Multi-effect skin care essence containing compound essential oil and preparation method thereof

Abstract

The invention relates to multiple-effect skin care essence containing compound essential oil and a preparation method thereof. The multiple-effect skin care essence is characterized by comprising the following raw materials in percentage by mass: a component A: 71.5% of water, 0.03% of disodium EDTA (Ethylene Diamine Tetraacetic Acid) and the like; the component B comprises 0.375% of acrylic acid (ester) copolymer sodium, 0.125% of lecithin and the like; the component C comprises 0.03 to 0.06 percent of 1, 2-hexanediol, 0.2 percent of glycerol, 0.5 to 1.0 percent of compound essential oil and the like; the compound essential oil is prepared from the following raw materials in percentage by mass: 10 to 20 percent of lemon peel oil, 35 to 45 percent of bergamot fruit oil, 1 to 10 percent of osmanthus flower extract, 3 to 5 percent of lavender oil, 1 to 10 percent of pelargonium graveolens leaf oil, 15 to 25 percent of lime peel oil and 5 to 15 percent of juniper oil. The lotion has special and pleasant flower and fruit fragrance, has the beneficial effect of simultaneously realizing multiple skin care effects of moisturizing, relieving, tightening, resisting wrinkles and the like, and also has the advantages of mildness, no irritation and suitability for various skin types.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically to a multi-effect skin care essence containing compound essential oils and its preparation method. Background Technology

[0002] Serums are considered high-performance skincare products, containing highly active ingredients extracted from natural plants, animals, or minerals. Thanks to their small molecular structure, serums can quickly penetrate the skin's surface, reaching the basal layer to precisely deliver essential nutrients and moisture, effectively repairing damaged cells.

[0003] As consumers' demands for skincare product efficacy diversify, they not only require basic moisturizing functions but also expect benefits such as soothing, anti-wrinkle, and repair. However, most skincare serums on the market often have only one function. Even those claiming multiple effects often suffer from poor actual results due to an unbalanced combination of active ingredients, and some contain irritating ingredients that can easily damage the skin. Therefore, developing a safe, effective, and comprehensive serum that improves skin condition is of significant market importance. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention aims to provide a multi-effect skincare essence containing compound essential oils and its preparation method. This essence, through a unique formula of compound essential oils combined with modern skincare technology, possesses a distinctive and pleasant floral and fruity fragrance, and simultaneously achieves multiple skincare benefits such as moisturizing, soothing, firming, and anti-wrinkle effects. Furthermore, it is gentle and non-irritating, suitable for all skin types.

[0005] The technical solution of this invention is: a multi-effect skin care essence containing compound essential oils, characterized in that it comprises the following raw materials by weight percentage: Component A: Water 71.5%, Disodium EDTA 0.03%, Butylene Glycol 2%, 1,3-Propane Glycol 5%, 1,2-Pentanediol 1.5%, Glycerin 5.0%, Acrylic Acids / C10-30 Alkyl Acrylate Crosspolymer 0.2%, Allantoin 0.2%, Sodium Hyaluronate 0.05%, Polyacrylate Crosspolymer-6 0.1%, Betaine 1.0%, Maltodextrin Glucoside 0.5%; Component B: Sodium acrylate copolymer 0.375%, lecithin 0.125%, polydimethylsiloxane 2.0%, squalane 2.0%, cetyl ethylhexanoate 4.0%, jojoba oil 2.0%, tocopherol (vitamin E) 0.05%, 1,2-hexanediol 0.03%, p-hydroxyacetophenone 0.01%, methyl propylene glycol 0.008%, ethylhexylglycerin 0.003%; Component C: 1,2-Hexanediol 0.03~0.06%, Glycerin 0.2%, Citric Acid 0.04~0.08%, Sodium Citrate 0.001~0.002%, Tetrahydromethylpyrimidine Carboxylic Acid 0.3%, Youthful Skin Peptide 0.3%, Dipeptide Diaminobutyrylbenzylamide Diacetate 0.001~0.002%, Palmitoyl Tripeptide-5 0.002~0.004%, Sugar Isomers 0.07~0.14%, Willowleaf Fritillaria Extract 0.002~0.004%, Edelweiss Flower / Leaf Extract 0.002~0.004%, Panthenol 0.7~1.0%, Compound Essential Oil 0.5~1.0%, Water 0.071%, Aminomethylpropanol 0.1%; The Youthful Skin Peptide is composed of the following components in weight percentage: Egyptian Blue Water Lily (NYMPHAEA) The compound essential oil consists of 90-95% CAERULEA floral water, 1,2-pentanediol (1-5%), 1,2-hexanediol (1-3%), mannitol (1-3%), collagen (0.01-0.2%), water (0.001-0.01%), sodium chloride (0.00001-0.0001%), sodium dihydrogen phosphate (0.00001-0.0001%), and sodium hyaluronate (0.00001-0.0001%). The compound essential oil is composed of the following ingredients in the indicated weight percentages: lemon peel oil (10-20%), bergamot fruit oil (35-45%), osmanthus flower extract (1-10%), lavender oil (3-5%), geranium leaf oil (1-10%), lime peel oil (15-25%), and North American juniper oil (5-15%).

