A skin care composition resistant to environmental pollution

Abstract

This invention relates to the field of cosmetics, specifically to a skincare composition for combating environmental pollution. The composition comprises rice germ extract and a mixture of rice extracts, a mixture of *Chlorella vulgaris* extract and *Lithocarpus stolonifera* extract, *Microcystis globosum* extract, and a mixture of olive fruit water and olive fruit extract as main active ingredients. Through a rational ratio of these components, the resulting composition can isolate air pollutants, inhibit their adhesion to sebum, prevent them from penetrating the skin, and effectively reduce sebum secretion by decreasing the size and number of facial pores. It also increases the skin's tolerance to irritants, enhances antioxidant capacity, and reduces the likelihood of inflammation caused by sebum peroxidation. Furthermore, it improves cell viability to resist air pollutants.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to an environmental pollution resistant skin care composition. BACKGROUND

[0002] The main components of air pollutants are particulate matter, ozone, nitrogen dioxide, sulfur dioxide and carbon monoxide, among which particulate matter has attracted widespread attention due to its serious harm to health. The definition of particulate matter is based on its aerodynamic diameter to distinguish, such as particulate matter (diameter ≤ 10 μm), fine particulate matter (diameter ≤ 2.5 μm) and other classifications. Studies have shown that the smaller the particle size of particulate matter, the greater the harm. Many epidemiological studies have shown that an increase in PM 2.5 concentration is closely related to the incidence of cardiovascular and respiratory diseases.

[0003] In addition to the damage to the cardiovascular and respiratory systems, the organ most susceptible to PM 2.5 is the skin, because PM 2.5 is derived from smoke, exhaust from vehicles and other emissions from production or life, and is composed of elemental carbon, organic compounds such as polycyclic aromatic hydrocarbons, various inorganic salts, heavy metal elements and mixed biological substances. When the skin is exposed to polluted air for a long time, it is easy to cause skin damage.

[0004] The reasons for the penetration of air pollutants into the skin are as follows: according to the research of Kyung Eun Kim, 1) hair follicles are generated in the stratum corneum and open into pores in the epidermis, so hair follicles not only block the function of the stratum corneum as a skin barrier, but also provide a penetration path for pollutants, allowing particles of different diameters to penetrate the skin through pores of different sizes; 2) the opening of the pore is also prone to accumulation of chemical substances (Life Sciences 2016, Vol. 152, Pages 126-134); 3) the pore is the outlet for sebum and sweat and other secretions, and sebum mixes with sweat on the surface of the skin, covering the surface and playing an important role in protecting the skin from external stimuli. However, sebum is prone to adhere to particulate matter and chemical substances (G. Peterson, S. Rapaka, N. Koski et al., Int. J. Cosmet. Sci., 2017, 39, 351-354), increasing the chances of penetration of pollutants. Therefore, if the size and number of pores can be reduced to inhibit sebum secretion and prevent the adhesion of pollutants on the sebum, the harm of pollutants to the skin and the occurrence of diseases can be greatly reduced.

[0005] From the emergence of anti-pollution cosmetics, to the recent years anti-pollution has become a trend in the field of skin care, the main problem solving path is as follows: (1) remove pollutants in a clean way; (2) physical barrier, reduce pollutant adsorption; (3) traditional Chinese medicine extraction composition to maintain skin vitality; (4) repel positively charged atmospheric dust and complex harmful heavy metals; (5) anti-acid liquid, anti-ultraviolet.

[0006] But there is still a fundamental problem in existing research that is often overlooked, that is, facial oil is easy to combine with particulate matter, when the number of pores on the skin is too much or the pore size is too large, not only easy to make pollutants invade the inside of the skin, but also easy to secrete too much sebum, adhere to a larger amount of air pollutants, which will be difficult to remove by cleaning. SUMMARY

[0007] In view of the fact that human skin is easily damaged under environmental pollution in the prior art, pollutants invade the skin, damage the skin barrier, cause the levels of vitamin E and squalene to decrease, collagen and elastin to decompose, and aggravate skin problems such as skin dehydration, pigmentation, photoaging, excessive sebum secretion, inflammation and skin sensitivity, the present application provides an anti-environmental pollution skin care composition, which comprises a mixture of rice germ extract and rice extract, a mixture of chondrus crispus extract and lithothamnium albus extract, nannochloropsis oculata extract, a mixture of olea europaea fruit water and olea europaea fruit extract and the like as main functional ingredients, through reasonable proportioning of various components, the obtained composition can isolate air pollutants, inhibit their adhesion to sebum, prevent their invasion into the inside of the skin, reduce the size and number of facial pores to effectively reduce sebum secretion; increase the tolerance of the skin to irritants, improve the antioxidant capacity, and reduce the probability of inflammation caused by sebum peroxidation. Improve cell survival capacity to resist air pollutants.

[0008] The technical scheme of the present application comprises:

[0009] An anti-environmental pollution skin care composition, which comprises, by weight percentage, 1-8% of macadamia integrifolia seed oil, 2-8% of simmondsia chinensis seed oil, 1-6% of squalane, 2-5% of persea gratissima butter, 0.1-2% of bisabolol, 0.1-2% of a mixture of rice germ extract and rice extract, 0.1-4% of a mixture of chondrus crispus extract and lithothamnium albus extract, 0.1-3% of nannochloropsis oculata extract, 0.1-4% of a mixture of olea europaea fruit water and olea europaea fruit extract, 0.1-2.5% of bioflavonoids, 0.1-0.5% of sodium hyaluronate, 6-49% of adjuvant, and water in excess.

