Composition containing 4-butylresorcinol and preparation method thereof

Abstract

The invention discloses a composition containing 4-butylresorcinol as well as a preparation method and application of the composition, and belongs to the technical field of biological fermentation and cosmetic raw materials. The composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate, butanediol, isoprene glycol and water, wherein the composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate; wherein the bacillus subtilis lipopeptide sodium is prepared by fermenting bacillus subtilis H01 and has excellent surface activity, emulsifying property and antibacterial activity. The composition realizes efficient whitening by synergistically inhibiting skin tyrosinase activity and reducing melanin content, is good in component compatibility and high in stability, and can be widely applied to preparation of whitening and skin care products.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of biological fermentation and cosmetic raw materials, and particularly relates to a composition containing 4-butylresorcinol and a preparation method thereof. BACKGROUND

[0002] Skin whitening is one of the core needs in the field of skin care, and the excessive production of skin melanin is the main cause of dark skin and the formation of color spots. Tyrosinase is a key rate-limiting enzyme in the melanin synthesis pathway, and inhibiting the activity of tyrosinase and reducing the production of melanin are the core mechanisms of skin whitening products.

[0003] 4-butylresorcinol, as a highly effective skin whitening active ingredient, has been widely used in skin care products. However, it has the problems of poor solubility and insufficient stability in the formula. When used alone, the whitening effect is easily limited and needs to be combined with synergistic ingredients to improve the efficacy. At the same time, the commonly used surfactants or synergistic ingredients in existing skin care compositions are mostly general raw materials, lacking specific synergistic design for 4-butylresorcinol. Moreover, the emulsifying performance and antibacterial activity of some ingredients are not good, making it difficult to balance the whitening effect and use safety of the product.

[0004] In addition, lipopeptide compounds, as natural surfactants, have good biocompatibility and emulsifying ability. However, their performance depends on the screening of production strains. The lipopeptide components prepared by existing strains have insufficient synergistic whitening and long-term stability, and cannot fully exert the synergistic effect with 4-butylresorcinol, resulting in limited efficacy improvement of existing whitening compositions and difficulty in meeting the needs of consumers for high-efficiency and safe whitening products.

[0005] Therefore, it is of great practical significance to develop a 4-butylresorcinol-containing whitening composition with optimized formula, strong ingredient synergy and simple preparation process. SUMMARY

[0006] In view of the deficiencies of the prior art, the purpose of the present application is to obtain a high-efficiency strain by screening, prepare lipopeptide sodium with excellent emulsifying performance and antibacterial activity, enhance the synergistic effect with 4-butylresorcinol, and at the same time solve the problems of poor solubility and insufficient stability of 4-butylresorcinol in skin care formulas, improve the compatibility of ingredients, and improve the whitening efficacy and use safety of the product.

[0007] To achieve the above-mentioned purpose, the following technical solutions are disclosed: In a first aspect, the present application provides a composition containing 4-butylresorcinol, which is composed of the following components in mass percentage: Bacillus subtilis lipopeptide sodium 1-3%; Stearoyl glutamate sodium 1-5%; 4-butylresorcinol 3-8%; Polyglyceryl-4 oleate 10-20%; Butylene glycol 10-20%; Isopentyldiol 40-45%; Water 14-20%.

[0008] Preferably, the bacillus subtilis lipopeptide sodium is obtained by fermentation production of bacillus subtilis H01; The bacillus subtilis H01 was preserved in Guangdong Microbial Culture Collection Center on July 24, 2025, at 5th floor, No. 59 Building, 100 Xianlie Road, Guangzhou, China, with a preservation number of GDMCC No: 66754.

