Aptamer-antibody dual recognition coupled three-dimensional inverse opal hydrogel photonic crystal fluorescence enhancement multi-marker detection method

By combining a nucleic acid aptamer-antibody dual recognition system with three-dimensional inverse opal hydrogel photonic crystal fluorescence enhancement technology, the sensitivity and specificity issues of multi-target detection in breast cancer have been resolved, achieving high-sensitivity and high-specificity multi-target detection, which is suitable for early diagnosis and personalized treatment of breast cancer.

CN121347465BActive Publication Date: 2026-07-07JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Filing Date
2025-09-08
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

Existing technologies for single biomarker detection in breast cancer diagnosis suffer from insufficient sensitivity and specificity, making it difficult to achieve highly sensitive and specific multi-target combined detection, leading to false positive or false negative results and affecting early diagnosis and personalized treatment.

Method used

A fluorescence enhancement strategy using a nucleic acid aptamer-antibody dual recognition system coupled with a three-dimensional inverse opal hydrogel photonic crystal was adopted. By designing three-dimensional inverse opal hydrogel photonic crystals with different reflected light colors as carriers, and combining aptamers and antibodies that specifically recognize targets, a cyclic chain hybridization reaction system was established to achieve signal amplification and simultaneous optical detection of multiple targets.

Benefits of technology

It achieves high sensitivity and high specificity in multi-target detection, and can simultaneously detect breast cancer-related CEA, CA15-3 and HER2, significantly improving detection accuracy and making it suitable for early diagnosis and personalized treatment guidance.

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Abstract

The application relates to a multi-marker detection method of aptamer-antibody dual recognition coupling three-dimensional inverse opal hydrogel photonic crystal fluorescence enhancement, and belongs to the field of analytical chemistry and biomedical technology. Specifically, first, SiO2 nanoparticles with different particle sizes are synthesized to prepare a photonic crystal PhCs template, hydrogel precursors are permeated into the PhCs gap to obtain three-dimensional IOPCHs with different structural colors, then different target antibodies are modified to the surfaces of the different IOPCHs to realize specific recognition and enrichment of multiple targets. The detection part adopts a pre-constructed Aptamer-HCR compound, when the target is captured by the antibody fixed on the IOPCH surface, the corresponding compound directly combines the target through the aptamer, so that the amplified fluorescence signal is anchored on the IOPCH with a specific color, the PBG effect of the IOPCHs can inhibit non-radiative fluorescence decay, and the signal intensity is significantly enhanced, and the detection sensitivity is improved.
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