[0006] As a further preferred embodiment of the present invention, the compound essential oil is composed of the following raw materials in weight percentage: 15% lemon peel oil, 40% bergamot fruit oil, 5% osmanthus flower extract, 5% lavender oil, 5% geranium leaf oil, 20% lime peel oil, and 10% North American juniper oil.

[0007] The compound essential oil described in this invention has a special, pleasant floral and fruity aroma.

[0008] This invention also provides a method for preparing a multi-effect skincare essence containing compound essential oils, characterized by comprising the following steps: Step 1) Accurately weigh the raw materials of components A, B, and C according to the formula and place them in sterilized and dry containers for later use; Step 2) Prepare suitable production equipment, such as an emulsifying pot with stirring and heating functions, a thermometer, and a pH meter; Step 3) Phase A: Pour deionized water into the emulsifying pot and stir slowly. Add butanediol, 1,3-propanediol, 1,2-pentanediol, glycerol, acrylates / C10-30 alkanol acrylate crosspolymer, allantoin, sodium hyaluronate, polyacrylate crosspolymer-6, betaine and maltodextrin glucoside, and EDTA-2NA in the order of the formula. Start the heating function and stir continuously until the solution is uniform and clear, so that the components are deeply integrated. Raise the temperature of the emulsifying pot to 85°C and maintain a constant temperature to prepare for subsequent mixing with the oil phase. Step 4) Phase B: In another strictly sterilized sterile container, weigh out the oil phase raw materials such as sodium acrylate copolymer, lecithin, and polydimethylsiloxane according to the precise ratio, stir for 10-15 minutes to fully disperse the components and form a homogeneous oil phase mixture without lumps. Then heat the oil phase mixture to the same temperature as the water phase, i.e., 85°C, to ensure that the temperature is consistent when the two phases are mixed, and to avoid the effect of emulsification due to temperature difference. Step 5) Emulsification process: After the oil phase mixture and the aqueous base liquid have reached the predetermined temperature, slowly pour the oil phase into the aqueous phase, and at the same time turn on the high-speed homogenization function of the emulsification pot to make the oil phase evenly dispersed in the aqueous phase in the form of tiny droplets. Continue the homogenization operation until a fine and stable emulsion system is formed to ensure that the emulsion does not separate or precipitate during storage and use. Step 6) Cooling and adding active ingredients, fragrances, and pH adjusters in phase C: After emulsification, turn off the heating device and allow the emulsion to cool while continuously stirring; when the temperature drops to 40℃-45℃, add 1,2-hexanediol, glycerol, citric acid, sodium citrate, tetrahydromethylpyrimidine carboxylic acid, dermal peptide, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5, saccharide isomers, *Salvia frescoeflora* extract, *Edelweiss* flower / leaf extract, panthenol, and other mixtures in sequence, and finally add the compound essential oil, and continue stirring for 10 min-15 min; Step 7) pH Adjustment and Quality Testing: Use a calibrated pH meter to accurately test the multi-effect skin care essence to ensure that its pH value is stably maintained within the suitable range of 4.0-8.0; if the pH value deviates from the standard range, add an appropriate amount of pH adjuster for fine adjustment according to the professional formula; after completing the pH adjustment, conduct a comprehensive quality test on the finished product, covering multiple dimensions such as appearance, odor, viscosity, and microbial indicators; Step 8) Filling and Packaging: The qualified essence is aseptically filled into pre-sterilized packaging bottles. After filling, the bottles are immediately sealed, labeled, and boxed to ensure the product maintains stable quality during transportation and storage.

[0009] This invention aims to provide a novel, multi-effect serum. Through scientific formulation, it achieves synergistic skincare benefits across multiple dimensions and boasts a floral and fruity fragrance. Polyols, sodium hyaluronate, and sugar isomers work together to construct a highly effective moisturizing system; peptides such as "Youthful Peptides" promote collagen production, achieving anti-wrinkle and firming effects; allantoin, plant extracts, and panthenol work synergistically to soothe and repair the skin. Tocopherol and compound essential oils possess both antioxidant and mood-soothing functions, with the compound essential oils effectively scavenging free radicals even at low concentrations. Furthermore, this invention features a unique fragrance and pleasant texture, enhancing the user experience. Mild preservatives ensure safety, making it suitable for all skin types and providing users with a comprehensive skincare experience.

[0010] The beneficial effects of this invention are: (1) The present invention uses a scientifically proportioned blend of natural plant essential oils to produce a compound essential oil that is fresh and natural with a rich and layered fragrance. Volunteer testing shows that the compound essential oil with a sweet floral and fruity aroma prepared by the present invention gives the essence a unique and pleasant fragrance. During use, it can effectively soothe emotions, calm nerves, reduce stress, and significantly improve the user experience.