[0009] Preferably, the preparation method of the bacillus subtilis lipopeptide sodium comprises the following steps: S1-1, inoculate bacillus subtilis H01 into fermentation medium, cultivate at 30-40℃, 10-300rpm oscillation for 48-60h, control the pH value of the fermentation broth between 6.5-7.2 during the fermentation process, after the fermentation is completed, centrifuge to remove the bacterial bodies, collect the fermentation supernatant and sterilize it through a filter membrane with a pore size of ≤0.45μm; S1-2, adjust the pH of the sterilized supernatant to 2.0 with an acid solution, white flocculent precipitate appears in the solution, stand at 4℃ for 24h, centrifuge to separate and collect the precipitate, wash the precipitate with pH 2.0 acid solution for 3 times, dissolve the washed precipitate in sterile water, adjust the pH to 6.5-7.0 with an alkali solution, freeze-dry to obtain the bacillus subtilis lipopeptide sodium.

[0010] Further preferably, in step S1, the fermentation medium contains at least one of carbon source, nitrogen source, inorganic salt, organic salt, vitamin and water.

[0011] Further preferably, in step S2, the acid solution is hydrochloric acid aqueous solution.

[0012] Further preferably, in step S2, the alkali solution is sodium hydroxide aqueous solution.

[0013] In a second aspect, the present application provides the use of the composition of the first aspect in the preparation of skin care products with whitening effect.

[0014] In a third aspect, the present application provides a preparation method of the composition of the first aspect, which comprises the following steps: S2-1, 4-butylresorcinol, sodium stearoyl glutamate and sodium subtilisin were weighed and ground into powder, then the ground raw materials, isopentyl glycol, water and butanediol were put into a reaction container at 30℃, stirred uniformly at a speed of 200 rpm / min, and degraded by ultrasonic wave to obtain a mixture A; S2-2, the polyglyceryl-4 oleate was heated to 70-95℃, then the mixture A was added to the polyglyceryl-4 oleate under stirring at a speed of 200 rpm / min, and homogenized and emulsified under a pressure of 1000 bar to obtain the composition.

[0015] Advantages of the present application: 1, the sodium subtilisin obtained by fermentation of Bacillus subtilis H01 and 4-butylresorcinol form a synergistic effect, which can significantly inhibit tyrosinase activity and skin melanin production, and the whitening effect is much better than that of compositions using other strain lipopeptide sodium; 2, the ingredients such as sodium subtilisin, polyglyceryl-4 oleate and isopentyl glycol in the formula provided by the present application effectively improve the solubility of 4-butylresorcinol, and the homogenization and emulsification process ensures that the composition system is stable and not easy to separate and precipitate. DETAILED DESCRIPTION

[0016] Those skilled in the art can improve the process parameters as appropriate based on the content herein. It should be particularly pointed out that all similar substitutions and changes are obvious to those skilled in the art, and they are all considered to be included in the present application.

[0017] The products and methods of the present application have been described by preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the products and methods described herein without departing from the content, spirit and scope of the present application, to realize and apply the present application technology.

[0018] The experimental methods in the following examples not specified in the specific conditions are usually carried out according to the conventional conditions, or according to the conditions recommended by the manufacturer. Unless otherwise specified, the materials, reagents, etc. used in the present application can be obtained from commercial channels. Unless otherwise specified, the substances described in the present application are calculated according to mass percentage and mass fraction.

