Essential oil composition extracted from Chinese herbal medicines, having killing effect on neisseria gonorrhoeae and suitable for treating urogenital system infection and pharyngolaryngitis

Abstract

The invention relates to an essential oil composition extracted from Chinese herbal medicines, which has a killing effect on neisseria gonorrhoeae and is suitable for treating urogenital system infection and pharyngolaryngitis. The liquid medicine is prepared from the following Chinese herbal medicine essential oil in parts by weight: 6-40 parts of peppermint essential oil, 12-31 parts of oregano essential oil, 14-80 parts of eucalyptus oil, 10-65 parts of chamomile essential oil, 15-75 parts of tea tree essential oil, 17-80 parts of balm essential oil and 9-27 parts of thyme essential oil. The invention relates to a Chinese herbal medicine powerful bactericidal composition with fragrant smell, and the bactericidal rate gt of neisseria gonorrhoeae; the yield is 99.97%. The traditional Chinese medicine composition is safe to use, has no irritation to skin mucous membrane tissues, also has a lubricating effect, and creates another new chapter in China and even in the world in the technical field of medicines for treating infectious diseases by utilizing the safe and powerful bactericidal and antibacterial characteristics of Chinese herbal medicines. The method can be widely applied to the fields of medicines, health care products, antibacterial and bacteriostatic hygienic products and the like.

Description

Technical Field

[0001] This invention relates to an essential oil composition extracted from traditional Chinese medicine, which is effective against Neisseria gonorrhoeae and suitable for treating urinary tract infections and pharyngitis. The essential oil composition extracted from these traditional Chinese medicines exhibits a bactericidal rate of >99.97% against Neisseria gonorrhoeae, demonstrating a potent bactericidal effect. This invention utilizes the broad-spectrum antibacterial and antiviral pharmacological properties and unique safety of the volatile components of certain traditional Chinese medicines, opening a new chapter in the field of pharmaceutical technology for treating infectious diseases using the safe and potent bactericidal and antibacterial properties of traditional Chinese medicines, both in my country and globally.

[0002] This invention is used to treat infections of the male and female genitourinary systems and pharyngitis. It boasts high patient compliance, rapid clinical onset, and a short treatment course. Furthermore, it is non-irritating to the skin and mucous membranes, providing lubrication during sexual activity and facilitating the prevention and control of sexually transmitted infections. This invention is safe to use and has broad application prospects, potentially finding wide applicability in pharmaceuticals, health products, and antibacterial and bacteriostatic hygiene products. Background Technology

[0003] Neisseria gonorrhoeae is a Gram-negative bacterium and the pathogen of gonorrhea, a sexually transmitted infection. It commonly resides in the epithelial cell layer of the mucous membranes of the human urogenital tract, causing purulent infections of the urogenital system, as well as infections in other areas besides the male genitalia (pharynx, anus, rectum, conjunctiva, etc.). If left untreated, it can lead to a series of serious complications and sequelae, such as urethritis, prostatitis, epididymitis in men, vaginitis, cervicitis, pelvic inflammatory disease, salpingitis, ectopic pregnancy, or infertility in women. It can also promote the infection and spread of HIV, herpes simplex virus, Chlamydia trachomatis, and syphilis. Currently, there is no effective vaccine for Neisseria gonorrhoeae; treatment primarily involves antibiotics, but the bacteria readily evolve resistance.

[0004] In the fight against bacteria, the overuse of antibiotics has led to an increasing number and variety of drug-resistant bacteria, resulting in the emergence of complex and multi-drug resistant super-drug pathogens. Furthermore, infectious diseases caused by super-drug resistant pathogens are showing an increasing trend year by year.

[0005] Neisseria gonorrhoeae has developed severe resistance to antibiotics and is now resistant to almost all antibiotics. Ceftriaxone is a recommended drug that can successfully cure Neisseria gonorrhoeae infection of the urogenital system and pharynx. It is an internationally recognized first-line drug. However, the susceptibility of ceftriaxone to Neisseria gonorrhoeae in some countries is declining. The results of seven drug resistance monitoring sites in my country from 2013 to 2016 showed that the low susceptibility rate of ceftriaxone to Neisseria gonorrhoeae has reached 10.8% [see reference: [3] Yin YP, Han Y, Dai XQ, et al. Susceptibility of Neisseria gonorrhoeae to azithromycin and ceftriaxone in China: A retrospective study of national surveillance data from 2013 to 2016 [J]. PLOS Medicine, 2018, 15.] The drug resistance rate of clinical Neisseria gonorrhoeae strains in a hospital in Hangzhou in 2019 even reached 30% [see reference: [4] Yan J, Chen Y, Yang F, et al. High percentage of theceftriaxone-resistant Neisseria gonorrhoeae FC428 clone among isolates from asingle hospital in Hangzhou, China[J]. Journal of AntimicrobialChemotherapy, 2021.].

