An oil control anti-dandruff composition, use and hair product

Abstract

The application belongs to the technical field of washing and protecting products, and discloses an oil-controlling and dandruff-removing composition, application and hair product.The oil-controlling and dandruff-removing composition comprises a Lysidice racemosa extract and a persimmon leaf extract, wherein the Lysidice racemosa extract is obtained by extracting 0.5-5 parts of Lysidice racemosa, the persimmon leaf extract is obtained by extracting 0.5-10 parts of persimmon leaves, and the camellia flower extract is obtained by extracting 0.8-12 parts of camellia flowers.The application provides an oil-controlling and dandruff-removing composition, which can effectively improve the oil-controlling and dandruff-removing effects of the composition by using the Lysidice racemosa extract and the persimmon leaf extract, so as to achieve the oil-controlling and dandruff-removing effects.

Description

Technical Field

[0001] This invention relates to the field of hair care product technology, specifically to an oil-controlling and dandruff-removing composition, its uses, and hair products. Background Technology

[0002] Oil control and dandruff removal are core requirements for maintaining scalp health, and their necessity is reflected in two aspects:

[0003] (1) Excess oil will disrupt the microecological balance of the scalp, providing a breeding ground for Malassezia. The metabolic products of this fungus will stimulate the scalp and trigger an inflammatory response, leading to abnormal shedding of the stratum corneum and the formation of dandruff.

[0004] (2) Long-term untreated oily dandruff may develop into seborrheic dermatitis, manifested as erythema, itching and even hair follicle atrophy.

[0005] Therefore, many shampoos and conditioners have added oil-controlling and dandruff-reducing ingredients to achieve excellent oil-controlling and dandruff-reducing effects. Years ago, shampoos and conditioners generally used stronger oil-controlling and dandruff-reducing ingredients to achieve this effect, such as zinc pyrithione. This was suitable for oily scalps with mild to moderate seborrheic dermatitis or fungal dandruff, and the oil-controlling effect could last for 24-36 hours. It also had some anti-inflammatory properties. However, substances like zinc pyrithione are highly toxic to aquatic organisms and may cause skin allergies and immunosuppression in humans. Therefore, it is necessary to find new ingredients to achieve excellent oil-controlling and dandruff-reducing effects while minimizing other adverse effects on the human body. Summary of the Invention

[0006] One of the objectives of this application is to provide an oil-controlling and dandruff-removing composition, which is formed around persimmon leaf extract, has excellent oil-controlling and dandruff-removing effects, and is derived from plants, thus having the advantage of being mild and safe.

[0007] Another objective of this application is to provide the use of an oil-controlling and dandruff-removing composition, which, when prepared into a shampoo and conditioner product, has excellent oil-controlling and dandruff-removing effects.

[0008] In addition, this application also provides a shampoo and conditioner product that has oil-control and dandruff-removing effects.

[0009] To achieve the above objectives, this application provides an oil-controlling and dandruff-removing composition comprising a kidney tea extract and a persimmon leaf extract. By weight fraction, the kidney tea extract is obtained from 0.5 to 5 parts of kidney tea, and the persimmon leaf extract is obtained from 0.5 to 10 parts of persimmon leaves.

[0010] This application found that the combination of kidney tea extract and persimmon leaf extract has a synergistic effect, and at the same concentration, the combination of the two has a better oil control and Malassezia inhibition effect.

[0011] Preferably, it also includes camellia extract obtained from 0.8 to 12 parts of camellia flowers.

[0012] This application, based on the compound of persimmon leaf extract and kidney tea extract, further adds camellia flower extract, which can significantly improve the oil control and Malassezia inhibition effects of the composition.