[0010] The adjuvant includes at least an emulsifier allowed in cosmetics; as further preferred, it also includes a preservative allowed in cosmetics, and other adjuvants allowed in cosmetics such as emollients, skin conditioning agents, thickening agents, etc. can also be added.

[0011] Preferably, the emulsifier of the adjuvant is a cuticle lipid, and the content of the cuticle lipid in the skin care composition is between 2-12% by weight;

[0012] As further preferred, the preservative is ethylhexylglycerin / glyceryl caprylate (a mixture of ethylhexylglycerin and glyceryl caprylate), the mass ratio of ethylhexylglycerin to glyceryl caprylate in ethylhexylglycerin / glyceryl caprylate is 1:1, the emollient is diethylhexyl carbonate, the skin conditioning agent is 1,2-pentanediol and arginine, and the thickening agent is polyacryloyldimethyltaurate sodium;

[0013] The content of ethylhexylglycerin / glyceryl caprylate in the skin care composition is 0.5-1.0% by weight, the content of diethylhexyl carbonate is 2.5-5%, the content of 1,2-pentanediol is 1-5%, the content of arginine is 0.01-0.05%, and the content of polyacryloyldimethyltaurate sodium is 0.2-0.6%.

[0014] Preferably, the rice germ extract and rice extract mixture is specifically BLUE Oléoactif ® .

[0015] Preferably, the Chondrus crispus extract and Lithothamnium extract mixture is specifically OLIGOGELINE™ SPE.

[0016] Preferably, the Nannochloropsis oculata extract is specifically PEPHA ® -TIGHT CB.

[0017] The Nannochloropsis oculata extract forms a film on the skin surface, making the skin have an immediate firming effect, and can isolate pollutants from penetrating deep into the skin, inhibiting their stimulation of the skin.

[0018] The olive fruit water and olive fruit extract mixture is specifically ITALINE ® O.

[0019] The rice germ extract and rice extract mixture and the olive fruit water and olive fruit extract mixture are both natural ingredients, and are mild in nature. The two components complement each other in function and have a synergistic effect. The combination of the two can significantly improve the efficiency of free radical scavenging, and can simultaneously efficiently scavenge water-soluble free radicals and lipid-soluble free radicals.

[0020] The combined use of keratinocyte-like lipids and a mixture of *Chlorella vulgaris* extract and *Lithocarpus stolonifera* extract forms a physical barrier between pollutants and sebum, preventing excessive pollutant absorption by sebum and subsequent penetration into the skin, thus avoiding inflammation and damage. The addition of bioflavonoids reduces the size and number of facial pores, thereby decreasing sebum secretion and reducing the likelihood of pollutants penetrating the skin through pores.

[0021] This invention further provides a method for preparing the above-mentioned anti-environmental pollution skincare composition, comprising the following steps:

[0022] (1) Add excipient A and water as phase A raw material to a vacuum emulsifying pot and dissolve them at 80±3℃ under high speed and low speed double stirring in the vacuum emulsifying pot for at least 20 min to obtain phase A;

[0023] (2) Sodium hyaluronate and water are used as phase B raw materials and stirred in a mixing pot at 800±50 rpm for at least 20 min to be completely dispersed. Then they are added to the vacuum emulsification pot of step (1) and dissolved at 80±3℃ under high speed and low speed double stirring in the vacuum emulsification pot for at least 20 min to obtain phase B.

[0024] (3) In another mixing pot, the whole-leaved macadamia seed oil, auxiliary material B, jojoba seed oil, squalane, shea butter and bisabolol are used as raw materials for phase C. Heat to 80±3℃ and stir until fully dissolved. Stir at 250±50 rpm for at least 20 minutes to obtain phase C.

[0025] (4) Inject the C phase in the mixing pot in step (3) into the vacuum emulsifying pot in step (2) for emulsification;

[0026] (5) Perform emulsification in the main pot of the vacuum emulsifying pot;

[0027] (6) Stir and cool the vacuum emulsifying pot to 30°C;

[0028] (7) Add rice germ extract and rice extract mixture, wrinkled hornweed extract and spurious algae extract mixture, eye-like vein microphobic algae extract, olive fruit water and olive fruit extract mixture, and bioflavonoids as D phase raw materials to the vacuum emulsifying pot of step (6) and stir.

[0029] (8) Degass and filter, and the material is ready for discharge.

[0030] The excipient A is a skin conditioning agent and / or a thickener; the excipient B is one or more of a skin moisturizer, preservative, and emulsifier.

[0031] Preferably, the stirring speeds of the vacuum emulsifying pot in steps (1) and (2) are: 500±50 rpm for high-speed stirring and 60±5 rpm for low-speed stirring.

[0032] The mass ratio of the water added in step (1) to the water added in step (2) is 2.0 to 3.0:1;

[0033] The conditions for emulsification in the vacuum emulsifying pot in step (4) are: temperature: 80±3℃, low speed: 60±5 rpm, high speed: 1500±50 rpm, time: 10~15 minutes;

[0034] The conditions for emulsification in the main pot in step (5) are: temperature: 80±3℃, low speed: 60±5 rpm, high speed: 2000±50 rpm, and time: 20-30 minutes;

[0035] In step (6), cooling to 30°C is specifically achieved by using a vacuum emulsifying pot with a low speed of 60±5 rpm, a high speed of 0 rpm, and a time of 30 to 50 minutes. Once the temperature reaches 30°C, the process is stopped.

