Production device and process of high-load whole-process nitrifying bacterial agent

Abstract

The invention discloses a production device of a high-load whole-course nitrifying bacterial agent. The production device comprises a fermentation propagation tank body and a first air compressor, a first heating rod, a first temperature sensor, a pH sensor and an ammonia nitrogen sensor which are connected with a PLC (Programmable Logic Controller) control system as well as a filler frame in which a non-biofilm-forming hydrophilic polyurethane porous filler and a hydrophilic polyurethane porous filler loaded with activated AOB and NOB are added are arranged in the fermentation propagation tank body; a sodium carbonate liquid supplementing pump, a sodium bicarbonate liquid supplementing pump and an ammonium chloride liquid supplementing pump are connected to the upper side of a liquid supplementing opening in the top end of the fermentation and propagation tank body, are connected with the PLC control system and are interlocked with the pH sensor and the ammonia nitrogen sensor under the control of the PLC control system; the lower side of the liquid supplementing opening is connected with a gas-liquid distribution pipe; the first air compressor is connected with an air inlet pipe, the air inlet pipe penetrates into the gas-liquid distribution pipe from the bottom surface of the gas-liquid distribution pipe and is hermetically connected with the bottom surface of the gas-liquid distribution pipe, and a hole is formed in the side surface of the air inlet pipe in the gas-liquid distribution pipe.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of water treatment, and particularly relates to a production device and process of high-load full-process nitrifying bacteria agent. BACKGROUND

[0002] With the rapid development of industry, the discharge of industrial wastewater has increased dramatically, and nitrogen pollution has become a problem in water management. Autotrophic nitrifying bacteria, including ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), are crucial in biological denitrification.

[0003] Currently, the existing culture technology of autotrophic nitrifying bacteria cannot balance the stability of mixed culture, low nitrite accumulation and regulation universality, which seriously restricts the efficient industrial production of autotrophic nitrifying bacteria agent. Specifically, the culture is first limited by inherent physiological characteristics: long generation time and slow growth rate, making it difficult to achieve high-density mixed culture in the same reactor. Existing technologies generally use step-by-step culture mode (such as the segmented fermentation process of CN108424862B), resulting in complex process, large land occupation and high cost. In addition, traditional culture enrichment methods also have product defects. Although the activated sludge enrichment method (CN106754483B) can short-term increase the AOB concentration, the bacteria agent has many impurities and poor stability, and the adsorption technology (CN101709278A, CN102757913A) relies on chemical flocculants, which has biological toxicity risk. More importantly, the current regulation strategy has serious blind spots, for example: most methods only use pH or nitrate concentration as an indicator (such as the consortium enrichment process of CN109055285A), ignoring the inhibitory effect of free ammonia and nitrite accumulation on the bacterial community; the intermittent feeding scheme (CN10639613B) can improve the ammonia oxidation rate, but it leads to a dramatic increase in nitrite concentration (>100 mg / L) with the reaction process, further inhibiting the metabolic activity of NOB. Therefore, a new technical solution is needed to overcome the three major problems of poor culture stability, high nitrite accumulation and low regulation universality of the existing expansion culture method, to achieve low-nitrite accumulation and high-density nitrifying bacteria mixed expansion culture, and to efficiently treat high-concentration ammonia-nitrogen wastewater. SUMMARY

[0004] The existing autotrophic nitrifying bacteria agent expansion technology faces three core defects: ① The bacteria agent enriched by the activated sludge method has many impurities and poor microbial stability; ② The step-by-step expansion method is complex and occupies a large area; and ③ The accumulation of nitrite nitrogen leads to low processing efficiency. Therefore, the present application first provides a simple, low-cost and easy-to-operate nitrifying bacteria expansion device, which reduces the occupied area by more than 50%, and develops an AOB-NOB collaborative expansion process based on carrier partitioning membrane, which realizes the simultaneous production of low nitrite nitrogen accumulation and high ammonia oxidation rate of nitrifying bacteria agent in a single tank through step-by-step ammonia nitrogen domestication and dissolved oxygen gradient regulation. At the same time, a dynamic compensation mechanism of ammonia nitrogen concentration-pH signal interlocking is established, and the nutrient addition and environmental parameter adjustment are automatically executed by the PLC system, breaking the dependence on artificial experience in traditional methods, and realizing the rapid, stable and batch production of high-purity nitrifying bacteria agent under the premise of low nitrite nitrogen accumulation.