[0013] It should be noted that the persimmon leaf extract, kidney tea extract, and camellia flower extract of this application can be obtained from commercially available products, and can also achieve corresponding synergistic effects through compound formulation; more preferably, the persimmon leaf extract, kidney tea extract, and camellia flower extract of this application are prepared using the following method:

[0014] The persimmon leaf extract was obtained by the following steps:

[0015] Step 1: After crushing the persimmon leaves into powder, add 8-15 BV of 70% ethanol solution, stir and extract at 55±5℃ for 2-4 hours, and centrifuge to obtain persimmon leaf extract;

[0016] Step 2: Concentrate the persimmon leaf extract until it is alcohol-free to obtain alcohol-free concentrate A;

[0017] Step 3: Add polyol and water to alcohol-free concentrate A and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of polyol to water is 20-50:30-60, and the polyol accounts for 20-50 wt% of the total weight of alcohol-free concentrate A, polyol and water.

[0018] The kidney tea extract was obtained by the following steps:

[0019] Step a: After crushing the kidney tea into powder, add 10~18 BV of 70% ethanol solution, stir and extract at 50±3℃ for 1~2 hours, and centrifuge to obtain kidney tea extract;

[0020] Step b: Concentrate the kidney tea extract until it is alcohol-free to obtain alcohol-free concentrate B;

[0021] Step c: Add polyol and water to alcohol-free concentrate B and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of polyol to water is 20~50:30~60, and the polyol accounts for 20~50 wt% of the total weight of alcohol-free concentrate B, polyol and water.

[0022] The camellia flower extract was obtained by the following steps:

[0023] Step e: After crushing the camellia flowers into powder, add 12~18 BV of 50% ethanol solution, stir and extract at 45±5℃ for 1~2 hours, and centrifuge to obtain camellia flower extract.

[0024] Step f: Concentrate the camellia extract until it is alcohol-free to obtain alcohol-free concentrate C;

[0025] Step g: Add polyol and water to alcohol-free concentrate C and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of polyol to water is 20~50:30~60, and the polyol accounts for 20~50 wt% of the total weight of alcohol-free concentrate C, polyol and water.

[0026] It should be noted that, in the preparation process of the oil-controlling and dandruff-removing composition of this application, in addition to adding polyol and water to alcohol-free concentrate A, alcohol-free concentrate B, and alcohol-free concentrate C respectively, stirring to dissolve and then performing ultrafiltration to obtain the filtrate; alternatively, any two or more of alcohol-free concentrate A, alcohol-free concentrate B, and alcohol-free concentrate C can be mixed first, and then polyol and water can be added, stirred to dissolve, and then performed ultrafiltration to obtain the filtrate.

[0027] The polyol is at least one of butanediol, glycerol, methylpropanediol, 1,3-propanediol and dipropylene glycol.

[0028] In this invention, BV refers to bed volume, abbreviated as BV. In chemical production, resins are commonly used. The amount of solution added to a resin column is determined by the amount of resin packed in it; 1L of resin passing through 1L of solution is called 1BV. This concept has been extended in the plant extraction industry, typically used to represent the weight of extraction solvent added based on the weight of the raw material. It can also be called a multiple, for example, adding 10kg of water to 1kg of raw material for extraction is recorded as 10BV. This method allows for a clear understanding of the relationship between the amount of raw material and the amount of solvent used in product development and production.

[0029] Furthermore, the specific operation of stirring and dissolving is as follows: stirring at a speed of 600~750 rpm for 0.5~1 h at room temperature; the specific operation of ultrafiltration is as follows: ultrafiltration is performed at an ultrafiltration pressure of 0.20~0.30 MPa, controlling the ultrafiltration temperature to not exceed 35℃, and ultrafiltration membrane pore size to be 0.1~0.45 μm.

[0030] Furthermore, the specific operation of the concentration is as follows: rotary evaporation is performed under the conditions of a temperature of 50~60℃, a pressure of -0.08~-0.1MPa, and a rotation speed of 40~60r / min.

[0031] Preferably, the oil-controlling and dandruff-removing composition further includes a preservative, and the content of the preservative in the oil-controlling and dandruff-removing composition is 0.5~2wt%.

[0032] Furthermore, after adding preservatives to the above-mentioned anti-dandruff composition, it needs to be sterilized at 85°C for 1 hour.

[0033] Furthermore, the preservative is at least one selected from 1,2-hexanediol, p-hydroxyacetophenone, 1,2-pentanediol, octyl glycol, ethylhexylglycerin, and capryloyl hydroxamic acid.

