Planting method of high-quality medical industrial cannabis sativa

Abstract

The invention provides a planting method of high-quality medical industrial hemp, and belongs to the technical field of hemp planting. Planting is conducted indoors or in a greenhouse, a planting system is filled with CO2 gas, a plant growth lamp is arranged, and a composite nano polysaccharide bioestrogen fertilizer is applied. The composite nano polysaccharide disclosed by the invention is a novel biological material formed by fermenting acetobacter aceti, has relatively high biocompatibility, applicability and good biodegradability, and can be used for enhancing the disease and pest resistance of crops, improving the absorption and utilization rate of the crops to fertilizers and water, promoting the growth of plants and improving the quality and yield of the crops.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cannabis planting, in particular to a planting method of high-quality medicinal industrial hemp. BACKGROUND

[0002] Cannabis sativa L., a one-year-old herbaceous plant, is also known as Shan Simiao, Linma, Huma, Wild Hemp, and Huoma. In China, hemp has a history of several thousand years of application, and is widely used in textiles, building materials, medicine, food, and other fields. The main use of medicinal industrial hemp is to extract effective components for medical purposes, and the content of effective components determines the value.

[0003] Plant growth cannot be separated from photosynthesis, and stable artificial light source irradiation can eliminate the influence of climate and planting site on the quality of harvested effective components, thereby ensuring stability and consistency and meeting the needs of current industrial development. Therefore, with the development of plant industrialization and industrialization, how to obtain high-quality plant effective components and large-scale planting technology has become an urgent need.

[0004] Biological hormones are derived from naturally occurring substances in nature, rich in active substances, and are not artificially synthesized by chemical synthesis and can directly act on plants. For example, common seaweed extract, humic acid (including mineral potassium fulvate), microbial bacteria, chitin (chitosan), and amino acids. Biological hormones are different from plant protection products (hormones) and general fertilizers. Such substances will not cause any harmful effects on humans, animals, and the environment. Biological hormones do not directly act on plants, but through indirect conversion, stimulate and activate plants to absorb, defend, improve the physiological and biochemical state of plants, improve the utilization rate of pesticides and fertilizers, improve the resistance of crops in adverse environments, and also improve the final yield of crops and the quality of agricultural products.

[0005] In summary, light and fertilizer utilization are the most important ways for medicinal industrial hemp to obtain nutrients, which directly affect yield and effective component content. SUMMARY

[0006] The application aims at a high-quality medicinal industrial hemp planting method, which comprises planting in a room or a greenhouse, filling 400-1500 mg / kg CO2 gas in a planting system, setting a plant growth lamp, and applying a composite nanopolysaccharide bio-estrogen fertilizer.

[0007] The application discloses a high-quality medicinal industrial hemp planting method, which is characterized by planting in a room or a greenhouse, filling CO2 gas in a planting system, setting a plant growth lamp, and applying a composite nanopolysaccharide bio-estrogen fertilizer, so that the growth of medicinal industrial hemp and the accumulation of effective components can be effectively promoted.

[0008] As a further improvement of the application, the filling concentration of the CO2 gas is 400-1500 mg / kg.

[0009] As a further improvement of the application, the content of nanocellulose in the composite nanopolysaccharide bio-estrogen fertilizer is greater than 99%, the average particle size of the nanocellulose is 20-30 nm, and the polymerization degree is 2000-8000.

[0010] As a further improvement of the application, the composite nanopolysaccharide bio-estrogen fertilizer is applied by the ways of soil mixing, root irrigation or foliar spraying.

[0011] As a further improvement of the application, the composite nanopolysaccharide bio-estrogen fertilizer is applied after being prepared into a water solution with a concentration of 0.01-10 v / v%.

[0012] As a further improvement of the application, the preparation method of the composite nanopolysaccharide bio-estrogen fertilizer is as follows:

[0013] Chinese medicine residues are added into water, sterilized, inoculated with acetic acid bacillus seed liquid, added with composite enzymes, fermented and enzymatically hydrolyzed, filtered, the filtrate is added with ethanol for precipitation, the solid is washed and dried to obtain the composite nanopolysaccharide bio-estrogen fertilizer.

[0014] As a further improvement of the application, the inoculation amount of the acetic acid bacillus seed liquid is 2-3 v / v%, the bacterial content of the seed liquid is 10 8 -10 9 cfu / mL.

[0015] As a further improvement of the present invention, the mass ratio of the Chinese herbal medicine residue to the compound enzyme is 20-30:1-2.

[0016] As a further improvement of the present invention, the composite enzyme is cellulase and pectinase in a mass ratio of 10:3-5.

[0017] As a further improvement of the present invention, the fermentation and enzymatic hydrolysis culture conditions are 25-30℃, 100-200r / min, and fermentation and enzymatic hydrolysis culture for 24-36h.

[0018] The present invention has the following beneficial effects:

[0019] The composite nanopolysaccharide of this invention is a novel biomaterial produced by fermentation using Acetic Acid Bacillus. It possesses characteristics such as high crystallinity (95%), high purity (containing over 99% nanocellulose), high Young's modulus, high water holding capacity, and a three-dimensional nanostructure. It also exhibits high biocompatibility, adaptability, and good biodegradability. However, there is currently no research on the application of composite nanopolysaccharides in improving plant quality and promoting yield.

