Preparation method and application of Chinese torreya hydrolat

Abstract

The invention discloses a preparation method of torreya grandis hydrolat and application of the torreya grandis hydrolat in acne improvement, and belongs to the field of plant extracts. The Chinese torreya hydrolat is obtained by performing steam distillation, condensation and oil-water separation on Chinese torreya aril to obtain a water phase and then performing filtration. The scavenging rates of DPPH and ABTS < + > free radicals are 85% and 90% or above respectively, and good oxidation resistance is achieved. Besides, antibacterial tests show that the Chinese torreya hydrolat can remarkably inhibit proliferation of propionibacterium acnes, has little influence on staphylococcus epidermidis and can be applied to cosmetics for improving acnes.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of plant extracts, and relates to a preparation method of torreya grandis hydrolat and application thereof. BACKGROUND

[0002] Acne is a chronic inflammatory common skin disease, which is mostly seen in adolescents, and the incidence rate is as high as more than 90%. Although the disease generally does not endanger life, it can cause patients' appearance or disfigurement due to its high incidence on the face, and has a considerable psychological impact on patients, and can even cause psychological diseases such as anxiety and depression, so it cannot be ignored. The occurrence of acne is closely related to factors such as excessive sebum secretion, keratinization of hair follicle sebaceous duct, pathogenic bacterial infection and inflammation. Excessive secretion of sebum from sebaceous glands causes pore blockage, and a large number of Propionibacterium acnes which can cause inflammation in the hair follicle proliferate. Studies have shown that there is skin flora imbalance in the skin of acne patients, the number of Propionibacterium acnes increases, and the number of Staphylococcus epidermidis decreases, the flora ratio is unbalanced, leading to a large number of Propionibacterium acnes proliferation, secreting lipase to hydrolyze triglyceride, and metabolizing more free fatty acids, thereby aggravating inflammatory reaction.

[0003] At present, acne is mainly treated by using hormone antagonists, retinoid drugs, antibiotics and other drugs, but long-term use of drugs has the risk of causing allergies, kidney function damage, and even causing deformity and other toxic side effects. Selecting acne-removing cosmetic raw materials with the functions of restoring flora balance and anti-inflammatory ingredients is a safe and effective means for preventing and treating acne.

[0004] Torreya grandis belongs to Taxaceae and is a evergreen tree, which is a special product in China and a rare economic tree species in the world. Torreya grandis seed is the kernel of Torreya grandis, which has the medicinal values of killing insects, removing stagnation, moistening lung and reducing phlegm, lubricating intestines and eliminating, invigorating spleen and tonifying qi, removing blood stasis and generating new ones. Torreya grandis aril is a layer of wrapping material outside the Torreya grandis seed, and the weight ratio of Torreya grandis aril to seed is about 1:1. Although Torreya grandis aril is produced in large quantities in the processing of Torreya grandis seed, it is often treated as waste, leading to problems of resource waste and environmental pollution. Torreya grandis aril has the characteristics of citrus aroma and is rich in various active secondary metabolites such as essential oil, diterpenoid compounds and flavonoids. Therefore, Torreya grandis aril has broad application prospects in the fields of food, medicine, perfume essence and cosmetics. SUMMARY

[0005] The purpose of the present application is to provide a Torreya grandis hydrolat prepared from Torreya grandis aril, which has good antioxidant effect and can regulate skin flora balance and is used for preventing and / or treating acne.

[0006] The specific technical scheme is as follows:

[0007] The application of a Torreya grandis hydrolat in preparing the following products,

[0008] 1) products for preventing and / or treating acne;

[0009] 2) products with antioxidant effect;

[0010] 3) products with anti-inflammatory effect;

[0011] 4) products with DPPH free radical and ABTS+free radical scavenging capacity;

[0012] 5) products for regulating skin flora balance;

[0013] 6) products for inhibiting growth of Propionibacterium acnes.

[0014] The Torreya grandis pure distillate is obtained by water vapor distillation, condensation, oil-water separation to obtain an aqueous phase, and then filtration of the Torreya grandis aril.

[0015] Preferably, the Torreya grandis aril is fresh aril.