[0019] In the present application Bacillus subtilis CGMCC No. 1107: purchased from China General Microbial Culture Collection Center; Bacillus subtilis CICC No. 23659: purchased from China Industrial Microbial Culture Collection Center; 1 Screening and isolation of strains 1.1 Preliminary screening and isolation of strains ① Sampling: Collecting soil from the plant garden of Shengzhizyan (Shenzhen) Biotechnology Co., Ltd., taking 5 g of soil sample, adding 45 mL of sterile normal saline, oscillating for 30 min to obtain bacterial suspension, heating the bacterial suspension in water bath at 80℃ for 20 min, and cooling for standby; ② Enrichment culture: inoculating the bacterial suspension into enrichment medium (glucose 25 g / L, peptone 12 g / L, yeast extract 8 g / L, sodium chloride 8 g / L, potassium dihydrogen phosphate 1.5 g / L, magnesium sulfate 0.8 g / L, pH 7.0), and culturing at 37℃, 200 rpm for 48 h; ③ Plate separation: taking the bacterial liquid after enrichment culture, performing 10 -1 to 10 -6 gradient dilution, taking 0.1 mL of 10 -4 , 10 -5 , 10 -6 dilution liquid to coat on blood agar medium (Huankai Biology, item number 024070) plate, and culturing at 37℃ for 48 h, picking the colonies with obvious hemolytic ring for further purification culture; ④ Purification culture: inoculating the picked single colony into LB solid medium (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 10 g / L, agar 15 g / L, pH 7.0) plate for purification culture at 37℃ for 24 h, repeating 4 times to obtain pure strain; ⑤ Preliminary screening and identification: inoculating the pure strain into enrichment medium, and culturing at 37℃, 200 rpm for 60 h, centrifuging the obtained fermentation liquid to collect fermentation supernatant for standby, taking a clean culture dish, adding a layer of water, then adding a drop of edible oil at the center of the water surface, taking a drop of fermentation supernatant and dropping it at the center of the oil film, repeating 3 times for each treatment, and observing: if containing surfactant, the oil film will be pushed away, forming a transparent circle, i.e. oil displacement ring, and the larger the circle diameter, the stronger the surface activity.

[0020] According to the above separation method and oil displacement performance (the oil displacement rings of 5 strains are shown in Table 1), 5 strains are obtained, which are named as H01, H02, H03, H04 and H05 respectively, then performing slant inoculation, and storing in 4℃ refrigerator for standby.

[0021] Table 1 Oil displacement ring diameter (unit: mm) Strain code Oil circle diameter / mm H01 30.33 H02 23.23 H03 21.80 H04 32.53 H05 16.07 1.2 Rescreening and identification of strains 1.2.1 Rescreening (1) Preparation of crude lipopeptide sodium The 5 isolated strains were inoculated into the enrichment medium, and cultured at 37°C and 200 rpm for 60 h. The pH value of the fermentation broth was controlled between 6.5 and 7.2 during the fermentation. After the fermentation, the fermentation broth was centrifuged at 10000 r / min for 15 min to remove the bacteria, the supernatant was sterilized by passing through a filter membrane with a pore size of 0.22 μm, and the supernatant was stored at 4°C for standby; The supernatant after sterilization was adjusted to pH 2.0 with 6 mol / L HCl, and white flocculent precipitate appeared in the solution. The solution was placed at 4°C overnight. The precipitate was collected by centrifugation at 10000 r / min for 20 min, and the precipitate was washed with HCl at pH 2.0 for 3 times. The washed precipitate was dissolved in sterile water, the pH was adjusted to 7.0 with NaOH, and the crude lipopeptide sodium product was obtained by freeze-drying.

[0022] (2) Emulsifying property determination Accurately weigh 20 mg of the crude lipopeptide sodium product of each strain, and dissolve in 20 mL of sterile water to prepare a crude lipopeptide sodium solution of 1000 mg / L. Take a graduated test tube, add 2 mL of kerosene and 2 mL of the crude lipopeptide solution of the corresponding strain, shake and mix uniformly for 2 min, stand for 24 h, measure the emulsion layer height and the total solution height, repeat each treatment 3 times, and calculate the emulsification index according to the following formula: Emulsification index (R 24 , %) = emulsion layer height / total solution height × 100% (3) Emulsion stability determination The emulsion liquid after shaking and mixing was placed for 96 h, and the emulsification index was measured every 24 h. Each treatment was repeated 3 times, and the emulsion stability was calculated according to the following formula: Emulsion stability = R T / R0 × 100% Wherein, R0 is the emulsification index of the solution at 0 h, and R T is the emulsification index of the solution at t h. ① Test strain preparation: Staphylococcus aureus (Guangdong Microbial Culture Collection Center, accession number GDMCC1.160) was inoculated into LB liquid medium, and cultured at 37°C and 200 rpm for 12 h. The concentration of the bacterial suspension was adjusted to 10 6 -10 7 CFU / mL for standby; ② Sterile lipopeptide solution preparation: the crude lipopeptide solution of each strain at 1000 mg / L was sterilized by passing through a 0.22 μm filter membrane to obtain a sterile lipopeptide solution; ③ Plate preparation and inoculation: LB solid medium was poured into a culture dish, and 1 mL of Staphylococcus aureus bacterial suspension was uniformly sprayed after solidification, and the bacterial liquid was absorbed after standing for 5 min; (4) Oxford cup placement and sample addition: Use sterile tweezers to place the Oxford cup on the surface of the plate, 3 for each plate, add 0.2 mL of sterile lipopeptide solution, use sterile water as blank control, each treatment is repeated 3 times; (5) Culture and measurement: Place the plate in a 37°C constant temperature incubator for 24 h, measure the diameter of the inhibition zone around the Oxford cup, record the data.

