A tumor chemotherapy drug sensitivity detection kit and detection method

Abstract

The present application relates to the technical field of tumor chemotherapy drug sensitivity detection, and discloses a tumor chemotherapy drug sensitivity detection kit and a detection method, which comprise a box body, two groups of driving mechanisms and two groups of equal medicine adding mechanisms, further comprise a box cover installed on one side of the box body through a hinge, and six groups of sample injectors arranged in an annular array at the bottom of the equal medicine adding mechanisms; the technical scheme of the present application is characterized by gear transmission assemblies, spline transmission assemblies and bevel gear linkage assemblies; the gear transmission assemblies drive the sample injectors to advance into the test tubes by rotating and driving the rack-and-pinion lifting frame to move downward, thus achieving the functions of accurately controlling the injection speed and stroke, ensuring the equal amount of injected chemotherapy drugs or lysis liquid in each test tube, realizing the equal control of the medicine adding or liquid adding amount of the six groups of detection samples, ensuring the consistency of the reaction conditions, and improving the reliability of the tumor chemotherapy drug sensitivity detection results.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tumor chemotherapy drug sensitivity detection, and in particular to a tumor chemotherapy drug sensitivity detection kit and a detection method. BACKGROUND

[0002] Tumor chemotherapy is a commonly used cancer treatment method, which kills rapidly dividing cancer cells by using chemotherapy drugs; however, different patients have great differences in response to chemotherapy drugs, which directly affects the treatment effect and the quality of life of patients; in order to improve the treatment effect, scientists have developed a series of methods and kits for detecting the sensitivity of tumors to chemotherapy drugs, aiming to predict the patient's response to a specific chemotherapy drug by detecting individual differences in advance, so as to select the most suitable treatment plan for the patient; the development of such methods and kits is an important part of personalized medicine, which provides a basis for doctors to make decisions and helps to optimize treatment strategies and improve treatment effect;

[0003] In the process of implementing the present application, the inventors found that at least the following problems exist in the prior art:

[0004] The existing tumor chemotherapy drug sensitivity detection kit cannot achieve precise control of the drug amount of multiple samples; when detecting samples, the same amount of chemotherapy drugs or lysis solution needs to be added to multiple samples to start the reaction; due to the unequal control of the injection amount of multiple samples, the inconsistency of the reaction conditions of multiple samples is caused, which directly affects the accuracy of the tumor chemotherapy drug sensitivity detection result; thereby affecting the accurate evaluation of the sensitivity of tumor cells to a specific chemotherapy drug, leading to the problem of incorrect selection of treatment plan and affecting the treatment effect of patients.

[0005] Therefore, the above technical problems need to be solved. SUMMARY

[0006] In order to overcome the shortcomings of the prior art, the present application provides a tumor chemotherapy drug sensitivity detection kit and a detection method to solve the problem that precise control of the drug amount of multiple samples cannot be achieved, resulting in inconsistency of the reaction conditions of multiple samples, which directly affects the accuracy of the tumor chemotherapy drug sensitivity detection result.

[0007] In order to solve the above technical problems, the basic technical scheme of the present application is as follows:

[0008] The application discloses a tumor chemotherapy drug sensitivity detection kit, which comprises a box body, two groups of driving mechanisms and two groups of equal-dose medicine adding mechanisms, a box cover which is installed on one side of the box body through a hinge, and six groups of sample injectors which are arranged in an annular array on the bottom of the equal-dose medicine adding mechanisms.

[0009] A power transmission mechanism is rotatably installed at the center of the bottom of the equal-dose medicine adding mechanism, and the power transmission mechanism comprises a linkage bevel gear and six groups of liquid-stopping telescopic components; the six groups of liquid-stopping telescopic components are dynamically meshed with the linkage bevel gear, and one end of each of the six groups of liquid-stopping telescopic components extends into the inside of the six groups of mounting sleeves.

[0010] The two groups of driving mechanisms are respectively embedded and installed in the inside of the box cover, and the two groups of equal-dose medicine adding mechanisms are fixedly installed at the bottom of the two groups of driving mechanisms; the driving mechanism comprises a protective cover, a dovetail concave frame, a driving telescopic frame, a rack lifting frame, a gear transmission assembly and a spline transmission assembly; the dovetail concave frame and the driving telescopic frame are fixedly installed on the top wall in the inside of the protective cover; the rack lifting frame is movably installed in the inside of the dovetail concave frame, and the bottom of the rack lifting frame is fixedly connected with the bottom of the driving telescopic frame; the gear transmission assembly is rotatably installed in the inside of the driving telescopic frame, and the gear transmission assembly is meshedly connected with the rack lifting frame; the spline transmission assembly is installed on one side of the driving telescopic frame through a shaft support, and one end of the gear transmission assembly is meshedly connected with the bottom of the spline transmission assembly.

[0011] The equal-dose medicine adding mechanism comprises a storage tank, a bevel gear linkage assembly and a pressing medicine adding assembly; the bevel gear linkage assembly is fixedly installed on the top of the storage tank, and the pressing medicine adding assembly is rotatably installed in the inside of the storage tank; the bevel gear linkage assembly is meshedly connected with the bottom of the spline transmission assembly, and the bottom of the driving telescopic frame is fixedly connected with the top of the storage tank.

[0012] Further, the driving telescopic frame comprises two top frames, a hanger rod, a lifting bottom frame and a driving bevel gear; the two top frames are movably installed on the two sides of the top of the lifting bottom frame through the hanger rod; the driving bevel gear is installed on the top of one side of the top frame through a shaft support, and the driving bevel gear extends to the top of the protective cover and is rotatably connected.

[0013] Further, the rack lifting frame comprises a dovetail convex frame, a fixed rack and a movable tooth; the bottom of the dovetail convex frame is fixedly connected with the lifting bottom frame; the fixed rack is fixedly installed on the inner wall of the dovetail convex frame; and the movable tooth is installed on the top of the dovetail convex frame through a shaft piece.

[0014] Further, the gear transmission assembly is rotatably installed between and through the two top frames, and is composed of a driving gear and two driving bevel gears, the two driving bevel gears are fixedly installed on the two sides of the driving gear through shaft rods, and one of the two driving bevel gears is in meshing connection with the driving bevel gear.

[0015] Further, the spline transmission assembly is composed of a first bevel gear, a spline rod, a spline sleeve rod and a second bevel gear, the first bevel gear is in meshing connection with the other driving bevel gear, the top of the spline rod is fixedly installed at the bottom of the first bevel gear, the spline sleeve rod is movably sleeved at the outside of the spline rod, a spline groove is formed in the top wall inside the spline sleeve rod, and a spline hole is formed in the inside of the second bevel gear, the second bevel gear is movably sleeved at the outside of the spline sleeve rod through the spline hole.