[0006] More worryingly, the ceftriaxone-resistant strain FC428 (MIC = 0.5 mg / L), initially discovered in Japan, has successfully spread internationally. The most important characteristic of the FC428 clone, and the determining factor for its high ceftriaxone resistance, is its embedded penA allele 60.001. The global spread of the FC428 clone will have a significant impact on the development of future treatment guidelines in various countries. In China, the FC428 clone has successfully spread to several geographically different regions, including Beijing, Hangzhou, Suzhou, Changsha, Shanghai, and Chengdu, leading to frequent cases of initial treatment failure. This "successful ceftriaxone defense line" is becoming increasingly precarious. The WHO believes that gonorrhea may once again enter an era without a cure, making the development of new drugs an urgent priority.

[0007] Treatment methods for male and female genitourinary system infections and pharyngitis include injection, oral administration, and topical application, with patients generally preferring topical medications. Topical medications often consist of chemical drugs, combinations of traditional Chinese and Western medicine, and some traditional Chinese medicine suppositories, washes, gargles, and sprays. Topical chemical drugs often irritate the skin and mucous membranes, particularly the vaginal mucosa, hindering the recovery of inflammation and its complications. They may even cause mucosal lesions, such as lymphoid follicle hyperplasia, and lead to various side effects, including recurrence and drug resistance. Furthermore, due to the swallowing nature of the throat, topical chemical drugs are particularly problematic for throat use. Currently available traditional Chinese medicine suppositories, washes, sprays, and gargles only have antibacterial effects and do not kill pathogens.

[0008] Essential oils extracted from traditional Chinese medicine are volatile mixtures with a distinctive aromatic scent and natural biological activity. They are not only safe, highly biocompatible, and fast-acting, but also possess analgesic, antiviral, bactericidal, antibacterial, anti-inflammatory, antioxidant, antimutagenic, free radical scavenging, immune system enhancement, and multidrug resistance regulation effects. Their unique aromatic scent has strong penetrating power, reaching capillaries and nerve endings.

[0009] Utilizing the broad-spectrum antibacterial, antiviral, and antiviral properties of some traditional Chinese medicines, as well as their unique safety, as long as the formula is accurate, they are harmless and non-irritating to the skin and mucous membranes, and also have a lubricating, nourishing, and protective effect. Not only do they have a fast clinical onset, short treatment course, and high cure rate, but they can also be used in sexual activity to facilitate lubrication and the prevention and control of sexually transmitted infections. Summary of the Invention

[0010] An essential oil composition extracted from traditional Chinese medicine that has a killing effect on Neisseria gonorrhoeae and is suitable for treating urinary tract infections and pharyngitis. It is a medicinal liquid prepared from the following herbal essential oils in the following weight ratios: peppermint oil 6-40 parts, oregano oil 12-31 parts, eucalyptus oil (also known as eucalyptus oil, blue eucalyptus oil) 14-80 parts, chamomile oil 10-65 parts, tea tree oil (also known as Australian tea tree oil, Melaleuca alternifolia leaf oil, Melaleuca leucocephala leaf oil) 15-75 parts, lemon balm oil 17-80 parts, and thyme oil 9-27 parts.

[0011] Furthermore, this herbal essential oil combination solution is prepared from essential oils extracted from Chinese herbs in the following weight proportions: 6 parts peppermint essential oil, 12 parts oregano essential oil, 14 parts eucalyptus oil, 10 parts chamomile essential oil, 15 parts tea tree essential oil, 17 parts lemon balm essential oil, and 9 parts thyme essential oil.

[0012] Furthermore, it is a preparation made by adding the herbal essential oil combination liquid to pharmaceutical or food-grade excipients or auxiliary ingredients.

[0013] Furthermore, the preparation can be: oral spray, mouthwash, aromatherapy preparation, liquid preparation, oil preparation, drops, perfume preparation, ointment preparation, jelly preparation, tablet preparation, granule preparation, pill preparation, or soft candy preparation.

[0014] The present invention also provides the use of the aforementioned combination of essential oils extracted from traditional Chinese medicine in the preparation of drugs for gonococcal infection or male and female genitourinary system infections and pharyngitis.

[0015] This invention is an ingestible combination of essential oils extracted from traditional Chinese medicines that has a bactericidal effect against Neisseria gonorrhoeae. It has a strong and fragrant aroma, is non-irritating to the skin and mucous membranes, and also has a lubricating effect. It is suitable for external use to treat Neisseria gonorrhoeae infection, male and female urinary and reproductive system infections, and pharyngitis.

[0016] This invention utilizes the pharmacological properties of volatile components from traditional Chinese medicine to formulate a product. The essential oils from these herbs are the volatile components obtained through various extraction and distillation processes.

[0017] Peppermint essential oil is extracted from fresh peppermint leaves using an extraction and distillation process.

[0018] Peppermint has a pungent taste and cool properties. It is used for colds caused by wind-heat, sore throat, mouth ulcers, and toothache. Its pharmacological effects include antibacterial, antiviral, and antipruritic properties.

[0019] Oregano essential oil is extracted from fresh oregano leaves using an extraction and distillation process.

