Carbon source lactic acid fertilizer for promoting growth of beneficial algae and preparation method thereof

Abstract

The invention provides a carbon source lactic acid fertilizer for promoting growth of beneficial algae and a preparation method thereof, and relates to the field of fertilizers. The carbon source lactic acid fertilizer comprises the following raw materials in parts by weight: 360-400 parts of an organic carbon source, 160-190 parts of a nitrogen source, 20-30 parts of trace elements, 100-130 parts of compound bacteria, 10-15 parts of compound enzyme and 30-45 parts of a compound, the compound bacteria are prepared from lactic acid bacteria, saccharomycetes and bacillus according to the mass ratio of (50 to 60) to (20 to 30) to (35 to 40); the compound enzyme is prepared from beta-1, 4-glucosidase, papain and beta-amylase according to the mass ratio of (8 to 12) to (3 to 5) to (1 to 2); the compound is composed of light calcium carbonate, diatomite and sepiolite powder in a mass ratio of (15-20): (5-10): (10-15). The carbon source lactic acid fertilizer disclosed by the invention can accurately regulate and control an algae community structure, promote the growth of beneficial algae, realize multi-composite micro-ecological purification, quickly improve the culture water quality and synergistically improve the stability of an ecological system, and is green, safe, efficient, durable and wide in application range.

Description

Technical Field

[0001] This invention relates to the field of fertilizers, and in particular to a carbon source lactic acid fertilizer that promotes the growth of beneficial algae and its preparation method. Background Technology

[0002] Phytoplankton are an important biological group in the ecosystem of artificial aquaculture, playing a vital role in the material cycle and energy flow of the aquatic ecosystem. Imbalances between beneficial and harmful algae are common in artificial aquaculture, seriously affecting breeding efficiency and ecological stability. Traditional fertilizers often focus on single nutrient supplementation, such as providing only macronutrients like nitrogen, phosphorus, and potassium, making it difficult to simultaneously address the regulation of the microecological balance.

[0003] While traditional carbon-based fertilizers can promote algae growth, they lack targeted regulation of the microbial community, easily leading to the overgrowth of harmful algae. Although single probiotic preparations can decompose organic matter, they cannot synergistically provide the carbon and nitrogen nutrients required for algae growth. Furthermore, the accumulation of organic matter such as uneaten feed, excrement, and dead algae during aquaculture can easily cause water quality deterioration and increase disease risks. In addition, direct discharge of aquaculture wastewater into the ocean will have a very adverse impact on nearshore ecosystems. In conclusion, developing new formulations for improving aquaculture water quality is of great significance. Summary of the Invention

[0004] In view of this, the present invention proposes a carbon source lactic acid fertilizer that promotes the growth of beneficial algae and its preparation method, thereby solving the above problems.

[0005] The technical solution of this invention is implemented as follows: a carbon source lactic acid fertilizer that promotes the growth of beneficial algae, the carbon source lactic acid fertilizer comprising the following raw materials in parts by weight: 360-400 parts organic carbon source, 160-190 parts nitrogen source, 20-30 parts trace elements, 100-130 parts compound bacteria, 10-15 parts compound enzyme, and 30-45 parts complex; the compound bacteria are composed of lactic acid bacteria, yeast and Bacillus in a mass ratio of 50-60:20-30:35-40; the compound enzyme is composed of β-1,4-glucosidase, papain and β-amylase in a mass ratio of 8-12:3-5:1-2; the complex is composed of light calcium carbonate, diatomaceous earth and sepiolite powder in a mass ratio of 15-20:5-10:10-15.

[0006] Furthermore, the organic carbon source includes the following raw materials: molasses, corn starch and humic acid, in a mass ratio of 200-210:100-120:60-70.

[0007] Furthermore, the nitrogen source includes the following raw materials: amino acid powder, urea and yeast extract, with a mass ratio of 90-100:50-60:20-30.

[0008] Furthermore, the trace elements include the following raw materials in parts by weight: 5-8 parts chelated iron, 3-5 parts chelated manganese, 3-5 parts chelated zinc, 1-2 parts copper sulfate, 2-3 parts boric acid, 1-2 parts ammonium molybdate, and 5-8 parts magnesium sulfate.