[0016] Further, the bevel gear linkage assembly is composed of a top cover, a transmission bevel gear set and a transmission bevel gear disc, the top cover is fixedly installed at the top of the storage tank, the two sides of the top cover are fixedly installed in the inside of the lifting base frame, the transmission bevel gear set is installed through a bearing at one side of the storage tank, the transmission bevel gear disc is rotatably installed in the inside of the top cover, one end of the transmission bevel gear set extending into the inside of the top cover is in meshing connection with the transmission bevel gear disc, and the other end of the transmission bevel gear set extending out of the inside of the top cover is in meshing connection with the second bevel gear.

[0017] Further, the downward pressing and dosing assembly is composed of a lead screw, a lead sleeve and a piston, the top of the lead screw penetrates through the storage tank and is fixedly connected with the bottom of the transmission bevel gear disc, the bottom of the lead screw penetrates through the storage tank and is fixedly connected with the top of the linkage bevel gear disc, the lead sleeve is sleeved at the outside of the lead screw, and the lead sleeve is embeddedly installed in the inside of the piston, and the piston is movably installed in the inside of the storage tank.

[0018] Further, the liquid-stopping telescopic assembly is composed of a third bevel gear, a threaded sleeve, a threaded rod and a telescopic rod, the third bevel gear is fixedly installed at one end of the threaded sleeve, the outside of the threaded sleeve is installed at the bottom of the storage tank through a shaft, one end of the threaded rod is threadedly installed in the inside of the threaded sleeve, one end of the telescopic rod is fixedly connected with the other end of the threaded rod, the telescopic rod movably extends into the inside of the mounting sleeve, and the other end of the telescopic rod is in dynamic contact with the flexible hole seat.

[0019] Further, the inside of the box body is fixedly installed with a hole plate, a test tube is inserted into the inside of the hole plate, a protective cover is threadedly installed at the top of the test tube, a rubber cover is fixedly installed in the inside of the protective cover, and a spring hoop is sleeved at the outside of the rubber cover.

[0020] A detection method of a tumor chemotherapy drug sensitivity detection kit, the detection method comprising the following steps:

[0021] Step one: open the box cover, two equal amount of drug adding mechanism respectively installed in the chemotherapy drugs, lysing solution, twist the protective cover and test tube separation, respectively into six test tubes in the same amount of tumor cells to be detected, and then twist the protective cover sealed test tube, and then insert the six test tubes into the hole plate inside;

[0022] Step two: cover the box cover, hand the extension end of the driving bevel gear to twist, make the driving bevel gear rotate drive meshing connection gear transmission assembly rotation, rotating gear transmission assembly drive rack lifting frame down, the rack lifting frame down through the lifting chassis drives the storage tank down, the spline transmission assembly is affected by gravity to extend downward;

[0023] The down-moving storage tank drives the six groups of injection sampleers in the bottom annular array to move down and penetrate the rubber cover, and extend to the inside of the test tube. At this time, the injection sampleer expands the rubber cover and expands the spring hoop.

[0024] When the spline transmission assembly extends to the set length, it reaches the spline clamping position, realizes the transmission of rotary force, and transmits the rotary force to the bevel gear linkage assembly through the spline transmission assembly. The bevel gear linkage assembly drives the downward adding component to operate cooperatively with the power transmission mechanism, and the running power transmission mechanism opens the injection channel of the six groups of injection sampleers. At the same time, the running downward adding component transports the chemotherapy drugs in the storage tank to the inside of the test tube through the injection sampleer.

[0025] Step three: after completing the equal amount of chemotherapy drug delivery, reverse twist the driving bevel gear, and drive the meshing connection gear transmission assembly to rotate reversely by using the reverse rotating driving bevel gear. The reverse rotating gear transmission assembly drives the meshing connection rack lifting frame to move up, and drives the meshing connection spline transmission assembly to rotate reversely.

[0026] The reverse rotating spline transmission assembly drives the transmission bevel gear set to rotate reversely through the meshing connection, and drives the transmission bevel disc to rotate reversely through the meshing connection. The reverse rotating transmission bevel disc drives the silk sleeve to move up through the screw rod. The silk sleeve sucks the liquid medicine in the injection channel in the flexible hole seat. At the same time, the reverse rotating screw rod drives the linkage bevel disc to rotate reversely, drives the liquid stopping expansion component to extend, and extrudes and deforms the flexible hole seat to close the injection channel.

[0027] At the same time, the rack lifting frame drives the lifting chassis to move up, and the lifting chassis drives the spline transmission assembly to contract, unlock the spline clamping, and remove the rotary force transmission, so that the flexible hole seat remains in a closed state.

[0028] The lifting chassis drives the six groups of injection sampleers in the bottom annular array to move up and away from the rubber cover, and uses the reset contraction force of the spring hoop to shrink and close the channel of the rubber cover, so that the test tube forms a sealed detection vessel.

[0029] Step four: after the chemotherapy drugs and tumor cells are fully reacted, the box cover is opened, and the six test tubes after the reaction are moved to below another set of equal drug adding mechanisms, the inside of the storage tank of the equal drug adding mechanism stores a lysis solution, steps two and three are repeated, the lysis solution in the inside of the storage tank is equally delivered into the corresponding six test tubes, the cell membrane of the lysis solution is lysed, and intracellular ATP is released;

[0030] Step five: the box cover is opened to take out the six test tubes, the lysed cells in the six test tubes are mixed with the luciferase-luciferin mixed solution, under the catalysis of luciferase, ATP and luciferin react to generate a light signal, the light emission intensity is detected by using an enzyme label instrument, the RLU value is converted into the ATP content through a standard curve, the number of living cells or drug sensitivity is calculated according to the ATP content, and the effect of the chemotherapy drugs is evaluated.

