New cordyceps sobolifera strain as well as culture method and application thereof

Abstract

The invention discloses a novel cordyceps sobolifera strain and a culture method and application thereof, and belongs to the field of microbial technology and food production. The culture method of the novel cordyceps sobolifera strain comprises the following steps: 1) inoculating the novel cordyceps sobolifera strain into a culture medium consisting of oat and buckwheat, wherein the inoculation amount is greater than or equal to 20%; and 2) firstly performing dark culture for 5-7 days, then performing light culture for 10-12 days, alternately performing blue light irradiation for 12-18 hours every day and darkness for 12-6 hours every day in the light culture period, and performing stage temperature difference change culture in the light culture period, the light culture temperature is higher than the dark culture temperature, and the difference value is greater than or equal to 4 DEG C. According to the novel cordyceps sobolifera strain and the culture method and application thereof, the content of functional components such as polysaccharide and N6-(2-ethoxyl) adenosine is increased while beauveria bassiana is reduced, unification of safety and nutrition is achieved, and a solid technical support is provided for application of cordyceps sobolifera as a food or functional food raw material.

Description

Technical Field

[0001] This application belongs to the field of edible and medicinal fungi production technology, specifically a new strain of Cordyceps militaris, its cultivation method, and its uses. Background Technology

[0002] Cicada Flower ( Cordyceps cicadae ), formerly known as Cicada spores ( Isaria cicadae ), Penicillium cicadae ( Paecilomyces cicadae Cordyceps sinensis ( ), Cordyceps militaris ( Cordyceps chanhua ) Parasitic cicadas (a type of ascomycete, belonging to the Clavicipitaceae family) Cicadae flammata The fungus-insect complex formed after the nymphs of insects such as *Cicada nymph* are born is mainly distributed in East and Southwest my country. *Cicada nymph* has important medicinal value, especially its fruiting body, which has various effects such as improving sleep, enhancing immunity, strengthening the kidneys, fighting tumors, and lowering blood pressure, blood sugar, and cholesterol. Currently, *Cicada nymph* is widely cultivated artificially.

[0003] In 2020, the National Health Commission of China issued Announcement No. 9 of 2020, approving for the first time that artificially cultivated Cordyceps sinensis fruiting bodies can be used as a new food raw material, with broad development prospects. The announcement specifically mentioned the requirement that the beauvericin (BEA) content be ≤3mg / kg. Currently, there are no known technologies for cultivating Cordyceps sinensis to reduce BEA content. Therefore, there is a need to provide a new Cordyceps sinensis strain and its cultivation method to achieve a beauvericin content ≤3mg / kg, and to increase the content of active ingredients such as polysaccharides and N6-(2-hydroxyethyl) adenosine (HEA). Summary of the Invention

[0004] To address the shortcomings of existing technologies, this application aims to provide a technical solution for a new strain of Cordyceps militaris, its cultivation method, and its uses. This solution reduces the content of beauveria bassiana while increasing the content of polysaccharides and N6-(2-hydroxyethyl)adenosine, achieving a balance between safety and nutrition, and providing solid technical support for the application of Cordyceps militaris as a food ingredient.

[0005] To achieve the above objectives, this application adopts the following technical solution: This application provides a novel strain of Cordyceps militaris, which was deposited on December 29, 2016, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO: 13572.

[0006] This application also provides a method for culturing a new strain of Cordyceps militaris, comprising the following steps: 1) Inoculate the new strain of Cordyceps militaris into a culture medium consisting of 75-85 parts by weight of oats and 15-25 parts by weight of buckwheat, with an inoculation amount ≥20%; 2) first dark culture for 5-7 days, dark culture temperature is 16-20 DEG C; then light culture for 10-12 days, during light culture, 12-18 hours of blue light irradiation and 12-6 hours of darkness are alternately carried out every day; during light culture, temperature is 20-24 DEG C, light culture temperature is greater than dark culture temperature, and the difference is greater than or equal to 4 DEG C, the wavelength of blue light is 450-495 nm, the blue light irradiation intensity is 120-300 Lux; the relative humidity of dark culture is 75%-85%, the relative humidity of light culture is 80%-100%; the carbon dioxide concentration is 501-1100 PPM in the first 1 to 7 days of the light culture process, and the carbon dioxide concentration is 1201-3000 PPM in the 8th to 12th days of the light culture process.