[0011] (2) The formula contains a variety of polyol moisturizers, such as butylene glycol, 1,3-propanediol, 1,5-pentanediol, glycerin, and methylpropanediol, which can absorb and lock in moisture in the skin. Sodium hyaluronate, as a highly effective moisturizing ingredient, forms a moisturizing film on the skin surface to prevent moisture loss. The saccharide isomers continuously provide moisture to the skin by binding to the stratum corneum. The synergistic effect of these moisturizing ingredients significantly enhances the skin's moisturizing ability, keeping the skin hydrated.

[0012] (3) Allantoin has anti-inflammatory and cell-repair-promoting effects, which can effectively relieve skin discomfort and accelerate wound healing; Fresnel extract and Edelweiss flower / leaf extract have soothing and anti-inflammatory effects on skin redness and reduce the damage of external stimuli to the skin; Panthenol can penetrate deep into the skin and be converted into vitamin B5, which enhances the skin barrier function and plays a soothing and repairing role. These ingredients work together to effectively soothe the skin and reduce skin sensitivity.

[0013] (4) Tocopherol (vitamin E) is a powerful antioxidant that can capture and neutralize free radicals in the body and protect skin cells from oxidative damage. Even at low concentrations, the sweet floral and fruity essential oils can effectively remove ABTS and DPPH free radicals, further enhancing the antioxidant capacity of the serum, delaying skin aging, and keeping the skin healthy and youthful.

[0014] (5) Peptide ingredients such as youthful peptide, dipeptide diaminobutyryl benzylamide diacetate, and palmitoyl tripeptide-5 can promote collagen production, enhance skin elasticity and firmness, reduce wrinkles, improve skin laxity, make skin firmer and more elastic, and achieve significant anti-wrinkle effects.

[0015] (6) The formula uses mild preservatives such as 1,2-hexanediol, p-hydroxyacetophenone, and ethylhexylglycerin, which reduces skin irritation and avoids the safety hazards that traditional preservatives may bring. This ensures that the serum maintains its efficacy while being highly safe and gentle, making it suitable for all skin types.

[0016] In summary, this invention has a unique floral and fruity fragrance, and can simultaneously achieve all-round care with multiple functions such as moisturizing, soothing, firming, and anti-wrinkle. It also has the advantages of being gentle and non-irritating, making it suitable for all skin types. Attached Figure Description

[0017] Figure 1 The results are from the chicken embryo allantoic membrane (CAM) stimulation test in Examples 1-3.

[0018] Figure 2 This is a comparison chart of the D28 test value and D0 value of the stratum corneum moisture content in Example 4.

[0019] Figure 3 This is a comparison chart of the skin elasticity characterization R value D28 test value and D0 in Example 4.

[0020] Figure 4 This is a comparison chart of the skin firmness characterization F4 value D28 test value and D0 value in Example 4.

[0021] Figure 5 This is a comparison chart of the average skin hemoglobin D28 test value and D0 value in Example 4.

[0022] Figure 6 This is a comparison chart of the D28 test value and D0 value of the crow's feet wrinkle depth in Example 4.

[0023] Figure 7 This is a comparison chart of the D28 test value and D0 value of the crow's feet wrinkle width in Example 4.

[0024] Figure 8 Typical images of skin hemoglobin from some subjects in Example 4.

[0025] Figure 9 The images shown are typical images of crow's feet at the corners of the eyes of some subjects in Example 4. Detailed Implementation

[0026] The present invention will be further described below with reference to the accompanying drawings and specific embodiments: Example 1: Compound essential oil: Weigh the following components according to the following mass percentages and mix them evenly: 15% lemon peel oil, 40% bergamot fruit oil, 5% osmanthus flower extract, 5% lavender oil, 5% geranium leaf oil, 20% lime peel oil, and 10% North American juniper oil to obtain a sweet floral and fruity compound essential oil 1.

[0027] Example 2: Compound essential oil: Weigh the following components according to the following mass percentages and mix them evenly: 10% lemon peel oil, 45% bergamot fruit oil, 1% osmanthus flower extract, 3% lavender oil, 1% geranium leaf oil, 25% lime peel oil, and 15% North American juniper oil to obtain a sweet floral and fruity compound essential oil 2.

[0028] Example 3 Compound Essential Oil: Weigh the following components according to the following mass percentages and mix them evenly: 20% lemon peel oil, 35% bergamot fruit oil, 10% osmanthus flower extract, 5% lavender oil, 10% geranium leaf oil, 15% lime peel oil, and 5% North American juniper oil to obtain a sweet floral and fruity compound essential oil 3.

[0029] Test 1: Preference Evaluation of Compound Essential Oils Thirty healthy participants aged 25-45 years were selected and fitted with a smart emotion-sensing bracelet. Participants rested in a quiet environment for 10 minutes, then applied a small amount of essential oil to a scent paper. Their facial expressions, feelings, and the bracelet's emotion-sensing monitoring were all recorded. The results are shown in Table 1.

[0030] Table 1. Statistics on the number of people who prefer different essential oil scents.