[0009] Furthermore, the lactic acid bacteria consist of *Lactobacillus plantarum* and *Lactobacillus rhamnosus* in a mass ratio of 30-35:20-25.

[0010] Furthermore, the yeast is composed of Saccharomyces cerevisiae and Candida utilis in a mass ratio of 15-20:5-10.

[0011] Furthermore, the Bacillus is composed of Bacillus subtilis and Bacillus licheniformis in a mass ratio of 20-25:10-15.

[0012] Furthermore, the fineness of the light calcium carbonate is 7000-11000 mesh; the fineness of the diatomaceous earth is 200-300 mesh; and the fineness of the sepiolite is 80-100 mesh.

[0013] The present invention relates to a method for preparing a carbon source lactic acid fertilizer that promotes the growth of beneficial algae, comprising the following steps: (1) Mix the organic carbon source raw materials, add water at 50-60℃, stir evenly to make carbon source base material, and set aside; (2) Mix the nitrogen source raw material with trace elements to make nitrogen-trace element composite powder with a particle size of 80-100 mesh, and set aside for later use; (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 100-150 r / min for 5-10 min, gradually add the composite material, mix evenly, then add the composite enzyme, and continue stirring for 20-30 min; (4) Finally, add the compound bacterial product, stir at 300-400 r / min for 15-20 min, granulate, and obtain the target carbon source lactic acid fertilizer.

[0014] Further, in step (1), the solid-liquid ratio of the organic carbon source to water is 1:1.1-1.2.

[0015] Further, in step (4), granulation is performed to obtain particles with a diameter of 3-5 mm.

[0016] Compared with the prior art, the beneficial effects of the present invention are: (1) The carbon source lactic acid fertilizer of the present invention can precisely regulate the structure of algal communities, promote the growth of beneficial algae, and achieve multiple composite micro-ecological purification, synergistically enhance the stability of the ecosystem. Moreover, it is green, safe, efficient and long-lasting, and has a wide range of applications.

[0017] (2) The carbon source lactic acid fertilizer of the present invention can promote the growth of beneficial algae, improve the structure and composition of phytoplankton community in aquaculture water, optimize the physicochemical factors of aquaculture water, and improve the quality of aquaculture water more quickly.

[0018] (3) This invention provides high-quality nutrition for beneficial algae through the scientific ratio of organic carbon source, nitrogen source and trace elements, promoting their rapid reproduction and forming a dominant population; at the same time, it can regulate the pH value of the water body to a suitable range (6.5-7.5), effectively inhibiting the growth of harmful algae, fundamentally improving the algal community balance and enhancing the primary productivity of the water body.

[0019] (4) This invention, through the synergistic effect of compound bacteria, compound enzymes and compound complexes, can rapidly decompose organic matter such as dead algae residues, leftover feed and aquaculture excrement in water bodies, transforming them into small molecule nutrients that can be utilized by algae, removing harmful substances, reducing the risk of disease occurrence, achieving a benign ecological cycle, and significantly improving water transparency and dissolved oxygen levels.

[0020] (5) The carbon source lactic acid fertilizer of the present invention has three functions: promoting the growth of beneficial algae to fertilize the water, regulating the pH value to optimize the water environment, and synergistically removing dirt in the water to achieve a virtuous cycle of the water ecosystem. Detailed Implementation

[0021] Unless otherwise specified, the experimental methods used in the embodiments of this invention are conventional methods; Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0022] The bacterial strains used in the embodiments of this invention are all commercially available products.