[0016] In some embodiments, the weight-volume ratio of the Torreya grandis aril to water in the water vapor distillation is 1:2-5 (Kg:L).

[0017] In some embodiments, the distillation temperature in the water vapor distillation is 80-100°C, and the distillation time is 1-5h; preferably, the distillation temperature is 90-95°C, and the distillation time is 2.5-3h.

[0018] In some embodiments, the filtration is membrane filtration, preferably using a 0.3-0.7μm filter membrane, and further preferably a 0.45μm filter membrane.

[0019] In some embodiments, the concentration of the Torreya grandis pure distillate is 20-100mg / mL, preferably 1.562-25mg / mL, and further preferably 25mg / mL.

[0020] In some embodiments, the product is a topical product or an internal product, the topical product is selected from skin care products, cosmetics, hair care products; and the internal product is selected from solid beverages, liquid beverages, dairy products, seasoning powders, nutritional products, and internal medical health care products.

[0021] In some embodiments, the topical product is selected from emulsions, gels, creams, microneedle patches, and masks.

[0022] In some embodiments, the external use product further contains an auxiliary material containing a preservative ingredient: one or more selected from the group consisting of methylparaben, ethylparaben, propylparaben, formaldehyde releasers, methylchloroisothiazolinone, methylisothiazolinone, phenoxyethanol, imidazolidinyl urea, isothiazolinone, phenol, benzoic acid, benzyl alcohol, potassium sorbate, bronopol, p-hydroxyacetophenone, chlorphenesin, triclosan, quaternary ammonium salt-15, dehydroacetic acid and salts thereof.

[0023] In some embodiments, the external use product further contains an auxiliary material containing an emulsifying ingredient: one or more selected from the group consisting of polyethylene glycols, glycerol esters, sugar derivatives, anionic emulsifiers, cationic emulsifiers, phospholipids.

[0024] In some embodiments, the external use product further contains an auxiliary material containing a modifying auxiliary material: a flavoring and / or a pigment.

[0025] In some embodiments, the internal use product further contains a food science acceptable auxiliary material containing a flavoring agent: one or more selected from the group consisting of fructooligosaccharides, lactose, steviol glycosides, xylitol, honey, sugar syrup, peppermint oil, instant tea powder, fruit juice powder, food flavoring, citric acid.

[0026] In some embodiments, the internal use product further contains a food science acceptable auxiliary material containing a dosage form auxiliary material: one or more selected from the group consisting of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methylcellulose, microcrystalline cellulose, silicon dioxide.

[0027] The beneficial effects of the present application are:

[0028] 1) The torreya grandis hydrolat can target skin flora and restore flora balance. When the concentration of torreya grandis hydrolat is 12.5%, the growth inhibition rate of propionibacterium acnes CCSM0331 is 50%, and the growth inhibition rate of staphylococcus epidermidis CCSM0287 is 5%, which can effectively inhibit the growth of propionibacterium acnes, while having little effect on the growth of staphylococcus epidermidis.

[0029] 2) The torreya grandis hydrolat has an antioxidant capacity of more than 85% and 90% for DPPH and ABTS+ free radical scavenging rates, respectively.

[0030] 3) The torreya grandis hydrolat is made from fresh torreya grandis aril, which has a more concentrated original flavor and aroma, higher nutrient content and active substance content, and can provide more active ingredients and effects compared to dried aril. In addition, compared to dried torreya grandis, fresh torreya grandis does not need to go through the tedious processing process of drying, which can save a lot of time and labor cost, improve production efficiency and economic benefit. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 The DPPH free radical scavenging capacity of each concentration of torreya grandis hydrolate;

[0032] Figure 2 The ABTS+ free radical scavenging capacity of each concentration of torreya grandis hydrolate;

[0033] Figure 3 The intervention test results of each concentration of torreya grandis hydrolate on propionibacterium acnes CCSM0331, respectively. DETAILED DESCRIPTION

[0034] The present application will be described in detail below in conjunction with the accompanying drawings and specific embodiments. The present embodiment is implemented on the premise of the technical solution of the present application, and gives a detailed implementation and specific operation process, but the protection scope of the present application is not limited to the following embodiments.

[0035] The purpose of the present application is to provide a torreya grandis hydrolate in the preparation of products.