[0005] In a first aspect, the present application provides a high-load full-process nitrifying bacteria production device, comprising a fermentation expansion tank and a first air compressor; The fermentation expansion tank is provided with a first heating rod, a first temperature sensor, a pH sensor, an ammonia nitrogen sensor and a filler frame; the first heating rod, the first temperature sensor, the pH sensor and the ammonia nitrogen sensor are connected to a PLC control system; the filler frame is filled with unmembrane-attached hydrophilic polyurethane porous filler and hydrophilic polyurethane porous filler loaded with activated AOB and NOB; The fermentation expansion tank is provided with an upper water outlet and a lower water outlet, and the lower water outlet is close to the bottom of the fermentation expansion tank; the top of the fermentation expansion tank is provided with a liquid supplementing port; the upper side of the liquid supplementing port is connected to a sodium carbonate liquid supplementing barrel, a sodium bicarbonate liquid supplementing barrel and an ammonium chloride liquid supplementing barrel through a sodium carbonate liquid supplementing pump, a sodium bicarbonate liquid supplementing pump and an ammonium chloride liquid supplementing pump respectively; the sodium carbonate liquid supplementing pump, the sodium bicarbonate liquid supplementing pump and the ammonium chloride liquid supplementing pump are connected to the PLC control system and form interlocking with the pH sensor and the ammonia nitrogen sensor under the control of the PLC control system; the lower side of the liquid supplementing port is connected to a gas-liquid distribution pipe, and the gas-liquid distribution pipe extends into the filler frame and has a side hole corresponding to the filler frame; The first air compressor is connected to an air inlet pipe, the air inlet pipe penetrates into the gas-liquid distribution pipe from the bottom surface of the gas-liquid distribution pipe and is sealingly connected to the bottom surface of the gas-liquid distribution pipe, and the air inlet pipe in the gas-liquid distribution pipe has a side hole.

[0006] Preferably, the high-load full nitrification bacteria agent production device further comprises a second air compressor, a microporous aeration disc is arranged below the filler frame in the fermentation expansion tank, the microporous aeration disc is connected to the second air compressor, the second air compressor is connected to the PLC control system and forms interlocking with the ammonia nitrogen sensor under the control of the PLC control system. That is, the PLC control system can control the working condition of the second air compressor according to the detection result of the ammonia nitrogen sensor. For example, when the detection result of the ammonia nitrogen sensor is greater than the set threshold value (for example, the ammonia nitrogen concentration is 200 mg / L, etc.), the PLC control system controls the second air compressor to start, and the microporous aeration disc is aerated; when the detection result of the ammonia nitrogen sensor does not exceed the set threshold value (for example, the ammonia nitrogen concentration is 200 mg / L, etc.), the PLC control system controls the second air compressor to be closed, and the microporous aeration disc stops aeration. The second air compressor and the microporous aeration disc can be used as a supplementary aeration device to ensure that there is sufficient dissolved oxygen when the ammonia nitrogen concentration in the fermentation expansion tank is greater than 200 mg / L.

[0007] Preferably, the side holes of the gas-liquid distribution pipe corresponding to the filler frame are sawtooth-shaped sieve holes with a pore size of 0.5-2 cm, for example, 0.5-1 cm, etc., which facilitates the diffusion of liquid into the fermentation expansion tank. The side holes of the gas inlet pipe located in the gas-liquid distribution pipe are dense small gas outlets. The gas inlet pipe is connected to the first air compressor. When the first air compressor is turned on, overflow flows through the side holes of the gas-liquid distribution pipe, breaks through the sieve sawtooth, forms tiny bubbles, and flows with the liquid phase to the tank, increasing the dissolved oxygen in the culture solution and simultaneously uniformizing the water quality.

[0008] The high-load full nitrification bacteria agent production device can be opened at the top of the fermentation expansion tank, facilitating the removal of the filler frame and the replacement of new fillers. The filler frame can be a stainless steel mesh cylindrical frame with an outer diameter slightly smaller than the inner diameter of the fermentation expansion tank, used to fix the fillers.

[0009] The specific surface area of the uncoated hydrophilic polyurethane porous filler is preferably 300-800 m 2 / m 3 .