[0031] The beneficial effects of the present application are:

[0032] The technical scheme of the present application drives the rack lifting frame to move downward through the rotating gear transmission assembly, the rack lifting frame moving downward drives the lifting chassis in the driving telescopic frame to move downward along the boom, drives the annular array of six groups of sample injectors at the bottom of the storage tank to move downward, so that the injection heads in the six groups of sample injectors extend to the inside of the test tube through the rubber cap; at the same time, the lifting chassis moving downward drives the spline transmission assembly to extend to the set length, reaches the spline clamping position, and realizes the transmission of rotary force; the spline transmission assembly transmits the rotary force of the gear transmission assembly to the bevel gear linkage assembly connected by meshing; the bevel gear linkage assembly in operation drives the downward pressing medicine adding assembly to operate cooperatively with the power transmission mechanism, the power transmission mechanism in operation drives the six groups of liquid stopping telescopic assemblies to perform the contraction action through the linkage bevel gear disc, away from the side of the flexible hole seat in the six groups of sample injectors, so that the injection channel in the flexible hole seat forms a passage, ensuring that the chemotherapy medicine or lysing solution only starts to flow after the injection head is inserted into the test tube; the chemotherapy medicine in the storage tank is transported to the injection head through the injection channel in the flexible hole seat by the downward pressing medicine adding assembly in operation, the chemotherapy medicine or lysing solution is injected into six test tubes through the injection head, and the opening and closing of the six groups of sample injectors are synchronously controlled through the six groups of liquid stopping telescopic assemblies, so as to realize the accurate control of the injection speed and stroke, ensure the equal amount of chemotherapy medicine or lysing solution in each test tube, realize the equal control of the medicine adding or liquid adding amount of the six groups of detection samples, and ensure the consistency of the reaction conditions; the six groups of sample injectors are designed in an annular array, and the equal speed rotation characteristics of the gear transmission assembly are matched, so as to ensure that the advancing speed and stroke of each injection head are completely consistent; the diameters of the injection channels in the flexible hole seats are uniformly designed, so as to ensure the equal amount of medicine injection in each test tube from the physical structure; through the accurate control of the consistency of the medicine adding amount, the device eliminates the deviation of the sample reaction conditions caused by the difference in injection amount, ensures that all samples in the tumor chemotherapy drug sensitivity detection are in the same initial reaction state, and the consistency of the reaction conditions directly improves the reliability of the tumor chemotherapy drug sensitivity detection result, effectively avoids the misjudgment caused by the difference in sample reaction conditions, and provides reliable technical support for the clinical precise evaluation of tumor cell sensitivity. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 It is the overall structure schematic diagram in the present application;

[0034] Figure 2 It is the internal structure schematic diagram in the present application;

[0035] Figure 3 It is the first state structure schematic diagram of the driving mechanism in the present application;

[0036] Figure 4 It is the second state structure schematic diagram of the driving mechanism in the present application;

[0037] Figure 5The internal structure schematic view of the first state of the driving mechanism in the application;

[0038] Figure 6 The internal structure schematic view of the second state of the driving mechanism in the application;

[0039] Figure 7 The driving telescopic frame is connected with the rack lifting frame, the gear transmission assembly and the spline transmission assembly in the application;

[0040] Figure 8 The internal structure schematic view of the driving telescopic frame in the application;

[0041] Figure 9 The rack lifting frame is connected with the gear transmission assembly in the application;

[0042] Figure 10 The structure schematic view of the rack lifting frame in the application;

[0043] Figure 11 The A partial enlarged view in the application Figure 10

[0044] Figure 12 The structure schematic view of the spline transmission assembly in the application;

[0045] Figure 13 The unfolded structure schematic view of the spline transmission assembly in the application;

[0046] Figure 14 The internal structure schematic view of the equal amount of medicine adding mechanism in the application;

[0047] Figure 15 The power transmission mechanism is connected with the sample injector in the application;

[0048] Figure 16 The liquid stopping telescopic assembly is dynamically connected with the flexible hole seat in the application;

[0049] Figure 17 The structure schematic view of the test tube in the application;

[0050] Figure 18 The B partial enlarged view in the application Figure 17

[0051] Explanation of reference signs:

[0052] ​​1, box body; 101, hole plate; 2, box cover; 3, driving mechanism; 301, protective cover; 302, dovetail concave frame; 303, driving telescopic frame; 3031, top frame; 3032, boom; 3033, lifting chassis; 3034, driving bevel gear; 304, rack lifting frame; 3041, dovetail convex frame; 3042, fixed rack; 3043, movable tooth; 305, gear transmission assembly; 3051, driving gear; 3052, driving bevel gear; 306, spline transmission assembly; 3061, first bevel gear; 3062, spline rod; 3063, spline sleeve rod; 3064, second bevel gear; 4, sample injector; 401, mounting sleeve; 402, flexible hole seat; 403, injection head; 5, test tube; 501, protective cover; 502, rubber cover; 503, spring clamp; 6, equal medicine adding mechanism; 601, storage tank; 602, top cover; 603, transmission bevel gear set; 604, transmission bevel disc; 605, lead screw; 606, lead sleeve; 607, piston; 7, power transmission mechanism; 701, linkage bevel disc; 702, liquid stopping telescopic assembly; 7021, third bevel gear; 7022, threaded sleeve; 7023, threaded rod; 7024, telescopic rod. DETAILED DESCRIPTION

[0053] The technical solutions in the embodiments of the present application will be described below in conjunction with the accompanying drawings. Figure 1 to the accompanying drawings Figure 18 The technical solutions in the embodiments of the present application will be described below in conjunction with the accompanying drawings.

[0054] A tumor chemotherapy drug sensitivity detection kit, comprising a box body 1, two groups of driving mechanisms 3 and two groups of equal medicine adding mechanisms 6, further comprising a box cover 2 installed on one side of the box body 1 through a hinge, and six groups of sample injectors 4 arranged in a ring array at the bottom of the equal medicine adding mechanism 6, the sample injector 4 is composed of a mounting sleeve 401, a flexible hole seat 402 and an injection head 403, the flexible hole seat 402 is fixedly installed inside the mounting sleeve 401, and the injection head 403 is threadedly installed at the bottom of the mounting sleeve 401 and connected with the flexible hole seat 402 in penetration;

[0055] A power transmission mechanism 7 is rotatably installed at the bottom center of the equal medicine adding mechanism 6, and the power transmission mechanism 7 is composed of a linkage bevel disc 701 and six groups of liquid stopping telescopic assemblies 702, the six groups of liquid stopping telescopic assemblies 702 are dynamically engaged with the linkage bevel disc 701, and one end of each of the six groups of liquid stopping telescopic assemblies 702 extends into the inside of the six groups of mounting sleeves 401;

