Traditional Chinese medicine composition for inhibiting helicobacter pylori and mouth wash
By preparing a mouthwash containing specific plant extracts, the problem of unsatisfactory effects of existing Helicobacter pylori inhibitory products has been solved, achieving highly effective antibacterial and oral hygiene protection without side effects.
Patent Information
- Application Number
- CN202410870880.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2026-01-06
AI Technical Summary
Existing oral care products that inhibit Helicobacter pylori are not very effective and may have side effects.
A mouthwash is prepared by using a combination of green tea extract, honeysuckle extract, dandelion extract, dried plum extract, clove extract and scutellaria baicalensis extract, along with a humectant, sweetener, preservative, acidulant, cooling agent, solubilizer and antibacterial agent.
It effectively inhibits Helicobacter pylori, has a good taste, no side effects, protects oral hygiene, and prevents Helicobacter pylori infection.
Smart Images
Figure CN121265699A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of oral care product technology, specifically to a traditional Chinese medicine composition and mouthwash for inhibiting Helicobacter pylori. Background Technology
[0002] Helicobacter pylori (HP) is a microaerophilic, Gram-negative bacterium that can colonize areas such as the oral cavity and gastric mucosa. It is an infectious pathogen and a major contributing factor to infectious halitosis, oral ulcers, chronic active gastritis, peptic ulcers, and gastric cancer. In 2017, the World Health Organization classified Helicobacter pylori as a Group 1 carcinogen.
[0003] As the entrance to the digestive tract, the presence of Helicobacter pylori in the oral cavity affects the efficacy of Helicobacter pylori eradication therapy. Maintaining good oral hygiene and preventing oral infections are increasingly becoming important measures for preventing Helicobacter pylori infection, halitosis, and stomach problems. Oral products that inhibit Helicobacter pylori can reduce the probability of Helicobacter pylori entering and colonizing the stomach, thus reducing its pathogenicity and improving the efficacy of eradication therapy. Although some products that inhibit Helicobacter pylori exist, such as mouthwash, toothpaste, and oral sprays, their antibacterial effects are not ideal.
[0004] Therefore, how to provide an oral product that can effectively inhibit Helicobacter pylori has become a problem that needs to be solved. Summary of the Invention
[0005] To address the aforementioned problems, the present invention aims to provide a traditional Chinese medicine composition and mouthwash for inhibiting Helicobacter pylori. This traditional Chinese medicine composition exhibits excellent inhibitory effects on Helicobacter pylori, and its components are all plant extracts, making the raw materials green and healthy. Adding it to mouthwash not only effectively inhibits Helicobacter pylori but also does not produce side effects such as affecting taste or odor.
[0006] To achieve the above objectives, the specific technical solution adopted by the present invention is as follows:
[0007] A first aspect of the present invention is to provide a traditional Chinese medicine composition for inhibiting Helicobacter pylori, comprising green tea extract, honeysuckle extract, dandelion extract, dried plum extract, clove extract and scutellaria extract.
[0008] The innovation of this invention lies in the discovery, through screening combinations of different plant extracts, that the combination of the above six plant extracts exhibits a significant inhibitory advantage against Helicobacter pylori.
[0009] A second aspect of the present invention is to provide the application of the above-mentioned traditional Chinese medicine composition in the preparation of oral products that inhibit Helicobacter pylori. Specifically, a mouthwash for inhibiting Helicobacter pylori is provided, comprising plant-based active ingredients and excipients, wherein the plant-based active ingredients are the traditional Chinese medicine composition for inhibiting Helicobacter pylori.
[0010] Furthermore, the excipients include wetting agents, sweeteners, preservatives, acidulants, cooling agents, solubilizers, and antibacterial agents. The wetting agents include sorbitol and propylene glycol; the sweeteners include xylitol and stevia; the preservative is sodium benzoate; the acidulant is citric acid; the cooling agent is menthol; the solubilizer is poloxamer-407; and the antibacterial agent is domiphen (which has inhibitory effects on Staphylococcus aureus, Escherichia coli, and Candida albicans).
[0011] Furthermore, the components of the mouthwash, by weight, include: 1-20 parts green tea extract, 1-20 parts honeysuckle extract, 1-10 parts dandelion extract, 1-10 parts dried plum extract, 1-10 parts clove extract, 1-80 parts scutellaria baicalensis extract, 50-100 parts sorbitol, 20-90 parts propylene glycol, 1-5 parts stevia, 40-100 parts xylitol, 1-10 parts citric acid, 1-10 parts menthol, 1-10 parts sodium benzoate, 1-70 parts poloxamer-407, and 1-10 parts domiphen.
[0012] Furthermore, the mouthwash that inhibits Helicobacter pylori is prepared by the following method:
[0013] (1) Add the solubilizer to deionized water and stir until well mixed, then add the cooling agent and stir until well mixed;
[0014] (2) While stirring, add the plant active ingredients, wetting agent, sweetener, acidulant and preservative to the solution obtained in step (1) and stir until uniform;
[0015] (3) Adjust the pH of the solution to 5.5-6.5 with a pH adjuster, then add the antibacterial agent and mix well;
[0016] (4) Stop stirring, filter, and sterilize to obtain the mouthwash product.