[0010] The high-load full nitrification bacteria agent production device, the preparation process of the hydrophilic polyurethane porous filler loaded with activated AOB and NOB preferably comprises the following steps: 1) inoculate AOB into activated culture medium with inoculation amount of 2-10 g / L (for example 5 g / L, etc.), add hydrophilic polyurethane porous filler, and shake culture; the pH value of the activated culture medium is 7.7-7.9, and the components include: 0.1-0.3 g / L (for example 0.2 g / L, etc.) NH4Cl, 0.3-0.5 g / L K2HPO4, 0.1 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 0.1-0.3 g / L CaCO3, 1-2 g / L Na2CO3; 2) after the ammonia nitrogen conversion rate in the culture medium of step 1) reaches 30%-50%, add NOB with a mass ratio of NOB:AOB of 1-2:1 (for example 1.5:1, etc.), continue to shake culture for 24-48 h, and the rotation speed of the shake culture meets the dissolved oxygen requirement for the growth of NOB, to obtain hydrophilic polyurethane porous filler loaded with activated AOB and NOB.

[0011] In the activated culture medium of the present application, calcium carbonate is used, and AOB releases acid when growing, thereby dissolving part of the calcium carbonate in the activated culture medium for use, which avoids the need for frequent addition of carbon source when culturing in a small system, and also avoids the pH of the system from fluctuating sharply due to the addition of too much soluble carbon source (such as sodium acetate) at one time.

[0012] In step 1), the specific surface area of the added hydrophilic polyurethane porous filler is 300-800 m 2 / m 3 .

[0013] Preferably, in step 1), the added volume of the hydrophilic polyurethane porous filler is 30%-50% of the volume of the activated culture medium, for example 40%, etc.

[0014] In step 1), the temperature of the shake culture is preferably 30±2℃.

[0015] In step 1), the rotation speed of the shake culture is preferably 100-120 rpm.

[0016] In step 1), the time of the shake culture is preferably 24-48 h.

[0017] In step 2), the temperature of the shake culture is 30±2℃.

[0018] The present application uses a hydrophilic polyurethane porous filler, which has a hydrophilic layer on the surface, and the specific surface area thereof is 300-800 m 2 / m 3The hydrophilic polyurethane porous filler can be used to control the dissolved oxygen in different areas of the carrier, so that a low dissolved oxygen area suitable for the growth of AOB is formed inside, the load of AOB is promoted, and the biomass of AOB is rapidly increased; meanwhile, the hydrophilic group combines with the extracellular polymer secreted by AOB itself, so that NOB can stay on the surface of AOB, and a gradient culture of AOB and NOB is formed. Inside the hydrophilic polyurethane porous filler, AOB preferentially utilizes ammonia and a low-oxygen environment for growth. Outside the hydrophilic polyurethane porous filler, NOB can utilize nitrite produced by AOB and higher oxygen for growth.

[0019] The production device of the high-load full nitrification bacteria agent, preferably, the sodium carbonate liquid supplementing barrel is provided with a second heating rod and a second temperature sensor, and the second heating rod and the second temperature sensor are connected with the PLC control system. The second heating rod and the second temperature sensor arranged in the sodium carbonate liquid supplementing barrel can effectively improve the concentration of the sodium carbonate solution, facilitate rapid feeding, and avoid excessive liquid supplementing into the fermentation expansion tank.

[0020] In a second aspect, the application provides application of the production device of the high-load full nitrification bacteria agent in the production of the high-load full nitrification bacteria agent.