[0023] (5) Analysis of rescreening results The results of the emulsifying properties and stability of the crude lipopeptide sodium of each strain are shown in Table 2: Table 2 Results of emulsifying properties, stability and antibacterial properties Strain code Emulsification index (R 24 , %) 96h emulsion stability (%) Inhibition zone diameter (mm) H01 78.3±1.2 89.5±0.8 15.67±0.21 H02 62.5±1.5 72.3±1.1 10.33±0.15 H03 58.7±0.9 68.2±0.7 12.47±0.12 H04 70.2±1.3 80.1±0.9 9.50±0.18 H05 45.3±1.1 55.6±1.0 7.20±0.09 According to the results, the crude lipopeptide of H01 strain is significantly better than the other four strains in terms of emulsifying index, 96h emulsifying stability and inhibition zone diameter against Staphylococcus aureus. Combined with the data of oil displacement ring diameter in the preliminary screening, the performance of surface activity, emulsifying properties and antibacterial activity is the best.

[0024] 1.2.2 Identification Morphological properties of strain H01: identified by Gram staining method as Gram-positive bacteria, cells mainly in straight or slightly curved rod shape, blunt round morphology structure at both ends, paired or short chain arrangement, peritrichous flagella, endospore. The growth colonies on the culture medium plate are irregular round, spreading, edge irregular, milky white, after 48h.

[0025] Molecular biology method identification: the 16S rRNA sequence of the strain is shown as SEQ ID NO. 1, as follows: The sequencing results were subjected to BLAST comparison, homology analysis in the NCBI database, and a phylogenetic tree was constructed. Sequence alignment results showed that the 16S rRNA sequence of the strain was highly homologous to the Bacillus subtilis gene sequence (homology > 99%), and combined with its morphological characteristics, H01 was determined to be Bacillus subtilis, and was classified and named as: Bacillus subtilis.

[0026] Bacillus subtilis H01 was preserved in the Guangdong Microbial Culture Collection Center on July 24, 2025, located at No. 59, Building 5, 100, Martyrs' Road, Guangzhou, China, with a postal code of 510070, and a preservation number of GDMCC No: 66754.

