid adding assembly, and the feeding assembly and the liquid adding assembly are arranged on the barrel cover 5 and connected with each other.

[0069] The liquid adding assembly includes a liquid inlet pipe 6 and a liquid inlet valve 7, the liquid inlet pipe 6 is installed on the barrel cover 5, and the liquid inlet valve 7 is installed on the surface of the liquid inlet pipe 6.

[0070] In the embodiment, the feeding mechanism includes a feeding assembly and a liquid adding assembly, and the feeding assembly and the liquid adding assembly are arranged on the barrel cover 5 and connected with each other; the liquid inlet pipe 6 is used to connect an external liquid measuring tank, and the liquid inlet valve 7 is used to control the liquid to enter the mixing barrel 1.

[0071] Specifically, the feeding assembly comprises first supporting plates 13, a conveying pipe 14, an upper pipe 15, a lower pipe 16, a first rotating rod 19 and helical conveying blades 20. The first supporting plates 13 are provided in two, and are fixedly connected to the connecting cylinder 9 and the barrel cover 5 respectively. The conveying pipe 14 is fixedly connected to the two first supporting plates 13. The lower pipe 16 is fixedly connected to the bottom of the conveying pipe 14 and is in communication with the top cover 10. The upper pipe 15 is installed on the conveying pipe 14. The first rotating rod 19 is rotatably connected in the conveying pipe 14, and the right end of the first rotating rod 19 is movably penetrated through the right side of the conveying pipe 14. The helical conveying blades 20 are fixedly connected to the surface of the first rotating rod 19.

[0072] In this embodiment, the first supporting plates 13 are used to facilitate the fixation of the conveying pipe 14. The conveying pipe 14 is used to facilitate the connection of the upper pipe 15 and the lower pipe 16, so as to facilitate the feeding through the upper pipe 15 and the discharging through the lower pipe 16. The output end of the first motor 26 is rotated to drive one of the synchronous wheels 24 to rotate through the rotation of the first motor 26. The first rotating rod 19 is driven to rotate through the transmission cooperation of the synchronous wheel 24 and the synchronous belt 25. The helical conveying blades 20 are driven to rotate through the rotation of the first rotating rod 19. The raw materials in the conveying pipe 14 are transported to the lower pipe 16 through the rotation of the helical conveying blades 20, and are poured into the connecting cylinder 9 through the lower pipe 16, so as to prevent the materials from being poured into the mixing barrel 1 at one time.

[0073] Specifically, the feeding assembly further comprises a protective shell 12, grinding rollers 21, second rotating rods 22, transmission gears 23 and a first motor 26. The protective shell 12 is installed on the right side of the connecting cylinder 9. The grinding rollers 21, the second rotating rods 22 and the transmission gears 23 are provided in two. The two grinding rollers 21 are rotatably connected in the connecting cylinder 9. The two second rotating rods 22 are fixedly connected to the right sides of the two grinding rollers 21 respectively, and are movably penetrated through the connecting cylinder 9. The two transmission gears 23 are fixedly connected to the surfaces of the two second rotating rods 22 respectively, and are in engagement. The first motor 26 is installed on the right side of the protective shell 12, and the output end of the first motor 26 is movably penetrated through the protective shell 12. The output end of the first motor 26 is fixedly connected to the second rotating rod 22.

[0074] In this embodiment, the protective shell 12 is used to facilitate the protection of the two transmission gears 23. The output end of the first motor 26 is rotated to drive the second rotating rod 22 to rotate through the rotation of the output end of the first motor 26. The transmission gears 23 are driven to rotate through the rotation of the second rotating rod 22. The grinding rollers 21 are driven to rotate through the rotation of the second rotating rod 22. Since the two transmission gears 23 are in engagement, the rotating directions of the two grinding rollers 21 are opposite, so as to facilitate the crushing of the solid through the two grinding rollers 21, and to facilitate the stirring and mixing of the solid and the liquid.

[0075] Specifically, the surface of the second rotating rod 22 and the first rotating rod 19 is fixedly connected with a synchronous wheel 24, the surfaces of the two synchronous wheels 24 are sleeved with a synchronous belt 25, and the two synchronous wheels 24 are in transmission connection with the synchronous belt 25.

[0076] In the embodiment, the synchronous wheel 24 is used for facilitating transmission cooperation with the synchronous belt 25, and the transmission cooperation of the two synchronous wheels 24 and the synchronous belt 25 facilitates simultaneous rotation of the second rotating rod 22 and the first rotating rod 19.