[0021] In a third aspect, the application provides a production process of the high-load full nitrification bacteria agent, which adopts the production device of the high-load full nitrification bacteria agent. The production process of the high-load full nitrification bacteria agent comprises the following steps: The expansion culture medium is added into the fermentation expansion tank, and the PLC control system controls the working condition of the first heating rod according to the detection result of the first temperature sensor, so as to control the temperature in the fermentation expansion tank at 30±2℃; the pH value of the expansion culture medium is 7.4-7.6, and the components include: 0.08-0.20 g / L NH4Cl, 0.425-0.625 g / L NaHCO3, 0.03-0.05 g / L Na2CO3, 0.078 g / L NaH2PO4, 0.1 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 0.03 g / L CaCl2·2H2O, and 1 mL / L trace element concentrate; the pH value of the trace element concentrate is 6.0-6.5, and the components include: 0.5 g / L FeCl2·4H2O, 0.011 g / L MnCl2·4H2O, 0.07 g / L ZnCl2, 0.006 g / L H3BO3, 0.036 g / L Na2MoO4·2H2O, 0.014 g / L NiCl2·6H2O, and 0.002 g / L CuCl2·2H2O; The un-membrane-attached hydrophilic polyurethane porous filler and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB are added into the filler frame. Turning on the first air compressor (and / or the second air compressor, for example: when the detection result of the ammonia nitrogen sensor is greater than the set threshold value, for example 200 mg / L, the second air compressor can be turned on at the same time), so that the dissolved oxygen concentration of the culture medium in the fermentation expansion tank is 3-6 mg / L; The ammonia nitrogen concentration is gradually increased in the stepwise domestication, and the minimum threshold value and the maximum threshold value of the ammonia nitrogen sensor are set in each domestication stage (it can be understood that the maximum threshold value is higher than the minimum threshold value). The minimum threshold value and the maximum threshold value of the pH sensor are also set in the stepwise domestication process (it can be understood that the maximum threshold value is higher than the minimum threshold value). The PLC control system controls the operation of the sodium bicarbonate solution pump and the ammonium chloride solution pump according to the triggering condition of the ammonia nitrogen sensor, and then controls the solution of sodium bicarbonate and ammonium chloride, controls the operation of the sodium carbonate solution pump according to the triggering condition of the pH sensor, and then controls the solution of sodium carbonate, so that the ammonia nitrogen and pH of the culture medium in the fermentation expansion tank are within the set threshold value range during the domestication process.

[0022] For example, when the pH of the culture medium decreases to the minimum threshold value of the pH sensor, the PLC control system controls the sodium carbonate solution pump to be turned on to perform sodium carbonate solution, until the pH of the culture medium increases to the maximum threshold value of the pH sensor, at this time the PLC control system controls the sodium carbonate solution pump to be turned off to stop the sodium carbonate solution, so as to realize the automatic control of the pH of the culture medium and ensure that the pH of the culture medium is always within the set threshold value range.

[0023] The above-mentioned pH control process can run through the entire process of stepwise domestication. One pH sensor minimum threshold value and one pH sensor maximum threshold value can be set in the entire process of stepwise domestication, or any number of pH sensor minimum threshold values and maximum threshold values that are completely the same, partially the same or completely different can be set in any domestication stage.

[0024] The culture medium ammonia nitrogen control logic can be similar to the culture medium pH control logic, for example, when the ammonia nitrogen concentration of the culture medium decreases to the minimum threshold value of the ammonia nitrogen sensor, the PLC control system controls the sodium bicarbonate solution pump and the ammonium chloride solution pump to be turned on (specifically according to the set proportion) to perform sodium bicarbonate and ammonium chloride solution, until the ammonia nitrogen concentration of the culture medium increases to the maximum threshold value of the ammonia nitrogen sensor, at this time the PLC control system controls the sodium bicarbonate solution pump and the ammonium chloride solution pump to be turned off to stop the sodium bicarbonate and ammonium chloride solution, so as to realize the automatic control of the ammonia nitrogen concentration of the culture medium and ensure that the ammonia nitrogen concentration of the culture medium is always within the set threshold value range.

[0025] The ratio of the total volume of the un-membrane hydrophilic polyurethane porous filler and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB in the filler frame to the total volume of the culture medium in the fermentation expansion tank is 30-50:100, for example, 40:100, etc.

[0026] The ratio of the un-membrane hydrophilic polyurethane porous filler to the hydrophilic polyurethane porous filler loaded with activated AOB and NOB in the filler frame is preferably 1-3:1.

[0027] The dissolved oxygen concentration in the fermentation expansion tank can be controlled by adjusting the aeration pressure of the first air compressor and / or the second air compressor.

[0028] During the stepwise acclimation process, the culture medium is preferably periodically / irregularly discharged through the upper and / or lower drainage outlets to prevent the overflow of the culture medium in the fermentation expansion tank. For example, the aeration can be stopped for 3-6 hours every 20-40 hours of operation, and after the suspended bacteria are fully settled, the supernatant is discharged by turning on the drainage pump at the drainage outlet.

[0029] During the stepwise acclimation process, the ammonia nitrogen concentration is preferably increased by 10-50 mg / L at each level.

[0030] The minimum threshold value of the ammonia nitrogen sensor at each acclimation stage is preferably 20-120 mg / L.

[0031] The minimum threshold value of the ammonia nitrogen sensor at each acclimation stage can be the same, partially the same, partially different, or completely different.