[0036] In step (7), the raw material of phase D is added to the vacuum emulsification pot and stirred as follows: low speed 60±5 rpm, high speed 0 rpm, temperature 25~30℃ for 20 minutes.

[0037] A and B are aqueous phases, and C is an oil phase. They need to be processed into homogeneous phases before they can be mixed and emulsified at a specific temperature. The D phase raw material is a mixture of functional ingredients. To avoid deactivation caused by high temperature, the D phase raw material must be added to the mixture obtained by mixing the emulsified A, B, and C phase raw materials after emulsification is completed and the mixture is cooled down.

[0038] If the ingredients are not added in the order described above, the following situation will occur:

[0039] 1. Incomplete emulsification will result in oil-water separation and will not produce a uniform emulsion state;

[0040] 2. Emulsions prepared by direct mixing have poor stability;

[0041] 3. Due to the above circumstances, a protective film cannot be formed on the outer layer of the skin to block pollutants, and the active ingredients cannot penetrate into the skin to exert all the effects mentioned in this patent.

[0042] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0043] 1) The rice germ extract and rice extract mixture, and the olive fruit water and olive fruit extract mixture, have complementary functions and synergistic effects:

[0044] According to reports (Research Progress on Free Radicals in the Human Body. Chinese Journal of Epidemiology, 2016, 37(8):1175-1182), various free radicals exist in the human body, among which oxygen free radicals are the most widely studied, including superoxide anion free radicals (O2•). - The free radicals include hydroxyl radicals (OH•), hydroperoxide radicals (HO2•), lipid peroxide radicals (LOO•), and nitric oxide radicals (NO•), among which nitric oxide radicals include nitric oxide cations, nitric oxide anions, and peroxynitrite ions (ONOO). - Highly reactive nitrogen-containing substances such as hydrogen peroxide (H2O2) and singlet oxygen are also known as reactive nitrogen species (RNS). 1 O2, hypochlorous acid (HClO), and other oxygen-containing free radicals are collectively referred to as reactive oxygen species (ROS).

[0045] The main component of the rice germ extract and rice extract mixture is vitamin E. Vitamin E is a fat-soluble component and has a good neutralizing effect on lipid peroxidation free radicals, alkyl free radicals and hydrogen peroxide free radicals, but its scavenging effect on water-soluble free radicals is relatively weak (Role of vitamin E as a lipid-soluble peroxylradical scavenger: in vitro and in vivo evidence. Free Radical Biology and Medicine. 2014,66:3-12).

[0046] The mixture of olive fruit water and olive fruit extract contains oleuropein and hydroxytyrosol, both of which are water-soluble components. Therefore, it is effective against hydrogen peroxide, hydroxyl radicals (OH•), and superoxide anion radicals (O2•). - ), nitric oxide radicals (NO•), peroxynitrite (ONOO) - Its ability to scavenge water-soluble free radicals is significantly higher than that of vitamin E.

[0047] The significant difference in the overall antioxidant capacity (ORAC) scores of the two components is also evident. These two components are functionally complementary and synergistic; their combined use significantly enhances the scavenging efficiency of free radicals, effectively eliminating both water-soluble and lipid-soluble free radicals simultaneously.

[0048] 2) The mixture of cuticle-like lipids and *Chlorella vulgaris* extract and *Lithocarpus spurii* extract has a synergistic effect:

[0049] Using the above components together creates a physical barrier between pollutants and sebum, preventing excessive pollutant absorption by sebum and subsequent invasion of the skin, which can cause inflammation and damage. The addition of bioflavonoids reduces the size and number of facial pores, thereby decreasing sebum secretion and reducing the likelihood of pollutants penetrating the skin through the pores.

[0050] The main components of this stratum corneum-like lipid compound are plant-derived amphiphilic molecules. Carefully balanced dosages are used to form a stable, biomimetic lipid-based layered gel system. Components include low-water-soluble glyceryl monostearate, palmitic acid, and stearic acid; smaller hydroxyl groups such as behenol, cetyl alcohol, myristol, and lauryl alcohol; and amphiphilic lecithin. At physiological temperatures, it can mimic the ordered layered structure of stratum corneum lipids, which is crucial for building a strong skin barrier and hydration. The main component of the mixture of *Chlorella vulgaris* extract and *Lithocarpus septemlobus* extract is carrageenan. The synergistic effects of the two are as follows:

[0051] a. The keratinocyte-like lipids can penetrate deep into the stratum corneum to replenish lipids, while the mixture of Carrageenan extract and Lithops extract helps to form a film on the skin surface, reducing moisture evaporation. This dual mechanism can achieve better moisturizing and water-locking effects.

[0052] b. Creatinoid lipids help repair damaged sebum films. The film-forming properties of the mixture of Carrageenan extract and Lithops extract provide an additional physical protective layer, which is especially effective for sensitive skin.

[0053] c. Creatinoid lipids can alter the structure of the stratum corneum and increase its permeability. The mixture of *Chlorella vulgaris* extract and *Lithocarpus stolonifera* extract forms a protective film, reducing the volatilization of active ingredients and aiding in ingredient retention and absorption. Simultaneously, the mixture of *Chlorella vulgaris* extract and *Lithocarpus stolonifera* extract also has a penetration-enhancing effect.