[0020] Oregano is pungent and warm in nature. It can induce sweating, relieve exterior syndromes, eliminate summer heat and dampness, and treat colds, heatstroke, and acute gastroenteritis. Its pharmacological effects include antimicrobial activity. Oregano volatile oil has antibacterial effects against various Shigella, Staphylococcus aureus and Staphylococcus albus, Escherichia coli, Salmonella typhi and Proteus.

[0021] Eucalyptus oil, also known as eucalyptus essential oil or blue eucalyptus leaf oil, is extracted from fresh blue eucalyptus leaves through an extraction and distillation process.

[0022] Eucalyptus oil has been prepared by distillation in traditional Chinese medicine. It is classified as a pungent and cool exterior-releasing medicine and is used clinically to treat colds, influenza, enteritis, diarrhea, and itchy skin. It can also treat burns and scalds. Pharmacologically, it has antibacterial effects and has a strong inhibitory effect on Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Shigella, etc.

[0023] Chamomile essential oil, made from fresh chamomile buds through an extraction and distillation process, is produced using chamomile, also known as mother chrysanthemum or European chamomile in traditional Chinese medicine.

[0024] Chamomile has a pungent, cool, and slightly bitter taste. It clears heat and detoxifies. Pharmacologically, it has anti-inflammatory effects, and its volatile oil has some disinfecting properties.

[0025] Tea tree oil, also known as Australian tea tree oil, Melaleuca alternifolia leaf oil, or Melaleuca leucocephala leaf oil, is extracted from its fresh branches and leaves through an extraction and distillation process.

[0026] Melaleuca lyrata leaves are pungent, astringent, and warm in nature. They are used to treat rheumatic bone pain, enteritis, and diarrhea, as well as allergic dermatitis, eczema, urticaria, rheumatic pain, neuralgia, and as an anthelmintic and antiseptic. Pharmacologically, they have inhibitory effects on Staphylococcus aureus and Escherichia coli. For details, please refer to the Guangzhou Military Region's "Handbook of Commonly Used Chinese Herbal Medicines" and "Chinese Materia Medica".

[0027] Lemon balm essential oil is extracted from fresh lemon balm leaves using an extraction and distillation process.

[0028] Lemon balm, also known as honey bee flower, lemon balm, peppermint balm, and balm grass in traditional Chinese medicine, has calming and sedative effects, aids digestion, and pharmacologically possesses antiviral properties, exhibiting a certain inhibitory effect on herpes virus.

[0029] Thyme essential oil is extracted from fresh thyme using an extraction and distillation process.

[0030] Thyme, also known as musk grass or ground pepper in traditional Chinese medicine, is used in its entirety and has antibacterial and expectorant effects. Its main pharmacological function is antibacterial.

[0031] Most Chinese herbal essential oils have broad-spectrum antibacterial and antiviral effects.

[0032] The essential oils extracted from the herbs in this invention, including peppermint oil, oregano oil, eucalyptus oil, chamomile oil, tea tree oil, lemon balm oil, and thyme oil, all possess antibacterial, anti-inflammatory, or antiviral effects according to modern pharmacology of traditional Chinese medicine efficacy. These effects are due to the presence of certain aromatic volatile substances in these herbs, collectively referred to as essential oils.

[0033] Herbal essential oils are mainly divided into two types: pure essential oils, which are insoluble in water; and water-soluble essential oils, which are soluble in water. (Of course, there is also a type of substance that is extracted from herbal essential oils using further technological means.)

[0034] Water-soluble essential oils are essential oils with added pharmaceutical or food-grade additives, such as those using Tween 80 or PEG-60 hydrogenated castor oil to enhance their water solubility. Water-soluble essential oils are raw materials for preparing aqueous pharmaceutical solutions.

[0035] The essential oil raw materials used in this invention are all domestically produced steam distillation methods and are commercially available products that meet relevant national standards. Therefore, this invention does not limit the source of the raw materials. It is worth noting that the manufacturer of the essential oil raw materials in this invention currently does not possess the technical capability to develop essential oils with antibacterial and antiviral properties. This manufacturer only extracts aromatic essential oil raw materials containing volatile components from Chinese herbal medicine raw materials through extraction and distillation. This invention is the result of years of research by our company's traditional Chinese medicine research team, utilizing the pharmacological properties of volatile components in Chinese herbal medicines. This invention will be consistently referred to as "essential oil," and the main component content of the essential oils extracted from the Chinese herbal medicines used in this invention must meet the following standards.

[0036] The main component of peppermint essential oil is menthol (>50%).

[0037] The main components of oregano essential oil are: carvacrol ≥65% and thymol ≥2%.

[0038] Eucalyptus oil (eucalyptus essential oil) main components: eucalyptol ≥85%.

[0039] Chamomile essential oil's main components: sesquiterpenes ≥45%.

[0040] The main components of tea tree essential oil are: r-terpinene ≥22% (r-terpinen ≥22%), 4-terpineol ≥38% (Terpinen-4-ol ≥38%).

[0041] The main components of lemon balm essential oil are: citral ≥ 40% and β-caryophyllene ≥ 25%.

[0042] The main components of thyme essential oil are: thymol ≥ 40%, carvacrol ≥ 5%, and linalool ≥ 35%.