[0032] The maximum threshold value of the ammonia nitrogen sensor at each acclimation stage is preferably 100-350 mg / L, and starting from the second acclimation stage, the maximum threshold value of the ammonia nitrogen sensor at any acclimation stage is higher than or equal to the maximum threshold value of the ammonia nitrogen sensor at the previous acclimation stage.

[0033] Preferably, in any acclimation stage, when the ammonia nitrogen concentration decreases to the minimum threshold value of the ammonia nitrogen sensor, the PLC control system controls the sodium bicarbonate and ammonium chloride liquid supplement pumps to be turned on for sodium bicarbonate and ammonium chloride liquid supplement, until the ammonia nitrogen concentration increases to the maximum threshold value of the ammonia nitrogen sensor at the next acclimation stage, at which time the PLC control system controls the sodium bicarbonate and ammonium chloride liquid supplement pumps to be turned off to stop sodium bicarbonate and ammonium chloride liquid supplement and enter the next acclimation stage.

[0034] The input amount of ammonium chloride and sodium bicarbonate can be adjusted by the flow rate control of the ammonium chloride and sodium bicarbonate makeup pumps, and the ratio will significantly affect the ammonia nitrogen degradation rate and the accumulation of nitrite nitrogen. In some preferred examples, when the sodium bicarbonate and ammonium chloride makeup solutions are added, the ratio of the volume of the sodium bicarbonate solution to the volume of the ammonium chloride solution is 2.5, the mass concentration of the ammonium chloride solution is 32%, and the mass concentration of the sodium bicarbonate solution is 9%.

[0035] The mass concentration of the sodium carbonate solution can be 20% to 30%, and when the second heating rod is turned on, the mass concentration of the sodium carbonate solution is preferably 30%.

[0036] In the production process of the high-load full-nitrification bacteria agent, the minimum threshold value triggered by the pH sensor is preferably from 7.2 to 7.8.

[0037] In the production process of the high-load full-nitrification bacteria agent, the maximum threshold value triggered by the pH sensor is preferably from 8.0 to 8.8.

[0038] In the production process of the high-load full-nitrification bacteria agent, the accumulation of nitrous nitrogen in the stepwise domestication process is not more than 30 mg / L.

[0039] In the production process of the high-load full-nitrification bacteria agent, preferably, in the stepwise domestication process, the ammonia nitrogen oxidation rate AOR is greater than or equal to 400 mg NH4 + -N / (g·h) or when the concentration of nitrate nitrogen in the fermentation expansion tank is higher than 12000 mg / L, the production of the high-load full-nitrification bacteria agent is completed.

[0040] Compared with the prior art, the present application has the following beneficial effects: 1. The present application establishes a sludge-free pure bacteria culture system, the microbial expansion process can be continuously carried out, the seed bacteria are added once, and the nutrient agent is continuously added, which greatly improves the denitrification efficiency and survival rate of microorganisms, greatly reduces the production cost, and is easy to operate.

[0041] 2. The pure bacteria (AOB+NOB) are used for compound inoculation, and the hydrophilic polyurethane porous filler is used for realizing partitioned membrane formation, which breaks through the site restriction of single-step culture of single bacteria, realizes the stability and high-efficiency synergistic effect of different functional microorganisms in the same reactor, effectively solves the problem that the difference between microorganisms is difficult to realize stability and efficient removal of pollutants in the same reactor in the traditional method; at the same time, the AOB in the inner layer of the carrier can form metabolic products with the NOB in the outer layer for synergistic metabolism, solving the problem of microbial competition inhibition in the traditional method, and the ammonia oxidation rate is increased by more than 10 times compared with the existing research.

[0042] 3. The step-by-step ammonia nitrogen concentration domestication process is implemented, the efficient activation and growth of nitrifying bacteria are realized through the adjustment of ammonia nitrogen concentration with gradient increase, the metabolic potential of nitrifying bacteria is activated, and the treatment efficiency of the system on high-concentration ammonia nitrogen wastewater is effectively improved, thereby solving the problem that high-concentration ammonia nitrogen wastewater cannot be efficiently treated in the prior art.