[0056] The two groups of driving mechanisms 3 are respectively embedded and installed in the inside of the box cover 2, and the two groups of equal medicine adding mechanisms 6 are fixedly installed at the bottom of the two groups of driving mechanisms 3; the driving mechanism 3 is composed of a protective cover 301, a dovetail concave frame 302, a driving telescopic frame 303, a rack lifting frame 304, a gear transmission assembly 305 and a spline transmission assembly 306; the dovetail concave frame 302 and the driving telescopic frame 303 are fixedly installed at the top wall inside the protective cover 301, the rack lifting frame 304 is movably installed inside the dovetail concave frame 302, the bottom of the rack lifting frame 304 is fixedly connected with the bottom of the driving telescopic frame 303, the gear transmission assembly 305 is rotatably installed inside the driving telescopic frame 303, and the gear transmission assembly 305 is in meshing connection with the rack lifting frame 304; the spline transmission assembly 306 is installed on one side of the driving telescopic frame 303 through a shaft frame, and one end of the gear transmission assembly 305 is in meshing connection with the bottom of the spline transmission assembly 306;

[0057] The equal medicine adding mechanism 6 is composed of a storage tank 601, a bevel gear linkage assembly and a pressing medicine adding assembly; the bevel gear linkage assembly is fixedly installed at the top of the storage tank 601, and the pressing medicine adding assembly is rotatably installed inside the storage tank 601; the bevel gear linkage assembly is in meshing connection with the bottom of the spline transmission assembly 306, and the bottom of the driving telescopic frame 303 is fixedly connected with the top of the storage tank 601;

[0058] The equal medicine adding mechanism 6 is driven by the driving mechanism 3 to move downward to a specified position, and the equal medicine adding mechanism 6 is driven to run to add equal amounts of injection chemotherapy drugs or detection lysate to six test tubes 5, at the same time, the equal medicine adding mechanism 6 transmits the running power to the power transmission mechanism 7, the power transmission mechanism 7 controls the ring array distribution six groups of sample injectors 4 to open the injection channel, and the equal medicine adding mechanism 6 stores the chemotherapy drugs or detection lysate inside to be injected into the six test tubes 5 in equal amounts;

[0059] Specifically, the driving bevel gear 3034 in the driving telescopic frame 303 drives the meshing connected gear transmission assembly 305, the rotating gear transmission assembly 305 drives the rack lifting frame 304 to move downward, the rack lifting frame 304 drives the lifting chassis 3033 in the driving telescopic frame 303 to move downward along the suspender 3032, and the bottom of the storage tank 601 drives the ring array distribution six groups of sample injectors 4 to move downward, so that the injection heads 403 in the six groups of sample injectors 4 extend to the inside of the test tube 5 through the rubber cover 502;

[0060] The lifting base 3033 moving downward drives the spline transmission assembly 306 to extend to a set length, reaches the spline clamping position, and realizes the transmission of rotary force; the spline transmission assembly 306 transmits the rotary force of the gear transmission assembly 305 to the meshed bevel gear linkage assembly; the running bevel gear linkage assembly drives the downward medicine adding assembly to run cooperatively with the power transmission mechanism 7, the linkage bevel gear disc 701 in the running power transmission mechanism 7 drives six sets of liquid stopping telescopic assemblies 702 to retract, away from one side of the flexible hole seat 402 in the six sets of sample injectors 4, so that the injection channel in the flexible hole seat 402 forms a passage, and ensures that the chemotherapy medicine or the lysis liquid starts to flow only after the injection head 403 is inserted into the test tube 5; the downward medicine adding assembly running at the same time transports the chemotherapy medicine in the storage tank 601 to the injection head 403 through the injection channel in the flexible hole seat 402, and injects the chemotherapy medicine into the six test tubes 5 through the injection head 403, so as to ensure that the injection amount of the chemotherapy medicine or the detection lysis liquid in each test tube 5 is consistent, realize the equal control of the medicine adding or liquid adding amount of the six sets of detection samples, and ensure the consistency of the reaction conditions.

[0061] After the equal transportation of the chemotherapy medicine is completed, the main bevel gear 3034 is reversely screwed, the gear transmission assembly 305 connected in mesh drives the rack lifting frame 304 to move upward, and the spline transmission assembly 306 connected in mesh is reversely rotated; the reversely rotating spline transmission assembly 306 transmits the reversely rotating force to the power transmission mechanism 7 through the bevel gear linkage assembly and the downward medicine adding assembly, drives the six sets of liquid stopping telescopic assemblies 702 in the power transmission mechanism 7 to extend, and abuts against the flexible hole seat 402 to close the injection channel, so as to prevent the medicine from leaking.

[0062] As shown in Figures 6 to 8 the driving telescopic frame 303 is composed of two top frames 3031, a suspender 3032, a lifting base 3033 and a main bevel gear 3034, the two top frames 3031 are movably installed on the two sides of the top of the lifting base 3033 through the suspender 3032, the main bevel gear 3034 is installed on the top of one side of the top frame 3031 through a shaft frame, and the main bevel gear 3034 extends to the top of the protective cover 301 and is rotationally connected;

[0063] The extending end of the main bevel gear 3034 is fixedly installed with a knob, the knob is held to rotate the main bevel gear 3034, the main bevel gear 3034 drives the driving bevel gear 3052 connected in mesh to rotate, the driving bevel gear 3052 drives the driving gear 3051 through a shaft rod to rotate, the rotating driving gear 3051 drives the dovetail lug 3041 through the fixed rack 3042 connected in mesh to move downward, the moving downward dovetail lug 3041 drives the lifting base 3033 to move downward along the suspender 3032, the moving downward lifting base 3033 drives the storage tank 601 to move downward, so that the spline transmission assembly 306 extends downward under the influence of gravity.

[0064] As shown in Figures 9 to 11 The rack lifting frame 304 is composed of a dovetail bracket 3041, a fixed rack 3042 and a movable tooth 3043. The bottom of the dovetail bracket 3041 is fixedly connected with the lifting chassis 3033. The fixed rack 3042 is fixedly installed on the inner wall of the dovetail bracket 3041. The movable tooth 3043 is installed on the top of the dovetail bracket 3041 through a shaft piece.

[0065] The movable tooth 3043 is composed of a moving tooth, two telescopic pieces and two compression springs. The two compression springs are respectively sleeved on the outer sides of the two telescopic pieces. The moving tooth is movably sleeved on the top of the outer sides of the two telescopic pieces. The bottom of the moving tooth is in abutting connection with the two compression springs. The dovetail bracket 3041 is movably installed in the dovetail recess 302. The fixed rack 3042 is in meshing connection with the driving gear 3051. The rotating driving gear 3051 drives the dovetail bracket 3041 to move downward in the dovetail recess 302 through the meshing connection of the fixed rack 3042. At the same time, the movable tooth 3043 moves downward synchronously with the fixed rack 3042. When the movable tooth 3043 moves to the meshing position of the driving gear 3051, the rotating driving gear 3051 drives the moving tooth in the movable tooth 3043. The moving tooth moves on the outer side of the telescopic piece to compress the compression spring, so that the driving gear 3051 cannot drive the movable tooth 3043 to move downward, and the driving gear 3051 continues to rotate.

