A soothing anti-aging composition containing saffron ferment and a method of preparing the same

Abstract

The present application relates to a kind of saffron ferment-containing soothing anti-aging composition and its preparation method, specifically to utilize microbial inoculant to saffron pollen fermentation, and be supplemented with peony flower extract, ginseng petal extract and be obtained by mixing fermentation product, the composition of the present application is not only high safety, and can efficiently remove free radical, effectively anti-aging and wrinkle removal, with wide market application prospect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to a soothing and anti-aging composition containing saffron ferment and its preparation method. Background Technology

[0002] Fermentation is a process that utilizes the growth of microbial cells or biocatalytic reaction systems to obtain the microbial cells themselves or their metabolites. By selecting specific microbial strains and controlling the fermentation process, the industrial-scale processing and manufacturing of target products can be achieved. Plant fermentation technology refers to a class of technologies that utilize microorganisms to slowly ferment natural plant raw materials, releasing the active substances in the plants. These substances are then further modified, altered, or decomposed by microbial enzymes to obtain substances that are beneficial for skin absorption and enhance skincare efficacy. With the deepening of research and development in the cosmetics field, the use of microbial fermentation to prepare cosmetics has become a research hotspot in my country's cosmetics market.

[0003] Saffron (scientific name: *Crocus sativus* L.) is a perennial herbaceous plant belonging to the genus *Crocus* in the family Iridaceae. "Saffron" is the original plant name, while "crocus" is the medicinal name in pharmacopoeias. Crocus is the dried stigma of the saffron flower, also known as "Tibetan saffron." Saffron has been used for over three thousand years as a flavoring, aromatherapy, dye, and medicine. The medicinal part of saffron is the dried stigma, measured by weight. Saffron is one of the world's most expensive spices, characterized by its sweet taste and distinctive hay-like odor. From a traditional Chinese medicine perspective, saffron is sweet and neutral in nature; it enters the heart and liver meridians; it has the effects of promoting blood circulation, removing blood stasis, cooling the blood and detoxifying, relieving depression and calming the mind. Clinical trials have shown that saffron has the potential to become an anti-cancer and anti-aging agent. In addition to its medicinal uses, saffron has long been used as a dye for textiles.

[0004] However, the yield of saffron stigmas is extremely low, accounting for only 7.4% of the total flower weight, and the remaining discarded parts result in a significant waste of resources. Therefore, the development and utilization of the non-medicinal parts of saffron is a research hotspot. Research on the non-medicinal parts of saffron mainly focuses on petals, stamens, pollen, and corms, with petals being the most studied. These non-medicinal parts contain flavonoids, phenolic acids, monoterpenes, glycosides, alkaloids, anthraquinones, and saponins, with flavonoids and phenolic acids being the main components. Saffron flower extract is primarily used as a skin conditioning agent in cosmetics and skincare products. It is relatively safe and can provide antioxidant, skin brightening, anti-inflammatory, soothing, and moisturizing effects. Its natural active ingredients can neutralize free radicals, inhibit melanin production, alleviate skin sensitivity, and enhance barrier function, making it suitable for various skin types. Saffron is rich in carotenoids and crocin, which can effectively neutralize free radicals, reduce oxidative stress, and slow down collagen loss, thereby improving skin laxity and fine lines. In addition, saffron extract can block tyrosinase activity, reduce melanin deposition, and improve dullness and yellowing. The anti-inflammatory components in saffron (such as crocin) can reduce the release of inflammatory factors in the skin and alleviate the burning sensation of sensitive skin. Meanwhile, polysaccharides form a protective film on the skin surface to prevent moisture loss and improve dryness and peeling.