[0036] In some embodiments, the torreya grandis hydrolate can be used to prepare products for preventing and / or treating acne.

[0037] In some embodiments, the torreya grandis hydrolate can be used to prepare products with antioxidant effects.

[0038] In some embodiments, the torreya grandis hydrolate can be used to prepare products with DPPH free radical and ABTS+ free radical scavenging capacity.

[0039] In some embodiments, the torreya grandis hydrolate can be used to prepare products for regulating skin flora balance.

[0040] In some embodiments, the torreya grandis hydrolate can be used to prepare products for inhibiting the growth of propionibacterium acnes.

[0041] In some embodiments, the torreya grandis hydrolate is obtained by water vapor distillation, condensation, oil-water separation of torreya grandis aril, and then filtration.

[0042] The specific steps of water vapor distillation are as follows: fresh torreya grandis aril is used as raw material, water is added and then distilled, the steam obtained by distillation is condensed by a condenser, and the condensed liquid collected by distillation is subjected to oil-water separation after high-speed centrifugation, the upper layer is torreya grandis essential oil, and the lower layer is torreya grandis hydrolate raw liquid. The torreya grandis hydrolate raw liquid is filtered to obtain torreya grandis hydrolate.

[0043] In some embodiments, the weight-to-volume ratio of the torreya grandis aril to water is 1:2-5 (Kg: L), where Kg is kilogram, a unit of weight, and L is liter, a unit of volume.

[0044] In some embodiments, in the water vapor distillation, the distillation temperature is 80-100°C, and the distillation time is 1-5h; preferably, the distillation temperature is 90-95°C, and the distillation time is 2.5-3h.

[0045] In some embodiments, the filtration is membrane filtration, preferably using a 0.3-0.7μm filter membrane, further preferably a 0.45μm filter membrane.

[0046] Torreya grandis Fort. et Lindl.), the wild Torreya tree produced in China, also known as Xiangyou, Yangjiaoyou, Xiaoyou, Yuyou, Yeshan, and so on; Torreya taxifolia Am. and Torreya californica Torrey produced in North America; and Torreya nucifera (L.) Sieb. et Zucc. produced in Japan.

[0047] In some specific embodiments, the Torreya is Torreya grandis Fort. et Lindl.

[0048] DPPH radical, which is also known as 2,2-diphenyl-1-picrylhydrazyl, is a kind of radical with deep purple color. Its core structure contains an ammonia atom and a benzene ring, which makes it react with other radicals to produce changes in organic matter. DPPH radical is usually used to determine the antioxidant performance of samples such as food and plant extracts. The mechanism of action is to capture its unpaired electron to form a stable double-electron product. In the reaction, the deep purple color of DPPH radical gradually fades, so that the reaction solution changes from purple to colorless. This reaction can be monitored by spectroscopy, and the content of antioxidant components in the sample can be quantitatively analyzed according to the change in absorbance.

[0049] ABTS (2,2-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging principle is to use the redox reaction ability of ABTS molecules to evaluate the free radical scavenging ability of antioxidant substances. When ABTS+ radical excites electron transition to a high energy state, it will emit blue fluorescence. However, if an antioxidant that can scavenge free radicals encounters ABTS+, it can promote chemical reactions, so that the electrons of ABTS+ are captured and reduced to unactivated ABTS molecules, thereby weakening or losing blue fluorescence. The stronger the free radical scavenging ability of antioxidant substances, the faster the rate of ABTS+ being reduced to ABTS, which is manifested as the greater the degree of decrease in fluorescence intensity. Therefore, the ABTS radical scavenging principle can be used to evaluate the antioxidant capacity of natural or synthetic compounds.

[0050] Propionibacterium acnes is a species of the genus Propionibacterium, which is the pathogenic bacteria causing acne. It is named for the production of propionic acid by fermenting glucose, and is a gram-positive bacillus, rod-shaped or slightly curved with uneven staining. It is often arranged in X, Y and V shapes, similar to the shape of pseudomonal bacillus, and is often filamentous in old culture, with high polymorphism.

[0051] In some embodiments, the concentration of the Torreya grandis seed extract is 20-100 mg / mL.

