A soothing repair composition and uses, cosmetic

Abstract

The application belongs to the field of cosmetics, and discloses a soothing and repairing composition and application and a cosmetic, wherein the composition is composed of camellia flower extract, dictamnus root bark extract and paeonia root extract, the camellia flower extract, the dictamnus root bark extract and the paeonia root extract are respectively extracted from 0.5-5 parts of camellia flower, 1-8 parts of dictamnus root bark and 0.5-10 parts of paeonia root; through the combined use of the three, a synergistic effect can be achieved, and the soothing and repairing effect of the composition can be further improved; in addition, the application discloses a cosmetic containing 0.01-90 wt% of the soothing and repairing composition.

Description

Technical Field

[0001] This application relates to the field of cosmetics, and more particularly to a soothing and repairing composition and its use, and cosmetics. Background Technology

[0002] Nowadays, people are paying more and more attention to skin care, and maintaining good skin condition has become a goal for many. However, in modern life, various adverse factors affect skin health, such as climate change, environmental pollution, and changes in people's lifestyles, such as irregular work and rest and mental stress. These can easily lead to skin sensitivity problems, which are becoming increasingly common among the population.

[0003] When skin is sensitive, it often causes discomfort such as redness, tightness, itching, and stinging. The reasons behind this are complex and may be related to skin inflammation, nerve stimulation, and a weakened skin barrier. When the skin is affected by external factors, the body may produce substances that trigger discomfort, leading to various sensitivity symptoms. At the same time, if the skin's protective layer is damaged, its resistance to external stimuli decreases, making the sensitivity more pronounced.

[0004] Many skin-soothing and repairing cosmetics on the market today contain plant-based ingredients, which can make the skin feel more comfortable and reduce discomfort to some extent. There are also some natural plants traditionally considered beneficial to the skin whose value has not yet been fully utilized in modern cosmetic development, and whose potential remains largely untapped.

[0005] The technical problem to be solved by this application is: how to provide a soothing and repairing composition based on plant ingredients. Summary of the Invention

[0006] To address the aforementioned issues, the purpose of this application is to provide a soothing and repairing composition and its uses, as well as a cosmetic, which achieves a soothing and repairing effect through the synergistic effect of a combination of camellia extract, dictamnus root bark extract, and peony root extract.

[0007] To achieve the above objectives, this application discloses a soothing and repairing composition, which, by weight, comprises 0.5 to 5 parts of camellia flower extract, 1 to 8 parts of Dictamnus dasycarpus root bark extract, and 0.5 to 10 parts of peony root extract.

[0008] Preferably, the extraction method of the camellia flower extract is as follows:

[0009] The extraction method of the camellia flower extract is as follows: after the camellia flower is crushed into powder, it is added to a 70wt% ethanol solution with 8 to 12 times its own weight, stirred and extracted at 55±5℃ for 2 to 3 hours to obtain camellia flower extract.

[0010] The camellia extract was then concentrated to an alcohol-free state. 0.5-1 wt% chitosan was added to the camellia extract for flocculation and impurity removal. The supernatant was collected by centrifugation, and the pH was slowly adjusted to 7.0-8.0 with 1 wt% sodium hydroxide solution. After standing and centrifugation, the supernatant was collected to obtain the camellia extract.

[0011] Preferably, the extraction method of the Dictamnus dasycarpus root bark extract is as follows:

[0012] After crushing the root bark of Dictamnus dasycarpus into powder, add 10 to 20 times its own weight of 0.1 wt% citric acid aqueous solution, stir and extract at 50±5℃ for 2 to 3 hours, centrifuge and filter, and collect the filtrate to obtain the extract of Dictamnus dasycarpus root bark.

[0013] The extract of Dictamnus dasycarpus root bark was then passed through a D101 macroporous adsorption resin and desorbed with a 70wt% ethanol solution. The desorbed solution was then concentrated to an alcohol-free state to obtain the extract of Dictamnus dasycarpus root bark.

[0014] Preferably, the extraction method of the peony root extract is as follows: after pulverizing the peony root into powder, add 15 to 20 times its own weight of 50wt% ethanol solution, stir and extract at 60±5℃ for 2 to 4 hours, centrifuge and filter, and take the filtrate to obtain peony root extract.