[0066] As shown in Figures 7 to 9 The gear transmission assembly 305 is rotatably installed between and penetrates through the two top frames 3031. The gear transmission assembly 305 is composed of the driving gear 3051 and two driving bevel gears 3052. The two driving bevel gears 3052 are fixedly installed on the two sides of the driving gear 3051 through shaft rods. One of the two driving bevel gears 3052 is in meshing connection with the driving bevel gear 3034.

[0067] The rotating driving bevel gear 3052 is matched with the driving gear 3051 through a shaft rod, so as to ensure that the two driving bevel gears 3052 rotate synchronously. The driving bevel gear 3052 is driven to rotate by the driving bevel gear 3034 in meshing connection. The driving bevel gear 3052 drives the driving gear 3051 to rotate through a shaft rod. The driving gear 3051 drives the other driving bevel gear 3052 to rotate synchronously through a shaft rod, so as to drive the first bevel gear 3061 in meshing connection to rotate.

[0068] As shown in Figures 11 to 12As shown, the spline transmission assembly 306 is composed of a first bevel gear 3061, a spline rod 3062, a spline sleeve rod 3063 and a second bevel gear 3064, the first bevel gear 3061 is in meshing connection with the other driving bevel gear 3052, the top of the spline rod 3062 is fixedly installed at the bottom of the first bevel gear 3061, the spline sleeve rod 3063 is movably sleeved at the outside of the spline rod 3062, and the top wall inside the spline sleeve rod 3063 is provided with a spline groove, and the inside of the second bevel gear 3064 is provided with a spline hole, and the second bevel gear 3064 is movably sleeved at the outside of the spline sleeve rod 3063 through the spline hole;

[0069] Wherein, the spline hole inside the spline sleeve rod 3063 is lowered to the spline end of the bottom end of the spline rod 3062, the driving bevel gear 3052 in rotation drives the spline rod 3062 in rotation through the meshing connection of the first bevel gear 3061, and the spline rod 3062 in rotation drives the second bevel gear 3064 in rotation through the spline connection of the spline sleeve rod 3063.

[0070] As shown in Figure 5 , Figure 14 The bevel gear linkage assembly is composed of a top cover 602, a transmission bevel gear set 603 and a transmission bevel gear disc 604, the top cover 602 is fixedly installed at the top of the storage tank 601, and the two sides of the top cover 602 are fixedly installed inside the lifting underframe 3033, the transmission bevel gear set 603 is installed on one side of the storage tank 601 through a bearing, the transmission bevel gear disc 604 is rotatably installed inside the top cover 602, and one end of the transmission bevel gear set 603 extending to the inside of the top cover 602 is in meshing connection with the transmission bevel gear disc 604, and one end of the transmission bevel gear set 603 extending out of the inside of the top cover 602 is in meshing connection with the second bevel gear 3064;

[0071] Wherein, when the lifting underframe 3033 moves up and down, since the two sides of the top cover 602 are fixedly installed inside the lifting underframe 3033, and the top cover 602 is fixedly installed at the top of the storage tank 601, the storage tank 601 is driven to move up and down; the second bevel gear 3064 in rotation drives the transmission bevel gear disc 604 in rotation through the meshing connection of the transmission bevel gear set 603, and the transmission bevel gear disc 604 in rotation drives the lead screw 605 in rotation.

[0072] As shown in Figure 5 , Figure 14 The downward pressing and dosing assembly is composed of a lead screw 605, a lead sleeve 606 and a piston 607, the top of the lead screw 605 penetrates the storage tank 601 and is fixedly connected with the bottom of the transmission bevel gear disc 604, the bottom of the lead screw 605 penetrates the storage tank 601 and is fixedly connected with the top of the linkage bevel gear disc 701, the lead sleeve 606 is sleeved outside the lead screw 605, and the lead sleeve 606 is embeddedly installed inside the piston 607, and the piston 607 is movably installed inside the storage tank 601;

[0073] Wherein, the rotating lead screw 605 drives the outer side of the lead sleeve 606 to move linearly, and drives the linkage bevel gear 701 to rotate; the linearly moving lead sleeve 606 drives the piston 607 to move downward, so that the chemotherapy drugs inside the storage tank 601 are transported to the inside of the injection head 403 through the injection channel inside the flexible hole seat 402, achieving the purpose of equal injection.

[0074] As shown in Figures 14 to 15 , the liquid-stopping telescopic assembly 702 is composed of a third bevel gear 7021, a threaded sleeve 7022, a threaded rod 7023 and a telescopic rod 7024, the third bevel gear 7021 is fixedly installed at one end of the threaded sleeve 7022, the outer side of the threaded sleeve 7022 is installed at the bottom of the storage tank 601 through a shaft, one end of the threaded rod 7023 is threadedly installed inside the threaded sleeve 7022, one end of the telescopic rod 7024 is fixedly connected with the other end of the threaded rod 7023, and the telescopic rod 7024 is movably extended into the inside of the mounting sleeve 401, the other end of the telescopic rod 7024 is dynamically in contact with the flexible hole seat 402;

[0075] Wherein, the third bevel gear 7021 in driving engagement is rotated, the rotating third bevel gear 7021 drives the threaded rod 7023 to move linearly through the threaded sleeve 7022, the linearly moving threaded rod 7023 drives the telescopic rod 7024 to move telescopically inside the mounting sleeve 401; the extended telescopic rod 7024 extrudes one side of the flexible hole seat 402, so that the flexible hole seat 402 is deformed to close the injection channel; the retracted telescopic rod 7024 moves away from one side of the flexible hole seat 402, opening the injection channel inside the flexible hole seat 402.

[0076] As shown in Figures 17 to 18 , the inside of the box body 1 is fixedly installed with a hole plate 101, the inside of the hole plate 101 is inserted with a test tube 5, the top of the test tube 5 is threadedly installed with a protective cover 501, the inside of the protective cover 501 is fixedly installed with a rubber cover 502, and the outer side of the rubber cover 502 is sleeved with a spring clip 503;

[0077] Wherein, the rubber cover 502 is deformed and expanded when extruded by the injection head 403, generating an expansion force on the spring clip 503, when the rubber cover 502 is separated from the injection head 403, the spring clip 503 is reset to contract, closing the channel of the rubber cover 502, ensuring the sealing effect of the rubber cover 502.