[0054] 3) The extract of Micrococcus oxyphylla contains polysaccharides that help retain moisture and can work synergistically with keratinoid lipids to moisturize. It contains arachidonic acid (EPA), a polyunsaturated fatty acid with five double bonds. It is an ω-3 fatty acid that can repair cell membranes and work with keratinoid lipids to repair the skin barrier and strengthen protection against environmental stress. Attached Figure Description

[0055] Figure 1 A schematic diagram of the area before contamination of the skincare composition of Example 1, after applying artificial sebum;

[0056] Figure 2 A schematic diagram of the contaminated area treated with the skincare composition of Example 1 after applying artificial sebum;

[0057] Figure 3 A schematic diagram of the area before contamination in the blank control group of Example 1, where artificial sebum was applied;

[0058] Figure 4 A schematic diagram of the contaminated area in Example 1 (blank control group) after application of artificial sebum;

[0059] Figure 5 A schematic diagram of the pre-contamination area of ​​the skin care composition group (Comparative Example 1) after applying artificial sebum;

[0060] Figure 6 A schematic diagram of the contaminated area of ​​the Comparative Example 1 skincare composition group after applying artificial sebum;

[0061] Figure 7 A schematic diagram of the area before contamination in the control group (Example 1) after applying artificial sebum;

[0062] Figure 8 A schematic diagram of the contaminated areas after the application of artificial sebum, compared to the blank control group in Example 1.

[0063] Figure 9 A schematic diagram of the pre-contamination area of ​​the skin care composition group 2 (Comparative Example 2) after applying artificial sebum;

[0064] Figure 10 A schematic diagram of the contaminated area of ​​the Comparative Example 2 skincare composition group after applying artificial sebum;

[0065] Figure 11 A schematic diagram of the area before contamination in the control group (Example 2) after applying artificial sebum;

[0066] Figure 12 A schematic diagram of the contaminated areas after the application of artificial sebum, compared to the blank control group in Example 2.

[0067] Figure 13 Example image of subject 018 (VISIA6 Standard light source mode photo), where: BL - before test, W2 - two weeks after use, W4 - four weeks after use;

[0068] Figure 14 Example image of subject 026 (VISIA6 Standard light source mode photo), where: BL - before test, W2 - two weeks after use, W4 - four weeks after use;

[0069] Figure 15 Example image of subject 025 (VISIA6 Standard light source mode photo), where: BL - before test, W2 - two weeks after use, W4 - four weeks after use;

[0070] Figure 16Example image of subject 032 (VISIA6 Standard light source mode photo), where: BL - before test, W2 - two weeks after use, W4 - four weeks after use. Detailed Implementation

[0071] The following detailed embodiments further illustrate the above-described content of the present invention, but should not be construed as limiting the scope of the invention to the following examples. All technologies implemented based on the above-described content of the present invention fall within the scope of the present invention. Unless otherwise specified, conventional techniques are used in the following embodiments.

[0072] In the following examples, the rice germ extract and rice extract mixture specifically refers to BLUE Oléoactif ® (HALLSTAR, France); the mixture of *Chlorella vulgaris* extract and *Lithocarpus spurius* extract is specifically OLIGOGELINE™ SPE (SEPPIC, France); the *Microcystis glomeratus* extract is specifically PEPPIC. ® -TIGHT CB (DSM); The mixture of olive fruit water and olive fruit extract is specifically ITALINE. ® O (AKOTT, Italy), stratum corneum lipids were purchased from Ashland's ProLipid. TM 141 lamellar gel, the bioflavonoids were purchased from Ashland's elixiance™ biofunctional, and the CAS number for shea butter is 194043-92-0.

[0073] In the following ethylhexylglycerol / glycerocaprylate, the mass ratio of ethylhexylglycerol to glycerocaprylate is 1:1.

[0074] The ingredient compositions of the skin care compositions of Examples 1-5 and Comparative Examples 1-9 are shown in Tables 1 and 2:

[0075] Table 1. Weight ratio of each component raw material in the antifouling compositions of Examples 1, 4, 5 and Comparative Examples 1, 2, 8, 9 (the sum of the weight percentages of each raw material is 100%).

[0076]

[0077] Table 2. Weight ratio of each component raw material in the antifouling compositions of Examples 2, 3 and Comparative Examples 3-7 (the sum of the weight percentages of each raw material is 100%).

[0078]

[0079] The skin care compositions of Examples 1-5 and Comparative Examples 1-9 were all obtained by the following preparation method. The vacuum emulsifying pot used was a dual-speed stirring device commonly used in the art (such as a dual-stirring scraper reactor). Its high-speed (emulsifying head) and low-speed (scraping and stirring blades) were alternately stacked, and the bottom was a homogenizing head for high-speed stirring. A coaxial dual-speed stirring device could be used, or a non-coaxial dual-speed stirring device could be used (such as a high-speed emulsifying head set on the central axis, and a low-speed scraping and stirring blade fixed to the rotatable inner wall and rotating with the inner wall).