[0005] In addition, the peony (Paeonia suffruticosa), a plant belonging to the Ranunculaceae family and the Paeonia genus, has seen research and application in skincare in recent years, mainly focusing on peony root bark, seeds, and flowers. Peony root bark (also known as peony bark or danpi) is a traditional Chinese medicine. Extensive research has found it to be rich in various active substances such as paeonol, paeonol glycosides, and paeoniflorin, possessing excellent antioxidant and anti-aging effects. Peony seed oil, produced from peony seeds, has excellent antioxidant capacity and can be used as a base oil or active ingredient in skincare products. Furthermore, ginseng is a renowned and precious medicinal herb, with a history of using ginseng for health preservation and disease treatment spanning over 4000 years. Ginseng is also a highly valued beauty product, historically known as the "elixir for wrinkled skin." Ginseng contains ginsenosides, essential oils, ginseng acid, vitamins, etc., which promote skin metabolism and delay aging. Its mechanism of action is to inhibit melanin production by melanocytes and reduce melanin granules. Related studies have demonstrated that ginsenosides have whitening effects.

[0006] Existing technology CN120478250A discloses an essence with anti-wrinkle and skin-repairing effects, including plant extracts such as saffron extract and Leuconostoc mellitus / radish root fermentation product filtrate, which can significantly enhance the anti-wrinkle, repairing, and brightening effects of the essence, thereby providing consumers with comprehensive skin care and helping to effectively improve the overall quality of the skin. Existing technology CN119454546A discloses a firming and wrinkle-reducing composition containing saffron extract, etc., used in combination with peptides, suitable for use in skin care products such as eye creams, gentle and non-irritating, with good stability. These existing technologies have not developed cosmetics that utilize microbial fermentation of plant ingredients and prepare them into vesicle compositions. However, with the continuous development and iteration of technology, emerging traditional Chinese medicine exovesicle skin care products have significant advantages in terms of bioactivity, safety, and transdermal absorption. In addition, compared with exosomes derived from animal cells, traditional Chinese medicine exovesicles have a wider range of applications in the skin care field and are more conducive to large-scale production and industrialization. Based on existing technologies, this invention focuses on researching the preparation of cosmetics from fermented plant extracts delivered via vesicles, and their efficacy. Summary of the Invention

[0007] This invention is based on the aforementioned known prior art and aims to provide a skin care composition for treating or preventing changes in skin such as photoaging and fine lines caused by aging, and to provide a basic cosmetic and manufacturing method based on the composition.

[0008] Specifically, the present invention provides a soothing and anti-aging composition containing saffron fermentation product, comprising saffron pollen vesicles, wherein the pollen vesicles contain products obtained by fermenting saffron pollen using red yeast.

[0009] Furthermore, the method for preparing the pollen vesicles includes the following steps:

[0010] Step S1: Mix saffron pollen with distilled water at a mass ratio of 1:1, put the mixture into a sealed bag, vacuum it, and place it in an ultra-high pressure sterilizer. Control the pressure at 500-800 MPa and the temperature at 45-65℃ for 15 minutes. Add 1.2-1.5% agar, heat it to 50-60℃, cool it, and set it aside to obtain the saffron pollen matrix.

[0011] Step S2: Inoculate the activated red yeast into the saffron pollen substrate obtained in S1, and ferment at 26-28℃ for 48-72 hours to obtain the primary fermentation product.

[0012] Step S3: Add the primary fermentation product obtained in step S2 to the *Lactobacillus plantarum* culture medium at a ratio of 1:2 to 1:3, and inoculate with activated *Lactobacillus plantarum*. Incubate at 30-37℃ for 48-72 hours, collect the culture product, sterilize at 120℃, and centrifuge at 8000-12000g for 30-40 minutes to obtain the high molecular weight precipitate and secondary fermentation product.

[0013] Step S4: The polymer precipitate obtained in step S3 is ultrasonically broken up to lyse the bacterial cell fragments. After filtration, the filtrate is collected, and secondary fermentation products are added. After microfluidic homogenization, the mixture is freeze-dried to obtain pollen vesicles.

[0014] Furthermore, step S4 also includes adding ginseng extract and peony flower extract after collecting the filtrate.

[0015] Furthermore, the red yeast inoculation concentration is 4-6 × 10⁻⁶. 6 cfu / g.

[0016] Furthermore, the inoculation concentration of *Lactobacillus plantarum* is 4-6 × 10⁻⁶. 6 cfu / g.