[0007] The culture method of the new strain of Cordyceps cicadae, in step 1): the culture medium is composed of 80-82 parts of oat and 20-18 parts of buckwheat.

[0008] The culture method of the new strain of Cordyceps cicadae, in step 1): the thickness of the culture medium is 1.5-2.5 cm, preferably 2-2.2 cm, and the inoculation amount is 20-30%.

[0009] The culture method of the new strain of Cordyceps cicadae, in step 2): the wavelength of blue light is 465-480 nm, and the blue light irradiation intensity is 180-240 Lux.

[0010] The application of the new strain of Cordyceps cicadae in preparing food or functional food raw materials.

[0011] The present application adopts an inoculation amount higher than that of the traditional culture method, can quickly realize mycelium fullness, and the fermentation time of full mycelium is 1-2 days earlier than that of the traditional method. The thickness of the culture medium is 1.5 to 2.5 cm, which is thinner than that of the traditional culture medium, and the culture medium nutrients are ensured, and the mycelium is quickly full.

[0012] Compared with the prior art, the present application has the following beneficial effects: 1. The present application first applies the Cordyceps cicadae strain to the field of new food raw materials, and ensures that it meets the national safety standards for new food raw materials. In 2020, the National Health Commission announced (No. 9 in 2020) that artificially cultivated Cordyceps cicadae fruiting bodies can be used as new food raw materials, but it is clearly required that the deoxynucleoside content is less than or equal to 3 mg / kg. The deoxynucleoside content in the new strain of Cordyceps cicadae obtained by the culture method of the present application is far below this limit, which greatly improves the safety of the new strain as a new food raw material.

[0013] 2, in the prior art, cicada flower is mainly used as medicine, and there are few reports on the application of cicada flower and its extract in food. Most studies focus on the medicinal value of cicada flower, such as "anti-tumor, anti-inflammatory analgesic, improving kidney function, reducing blood sugar, reducing blood pressure and various pharmacological effects". The present application breaks through the traditional application range, successfully applies the new strain of cicada flower to the field of new food raw materials, expands its application range, and can be used for developing health food, functional food or ordinary food raw materials, and has important industrialization and commercialization value.

[0014] 3, the present application also focuses on the retention and improvement of nutritional components, reduces the debackin content while increases the content of polysaccharide and N6-(2-hydroxyethyl) adenosine, realizes the unity of safety and nutrition, and realizes the unity of safety and nutrition. This comprehensive quality improvement strategy has more industrial value than single safety control, and provides a solid technical support for the application of cicada flower as a food raw material. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 The growth of the fruiting body under different light culture.

[0016] Figure 2 The BEA content of the fruiting body under different light culture of the present application.

[0017] Figure 3 The BEA content of the fruiting body under different light culture of the present application.

[0018] Figure 4 The polysaccharide content of the fruiting body under different light culture.

[0019] Figure 5 The HEA content of the fruiting body under different light culture. DETAILED DESCRIPTION

[0020] In order to make the people in the art better understand the scheme of the present application, the technical scheme in the specific embodiment of the present application will be described clearly and completely in combination with the drawings in the embodiment of the present application.

[0021] A new strain of cicada flower is isolated from the Plant Protection and Entomophaga Fungus Research Room of Zhejiang Subtropical Crop Research Institute, and preserved in China General Microbiological Culture Collection Center on December 29, 2016, with the preservation number of CGMCC NO: 13572.

[0022] Example 1 1) inoculate the new strain of cicada flower in the culture medium composed of 75 parts by weight of oat and 25 parts of buckwheat, and the inoculation amount is 20%; 2) first dark culture for 6 days, dark culture temperature 20℃, dark culture relative humidity 85%; then light culture for 10 days at 24℃, light culture relative humidity 100%, during the light culture, blue light irradiation is adopted for 12 hours and darkness for 12 hours alternately every day, the light culture temperature is greater than the dark culture temperature, the difference is ≥4℃, the wavelength of the blue light is 450nm, and the blue light irradiation intensity is 300Lux; During the light culture, the carbon dioxide concentration is 1100PPM for the first 7 days, and the carbon dioxide concentration is 3000PPM for the 8th to 10th days of the light culture process, which can uniformly regulate the growth nodes of mycelium and fruiting body, and provide convenience for subsequent uniform harvesting of fruiting body.