[0034] This application also provides the use of the above-described oil-controlling and dandruff-removing composition in the preparation of shampoo and conditioner products.

[0035] This application also discloses a shampoo and conditioner containing 0.1 to 90 wt% of the above-mentioned oil-controlling and dandruff-removing composition.

[0036] Beneficial effects:

[0037] Compared with the prior art, this application provides an oil-controlling and dandruff-removing composition, which can effectively enhance the oil-controlling and antibacterial effects of the composition through persimmon leaf extract and kidney tea extract, thereby achieving the effect of oil control and dandruff removal. Attached Figure Description

[0038] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0039] Figure 1 This is a picture of a chicken embryo before use in Embodiment 6 of the present invention;

[0040] Figure 2 This is a picture of a chicken embryo after use in Embodiment 6 of the present invention. Detailed Implementation

[0041] The present application will be further described below with reference to embodiments, but this does not constitute any limitation on the present application. Any limited modifications made within the scope of the claims of the present application shall still be within the scope of the claims of the present application.

[0042] To illustrate the technical content of this application in detail, the following description is provided in conjunction with the embodiments.

[0043] In the following examples and comparative examples, the persimmon leaves and kidney tea were purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.; the camellia flowers and sophora flavescens roots were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0044] Unless otherwise specified, in the following embodiments and comparative examples, the parts and % refer to parts by weight and weight percentage, respectively.

[0045] In the following examples and comparative examples, the specific operation of stirring and dissolving is as follows: stirring at 750 rpm for 1 hour at room temperature; the specific operation of ultrafiltration is as follows: ultrafiltration at an ultrafiltration pressure of 0.25 MPa, controlling the ultrafiltration temperature to not exceed 35°C, and ultrafiltration membrane pore size to be 0.2 μm; the specific operation of concentration is as follows: rotary evaporation at a temperature of 55°C, a pressure of -0.1 MPa, and a rotation speed of 50 r / min.

[0046] The composition was prepared according to the formula in Table 1. The preparation method was as follows: the corresponding components (persimmon leaf extract, kidney tea extract, camellia flower extract, and sophora flavescens root extract) were mixed to obtain the finished product.

[0047] The persimmon leaf extract was obtained by the following steps:

[0048] Step 1: After crushing the persimmon leaves into powder, add 12 BV of 70% ethanol solution, stir and extract at 55±5℃ for 3 hours, and centrifuge to obtain persimmon leaf extract;

[0049] Step 2: Concentrate the persimmon leaf extract until it is alcohol-free to obtain alcohol-free concentrate A;

[0050] Step 3: Add butanediol and water to alcohol-free concentrate A and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of butanediol to water is 25:53, and butanediol accounts for 28 wt% of the total weight of alcohol-free concentrate A, butanediol and water.

[0051] The kidney tea extract was obtained by the following steps:

[0052] Step 1: After crushing the kidney tea into powder, add 14 BV of 70% ethanol solution, stir and extract at 50±3℃ for 2 hours, and centrifuge to obtain kidney tea extract;

[0053] Step 2: Concentrate the kidney tea extract until it is alcohol-free to obtain alcohol-free concentrate B;

[0054] Step 3: Add butanediol and water to alcohol-free concentrate B and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of butanediol to water is 25:53, and butanediol accounts for 28 wt% of the total weight of alcohol-free concentrate B, butanediol and water.

[0055] The camellia flower extract was obtained by the following steps:

[0056] Step 1: After crushing the camellia flowers into powder, add 15 BV of 50% ethanol solution, stir and extract at 45±5℃ for 2 hours, and centrifuge to obtain camellia flower extract;

[0057] Step 2: Concentrate the camellia extract until it is alcohol-free to obtain alcohol-free concentrate C;

[0058] Step 3: Add butanediol and water to alcohol-free concentrate C and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of butanediol to water is 25:53, and butanediol accounts for 28 wt% of the total weight of alcohol-free concentrate C, butanediol and water.