[0031] The above experimental results demonstrate the population's preference for different combinations of essential oil scents. From the perspective of pleasure, the dissatisfaction rate (number of dissatisfied individuals) for Example 1 was lower than that for Examples 2 and 3. This indicates that the scent of Example 1 is more popular, and its fragrance profile is a natural and fresh floral-fruity scent. The top note is a gentle yet spirited sweetness dominated by bergamot, with the aroma of ripe flowers and fruits; the middle note is the highly anticipated geranium paired with the mellow sweetness of osmanthus, a rich floral fragrance with a restrained elegance; the base note is a mellow amber scent with characteristics of cucumber and sandalwood, reminiscent of full, vibrant flowers blooming on a lush tree.

[0032] Test 2: Mildness Test of Compound Essential Oils Referring to the reference standard SN / T 2329-2009 "Cultural Allantoic Membrane Test for Eye Irritation / Corrosivity of Cosmetics", SPF eggs were incubated in a constant temperature incubator at (37.5±5)℃ and a relative humidity of 55%~70% until 9 days of age. The negative control was olive oil; the positive control was 1.0wt% sodium dodecyl sulfate (SDS) solution.

[0033] Add 300 μL of the test sample solution to the CAM surface, ensuring that at least 50% of the CAM surface is covered by the test substance. Immediately after the test substance is applied, observe the CAM reaction for 5 minutes, noting changes in toxic effects. Record the time of onset of vascular bleeding, coagulation, and angiolysis within 300 seconds, and calculate the irritation score (IS) and classification. Each treatment is repeated 6 times. IS < 1 indicates no irritation; 1 ≤ IS < 5 indicates mild irritation; 5 ≤ IS < 9 indicates moderate irritation; IS > 9 indicates severe irritation. The calculation formula is as follows:

[0034] In the formula: sec H (bleeding time) - the average time it takes for bleeding to begin to occur as observed on the CAM membrane, in seconds (s). sec L (vascular dissolution) - The average time for the onset of vascular dissolution observed on the CAM membrane, in seconds (s); sec C (clotting time) - the average time for the onset of clotting observed on the CAM membrane, measured in seconds (s).

[0035] The test results are shown in Table 2.

[0036] Table 2. Test results of chicken embryo allantoic membrane stimulation test in Example 1

[0037] Test 3: Antioxidant determination of compound essential oils (1) Determination of ABTS free radical scavenging ability The ABTS scavenging rate was determined using a micro-method. 200 μL of ABTS working solution (prepared as a 1:1 mixture of ABTS stock solution and oxidant, stored at room temperature in the dark for 12 hours before measurement; stable for 2-3 days; diluted 50 times with PBS to achieve an absorbance of 0.7 ± 0.05 at 734 nm) was added to 10 μL of sample. After mixing and incubation at room temperature in the dark for 5 minutes, the absorbance was measured at 734 nm and recorded as At. Distilled water was used as a blank control and recorded as A0. 200 μL of PBS solution was reacted with 10 μL of sample in the same manner, and the absorbance was recorded as B. The ABTS free radical scavenging rate was calculated using the following formula, reflecting the strength of the ABTS free radical scavenging ability.

[0038]

[0039] Table 3. Scavenging rate of ABTS free radicals in Example 1

[0040] As shown in Table 3, within a certain range, the scavenging rate of the compound essential oil in Example 1 against ABTS increased with increasing concentration. The scavenging effect of the compound essential oil in Example 1 against ABTS reached its maximum at 5 mg / mL, with a scavenging rate of 23.4%, indicating strong antioxidant capacity.

[0041] (2) Determination of DPPH free radical scavenging ability Take 2 mL of the sample solution diluted to a certain factor and transfer it to a 10 mL test tube. Add 2 mL of DPPH solution (0.1 mmol / L) and react at room temperature for 30 min. Measure the absorbance at 517 nm as A. S Take 2 mL of water and 2 mL of DPPH solution and react them in the same way, recording the absorbance as A0. Take 2 mL of the sample and 2 mL of ethanol solution and react them in the same way, recording the absorbance as A. C The DPPH free radical scavenging rate is calculated using the following formula, reflecting the strength of DPPH scavenging.

[0042]

[0043] Table 4. Scavenging rate of DPPH free radicals in Example 1

[0044] As shown in Table 4, the compound essential oil of Example 1 exhibits good DPPH free radical scavenging effect, showing a dose-dependent relationship with concentration within the range of 0.31 mg / mL to 5 mg / mL. The DPPH scavenging rate gradually increases with increasing concentration. When the concentration of the floral and fruity sweet-scented compound essential oil of this invention is 5 mg / mL, the DPPH free radical scavenging rate is 9.1%.

[0045] Test 4: Mood Improvement Test of 0.5% Fragrance Blend Essential Oil The floral and fruity sweet fragrance from Example 1 was diluted with fractionated coconut oil to a concentration of 0.5 wt%. It was applied evenly to acupoints on the brain at night, with gentle massage to promote absorption, for a period of 28 days. A total of 30 participants (15 men and 15 women) aged 25-45, who were prone to mood swings such as depression, irritability, and restlessness, were selected. Questionnaires were used to rate the fragrance on a scale of 1 to 5, where 1 indicated no effect and symptoms such as depression, irritability, and restlessness. Higher scores indicated stronger soothing effects. The average score was calculated.