[0023] A carbon source lactic acid fertilizer that promotes the growth of beneficial algae (I) Carbon source lactic acid fertilizer formula: 360-400kg organic carbon source, 160-190kg nitrogen source, 20-30kg trace elements, 100-130kg compound bacteria, 10-15kg compound enzymes, and 30-45kg compound compounds; 1. Organic carbon source: molasses 200-210kg, corn starch 100-120kg, humic acid 60-70kg; 2. Nitrogen source: 90-100 kg of amino acid powder (plant-based), 50-60 kg of urea (agricultural grade), and 20-30 kg of yeast extract powder; 3. Trace elements: 5-8 kg of chelated iron (EDTA-Fe), 3-5 kg ​​of chelated manganese (EDTA-Mn), 3-5 kg ​​of chelated zinc (EDTA-Zn), 1-2 kg of copper sulfate, 2-3 kg of boric acid, 1-2 kg of ammonium molybdate, and 5-8 kg of magnesium sulfate; 4. Compound probiotics: 50-60 kg of lactic acid bacteria preparation, 20-30 kg of yeast preparation, and 30-45 kg of Bacillus preparation; 4.1 Lactic acid bacteria preparations: Lactobacillus plantarum (≥10 10 CFU / g) 30-35kg, Lactobacillus rhamnosus (≥10) 10 CFU / g) 20-25kg; 4.2 Yeast preparations: Saccharomyces cerevisiae (≥10) 9 CFU / g) 15-20kg, Candida utilis (≥10) 9 CFU / g): 5-10 kg; 4.2 Bacillus preparations: Bacillus subtilis (≥10 10 CFU / g) 20-25kg, Bacillus licheniformis (≥10) 10 CFU / g): 10-15kg; 5. Complex enzymes: β-1,4-glucosidase (≥5000 U / g) 6-8 kg, papain (≥8000 U / g) 3-5 kg, β-amylase (≥10000 U / g) 1-2 kg; 6. Compound: 15-20 kg of light calcium carbonate (7000-11000 mesh), 5-10 kg of diatomaceous earth (200-300 mesh), and 10-15 kg of sepiolite powder (80-100 mesh).

[0024] (II) Preparation method of carbon source lactic acid fertilizer (1) Mix the organic carbon source raw materials (molasses, corn starch, humic acid), add tap water at 50-60℃ (solid-liquid ratio 1:1.2), stir evenly, and prepare carbon source base material for later use; (2) Mix the nitrogen source raw materials (amino acid powder, urea, yeast extract powder) and trace elements, and then pulverize them into nitrogen-trace element composite powder (particle size 80 mesh) for later use.

[0025] (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 100-150 r / min for 5-10 min, gradually add the composite material (light calcium carbonate, diatomaceous earth, sepiolite powder), mix evenly, and then add the composite enzyme (β-1,4-glucosidase, papain, β-amylase) at 25-30℃, and continue stirring for 20-30 min; (4) Finally, add compound bacteria (lactic acid bacteria preparation, yeast preparation, Bacillus preparation) at 30-35℃, stir at 300-400 r / min for 15-20 min, granulate (particles with a diameter of 3-5 mm) to obtain the target carbon source lactic acid fertilizer.

[0026] Example 1 (I) Carbon source lactic acid fertilizer formula: 380kg organic carbon source, 173kg nitrogen source, 25kg trace elements, 120kg compound bacteria, 12kg compound enzyme, and 40kg compound. 1. Organic carbon source: 205 kg molasses, 110 kg corn starch, 65 kg humic acid; 2. Nitrogen source: 95kg amino acid powder (plant source), 53kg urea (agricultural grade), 25kg yeast extract powder; 3. Trace elements: 6 kg of chelated iron (EDTA-Fe), 4 kg of chelated manganese (EDTA-Mn), 3.5 kg of chelated zinc (EDTA-Zn), 1.5 kg of copper sulfate, 2.5 kg of boric acid, 1.5 kg of ammonium molybdate, and 6 kg of magnesium sulfate; 4. Compound probiotics: 55kg of lactic acid bacteria preparation, 25kg of yeast preparation, and 40kg of Bacillus preparation; 4.1 Lactic acid bacteria preparations: Lactobacillus plantarum (≥10 10 CFU / g) 30kg, Lactobacillus rhamnosus (≥10) 10 25kg (CFU / g); 4.2 Yeast preparations: Saccharomyces cerevisiae (≥10) 9 15 kg of CFU / g, and ≥10 CFU / g of Candida utilis. 9 CFU / g): 10kg; 4.2 Bacillus preparations: Bacillus subtilis (≥10 10 CFU / g) 25kg, Bacillus licheniformis (≥10) 10 CFU / g): 15kg; 5. Complex enzymes: β-1,4-glucosidase (≥5000 U / g) 7kg, papain (≥8000 U / g) 4kg, β-amylase (≥10000 U / g) 1kg; 6. Compound: 18 kg of light calcium carbonate (8000 mesh), 8 kg of diatomaceous earth (200 mesh), and 14 kg of sepiolite powder (100 mesh).