[0059] The Sophora flavescens root extract was obtained by the following steps:

[0060] Step 1: After crushing the Sophora flavescens root into powder, add 12 BV of 70% ethanol solution, stir and extract at 55±5℃ for 3 hours, and centrifuge to obtain Sophora flavescens root extract.

[0061] Step 2: Concentrate the Sophora flavescens root extract until it is alcohol-free to obtain alcohol-free concentrate D;

[0062] Step 3: Add butanediol and water to alcohol-free concentrate D and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of butanediol to water is 25:53, and butanediol accounts for 28 wt% of the total weight of alcohol-free concentrate D, butanediol and water.

[0063] Table 1 Formulation Table

[0064] Persimmon leaf extract (per serving) Kidney Tea Extract (per serving) Camellia flower extract (per serving) Sophora flavescens root extract (per serving) Example 1 0.5 5 0 0 Example 2 5 2.5 0 0 Example 3 10 0.5 0 0 Example 4 5 2.5 0.8 0 Example 5 5 2.5 12 0 Example 6 5 1 5 0 Comparative Example 1 5 0 0 0 Comparative Example 2 0 1 0 0 Comparative Example 3 0 0 5 0 Comparative Example 4 0 0 0 5 Comparative Example 5 5 0 5 0 Comparative Example 6 0 1 5 0 Comparative Example 7 5 1 0 5

[0065] Efficacy testing

[0066] I. 5α-Reductase Inhibition Rate

[0067] Test method:

[0068] 1. Experimental Objective and Principle

[0069] Oily skin has overactive sebaceous glands, resulting in a greasy face that is difficult to clean and has an unsightly shine. It is also prone to related skin conditions such as acne vulgaris and seborrheic dermatitis. Given the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of a test sample on 5α-reductase is typically used to assess its sebum-regulating effect and characterize its oil-controlling efficacy.

[0070] 2. Experimental Materials and Methods

[0071] 2.1 Instruments and equipment: BSA224S analytical balance, Agilent 1220 high performance liquid chromatograph.

[0072] 2.2 Reagents:

[0073] Testosterone, ≥98%;

[0074] NADPH, 99%;

[0075] Methanol, HPLC grade;

[0076] Finasteride, ≥98%;

[0077] Buffer solution: PBS (pH=5.5).

[0078] 2.3 Test Methods

[0079] (1) Treatment of control materials and test samples

[0080] Sample group: Dilute with buffer to a sample concentration of 0.5% for the compositions of Examples 1-6 and Comparative Examples 1-7;

[0081] Positive control (finasteride): Dissolve in methanol first, then dilute with buffer to a test concentration of 400 nM;

[0082] Negative control: PBS buffer.

[0083] (2) Experimental operation procedures

[0084] Sample groups, positive control groups, and negative control groups were set up. Different reagent solutions were added to each group, and the mixtures were shaken well. Each group was analyzed by HPLC to determine the testosterone content in each tube.

[0085] (3) Calculation formula

[0086]

[0087] The compositions of Examples 1-6 and Comparative Examples 1-7 were tested according to the above method, and the results are shown in Table 2.

[0088] Table 2. Results of 5α-reductase inhibition rate of the compositions in Examples 1-6 and Comparative Examples 1-7

[0089] Group Average 5α-reductase inhibition rate % Example 1 55.66 Example 2 59.93 Example 3 68.70 Example 4 65.01 Example 5 61.34 Example 6 72.11 Comparative Example 1 37.25 Comparative Example 2 34.29 Comparative Example 3 26.51 Comparative Example 4 30.10 Comparative Example 5 49.98 Comparative Example 6 42.30 Comparative Example 7 46.31 Positive control - Finasteride (400 nM) 60.48

[0090] According to the results in Table 2:

[0091] According to the data from Examples 1-3, this application produces excellent oil-controlling effects through the combination of persimmon leaf extract and kidney tea extract. The 5α-reductase inhibition rate exhibited by the composition is close to that of the positive control, and even Example 3 exceeds the positive control by a large margin. Based on the data pattern, it can be inferred that the higher the concentration of persimmon leaf extract in the compositions of Examples 1-3 of this application, the higher the 5α-reductase inhibition rate. In other words, the compositions of Examples 1-3 of this application are based on persimmon leaf extract.