[0080] The specific steps are as follows:

[0081] (1) Add phase A raw material to the vacuum emulsifying pot (main pot), heat and stir evenly, and dissolve by stirring at high speed (emulsifying head speed) and low speed (scraping and stirring blades) (both stirring are carried out simultaneously). Temperature: 80±3℃, high speed: 500±50rpm, low speed: 60±5rpm, time: at least 20 minutes to obtain phase A;

[0082] (2) After the B phase raw material is completely dispersed (stirring pot, stirring speed 800±50 rpm, time at least 20 minutes), it is added to the main pot of the vacuum emulsifying pot described in step (1) so that the B phase raw material is added to the A phase. Heat and stir evenly, and dissolve by stirring at high speed (emulsifying head speed) and low speed (scraping stirring blade) in the emulsifying pot (double stirring is carried out simultaneously). Temperature: 80±3℃, high speed: 500±50 rpm, low speed: 60±5 rpm, time at least 20 minutes to obtain the B phase;

[0083] (3) Add the C phase raw material into the mixing pot (the mixing pot for the C phase and the B phase are different), heat to 80±3℃ and stir to dissolve fully, stirring at 250±50 rpm for at least 20 minutes to obtain the C phase;

[0084] (4) Inject the C phase in the mixing pot in step (3) into the main pot (i.e., the vacuum emulsifying pot containing the B phase in step (2)) for emulsification. Temperature: 80±3℃, low speed: 60±5 rpm, high speed: 1500±50 rpm, time: 10-15 minutes.

[0085] (5) Perform emulsification in the main pot at a temperature of 80±3℃, a low speed of 60±5 rpm, a high speed of 2000±50 rpm, and a time of 20-30 minutes.

[0086] (6) Stir and cool to 30°C, low speed: 60±5 rpm, high speed: 0 rpm, time: 30-50 minutes, stop when 30°C is reached;

[0087] (7) Add the D phase raw material to the main pot, low speed: 60±5 rpm, high speed: 0 rpm, time: 20 minutes, temperature 25~30℃;

[0088] (8) Defoaming and filtration, the skin care composition is obtained immediately after discharge, and then sampling and testing and material transfer can be carried out.

[0089] A and B are aqueous phases, and C is an oil phase. They need to be processed into homogeneous phases before they can be mixed and emulsified at a specific temperature. The D phase raw material is a mixture of functional ingredients. To avoid deactivation caused by high temperature, the D phase raw material must be added to the mixture obtained by mixing the emulsified A, B, and C phase raw materials after emulsification is completed and the mixture is cooled down.

[0090] Experimental Example 1: Pollutant Adhesion Test

[0091] Pollution conditions were simulated using a pollution simulation device. Images of human skin adsorbing simulants in the test sample area and the blank control area were taken using Skin PHOTOMAX. Data analysis software (IMAGE PRO PLUS) was used to determine the area percentage of deposited contaminants. This experiment was commissioned to Shanghai Microspection Testing Technology Group Co., Ltd. to analyze the amount of simulants adsorbed on human skin.

[0092] Testing process:

[0093] Subjects cleaned the test area on the inside of their arms with water. After cleaning, they sat quietly for 30 minutes in a temperature and humidity controlled room (temperature: 22±2℃; humidity: 50%RH±10%RH). The front of the subject's arm was divided into 8 areas, each 2×2cm. 2 Artificial sebum was applied to four test areas to simulate normal skin oil production; the other four areas were left untreated. From each of the four areas treated with and untreated areas, three areas were randomly selected for treatment, and the remaining area was left untreated (blank control group).

[0094] The test was commissioned to Shanghai Microspection Testing Technology Group Co., Ltd., with the search result being "anti-pollution effect study". The method was roughly as follows: approximately 10g of pollution simulant (5000-mesh carbon powder) was placed in a pollution simulation device (WPE-GX0074). An arm was placed inside the device, and the pollution simulation was activated for 2 minutes (2 minutes for the fan, 1 minute for the pump, with both the fan and pump running simultaneously). A PM2.5 detector was used to monitor the pollutants (5000-mesh carbon powder, no other pollutants, concentration controlled at 1000±50 μg / m³) in the pollution simulation device chamber during the test. 3After turning off the pollution simulation device, the arm was rotated three times in the device to shake off excess unadsorbed particles. Then, the arm was photographed using a dermoscope PHOTOMAX on the test area.

[0095] The test results are shown in Table 3:

[0096] Table 3. Results of pollutant adhesion test

[0097]

[0098] Relative change rate (%) = [(Percentage of contaminant area on the sample side using the sample - Percentage of contaminant area on the sample side not using the sample) / Percentage of contaminant area on the sample side not using the sample] × 100%.

[0099] Photos of the above-mentioned Example 1, Comparative Example 1, and Comparative Example 2 before and after pollution simulation ( Figures 1-12 The blank control cases in each example underwent the same treatment: artificial sebum was applied but no skincare composition was applied. Figures 1-12 As shown in Table 3 and Figures 1-12 It can be seen that applying artificial sebum increases the adhesion of pollutants by about two times. After using Example 1, Comparative Example 1, and Comparative Example 2, the adhesion of pollutants was significantly reduced. In particular, Example 1, which uses a mixture of keratinoid lipids, Carrageenan extract, and Lithops extract, is more effective than Comparative Example 1 and Comparative Example 2, which use the two components alone.

[0100] Experiment Example 2: Facial Pore Size and Count Test

[0101] The skin analysis system VISIA6 (CANFIELD, USA) was used to evaluate changes in skin physiological indicators such as pore area ratio and number of facial pores before and after product use, thus assessing the product's effectiveness. Tests were conducted before product use, two weeks after use, and four weeks after use. The results are shown in Table 7 below.