[0052] In some specific embodiments, the concentration of the Torreya grandis seed extract is preferably 1.562-25 mg / mL.

[0053] In some specific embodiments, the concentration of the Torreya grandis seed extract is further preferably 25 mg / mL.

[0054] In some specific embodiments, the product with the above-mentioned efficacy is an external product or an internal product.

[0055] The external product is selected from skin care products, cosmetics, hair care products, preferably skin care products and / or cosmetics;

[0056] The internal product is selected from solid beverages, liquid beverages, dairy products, seasoning powders, nutritional products, and internal medical health care products.

[0057] In some embodiments, the external product is selected from emulsions, gels, creams, microneedle patches, and masks.

[0058] In some embodiments, the external product further contains an auxiliary material containing a preservative ingredient selected from one or more of methylparaben, ethylparaben, propylparaben, formaldehyde releasers, methylchloroisothiazolinone, methylisothiazolinone, phenoxyethanol, imidazolidinyl urea, isothiazolinone, phenol, benzoic acid, benzyl alcohol, potassium sorbate, bronopol, p-hydroxyacetophenone, chlorphenesin, triclosan, quaternary ammonium salt-15, dehydroacetic acid and its salts.

[0059] In some embodiments, the external product further contains an auxiliary material containing an emulsifying ingredient selected from one or more of polyethylene glycols, glycerol esters, sugar derivatives, anionic emulsifiers, cationic emulsifiers, and phospholipids.

[0060] In some embodiments, the external product further contains an auxiliary material containing a modifying auxiliary material selected from fragrances and / or pigments.

[0061] The internal product of the present application can include all foods in the conventional sense, and can be used interchangeably with the terms known in the art, such as functional foods and health functional foods.

[0062] When the torreya grandis hydrolate of the present application is used as a food additive, it can be directly added or used with other foods or food ingredients, and can be appropriately used according to conventional methods.

[0063] In some embodiments, the internal product further contains a food science acceptable auxiliary material containing a flavoring agent: one or more selected from the group consisting of fructooligosaccharide, lactose, stevioside, xylitol, honey, sugar syrup, peppermint oil, instant tea powder, fruit juice powder, food essence, citric acid.

[0064] In some embodiments, the internal product further contains a food science acceptable auxiliary material containing a dosage form auxiliary material: one or more selected from the group consisting of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methyl cellulose, microcrystalline cellulose, silicon dioxide.

[0065] The present application is further illustrated by the following examples, but the present application is not limited to the scope of the examples. The raw materials or processing techniques in the following examples, unless otherwise specified, are all conventional commercially available raw material products or conventional processing techniques in the art.

[0066] Experimental strains:

[0067] Staphylococcus epidermidis CCSM0287 and Propionibacterium acnes CCSM0331 were isolated from healthy human facial skin.

[0068] Culture medium: Trypticase soybean agar blood culture medium (TSA): Trypticase 15 g / L, soybean papain hydrolysate 5 g / L, sodium chloride 5 g / L, agar 15 g / L, 121°C high pressure sterilization for 15 min, cooled to about 50°C, then added 5% sterile defibrillated sheep blood, mixed evenly, and poured into plates.

[0069] Trypticase soybean broth (TSB): Trypticase 15 g / L, soybean papain hydrolysate 5 g / L, sodium chloride 5 g / L, 121°C high pressure sterilization for 15 min, ready for use.

[0070] Example 1 Preparation of torreya grandis hydrolate

[0071] Take fresh torreya grandis aril 50 kg and wash it clean. Place the washed fresh torreya grandis aril in a 500 L stainless steel distillation column, add 250 L deionized water, and distill at a temperature of 90-95°C for 2.5-3h. The condensate collected by distillation is subjected to high-speed centrifugation and then oil-water separation. The upper layer is torreya grandis essential oil, and the lower layer is torreya grandis hydrolate raw liquid. The torreya grandis hydrolate raw liquid is filtered with a 0.45μm membrane to obtain filtered torreya grandis hydrolate, which is the torreya grandis hydrolate.