[0092] Analysis of the data from Examples 4-6 revealed that while Examples 4 and 5 also conformed to the above speculation—that adding camellia extract to the composition to reduce the concentration of persimmon leaf extract significantly decreased the 5α-reductase inhibition rate of the composition, and the greater the decrease in the concentration of persimmon leaf extract, the greater the decrease in the 5α-reductase inhibition rate—Example 6, compared to Examples 1-4, showed a significantly higher 5α-reductase inhibition rate despite having a relatively lower concentration of persimmon leaf extract. This is presumably because the specific ratio of persimmon leaf extract, kidney tea extract, and camellia extract produced a significant synergistic effect, greatly enhancing the 5α-reductase inhibition rate of the composition.

[0093] Based on the data comparison of Example 2 and Comparative Examples 1 and 2, it can be seen that, when used as a single component, the 5α-reductase inhibition rate of persimmon leaf extract is indeed higher than that of kidney tea extract, but the difference is not significant. However, when the two are combined to form a composition, the 5α-reductase inhibition rate is significantly increased. Therefore, it can be inferred that the combination of persimmon leaf extract and kidney tea extract can synergistically enhance the 5α-reductase inhibition rate of the composition. Furthermore, combined with the results of Examples 1-3, this synergistic effect is mainly due to persimmon leaf extract.

[0094] Comparing the data from Examples 1-3, it can be seen that the 5α-reductase inhibition rate of camellia flower extract is weaker than that of persimmon leaf extract and kidney tea extract. The results of Examples 4 and 5 also largely confirm this trend. However, Example 6 showed a significant synergistic effect; two possibilities are speculated:

[0095] (1) The synergistic effect of persimmon leaf extract and kidney tea extract at a specific ratio is excellent;

[0096] (2) The combination of persimmon leaf extract, kidney tea extract and camellia flower extract in a specific ratio produced a significant synergistic effect, which greatly improved the 5α-reductase inhibition rate of the composition.

[0097] To verify the above possibilities, this application verified whether camellia played a role in this combination through Comparative Examples 4 and 7. The results confirmed that Example 6 of this application produced a significant synergistic effect in the combination of persimmon leaf extract, kidney tea extract and camellia extract in a specific ratio, which greatly improved the 5α-reductase inhibition rate of the composition.

[0098] Furthermore, based on the data comparison between Example 6 and Comparative Examples 5 and 6, it can be seen that the significant synergistic effect produced by Example 6 of this application is formed by the combination of persimmon leaf extract, kidney tea extract and camellia flower extract in a specific ratio.

[0099] II. Anti-dandruff efficacy test (inhibition rate of Malassezia furfur)

[0100] Test method:

[0101] 1. Experimental Materials and Methods

[0102] 1.1 Instruments and equipment: biochemical incubator, vertical high-pressure steam sterilizer.

[0103] 1.2 Reagents: Phosphate buffer, Malassezia culture medium, Malassezia.

[0104] 1.3 Experimental Methods

[0105] (1) Treatment of control materials and test samples

[0106] Blank control: Phosphate buffer;

[0107] Control sample group: bacterial carrier (Malassezia suspension) + phosphate buffer.

[0108] 2. Experimental Procedure

[0109] Take a suspension of Malassezia furfur and add it to each sample solution and control solution respectively. Mix well and start timing. After the specified reaction time, perform 10... 1 10 2 10 3 10 4 Four dilutions were performed. The sample solution and the control solution were each placed in two petri dishes, and Malassezia sylvestris culture medium was poured in and mixed thoroughly. After solidification, the plates were inverted and incubated in an incubator for 5-10 days. Viable bacteria were then counted.

[0110] 3. Calculation Formula

[0111] Antibacterial rate = (AB) / A*100%.

[0112] Where: A—average colony count of the control sample;

[0113] B—The average number of colonies in the tested sample.

[0114] Evaluation criteria: If the antibacterial rate is ≥50%~90%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has strong antibacterial effect.