[0102] Test subjects: 1) Healthy Chinese subjects aged 18-60, both male and female; 2) Pore size grade 1-3 (graded according to L'Oréal Skin Aging Atlas Volume 2: Asian Type [M]); 3) Forehead sebum level exceeding 120 μg / cm² within 8 hours. 2 ;

[0103] The skincare compositions of Example 2 and Comparative Example 3 were used for testing. The subject parameters of Example 2 and Comparative Example 3 are shown in Tables 4 and 5, respectively, and the test procedures are shown in Table 6.

[0104] Table 4 Subject parameters tested using Example 2

[0105]

[0106] Table 5. Subject parameters using Comparative Example 3 test

[0107]

[0108] Table 6 Test Procedure

[0109]

[0110] The evaluation criteria are two indicators: the percentage of pore area and the number of pores, measured by instruments. The instrument models used and the judgment methods are as follows:

[0111] (1) Percentage of pore area

[0112] Measuring instrument: VISIA6 skin analysis system (CANFIELD, USA);

[0113] Measurement requirements: Acquire images once (front 0°, left 33°, right 33°), and analyze the images once;

[0114] Parameter explanation: The lower the value, the smaller the proportion of pore area in the test area. Unit: %.

[0115] (2) Number of pores

[0116] Testing instrument: VISIA6 skin analysis system (CANFIELD, USA);

[0117] Measurement requirements: Acquire images once (front 0°, left 33°, right 33°), and analyze the images once;

[0118] Parameter explanation: The lower the value, the fewer the number of facial pores. Unit: pores.

[0119] The test results are shown in Table 7:

[0120] Table 7. Effects of the skincare compositions of Example 2 and Comparative Example 3 on facial cleanliness and pore size.

[0121]

[0122] Based on the above results, it can be shown that the skin care composition of Example 2 can significantly reduce the proportion of pore area and the number of pores after use, and can improve facial cleanliness.

[0123] Four facial images of subjects who used the skincare composition product of Example 2 for four weeks were selected. The results showed that the pore area ratio and number of pores on the subjects' faces were significantly improved. The subjects were numbered 018, 025, 026, and 032. Figures 13-16 As shown.

[0124] From Table 7 and Figures 13-16 It can be seen that after using the skin care compositions of Example 2 and Comparative Example 3 for 4 weeks, the improvement of various physiological indicators is very significant. In particular, Example 2 plays a very important role in reducing the proportion of pore area and the number of pores, and can effectively prevent pollutants in the air from penetrating into the skin.

[0125] The comparison of the test results of Example 2 and Comparative Example 3 shows that bioflavonoids are very effective ingredients for reducing the area ratio of pores and reducing the number of pores.

[0126] Experimental Example 3: Skin Tolerance Test for Irritants

[0127] The skin tolerance test was conducted by Beijing Yiweishi Testing Technology Co., Ltd. - China National Light Industry Council Cosmetic Efficacy Evaluation and Testing Center (Beijing). Subjects cleansed their faces morning and evening, first applying their usual serum, then pressing out 0.3±0.05 grams of the test product and applying it evenly to their faces. Throughout the test period, subjects were prohibited from prolonged sun exposure, outdoor sports, travel, etc., and were not allowed to use cosmetics or medications with similar effects to the product. Furthermore, subjects were not allowed to change their daily skincare routines.

[0128] Before using the product and at 2 and 4 weeks of product use, a nasolabial fold lactic acid stinging test was performed. Trained professional researchers inquired about the subjects' subjective symptoms, scored them on a 4-point scale, and recorded the results. The results are shown in Table 8. The evaluation criteria are as follows:

[0129] 0 points, no stinging, burning, or itching;

[0130] 1 point, mild stinging, burning, and itching;

[0131] 2 points, I clearly felt stinging, burning, and itching;

[0132] 3 points. I felt a very strong stinging, burning, itching, and even unbearable pain.

[0133] The degree of improvement is calculated as follows: [(average of the 4-point assessment values ​​before use - average of the 4-point assessment values ​​after use) / (average of the 4-point assessment values ​​before use)] × 100%. The test results are shown in Table 8.

[0134] Table 8. Improvement in lactic acid stinging score of any side of the nasolabial fold

[0135]

[0136] The test results in Table 8 show that the four components—stratum corneum lipids, a mixture of olive fruit water and olive fruit extract, a mixture of rice germ extract and rice extract, and bisabolol—work synergistically to increase the skin's tolerance to irritants. Reducing any of the aforementioned components significantly decreases the degree of skin improvement.

[0137] Experimental Example 4: Antioxidant (ORAC) Capacity

[0138] ORAC uses azo compound azobisisobutyramidine hydrochloride (AAPH) as the peroxide free source, sodium fluorescein as the fluorescent indicator, and the vitamin E water-soluble analog Trolox as the quantitative standard. Analysis was performed using a fluorescence microplate analyzer (QWP-SHACDDTW-WIF-1094 ORAC Oxidative Radical Absorption Capacity Test Specification). In the presence of antioxidants, the antioxidants can compete with sodium fluorescein for a reaction, thereby inhibiting the resulting fluorescence changes and slowing the rate of fluorescence decay. The degree of inhibition can evaluate the antioxidant capacity of the sample. The test results are shown in Table 9.