[0017] Furthermore, another aspect of the present invention provides the use of a composition in the preparation of cosmetics, characterized in that the composition is the aforementioned soothing and anti-aging composition containing saffron ferment.

[0018] Furthermore, another aspect of the present invention provides a cosmetic product, characterized in that the cosmetic product comprises the aforementioned composition.

[0019] Furthermore, the cosmetic also contains butylene glycol.

[0020] Furthermore, the cosmetic product is selected from one or more dosage forms in the group consisting of lotion, toner, cream, facial mask, facial cleanser, body lotion, foundation, serum, massage cream, and hand cream.

[0021] Furthermore, the cosmetic product has the effect of increasing the content of type I collagen in the skin.

[0022] In this invention, the culture medium for microbial fermentation is common knowledge in the art, such as a nitrogen-limiting culture medium, the formulation of which may be 50.0 g / L glucose, 1.5 g / L yeast extract, 0.3 g / L (NH4)2SO4, 2.0 g / L KH2PO4, 1.5 g / L MgSO4·7H2O, 0.1 g / L CaCl2, 0.04 g / L FeCl3, 0.01 g / L ZnSO4, 0.002 g / L CuSO4, 0.005 g / L MnSO4, 0.02 mg / L biotin, and 2 g / L citric acid.

[0023] In this invention, the red yeast is selected from strain Rhodotorula mucilaginosa MHT01 with accession number CGMCC.31295, or strain with accession number CGMCC AS 2.1817. Lactobacillus plantarum: accession number CGMCC.32813.

[0024] In this invention, the indicators for detecting the effects of the composition are based on common knowledge in the art. For example, collagen is a functional protein, accounting for 30% of all proteins in the body. In human skin, approximately 75% of the protein is collagen, maintaining skin firmness. However, with age, the amount of collagen in the skin gradually decreases, leading to atrophy, thinning, fragility, poor pigmentation, and delayed wound healing, resulting in loss of skin elasticity and firmness, and the formation of wrinkles. Skin fragility is partly attributed to changes in laminonoid granules and downregulation of collagen (type I) expression at the dermal-epidermal junction.

[0025] Furthermore, bacterial lipopolysaccharide (LPS) binds to macrophage surface antigen recognition receptors, inducing macrophages to secrete various cytokines such as TNF-α. TNF-α can activate three signaling pathways: caspase, JNK, and the transcription factor NF-κB, thereby achieving its biological functions of cytotoxicity, antiviral activity, immunomodulation, and apoptosis, making it an important inflammatory factor. When the skin is exposed to ultraviolet radiation, various inflammatory factors (mainly IL-6, TNF-α, and IL-8 mediated by NF-κB) and inflammatory mediators (NO synthesized by iNOS and PGE2 mediated by EP2) play key roles in the occurrence of acute and chronic inflammation. Therefore, these factors are also commonly used as indicators for evaluation.

[0026] The beneficial effects of this invention include:

[0027] (1) The pollen vesicles prepared by this invention have high safety, can efficiently scavenge free radicals, and effectively resist aging.

[0028] (2) The composition of the present invention has the effect of enhancing type I collagen in the skin, and also provides good support for maintaining the microecology of the skin surface and improving the skin barrier.

[0029] (3) The composition of the present invention can effectively soothe the skin and eliminate skin inflammation, especially the inflammation of the dermis, providing an ideal raw material for the development of soothing cosmetics. Attached Figure Description

[0030] Figure 1 The composition of the present invention is shown in the histological examination results for improving photoaging: model group.

[0031] Figure 2 Histological examination results of the composition of the present invention in improving photoaging: medium dose group. Detailed Implementation

[0032] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention are within the scope of protection of the present invention.

[0033] Example 1

[0034] Example 1: The method for preparing pollen vesicles in this invention includes the following steps:

[0035] Step S1: Mix saffron pollen with distilled water at a mass ratio of 1:1, put the mixture into a sealed bag, vacuum it, and place it in an ultra-high pressure sterilizer. Control the pressure at 650 MPa and the temperature at 55°C for 15 minutes. Add 1.5% agar, heat to 55°C, cool, and set aside to obtain saffron pollen matrix.