[0115] The oil-controlling and dandruff-removing compositions obtained in Examples 1-6 and Comparative Examples 1-7 were tested according to the above method, with an inhibition time of 10 minutes. The results are shown in Table 3. It should be noted that since the oil-controlling and dandruff-removing compositions can be used in various products, a relatively long inhibition time of 10 minutes was set to confirm the long-lasting antibacterial effect of the compositions.

[0116] Table 3. Test results of the dandruff-removing efficacy of the oil-controlling and dandruff-removing compositions obtained in Examples 1-6 and Comparative Examples 1-7.

[0117] Average colony count (CFU / mL) Malassezia furfur inhibition rate % Example 1 2400 80.00 Example 2 1900 84.17 Example 3 900 92.50 Example 4 1500 87.50 Example 5 1200 90.00 Example 6 190 98.42 Comparative Example 1 5200 56.67 Comparative Example 2 7000 41.67 Comparative Example 3 5400 55.00 Comparative Example 4 6000 50.00 Comparative Example 5 2900 75.83 Comparative Example 6 3800 68.33 Comparative Example 7 3200 73.33 control sample 12000 / Blank control ＜10 /

[0118] According to the results in Table 3:

[0119] According to the data from Examples 1-3, this application produces excellent antibacterial effects through the combination of persimmon leaf extract and kidney tea extract. Based on the data patterns, it can be inferred that the higher the concentration of persimmon leaf extract in the compositions of Examples 1-3 of this application, the higher the inhibition rate of Malassezia furfur. In other words, the compositions of Examples 1-3 of this application are based on persimmon leaf extract.

[0120] However, unlike the 5α-reductase inhibition rate results in Table 2, when camellia extract was added to the composition to reduce the concentration of persimmon leaf extract, the inhibition rate of Malassezia furfur was somewhat reduced. However, in Example 5, with the addition of a high concentration of camellia extract, the inhibition rate of Malassezia furfur was even higher than that in Example 4. In other words, when persimmon leaf extract, kidney tea extract, and camellia extract are combined, the inhibition rate of Malassezia furfur is not based on persimmon leaf extract. Based on the data from Example 6, it can be inferred that the combination of persimmon leaf extract, kidney tea extract, and camellia extract produced a significant synergistic effect, and the change in the ratio also has a certain impact on the synergistic effect of the composition. The optimal ratio is that of Example 6.

[0121] Based on the data comparison of Example 2 and Comparative Examples 1 and 2, it can be seen that, when used as a single component, the inhibition rate of Malassezia furfur by persimmon leaf extract is indeed higher than that of kidney tea extract. However, when the two are combined to form a composition, the 5α-reductase inhibition rate is significantly increased. Therefore, it can be inferred that the combination of persimmon leaf extract and kidney tea extract can synergistically enhance the 5α-reductase inhibition rate of the composition. Furthermore, based on the results of Examples 1-3, this synergistic effect is mainly due to persimmon leaf extract.

[0122] Based on the data comparison of Comparative Examples 1-3, it can be seen that the inhibition rate of Malassezia furfur by Camellia extract is weaker than that of Persimmon leaf extract but higher than that of Kidney tea extract. Therefore, the results of Examples 4 and 5 can be understood. The applicant has reason to believe that the combination of Persimmon leaf extract, Kidney tea extract and Camellia extract produces a synergistic effect while the proportion of each component is in accordance with a certain rule, which can be adjusted by the results of Comparative Examples 1-3.

[0123] However, Example 6 produced a significant synergistic effect; it is speculated that the specific ratio of persimmon leaf extract, kidney tea extract and camellia flower extract produced a significant synergistic effect, which greatly improved the inhibition rate of Malassezia furfur in the composition.

[0124] To verify the above possibilities, this application verified through Comparative Examples 4 and 7 that Example 6 of this application produced a significant synergistic effect by combining persimmon leaf extract, kidney tea extract, and camellia flower extract in a specific ratio, which greatly improved the inhibition rate of Malassezia furfur in the composition; and according to the comparison of the data of Example 6 with Comparative Examples 5 and 6, it can be seen that the significant synergistic effect produced by Example 6 of this application is formed by the combination of persimmon leaf extract, kidney tea extract, and camellia flower extract in a specific ratio.