[0077] Specifically, the heating mechanism comprises a protective sleeve 31 and an electric heating ring 35, the protective sleeve 31 is sleeved on the surface of the mixing barrel 1, and the electric heating ring 35 is installed in the protective sleeve 31.

[0078] In the embodiment, the protective sleeve 31 is used for facilitating installation of the electric heating ring 35, and the electric heating ring 35 is used for facilitating heating of the mixing barrel 1, so as to facilitate stirring and mixing of the solid raw materials and the liquid raw materials in the mixing barrel 1.

[0079] Specifically, the heating mechanism further comprises a handle 32, a mounting plate 33 and a screw 34, the handle 32 is provided as two, the two handles 32 are fixedly connected to the surface of the protective sleeve 31, the mounting plate 33 and the screw 34 are provided as a plurality of, the plurality of mounting plates 33 are fixedly connected to the protective sleeve 31, the plurality of screws 34 are movably penetrated through the plurality of mounting plates 33, and the plurality of screws 34 are threadedly connected to the barrel cover 5.

[0080] In the embodiment, the handle 32 is used for facilitating gripping, so as to facilitate lifting of the protective sleeve 31, and the mounting plate 33 is used for facilitating cooperation with the screw 34, and the protective sleeve 31 is facilitated to be installed and fixed through the threaded connection of the screw 34 and the barrel cover 5.

[0081] Specifically, the barrel cover 5 is fixedly connected with two second supporting plates 17, the two second supporting plates 17 are fixedly connected with a material cylinder 18, the material cylinder 18 is in communication with the upper pipe 15, the bottom of the mixing barrel 1 is fixedly connected with a plurality of supporting legs 2, the bottom of the mixing barrel 1 is provided with a discharging pipe 3, the discharging pipe 3 is provided with an electromagnetic valve 4, and the control panel 11 is in electrical connection with the electromagnetic valve 4, the liquid inlet valve 7, the thermometer 8, the first motor 26, the second motor 27 and the electric heating ring 35.

[0082] In the embodiment, the second supporting plate 17 is used for facilitating fixation of the material cylinder 18, the material cylinder 18 is used for facilitating placement of the solid raw materials, the supporting leg 2 is used for facilitating support of the mixing barrel 1, the discharging pipe 3 is used for facilitating discharging, and the electromagnetic valve 4 is used for facilitating control of discharging of the materials.

[0083] The preparation method of the rapid cell lysis buffer comprises the following steps:

[0084] S1, raw material preparation:

[0085] The solid raw material is put into the barrel 18, and then the external liquid metering tank is connected through the liquid inlet pipe 6;

[0086] S2, put in solid raw materials:

[0087] The solid raw material is put into the upper pipe 15 through the barrel 18, and then put into the conveying pipe 14 through the upper pipe 15. At this time, the output end of the first motor 26 is started to rotate, one of the synchronous wheels 24 is driven to rotate through the rotation of the first motor 26, the first rotating rod 19 is driven to rotate through the transmission cooperation of the synchronous wheel 24 and the synchronous belt 25, the spiral conveying blade 20 is driven to rotate through the rotation of the first rotating rod 19, the raw material in the conveying pipe 14 is transported to the lower pipe 16 through the rotation of the spiral conveying blade 20, and then put into the connecting barrel 9 through the lower pipe 16; At the same time, the output end of the first motor 26 drives the second rotating rod 22 and the transmission gear 23 to rotate, and the rotation of the second rotating rod 22 drives the grinding roller 21 to rotate. Since the two transmission gears 23 are engaged, the rotation directions of the two grinding rollers 21 are opposite, so that the solid can be easily crushed by the two grinding rollers 21, thereby facilitating the stirring and mixing of the solid and the liquid

[0088] S3, inject the base liquid and mix preliminarily:

[0089] Open the liquid inlet valve 7, inject about 70% of the total volume of deionized water into the mixing barrel 1 from the liquid metering tank; the output end of the second motor 27 is started to rotate, the third rotating rod 28 is driven to rotate through the rotation of the output end of the second motor 27, and the plurality of stirring rods 29 and stirring blades 30 are driven to rotate through the rotation of the third rotating rod 28, thereby facilitating the stirring and mixing of the solid and the liquid. Then keep stirring to preliminarily disperse and dissolve the solid raw material in water to form a turbid mixed solution;

[0090] S4, heating and stirring to promote dissolution:

[0091] Start the heating mechanism on the surface of the mixing barrel 1, heat the mixing barrel 1 by the electric heating ring 35, and set the temperature of the electric heating ring 35 to 40-45℃;

[0092] Increase the stirring speed, and under the combined action of heating and stirring, accelerate the complete dissolution of the solid raw material until the solution becomes clear or uniform;