[0027] (II) Preparation method of carbon source lactic acid fertilizer (1) Mix the organic carbon source raw materials (molasses, corn starch, humic acid), add 55℃ tap water (solid-liquid ratio 1:1.2), stir evenly, and prepare carbon source base material for later use; (2) Mix the nitrogen source raw materials (amino acid powder, urea, yeast extract powder) and trace elements, and then pulverize them into nitrogen-trace element composite powder (particle size 80 mesh) for later use.

[0028] (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 120 r / min for 8 min, gradually add the composite material (light calcium carbonate, diatomaceous earth, sepiolite powder), mix evenly, and then add the composite enzyme (β-1,4-glucosidase, papain, β-amylase) at 25-30℃, and continue stirring for 25 min. (4) Finally, add compound bacteria (lactic acid bacteria preparation, yeast preparation, and Bacillus preparation) at 30-35℃, stir at 350 r / min for 18 min, granulate (particles with a diameter of 3-5 mm) to obtain the target carbon source lactic acid fertilizer.

[0029] Example 2 (I) Carbon source lactic acid fertilizer formula: 360kg organic carbon source, 190kg nitrogen source, 30kg trace elements, 110kg compound bacteria, 15kg compound enzyme, and 45kg compound. 1. Organic carbon source: 200kg molasses, 100kg corn starch, 60kg humic acid; 2. Nitrogen source: 100kg amino acid powder (plant source), 60kg urea (agricultural grade), 30kg yeast extract powder; 3. Trace elements: 8 kg of chelated iron (EDTA-Fe), 5 kg of chelated manganese (EDTA-Mn), 5 kg of chelated zinc (EDTA-Zn), 2 kg of copper sulfate, 3 kg of boric acid, 2 kg of ammonium molybdate, and 5 kg of magnesium sulfate; 4. Compound probiotics: 50kg of lactic acid bacteria preparation, 23kg of yeast preparation, and 37kg of Bacillus preparation; 4.1 Lactic acid bacteria preparations: Lactobacillus plantarum (≥10 10 CFU / g) 30kg, Lactobacillus rhamnosus (≥10) 10 (CFU / g) 20kg; 4.2 Yeast preparations: Saccharomyces cerevisiae (≥10) 9 15 kg of CFU / g, and ≥10 CFU / g of Candida utilis. 9 (CFU / g) 8kg; 4.2 Bacillus preparations: Bacillus subtilis (≥10 10 CFU / g) 25kg, Bacillus licheniformis (≥10) 10 CFU / g): 12kg; 5. Complex enzymes: β-1,4-glucosidase (≥5000 U / g) 8kg, papain (≥8000 U / g) 5kg, β-amylase (≥10000 U / g) 2kg; 6. Compound: 20 kg of light calcium carbonate (8000 mesh), 10 kg of diatomaceous earth (200 mesh), and 15 kg of sepiolite powder (100 mesh).

[0030] (II) Preparation method of carbon source lactic acid fertilizer (1) Mix the organic carbon source raw materials (molasses, corn starch, humic acid), add 55℃ tap water (solid-liquid ratio 1:1.2), stir evenly, and prepare carbon source base material for later use; (2) Mix the nitrogen source raw materials (amino acid powder, urea, yeast extract powder) and trace elements, and then pulverize them into nitrogen-trace element composite powder (particle size 80 mesh) for later use.

[0031] (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 120 r / min for 8 min, gradually add the composite material (light calcium carbonate, diatomaceous earth, sepiolite powder), mix evenly, and then add the composite enzyme (β-1,4-glucosidase, papain, β-amylase) at 25-30℃, and continue stirring for 25 min. (4) Finally, add compound bacteria (lactic acid bacteria preparation, yeast preparation, and Bacillus preparation) at 30-35℃, stir at 350 r / min for 18 min, granulate (particles with a diameter of 3-5 mm) to obtain the target carbon source lactic acid fertilizer.