[0125] III. Irritation Test (Chicken Embryo Villoallantoic Membrane Test)

[0126] Test method:

[0127] 1. Experimental Objective and Principle

[0128] The chorioallantoic membrane (CAM) test is an early and widely used in vitro method for assessing eye irritation. The CAM is a respiratory membrane surrounding the chicken embryo. This test utilizes the intact, clear, and transparent vascular system of the mid-stage chorioallantoic membrane in hatched chicken embryos. A certain amount of the test substance is directly exposed to the CAM, and after a period of time, changes in CAM toxicity indicators (such as hemorrhage, coagulation, and vascularization) are observed. These indicators reflect changes in the morphology, color, and permeability of blood vessels and vascular networks, as well as phenomena such as CAM protein denaturation and the degree of damage. These indicators are then combined to obtain a score used to assess the eye irritation of the test substance.

[0129] The purpose of this study is to test the ability of the test substance to cause toxic changes in the chorioallantoic membrane of chicken embryos and to evaluate the elements and processes of the potential eye irritation of the substance being evaluated.

[0130] 2. Experimental Materials and Methods

[0131] 2.1 Instruments and equipment: fully automatic incubator, stereomicroscope, SPF chicken embryos.

[0132] 2.2 Reagents: Sodium chloride, sodium dodecyl sulfonate (SDS).

[0133] 2.3 Incubation conditions: Room temperature 20~25℃, relative humidity 45~70%. Incubation temperature 37.5±0.5℃, relative humidity 55~70%, turn the tray 3~6 times / h. Rotation is not necessary for 9-day-old chicken embryos during incubation.

[0134] 3. Experimental Procedure

[0135] In this test, 6 chicken embryos were selected for each group. The condition of the chorioallantoic membrane was recorded using a photographic device. The sample to be tested was added to the chorioallantoic membrane of the chicken embryo, the time of sample addition was recorded, and the air cell was covered with a moistened plastic wrap. The chicken embryos were then transferred to a constant temperature and humidity incubator for culture. The degree of change of each toxic effect was observed and photographed.

[0136] 4. Results Observation

[0137] Observe and record bleeding, coagulation, and vascular dissolution, and score them according to their severity.

[0138] 5. Result Judgment Criteria

[0139] ES≤4, non-irritating;

[0140] 4 < ES ≤ 12, mild irritation;

[0141] 12 < ES < 16, moderately irritating;

[0142] ES≥16, highly irritating / corrosive.

[0143] 6. Test Results

[0144] The sample of Example 6 was tested according to the above testing method. ES=2.00, and the result was determined to be non-irritating. The results of chicken embryos before and after use in Example 6 are as follows: Figure 1 , Figure 2 As shown, this demonstrates that the technical solution of the present invention is mild and non-irritating.

[0145] Application Example 1

[0146] An oil-controlling and dandruff-removing product is prepared according to the formula in Table 4. The preparation method is as follows: It should be noted that the sample containing Example 6 is prepared by mixing all raw materials according to the formula in Table 4 and sterilizing at 85±3℃ for 1 hour.

[0147] Table 4 Ingredient list of oil-controlling and dandruff-removing products

[0148] Raw material name Amount added (%) The composition obtained in Example 6 89.27 Capryloyl hydroxamic acid 0.05 Ethylhexylglycerin 0.08 1,2-Hexanediol 0.6 glycerin 10

[0149] Application Example 2

[0150] An oil-controlling and dandruff-reducing shampoo is prepared according to the formula in Table 5. The preparation method is as follows:

[0151] Step 1: Add pure water from phase A to a homogenizing pot, add polyquaternium-10 under homogenization conditions, and after homogenization and even dispersion, heat to 85℃ under stirring conditions, keep warm and stir for 15-20 minutes to obtain phase A;

[0152] Step 2: Maintain 85°C, add phase B raw material, and stir while keeping warm until completely dissolved;

[0153] Step 3: Cool down to 45℃, add the C phase raw material, disperse and dissolve evenly to obtain shampoo.