[0043] This invention relates to a combination of essential oils extracted from traditional Chinese medicines. The formula is based on the pharmacological properties of the herbs and utilizes the bactericidal, antibacterial, and antiviral effects of the essential oils. The herbs have a strong, aromatic fragrance, high volatility, and strong penetrating power. They are a direct-contact bactericidal and antibacterial agent with a very strong, all-encompassing bactericidal effect.

[0044] The herbal extracts of this invention can be diluted with any plant base oil to form a formulation, or diluted with alcohol to form a formulation, or the essential oil formulation can be prepared directly, or it can be prepared as a hydrosol formulation. Furthermore, it will be obvious to those skilled in the art that various other variations, substitutions, and modifications can be made without departing from the basic technical concept of this invention, and these modifications still fall within the protection scope of this invention.

[0045] Compared with the prior art, the beneficial effects and advantages of the present invention are as follows:

[0046] The herbal extracts and essential oil combination solution of this invention have a bactericidal rate of >99.97% against Neisseria gonorrhoeae;

[0047] The raw materials in the essential oil composition extracted from traditional Chinese medicine in this invention are edible traditional Chinese medicines, which ensures the safety of this invention in various application scenarios and clinical treatment.

[0048] The essential oil composition preparation extracted from traditional Chinese medicine in this invention is non-irritating to the skin and mucous membranes and has a lubricating effect.

[0049] This invention opens a new chapter in the field of medical technology, which utilizes safe traditional Chinese medicine to effectively kill bacteria and treat infectious diseases. It has opened a new chapter in my country and even the world. Detailed Implementation

[0050] An essential oil composition extracted from traditional Chinese medicine that has a killing effect on Neisseria gonorrhoeae and is suitable for treating urinary tract infections and pharyngitis. It is a medicinal liquid prepared from the following essential oils extracted from traditional Chinese medicine in the following weight ratios: peppermint oil 6-40 parts, oregano oil 12-31 parts, eucalyptus oil 14-80 parts, chamomile oil 10-65 parts, tea tree oil 15-75 parts, lemon balm oil 17-80 parts, thyme oil 9-27 parts, and corn oil (as a base oil) 100-600 parts.

[0051] In this invention, the essential oil combination liquid extracted from traditional Chinese medicine is prepared into a liquid preparation using corn oil as the base oil diluent.

[0052] The peppermint essential oil, by weight, includes, but is not limited to, a point value of any one of 6 to 40 parts or a range between any two.

[0053] The oregano essential oil, by weight, includes, but is not limited to, a point value of any one of 12 to 31 parts or a range between any two.

[0054] The eucalyptus oil, by weight, includes, but is not limited to, a point value or a range between any two of 14 to 80 parts.

[0055] Among them, the chamomile essential oil, by weight, includes but is not limited to the point value of any one of 10 parts to 65 parts or the range between any two.

[0056] Among them, the tea tree essential oil, by weight, includes but is not limited to the point value of any one of 15 to 75 parts or the range between any two.

[0057] The lemon balm essential oil, by weight, includes, but is not limited to, a point value of any one of 17 to 80 parts or a range between any two.

[0058] The thyme essential oil, by weight, includes, but is not limited to, a point value of any one of 9 to 27 parts or a range between any two.

[0059] The corn oil, by weight, includes, but is not limited to, a point value of any one of 100 to 600 parts or a range between any two.

[0060] Example 1: Preparation of a pharmaceutical solution from essential oil compositions extracted from traditional Chinese medicine.

[0061] Formula: 6 parts peppermint essential oil, 12 parts oregano essential oil, 14 parts eucalyptus oil, 10 parts chamomile essential oil, 15 parts tea tree essential oil, 17 parts lemon balm essential oil, 9 parts thyme essential oil, and 300 parts corn oil as a base oil.

[0062] Preparation method:

[0063] ① Weigh the essential oils extracted from the above-mentioned Chinese herbal medicines according to the weight ratio;

[0064] ②Put the mixture into a sealed container at room temperature and stir well;

[0065] ③ Dispense the solution into spray-type packages with a volume of 20ml per bottle;

[0066] ④ Take 200ml of the drug solution and seal it as the original solution sample for the test group to be submitted for testing.

[0067] Example 2: Preparation of a pharmaceutical solution from essential oil compositions extracted from traditional Chinese medicine.

[0068] Formula: 6 parts peppermint essential oil, 12 parts oregano essential oil, 14 parts eucalyptus oil, 10 parts chamomile essential oil, 15 parts tea tree essential oil, 17 parts lemon balm essential oil, 9 parts thyme essential oil, and 310 parts sweet almond oil as a base oil.

[0069] Preparation method:

[0070] ① Weigh the essential oils extracted from the above-mentioned Chinese herbal medicines according to the weight ratio;

[0071] ②Put the mixture into a sealed container at room temperature and stir well;

[0072] ③ Dispense the solution into spray-type packages with a volume of 20ml per bottle;

[0073] ④ Take 200ml of the drug solution and seal it as the original solution sample for the test group to be submitted for testing.