[0072] Example 2 DPPH free radical scavenging test of torreya grandis hydrolate

[0073] Control group: Take 1 mL of 2×10 -4 A mol / L DPPH solution was prepared by adding water to a final volume of 4 mL. After incubation at room temperature in the dark for 30 min, the absorbance was measured at 517 nm and recorded as a0. For sample solutions, 1 mL of DPPH solution was mixed with 1 mL of 20, 40, 60, 80, and 100 mg / mL Torreya grandis hydrosol, respectively. Water was then added to a final volume of 4 mL. After incubation at room temperature in the dark for 30 min, the absorbance of the different concentrations of Torreya grandis hydrosol was measured and recorded as a. A vitamin C concentration of the same mass was used as a positive control. Each sample was repeated three times. The DPPH free radical scavenging rate (%) was calculated as (a0 - a) / a0 × 100%.

[0074] The test results are as follows Figure 1 As shown, from Figure 1 It can be seen that the scavenging ability of Torreya grandis hydrosol on DPPH free radicals increases with the increase of mass concentration, and the scavenging rate can reach up to 85.23±3.6% (P<0.001). The results show that Torreya grandis hydrosol has a good antioxidant and free radical scavenging ability.

[0075] Example 3: Test on ABTS+ free radical scavenging effect of Torreya grandis hydrosol

[0076] Equal volumes of 7 mmol / L ABTS were mixed with 2.6 mmol / L potassium persulfate solution and incubated at room temperature in the dark for 12–16 h to obtain ABTS+ stock solution. Before use, the solution was diluted with anhydrous ethanol to obtain an absorbance of 0.70 ± 0.02 at 734 nm, thus obtaining the ABTS+ working solution. Control solution: A 10 mL test tube was filled with 1.0 mL of ABTS+ working solution and 3.0 mL of anhydrous ethanol to a final volume of 4.0 mL. The solution was allowed to stand for 30 min, and the absorbance was measured at 734 nm and recorded as a0. Sample solution: Several 10 mL test tubes were filled with 1 mL of 20, 40, 60, 80, and 100 mg / mL Torreya grandis hydrosol, respectively, mixed with 2.0 mL of anhydrous ethanol, and then ABTS+ working solution was added to a final volume of 4.0 mL. The solution was allowed to stand in the dark for 30 min, and the absorbance was measured at 734 nm and recorded as a. The experiment used the same mass concentration of V... C As a positive control, each sample was repeated 3 times. ABTS free radical scavenging rate (%) = (a0-a) / a0×100%.

[0077] The test results are as follows Figure 2 As shown, with increasing concentration, the positive control V... CThe ABTS+free radical scavenging capacity of Torreya grandis pure distillate was enhanced with the increase of the concentration of Torreya grandis pure distillate, and reached 91.26±4.8% at the highest concentration. The results showed that Torreya grandis pure distillate had good antioxidant capacity to scavenge free radicals.

[0078] Example 4: Effect of Torreya grandis pure distillate on the proliferation of Staphylococcus epidermidis CCSM0287

[0079] 1) Preparation of bacterial suspension of Staphylococcus epidermidis CCSM0287: The preserved Staphylococcus epidermidis CCSM0287 freeze-dried tube of milk powder was taken out from the -80℃ refrigerator, and the milk powder was picked up with a disposable inoculation ring and inoculated on a TSA plate. After the three-zone streaking method was used, the plate was placed in a 37℃ constant temperature incubator for aerobic culture for 16-24h to obtain a first generation activated plate. Single colonies were picked from the first generation plate and inoculated on a second generation plate, which was placed in a 37℃ constant temperature incubator for aerobic culture for 16-24h. Single colonies of Staphylococcus epidermidis activated for two generations were inoculated in a shaking tube containing 5mL of TSB liquid medium, which was placed in a 37℃ shaking incubator at a speed of 170r / min for 16h. The OD600 value of the bacterial suspension was adjusted to 0.1, and the bacterial suspension was diluted to 10 600 in the range of 0.55-0.56, standby.