[0015] The peony root extract was concentrated to an alcohol-free state to obtain the peony root extract.

[0016] In addition, this application also discloses a method for preparing the above-mentioned soothing and repairing composition, wherein camellia flower extract, Dictamnus dasycarpus root bark extract and Paeonia lactiflora root extract are mixed to obtain the soothing and repairing composition.

[0017] Preferably, camellia flower extract, Dictamnus dasycarpus root bark extract, and Paeonia lactiflora root extract are mixed and dissolved in a polyol to obtain a mixed solution; the polyol content in the mixed solution is 20-50 wt%.

[0018] The mixed solution was then stirred, extracted, and ultrafiltered to obtain the soothing and repairing composition.

[0019] Preferably, the stirring extraction is performed at room temperature and 600-750 rpm for 0.5-1 h; the ultrafiltration is performed at an ultrafiltration pressure of 0.20-0.30 MPa, with the ultrafiltration temperature controlled to not exceed 35°C and the ultrafiltration membrane pore size between 0.1 and 0.45 μm.

[0020] Preferably, the polyol is at least one selected from butanediol, glycerol, methylpropanediol, and 1,3-propanediol.

[0021] In addition, this application also discloses the use of the above-described soothing and repairing composition in the preparation of cosmetics.

[0022] In addition, this application also discloses a cosmetic product containing 0.01 to 90 wt% of the soothing and repairing composition as described above.

[0023] The beneficial effects of this invention are:

[0024] This invention provides a soothing and repairing composition and its uses, as well as a cosmetic product. The composition combines camellia flower extract, dictamnus root bark extract, and peony root extract. Camellia flower extract inhibits inflammatory factors, thus reducing redness and stinging; dictamnus root bark extract has good hyaluronidase inhibitory ability, providing a good soothing effect; and peony root extract also has good inflammatory factor inhibitory ability. The three ingredients work synergistically to achieve a soothing and repairing effect. Attached Figure Description

[0025] Figure 1 An observation image of a chicken embryo before using the soothing and repairing composition of Example 2 of the present invention;

[0026] Figure 2 An observation image of a chicken embryo after using the soothing and repairing composition of Example 2 of the present invention. Detailed Implementation

[0027] In the description of this invention, it should be noted that unless specific conditions are specified in the examples, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0028] Supplier information and / or product information

[0029] Camellia flowers: purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., with a moisture content of 4.3%;

[0030] Dictamnus dasycarpus root bark: purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., with a moisture content of 12.5%;

[0031] Peony root: The commercial name is Red Peony Root, which was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., and the moisture content is 10.3%.

[0032] Preparation method of soothing and repairing compositions (unless otherwise specified, the compositions in the following examples and comparative examples are all prepared using this method):

[0033] Step a: After crushing the camellia flowers into powder, add 10 times the mass of the 70wt% ethanol solution (10 times the mass of the solvent, i.e., the 70wt% ethanol solution, is 10 times the mass of the camellia flower powder; the following descriptions can be understood by referring to this point) and stir and extract at 55±5℃ for 2 hours to obtain camellia flower extract.

[0034] Step b: After concentrating the camellia extract to a non-alcoholic state, based on the quality of the concentrated camellia extract, add 1wt% chitosan to flocculate and remove impurities, centrifuge and collect the supernatant, then slowly adjust the pH to 7.0-8.0 with 1wt% sodium hydroxide solution, then let it stand for 30 minutes, and centrifuge again to collect the supernatant to obtain the camellia extract.

[0035] Step c: After crushing the root bark of Dictamnus dasycarpus into powder, add 15 times the weight of 0.1wt% citric acid aqueous solution, stir and extract at 50±5℃ for 2.5h, centrifuge and filter, and take the filtrate to obtain the root bark extract of Dictamnus dasycarpus.

[0036] Step d: Pass the extract of Dictamnus dasycarpus root bark through D101 macroporous adsorption resin and elute with 70wt% ethanol as the eluent until the eluent is colorless. Stop elution, desorption is complete, and the eluent is obtained.

[0037] Step e: Concentrate the desorption solution to an alcohol-free state to obtain Dictamnus dasycarpus root bark extract;

[0038] Step f: After crushing the peony root into powder, add 18 times the mass of 50wt% ethanol, stir and extract at 60±5℃ for 3h, centrifuge and filter, and take the filtrate to obtain peony root extract.