[0027] 2 Preparation of a composition containing 4-butylresorcinol 2.1 Preparation of compositions 1-3 The mass percentage of raw materials in the compositions 1-3 is shown in Table 3, wherein the Bacillus subtilis lipopeptide sodium ① is obtained by fermentation of the above-mentioned Bacillus subtilis H01, and the specific preparation method is shown in "1.2.1 (1) Preparation of crude lipopeptide sodium in rescreening"; Table 3 Mass percentage of raw materials of the composition (unit: %) Raw material name Composition 1 Composition 2 Composition 3 Sodium iturin ① 1 2 3 Sodium stearoyl glutamate 1 3 5 4-butyl resorcinol 3 5 8 Polyglyceryl-4 oleate 10 15 20 Butylene glycol 20 15 10 Isopentyldiol 45 43 40 Deionized water Supplemented to 100 Supplemented to 100 Supplemented to 100 The specific preparation steps are as follows: S1, according to Table 3, weigh 4-butylresorcinol, sodium stearoyl glutamate and Bacillus subtilis lipopeptide sodium ①, grind into powder, then at 30℃, put the ground raw materials, isopentyldiol, water and butanediol into a reaction container, stir uniformly at 200 rpm / min, and degrade by ultrasonic wave to obtain mixture A; S2, heat the polyglyceryl-4 oleate to 70-95℃, then add mixture A into the polyglyceryl-4 oleate under stirring at 200 rpm / min, homogenize and emulsify under 1000 bar pressure to obtain the composition containing 4-butylresorcinol.

[0028] 2.2 Preparation of comparative compositions 1-5 The mass percentage of raw materials in the comparative compositions 1-5 is shown in Table 4, and the preparation of the comparative compositions 1-5 is the same as that of the compositions 1-3, wherein the lipopeptide sodium ② is obtained by fermentation of the above-mentioned strain H02, the lipopeptide sodium ③ is obtained by fermentation of the above-mentioned strain H04, the lipopeptide sodium ④ is obtained by fermentation of Bacillus subtilis CGMCC No. 1107, and the lipopeptide sodium ⑤ is obtained by fermentation of Bacillus subtilis CICC No. 23659. The fermentation methods of lipopeptide sodium ②-⑤ are also shown in "1.2.1 (1) Preparation of crude lipopeptide sodium in rescreening". Table 4 Raw material mass percentage of comparative composition (unit: %) Raw material name Comparative composition 1 Comparative composition 2 Comparative composition 3 Comparative composition 4 Sodium lipopeptide ② 2 / / / Sodium lipopeptide ③ / 2 / / Sodium lipopeptide ④ / / 2 / Sodium lipopeptide ⑤ / / / 2 Sodium stearoyl glutamate 3 3 3 3 4-butyl resorcinol 5 5 5 5 Polyglyceryl-4 oleate 15 15 15 15 Butylene glycol 15 15 15 15 Isopentyldiol 43 43 43 43 Deionized water Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Note: " / " in the table means no addition.

[0029] 3 Performance test 3.1 Whitening efficacy test 3.1.1 Purpose of test To evaluate the inhibitory effect of target compositions containing 4-butylresorcinol (compositions 1-3) and comparative compositions (comparative compositions 1-4) on C57BL / 6 mouse skin melanin production and the influence of tyrosinase activity, and to determine the differences in their whitening efficacy.

[0030] 3.1.2 Experimental materials Experimental animals: 32 clean 6-8 week old C57BL / 6 healthy mice, weighing 18-22g, reared in an environment with a temperature of 22-25℃ and a humidity of 50%-60%, with a 12h light-dark cycle and free access to food and water.

[0031] Experimental samples: target compositions (composition 1, composition 2, composition 3), comparative compositions (comparative composition 1, comparative composition 2, comparative composition 3, comparative composition 4), PBS buffer solution (blank control, pH 7.4).

[0032] Reagents and instruments: melanin content detection kit, tyrosinase activity detection kit, tissue homogenizer, spectrophotometer, electronic analytical balance.

[0033] 3.1.3 Experimental grouping and treatment Grouping: After 3 days of adaptive feeding, 32 C57BL / 6 mice were randomly divided into 8 groups, 4 mice in each group, and the grouping is as follows: Blank control group: PBS buffer solution Target composition group: composition 1, composition 2, composition 3 Comparative composition group: comparative composition 1, comparative composition 2, comparative composition 3, comparative composition 4 Treatment: Mouse back depilation: Use an electric shaver to depilate an area of about 2cm x 2cm on the back of the mouse, ensuring that the skin is not damaged, and adapt for 1 day after depilation.