[0023] Example 2 1) inoculate the new strain of cicada fungus into a culture medium composed of 80 parts by weight of oat and 20 parts of buckwheat, and the inoculation amount is 25%; 2) first dark culture for 7 days, dark culture temperature 18℃, dark culture relative humidity 80%; then light culture for 12 days at 22℃, light culture relative humidity 90%, during the light culture, blue light irradiation is adopted for 15 hours and darkness for 9 hours alternately every day, the light culture temperature is greater than the dark culture temperature, the difference is ≥4℃, the wavelength of the blue light is 470nm, and the blue light irradiation intensity is 260Lux; During the light culture, the carbon dioxide concentration is 700PPM for the first 7 days, and the carbon dioxide concentration is 1500PPM for the 8th to 12th days of the light culture process.

[0024] Example 3 1) inoculate the new strain of cicada fungus into a culture medium composed of 85 parts by weight of oat and 15 parts of buckwheat, and the inoculation amount is 30%; 2) first dark culture for 5 days, dark culture temperature 21℃, dark culture relative humidity 75%; then light culture for 10 days at 24℃, light culture relative humidity 80%, during the light culture, blue light irradiation is adopted for 18 hours and darkness for 6 hours alternately every day, the light culture temperature is greater than the dark culture temperature, the difference is ≥4℃, the wavelength of the blue light is 495nm, and the blue light irradiation intensity is 180Lux; During the light culture, the carbon dioxide concentration is 501PPM for the first 7 days, and the carbon dioxide concentration is 1201 PPM for the 8th to 10th days of the light culture process.

[0025] The beneficial effects of the present application are further demonstrated by the following corresponding experiments.

[0026] The main instruments for the culture and result analysis of the new strain of cicada fungus include: CN-LQC50001 electronic balance, Shanghai Yaoshen Electronic Technology Co., Ltd.; MLS-3750 sterilization pot, Sanyo Company, Japan; VS-1300L-U clean bench, Suzhou Antai Air Technology Co., Ltd., Sujing Group; SPH-2102 constant temperature incubation oscillator, Shanghai Shipei Co., Ltd.; GLF5-DL type-2 edible fungus liquid fungus multifunctional seed quantitative inoculator, Beigongwang Equipment Co., Ltd.; CT hot-C-0 type stainless steel hot air circulation oven, China Fanguan Drying Equipment Co., Ltd.; YXQ.WF22-0, 8SZ-00 type horizontal rectangular pressure steam sterilizer, Hunan Gongchuang Medical Equipment Co., Ltd.; QE-300 type high-speed pulverizer, Zhejiang Yilide Trade Co., Ltd.

[0027] QTRAPTM 5500 high performance liquid chromatography-tandem mass spectrometer (equipped with Exion LC liquid phase system and QTRAPTM 5500 mass spectrometer, model HPLC-MS / MS, manufacturer ABSciex, USA), solid phase extraction (SPE) column (200 mg, 3 mL, manufacturer Waters, USA), biosafety cabinet (manufacturer Beijing Dongfangzhesheng Co., Ltd.), biochemical incubator, analytical balance, optical microscope, and beat-type homogenizer (manufacturer Interscience, France).

[0028] RQ22-480J ultrasonic cleaner (manufacturer Shanghai Rongwei Instrument Co., Ltd.); DKB-10 refrigeration circulating water bath (manufacturer Changzhou Huapuda Teaching Instrument Co., Ltd.); ZNG50-1000 vacuum decompression concentrator (manufacturer Wenzhou Yunchi Technology Co., Ltd.); e2695 high performance liquid chromatograph (manufacturer Waters Co., Ltd., USA); LC-20AR semi-preparative high performance liquid chromatograph (manufacturer Shimadzu Co., Ltd., Japan).

[0029] The main reagents and materials for culturing and result analysis of the new strain of Cordyceps cicadae include: BEA standard (CAS: 26048-05-5), methanol, acetonitrile, ammonium acetate, all chromatographically pure, ultrapure water (prepared by a laboratory ultrapure water instrument). Methanol (chromatographically pure, manufacturer Anhui Tiandi High-purity Solvent Co., Ltd.); N6-(2-hydroxyethyl)-adenosine standard, cordycepin standard, ammonium acetate, formic acid (analytically pure, manufacturer Beijing Solabio Technology Co., Ltd.). Oats, commercially available; wheat, commercially available; rice, commercially available; sucrose, commercially available; potato, commercially available.

