Recombinant human collagen type iii and methods of making and using the same

By designing specific amino acid sequences and optimizing codon bias, recombinant human type III collagen was expressed in Pichia pastoris, which solved the problem of low activity of recombinant collagen and achieved efficient transdermal absorption and significant effects in promoting skin repair and moisturizing.

CN121342961BActive Publication Date: 2026-06-02SHANGHAI YUSONG BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI YUSONG BIOTECHNOLOGY CO LTD
Filing Date
2025-12-17
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

In the existing technology, there are problems such as low activity of recombinant collagen during the preparation process and ineffective or inhibitory effect on the expression of some beneficial target proteins.

Method used

By designing recombinant human type III collagen with a specific amino acid sequence, expressing it in Pichia pastoris using genetic engineering technology, optimizing codon bias to improve expression efficiency, and performing appropriate chemical and fluorescent modifications, the product's high hydrophilicity and humanized characteristics are ensured.

Benefits of technology

It improved the transdermal absorption efficiency of recombinant human type III collagen, reduced the risk of allergic reactions, and significantly regulated gene expression in fibroblasts and keratinocytes, promoting cell proliferation and migration, and enhancing skin repair and moisturizing capabilities.

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Abstract

The application relates to the technical field of genetic engineering, and discloses a recombinant human collagen type III and a preparation method and application thereof, the amino acid sequence of the recombinant human collagen type III comprises the amino acid sequence shown in SEQ ID NO. 1, the amino acid sequence of the recombinant human collagen type III provided by the application has the characteristics of high hydrophilicity and uniform charge distribution, and the characteristics greatly improve the transdermal absorption efficiency of the product, so that the product can penetrate into the bottom layer of the skin more deeply and has better efficacy. Meanwhile, the 100% humanized amino acid sequence design of the product ensures high affinity of the product with the human skin and maximally reduces the risk of allergic reaction.
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