[0020] The composite nanopolysaccharide (nBT) of this invention is derived from the fermentation of Acetic Acid Bacillus. The composite nanopolysaccharide contains more than 99% nanocellulose, requiring no purification. The nanocellulose has a high uniformity of 20-30 nm, a 3D structure, and a degree of polymerization (DP value) of 2000-8000. The composite nanopolysaccharide of this invention has high biocompatibility, applicability, and good biodegradability. It can enhance the crop's resistance to diseases and pests, improve the crop's absorption and utilization of fertilizers and water, promote plant growth, and improve crop quality and yield. Detailed Implementation

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] Preparation Example 1

[0023] This preparation example provides a method for preparing a composite nano-polysaccharide bio-estrogen fertilizer, including the following steps:

[0024] Add 20g of Chinese herbal medicine residue to 200mL of water, sterilize, and inoculate with a bacterial count of 10. 8 -10 9The seed culture of Acetobacter acetic acid with cfu / mL was inoculated at a rate of 2v / v%, and 1g of compound enzyme was added. The culture was fermented at 25℃ and 100r / min for 24h. After filtration, ethanol was added to the filtrate to precipitate the solid, which was then washed and dried to obtain the composite nanopolysaccharide.

[0025] The composite enzyme is cellulase and pectinase in a mass ratio of 10:3.

[0026] Preparation Example 2

[0027] This preparation example provides a method for preparing a composite nano-polysaccharide bio-estrogen fertilizer, including the following steps:

[0028] Add 30g of Chinese herbal medicine residue to 200mL of water, sterilize, and inoculate with a bacterial count of 10. 8 -10 9 The seed culture of Acetobacter acetic acid with cfu / mL was inoculated at a rate of 3v / v%, and 2g of compound enzyme was added. The culture was fermented at 30℃ and 100-200r / min for 36h. After filtration, the filtrate was precipitated with ethanol, washed with solid, and dried to obtain composite nanopolysaccharides.

[0029] The composite enzyme is cellulase and pectinase in a mass ratio of 10:5.

[0030] Preparation Example 3

[0031] This preparation example provides a method for preparing a composite nano-polysaccharide bio-estrogen fertilizer, including the following steps:

[0032] Add 25g of Chinese herbal medicine residue to 200mL of water, sterilize, and inoculate with a bacterial count of 10. 8 -10 9 Acetobacter acetic acid seed culture with cfu / mL was inoculated at a rate of 2.5 v / v%, and 1.5 g of compound enzyme was added. The culture was fermented at 27℃ and 100-200 r / min for 30 h. The mixture was filtered, and ethanol was added to the filtrate to precipitate the solid. The solid was washed and dried to obtain the composite nanopolysaccharide.

[0033] The composite enzyme is cellulase and pectinase in a mass ratio of 10:4.

[0034] Example 1

[0035] This embodiment provides a method for cultivating high-quality medicinal industrial hemp. The cultivation is carried out indoors, with the cultivation system filled with CO2 gas at a concentration of 400 mg / kg. Plant growth lights are installed, and the compound nano-polysaccharide bio-estrogen fertilizer prepared in Example 1 is applied by foliar spraying. The compound nano-polysaccharide bio-estrogen fertilizer is prepared as a 2 v / v% aqueous solution before application.

[0036] Example 2

[0037] This embodiment provides a method for cultivating high-quality medicinal industrial hemp. The cultivation is carried out indoors or in a greenhouse. The cultivation system is filled with CO2 gas at a concentration of 1500 mg / kg. Plant growth lights are set up, and the composite nano-polysaccharide bio-estrogen fertilizer prepared in Preparation Example 2 is applied by mixing with soil. The composite nano-polysaccharide bio-estrogen fertilizer is prepared as a 10 v / v% aqueous solution before application.

[0038] Example 3

[0039] This embodiment provides a method for cultivating high-quality medicinal industrial hemp. The cultivation is carried out indoors or in a greenhouse. The cultivation system is filled with CO2 gas at a concentration of 1000 mg / kg. Plant growth lights are installed, and the compound nano-polysaccharide bio-estrogen fertilizer prepared in Preparation Example 3 is applied by root irrigation. The compound nano-polysaccharide bio-estrogen fertilizer is prepared as a 5 v / v% aqueous solution before application.