[0093] S5, add liquid detergent:

[0094] After the solution cools to room temperature, reduce the stirring speed to prevent excessive foam;

[0095] Open the liquid inlet valve 7 in sequence, and slowly add the metered liquid detergent into the mixing barrel 1;

[0096] S6, constant volume and pH adjustment:

[0097] Close the heating mechanism, open the liquid inlet valve 7 to add deionized water to the mixing barrel 1 until the final target volume, and continue stirring to make the solution uniform;

[0098] S7, final mixing and discharging:

[0099] After all the ingredients are added, keep the stirring speed at medium speed for 10-15 minutes to ensure that the buffer is completely uniform; turn off the second motor 27 to stop stirring, open the electromagnetic valve 4, and then divide the finished buffer into storage containers.

[0100] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can make equivalent replacements or changes within the technical range disclosed by the present application according to the technical solution and improvement concept of the present application, which should be covered within the protection scope of the present application.

Claims

1. A rapid cell lysis buffer, characterized in that, The ingredients include the following parts by weight: 50-70 parts Tris-HCl buffer (pH 7.5), 8-12 parts sodium chloride, 1-3 parts disodium EDTA, 15-25 parts glycerol, 3-7 parts NP-40 detergent, 0.5-1.5 parts sodium dodecyl sulfate, and 0.5-1.5 parts protease inhibitor mixture.

2. A method for preparing a rapid cell lysis buffer, characterized in that, The device includes: Mixing bucket (1); A bucket lid (5) is detachably connected to a mixing bucket (1). A connecting cylinder (9) is installed on the bucket lid (5). A top cover (10) is detachably connected inside the connecting cylinder (9). A control panel (11) is installed at the front end of the connecting cylinder (9). Thermometer (8), which is installed inside the barrel lid (5); The second motor (27) is installed at the bottom of the mixing tank (1), and the output end of the second motor (27) moves through the mixing tank (1). The output end of the second motor (27) is fixedly connected to a third rotating rod (28). Multiple stirring rods (29) are fixedly connected to the surface of the third rotating rod (28), and stirring blades (30) are fixedly connected to the surface of the third rotating rod (28). The feeding mechanism is disposed on the mixing tank (1) and is connected to the tank cover (5); A heating mechanism is provided on the outside of the mixing tank (1) and is connected to the mixing tank (1).

3. The method for preparing the rapid cell lysis buffer according to claim 2, characterized in that: The feeding mechanism includes a feeding component and a liquid adding component, both of which are mounted on the barrel cover (5) and are connected to each other. The liquid addition assembly includes an inlet pipe (6) and an inlet valve (7). The inlet pipe (6) is installed on the bucket cover (5), and the inlet valve (7) is installed on the surface of the inlet pipe (6).

4. The method for preparing the rapid cell lysis buffer according to claim 3, characterized in that: The feeding assembly includes a first support plate (13), a conveying pipe (14), an upper pipe (15), a lower pipe (16), a first rotating rod (19), and a spiral conveying blade (20). There are two first support plates (13), which are respectively fixedly connected to the connecting cylinder (9) and the barrel cover (5). The conveying pipe (14) is fixedly connected to the two first support plates (13). The lower pipe (16) is fixedly connected to the bottom of the conveying pipe (14) and is connected to the top cover (10). The upper pipe (15) is installed on the conveying pipe (14). The first rotating rod (19) is rotatably connected inside the conveying pipe (14), and the right end of the first rotating rod (19) moves through the right side of the conveying pipe (14). The spiral conveying blade (20) is fixedly connected to the surface of the first rotating rod (19).

5. The method for preparing the rapid cell lysis buffer according to claim 4, characterized in that: The feeding assembly also includes a protective shell (12), a grinding roller (21), a second rotating rod (22), a transmission gear (23), and a first motor (26). The protective shell (12) is installed on the right side of the connecting cylinder (9). The grinding roller (21), the second rotating rod (22), and the transmission gear (23) are all configured in pairs. The two grinding rollers (21) are rotatably connected inside the connecting cylinder (9). The two second rotating rods (22) are respectively fixedly connected to the right side of the two grinding rollers (21), and the two second rotating rods (22) move through the connecting cylinder (9). The two transmission gears (23) are respectively fixedly connected to the surface of the two second rotating rods (22), and the two transmission gears (23) mesh with each other. The first motor (26) is installed on the right side of the protective shell (12), and the output end of the first motor (26) moves through the protective shell (12). The output end of the first motor (26) is fixedly connected to the second rotating rod (22).