[0080] 2) Determination of the growth of the strain by using the half dilution method

[0081] Half dilution with five dilution gradients was carried out in a sterile 96-well plate, and four duplicate wells were set for each group. First, 100μL of Torreya grandis pure distillate with a concentration of 50mg / mL and 100μL of sterile TSB medium were added to the first well and mixed evenly. Then, 50μL of the solution in the first well was added to the second well, and 50μL of sterile TSB medium was added to fully mix and repeat the above operation to the fifth well. Finally, 50μL of sterile TSB medium was added to the fifth well. The concentrations of Torreya grandis pure distillate obtained were 25mg / mL, 12.5mg / mL, 6.25mg / mL, 3.125mg / mL and 1.562mg / mL.

[0082] Experimental group: 100μL of sterile TSB medium + 50μL of each concentration of sample + 50μL of 10 6 cfu / mL of Staphylococcus epidermidis bacterial suspension; experimental blank group: 50μL of each concentration of sample + 150μL of sterile TSB medium; growth control group: 50μL of 10 6 cfu / mL of bacterial suspension + 150μL of sterile TSB medium; sterile control group: 200μL of sterile TSB medium; the 96-well plate was incubated at 37℃ for 24h, and the OD 600 value was measured using a microplate reader, and the inhibition rate was calculated.

[0083] Inhibition rate = 1 - (OD value of experimental group - OD value of experimental blank group) / (OD value of growth control well - OD value of sterile control well) x 100%.

[0084] (3) Experimental results

[0085] The results are shown in Table 1. The inhibition rate of Torreya grandis pure distillate was less than 10% at a concentration range of 1.562-25 mg / mL, indicating that Torreya grandis pure distillate had little effect on Staphylococcus epidermidis.

[0086] Table 1: Inhibition rate (%) of Torreya grandis pure distillate on Staphylococcus epidermidis CCSM0287

[0087]

[0088] Example 5: Effect of Torreya grandis pure distillate on the proliferation of Propionibacterium acnes CCSM0331

[0089] 1) Preparation of bacterial suspension of Propionibacterium acnes CCSM0331: The preserved Propionibacterium acnes strain milk powder freeze-dried tube was taken out from the -80°C refrigerator, and the milk powder was picked up with a disposable inoculation loop and streaked on a TSA plate. After streaking with a three-zone streaking method, it was placed in a 37°C constant temperature incubator for anaerobic culture for 48-60 h to obtain a first generation activated plate. Single colonies were picked from the colonies grown on the first generation plate and streaked on a second generation plate, which was placed in a 37°C constant temperature incubator for anaerobic culture for 48-60 h. Single colonies of the two-generation activated Propionibacterium acnes were inoculated into a test tube containing 5 mL of TSB liquid medium, and incubated in a 37°C incubator for 48-60 h. The bacterial suspension was adjusted to an OD600 of 0.1-0.2. 600 About 0.3, standby.

[0090] 2) Determination of the growth of the strain by half dilution method:

[0091] Half dilution was performed in a sterile 96-well plate with five dilution gradients. Four replicate wells were set up for each group. First, 100 μL of Torreya grandis pure distillate with a concentration of 50 mg / mL and 100 μL of sterile TSB medium were added to the first well and mixed evenly. Then, 50 μL of the solution from the first well was added to the second well, and 50 μL of sterile TSB medium was added and mixed thoroughly. The above operation was repeated to the fifth well, and 50 μL of sterile TSB medium was added to the fifth well. Torreya grandis pure distillate with concentrations of 25 mg / mL, 12.5 mg / mL, 6.25 mg / mL, 3.125 mg / mL, and 1.562 mg / mL was obtained.

[0092] Experimental group: 100 μL of sterile TSB medium + 50 μL of each concentration of sample + 50 μL of 10 6C. acnes bacteria suspension of 10 cfu / mL; experimental blank group: 50 μL of each concentration sample + 150 μL sterile TSB medium; growth control group: 50 μL 10 6 C. acnes bacteria suspension of 10 cfu / mL + 150 μL sterile TSB medium; sterile control group: 200 μL sterile TSB medium; the 96-well plate was cultured anaerobically at 37°C for 48 h, and the OD 600 value was measured using an enzyme-labeled instrument to calculate the inhibition rate.

[0093] Inhibition rate = 1 - (experimental group OD value - experimental blank group OD value) / (growth control well OD value - sterile control well OD value) x 100%.