[0040] Test Example 1

[0041] This experiment used DMG245, a medicinal industrial hemp, as the research object. Healthy branches were taken from the same mother plant of DMG245, rooted, and cloned seedlings approximately 12 cm in height with well-developed root systems were selected and transplanted into 5-gallon planting containers. Four treatments were designed: Treatment 1 (blank control): Agricultural compound fertilizer was applied as base fertilizer according to greenhouse crop fertilization methods; Treatment 2: Agricultural compound fertilizer + 0.5% of the compound nano-polysaccharide bio-estrogen fertilizer solution prepared in Example 1 was used as base fertilizer, and the cultivation method was the same as in Example 1; Treatment 3: Agricultural compound fertilizer + 0.5% of the compound nano-polysaccharide bio-estrogen fertilizer solution prepared in Example 2 was used as base fertilizer, and the cultivation method was the same as in Example 2; Treatment 4: Agricultural compound fertilizer + 0.5% of the compound nano-polysaccharide bio-estrogen fertilizer solution prepared in Example 3 was used as base fertilizer, and the cultivation method was the same as in Example 3. All other management measures were carried out according to the conventional greenhouse management of medicinal industrial hemp. During the critical growth period, measurements of growth indicators, cannabidiol (CBD) content, and yield will be conducted.

[0042] Table 1. Effects on industrial hemp yield and other related traits.

[0043]

[0044] Test Example 2

[0045] This experiment used medicinal industrial hemp hmzy0324 as the research object. Healthy branches were taken from the same mother plant of hmzy0324, rooted, and cloned seedlings approximately 10 cm in height with well-developed root systems were selected and transplanted into 5-gallon planting containers. Four treatments were designed: Treatment 1 (blank control): Agricultural compound fertilizer was applied as base fertilizer according to greenhouse crop fertilization methods; Treatment 2: Agricultural compound fertilizer + 0.1% of the compound nano-polysaccharide bio-estrogen fertilizer solution prepared in Example 1 as base fertilizer, with the same cultivation method as Example 1; Treatment 3: Agricultural compound fertilizer + 0.1% of the compound nano-polysaccharide bio-estrogen fertilizer solution prepared in Example 2 as base fertilizer, with the same cultivation method as Example 2; Treatment 4: Agricultural compound fertilizer + 0.1% of the compound nano-polysaccharide bio-estrogen fertilizer solution prepared in Example 3 as base fertilizer, with the same cultivation method as Example 3. All other management measures were carried out according to the conventional greenhouse management of medicinal industrial hemp. During the critical growth period, harvest growth indicators, cannabinoid (CBG) content, and yield will be measured.

[0046] Table 2. Effects on industrial hemp yield and other related traits.

[0047]

[0048] The results are shown in Tables 1 and 2. Appropriate plant cultivation methods, including specific plant growth lamp wavelengths, high CO2 concentrations, and environmentally friendly compound nano-polysaccharide fertilizers, can effectively promote the growth of hemp seedlings, significantly promote the yield of flowers and leaves, significantly promote the increase of the dry weight of flowers and leaves, and effectively promote the accumulation of important active ingredients in plants.

[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for cultivating high-quality medicinal industrial hemp, characterized in that, It is grown indoors or in a greenhouse, with the planting system filled with CO2 gas, plant growth lights installed, and compound nano-polysaccharide bio-estrogen fertilizer applied.

2. The method for cultivating high-quality medicinal industrial hemp according to claim 1, characterized in that, The CO2 gas concentration is 400-1500 mg / kg.

3. The method for cultivating high-quality medicinal industrial hemp according to claim 1, characterized in that, The composite nanopolysaccharide bio-estrogen fertilizer contains more than 99% nanocellulose, and the average particle size of the nanocellulose is 20-30 nm, with a degree of polymerization of 2000-8000.

4. The method for cultivating high-quality medicinal industrial hemp according to claim 1, characterized in that, The composite nanopolysaccharide bio-estrogen fertilizer is applied by mixing with soil, drenching roots, or foliar spraying.

5. The method for cultivating high-quality medicinal industrial hemp according to claim 1, characterized in that, The composite nanopolysaccharide bio-estrogen fertilizer is prepared into an aqueous solution with a concentration of 0.01-10 v / v% before application.

6. The method for cultivating high-quality medicinal industrial hemp according to claim 1, characterized in that, The preparation method of the composite nano-polysaccharide bio-estrogen fertilizer is as follows: Chinese herbal medicine residue is added to water, sterilized, inoculated with Acetobacter acetic acid seed liquid, compound enzyme is added, fermented and enzymatically cultured, filtered, precipitated with ethanol, washed with solid, and dried to obtain compound nano-polysaccharide bio-estrogen fertilizer.

7. The method for cultivating high-quality medicinal industrial hemp according to claim 6, characterized in that, The inoculation amount of the Acetobacter acetic acid seed solution is 2-3 v / v, and the bacterial count of the seed solution is 10. 8 -10 9 cfu / mL.

8. The method for cultivating high-quality medicinal industrial hemp according to claim 6, characterized in that, The mass ratio of the Chinese herbal medicine residue to the compound enzyme is 20-30:1-2.

9. The method for cultivating high-quality medicinal industrial hemp according to claim 6, characterized in that, The compound enzyme is cellulase and pectinase in a mass ratio of 10:3-5.

10. The method for cultivating high-quality medicinal industrial hemp according to claim 6, characterized in that, The fermentation and enzymatic hydrolysis conditions are 25-30℃, 100-200r / min, and fermentation and enzymatic hydrolysis for 24-36h.

Images