[0034] Sample application: At 9:00 am every day, use a sterile cotton swab to dip the corresponding sample and evenly apply it to the depilated area on the back, with an application amount of 0.2mL per mouse. Apply continuously for 3 weeks, and observe the mouse skin condition (no redness, itching, etc.) every day during this period.

[0035] 3.1.4 Detection indicators and methods 3.1.4.1 Sample processing After 3 weeks of application, the mice were sacrificed by cervical dislocation, and the skin tissue (about 0.5 g per mouse) in the depilated area of the back was quickly removed, washed with pre-cooled PBS for 3 times, and the subcutaneous fat and connective tissue were removed. The skin tissue was cut into small pieces, and pre-cooled PBS was added at a mass-volume ratio of 1:9. The tissue was homogenized in an ice bath using a tissue homogenizer (3000 r / min, 30 s x 3 times) to prepare a 10% tissue homogenate. The homogenate was centrifuged at 10000 r / min for 15 min at 4°C, and the supernatant was collected for use.

[0036] 3.1.4.2 Melanin content determination According to the kit instructions, 0.2 mL of the supernatant was added to 0.8 mL of melanin extraction solution, vortexed, and incubated in a 60°C water bath for 30 min, with mixing every 10 min. After 20 min of centrifugation at 12000 r / min and 4°C, the supernatant was discarded, and the precipitate was washed with anhydrous ethanol for 3 times until the supernatant was colorless. 0.5 mL of 1 mol / L NaOH (containing 10% DMSO) was added to the precipitate, and it was dissolved at 80°C for 30 min. The absorbance value (A475) was measured at 475 nm using a spectrophotometer, and the melanin content was calculated according to the standard curve (unit: μg / mg tissue).

[0037] 3.1.4.3 Tyrosinase activity determination According to the kit instructions, 0.1 mL of the supernatant was added to 0.2 mL of substrate solution (L-tyrosine) in the kit, and incubated in a 37°C water bath for 30 min. Then, 0.1 mL of stop solution was added, mixed, and the absorbance value (A492) was measured at 492 nm using a spectrophotometer. The tyrosinase activity was calculated according to the standard curve (unit: U / mg protein, defined as: the amount of enzyme required to catalyze the generation of 1 μmol of dopaquinone per minute at 37°C).

[0038] 3.1.5 Test results Table 5: Results of melanin content and tyrosinase activity detection in mouse skin (x ± s, n = 4) Group Melanin content (μg / mg tissue) Melanin inhibition rate (%) Tyrosinase activity (U / mg protein) Tyrosinase inhibition rate (%) Blank control (PBS) 12.68±0.95 - 8.35±0.52 - Composition 1 8.92±0.51** 29.65 5.82±0.38** 30.30 Composition 2 6.75±0.43** 46.77 4.15±0.29** 50.30 Composition 3 5.21±0.38** 58.91 3.28±0.25** 60.72 Comparative composition 1 10.85±0.62* 14.43 7.12±0.45* 14.73 Comparative composition 2 11.23±1.08 11.44 7.36±0.49 11.86 Comparative composition 3 10.52±0.99* 17.03 6.98±0.42* 16.41 Comparative composition 4 7.96±0.55** 37.22 5.85±0.40** 29.94 Note: * indicates a significant difference compared with the blank control group, P < 0.05, ** < 0.01; melanin inhibition rate = (melanin content of blank group - melanin content of experimental group) / melanin content of blank group x 100%; tyrosinase inhibition rate = (tyrosinase activity of blank group - tyrosinase activity of experimental group) / tyrosinase activity of blank group x 100%.

[0039] 3.1.6 Results analysis According to Table 5, compared with the blank group, the compositions 1, 2 and 3 can significantly reduce the melanin content in the skin of mice, and the inhibition rate increases with the increase of 4-butylresorcinol content, indicating that 4-butylresorcinol is the core component for inhibiting melanin production.