[0039] Step g: Concentrate the peony root extract under reduced pressure until it is alcohol-free to obtain peony root extract;

[0040] Step h: Camellia flower extract, Dictamnus dasycarpus root bark extract, and Paeonia lactiflora root extract are mixed and dissolved in butylene glycol to obtain a mixed solution; the content of polyol (butylene glycol) in the mixed solution is 33.8 wt%.

[0041] Step i: Extract the mixed solution at room temperature and 700 rpm for 1 hour by stirring. The specific operation of ultrafiltration is as follows: under an ultrafiltration pressure of 0.25 MPa, control the ultrafiltration temperature to not exceed 35°C, and ultrafiltration membrane pore size to be 0.2 μm. After ultrafiltration, take the filtrate to obtain the soothing and repairing composition.

[0042] Examples 1-3

[0043] A soothing and repairing composition, the raw materials and their quality ratios are shown in Table 1:

[0044] Table 1 Formulation table for Examples 1-3

[0045] Group Camellia extract Dictamnus dasycarpus root bark extract Peony root extract Example 1 0.5 8 10 Example 2 3 4 5 Example 3 5 1 0.5

[0046] Example 4

[0047] The method is basically the same as in Example 2, except that the preparation method of the soothing and repairing composition is as follows:

[0048] Step a: After crushing the camellia flowers into powder, add them to 10 times their weight of 70wt% ethanol solution and stir and extract at 55±5℃ for 2 hours to obtain camellia flower extract.

[0049] Step b: After concentrating the camellia extract to a non-alcoholic state, based on the quality of the concentrated camellia extract, add 1wt% chitosan to flocculate and remove impurities, centrifuge and collect the supernatant, slowly adjust the pH to 7.0~8.0 with 1wt% sodium hydroxide solution, let stand for 30 minutes, centrifuge again and collect the supernatant to obtain the camellia extract.

[0050] Step c: After crushing the root bark of Dictamnus dasycarpus into powder, add 15 times the weight of 0.1wt% citric acid aqueous solution, stir and extract at 50±5℃ for 2.5h, centrifuge and filter, and take the filtrate to obtain the root bark extract of Dictamnus dasycarpus.

[0051] Step d: Pass the extract of Dictamnus dasycarpus root bark through D101 macroporous adsorption resin and elute with 70wt% ethanol as the eluent until the eluent is colorless. Stop elution, desorption is complete, and the eluent is obtained.

[0052] Step e: Concentrate the desorption solution to an alcohol-free state to obtain Dictamnus dasycarpus root bark extract;

[0053] Step f: After crushing the peony root into powder, add 18 times the mass of 50wt% ethanol, stir and extract at 60±5℃ for 3h, centrifuge and filter, and take the filtrate to obtain peony root extract.

[0054] Step g: Concentrate the peony root extract under reduced pressure until it is alcohol-free to obtain peony root extract;

[0055] Step h: Camellia extract, Dictamnus dasycarpus root bark extract, and Paeonia lactiflora root extract are mixed and dissolved in butylene glycol to obtain a soothing and repairing composition, wherein the content of polyol (butylene glycol) in the soothing and repairing composition is 33.8 wt%.

[0056] Comparative Examples 1-6

[0057] A composition, the raw materials and their mass ratios are shown in Table 2:

[0058] Table 2 Formulation table for Comparative Examples 1-6

[0059] Group Camellia extract Dictamnus dasycarpus root bark extract Peony root extract Comparative Example 1 3 0 0 Comparative Example 2 0 4 0 Comparative Example 3 0 0 5 Comparative Example 4 0 4 5 Comparative Example 5 3 0 5 Comparative Example 6 3 4 0

[0060] Performance testing:

[0061] I. Hyaluronidase Inhibition Rate Test

[0062] 1. Experimental Objective and Principle

[0063] Hyaluronidase is a specific enzyme that cleaves hyaluronic acid and is involved in allergic reactions, showing a strong correlation with histamine release from mast cells. The soothing effect of a test sample can be determined using the hyaluronidase inhibition rate; a higher inhibition rate indicates a stronger soothing effect, and vice versa.