[0078] A detection method of a tumor chemotherapy drug sensitivity detection kit, the detection method comprising the following steps:

[0079] Step one: open the box cover 2, two equal amount of drug mechanism 6 respectively installed in the chemotherapy drugs, lysate, twist protection cover 501 and test tube 5 separate, the tumor cells to be detected into six test tubes 5 respectively, and then twist the protection cover 501 sealed test tube 5, and then six test tubes 5 are inserted into the hole plate 101 inside; Six test tubes 5 ring array distribution in the lower part of the equal amount of drug mechanism 6 with chemotherapy drugs, and six test tubes 5 and the equal amount of drug mechanism 6 bottom six groups of sample injector 4 one to one correspondence;

[0080] Step two: cover the box cover 2, hand the extension end of the driving bevel gear 3034 knob for twisting, make the driving bevel gear 3034 rotation drive meshing connection gear transmission assembly 305 rotation, specifically through the driving bevel gear 3034 drive meshing connection driving bevel gear 3052 rotation, driving bevel gear 3052 through the shaft rod driving gear 3051 rotation, gear 3051 through the shaft rod driving another driving bevel gear 3052 rotation, drive corresponding meshing connection first bevel gear 3061 rotation;

[0081] Rotating driving gear 3051 drive rack and pinion lift 304 down, the rack and pinion lift 304 down through the lifting chassis 3033 drive storage tank 601 down, drive spline transmission assembly 306 to extend downward;

[0082] Wherein, the rotating driving gear 3051 through the meshing connection of fixed rack 3042 drive dovetail lug 3041 down, when the fixed rack 3042 drive movable tooth 3043 down and drive bevel gear 3052 meshing, movable activity tooth 3043 limit the dovetail lug 3041 down;

[0083] Downward movement of the storage tank 601 drive the bottom ring array of six groups of sample injector 4 down, so that the six groups of sample injector 4 injection head 403 through the rubber cover 502, extend to the inside of the test tube 5, at this time the sample injector 4 to the rubber cover 502 expansion spring hoop 503 expansion;

[0084] And spline transmission assembly 306 to the set length, reach the spline card joint position, realize the transmission of rotary force, through the spline transmission assembly 306 rotary force transmission to meshing connection bevel gear linkage assembly; Specifically, through the spline transmission assembly 306 rotary force transmission to meshing connection transmission bevel gear set 603 drive transmission bevel gear 604 rotation;

[0085] The spline transmission assembly 306 drives the rotating power, specifically through the spline hole inside the downward spline sleeve rod 3063 to the spline end of the spline rod 3062 at the bottom to be clamped, the rotating gear transmission assembly 305 drives the spline rod 3062 to rotate through the meshing connection of the first bevel gear 3061, the rotating spline rod 3062 drives the second bevel gear 3064 to rotate through the spline connection of the spline sleeve rod 3063, and the rotating second bevel gear 3064 drives the transmission bevel gear set 603 to rotate through the meshing connection, and the transmission bevel gear set 603 drives the transmission bevel gear disc 604 to rotate;

[0086] The bevel gear linkage assembly drives the downward pressing and dosing assembly to operate cooperatively with the power transmission mechanism 7, the operating power transmission mechanism 7 opens six groups of injection channels of the sample injector 4; at the same time, the operating downward pressing and dosing assembly transports the chemotherapeutic drugs in the storage tank 601 to the inside of the test tube 5 through the sample injector 4; specifically, the transmission bevel gear disc 604 drives the lead screw 605 to rotate, the rotating lead screw 605 drives the outside silk sleeve 606 to move linearly, and drives the linkage bevel gear disc 701 to rotate, the rotating linkage bevel gear disc 701 drives the liquid stopping telescopic assembly 702 to contract, and opens the internal channel of the flexible hole seat 402 in the sample injector 4; at this time, the linearly moving silk sleeve 606 drives the piston 607 to move downward, and the chemotherapeutic drugs in the storage tank 601 are transported to the inside of the injection head 403 through the injection channel in the flexible hole seat 402, and then are transported to the inside of the test tube 5 through the injection head 403;

[0087] Step three: after completing the equal transportation of the chemotherapeutic drugs, the main driven bevel gear 3034 is unscrewed in reverse, the meshing connected gear transmission assembly 305 is driven to rotate in reverse by the reverse rotating main driven bevel gear 3034, the meshing connected gear rack lifting frame 304 is driven to move upward by the reverse rotating gear transmission assembly 305, and the meshing connected spline transmission assembly 306 is driven to rotate in reverse;

[0088] The reverse rotating spline transmission assembly 306 drives the transmission bevel gear set 603 to rotate in reverse through the meshing connection, the transmission bevel gear disc 604 is driven to rotate in reverse by the lead screw 605, the silk sleeve 606 is driven to move upward by the reverse rotating lead screw 605, the silk sleeve 606 sucks the liquid medicine in the injection channel in the flexible hole seat 402; at the same time, the reverse rotating lead screw 605 drives the linkage bevel gear disc 701 to rotate in reverse to drive the liquid stopping telescopic assembly 702 to extend to extrude and deform the flexible hole seat 402 to close the injection channel; the closed injection channel is specifically driven by the rotating linkage bevel gear disc 701 to drive the meshing connected third bevel gear 7021 to rotate, the third bevel gear 7021 drives the threaded rod 7023 to move linearly through the threaded sleeve 7022, the linearly moving threaded rod 7023 drives the telescopic rod 7024 to move to extrude the flexible hole seat 402, so that the flexible hole seat 402 is deformed to close the injection channel;

[0089] Meanwhile, the rack and pinion lifting frame 304 drives the lifting chassis 3033 to move upwards, and the lifting chassis 3033 moving upwards drives the spline transmission assembly 306 to contract, unlocks the spline clamping, removes the rotation force transmission, and makes the flexible hole seat 402 keep a closed state.

[0090] The lifting chassis 3033 moving upwards drives the bottom annular array of six groups of sample injectors 4 to move upwards, and when the injection head 403 in the six groups of sample injectors 4 is separated from the inside of the rubber cover 502, the spring clip 503 is reset to contract the closed channel of the rubber cover 502, so that the test tube 5 forms a sealed detection vessel.

[0091] Step four: after the chemotherapy drugs and tumor cells are fully reacted, the box cover 2 is opened, and the six test tubes 5 after reaction are moved to below another group of equal drug adding mechanisms 6, the inside of the storage tank 601 in the equal drug adding mechanism 6 stores a lysis solution, steps two and three are repeated, the lysis solution in the storage tank 601 is equally delivered to the corresponding six test tubes 5, the cell membrane of the lysis solution is lysed, and the intracellular ATP is released.