6. The method for preparing the rapid cell lysis buffer according to claim 5, characterized in that: Both the second rotating rod (22) and the first rotating rod (19) are fixedly connected to a synchronous pulley (24), and a synchronous belt (25) is sleeved on the surface of the two synchronous pulleys (24), and the two synchronous pulleys (24) are connected to the synchronous belt (25) for transmission.

7. The method for preparing the rapid cell lysis buffer according to claim 6, characterized in that: The heating mechanism includes a protective sleeve (31) and an electric heating coil (35). The protective sleeve (31) is fitted onto the surface of the mixing tank (1), and the electric heating coil (35) is installed inside the protective sleeve (31).

8. The method for preparing the rapid cell lysis buffer according to claim 7, characterized in that: The heating mechanism also includes a handle (32), a mounting plate (33), and screws (34). There are two handles (32), both of which are fixedly connected to the surface of the protective sleeve (31). There are multiple mounting plates (33) and screws (34). Multiple mounting plates (33) are fixedly connected to the protective sleeve (31). Multiple screws (34) move through multiple mounting plates (33) and are threaded onto the bucket lid (5).

9. The method for preparing the rapid cell lysis buffer according to claim 8, characterized in that: Two second support plates (17) are fixedly connected to the lid (5), and a material cylinder (18) is fixedly connected to the two second support plates (17). The material cylinder (18) is connected to the upper pipe (15). Multiple support legs (2) are fixedly connected to the bottom of the mixing tank (1). A discharge pipe (3) is installed at the bottom of the mixing tank (1). A solenoid valve (4) is installed on the discharge pipe (3). The control panel (11) is electrically connected to the solenoid valve (4), the liquid inlet valve (7), the thermometer (8), the first motor (26), the second motor (27), and the heating coil (35).

10. A method for preparing rapid cell lysis buffer, characterized in that, The method includes the following steps: S1. Raw material preparation: Solid raw materials are fed into the feed cylinder (18) and then connected to an external liquid metering tank through the liquid inlet pipe (6); S2, Input solid raw materials: Solid raw materials are fed into the upper pipe (15) through the feed cylinder (18), and then into the conveying pipe (14) through the upper pipe (15). At this time, the output end of the first motor (26) is started to rotate. The rotation of the first motor (26) drives one of the synchronous pulleys (24) to rotate. Through the transmission cooperation of the synchronous pulley (24) and the synchronous belt (25), the first rotating rod (19) is driven to rotate. The rotation of the first rotating rod (19) drives the spiral conveying blade (20) to rotate. The rotation of the spiral conveying blade (20) moves the conveying pipe... The raw materials in (14) are transported to the lower pipe (16) and fed into the connecting cylinder (9) through the lower pipe (16); at the same time, the output end of the first motor (26) rotates to drive the second rotating rod (22) and the transmission gear (23) to rotate, and the rotation of the second rotating rod (22) drives the grinding roller (21) to rotate. Since the two transmission gears (23) mesh, the two grinding rollers (21) rotate in opposite directions, which makes it easier to crush the solid through the two grinding rollers (21), thus making it easier to mix the solid and liquid. S3. Inject the base liquid and mix initially: Open the inlet valve (7) and inject about 70% of the total volume of deionized water from the liquid metering tank into the mixing tank (1); start the output end of the second motor (27) to rotate, and drive the third rotating rod (28) to rotate through the output end of the second motor (27), and drive multiple stirring rods (29) and stirring blades (30) to rotate through the rotation of the third rotating rod (28), so as to facilitate the mixing of solid and liquid. Then keep stirring so that the solid raw materials are initially dispersed and dissolved in water to form a turbid mixture. S4. Heat and stir to promote dissolution: Start the heating mechanism on the surface of the mixing tank (1) and heat the mixing tank (1) through the heating coil (35), and set the temperature of the heating coil (35) to 40-45℃; Increasing the stirring speed, under the combined effect of heating and stirring, accelerates the complete dissolution of solid raw materials until the solution becomes clear or homogeneous; S5. Add liquid detergent: After the solution cools to room temperature, reduce the stirring speed to prevent excessive foaming. Open the inlet valve (7) in sequence and slowly add the metered liquid detergent into the mixing tank (1); S6. Volume adjustment and pH adjustment: Turn off the heating mechanism, open the liquid inlet valve (7) to add deionized water to the mixing tank (1) until the final target volume is reached, and continue to stir to make the solution uniform; S7. Final mixing and discharge: After all ingredients have been added, keep stirring at medium speed for 10-15 minutes to ensure the buffer solution is completely homogeneous; turn off the second motor (27), stop stirring, open the solenoid valve (4), and dispense the finished buffer solution into storage containers.

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