[0043] 4. The carrier partitioning membrane formation-parameter multi-dimension (ammonia nitrogen-pH-dissolved oxygen) interlocking regulation strategy is adopted, nutrients are dynamically supplemented based on real-time ammonia nitrogen / pH data, and the dissolved oxygen is simultaneously adjusted, specifically: based on the inner and outer partitioning of the filler, the dissolved oxygen is automatically adjusted; meanwhile, the microporous aeration disc is automatically started when the ammonia nitrogen concentration is high, so that the dissolved oxygen is improved, which is helpful to avoid the biological degradation inhibited by ammonia nitrogen under the condition of high ammonia nitrogen concentration. The present application realizes the partitioning culture of microorganisms, the internal low-oxygen zone protects AOB from the impact of free ammonia, and the external high-oxygen zone accelerates the degradation of nitrite nitrogen by NOB, thereby breaking through the bottleneck that the environmental control is disconnected from the metabolism of the bacterial flora in the traditional method, avoiding the limitation of artificial regulation required by industrial culture, significantly improving the universality of the culture method, and realizing the intelligent operation of the system. BRIEF DESCRIPTION OF DRAWINGS

[0044] Figure 1 It is a structure schematic diagram of the production device of the high-load full-process nitrifying bacteria agent in the specific embodiment.

[0045] Figure 2 It is an ammonia nitrogen concentration and pH change diagram in a certain period during the culture of nitrifying bacteria in the production process of the high-load full-process nitrifying bacteria agent in the specific embodiment.

[0046] Figure 3 It is an ammonia nitrogen concentration and pH change diagram in a certain period during the culture of nitrifying bacteria in the production process of the high-load full-process nitrifying bacteria agent in the specific embodiment. Figure 2 It is an ammonia nitrogen concentration and pH change diagram in a certain period during the culture of nitrifying bacteria in the production process of the high-load full-process nitrifying bacteria agent in the specific embodiment.

[0047] Figure 4 It is an ammonia nitrogen concentration and pH change diagram in a certain period during the culture of nitrifying bacteria in the production process of the high-load full-process nitrifying bacteria agent in the specific embodiment. DETAILED DESCRIPTION

[0048] The present application will be further described below in combination with the drawings and specific embodiments. It should be understood that these embodiments are only used to illustrate the present application and are not used to limit the scope of the present application. The operation methods not specified in the following embodiments are usually performed according to the conventional conditions or the conditions suggested by the manufacturers.