[0092] Step five: the box cover 2 is opened to take out the six test tubes 5, the lysed cells in the six test tubes 5 are mixed with the luciferase-luciferin mixed solution, under the catalysis of the luciferase, the ATP reacts with the luciferin to generate a light signal, the luminometer is used to detect the light intensity, the RLU value is converted into the ATP content through a standard curve, the number of living cells or drug sensitivity is calculated according to the ATP content, and the effect of the chemotherapy drugs is evaluated.

[0093] The above-mentioned embodiments can also be changed and modified by those skilled in the art. Therefore, the present application is not limited to the specific embodiments disclosed and described above, and some modifications and changes of the present application should also fall within the protection scope of the claims of the present application. In addition, although some specific terms are used in the specification, these terms are only for convenience of description and do not constitute any limitation on the present application.

Claims

1. A tumor chemotherapy drug sensitivity testing kit, comprising a housing (1), two sets of driving mechanisms (3) and two sets of equal-volume drug dispensing mechanisms (6), characterized in that, It also includes a box cover (2) that is hinged to one side of the box body (1), and six sets of injectors (4) arranged in a ring array at the bottom of the equal volume dosing mechanism (6). The injector (4) consists of a mounting sleeve (401), a flexible orifice seat (402) and an injection head (403). The flexible orifice seat (402) is fixedly installed inside the mounting sleeve (401). The bottom of the mounting sleeve (401) is threaded with an injection head (403), and the injection head (403) is connected to the flexible orifice seat (402). The bottom center of the equal dosage dosing mechanism (6) is rotatably equipped with a power transmission mechanism (7), and the power transmission mechanism (7) consists of a linkage bevel gear disc (701) and six sets of liquid-stopping telescopic components (702). The six sets of liquid-stopping telescopic components (702) are dynamically engaged with the linkage bevel gear disc (701), and one end of each of the six sets of liquid-stopping telescopic components (702) extends into the interior of the six sets of mounting sleeves (401). Two sets of drive mechanisms (3) are respectively embedded inside the box cover (2), and two sets of equal dosage dosing mechanisms (6) are fixedly installed at the bottom of the two sets of drive mechanisms (3); the drive mechanism (3) is composed of a protective cover (301), a dovetail bracket (302), a drive telescopic bracket (303), a rack and pinion lifting bracket (304), a gear transmission assembly (305), and a spline transmission assembly (306). The dovetail bracket (302) and the drive telescopic bracket (303) are both fixedly installed on the top wall inside the protective cover (301), and the rack and pinion lifting bracket (304) is fixedly installed on the top wall inside the protective cover (301). 304) is movably installed inside the dovetail recess (302), and the bottom of the rack lifting frame (304) is fixedly connected to the bottom of the drive telescopic frame (303). The gear transmission assembly (305) is rotatably installed inside the drive telescopic frame (303), and the gear transmission assembly (305) is meshed with the rack lifting frame (304). The spline transmission assembly (306) is installed on one side of the drive telescopic frame (303) through the shaft frame, and one end of the gear transmission assembly (305) is meshed with the bottom of the spline transmission assembly (306). The rack and pinion lifting frame (304) consists of a dovetail bracket (3041), a fixed rack (3042), and a movable tooth (3043). The bottom of the dovetail bracket (3041) is fixedly connected to the lifting base frame (3033). The fixed rack (3042) is fixedly installed on the inner wall of the dovetail bracket (3041). The movable tooth (3043) is installed on the top of the dovetail bracket (3041) through a shaft. The gear transmission assembly (305) is rotatably mounted between two top frames (3031) and passes through the two top frames (3031). The gear transmission assembly (305) consists of a drive gear (3051) and two drive bevel gears (3052). The two drive bevel gears (3052) are fixedly mounted on both sides of the drive gear (3051) by a shaft. One of the drive bevel gears (3052) meshes with the active bevel gear (3034). The movable tooth (3043) consists of a moving tooth, two telescopic members, and two compression springs. The two compression springs are respectively sleeved on the outside of the two telescopic members. The moving tooth is movably sleeved on the top of the outside of the two telescopic members, and the bottom of the moving tooth abuts against the two compression springs. The dovetail bracket (3041) is movably installed inside the dovetail recess (302), and the fixed rack (3042) is meshed with the drive gear (3051). The rotating drive gear (3051) drives the dovetail bracket (3041) to move in the dovetail recess through the meshing fixed rack (3042). The frame (302) moves downward inside, and the movable tooth (3043) moves downward synchronously with the fixed rack (3042). When the movable tooth (3043) moves to engage with the drive gear (3051), the rotating drive gear (3051) pushes the movable tooth in the movable tooth (3043). The pushed movable tooth moves outside the telescopic member to compress the compression spring, so that the drive gear (3051) cannot drive the movable tooth (3043), thus limiting the downward stroke of the dovetail bracket (3041) and not affecting the continued rotation of the drive gear (3051). The equal-volume dosing mechanism (6) consists of a storage tank (601), a bevel gear linkage assembly, and a downward dosing assembly. The bevel gear linkage assembly is fixedly installed on the top of the storage tank (601), and the downward dosing assembly is rotatably installed inside the storage tank (601). The bevel gear linkage assembly is meshed with the bottom of the spline transmission assembly (306), and the bottom of the drive telescopic frame (303) is fixedly connected to the top of the storage tank (601). The bevel gear linkage assembly consists of a top cover (602), a transmission bevel gear set (603), and a transmission bevel gear disc (604). The top cover (602) is fixedly installed on the top of the storage tank (601), and both sides of the top cover (602) are fixedly installed inside the lifting base frame (3033). The transmission bevel gear set (603) is installed through a bearing on one side of the storage tank (601). The transmission bevel gear disc (604) is rotatably installed inside the top cover (602), and one end of the transmission bevel gear set (603) extending into the top cover (602) meshes with the transmission bevel gear disc (604). One end of the transmission bevel gear set (603) extending out of the top cover (602) meshes with the second bevel gear (3064). The pressure dosing assembly consists of a lead screw (605), a sleeve (606), and a piston (607). The top of the lead screw (605) passes through the storage tank (601) and is fixedly connected to the bottom of the transmission bevel gear disc (604). The bottom of the lead screw (605) passes through the storage tank (601) and is fixedly connected to the top of the linkage bevel gear disc (701). The sleeve (606) is sleeved on the outside of the lead screw (605) and is embedded in the inside of the piston (607). The piston (607) is movably installed inside the storage tank (601). The liquid-stop telescopic assembly (702) consists of a third bevel gear (7021), a threaded sleeve (7022), a threaded rod (7023), and a telescopic rod (7024). The third bevel gear (7021) is fixedly installed at one end of the threaded sleeve (7022). The outer side of the threaded sleeve (7022) is installed at the bottom of the storage tank (601) through a shaft. One end of the threaded rod (7023) is threaded inside the threaded sleeve (7022). One end of the telescopic rod (7024) is fixedly connected to the other end of the threaded rod (7023), and the telescopic rod (7024) extends movably into the interior of the mounting sleeve (401). The other end of the telescopic rod (7024) dynamically abuts against the flexible hole seat (402).