[0030] In the preliminary culture experiment, the culture medium was a liquid strain culture medium, which included 20% potato 200 g, 2% sucrose 20 g, and distilled water 1000 mL.

[0031] The experimental method includes the following steps: 1.2.1 Inoculation of spore suspension preparation The mycelium or conidia of the slant culture was inoculated into a sterilized conical flask in a sterile environment, and cultured in a constant temperature incubator at 120 r / min and 25℃ for 4 days.

[0032] 1.2.2 Effect of different light on Cordyceps cicadae Steamed oats, 1250g of oats were weighed into a culture pot, the oats were mixed evenly with a gloved hand, covered with plastic film, placed in a rectangular pressure steam sterilizer at 121℃, sterilized for 30min, and OW7 / 3 and OR7 / 3 media were prepared in the same way. The mycelium cultured in a shaking incubator was poured into a sterilized 1000mL flask in a sterile environment to prepare 3000mL. The input end of the multifunctional quantitative inoculator was connected to the prepared spore suspension, and the inoculation needle was inserted into the center of the plastic film to quantitatively spray. Send to the dark culture room for culture, not more than 10d, maintain 24℃, humidity 85%. Set up white light, red light, yellow light, green light and blue light five groups of different light, after the mycelium spreads, transfer to the culture room with different light for cultivation, and collect the fruiting bodies and dry them.

[0033] 1.2.3 Effect of different light intensity on Cordyceps cicadae According to the best light wavelength screened out in 2.2.2, the same culture conditions were used in the early stage, and the light intensity of 60, 120, 180, 240 and 300 lux was set when the light was transferred for culture. The fruiting bodies were harvested when they were mature and ready for use.

[0034] 1.2.4 Determination of dry weight of fruiting bodies The collected fruiting bodies were weighed, dried in an oven at 60℃, and then placed in a plastic bag. The fruiting bodies were crushed in a pulverizer to pass through a 200 mesh screen, and 5g of the sieved powder was collected in a plastic bag. The bag was sealed and numbered for detection.

[0035] 1.2.5 Beauvericin content determination The method of the National Health Commission Announcement (2020 No. 9) was used for detection.

[0036] 1.2.6 Determination of polysaccharide content According to the industry standard "NY / T 1676-2023 Determination of Crude Polysaccharide in Edible Fungi by Spectrophotometry". After the sample was extracted with hot water and precipitated with alcohol, the absorbance was determined by spectrophotometer, and the polysaccharide content was calculated with glucose as standard.

[0037] 1.2.7 N 6 -(2-hydroxyethyl) adenosine content determination Reference: Ai Renli, Tan Aijuan, Lv Shiming. N 6Optimization of extraction process and structural identification of (2-hydroxyethyl) adenosine [N6-(2-hydroxyethyl) adenosine, HEA]. Journal of Edible Fungi, 2023, 30(02): 65-74.

[0038] Results and analysis are as follows: 2.1 The effect of inoculum size on the growth of Cordyceps cicadae mycelium in automated production Under the same other culture conditions, increasing the inoculum size is conducive to the growth of mycelium and reduces the contamination rate. As shown in Table 1, the 20% inoculum size fills the mycelium 1 day earlier than the 10% inoculum size and 2 days earlier than the 5% inoculum size, and the contamination rate is also significantly reduced, with the 20% inoculum size having a contamination rate of 0. There is no significant difference between the 30% inoculum size and the 20% inoculum size. Using an inoculum size of 20% or more not only shortens the mycelium culture time but also increases the yield from the perspective of reducing the contamination rate. Since it is liquid inoculation, an inoculum size of 35% is too much water, which is not conducive to solid fermentation, and it takes 8 days to fill the mycelium, so the preferred inoculum size is 20-30%.

[0039] Table 1 The effect of inoculum size on the growth of Cordyceps cicadae mycelium Note: 10 pots per treatment, repeated 3 times, P=0.01 (indicates that if the original hypothesis is true, the probability of observing such or greater differences in Table 1 is only 1%).