[0074] Example 3: Preparation of a pharmaceutical solution from essential oil compositions extracted from traditional Chinese medicine.

[0075] Formula: 6 parts peppermint essential oil, 12 parts oregano essential oil, 14 parts eucalyptus oil, 10 parts chamomile essential oil, 15 parts tea tree essential oil, 17 parts lemon balm essential oil, 9 parts thyme essential oil, and 280 parts grapeseed oil as a base oil.

[0076] Preparation method:

[0077] ① Weigh the essential oils extracted from the above-mentioned Chinese herbal medicines according to the weight ratio;

[0078] ②Put the mixture into a sealed container at room temperature and stir well;

[0079] ③ Dispense the solution into spray-type packages with a volume of 20ml per bottle;

[0080] ④ Take 200ml of the drug solution and seal it as the original solution sample for the test group to be submitted for testing.

[0081] The difference between the herbal extract essential oil compositions in Examples 1, 2, and 3 is that they each use different base oils as diluents.

[0082] The preparations obtained in Examples 1, 2, and 3 are uniformly named "Wei Ai Shu Gong Yin Jie".

[0083] With the support of the collaborating unit, the Guangdong Institute of Microbiology / Guangdong Provincial Microbiology Analysis and Testing Center (a national P3-level microbiology laboratory), 200ml samples of the original solutions from each of the reagents prepared in Examples 1, 2, and 3 were extracted for laboratory analysis and testing.

[0084] The drug stock solution samples prepared in each embodiment were used as the stock solutions for the test group for detection.

[0085] The 200ml packaged test group stock solution sample of the formulation in Example 1 above participated in 1 test.

[0086] The 200ml packaged test group stock solution sample of the formulation in Example 2 above participated in 2 tests;

[0087] The 200ml packaged test group original solution sample of the formulation in Example 3 above participated in the test 3 times.

[0088] The testing was conducted in accordance with the national health industry standard: "WS / T 650-2019 Evaluation Method for Antibacterial and Bacteriostatic Effects", and the testing unit was the Guangdong Institute of Microbiology / Guangdong Provincial Microbiology Analysis and Testing Center, a national P3-level microbiology laboratory.

[0089] Testing standards and methods: WS / T 650-2019 (5.2.1)

[0090] Test item: Quantitative sterilization test of suspension

[0091] Test Report Number (Report N) o ): 2025SP04517R01

[0092] Verification Code for Test Result Inquiry: XJ4HVBF4

[0093] Evaluation of test results: The herbal extract essential oil composition of the present invention has a bactericidal rate of >99.97% against Neisseria gonorrhoeae, as detailed in Table 1.

[0094] Table 1. Results of bactericidal analysis and detection of Neisseria gonorrhoeae.

[0095] Report Number: (Report N) o.) 2025SP04517R01 Verification Code: XJ4HVBF4

[0096]

[0097] As can be seen from Table 1, the essential oil composition extracted from traditional Chinese medicine in this invention has a strong bactericidal rate against Neisseria gonorrhoeae.

[0098] Clinical application effect observation of the essential oil composition extracted from Chinese herbal medicines in this invention

[0099] From May 2023 to August 2024, in cooperation with several hospitals in the province, we recruited 40 patients infected with Neisseria gonorrhoeae (20 men and 20 women), 40 women with bacterial vaginosis (BV), and 40 women with chronic pharyngitis, for a total of 120 volunteer patients to conduct clinical efficacy observations for this example. All expenses for the volunteer patients were borne by our company.

[0100] Of the 120 volunteer patients in this example, 40 were male and 20 were female, forming the clinical efficacy study group of Example 1.

[0101] Forty female patients with bacterial vaginosis (BV) among the 120 volunteer patients in Example 2 were included in the clinical efficacy study group of Example 2.

[0102] Forty male and female patients with chronic pharyngitis among the 120 volunteer patients in the example were used as the clinical effect research group in Example 3.

[0103] Inclusion criteria for volunteer patients in the research group of Example 1;

[0104] (1) Meeting the medical detection diagnosis of Neisseria gonorrhoeae infection in the urogenital system;

[0105] (2) No tumor mass detected by imaging diagnosis;

[0106] (3) Aged 22 to 55 years old;

[0107] (4) Without diabetes symptoms;

[0108] (5) Without kidney disease symptoms;

[0109] (6) Meeting the follow-up conditions;

[0110] (7) Signing a privacy confidentiality agreement.

[0111] In the research group of volunteer patients in Example 1, there were 20 male and 20 female patients. Among them, the age of male patients was 34 to 55 years old, with an average age of (41.3 ± 4.6) years; the age of female patients was 22 to 53 years old, with an average age of (31 ± 5.1) years. Both male and female patients met the medical diagnosis of Neisseria gonorrhoeae infection in the urogenital system. For data such as patient gender, age, education level, and disease course, SPSS 20.0 software was used to analyze the data, and there were no significant differences in the general information of volunteer patients (P > 0.05).