[0064] 2. Experimental Materials and Methods

[0065] 2.1 Instruments and equipment: BSA224S analytical balance, L6s ultraviolet spectrophotometer;

[0066] 2.2 Reagents: BR grade hyaluronidase, BR grade sodium hyaluronate.

[0067] 2.3 Test Methods

[0068] (1) Treatment of control materials and test samples

[0069] Sample group: Dilute the compositions obtained in Examples 1-4 and Comparative Examples 1-6 with pure water to a concentration of 5%;

[0070] Positive control group (dipotassium glycyrrhizate, purity ≥98%): diluted with pure water to a positive control concentration of 3%;

[0071] Negative control: pure water.

[0072] (2) Experimental operation procedures

[0073] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group should have 3 replicates. Add different reagent solutions to each of the four groups, shake well, and let stand at room temperature for 30 minutes to develop color. Measure the absorbance value at a wavelength of 528 nm using a UV spectrophotometer.

[0074] (3) Calculation formula

[0075] % Formula 1;

[0076] In the formula:

[0077] A—Absorbance of the sample-free and enzyme-containing reaction solutions;

[0078] B—The absorbance of the reaction solution without the sample and without the enzyme;

[0079] C—is the absorbance of the sample and the reaction solution containing the enzyme;

[0080] D—The absorbance of the reaction solution containing the sample and the reaction solution without the enzyme.

[0081] II. Measurement of UVB-induced keratinocyte-inflammatory factor IL-8 content

[0082] 1. Experimental Objective and Principle

[0083] The skin is the most vulnerable immune organ to damage after ultraviolet (UV) radiation. UVB can induce skin immunosuppression by directly or indirectly inducing the release of cytokines from skin cells. Keratinocytes are UVB-sensitive target cells and, after exposure to UVB, can secrete various cytokines such as IL-8, TNF-α, IL-1α, PGE2, IL-1β, and IL-6, which participate in various inflammatory responses.

[0084] This experiment followed laboratory methods, comparing the results of cellular IL-8 content measurement between the test sample and the negative control. If the cellular IL-8 content of the test sample was lower than that of the negative control, the test sample could be considered to have a certain soothing effect.

[0085] This experimental method is an in vitro method that provides a method for detecting the IL-8 content in UVB-induced keratinocytes, which can serve as one of the pieces of evidence supporting the claims of soothing efficacy in cosmetic raw materials and finished products.

[0086] 2. Experimental Materials and Methods

[0087] 2.1 Instruments and equipment: RT-6100 enzyme-linked immunosorbent assay (ELISA) analyzer, ultraviolet lamp (302nm).

[0088] 2.2 Cells used in the experiment: keratinocytes.

[0089] 2.3 Test Methods

[0090] (1) Sample preparation

[0091] Sample group: The sample (the composition prepared in the examples and comparative examples) was diluted with pure water to a 2wt% solution, filtered through a 0.22 μm filter, and the filtrate was collected as the sample mother liquor;

[0092] Negative control: basal culture medium (cell viability assay).

[0093] (2) Experimental operation procedures

[0094] Cell viability assay: Keratinocytes were seeded into 96-well plates. After 24 h, the culture medium was discarded, and basal culture medium containing different test samples was added. After 24 h, OD was measured by MTT assay. 490nm, and the effect of the test sample on the activity of keratinocytes was calculated.

[0095] IL-8 content assay: Cells were seeded in 96-well plates and cultured for 24 h. Culture medium containing 1 wt% of the sample (the stock solutions of the above samples were diluted with pure water to a 1% sample dilution) was added to the sample wells. Culture medium was added to the negative control wells and the normal control wells. Cells were incubated for 24 h. The supernatant was discarded, and the cells were washed three times with PBS. The normal control group was completely wrapped in aluminum foil and placed in the dark. Cells in the other irradiated groups were placed under UVB lamps with an irradiation dose of 90 mJ / cm². 2 After completion, discard the PBS solution, add culture medium, incubate for 4 hours, aspirate the supernatant, and use an ELISA kit to determine the IL-8 content in the cell culture supernatant to obtain the effect of the experimental sample on the IL-8 content of keratinocytes.