[0040] 2.2 The effect of medium formula on the growth of Cordyceps cicadae fruiting bodies in automated production Under the same other culture conditions, adjusting the medium formula is conducive to inhibiting the production of BEA. Buckwheat is beneficial to the increase of fruiting body yield but is not conducive to the inhibition of BEA production. Considering various factors, the best choice is oat:buckwheat=4:1, which has high yield and low BEA content, as shown in Table 2.

[0041] Table 2 The effect of medium formula on the growth of Cordyceps cicadae fruiting bodies Note: Each treatment is repeated 3 times, P=0.01.

[0042] Both oatmeal and buckwheat are common grains on the market, which are low in price and cost, and are conducive to industrialized production. The specific ratio of oatmeal and buckwheat produces a "1+1>2" effect, which increases the yield while controlling the BEA content. In addition, it provides the optimal nutrient ratio required for mycelial growth and fruiting body development, promoting the accumulation of active ingredients.

[0043] 2.3 The effect of culture temperature and temperature difference on the growth of Cordyceps cicadae fruiting bodies in automated production Under otherwise identical culture conditions, a lower culture temperature is beneficial for inhibiting the production of BEA in Cordyceps sinensis fruiting bodies, and a greater temperature difference between dark and light culture is beneficial for reducing the BEA content of fruiting bodies and increasing the yield of fruiting bodies, as shown in Table 3.

[0044] Table 3. Effects of temperature on the growth of Cordyceps militaris Note: Each treatment consisted of 10 pots, repeated 3 times, P=0.01.

[0045] This application employs a combination of "dark culture temperature of 16 to 20°C" and "light culture temperature of 20 to 24°C" to ensure a temperature difference of 2-4°C. Experiments show that the combination of 16°C dark culture and 20°C light culture yields the lowest BEA content and a higher fruiting body yield.

[0046] 2.4 Effects of different light levels on the yield and BEA content of Cordyceps militaris fruiting bodies in automated production Under identical culture conditions, no fruiting body differentiation was observed under red and yellow light (e.g.) Figure 1 As shown), the fruiting bodies obtained by culturing under other different lighting conditions (white light, blue light, and green light) (such as...) Figure 1 (As shown in the figure) The samples were dried, weighed, and tested for beauvericin (BEA). The results are shown in Table 4 and Figure 2 As shown, the dry weight of fruiting bodies under blue light is significantly higher than that under green light and white light, while the content of beauveria bassiana is significantly lower than that under green light and white light. The dry weight of fruiting bodies under green light is the lowest, while the content of beauveria bassiana is the highest. The dry weight of fruiting bodies under white light is moderate, and the content of beauveria bassiana is moderate. Therefore, blue light is preferred.

[0047] Table 4. Dry weight and BEA content of fruiting bodies cultured under different light conditions. 2.5 Effects of different blue light intensities on automated production of Cordyceps militaris The results under different blue light irradiation conditions are shown in Table 5 and... Figure 3 As shown, the highest Beauveria bassiana content was observed under 60 lux light intensity; the content was slightly lower under 120 lux light intensity, already below the limit of 3 mg / kg; the content was even lower at 180 lux, at 1.574 ± 0.3 mg / kg, far below the limit of 3 mg / kg; the lowest content was observed under 240 lux light intensity, at 0.316 ± 0.137 mg / kg. There was no significant difference in Beauveria bassiana content between 300 lux and 240 lux. Therefore, 180 lux-300 lux is a suitable blue light intensity, and 180-240 lux is preferred to ensure the lowest BEA content while saving on electricity costs.

[0048] Table 5 BEA content (mg / kg) of different light intensity blue light cultured fruiting bodies 60 lux 120 lux 180 lux 240 lux 300 lux 3.95 2.47 1.59 0.286 0.42 3.64 2.64 1.729 0.34 0.355 4.037 2.933 1.522 0.45 0.249 3.184 2.43 1.584 0.471 0.549 7.377 2.954 1.964 0.244 0.614 3.99 2.478 1.053 0.105 0.509 4.363±1.511a 2.651±0.238b 1.574±0.300c 0.316±0.137d 0.449±0.135d 2.6 Blue light is conducive to accumulation of effective components in Cordyceps cicadae fruiting bodies Through the experiment, the polysaccharide and N6-(2-hydroxyethyl) adenosine contents of fruiting bodies cultured under different light were detected. It was found that under blue light and white light, the polysaccharide content of fruiting bodies had no significant difference, but was significantly higher than that under green light. Under blue light, the N6-(2-hydroxyethyl) adenosine content of fruiting bodies was significantly higher than that under green light and white light. Polysaccharide and N6-(2-hydroxyethyl) adenosine are the main effective components of Cordyceps cicadae, and the accumulation of both is the most under blue light. Therefore, blue light is preferably selected as the light source, and the results are shown in Tables 6 and Figure 4 , 7 and Figure 5 .