[0139] The procedure for testing the ability to absorb free radicals is briefly described below:

[0140] (1) First, prepare phosphate buffer (pH=7.4, 0.2 M), then use the above phosphate buffer to prepare luciferin working solution, AAPH solution and Trolox working solution (prepared using phosphate buffer at 6.7×10⁻⁶ M). -2 Prepare a 118 mM AAPH solution using phosphate buffer. Prepare Trolox working solutions at concentrations of 50, 60, 70, 80, 100, and 200 μg / mL using phosphate buffer.

[0141] (2) Add various samples and solutions to the 96-well plate (add each sample in sequence (add 20 μL of the test sample solution and Trolox working solution to each well, the test sample solution is one of the 25% and 50% (m / V) test sample solutions prepared with phosphate buffer from Example 4, Comparative Example 8 and Comparative Example 9), add 20 μL of fluorescein working solution and 140 μL of AAPH solution with a concentration of 118 mM to each well), and continuously measure the fluorescence intensity at an excitation wavelength of 485 nm and an emission wavelength of 535 nm. The entire system is kept at 37°C, and the fluorescence intensity is measured every 5 min. The measurement time is generally set until the fluorescence decays to the baseline.

[0142] (3) The experiment requires two controls: a FL fluorescence natural decay control without the addition of free radicals (-AAPH, i.e., no AAPH solution added) and a free radical action control without the presence of antioxidants (+AAPH, i.e., AAPH solution added but no sample solution added). (Standard procedure for ORAC experiment: FL fluorescence natural decay control without the addition of free radicals (-AAPH) and free radical action control without the presence of antioxidants (+AAPH); use Trolox as a standard, plot a standard curve, and express the ORAC value of the sample in terms of Trolox equivalents); the antioxidant capacity of the sample is directly related to the area of ​​the delayed portion of the fluorescence decay curve under the action of free radicals (netAUC).

[0143] Table 9 Results of Oxidation Free Radical Absorption Capacity Test

[0144]

[0145] As shown in Table 9, the ORAC values ​​of Comparative Example 8 (rice germ extract and rice extract mixture) and Comparative Example 9 (olive fruit water and olive fruit extract mixture) used alone were both below 130 at the sample concentration (50%, m / V). However, when these two components were used together, the ORAC value increased to 140, proving that the two components (rice germ extract and rice extract mixture and olive fruit water and olive fruit extract mixture) had a synergistic effect on improving the antioxidant capacity of the product.

[0146] Experiment 5: Cell Viability

[0147] Testing system: human fibroblasts.

[0148] Test method:

[0149] 1) Cell seeding: at 5 × 10 4 Cells were seeded at a density of cells / well into 48-well plates and incubated overnight in an incubator (37°C, 5% CO2).

[0150] 2) Prepare the culture medium: 99.5% pure water + 0.5% hydrolyzed milk protein (Haibo Biotechnology, HB8851, http: / / www.haibobiol.com / products / show2489745.html), stir well;

[0151] Prepare a maltose medium with a mass concentration of 3%: Add 3 grams of maltose to 97 grams of the medium and stir well;

[0152] Sample culture media with mass concentrations of 0.0125%, 0.0065%, and 0.0031% were prepared: 0.0125, 0.0065, and 0.0031 grams of the anti-environmental pollution skin care composition (Example 5) were added to 99.9875, 99.9935, and 99.9969 grams of the culture media, respectively, and stirred evenly, resulting in concentrations of 0.0125%, 0.0065%, and 0.0031%, respectively.

[0153] 3) Drug administration: When the cell deposition rate in the 48-well plate reached 40%–60%, the 3% maltose medium and the three sample groups were administered drugs. For the negative control group, 500 μL of the medium containing 99.5% purified water prepared in step 2) was added to each well; for the positive control group, 500 μL of the medium containing 3% maltose prepared in step 2) was added to each well; for the sample groups, 500 μL of the sample medium prepared in step 2) at concentrations of 0.0125%, 0.0065%, and 0.0031% were added to each well. After drug administration, the 48-well plates were placed in an incubator (37℃, 5% CO2) and incubated for 12 hours.

[0154] 4) Remove the culture medium and store the cells at room temperature for 3 hours. At 1 hour, 2 hours and 3 hours, read the OD value (at 490 nm). Calculate the relative cell viability according to the formula. The results are shown in Table 10.

[0155] Relative cell viability = (OD of sample group / OD of negative control group) × 100%;

[0156] Table 10 Results of Cell Viability Test

[0157]

[0158] The experimental results show that:

[0159] 1. Cell viability varies considerably in different culture media.

[0160] 2. Compared with purified water culture medium and maltose culture medium, the cell viability of the culture medium containing the anti-environmental pollution skin care composition was not significantly different from that of the positive control group (maltose culture medium), indicating that the skin care composition has good cell viability.

[0161] The above experiments show that the anti-pollution composition can protect cell survival and can be used in skin care products to help protect cells from damage caused by climate change, such as dehydration and temperature changes.

[0162] Table 10 shows the test cells as human fibroblasts. Because carbohydrates provide energy for cells, cell culture media often use maltose and fructose as a matrix and a source of cell nutrition. Without a carbohydrate matrix, cells will struggle to survive. Using maltose culture medium as a positive control fully demonstrates that the components of this invention are not only harmless to cells but also, like maltose, can serve as a carbon source to provide cellular nutrients. This allows skin cells to obtain nutrients and continue to survive in polluted environments, preventing irreversible damage caused by pollutants penetrating the body due to skin cell death.