[0049] Reference should be made to Figure 1The application discloses a production device of high-load full-range nitrification bacterial agent, which comprises a fermentation expansion tank body 1, a first air compressor 15-1 and a second air compressor 15-3. The fermentation expansion tank body 1 is a polyethylene cylindrical barrel with a volume of 500 L (diameter 84 cm, height 100 cm) and a top cover. The fermentation expansion tank body 1 is internally provided with a first heating rod 2-1, a first temperature sensor 2-2, a pH sensor 16, an ammonia nitrogen sensor 17, a filler frame 6 and a microporous aeration disc 18. The first heating rod 2-1, the first temperature sensor 2-2, the second air compressor 15-3, the pH sensor 16 and the ammonia nitrogen sensor 17 are connected with a PLC control system 11 through a cable 7, and the PLC control system 11 is provided with a pH display screen 9 and an ammonia nitrogen concentration display screen 10. The filler frame 6 is internally added with uncoated hydrophilic polyurethane porous filler and hydrophilic polyurethane porous filler loaded with activated AOB and NOB, wherein the specific surface area of the uncoated hydrophilic polyurethane porous filler is 300-800 m 2 / m 3The cover of the fermentation expansion tank 1 can be opened, which is convenient for taking out the filler frame 6 and replacing new filler. The filler frame 6 is a stainless steel mesh cylindrical frame body, the outer diameter of which is 80 cm, slightly smaller than the inner diameter of the fermentation expansion tank 1, and the height is 65 cm, which is used to fix the filler. The fermentation expansion tank 1 is provided with an upper drainage port 3-1 and a lower drainage port 3-2 on the side; the upper drainage port 3-1 is connected to the drainage pipe 4-2 through the drainage pump 4-1, and the drainage pipe 4-2 is directly connected to the waste liquid cylinder or the sewer pipe, which can realize the discharge of the supernatant of the culture medium in the fermentation expansion tank 1, and the distance between the upper drainage port 3-1 and the lower boundary of the fermentation expansion tank 1 is 75 cm; the lower drainage port 3-2 is close to the bottom of the fermentation expansion tank 1, which can be used for drainage and also can be used for quickly collecting culture solution and suspended bacteria, and the distance between the lower drainage port 3-2 and the lower boundary of the fermentation expansion tank 1 is 10 cm. The top of the fermentation expansion tank 1 is provided with a liquid supplementing port 5-1. The upper side of the liquid supplementing port 5-1 is connected to the sodium carbonate liquid supplementing barrel 12, the sodium bicarbonate liquid supplementing barrel 13 and the ammonium chloride liquid supplementing barrel 14 through the sodium carbonate liquid supplementing pump 8-1, the sodium bicarbonate liquid supplementing pump 8-2 and the ammonium chloride liquid supplementing pump 8-3 respectively, and the sodium carbonate liquid supplementing pump 8-1, the sodium bicarbonate liquid supplementing pump 8-2 and the ammonium chloride liquid supplementing pump 8-3 are all peristaltic pumps connected to the PLC control system 11 and form interlocking with the pH sensor 16 and the ammonia nitrogen sensor 17 under the control of the PLC control system 11. The sodium carbonate liquid supplementing barrel 12 is provided with a second heating rod 12-1 and a second temperature sensor 12-2, and the second heating rod 12-1 and the second temperature sensor 12-2 are connected to the PLC control system 11 through the cable 7. The lower side of the liquid supplementing port 5-1 is connected to the gas-liquid distribution pipe 5-2, the outer diameter of which is 9 cm, the gas-liquid distribution pipe 5-2 extends into the filler frame 6 and corresponds to the side of the filler frame 6, and the gas-liquid distribution pipe 5-2 is provided with jagged holes with a diameter of 0.5-1 cm. The first air compressor 15-1 is connected to the air inlet pipe 15-2, the air inlet pipe 15-2 penetrates into the gas-liquid distribution pipe 5-2 from the bottom surface of the gas-liquid distribution pipe 5-2 and is sealingly connected to the bottom surface of the gas-liquid distribution pipe 5-2, and the air inlet pipe 15-2 in the gas-liquid distribution pipe 5-2 is provided with holes on the side. The microporous aeration disc 18 is located below the filler frame 6 in the bottom of the fermentation expansion tank 1, has a diameter of 75 cm, is connected to the second air compressor 15-3, and the second air compressor 15-3 is connected to the PLC control system 11 and forms interlocking with the ammonia nitrogen sensor 17 under the control of the PLC control system 11, that is, the PLC control system 11 can control the working condition of the second air compressor 15-3 according to the detection result of the ammonia nitrogen sensor 17.

[0050] The preparation process of the hydrophilic polyurethane porous filler loaded with activated AOB and NOB includes the following steps: 1) inoculate AOB into the activated culture medium at an inoculation amount of 5 g / L, and add the specific surface area of 300-800 m 2 / m 3The hydrophilic polyurethane porous filler is cultured in the medium for 24 hours at 30℃ with a rotation speed of 100 rpm, and the pH value of the activated medium is 7.8, and the components of the activated medium include 0.2 g / L NH4Cl, 0.5 g / L K2HPO4, 0.1 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 0.3 g / L CaCO3 and 1 g / L Na2CO3. 2) After the ammonia nitrogen conversion rate in the medium in step 1) reaches 30%, NOB is added in a mass ratio of 1.5:1 to AOB, and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB is obtained by continuing to culture for 48 hours at a rotation speed of 150 rpm and a temperature of 30℃, which meets the requirement of dissolved oxygen for the growth of NOB.