[0036] Step S2, the activated red yeast (accession number: CGMCC.31295, 5×10⁻⁶) 6 (cfu / g) was inoculated into the saffron pollen matrix obtained in S1 and fermented at 28℃ for 60 h to obtain the primary fermentation product;

[0037] Step S3: Add the primary fermentation product obtained in step S2 to the *Lactobacillus plantarum* culture medium at a ratio of 1:2, and inoculate with activated *Lactobacillus plantarum* (accession number: CGMCC.32813, 5.5 × 10⁻⁶). 6 (cfu / g), cultured at 37℃ for 60h, the culture product was collected, sterilized at 120℃, and centrifuged at 10000g for 35min to obtain the polymer precipitate and secondary fermentation product;

[0038] Step S4: The polymer precipitate obtained in step S3 is sonicated (200W, 20s breakage, 20s interval) to lyse the bacterial cell fragments. After filtration (Millipore 0.22-micron filter membrane), the filtrate is collected, and secondary fermentation products, ginseng extract, and peony flower extract (added in a weight ratio of 3:1:1) are added. After homogenization by microfluidic jet (8000psi, 5 cycles), the mixture is freeze-dried to obtain pollen vesicles.

[0039] Comparative Example 1-1

[0040] The red yeast was selected from strain Rhodotorula mucilaginosa MHT01, with accession number CGMCC.31295. Activation methods included streaking yeast extract onto glucose agar to obtain pure colonies, picking single colonies and activating them to the logarithmic development phase in yeast extract glucose agar; inoculating the logarithmic-phase red yeast seed culture at a 5% inoculum into nitrogen-limited medium; fermentation conditions: controlled temperature 28℃, dissolved oxygen 80%, fermentation time 72h; at the end of fermentation, adjusting pH to 3.5 with 3% hydrochloric acid solution to create an acidic environment, enhancing cell disruption efficiency and oil release; raising the fermenter temperature to 70℃ and maintaining it for 30min, then cooling to 30℃; after fermentation, inactivating the yeast at 70℃ for 30min; homogenizing the fermentation broth under low-temperature microfluidic pressure of 3000psi and temperature of 4℃ for 45min; centrifuging at 8000rpm for 30min to remove cell debris; filtering and collecting the filtrate; and freeze-drying for storage.

[0041] Comparative Examples 1-2

[0042] Lactobacillus plantarum: Preservation number CGMCC.32813; Strain activation: Take one loopful of slant culture and inoculate it into MRS liquid medium. Incubate at 30℃ for 24 hours to activate the strain. Inoculation: Inoculate the activated strain into fresh MRS liquid medium at an inoculation rate of 4% (volume fraction). Anaerobic culture: Incubate the inoculated culture solution anaerobically at 30℃ for 24 hours. After the incubation period, inactivate the strain at 70℃ for 30 min. Homogenize the culture solution under low-temperature microfluidic pressure of 3000 psi and temperature of 4℃ for 45 min. Centrifuge at 8000 rpm for 30 min to remove bacterial fragments. Filter and collect the filtrate, then freeze-dry for storage.

[0043] Comparative Example 2-1

[0044] Referring to Example 1, saffron pollen was mixed with distilled water at a mass ratio of 1:1, placed in a sealed bag, vacuumed, and placed in an ultra-high pressure sterilizer. The pressure was controlled at 650 MPa and the temperature at 55°C for 15 minutes for sterilization. Then, the Lactobacillus plantarum culture medium was added according to the method described in steps 3-4 of Example 1 for fermentation and preparation of pollen vesicles.

[0045] Comparative Example 2-2

[0046] Referring to Example 1, saffron pollen was mixed with distilled water at a mass ratio of 1:1, placed in a sealed bag, vacuum-sealed, and placed in an ultra-high pressure sterilizer. The pressure was controlled at 650 MPa and the temperature at 55°C for 15 minutes. Agar was added, and the mixture was heated to 55°C, then cooled and set aside to obtain saffron pollen matrix. Activated red yeast was inoculated into the saffron pollen matrix obtained in S1, fermented at 28°C for 60 hours, sterilized at 120°C, and centrifuged at 10000g for 35 minutes. Then, it was prepared according to the method in step S4 of Example 1 to obtain pollen vesicles.