[0032] Example 3 (I) Carbon source lactic acid fertilizer formula: 400kg organic carbon source, 160kg nitrogen source, 30kg trace elements, 120kg compound bacteria, 10kg compound enzyme, and 30kg compound. 1. Organic carbon source: 210 kg molasses, 120 kg corn starch, 70 kg humic acid; 2. Nitrogen source: 90kg amino acid powder (plant source), 50kg urea (agricultural grade), 20kg yeast extract; 3. Trace elements: 5 kg of chelated iron (EDTA-Fe), 5 kg of chelated manganese (EDTA-Mn), 5 kg of chelated zinc (EDTA-Zn), 2 kg of copper sulfate, 3 kg of boric acid, 2 kg of ammonium molybdate, and 8 kg of magnesium sulfate; 4. Compound probiotics: 60kg of lactic acid bacteria preparation, 30kg of yeast preparation, and 30kg of Bacillus preparation; 4.1 Lactic acid bacteria preparations: Lactobacillus plantarum (≥10 10 CFU / g) 35kg, Lactobacillus rhamnosus (≥10) 10 25kg (CFU / g); 4.2 Yeast preparations: Saccharomyces cerevisiae (≥10) 9 20 kg of CFU / g, and ≥10 CFU / g of Candida utilis. 9 CFU / g): 10kg; 4.2 Bacillus preparations: Bacillus subtilis (≥10 10CFU / g) 20kg, Bacillus licheniformis (≥10) 10 CFU / g): 10kg; 5. Complex enzymes: β-1,4-glucosidase (≥5000 U / g) 6kg, papain (≥8000 U / g) 3kg, β-amylase (≥10000 U / g) 1kg; 6. Compound: 15 kg of light calcium carbonate (8000 mesh), 5 kg of diatomaceous earth (200 mesh), and 10 kg of sepiolite powder (100 mesh).

[0033] (II) Preparation method of carbon source lactic acid fertilizer (1) Mix the organic carbon source raw materials (molasses, corn starch, humic acid), add 55℃ tap water (solid-liquid ratio 1:1.2), stir evenly, and prepare carbon source base material for later use; (2) Mix the nitrogen source raw materials (amino acid powder, urea, yeast extract powder) and trace elements, and then pulverize them into nitrogen-trace element composite powder (particle size 80 mesh) for later use.

[0034] (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 120 r / min for 8 min, gradually add the composite material (light calcium carbonate, diatomaceous earth, sepiolite powder), mix evenly, and then add the composite enzyme (β-1,4-glucosidase, papain, β-amylase) at 25-30℃, and continue stirring for 25 min. (4) Finally, add compound bacteria (lactic acid bacteria preparation, yeast preparation, and Bacillus preparation) at 30-35℃, stir at 350 r / min for 18 min, granulate (particles with a diameter of 3-5 mm) to obtain the target carbon source lactic acid fertilizer.

[0035] Example 4 (a) The carbon source lactic acid fertilizer formula is the same as that in Example 1.

[0036] (II) Preparation method of carbon source lactic acid fertilizer (1) Mix the organic carbon source raw materials (molasses, corn starch, humic acid), add 50℃ tap water (solid-liquid ratio 1:1.2), stir evenly, and prepare carbon source base material for later use; (2) Mix the nitrogen source raw materials (amino acid powder, urea, yeast extract powder) and trace elements, and then pulverize them into nitrogen-trace element composite powder (particle size 80 mesh) for later use.

[0037] (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 100 r / min for 10 min, gradually add the composite material (light calcium carbonate, diatomaceous earth, sepiolite powder), mix evenly, and then add the composite enzyme (β-1,4-glucosidase, papain, β-amylase) at 25-30℃, and continue stirring for 20 min. (4) Finally, add compound bacteria (lactic acid bacteria preparation, yeast preparation, Bacillus preparation) at 30-35℃, stir at 300 r / min for 20 min, granulate (particles with a diameter of 3-5 mm) to obtain the target carbon source lactic acid fertilizer.

[0038] Example 5 (a) Carbon source lactic acid fertilizer formula The formula is the same as that in Example 1.

[0039] (II) Preparation method of carbon source lactic acid fertilizer (1) Mix the organic carbon source raw materials (molasses, corn starch, humic acid), add 60℃ tap water (solid-liquid ratio 1:1.2), stir evenly, and prepare carbon source base material for later use; (2) Mix the nitrogen source raw materials (amino acid powder, urea, yeast extract powder) and trace elements, and then pulverize them into nitrogen-trace element composite powder (particle size 80 mesh) for later use.