[0046] As shown in Table 5, the subjective emotional experiences of the 30 subjects after use showed improvements in multiple dimensions, including improved feelings of physical and mental well-being, emotional stability, slower breathing, and earlier sleep. This indicates that the use of floral and fruity sweet essential oils not only had a positive impact on the subjects' emotional state but may also have improved their physiological state.

[0047] Table 5 Changes in Subjective Emotional Feelings

[0048] Example 4: Preparation of multi-effect skin care essence containing compound essential oils. Table 6 shows the formula of the multi-effect skin care essence containing compound essential oils.

[0049] Table 6. Formulas of Multi-Effect Skin Care Serums Containing Compound Essential Oils

[0050] Example 4: The preparation method of the multi-effect serum containing compound essential oils includes the following steps: Step 1) Accurately weigh the raw materials such as aqueous phase, oil phase, pH adjuster, active ingredient, and fragrance according to the formula, and place them in sterilized and dry containers for later use; Step 2) Prepare suitable production equipment, such as an emulsifying pot with stirring and heating functions, a thermometer, and a pH meter; Step 3) Phase A: Pour deionized water into the emulsifying pot and stir slowly. Add butanediol, 1,3-propanediol, 1,2-pentanediol, glycerol, acrylates / C10-30 alkanol acrylate crosspolymer, allantoin, sodium hyaluronate, polyacrylate crosspolymer-6, betaine and maltodextrin glucoside, and EDTA-2NA in the order of the formula. Start the heating function and stir continuously until the solution is uniform and clear, so that the components are deeply integrated. Raise the temperature of the emulsifying pot to 85°C and maintain a constant temperature to prepare for subsequent mixing with the oil phase. Step 4) Phase B: In another strictly sterilized sterile container, weigh out the oil phase raw materials such as sodium acrylate copolymer, lecithin, and polydimethylsiloxane according to the precise ratio, and stir for 10-15 minutes to fully disperse the components and form a homogeneous, lump-free oil phase mixture. Then heat the oil phase mixture to the same temperature as the water phase, i.e., 85°C, to ensure that the temperature is consistent when the two phases are mixed, and to avoid the emulsification effect being affected by temperature difference; Step 5) Emulsification process: After the oil phase mixture and the aqueous base liquid have reached the predetermined temperature, slowly pour the oil phase into the aqueous phase, and at the same time turn on the high-speed homogenization function of the emulsification pot to make the oil phase evenly dispersed in the aqueous phase in the form of tiny droplets. Continue the homogenization operation until a fine and stable emulsion system is formed to ensure that the emulsion does not separate or precipitate during storage and use. Step 6) Cooling and Adding Active Ingredients, Fragrances, and pH Adjusters in Phase C: After emulsification, turn off the heating device and allow the emulsion to cool while continuously stirring. When the temperature drops to 40℃-45℃, add 1,2-hexanediol, glycerol, citric acid, sodium citrate, tetrahydromethylpyrimidine carboxylic acid, dermal peptide, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5, saccharide isomers, *Salvia frescoeflora* extract, *Edelweiss* flower / leaf extract, panthenol, and other mixtures in sequence. Finally, add the compound essential oil and continue stirring for 10-15 minutes. Step 7) pH Adjustment and Quality Testing: Use a calibrated pH meter to accurately test the multi-effect skincare essence, ensuring its pH value is stably maintained within the suitable range of 4.0-8.0. If the pH value deviates from the standard range, add an appropriate amount of pH adjuster precisely according to the professional formula for fine-tuning. After completing the pH adjustment, conduct a comprehensive quality test on the finished product, covering multiple dimensions such as appearance, odor, viscosity, and microbial indicators. Step 8) Filling and Packaging: The qualified essence is aseptically filled into pre-sterilized packaging bottles. After filling, the bottles are immediately sealed, labeled, and boxed to ensure the product maintains stable quality during transportation and storage.

[0051] Test 5: Stability Evaluation of Multi-Effect Serum Containing Compound Essential Oils The cold and heat stability test was conducted on the multi-effect essence containing compound essential oils according to the experimental methods described in GB / T29665-2013.

[0052] Thermal stability: Place the test sample in two stoppered test tubes. Place one test tube in a constant temperature incubator at 40℃ for 24 h, then remove it and allow it to return to room temperature. Place the other test tube at room temperature for 24 h. Observe whether the sample properties in the two test tubes are consistent and whether the sample separates into layers after heating.

[0053] Cold stability: Place the sample to be tested in a refrigerator at -8℃ for 24 hours, take it out and let it reach room temperature, and compare it with the sample placed at room temperature to observe whether the sample separates into layers.

[0054] Centrifugation test: 2000 r / min, 30 min (except for samples with added insoluble particles or powders), observe whether the sample separates into layers.

[0055] The stability test results are shown in Table 7.