[0047] Comparative Examples 2-3

[0048] Polymer precipitate and secondary fermentation product were prepared according to steps S1-S3 of Example 1; ginseng extract and peony flower extract (in a weight ratio of 3:1:1) were added to the secondary fermentation product, homogenized by microfluidic jet (8000psi, 5 cycles), and then freeze-dried to obtain pollen vesicles.

[0049] Comparative Example 3

[0050] Polymer precipitate and secondary fermentation product were prepared according to steps S1-S3 of Example 1. The polymer precipitate obtained in step S3 was subjected to ultrasonication (200W, 20s disruption, 20s interval) to lyse the bacterial cell fragments. After filtration (Millipore 0.22-micron filter membrane), the filtrate was collected, the secondary fermentation product was added, and the mixture was homogenized by microfluidic jet (8000psi, 5 cycles) and then freeze-dried to obtain pollen vesicles.

[0051] Free radical scavenging performance determination

[0052] The method is derived from "T / SHRH 006-2018 Cosmetics - Free Radical Scavenging Test Method". The principle is that DPPH is a stable, long-lived free radical. Its ethanol solution is deep purple and exhibits strong absorption near 517 nm. When a free radical scavenger is present, the light absorption of the DPPH ethanol solution weakens due to the pairing of single electrons with it. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, thus allowing evaluation of the sample's ability to scavenge free radicals, i.e., the strength of its antioxidant activity. The measurement procedure is common knowledge in the field, including but not limited to: using 1.5 mL centrifuge tubes to set up the test sample tube (T), the test sample background (T0), the DPPH tube (C), and the solvent background (C0), with three parallel tubes for each sample and three parallel tubes for the DPPH tube. Add 0.1 mL of the same concentration (3% after safety evaluation) of sample solution to each of the test sample tubes and the test sample background. Add supplementary water to all tubes to bring the total to 0.3 mL, and mix well. Add 0.1 mL of DPPH ethanol solution to the sample tubes and DPPH tubes. Replace the sample background and solvent background with 95% ethanol. Gently shake and let stand at room temperature for 5 min. Transfer 200 μL of each reaction solution into a 96-well plate and measure the absorbance at 517 nm using a microplate reader. Calculate the DPPH free radical scavenging rate using the following formula:

[0053] DPPH free radical scavenging rate (%) = [1 - (T - T0) / (C - CO)] × 100%

[0054] Wherein, T represents the absorbance of the sample tube, i.e., the absorbance of the solution after the sample reacts with DPPH; T0 represents the background absorbance of the sample; C represents the average of three absorbance readings of the DPPH tube, i.e., the absorbance of the DPPH solution without sample; and CO represents the background absorbance of the solvent. Statistical analysis is expressed as mean ± SD, and statistical analysis was performed using SPSS software (compared with the blank control group). The detection results are shown in Table 1.

[0055] Table 1. Test results of free radical scavenging performance

[0056] Test group DPPH free radical scavenging rate (%) p-value Test results Comparative Example 1-1 5.1±0.11 - - Comparative Examples 1-2 8.3±0.20 - - Comparative Example 2-1 16.5±0.16 <0.01 Significant Comparative Example 2-2 18.7±0.15 <0.01 Significant Comparative Examples 2-3 14.4±0.23 <0.01 Significant Comparative Example 3 20.6±0.14 <0.01 Significant Example 1 23.4±0.04 <0.01 Significant

[0057] As shown in Table 1, compared with Comparative Examples 1-1 or 1-2, the DPPH free radical scavenging rate of each group of the test samples increased significantly at a concentration of 3%, indicating that the samples have good antioxidant effects and the pollen vesicle composition has a good synergistic effect. Further analysis showed that the scavenging rate of Example 1 was also significantly different from that of Comparative Examples 2 (2-1, 2-2, 2-3) (p<0.05), and the scavenging rate was the best under the formulation conditions of Example 1. In addition, the addition of ginseng extract and peony flower extract had a synergistic effect on the efficacy of the composition and could further improve its antioxidant capacity.