[0112] Explanation of clinical drug treatment and contraindication suggestions in the research group of Example 1: The external spray "Wei'ai Shu Gongyin Jie" of the medicament in Example 1 was used, spraying the medicament three times a day in the morning, middle, and evening, with about 3 - 7 milliliters each time; 7 days was a medication course, stopping the drug for 15 days for the first reexamination after 7 days, and then having the second reexamination after another 15 days; when male patients used the drug, the liquid medicine was sprayed on the glans and anus, and pay attention to spraying some liquid medicine into the urethral orifice of the glans; when female patients used the drug, it was sprayed on the vulva, inner vagina, deep vagina, and anus, and also try to spray some liquid medicine into the urethral orifice. During the medication period, spicy foods are prohibited, and sexual intercourse is prohibited; daily personal items should be sterilized and cleaned every day. It is recommended to use disposable sanitary underwear during the treatment period, and it is recommended to shave the pubic hair clean.

[0113] Clinical efficacy evaluation: The negative and positive results of two follow-up medical tests for Neisseria gonorrhoeae are used to calculate the Neisseria gonorrhoeae seroconversion rate; if two follow-up medical tests for Neisseria gonorrhoeae are negative and clinical symptoms have disappeared, it is evaluated as clinical cure; if two follow-up medical tests for Neisseria gonorrhoeae are negative but some clinical symptoms have not disappeared, it is evaluated as clinical effectiveness; if the first medical test for Neisseria gonorrhoeae is negative and the second test is positive, the clinical ineffectiveness rate is calculated.

[0114] Example 1 Clinical efficacy study group: After 7 days of medication and 15 days of drug withdrawal, the first follow-up examination and the second follow-up examination 15 days later showed that the gonococcal conversion rate was 100% and the clinical cure rate reached 95%, as shown in Table 2.

[0115] Table 2. Clinical data of the study group in Example 1 after 7 days of treatment for Neisseria gonorrhoeae infection and 15 days after drug withdrawal.

[0116]

[0117] As shown in Table 2, the clinical efficacy of the 40 volunteer patients in the Example 1 study group showed that the negative conversion rate of Neisseria gonorrhoeae in two follow-up examinations was 100%. This indicates that the "Wei Ai Shu Gong Yin Jie" medication has an ideal clinical effect on urogenital infections caused by Neisseria gonorrhoeae.

[0118] Example 2: Inclusion criteria for volunteer patients in the study group;

[0119] (1) Meets the criteria for a medical diagnosis of bacterial vaginosis (BV);

[0120] (2) Imaging diagnosis showed no tumor mass;

[0121] (3) Age between 22 and 50 years old;

[0122] (4) No symptoms of diabetes;

[0123] (5) No symptoms of kidney disease;

[0124] (6) Conditions for follow-up visits are available;

[0125] (7) Sign a privacy and confidentiality agreement.

[0126] Example 2: The study group included 40 female volunteers, aged 22–42 years, with a mean age of (29.3±4.2) years. All patients met the medical diagnosis of bacterial vaginosis (BV) of the urogenital system. Data on patient gender, age, education level, and disease duration were analyzed using SPSS 20.0 software. There were no significant differences in general characteristics among the volunteer patients (P>0.05).

[0127] Bacterial vaginosis (BV), also known as bacterial vaginosis, often presents with no obvious clinical symptoms. Some patients may experience vaginal discomfort or burning sensation. The main symptoms include an unpleasant odor, which becomes more pronounced during sexual intercourse, increased vaginal discharge, thin and pasty discharge, grayish-white or grayish-green discharge with an abnormal odor, and a vaginal pH value greater than 4.5. In severe cases, it can lead to cervicitis, uterine effusion, pelvic inflammatory disease, endometritis, or other urogenital system diseases.

[0128] The diagnosis of bacterial vaginosis mainly refers to the Amsel clinical diagnostic criteria: meeting at least three of the following four criteria: grayish-white homogeneous discharge (leukorrhea), vaginal pH > 4.5, positive amine test (the discharge releases a fishy odor after reacting with potassium hydroxide), and clue cells ≥ 20% (microscopic observation of a large number of bacteria attached to the surface of exfoliated vaginal epithelial cells). In addition to these four diagnostic tests, examinations are generally performed to rule out sexually transmitted infections, trichomoniasis, and yeast infections, as well as a vaginal cleanliness examination. After ruling out sexually transmitted infections, trichomoniasis, and yeast infections, the severity of bacterial vaginosis is confirmed by assessing vaginal cleanliness levels between II, III, and IV.

[0129] Example 2 Clinical efficacy evaluation of the study group was based on the Amsel clinical diagnostic criteria: normal vaginal discharge color and no odor, vaginal pH ≤ 4.5, negative amine test, clue cells < 20%, and vaginal cleanliness between I and II.

[0130] Clinical efficacy evaluation: If the vaginal discharge is normal in color and odorless, the vaginal pH is ≤4.5, the amine test is negative, the clue cells are <20%, and the vaginal cleanliness is between I and II, then it is evaluated as clinically cured; if any one of these four criteria is not met, or if only the vaginal cleanliness is between II and III, then it is evaluated as clinically effective; if there is no significant change in symptoms, it is evaluated as clinically ineffective.