[0056] Table 7. Stability Test Results of Multi-Effect Serums Containing Compound Essential Oils

[0057] Test 6: Microbiological Indicator Requirements The multi-effect essence containing compound essential oils obtained in Example 4 was subjected to hygiene testing according to the "Cosmetic Safety Technical Specifications" (2015 edition). The test results are shown in Table 8 below: Table 8 Test Results

[0058] Test 7: Skin Irritation Test 0.022±0.001 mL of the multi-effect essence containing compound essential oil obtained in Example 4 was placed into the small chamber of the patch applicator. The patch applicator containing the test substance was applied to the inner side of the subject's arm using hypoallergenic adhesive tape. The patch was then gently pressed with the palm of the hand to ensure even application to the skin for 24 hours. Observations were made at 30 min (after the indentation disappeared), 24 h, and 48 h after the patch applicator was removed. The test area was observed and the results were recorded according to the "Skin Reaction Grading Standard for Closed Patch Tests" in the "Cosmetic Safety Technical Specifications (2015 Edition)". Referring to the evaluation criteria of the "Cosmetic Safety Technical Specifications (2015 Edition)" (see Table 9), if more than 5 out of 30 subjects experienced Grade 1 adverse skin reaction, or more than 2 out of 30 subjects experienced Grade 2 adverse skin reaction, or if any one subject experienced Grade 3 or higher adverse skin reaction, the test substance was deemed to have an adverse skin reaction in humans.

[0059] The test results are shown in Table 10. During the experiment, no positive reactions such as erythema, papules, or edema were observed in the patch areas of the 30 subjects. In other words, no adverse irritation reactions were observed in the patch experiment. This indicates that the multi-effect essence containing compound essential oils described in this invention is non-irritating and non-sensitizing to the human body and has good safety.

[0060] Table 9. Grading Criteria for Skin Reactions in Occlusive Patch Tests

[0061] Table 10 Patch Test Results

[0062] Test 8: Soothe Emotions Facial micro-expressions reflect people's emotions, thoughts, and feelings. Facial Action Coding (FACS) systems are currently widely used to describe and analyze the emotions and expressions generated by human facial muscle movements. FACS breaks down facial expressions into basic action units, or AUs. AU combinations map to six basic emotions (happiness, surprise, anger, disgust, sadness, and fear), for example: happiness: AU6 (cheek lift) + AU12 (pull corners of the mouth); surprise: AU1 (inner eyebrow lift) + AU2 (outer eyebrow lift) + AU25 (lips slightly open); anger: AU4 (eyebrows lower and close) + AU5 (upper eyelid lift) + AU23 (lips tighten); Furthermore, mixed emotions (such as contempt) can be identified through unilateral AUs (such as AU14 unilateral cheek muscle activity).

[0063] Fifteen volunteers applied the multi-effect serum containing compound essential oils obtained in Example 4 in front of a two-way mirror. Video recordings were used to monitor changes in pupil size and facial expressions during the application process. The results showed that 93.3% of the participants liked the serum with its sweet floral and fruity fragrance.

[0064] Test 9: Moisturizing Performance After enrollment, participants cleansed their faces uniformly with the same cleansing product, patted them dry with lint-free absorbent paper towels, and sat quietly for 30 minutes in a laboratory at 21±1℃ and 50±10% RH, without drinking water or beverages, keeping their faces exposed and relaxed. The testing personnel designated measurement areas on the cheeks, with each area measuring 2cm × 2cm. Skin stratum corneum moisture content was tested before and 28 days after product use. Each measurement was performed in triplicate for each area, and the average value was taken. If the test data results conformed to a normal distribution, a paired-samples t-test was conducted between the D28 measurement and D0, with a significance level of α=0.05 (two-tailed test). If they did not conform to a normal distribution, a Wilcoxon signed-rank test was conducted between the D28 measurement and D0, with a significance level of α=0.05 (two-tailed test). A p-value > 0.05 for normality indicated that the data followed a normal distribution, and a paired-samples t-test was used. t For statistical analysis, if the distribution does not follow a normal distribution, the rank-sum test is used. "ns" indicates no significant difference; "*" indicates a significant difference, 0.01 ≤ p < 0.05; "**" indicates a significant difference, 0.001 ≤ p < 0.01; "***" indicates a significant difference, p < 0.001.

[0065] Table 11 shows the test results of the moisturizing efficacy of the multi-effect serum containing compound essential oils obtained in Example 4.

[0066] Table 11 Descriptive statistics of stratum corneum moisture content test values ​​(%)

[0067] Moisturizing efficacy is generally evaluated using skin moisture content. Skin moisture content reflects the skin's hydration status and indicates its immediate moisturizing effect. The higher the value, the greater the skin moisture content and the better the immediate moisturizing effect. As shown in Table 11, in this test population (35 people), after using the sample for 28 days, compared with the baseline D0 value, the stratum corneum moisture content showed a significant increase (p < 0.001), indicating that the multi-effect essence containing compound essential oils obtained in Example 4 has a highly significant long-lasting moisturizing effect.