[0040] (3) Mix the carbon source material with the nitrogen-trace element composite powder, stir at low speed of 150 r / min for 5 min, gradually add the composite material (light calcium carbonate, diatomaceous earth, sepiolite powder), mix evenly, and then add the composite enzyme (β-1,4-glucosidase, papain, β-amylase) at 25-30℃, and continue stirring for 30 min. (4) Finally, add compound bacteria (lactic acid bacteria preparation, yeast preparation, Bacillus preparation) at 30-35℃, stir at 400 r / min for 15 min, granulate (particles with a diameter of 3-5 mm) to obtain the target carbon source lactic acid fertilizer.

[0041] Comparative Example 1 The difference from Example 1 is that the compound bacteria does not contain Bacillus subtilis, but the amounts of lactic acid bacteria and yeast are increased, while the total amount of compound bacteria remains unchanged. The compound bacteria consists of 75 kg of lactic acid bacteria preparation and 65 kg of yeast preparation. The lactic acid bacteria preparation includes: *Lactobacillus plantarum* (≥10...). 10 40 kg of CFU / g, and Lactobacillus rhamnosus (≥10 CFU / g) 10 35kg (CFU / g); Yeast preparation: Saccharomyces cerevisiae (≥10 CFU / g) 9 45 kg of CFU / g, and ≥10 CFU / g of Candida utilis. 9 CFU / g): 20kg.

[0042] Comparative Example 2 The difference from Example 1 is that the compound enzyme does not contain papain, but the amounts of β-1,4-glucosidase and β-amylase are increased, while the total amount of the compound enzyme remains unchanged. The compound enzyme consists of 10 kg of β-1,4-glucosidase (≥5000 U / g) and 2 kg of β-amylase (≥10000 U / g).

[0043] Comparative Example 3 The difference from Example 1 is that the composite does not contain light calcium carbonate, and the amount of diatomaceous earth and sepiolite powder is increased, while the total amount of the composite remains unchanged. The composite consists of 16 kg of diatomaceous earth (200 mesh) and 24 kg of sepiolite powder (100 mesh).

[0044] Comparative Example 4 The difference from Example 1 is that the lactic acid bacteria preparation does not contain Lactobacillus rhamnosus, and uses only Lactobacillus plantarum.

[0045] Comparative Example 5 The difference from Example 1 is that the yeast preparation does not contain Candida utilis, and all of it uses Saccharomyces cerevisiae.

[0046] Comparative Example 6 The difference from Example 1 is that the Bacillus preparation does not contain Bacillus licheniformis, and uses Bacillus subtilis exclusively.

[0047] Test case The water source for the experimental aquaculture was the high-level pond of the shrimp farming base in Chengmai County, Hainan Province. After the rainstorm, the water was introduced into each experimental pond. Shrimp of the same density and size were stocked in each experimental pond. The area of ​​each experimental pond was 1 mu. Carbon source lactic acid fertilizer from Examples 1-3 or Comparative Examples 1-6 was used after the rainstorm.

[0048] Application method: In a cool and ventilated place, mix the carbon source lactic acid fertilizer with an appropriate amount of water and then sprinkle it into the test pond. Use 0.4 kg of carbon source lactic acid fertilizer per acre.

[0049] Set up a control group: Sprinkle an equal amount of water.

[0050] Water quality in aquaculture water bodies was tested before and the day after use to compare changes in water quality; the diversity index was used to analyze these changes. H and uniformity index J A comprehensive evaluation of the water quality of aquaculture water bodies was conducted.

[0051] (1) Diversity Index H = - Σ( n i / N) log2(n i / N) In the formula, N: total number of phytoplankton cells; n i :i The number of cells in a species.

[0052] Diversity index rating levels: 0 to <1 (heavy pollution); 1 to 3 (medium pollution); >3 (light pollution or no pollution).

[0053] (2) Evenness index J = H / lnS In the formula, H is the diversity index, and S is the total number of phytoplankton species.

[0054] Uniformity index rating: 0 to <0.3 (heavy pollution); 0.3 to <0.5 (medium pollution); 0.5 to 0.8 (light pollution or no pollution).

[0055] The water quality of the aquaculture water body was tested before use. The average diversity index was 0.64 (heavily polluted), and the average evenness index was 0.25 (heavily polluted).