[0096] The calculation formula is shown in Equation 2-3:

[0097] Formula 2;

[0098] Formula 3;

[0099] The test results of hyaluronidase inhibition rate and IL-8 inhibition rate are shown in Table 3:

[0100] Table 3 Performance Test Results

[0101] Group Hyaluronidase inhibition rate / % IL-8 inhibition rate / % Example 1 39.85 23.25 Example 2 42.93 25.44 Example 3 37.02 22.67 Example 4 33.93 20.51 Comparative Example 1 12.85 7.42 Comparative Example 2 21.34 3.26 Comparative Example 3 17.48 10.77 Comparative Example 4 25.96 13.99 Comparative Example 5 22.88 14.32 Comparative Example 6 24.16 18.05 Positive control 70.95 / negative control -0.51 /

[0102] Results analysis:

[0103] 1. As can be seen from Examples 1-3, when the mass ratio between Camellia extract, Dictamnus dasycarpus root bark extract and Paeonia lactiflora root extract is adjusted slightly, the hyaluronic acid inhibition rate and the inflammatory factor IL-8 in Examples 1 to 3 show a certain degree of fluctuation, but the fluctuation range is relatively small.

[0104] Further observation of Example 4 shows that when the mixing method of camellia extract, dictamnus root bark extract and peony root extract was changed, the hyaluronidase inhibition rate and the ability to inhibit inflammatory factors in Example 4 showed a certain degree of decline. It can be seen that reducing the ultrafiltration and extraction operations after mixing may affect the quality of active substances or the content of impurities in the composition, thereby leading to a certain degree of decline in the hyaluronidase inhibition ability and anti-inflammatory ability of the composition without ultrafiltration and extraction under the same test volume.

[0105] 2. Observations of Comparative Examples 1-3 show that when camellia flower extract, Dictamnus dasycarpus root bark extract, and Paeonia lactiflora root extract are used alone, Dictamnus dasycarpus root bark extract has a better hyaluronidase inhibitory ability than camellia flower extract and Paeonia lactiflora root extract. However, the inflammatory factor inhibitory ability of Dictamnus dasycarpus root bark extract is significantly weaker than that of camellia flower extract and Paeonia lactiflora root extract. It can be seen that Dictamnus dasycarpus root bark extract plays a leading role in hyaluronidase inhibition, while camellia flower extract and Paeonia lactiflora root extract should make greater contributions to the inhibition of inflammatory factors.

[0106] Furthermore, further observation of Comparative Examples 4-6 revealed that when any two of Camellia Flower Extract, Dictamnus dasycarpus Root Bark Extract, and Paeonia lactiflora Root Extract were used in Comparative Examples 4-6, the hyaluronidase inhibition rate and inflammatory factor inhibition rate of the three extracts showed a more significant upward trend compared to the use of Dictamnus dasycarpus Root Bark Extract, Camellia Flower Extract, and Paeonia lactiflora Root Extract alone.

[0107] It is evident that any two of the extracts from Dictamnus dasycarpus root bark, Camellia japonica flower, and Paeonia lactiflora root have a certain synergistic effect, thereby enhancing the hyaluronidase inhibition and inflammatory factor inhibition capabilities of the comparative ratios 4-6.

[0108] However, taking the hyaluronidase inhibition rate as an example, further calculation of the synergistic effect of the three extracts reveals that the actual value of Comparative Example 4 is about 6.77 higher than the theoretical value (the theoretical value of Comparative Example 4 is calculated as: hyaluronidase inhibition rate of Comparative Example 2 × 4 / 9 + hyaluronidase inhibition rate of Comparative Example 3 × 5 / 9 ≈ 9.48 + 9.71 = 19.19).

[0109] The actual value of Comparative Example 4 reached 25.96, which shows that the difference between the actual value and the theoretical value is about 6.77. This value can be identified as the contribution value obtained by the synergistic effect between Dictamnus dasycarpus root bark extract and Paeonia lactiflora root extract.

[0110] Similarly, calculations based on Comparative Example 5, Comparative Example 1, and Comparative Example 3 show that the synergistic contribution value between Camellia extract and Paeonia suffruticosa root extract is approximately 7.14.

[0111] Calculations based on Comparative Example 6 and Comparative Examples 1 and 2 show that the synergistic contribution value between Camellia extract and Dictamnus dasycarpus root bark extract is approximately 6.46.