[0131] Clinical medication treatment and contraindications recommendations for the study group in Example 2: Use the external spray "Wei Ai Shu Gong Yin Jie" from Example 2, spraying the medication three times a day (morning, noon, and evening), about 3-7 ml each time; 7 days constitutes one course of treatment, and after 7 days, stop medication for 15 days and then have a follow-up examination; spray the medication on the vulva, vagina, and deep inside the vagina. Avoid spicy food and sexual intercourse during the treatment period; personal items should be cleaned daily, and it is recommended to wear disposable sanitary underwear during the treatment period.

[0132] Example 2 Clinical efficacy of the study group: After 7 days of medication and 15 days of drug withdrawal, the clinical cure rate reached 97.5% and the clinical effective rate was 100%, as shown in Table 3.

[0133] Table 3. Clinical data of the study group in Example 2 who underwent 7 days of treatment for bacterial vaginosis and were followed up 15 days after discontinuation of medication.

[0134]

[0135] As shown in Table 3, the clinical efficacy rate of the 40 volunteer patients in Example 2 study group was 100%, and the clinical cure rate was 97.5%. This indicates that the "Wei Ai Shu Gong Yin Jie" medication has good clinical efficacy against bacterial vaginosis (BV).

[0136] Example 3: Inclusion criteria for volunteer patients in the study group;

[0137] (1) Meets the criteria for a medical diagnosis of chronic pharyngitis;

[0138] (2) Imaging diagnosis showed no tumor mass;

[0139] (3) Those aged 22 to 65;

[0140] (4) No symptoms of diabetes;

[0141] (5) No symptoms of kidney disease;

[0142] (6) Conditions for follow-up visits are available;

[0143] (7) Sign a privacy and confidentiality agreement.

[0144] Example 3: Inclusion and exclusion criteria for volunteer patients in the study group;

[0145] (1) Patients with pharyngeal tumors;

[0146] (2) Patients with existing lymphoid follicle hyperplasia

[0147] (3) Patients with chronic atrophic pharyngitis;

[0148] (4) Patients with thyroid disorders;

[0149] (5) Patients with gastroreflux pharyngitis;

[0150] (6) Patients with sinusitis;

[0151] (7) Those who have had throat surgery.

[0152] Example 3: The study group consisted of 29 male and 11 female volunteers. Males ranged in age from 32 to 62 years (mean 43.1 ± 5.3 years), while females ranged in age from 22 to 57 years (mean 39.4 ± 5.4 years). All patients met the medical diagnosis of chronic pharyngitis. Data on patient gender, age, education level, and disease duration were analyzed using SPSS 20.0 software. No significant differences were found in the general characteristics of the volunteer patients (P > 0.05).

[0153] Chronic pharyngitis is mostly caused by bacterial inflammation. If left untreated for a long time, it can lead to organic lesions such as lymphoid follicle hyperplasia. In the early stages of the disease, many practitioners of traditional Chinese medicine easily confuse it with the condition of "plum pit qi" (a traditional Chinese medicine term for globus pharyngitis). While "plum pit qi" is often caused by emotional distress, if it is not treated in time, it can also develop into chronic pharyngitis as it is known in Western medicine.

[0154] The main symptoms of chronic pharyngitis include a foreign body sensation, phlegm blockage, dry and itchy throat, redness and swelling in the throat, mild pain, and burning sensation in the throat. However, the more obvious symptoms such as foreign body sensation, phlegm blockage, and dry and itchy throat are often present for a long time.

[0155] Clinical medication treatment and contraindications recommendations for the study group in Example 3: The medication "Wei Ai Shu Gong Yin Jie" spray from Example 3 was used. The spray was applied three times daily (morning, noon, and evening), approximately 3-7 ml each time, for a 7-day course of treatment. After oral spraying, the spray should be held in the mouth for about 15 seconds to allow the aroma to penetrate the throat. Swallow or spit it out after feeling the aroma in the stomach. "Wei Ai Shu Gong Yin Jie" is edible; do not rinse your mouth or eat within 30 minutes after application. A follow-up examination should be conducted 15 days after stopping medication. Avoid spicy foods during treatment. Disposable toothbrushes are recommended. Shared meals are prohibited during treatment; when dining together, separate serving utensils or serving chopsticks should be used.

[0156] The clinical efficacy evaluation of chronic pharyngitis is mainly based on the disappearance of clinical symptoms. Complete disappearance of clinical symptoms is evaluated as clinical cure; partial disappearance of clinical symptoms is evaluated as clinical effectiveness; and the continued presence of various clinical symptoms is evaluated as clinical ineffectiveness. Example 3: The clinical efficacy study group, after 7 days of medication followed by a 15-day re-examination, showed a clinical cure rate of 97.5%, as shown in Table 4.

[0157] Table 4. Clinical efficacy of the study group in Example 3 after 7 days of treatment for chronic pharyngitis and 15 days of drug withdrawal.