[0068] Test 10: Skin elasticity and firmness After enrollment, participants cleansed their faces uniformly with the same cleansing product, patted them dry with lint-free absorbent paper towels, and sat quietly for 30 minutes in a laboratory at 21±1℃ and 50±10% RH, during which time they could not drink water or beverages, their faces were exposed, and they remained relaxed. The testing personnel determined the measurement area on the cheekbone. The test area for skin elasticity (R value) and skin firmness (F4 value) was 3cm×3cm. Skin stratum corneum moisture content was tested before product use and 28 days after use. Each measurement was performed in triplicate for each area, and the average value was taken. Data processing and analysis methods were the same as above.

[0069] Tables 12 and 13 show the skin elasticity and firmness efficacy test results of the multi-effect essence containing compound essential oils obtained in Example 4.

[0070] Table 12 Descriptive statistics of R test values ​​for skin elasticity (%)

[0071] Table 13 Descriptive statistics of F4 test values ​​for skin firmness (%)

[0072] A higher R-value, representing skin elasticity, indicates higher skin elasticity. A lower F4-value, representing skin firmness, indicates better resistance to suction (better skin firmness). Tables 12 and 13 show that after 4 weeks of product use, the volunteers' R-value for skin elasticity and F4-value for skin firmness increased and decreased relative to their initial values ​​D0, respectively, with statistical significance. After 4 weeks of product use, the R-value and F4-value improved by 15.0% and 14.1%, respectively, indicating that the multi-effect serum containing compound essential oils obtained in Example 4 has a highly significant effect on firming the skin and improving skin elasticity.

[0073] Test 11: Skin Hemoglobin After enrollment, participants cleansed their faces uniformly with the same cleansing product, patted them dry with lint-free absorbent paper towels, and sat quietly for 30 minutes in a laboratory at 21±1℃ and 50±10% RH, during which time they could not drink water or beverages, their faces were exposed, and they remained relaxed. The testing personnel determined the measurement area on the cheeks; the test area for skin hemoglobin was 6.7 cm × 7.2 cm. Data processing and analysis methods were the same as above. Table 14 shows the skin hemoglobin test results of the multi-effect essence containing compound essential oils obtained in Example 4.

[0074] Table 14. Descriptive Statistics of Average Skin Hemoglobin Test Values ​​(%)

[0075] Heme pattern images were obtained using image acquisition instruments, and the average heme concentration per unit area within the selected region of the image was analyzed using specialized analysis software. This parameter in the software is related to the degree of skin redness; the higher the value, the greater the degree of skin redness. The more severe the redness, the better. In this study population (35 people), after using the sample for 28 days, compared with the baseline D0 value, the average value of skin hemoglobin was significantly reduced (0.001≤p<0.01), indicating that the multi-effect essence containing compound essential oils obtained in Example 4 has the effect of relieving skin redness.

[0076] Test 12: Crow's Feet Width and Depth Test After enrollment, participants cleansed their faces uniformly with the same cleansing product, patted them dry with lint-free absorbent paper towels, and sat quietly for 30 minutes in a laboratory at 21±1℃ and 50±10% RH, during which time they could not drink water or beverages, their faces were exposed, and they remained relaxed. The testing personnel determined the measurement area on the cheeks; the test area for parameters related to crow's feet wrinkles was 6.7cm × 7.2cm. Data processing and analysis methods were the same as above. Table 15 shows the experimental test results of the multi-effect essence containing compound essential oils for crow's feet wrinkles obtained in Example 4.

[0077] Table 15 Descriptive statistics of crow's feet wrinkle depth test values ​​(%)

[0078] Test 13: Subject Self-Evaluation The test sample was the multi-effect essence containing compound essential oils obtained in Example 4. After completing a 28-day trial of the sample, the subjects completed a self-assessment based on their own situation and truthfully filled out a questionnaire related to the sample. The assessment results are shown in Table 16.

[0079] Table 16 Subject self-evaluation satisfaction in Example 4

[0080] In this study population (35 participants), after 28 days of continuous use of the test sample, 100.0% of the participants found the sample easy to apply; 100.0% found the sample easily absorbed; 100.0% found the sample had a pleasant feel on the skin; 100.0% found the sample made their skin soft; 100.0% found the sample improved skin smoothness; 94.3% found the sample increased skin elasticity; 100.0% found the sample tightened their skin; 97.1% found the sample improved crow's feet; 94.3% found the sample improved skin redness; 100.0% found the sample provided long-lasting hydration; 97.1% found the sample improved skin texture; overall, 100.0% of the participants were satisfied with the sample.

[0081] In summary, this invention has multiple effects such as moisturizing, soothing, firming, and anti-wrinkle.