[0049] Table 6 Polysaccharide content (mg / g) of fruiting bodies cultured under different light Green light Blue light White light 52.1 60.7 61.9 41.3 62.6 60.4 44.2 58.2 61.6 45.87±5.59b 60.5±2.21a 61.3±0.79a Table 7 N6-(2-hydroxyethyl) adenosine (HEA) content (mg / 100g) of fruiting bodies cultured under different light Green light Blue light White light 65.2 90.1 78.5 66.8 89.6 77.9 67.3 91.7 79.7 66.4±1.2a 90.5±1.2c 78.7±0.84b The application also provides an application of the new Cordyceps cicadae strain in preparing food or functional food raw materials. The food is, for example, powder, capsule, beverage, etc.

[0050] It should be noted that the "first", "second" and similar words used in the specification and claims of the present application do not represent any order, quantity or importance, but are only used to distinguish different components. Similarly, "one" or "a" and similar words do not represent a quantity limitation, but represent the existence of at least one. "Multiple" or "several" means at least two. Unless otherwise indicated, "before", "after", "left", "right", "below" and / or "above" and similar words are only for ease of description, and are not limited to a position or a spatial orientation. "Include" or "contain" and similar words mean that the elements or objects appearing before "include" or "contain" cover the elements or objects listed after "include" or "contain" and their equivalents, and do not exclude other elements or objects. "Connected" or "connected" and similar words are not limited to physical or mechanical connection, but can include electrical connection, whether direct or indirect.

[0051] As used in the specification and the appended claims, the singular forms "a," "an" and "the" are intended to include plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and / or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof.

[0052] It is to be understood that the above description and the accompanying examples are illustrative of the application and are not intended to limit the scope of the present application. Numerous variations and modifications will become apparent to those skilled in the art once the above description is available. It is intended that all such variations and modifications be included within the scope of the application as described in the appended claims.

Claims

1. A new strain of Cordyceps, which was preserved in China General Microbiological Culture Collection Center on December 29, 2016, and the preservation number is CGMCC NO: 13572.

2. The method for culturing a new strain of *Cicada nymph* as described in claim 1, characterized in that... The method comprises the following steps: 1) inoculating the new strain of Cordyceps into a culture medium composed of 75-85 parts by weight of oat and 15-25 parts of buckwheat, and the inoculation amount is ≥20%; 2) first dark culture for 5-7 days at a temperature of 16-20℃, and then light culture for 10-12 days, during which 12-18 hours of blue light irradiation and 12-6 hours of darkness are alternately performed every day; during the light culture, the temperature is 20-24℃, the light culture temperature is higher than the dark culture temperature, and the difference is ≥4℃, the wavelength of the blue light is 450-495nm, the blue light irradiation intensity is 120-300Lux, the relative humidity during the dark culture is 75%-85%, and the relative humidity during the light culture is 80%-100%; The carbon dioxide concentration is 501-1100PPM during 1 to 7 days before the light culture process, and the carbon dioxide concentration is 1201-3000PPM during 8 to 12 days of the light culture process.

3. The culture method of the new strain of C. formosana according to claim 2, characterized in that In step 1), the culture medium is composed of 80-82 parts of oat and 20-18 parts of buckwheat.

4. The culture method of a new strain of C. formosana according to claim 2, characterized in that In step 1), the thickness of the culture medium is 1.5-2.5cm, preferably 2-2.2cm, and the inoculation amount is 20-30%.

5. The culture method of a new strain of C. formosana according to claim 2, wherein the culture medium is a liquid medium. In step 2), the wavelength of the blue light is 465-480nm, and the blue light irradiation intensity is 180-240Lux. 6.The application of the new strain of Cordyceps in claim 1 in the preparation of food or functional food raw materials.

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