[0058] Example 2

[0059] This embodiment simulates the evaluation of anti-aging and anti-wrinkle effects of a sample by detecting the upregulation rate of type I collagen content in human dermal fibroblasts after UVA radiation. Specific procedures are standard knowledge in the field, including but not limited to: seeding human dermal fibroblasts into 96-well plates (2×10⁻⁶ wells). 4 Cells were incubated at 37°C and 5% CO2 for 24 hours. Cell groups are shown in Table 2 below. After incubation, the culture medium was removed, and the cells were washed twice with D-Hanks ink. Then, the sample group and the model control group were exposed to UVA irradiation for 8 hours at 9 J / cm². 2 After modeling, the normal control group and the model control group were replaced with fresh culture medium. Fresh culture medium containing the sample from Example 1 (final concentration 3%) was added to the sample group. Three biological replicates were performed, and the cells were incubated at 37°C and 5% CO2 for 24 hours. The cell supernatant from each well was collected, and the type I collagen content was detected by ELISA. The upregulation rate was calculated using the following formula:

[0060] Upregulation rate (%) = (Tsample group - Tmodel control group) / Tmodel control group × 100%

[0061] Where T represents the average type I collagen content, and the statistical results are expressed as mean ± SD. Statistical analysis was performed using SPSS software. The independent samples t-test was used for statistical analysis between the two groups. The statistical results were defined with α = 0.05 as the test limit, and p < 0.05 was considered statistically significant. The results are shown in Table 3, where NC represents the model control group.

[0062] Table 2 Cell grouping

[0063]

[0064] Table 3 Measurement Results

[0065]

[0066] The test results showed that the type I collagen content in the model control group was significantly lower than that in the normal control group, indicating that the secondary conditions for testing type I collagen content were effective. The type I collagen content in the sample group was significantly higher than that in the model control group, indicating that the pollen vesicle composition of the present invention has significant anti-aging and anti-wrinkle functions.

[0067] Example 3

[0068] Twenty-five healthy male SPF-grade SD rats were randomly divided into four groups: control group, model group, low-dose group, medium-dose group, and high-dose group, with five rats in each group. After one week of acclimatization, the rats in the model group and the low, medium, and high-dose groups were irradiated with UV light to establish a skin photoaging animal model. The shaving SD rats were placed at a distance of 30 cm from a UV light source and irradiated every two days. The irradiation time was 30 min in weeks 1-2, 40 min in weeks 3-4, 50 min in weeks 5-6, and 60 min in weeks 7-8. The UVA intensity was approximately 148.50 J / cm², and the UVB intensity was approximately 21.38 J / cm². Starting from week 5 of modeling, the low, medium, and high dose groups were given samples at concentrations of 1%, 5%, and 10%, respectively (the composition of Example 1 was mixed with butylene glycol by mass fraction), applied to the back of each rat at a rate of 0.1 g / site. The control and model groups were given an equal volume of physiological saline mixed with butylene glycol. This treatment was continued for 4 weeks. After the experiment, the rats were euthanized with CO2, and photoaged skin tissue from the backs of SD rats was quickly harvested and rinsed in pre-cooled physiological saline at 4°C to remove excess blood. After blotting with absorbent paper, the tissue was weighed. Some skin tissue was fixed in 4% neutral formaldehyde solution. The tissue underwent routine paraffin embedding, sectioning, dewaxing, hydration, hematoxylin staining, dehydration, clearing, and mounting with neutral resin. Histopathological changes were observed under a high-power microscope (HE×200). The results are shown in the appendix. Figure 1 - Appendix Figure 2 0.5g of skin tissue was added to 1mL of 4℃ physiological saline solution and homogenized into a paste. The paste was centrifuged at 5000rpm for 20min, and the supernatant was collected. This supernatant was used to determine the levels of NO, PGE2, TNF-α, and IL-6 in the skin tissue. The detection method was ELISA, and the procedure was strictly followed according to the kit instructions (Wuhan Yipu Biotechnology Co., Ltd.). The results are shown in Table 4.