[0017] Furthermore, in step (3) of the preparation method, the pH adjuster is disodium hydrogen phosphate.
[0018] The present invention has the following beneficial effects:
[0019] 1. This invention proposes a traditional Chinese medicine composition composed of plant extracts that can effectively inhibit Helicobacter pylori and has been successfully applied to the preparation of mouthwash.
[0020] 2. The mouthwash of this invention is highly targeted and easy to use. It has the functions of cleaning, antibacterial and freshening breath, and has no toxic side effects.
[0021] 3. This mouthwash is made from all-natural and healthy ingredients. Its unique and carefully formulated recipe and simple manufacturing method have been proven to have a strong inhibitory effect on Helicobacter pylori, protecting oral hygiene and preventing the spread of Helicobacter pylori.
[0022] 4. The mouthwash of this invention has a pleasant taste, no unpleasant odor, and is easily accepted by a wide range of people. Attached Figure Description
[0023] Figure 1 Experimental data from the 8-factor, 4-level orthogonal experiment in Example 1. Detailed Implementation
[0024] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0025] Example 1
[0026] This embodiment provides a traditional Chinese medicine composition for inhibiting Helicobacter pylori, including green tea extract, honeysuckle extract, dandelion extract, dried plum extract, clove extract, and scutellaria baicalensis extract.
[0027] The screening method for this traditional Chinese medicine composition is as follows: Based on previous experiments, the MBC (minimum bactericidal concentration) of each extract was determined. Then, an 8-factor, 4-level orthogonal experiment was conducted at 2-4 times the MBC to select the group with the most prominent antibacterial effect. The specific process is as follows:
[0028] I. Raw Material Preparation
[0029] Prepare the following 8 plant extracts: green tea extract, honeysuckle extract, dandelion extract, coptis extract, dried plum extract, clove extract, licorice extract, and scutellaria extract.
[0030] The specific sources of the raw materials used in this embodiment are as follows: Green tea extract was purchased from Zhejiang Shengtong Biotechnology Co., Ltd., batch number 221210E; Honeysuckle extract, dandelion extract, Coptis chinensis extract, dried plum extract, clove extract, licorice extract, and Scutellaria baicalensis extract were all purchased from Baoji Xuhuang Biotechnology Co., Ltd., batch numbers 220801, 220701, 220901, 220901, 221001, 221001, and 220901, respectively.
[0031] II. Antibacterial Test
[0032] Prepare the raw materials according to Figure 1The raw materials were mixed according to the combination method and the amount (parts by weight) of the additives, using deionized water as the solvent, to prepare 32 sets of experimental samples. The experimental samples were sterilized at 121℃ for 20 minutes. After sterilization, the samples were placed in a biosafety cabinet through a transfer window. 50 μL of Helicobacter pylori bacterial suspension was spread onto HP medium. Four 6 mm circular paper discs were evenly placed on the medium, and 10 μL of sample was dropped onto each disc for diffusion. After the operation, the medium was placed in an incubator for cultivation. The size of the inhibition zone on the medium was observed and measured; a larger inhibition zone indicated a better antibacterial effect.
[0033] Test results are as follows Figure 1 As shown, the 16th group of experimental samples has the largest inhibition zone and the best inhibitory effect on Helicobacter pylori. Its composition is: green tea extract, honeysuckle extract, dandelion extract, dried plum extract, clove extract, and scutellaria baicalensis extract (without licorice and coptis extracts).
[0034] Therefore, these six extracts were selected as the traditional Chinese medicine composition of this invention. Besides exhibiting anti-Helicobacter pylori effects when combined, each of the six extracts also possesses the following individual effects, making them superior raw materials for mouthwash.
[0035] The green tea extract has the effects of clearing the head and eyes, relieving thirst, aiding digestion, resolving phlegm, promoting urination, and detoxifying. Clinically, it has a therapeutic effect on dental plaque and gingival bleeding.
[0036] The honeysuckle extract has the effects of clearing heat and detoxifying, reducing inflammation and swelling. Clinically, it has a therapeutic effect on oral ulcers, tooth decay, and gingivitis, and can also inhibit halitosis.
[0037] The dandelion extract has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and promoting diuresis and relieving strangury. In addition, dandelion has antibacterial and anti-inflammatory effects and can treat oral ulcers.
[0038] The extract of dried plum has the effects of astringing the lungs, astringing the intestines, promoting the production of body fluids, and expelling roundworms. Clinically, it has a therapeutic effect on oral ulcers.
[0039] The clove extract has the effects of warming the middle jiao and relieving nausea, tonifying the kidney and assisting yang, and has a therapeutic effect on toothache and oral ulcers in clinical practice.
[0040] The Scutellaria baicalensis extract has the effects of clearing heat and drying dampness, purging fire and detoxifying, stopping bleeding, and calming the fetus. Scutellaria baicalensis has a broad antibacterial spectrum and has an antagonistic effect on bacteria in the oral cavity. It has therapeutic effects on tooth decay, periodontal disease, tooth sensitivity, halitosis, and oral ulcers.