2. The tumor chemotherapy drug sensitivity detection kit according to claim 1, characterized in that: The drive telescopic frame (303) consists of two top frames (3031), a boom (3032), a lifting base frame (3033), and a drive bevel gear (3034). The two top frames (3031) are movably installed on both sides of the top of the lifting base frame (3033) through the boom (3032). The drive bevel gear (3034) is installed on the top of one side of the top frame (3031) through a shaft frame, and the drive bevel gear (3034) extends to the top of the protective cover (301) for rotational connection.

3. The tumor chemotherapy drug sensitivity detection kit according to claim 1, characterized in that: The spline transmission assembly (306) consists of a first bevel gear (3061), a spline rod (3062), a spline sleeve rod (3063), and a second bevel gear (3064). The first bevel gear (3061) meshes with another driving bevel gear (3052). The top of the spline rod (3062) is fixedly installed at the bottom of the first bevel gear (3061). The spline sleeve rod (3063) is movably sleeved on the outside of the spline rod (3062), and a spline groove is provided on the top wall inside the spline sleeve rod (3063). A spline hole is provided inside the second bevel gear (3064), and the second bevel gear (3064) is movably sleeved on the outside of the spline sleeve rod (3063) through the spline hole.

4. The tumor chemotherapy drug sensitivity detection kit according to claim 1, characterized in that: The box body (1) is fixedly installed with a perforated plate (101), and a test tube (5) is inserted into the perforated plate (101). A protective cover (501) is threaded onto the top of the test tube (5). A rubber cover (502) is fixedly installed inside the protective cover (501), and a spring clamp (503) is sleeved on the outside of the rubber cover (502).

5. The detection method of a tumor chemotherapy drug sensitivity detection kit according to any one of claims 1 to 4, characterized in that, The detection method includes the following steps: Step 1: Open the box cover (2). The two equal-volume drug delivery mechanisms (6) are filled with chemotherapy drugs and lysis buffer respectively. Twist the protective cap (501) to separate it from the test tube (5). Put the tumor cells to be tested into the six test tubes (5) in equal amounts. Twist the protective cap (501) to seal the test tubes (5). Then insert the six test tubes (5) into the well plate (101). The six test tubes (5) containing the test samples are arranged in a ring array below the equal-volume drug delivery mechanism (6) containing chemotherapy drugs. The six test tubes (5) correspond one-to-one with the six sets of sample injectors (4) at the bottom of the equal-volume drug delivery mechanism (6). Step 2: Cover the box (2), hold the extended end of the active bevel gear (3034) and turn it to make the active bevel gear (3034) rotate and drive the meshing gear transmission assembly (305) to rotate. The rotating gear transmission assembly (305) drives the rack lifting frame (304) to move down. The moving rack lifting frame (304) drives the storage tank (601) to move down through the lifting base frame (3033), so that the spline transmission assembly (306) extends downward under the influence of gravity. The downward-moving storage tank (601) causes the six sets of samplers (4) in the bottom annular array to move down through the rubber cap (502) and extend into the test tube (5). At this time, the samplers (4) expand the rubber cap (502) and the spring clamp (503). After the spline transmission assembly (306) extends to the set length, it reaches the spline engagement position to realize the transmission of rotational force. The rotational force is transmitted to the meshing bevel gear linkage assembly through the spline transmission assembly (306). The bevel gear linkage assembly drives the pressure dosing assembly and the power transmission mechanism (7) to work together. The running power transmission mechanism (7) opens the injection channels of the six sets of injectors (4). At the same time, the running pressure dosing assembly delivers the chemotherapy drugs inside the storage tank (601) to the test tube (5) through the injector (4). Step 3: After completing the delivery of equal amounts of chemotherapy drugs, the active bevel gear (3034) is rotated in the opposite direction. The reverse-rotating active bevel gear (3034) drives the meshing gear transmission assembly (305) to rotate in the opposite direction. The reverse-rotating gear transmission assembly (305) drives the meshing rack lifting frame (304) to move upward and drives the meshing spline transmission assembly (306) to rotate in the opposite direction. The reverse-rotating spline transmission assembly (306) drives the transmission bevel gear disk (604) to rotate in the opposite direction through the meshing transmission bevel gear set (603). The reverse-rotating transmission bevel gear disk (604) drives the threaded sleeve (606) to move upward through the lead screw (605). The upward-moving threaded sleeve (606) draws the liquid in the injection channel inside the flexible orifice seat (402). At the same time, the reverse-rotating lead screw (605) drives the linkage bevel gear disk (701) to rotate in the opposite direction, driving the liquid-stopping telescopic assembly (702) to extend and squeeze and deform the flexible orifice seat (402) to close the injection channel. At the same time, the upward-moving rack lifting frame (304) drives the lifting base frame (3033) to move upward, and the upward-moving lifting base frame (3033) drives the spline transmission assembly (306) to retract, unlocking the spline engagement, releasing the rotational force transmission, so that the flexible hole seat (402) remains in a closed state; The upward-moving lifting base (3033) drives the six sets of samplers (4) in the bottom ring array to move upward away from the rubber cap (502). The spring clamp (503) resets the contraction force to shrink and close the channel of the rubber cap (502), so that the test tube (5) forms a sealed detector dish. Step 4: After the chemotherapy drugs have fully reacted with the tumor cells, open the box lid (2) and move the six test tubes (5) after the reaction to the bottom of another set of equal volume dosing mechanism (6). The storage tank (601) in the equal volume dosing mechanism (6) contains lysis solution. Repeat steps 2 and 3 to deliver the lysis solution inside the storage tank (601) to the corresponding six test tubes (5) in equal volume. The cell membrane of the lysis solution is lysed, releasing intracellular ATP. Step 5: Open the box (2) and take out six test tubes (5). Mix the lysed cells in the six test tubes (5) with the luciferase-luciferin mixture. Under the catalysis of luciferase, ATP reacts with luciferin to generate a light signal. Use an enzyme-linked immunosorbent assay (ELISA) reader to detect the light intensity. Convert the RLU value into ATP content through a standard curve. Calculate the number of live cells or drug sensitivity based on the ATP content to evaluate the effect of chemotherapy drugs.

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