[0154] Table 5. Oil-control and anti-dandruff shampoo formula table

[0155]

[0156] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. An oil-controlling and dandruff-removing composition, characterized in that, By weight, it includes 0.5 to 5 parts of kidney tea extract, 0.5 to 10 parts of persimmon leaf extract, and 0.8 to 12 parts of camellia flower extract.

2. The oil-controlling and dandruff-removing composition according to claim 1, characterized in that, The persimmon leaf extract was obtained by the following steps: Step 1: After crushing the persimmon leaves into powder, add 8-15 BV of 70% ethanol solution, stir and extract at 55±5℃ for 2-4 hours, and centrifuge to obtain persimmon leaf extract; Step 2: Concentrate the persimmon leaf extract until it is alcohol-free to obtain alcohol-free concentrate A; Step 3: Add polyol and water to alcohol-free concentrate A and stir to dissolve. Then perform ultrafiltration to collect the filtrate. The weight ratio of polyol to water is 20-50:30-60, and the polyol accounts for 20-50 wt% of the total weight of alcohol-free concentrate A, polyol, and water. The kidney tea extract was obtained by the following steps: Step a: After crushing the kidney tea into powder, add 10~18 BV of 70% ethanol solution, stir and extract at 50±3℃ for 1~2 hours, and centrifuge to obtain kidney tea extract; Step b: Concentrate the kidney tea extract until it is alcohol-free to obtain alcohol-free concentrate B; Step c: Add polyol and water to alcohol-free concentrate B and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of polyol to water is 20~50:30~60, and the polyol accounts for 20~50 wt% of the total weight of alcohol-free concentrate B, polyol and water.

3. The oil-controlling and dandruff-removing composition according to claim 1, characterized in that, The camellia flower extract was obtained by the following steps: Step e: After crushing the camellia flowers into powder, add 12~18 BV of 50% ethanol solution, stir and extract at 45±5℃ for 1~2 hours, and centrifuge to obtain camellia flower extract. Step f: Concentrate the camellia extract until it is alcohol-free to obtain alcohol-free concentrate C; Step g: Add polyol and water to alcohol-free concentrate C and stir to dissolve. Then perform ultrafiltration to obtain the filtrate. The weight ratio of polyol to water is 20~50:30~60, and the polyol accounts for 20~50 wt% of the total weight of alcohol-free concentrate C, polyol and water.

4. The oil-controlling and dandruff-removing composition according to claim 2 or 3, characterized in that, The specific operation of stirring and dissolving is as follows: stirring at 600~750 rpm for 0.5~1 h at room temperature; the specific operation of ultrafiltration is as follows: ultrafiltration is performed at an ultrafiltration pressure of 0.20~0.30 MPa, controlling the ultrafiltration temperature to not exceed 35℃, and ultrafiltration membrane pore size to be 0.1~0.45 μm.

5. The oil-controlling and dandruff-removing composition according to claim 2 or 3, characterized in that, The specific operation of concentration is as follows: rotary evaporation is carried out at a temperature of 50~60℃, a pressure of -0.08~-0.1MPa, and a rotation speed of 40~60r / min.

6. The oil-controlling and dandruff-removing composition according to claim 1, characterized in that, The oil-controlling and dandruff-removing composition also includes a preservative, and the content of the preservative in the oil-controlling and dandruff-removing composition is 0.5~2wt%.

7. The oil-controlling and dandruff-removing composition according to claim 6, characterized in that, The preservative is at least one selected from 1,2-hexanediol, p-hydroxyacetophenone, 1,2-pentanediol, octyl glycol, ethylhexylglycerin, and capryloyl hydroxamic acid.

8. Use of the oil-controlling and dandruff-removing composition as described in any one of claims 1 to 7 to prepare personal care products.

9. A hair product, characterized in that, Containing 0.1 to 90 wt% of the oil-controlling and dandruff-removing composition according to any one of claims 1 to 7.

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