[0069] Table 4. Results of determination of soothing and anti-aging factors in skin tissue (mean±SD)

[0070] Group NO (μM) TNF-α (pg / mL) PGE2 (pg / mL) IL-6 (pg / mL) control group 2.91±0.095 1551±10.9 532±61.6 20.7±5.3 Model group 14.7±0.061 55553±2257.1 2020±129.2 9802.2±741.2 low-dose group 11.3±0.095 44631±606.5 1404±150.1 7720.7±753.4 medium dose group 8.8±0.036 3119±327.9 1139±103.9 5794.6±477.6 High-dose group 7.5±0.047 2854±352.4 1051±144.0 5421.5±435.7

[0071] Histological section results showed that in the control group rats, the stratum corneum, epidermis, and dermis were clearly defined and well-defined. In the model group rats, the epidermis was thickened, the stratum corneum was thickened and accompanied by hyperkeratosis, and inflammatory cell infiltration was observed in the dermis. In contrast, the epidermal thickening and stratum corneum thickening were alleviated in the low, medium, and high dose treatment groups, the stratum corneum thickening was improved, and the inflammatory cell infiltration in the dermis was reduced (the histological results of the medium dose group are shown in the attached figure). The results of soothing factor assays showed that after administration of the composition of this invention, the content of soothing factors in the skin tissue was significantly reduced. In the low dose group, the reduction rate of TNF-α content was 20%, the reduction rate of PGE2 content was 30%, the reduction rate of NO content was significantly reduced by 23%, and the reduction rate of IL-6 was 26%.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A soothing anti-aging composition containing saffron ferment, characterized in that, The composition contains safflower pollen vesicles, the pollen vesicles comprising a product obtained by step-by-step fermentation of safflower pollen using red yeast and plant lactobacillus, and a preparation method of the pollen vesicles comprising the following steps: Step S1, mixing safflower pollen with distilled water at a mass ratio of 1:1, loading into a sealed bag, vacuumizing, placing into an autoclave, controlling the pressure at 500-800 MPa, the temperature at 45-65℃, sterilizing for 15 minutes, adding agar 1.2-1.5%, heating to 50-60℃, then cooling, standby, obtaining a safflower pollen substrate; Step S2, inoculating the activated red yeast into the safflower pollen substrate obtained in S1, fermenting at 26-28℃ for 48-72h, obtaining a primary fermentation product; Step S3, adding the primary fermentation product obtained in step S2 into the plant lactobacillus culture medium at a ratio of 1:2-1:3, inoculating the activated plant lactobacillus, culturing at 30-37℃ for 48-72h, collecting the culture product, sterilizing at 120℃, centrifuging at 8000-12000g for 30-40min, obtaining a high molecular precipitate and a secondary fermentation product; Step S4, ultrasonic crushing the high molecular precipitate obtained in step S3, lysing the bacterial fragments, collecting the filtrate after filtration, adding ginseng extract and peony extract, adding the secondary fermentation product, homogenizing by microflow, freeze-drying, obtaining pollen vesicles.

2. The composition of claim 1, wherein, The Rhodotorula inoculum concentration is 4-6 x 10 6 cfu / g.

3. The composition of claim 2, wherein, The inoculation concentration of the plant Lactobacillus is 4-6 x 10 6 cfu / g.

4. Use of a composition for the manufacture of a cosmetic product, characterized in that, The composition is a soothing anti-aging composition containing safflower fermentation product according to claim 1.

5. A cosmetic product, characterized by, The cosmetic product comprises the composition according to claim 1.

6. The cosmetic product according to claim 5, characterized in that, The cosmetic product further comprises butylene glycol.

7. The cosmetic product according to claim 5, characterized in that, The cosmetic product is selected from any one of the dosage forms of emulsion, toner, cream, mask, foundation, serum. The composition is a soothing anti-aging composition containing safflower fermentation product according to claim 1. The cosmetic product comprises the composition according to claim 1. The cosmetic product further comprises butylene glycol. The cosmetic product is selected from any one of the dosage forms of emulsion, toner, cream, mask, foundation, serum.

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