[0112] The theoretical value of Example 2 can be seen from the calculation (the calculation method is: hyaluronidase inhibition rate of Comparative Example 1 × 3 / 12 + hyaluronidase inhibition rate of Comparative Example 2 × 4 / 12 + hyaluronidase inhibition rate of Comparative Example 3 × 5 / 12 + synergistic contribution value of Comparative Example 4 × 9 / 12 + synergistic contribution value of Comparative Example 5 × 8 / 12 + synergistic contribution value of Comparative Example 6 × 7 / 12 ≈ 3.21 + 7.11 + 7.28 + 5.08 + 4.76 + 3.77 = 31.21).

[0113] Furthermore, considering the actual values ​​in Example 2, the difference between the actual value and the theoretical value is 42.93 - 31.21 = 11.72.

[0114] It is evident that the actual value of Example 2 far exceeds its theoretical value;

[0115] This shows that when Dictamnus dasycarpus root bark extract, Camellia japonica flower extract, and Paeonia lactiflora root extract are used together, the synergistic effect between the raw materials can be further amplified.

[0116] III. Stimulation Tests

[0117] Test method: Chicken embryo chorioallantoic membrane test

[0118] 1. Experimental Objective and Principle

[0119] The chorioallantoic membrane (CAM) test is an early and widely used in vitro method for assessing eye irritation. The CAM is a respiratory membrane surrounding the chicken embryo. This test utilizes the intact, clear, and transparent vascular system of the mid-stage chorioallantoic membrane in hatched chicken embryos. A certain amount of the test substance is directly exposed to the CAM, and after a period of time, changes in CAM toxicity indicators (such as hemorrhage, coagulation, and vascularization) are observed. These indicators reflect changes in the morphology, color, and permeability of blood vessels and vascular networks, as well as phenomena such as CAM protein denaturation and the degree of damage. These indicators are then combined to obtain a score used to assess the eye irritation of the test substance.

[0120] The purpose of this study is to test the ability of the test substance to cause toxic changes in the chorioallantoic membrane of chicken embryos and to evaluate the elements and processes of the potential eye irritation of the substance being evaluated.

[0121] 2. Experimental Materials and Methods

[0122] 2.1 Instruments and equipment: fully automatic incubator, stereomicroscope, SPF chicken embryos.

[0123] 2.2 Reagents: Sodium chloride, sodium dodecyl sulfonate (SDS).

[0124] 2.3 Incubation conditions: Room temperature 20-25℃, relative humidity 45-70%. Incubation temperature 37.5±0.5℃, relative humidity 55-70%, rotating tray 3-6 times / h. Rotation is not necessary for 9-day-old chicken embryos during incubation.

[0125] 3. Experimental Procedure

[0126] In this test, 6 chicken embryos were selected for each group. The condition of the chorioallantoic membrane was recorded using a photographic device. The sample to be tested was added to the chorioallantoic membrane of the chicken embryo, the time of sample addition was recorded, and the air cell was covered with a moistened plastic wrap. The chicken embryos were then transferred to a constant temperature and humidity incubator for culture. The degree of change of each toxic effect was observed and photographed.

[0127] 4. Results Observation

[0128] Observe and record bleeding, coagulation, and vascular dissolution, and score them according to their severity.

[0129] 5. Result Judgment Criteria

[0130] ES≤4, non-irritating;

[0131] 4 < ES ≤ 12, mild irritation;

[0132] 12 < ES < 16, moderately irritating;

[0133] ES≥16, highly irritating / corrosive.

[0134] 6. Test Results

[0135] The sample of Example 2 was tested according to the above test method. ES=2.00, and the result was determined to be non-irritating.

[0136] Application Example 1

[0137] A soothing and repairing product is prepared according to the formula in Table 4. The preparation method is as follows: It should be noted that the sample containing Example 2 is prepared by mixing all raw materials according to the formula in Table 4 and sterilizing at 85±3℃ for 1 hour.

[0138] Table 4 Ingredient list of soothing and repairing products

[0139] Raw material name Amount added (wt%) The composition obtained in Example 2 88.7 glycerin 10 1,2-Hexanediol 0.8 1,2-Pentanediol 0.5

[0140] Application Example 2

[0141] A basic soothing and repairing product is prepared according to the formula in Table 5. The preparation method is as follows:

[0142] Step 1: Pre-prepared phase: Dissolve phase B in advance and stir until homogeneous; mix phase C in advance and stir until homogeneous, set aside.