[0094] (3) Experimental results

[0095] The results are shown in Table 1. Figure 3 As shown in Table 1, the torreya grandis hydrolate significantly inhibited C. acnes CCSM0331 at multiple concentrations (P < 0.01), and the growth inhibition rate of C. acnes was about 50% at a concentration of 12.5 mg / mL, and the growth inhibition rate of C. acnes was more than 90% at a maximum concentration of 25 mg / mL, indicating that the torreya grandis hydrolate can effectively inhibit the growth of C. acnes.

[0096] In summary, the torreya grandis hydrolate can well regulate the skin flora balance of acne patients, reduce the abundance of C. acnes, and has little effect on S. epidermidis, can regulate the skin flora balance, and prevent and treat acne.

[0097] The above examples are only preferred embodiments of the present application, and are not any form and substantial limitation of the present application. It should be noted that for ordinary skilled persons in the art, without departing from the present application, a number of improvements and supplements can also be made, which should be considered as the protection scope of the present application.

Claims

1. Use of torreya grandis pure distillate in the preparation of products, 1) products for preventing and / or treating acne; 2) products with antioxidant effects; 3) products with anti-inflammatory effects; 4) products with DPPH free radical and ABTS+ free radical scavenging capacity; 5) products for regulating skin flora balance; 6) products for inhibiting the growth of propionibacterium acnes.

2. Use according to claim 1, wherein The torreya grandis pure distillate is obtained by water vapor distillation, condensation, oil-water separation, and then filtration of torreya grandis aril.

3. Use according to claim 2, wherein the compound is ###0002### In the water vapor distillation, the weight-volume ratio of torreya grandis aril to water is 1:2-5 (Kg:L).

4. Use according to claim 2 or 3, wherein the compound is ###0002### In the water vapor distillation, the distillation temperature is 80-100℃, and the distillation time is 1-5h; preferably, the distillation temperature is 90-95℃, and the distillation time is 2.5-3h.

5. Use according to any one of claims 2 to 4, wherein the compound is of formula (I) ###0001### (I) or a pharmaceutically acceptable salt thereof. The filtration is membrane filtration, preferably using a 0.3-0.7μm filter membrane, and further preferably a 0.45μm filter membrane.

6. Use according to any one of claims 1 to 4, wherein The concentration of the torreya grandis pure distillate is 20-100mg / mL, preferably 1.562-25mg / mL, and further preferably 25mg / mL.

7. Use according to any one of claims 1 to 6, wherein The products are external use products or internal use products, the external use products are selected from skin care products, cosmetics, hair care products; the internal use products are selected from solid beverages, liquid beverages, dairy products, seasoning powders, nutritional products, and internal use medical health care products.

8. Use according to claim 7, wherein the compound is ###0002### The external use products are selected from emulsions, gels, creams, microneedle patches, and masks.

9. Use according to claim 7 or 8, characterized in that, The external use products further contain excipients, which have any one or more of the following characteristics: 1) containing a preservative ingredient: the preservative ingredient is selected from one or more of nipagin methyl ester, nipagin ethyl ester, nipagin propyl ester, formaldehyde releasers, methyl chloroisothiazolinone, methyl isothiazolinone, phenoxyethanol, imidazolidinyl urea, isothiazolinone, phenol, benzoic acid, benzyl alcohol, potassium sorbate, broopal, p-hydroxyacetophenone, chlorphenesin, triclosan, quaternary ammonium salt-15, dehydroacetic acid and its salts; 2) containing an emulsifying ingredient: the emulsifying ingredient is selected from one or more of polyethylene glycol, glycerol ester, sugar derivative, anionic emulsifier, cationic emulsifier, phospholipid; 3) containing a modified excipient: the modified ingredient is selected from essence and / or pigment.

10. The use according to claim 7, wherein the compound is ###0002### The internal use products further contain food science acceptable excipients, which have any one or more of the following characteristics: 1) containing a flavoring agent, the flavoring agent is selected from one or more of oligofructose, lactose, stevioside, xylitol, honey, sugar syrup, peppermint oil, instant tea powder, fruit juice powder, food essence, citric acid; 2) containing a dosage form excipient, the dosage form excipient is selected from one or more of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methyl cellulose, microcrystalline cellulose, silicon dioxide.

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