[0041] Example 2
[0042] This invention provides a mouthwash that inhibits Helicobacter pylori, the composition of which is shown in the table below. This embodiment provides some specific examples of this mouthwash (see groups 1-3 in the table below).
[0043]
[0044]
[0045] Weigh the raw materials according to the dosage (parts by weight) in the table above, and prepare the mouthwash for groups 1-3 according to the following steps:
[0046] (1) Add the solubilizer to deionized water and stir until well mixed, then add the cooling agent and stir until well mixed;
[0047] (2) While stirring, add the plant active ingredients, wetting agent, sweetener, acidulant and preservative to the solution obtained in step (1) and stir until uniform;
[0048] (3) Adjust the pH of the solution to about 6 with a pH adjuster, then add the antibacterial agent and mix well;
[0049] (4) Stop stirring, filter, and sterilize to obtain the mouthwash product.
[0050] Note: The solubilizer dissolves slowly and needs to be prepared in advance before use; oil-soluble components need to be dispersed in water in advance, as adding the solubilizer later will affect the dispersion of the oil-soluble components.
[0051] Mouthwash antibacterial test:
[0052] Mouthwash samples from groups 1-3 were used as experimental samples. The samples were sterilized at 121℃ for 20 minutes. After sterilization, the samples were transferred to a biosafety cabinet via a pass-through window. A bacterial suspension with a concentration of 1×10⁸ CFU / mL was prepared. In sterile tubes for the detoxification test, 0.5 mL of Helicobacter pylori suspension was added, followed by 0.5 mL of 3% BSA solution. The mixture was mixed and allowed to stand at room temperature for 5 minutes. Then, 4.0 mL of the sample suspension was injected into the solution using a sterile pipette and quickly mixed. After the two solutions interacted, 0.5 mL of the Helicobacter pylori and sample mixture was added to 4.5 mL of 3% BSA solution, mixed, and allowed to stand for 10 minutes. 1.0 mL of the sample solution was then inoculated onto culture media, spreading evenly. Two plates were inoculated from each sample solution. All experimental samples were incubated at 37℃. The number of viable bacteria was determined and counted using the viable bacteria culture counting method.
[0053] The results showed that the mouthwashes in groups 1-3 all achieved an inhibition rate of 99.99% against Helicobacter pylori, demonstrating a significant antibacterial effect.
[0054] This specific embodiment is merely an explanation of the present invention and is not intended to limit the present invention. Any changes made by those skilled in the art after reading the specification of the present invention, as long as they are within the scope of the claims of the present invention, will be protected by patent law.
Claims
1. A traditional Chinese medicine composition for inhibiting Helicobacter pylori, characterized in that, The green tea extract, honeysuckle extract, dandelion extract, dark plum extract, clove extract and skullcap extract.
2. A mouthwash for inhibiting Helicobacter pylori, characterized by comprising, The plant effective ingredient is the traditional Chinese medicine composition for inhibiting Helicobacter pylori in claim 1.
3. The mouthwash for inhibiting H. pylori according to claim 2, characterized by, The auxiliary materials include wetting agent, sweetener, preservative, acidulant, cooling agent, solubilizer and bacteriostatic agent.
4. The mouthwash for inhibiting H. pylori according to claim 3, characterized by, The wetting agent includes sorbitol and propylene glycol, the sweetener includes xylitol and stevia, the preservative is sodium benzoate, the acidulant is citric acid, the cooling agent is menthol, the solubilizer is poloxamer-407 and the bacteriostatic agent is domiphen bromide.
5. The mouthwash for inhibiting H. pylori according to claim 4, characterized by, Each component includes, in terms of mass parts, green tea extract 1-20 parts, honeysuckle extract 1-20 parts, dandelion extract 1-10 parts, dark plum extract 1-10 parts, clove extract 1-10 parts, skullcap extract 1-80 parts, sorbitol 50-100 parts, propylene glycol 20-90 parts, stevia 1-5 parts, xylitol 40-100 parts, citric acid 1-10 parts, menthol 1-10 parts, sodium benzoate 1-10 parts, poloxamer-407 1-70 parts and domiphen bromide 1-10 parts.
6. The mouthwash for inhibiting H. pylori according to any one of claims 3 to 5, characterized in that, The preparation method of the mouthwash includes the following steps: (1) adding the solubilizer into deionized water and stirring until uniform, and then adding the cooling agent and stirring until uniform; (2) under stirring, adding the plant effective ingredient, wetting agent, sweetener, acidulant and preservative into the solution obtained in step (1) and stirring until uniform; (3) adjusting the pH of the solution to 5.5-6.5 with a pH regulator, and then adding the bacteriostatic agent and mixing until uniform; (4) stopping stirring, filtering and sterilizing to obtain the mouthwash product.
7. The mouthwash for inhibiting H. pylori according to claim 6, characterized by, In step (3) of the preparation method, the pH regulator is disodium hydrogen phosphate.