[0158]

[0159] As shown in Table 4, the clinical efficacy rate of the 40 volunteer patients in Example 3 was 100%, and the clinical cure rate was 97.5%. This indicates that "Wei Ai Shu Gong Yin Jie" has a good clinical effect on chronic pharyngitis.

[0160] In summary, the herbal extract essential oil composition provided by this invention has the effect of killing Neisseria gonorrhoeae and is suitable for treating urinary tract infections and pharyngitis. The medicine of this invention is non-irritating to the skin and mucous membranes, has high patient compliance, rapid clinical onset and short treatment course. In the context of sexual intercourse, in addition to its lubricating effect, it is also conducive to the prevention and control of sexually transmitted infections. This invention has significance in terms of national public health and medical interests and can be widely used in the fields of medicine, health care products, antibacterial and bacteriostatic hygiene products.

[0161] The embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments. Based on the above description of the present invention, and according to ordinary technical knowledge and common practice in the art, it will be obvious to those skilled in the art that various other changes and modifications can be made to these embodiments without departing from the basic technical concept of the present invention, and these changes still fall within the protection scope of the present invention.

Claims

1. A Chinese herbal medicine extracted essential oil composition having a killing effect on Neisseria gonorrhoeae, suitable for treating urogenital system infection and inflammation of the throat, prepared from a medicinal liquid containing essential oils of Chinese herbal medicines in the following proportions by weight: peppermint essential oil 6-40 parts, oregano essential oil 12-31 parts, eucalyptus oil (also known as eucalyptus essential oil, blue press leaf oil) 14-80 parts, chamomile essential oil 10-65 parts, tea tree essential oil (also known as Australian tea tree essential oil, melaleuca alternifolia leaf essential oil, melaleuca alternifolia leaf essential oil) 15-75 parts, balm essential oil 17-80 parts, and thyme essential oil 9-27 parts.

2. The Chinese herbal essential oil combination liquid of claim 1, wherein the Chinese herbal essential oil combination liquid is a liquid extract of a Chinese herbal medicine. Prepared from essential oils of Chinese herbal medicines including peppermint essential oil, oregano essential oil, eucalyptus oil, chamomile essential oil, tea tree essential oil, balm essential oil, and thyme essential oil.

3. Essential oils in the Chinese herbal medicine essential oil combination medicinal liquid according to claims 1-2, which include Chinese herbal medicine essential oils (also known as plant essential oils, herbal essential oils), and preparations prepared by adding pharmaceutically and food acceptable excipients; and also include medicines combined with the main components extracted from the Chinese herbal medicine essential oils according to claims 1-2, and preparations prepared by adding pharmaceutically and food acceptable excipients.

4. The Chinese herbal medicine essential oil combination liquid of claims 1 to 3, characterized by It is a preparation that can be used directly; further, the Chinese herbal medicine essential oil combination medicinal liquid added with pharmaceutically and food acceptable excipients or auxiliary ingredients includes various aroma essential oils, perfumes, fragrances, taste agents, solubilizing and liquefying agents, and various base oils or diluents to prepare various preparations.

5. The Chinese herbal medicine essential oil combination liquid and the preparation according to claims 1 to 4, characterized in that It can be in the form of a spray, gargle, push, drop, liquid, oil, emulsion, ointment, suppository, jelly, soft candy, wet paper, and other media release forms.

6. The Chinese herbal medicine essential oil combination medicinal liquid and the preparation according to any one of claims 1-5, which includes the use in the preparation of medicines for Neisseria gonorrhoeae infection or male and female urogenital system infection and inflammation of the throat.

7. The Chinese herbal medicine essential oil combination medicinal liquid and the preparation according to any one of claims 1-5, which includes the use in the preparation of medicines for the esophagus and digestive system.

8. The Chinese herbal medicine essential oil combination medicinal liquid and the preparation according to any one of claims 1-5, which includes the use in the preparation of medicines, drugs, health products, food and beverages, medical disinfectants, health products, and other products with bactericidal, antibacterial, and bacteriostatic functions.

9. The Chinese herbal medicine essential oil combination according to any one of claims 1 to 5, wherein: the Chinese herbal medicine essential oil combination is characterized by In the protection period after the publication date of the Chinese application of the present application, the organization, unit, team or individual research and development of the preparation has the same raw material combination formula as any 2-6 kinds of essential oils in any one of claims 1-2, for preparing the Chinese herbal medicine essential oil combination liquid or the preparation according to any one of claims 1-8; or its feature is that in the protection period after the publication date of the Chinese application of the present application, the organization, unit, team or individual research and development of the preparation has the same main component extraction of any 2-6 kinds of essential oils in any one of claims 1-2, for preparing the preparation according to any one of claims 1-8.

10. The Chinese herbal medicine essential oil combination liquid and the preparation according to any one of claims 1 to 5, characterized in that, The skilled in the art will process the above-mentioned Chinese herbal medicine essential oil combination liquid or through independent processing, by adding excipients, base liquid, base oil, diluent, by adding essential oils, essences, taste agents for adjusting the aroma type in preparing the Chinese herbal medicine essential oil combination liquid or the preparation according to any one of claims 1-8.

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