[0082] The embodiments are merely preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A multi-effect skincare essence containing compound essential oils, characterized in that: Includes the following raw materials by weight percentage: Component A: Water 71.5%, Disodium EDTA 0.03%, Butylene Glycol 2%, 1,3-Propane Glycol 5%, 1,2-Pentanediol 1.5%, Glycerin 5.0%, Acrylic Acids / C10-30 Alkyl Acrylate Crosspolymer 0.2%, Allantoin 0.2%, Sodium Hyaluronate 0.05%, Polyacrylate Crosspolymer-6 0.1%, Betaine 1.0%, Maltodextrin Glucoside 0.5%; Component B: Sodium acrylate copolymer 0.375%, lecithin 0.125%, polydimethylsiloxane 2.0%, squalane 2.0%, cetyl ethylhexanoate 4.0%, jojoba oil 2.0%, tocopherol 0.05%, 1,2-hexanediol 0.03%, p-hydroxyacetophenone 0.01%, methyl propylene glycol 0.008%, ethylhexylglycerin 0.003%; Component C: 1,2-Hexanediol 0.03~0.06%, Glycerin 0.2%, Citric Acid 0.04~0.08%, Sodium Citrate 0.001~0.002%, Tetrahydromethylpyrimidine Carboxylic Acid 0.3%, Youthful Peptide 0.3%, Dipeptide Diaminobutyrylbenzylamide Diacetate 0.001~0.002%, Palmitoyl Tripeptide-5 0.002~0.004%, carbohydrate isomers 0.07~0.14%, *Salvia flesheii* extract 0.002~0.004%, *Edelweiss* flower / leaf extract 0.002~0.004%, panthenol 0.7~1.0%, compound essential oil 0.5~1.0%, water 0.071%, aminomethylpropanol 0.1%; the youth-enhancing peptide is composed of the following components by weight percentage: Egyptian blue water 90-95%, 1,2-pentanediol (1-5%), 1,2-hexanediol 1-3%, mannitol 1-3%. The compound essential oil comprises the following ingredients in weight percentages: 0.01-0.2% collagen, 0.001-0.01% water, 0.00001-0.0001% sodium chloride, 0.00001-0.0001% sodium dihydrogen phosphate, and 0.00001-0.0001% sodium hyaluronate.

2. The multi-effect skincare essence containing compound essential oils according to claim 1, characterized in that: The compound essential oil is composed of the following ingredients by weight percentage: 15% lemon peel oil, 40% bergamot fruit oil, 5% osmanthus flower extract, 5% lavender oil, 5% geranium leaf oil, 20% lime peel oil, and 10% North American juniper oil.

3. The method for preparing the multi-effect skin care essence containing compound essential oils as described in claim 1, characterized in that: Includes the following steps: Step 1) Accurately weigh the raw materials of components A, B, and C according to the formula and place them in sterilized and dry containers for later use; Step 2) Prepare suitable production equipment, such as an emulsifying pot with stirring and heating functions, a thermometer, and a pH meter; Step 3) Phase A: Pour deionized water into the emulsifying pot and stir slowly. Add butanediol, 1,3-propanediol, 1,2-pentanediol, glycerol, acrylates / C10-30 alkanol acrylate crosspolymer, allantoin, sodium hyaluronate, polyacrylate crosspolymer-6, betaine and maltodextrin glucoside, and EDTA-2NA in the order of the formula. Start the heating function and stir continuously until the solution is uniform and clear, so that the components are deeply integrated. Raise the temperature of the emulsifying pot to 85°C and maintain a constant temperature to prepare for subsequent mixing with the oil phase. Step 4) Phase B: In another strictly sterilized sterile container, weigh out the oil phase raw materials such as sodium acrylate copolymer, lecithin, and polydimethylsiloxane according to the precise ratio, stir for 10-15 minutes to fully disperse the components and form a homogeneous oil phase mixture without lumps. Then heat the oil phase mixture to the same temperature as the water phase, i.e., 85°C, to ensure that the temperature is consistent when the two phases are mixed, and to avoid the effect of emulsification due to temperature difference. Step 5) Emulsification process: After the oil phase mixture and the aqueous base liquid have reached the predetermined temperature, slowly pour the oil phase into the aqueous phase, and at the same time turn on the high-speed homogenization function of the emulsification pot to make the oil phase evenly dispersed in the aqueous phase in the form of tiny droplets. Continue the homogenization operation until a fine and stable emulsion system is formed to ensure that the emulsion does not separate or precipitate during storage and use. Step 6) Cooling and adding active ingredients, fragrances, and pH adjusters in phase C: After emulsification, turn off the heating device and allow the emulsion to cool while continuously stirring; when the temperature drops to 40℃-45℃, add 1,2-hexanediol, glycerol, citric acid, sodium citrate, tetrahydromethylpyrimidine carboxylic acid, dermal peptide, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5, saccharide isomers, *Salvia frescoeflora* extract, *Edelweiss* flower / leaf extract, panthenol, and other mixtures in sequence, and finally add the compound essential oil, and continue stirring for 10 min-15 min; Step 7) pH adjustment and quality testing: Use a calibrated pH meter to accurately test the multi-effect skin care essence to ensure that its pH value is stably maintained within the suitable range of 4.0-8.0; If the pH value deviates from the standard range, an appropriate amount of pH adjuster is added precisely according to the professional formula for fine-tuning; after pH adjustment is completed, a comprehensive quality test is conducted on the finished product, covering multiple dimensions such as appearance, odor, viscosity, and microbial indicators; Step 8) Filling and Packaging: The qualified essence is aseptically filled into pre-sterilized packaging bottles. After filling, the bottles are immediately sealed, labeled, and boxed to ensure the product maintains stable quality during transportation and storage.

Images