[0056] Table 1. Diversity index of aquaculture water after use

[0057] Table 2 Uniformity Index of Aquaculture Water After Use

[0058] The results showed that, compared with the control group, the aquaculture water quality was improved to some extent when using the carbon source lactic acid fertilizers in the examples or comparative examples. Specifically, the aquaculture water quality was significantly improved when using the carbon source lactic acid fertilizers of Examples 1-3 of this invention, all reducing the pollution level to slightly polluted or unpolluted. The carbon source lactic acid fertilizer of this invention can promote the growth of beneficial algae, improve the structure and composition of phytoplankton communities in the aquaculture water, optimize the physicochemical factors of the aquaculture water, and rapidly improve the aquaculture water quality.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention.

Claims

1. A carbon source lactic acid fertilizer for promoting the growth of beneficial algae, characterized by, The carbon source lactic acid fertilizer comprises the following raw materials in parts by weight: organic carbon source 360-400 parts, nitrogen source 160-190 parts, trace elements 20-30 parts, compound bacteria 100-130 parts, compound enzyme 10-15 parts, and compound 30-45 parts. The compound bacteria are composed of lactic acid bacteria, yeast bacteria and bacillus spores in a mass ratio of 50-60:20-30:35-40; the lactic acid bacteria are composed of lactobacillus plantarum and lactobacillus rhamnosus; the yeast bacteria are composed of saccharomyces cerevisiae and candida utilis; and the bacillus spores are composed of bacillus subtilis and bacillus licheniformis. The compound enzyme is composed of β-1,4-glucosidase, papain and β-amylase in a mass ratio of 6-8:3-5:1-2. The compound is composed of light calcium carbonate, diatomite and sepiolite powder in a mass ratio of 15-20:5-10:10-15.

2. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The organic carbon source comprises the following raw materials: molasses, corn starch and humic acid, and the mass ratio of the molasses, corn starch and humic acid is 200-210:100-120:60-70.

3. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The nitrogen source comprises the following raw materials: amino acid powder, urea and yeast extract powder, and the mass ratio of the amino acid powder, urea and yeast extract powder is 90-100:50-60:20-30.

4. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The trace elements comprise the following raw materials in parts by weight: chelated iron 5-8 parts, chelated manganese 3-5 parts, chelated zinc 3-5 parts, copper sulfate 1-2 parts, boric acid 2-3 parts, ammonium molybdate 1-2 parts, and magnesium sulfate 5-8 parts.

5. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The lactic acid bacteria are composed of lactobacillus plantarum and lactobacillus rhamnosus in a mass ratio of 30-35:20-25.

6. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The yeast bacteria are composed of saccharomyces cerevisiae and candida utilis in a mass ratio of 15-20:5-10.

7. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The bacillus spores are composed of bacillus subtilis and bacillus licheniformis in a mass ratio of 20-25:10-15.

8. The carbon source lactic acid fertilizer for promoting growth of beneficial algae according to claim 1, characterized by, The light calcium carbonate has a fineness of 7000-11000 mesh; the diatomite has a fineness of 200-300 mesh; and the sepiolite has a fineness of 80-100 mesh.

9. The method of producing a carbon source lactic acid fertilizer for promoting the growth of beneficial algae according to any one of claims 1 to 8, characterized by, The method comprises the following steps: (1) mixing the organic carbon source raw materials, adding water at 50-60°C, stirring uniformly to prepare a carbon source base, and reserving; (2) mixing the nitrogen source raw materials with the trace elements to prepare a nitrogen-trace element compound powder having a particle size of 80-100 mesh, and reserving; (3) mixing the carbon source base with the nitrogen-trace element compound powder, stirring at a low speed of 100-150 r / min for 5-10 min, gradually adding the compound, mixing uniformly, then adding the compound enzyme, and continuing to stir for 20-30 min; (4) finally adding the compound bacteria product, stirring at a high speed of 300-400 r / min for 15-20 min, and granulating to obtain the target carbon source lactic acid fertilizer.

10. The method for preparing the carbon source lactic acid fertilizer for promoting the growth of beneficial algae according to claim 9, characterized in that, In step (1), the solid-liquid ratio of the organic carbon source to water is 1:1.1-1.2; and in step (4), the granulation treatment obtains particles having a diameter of 3-5 mm.

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