[0040] Compared with the blank group, the melanin inhibition rate of the comparative compositions 1-4 is 11.44%-37.22%, indicating that the lipopeptide sodium produced by the non-H01 strain has a significantly weaker inhibitory effect on melanin than the lipopeptide sodium from the H01 strain.

[0041] Compared with the blank group, the compositions 1-3 can significantly reduce the tyrosinase activity in the skin of mice, reaching 30.3-60.72%, indicating that the target compositions can play a whitening role by inhibiting the activity of tyrosinase.

[0042] Compared with the blank group, the tyrosinase inhibition rate of the comparative compositions 1-4 is 11.86%-29.94%, which is weaker than the lipopeptide sodium from the H01 strain, verifying that the lipopeptide sodium from the H01 strain and 4-butylresorcinol have a better mutual promotion effect in inhibiting tyrosinase, which is superior to the lipopeptide sodium from other strains.

[0043] In summary, the target composition containing the lipopeptide sodium from the H01 strain of Bacillus subtilis can achieve the whitening effect by significantly inhibiting the activity of skin tyrosinase and reducing the content of melanin, and the effect is better than that of the comparative composition using the lipopeptide sodium from other strains.

[0044] Finally, it should be noted that the above description is only a preferred embodiment of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A composition comprising 4-butylresorcinol, characterized in that, The composition is composed of the following components in mass percentage: Sodium iturin 1-3%; Sodium stearoyl glutamate 1-5%; 4-Butylresorcinol 3-8%; Polyglyceryl-4 oleate 10-20%; Butylene glycol 10-20%; Isopentyldiol 40-45%; Water 14-20%; The sodium iturin is obtained by fermentation of Bacillus subtilis H01; The Bacillus subtilis H01 is preserved in Guangdong Microbial Culture Collection Center on July 24, 2025, at 5th floor, No. 59, Building, 100, Martyrs' Road, Guangzhou, China, with the preservation number of GDMCC No: 66754.

2. The composition of claim 1, wherein, The preparation method of the sodium iturin comprises the following steps: S1-1, inoculate Bacillus subtilis H01 into a fermentation medium, and culture at 30-40℃ and 10-300rpm for 48-60h, control the pH value of the fermentation liquid between 6.5-7.2 during the fermentation process, after the fermentation is completed, centrifuge to remove the bacterial bodies, collect the fermentation supernatant, and sterilize by filtering through a filter membrane with a pore size of ≤0.45μm; S1-2, adjust the pH of the sterilized supernatant to 2.0 with an acid solution, white flocculent precipitate appears in the solution, stand at 4℃ for 24h, centrifuge to separate and collect the precipitate, wash the precipitate with an acid solution with pH 2.0 for 3 times, dissolve the washed precipitate in sterile water, adjust the pH to 6.5-7.0 with an alkali solution, and freeze-dry to obtain the sodium iturin.

3. The composition of claim 2, wherein, In step S1, the fermentation medium contains at least one of carbon source, nitrogen source, inorganic salt, organic salt, vitamin and water.

4. The composition of claim 2, wherein, In step S2, the acid solution is hydrochloric acid aqueous solution.

5. The composition of claim 2, wherein, In step S2, the alkali solution is sodium hydroxide aqueous solution.

6. Use of the composition of any one of claims 1-5 in the preparation of skin care products with whitening effect.

7. A process for the preparation of a composition according to any one of claims 1 to 5, characterized in that The preparation method comprises the following steps: S2-1, weigh 4-butylresorcinol, sodium stearoyl glutamate and sodium iturin, grind into powder, then at 30℃, put the ground raw materials, isopentyldiol, water and butylene glycol into a reaction container, stir uniformly at 200rpm / min, and degrade by ultrasonic wave to obtain mixture A; S2-2, heat polyglyceryl-4 oleate to 70-95℃, then add mixture A into polyglyceryl-4 oleate under stirring at 200rpm / min, homogenize and emulsify under 1000bar pressure to obtain the composition.