[0163] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any equivalent substitutions or modifications made by those skilled in the art within the spirit and principle of the present invention without any creative effort should be included within the protection scope of the present invention.

Claims

1. A skincare composition for combating environmental pollution, comprising, by weight percentage, 1-8% macadamia seed oil, 2-8% jojoba seed oil, 1-6% squalane, 2-5% shea butter, and 0.1-0.5% sodium hyaluronate, characterized in that: It also includes 0.1-2% bisabolol, 0.1-2% rice germ extract and rice extract mixture, 0.1-4% Chondrus crispus extract and Lithops spurius extract mixture, 0.1-3% Micrococcus iris extract, 0.1-4% olive fruit water and olive fruit extract mixture, 0.1-2.5% bioflavonoids, 6-49% excipients, and water as the balance; The rice germ extract and rice extract mixture is BLUE Oléoactif ® ; The mixture of *Chlorophytum comosum* extract and *Lithocarpus septum* extract is OLIGOGELINE™ SPE; PEPHA is an extract of Micrococcus oxyphylla with eye-like veins. ® -TIGHT CB; The mixture of olive fruit water and olive fruit extract is called ITALINE. ® O; The bioflavonoid is elixiance™ biofunctional.

2. The skincare composition for combating environmental pollution according to claim 1, characterized in that: The excipients include at least an emulsifier, which is a stratum corneum lipid-like substance, and the stratum corneum lipid-like substance accounts for 2-12% of the weight of the skin care composition; the stratum corneum lipid-like substance is ProLipid. TM 141 lamellar gel.

3. The skincare composition for resisting environmental pollution according to claim 2, characterized in that: The excipients also include preservatives, emollients, skin conditioning agents, and thickeners; the preservative is ethylhexylglycerin / glyceryl caprylate, the emollient is diethylhexyl carbonate, the skin conditioning agents are 1,2-pentanediol and arginine, and the thickener is sodium polyacrylamide dimethyl taurate.

4. The skincare composition for resisting environmental pollution according to claim 3, characterized in that: The ethylhexylglycerin / glyceryl caprylate accounts for 0.5-1.0% of the weight of the skin care composition, the mass ratio of ethylhexylglycerin to glyceryl caprylate in the ethylhexylglycerin / glyceryl caprylate is 1:1, the diethylhexyl carbonate is 2.5-5%, 1,2-pentanediol is 1-5%, arginine is 0.01-0.05%, and sodium polyacrylamide dimethyl taurate is 0.2-0.6%.

5. A method for preparing the anti-environmental pollution skincare composition according to claim 3 or 4, characterized in that, Includes the following steps: (1) Add excipient A and water as phase A raw material to a vacuum emulsifying pot and dissolve them at 80±3℃ under high speed and low speed double stirring in the vacuum emulsifying pot for at least 20 min to obtain phase A; (2) Sodium hyaluronate and water are used as phase B raw materials and stirred in a mixing pot at 800±50 rpm for at least 20 min to be completely dispersed. Then they are added to the vacuum emulsification pot of step (1) and dissolved at 80±3℃ under high speed and low speed double stirring in the vacuum emulsification pot for at least 20 min to obtain phase B. (3) In another mixing pot, the whole-leaved macadamia seed oil, auxiliary material B, jojoba seed oil, squalane, shea butter and bisabolol are used as raw materials for phase C. Heat to 80±3℃ and stir until fully dissolved. Stir at 250±50 rpm for at least 20 minutes to obtain phase C. (4) Inject the C phase in the mixing pot in step (3) into the vacuum emulsifying pot in step (2) for emulsification; (5) Perform emulsification in the main pot of the vacuum emulsifying pot; (6) Stir and cool the vacuum emulsifying pot to 30°C; (7) Add rice germ extract and rice extract mixture, wrinkled hornweed extract and spurious algae extract mixture, eye-like vein microphobic algae extract, olive fruit water and olive fruit extract mixture, and bioflavonoids as D phase raw materials to the vacuum emulsifying pot of step (6) and stir. (8) Degas and filter, and the product is ready for discharge; The excipient A is a skin conditioning agent and / or a thickener; the excipient B is one or more of the following: emollient, preservative, and emulsifier.

6. The method for preparing the anti-environmental pollution skincare composition according to claim 5, characterized in that: In steps (1) and (2), the stirring speeds of the vacuum emulsifying pot are: 500±50 rpm for high-speed stirring and 60±5 rpm for low-speed stirring. The mass ratio of the water added in step (1) to the water added in step (2) is 2.0 to 3.0:1; The conditions for emulsification in the vacuum emulsifying pot in step (4) are: temperature: 80±3℃, low speed: 60±5 rpm, high speed: 1500±50 rpm, time: 10~15 minutes; The conditions for emulsification in the main pot in step (5) are: temperature: 80±3℃, low speed: 60±5 rpm, high speed: 2000±50 rpm, and time: 20-30 minutes; In step (6), cooling to 30°C specifically involves using a vacuum emulsifying pot with a low speed of 60±5 rpm, a high speed of 0 rpm, and a time of 30 to 50 minutes. Once the temperature reaches 30°C, the process is stopped. In step (7), the D phase raw material is added to the vacuum emulsifying pot and stirred as follows: low speed 60±5 rpm, high speed 0 rpm, temperature 25~30℃ for 20 minutes.

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