[0143] Step 2: Main pot: Add the pure water from phase A to the homogenizer, turn on the homogenizer, and while homogenizing, add the remaining raw materials from phase A sequentially, homogenizing at medium speed for 50 seconds. Stir and heat to 82±2℃, homogenize at medium speed for 2 minutes. Continue until no gel particles remain. Keep warm for 30 minutes, then stir and cool.

[0144] Step 3: Cool down to 50℃, add phases B and C in sequence, and stir well;

[0145] Step 4: Cool down to 45℃, add phase D, and stir well.

[0146] Table 5. Basic Soothing and Repairing Product Formula Table

[0147]

[0148] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. A soothing and repairing composition, characterized in that, The soothing and repairing composition, by weight, comprises 0.5–5 parts of camellia flower extract, 1–8 parts of Dictamnus dasycarpus root bark extract, and 0.5–10 parts of peony root extract, mixed and dissolved in a polyol to obtain a mixed solution; the polyol content in the mixed solution is 20–50 wt%. The mixed solution was then stirred, extracted, and ultrafiltered to obtain a soothing and repairing composition. The extraction method for the camellia flower extract is as follows: The extraction method of the camellia flower extract is as follows: after the camellia flower is crushed into powder, it is added to a 70wt% ethanol solution with 8 to 12 times its own weight, stirred and extracted at 55±5℃ for 2 to 3 hours to obtain camellia flower extract. The camellia extract was then concentrated to an alcohol-free state. 0.5-1 wt% chitosan was added to the camellia extract for flocculation and impurity removal. The supernatant was then collected by centrifugation. The pH was slowly adjusted to 7.0-8.0 with 1 wt% sodium hydroxide solution. After standing and centrifugation, the supernatant was collected to obtain the camellia extract. The extraction method of the Dictamnus dasycarpus root bark extract is as follows: After crushing the root bark of Dictamnus dasycarpus into powder, add 10 to 20 times its own weight of 0.1 wt% citric acid aqueous solution, stir and extract at 50±5℃ for 2 to 3 hours, centrifuge and filter, and collect the filtrate to obtain the extract of Dictamnus dasycarpus root bark. The extract of Dictamnus dasycarpus root bark was then passed through D101 macroporous adsorption resin and desorbed with 70wt% ethanol solution. The desorbed solution was then concentrated to an alcohol-free state to obtain Dictamnus dasycarpus root bark extract. The extraction method of the peony root extract is as follows: after pulverizing the peony root into powder, add 15 to 20 times its own weight of 50wt% ethanol solution, stir and extract at 60±5℃ for 2 to 4 hours, centrifuge and filter, and take the filtrate to obtain peony root extract. The peony root extract was concentrated to an alcohol-free state to obtain the peony root extract.

2. A method for preparing the soothing and repairing composition according to claim 1, characterized in that, Camellia flower extract, Dictamnus dasycarpus root bark extract, and Paeonia lactiflora root extract were mixed and dissolved in a polyol to obtain a mixed solution; the polyol content in the mixed solution was 20-50 wt%. The mixed solution was then stirred, extracted, and ultrafiltered to obtain the soothing and repairing composition.

3. The method for preparing the soothing and repairing composition according to claim 2, characterized in that, The specific operation of the stirring extraction is as follows: stirring extraction at 600-750 rpm for 0.5-1 h at room temperature; the specific operation of the ultrafiltration is as follows: ultrafiltration at an ultrafiltration pressure of 0.20-0.30 MPa, controlling the ultrafiltration temperature not to exceed 35℃, and ultrafiltration membrane pore size of 0.1-0.45 μm.

4. The method for preparing the soothing and repairing composition according to claim 2, characterized in that, The polyol is at least one of butanediol, glycerol, methylpropanediol, and 1,3-propanediol.

5. Use of the soothing and repairing composition according to claim 1 in the preparation of cosmetics.

6. A cosmetic product, characterized in that, The cosmetic contains 0.01 to 90 wt% of the soothing and repairing composition as described in claim 1.

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