[0051] A production process of a high-load full-nitrification bacterial agent, which uses the production device of the high-load full-nitrification bacterial agent shown in Figure 1 as a threshold upper limit to a threshold lower limit as a batch cycle (i.e., a domestication stage), and uses a multi-cycle and multi-batch enrichment culture method, and specifically includes the following steps: The expansion culture medium is added into the fermentation expansion tank 1, and the first heating rod 2-1 is controlled to work according to the detection result of the first temperature sensor 2-2 by the PLC control system 11, so that the temperature in the fermentation expansion tank 1 is controlled at 30℃; the pH value of the expansion culture medium is 7.5, and the components include 0.2 g / L NH4Cl, 0.625 g / L NaHCO3, 0.05 g / L Na2CO3, 0.078 g / L NaH2PO4, 0.1 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 0.03 g / L CaCl2·2H2O and 1 mL / L trace element concentrate; the pH value of the trace element concentrate is 6.5, and the components include 0.5 g / L FeCl2·4H2O, 0.011 g / L MnCl2·4H2O, 0.07 g / L ZnCl2, 0.006 g / L H3BO3, 0.036 g / L Na2MoO4·2H2O, 0.014 g / L NiCl2·6H2O and 0.002 g / L CuCl2·2H2O; The volume ratio of the uncoated hydrophilic polyurethane porous filler and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB is 1:1, and the total volume of the uncoated hydrophilic polyurethane porous filler and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB added in the filler frame 6 to the total volume of the culture medium in the fermentation expansion tank body 1 is 40:100. The dissolved oxygen concentration in the fermentation expansion tank body 1 is controlled by adjusting the aeration pressure of the first air compressor 15-1 and / or the second air compressor 15-3. The first air compressor 15-1 is turned on, and the second air compressor 15-3 is turned on if necessary (for example, when the ammonia nitrogen sensor 17 detects that the ammonia nitrogen concentration is >200 mg / L, the PLC control system 11 controls the second air compressor 15-3 to be turned on, and the microporous aeration disc 18 is aerated), so that the dissolved oxygen concentration of the culture medium in the fermentation expansion tank body 1 is 3-6 mg / L. The step-by-step domestication of ammonia nitrogen concentration is carried out, and the minimum threshold and the maximum threshold of the ammonia nitrogen sensor 17 are set for each domestication stage. The minimum threshold and the maximum threshold of the pH sensor 16 are also set during the step-by-step domestication process. The PLC control system 11 controls the operation of the sodium bicarbonate solution pump 8-2 and the ammonium chloride solution pump 8-3 according to the triggering of the ammonia nitrogen sensor 17, and then controls the sodium bicarbonate and ammonium chloride solution, and controls the operation of the sodium carbonate solution pump 8-1 according to the triggering of the pH sensor 16, and then controls the sodium carbonate solution, so that the ammonia nitrogen and pH of the culture medium in the fermentation expansion tank body 1 are within the set threshold range during the domestication process.

[0052] For example, when the pH of the culture medium decreases to the minimum threshold of the pH sensor 16, the PLC control system 11 controls the sodium carbonate solution pump 8-1 to be turned on to add sodium carbonate, and when the pH of the culture medium increases to the maximum threshold of the pH sensor 16, the PLC control system 11 controls the sodium carbonate solution pump 8-1 to be turned off to stop adding sodium carbonate, so as to realize automatic control of the pH of the culture medium and ensure that the pH of the culture medium is always within the set threshold range. The above-mentioned pH control process is throughout the entire step-by-step domestication process, and only one minimum threshold 7.8 of the pH sensor 16 and one maximum threshold 8.8 of the pH sensor 16 are set during the entire step-by-step domestication process.

[0053] The control logic of the ammonia nitrogen concentration in the culture medium can be similar to the control logic of the pH of the culture medium. For example, when the ammonia nitrogen concentration in the culture medium decreases to the lower threshold value triggered by the ammonia nitrogen sensor 17, the PLC control system 11 controls the sodium bicarbonate solution pump 8-2 and the ammonium chloride solution pump 8-3 to open to supplement sodium bicarbonate and ammonium chloride until the ammonia nitrogen concentration in the culture medium increases to the upper threshold value triggered by the ammonia nitrogen sensor 17, at which time the PLC control system 11 controls the sodium bicarbonate solution pump 8-2 and the ammonium chloride solution pump 8-3 to close to stop the sodium bicarbonate and ammonium chloride supplementation, thereby achieving automatic control of the ammonia nitrogen concentration in the culture medium and ensuring that the ammonia nitrogen concentration in the culture medium is always within the set threshold range.

[0054] In the stepwise acclimation process, the ammonia nitrogen concentration is increased by 50 mg / L per step. The lower threshold value triggered by the ammonia nitrogen sensor 17 in each acclimation stage is 50 mg / L. The upper threshold value triggered by the ammonia nitrogen sensor 17 in each acclimation stage is selected from 100 to 300 mg / L, specifically: the upper threshold value triggered by the ammonia nitrogen sensor 17 in the first acclimation stage is 100 mg / L, the upper threshold value triggered by the ammonia nitrogen sensor 17 in the second acclimation stage is 150 mg / L, the upper threshold value triggered by the ammonia nitrogen sensor 17 in the third acclimation stage is 200 mg / L, the upper threshold value triggered by the ammonia nitrogen sensor 17 in the fourth acclimation stage is 250 mg / L, and the upper threshold value triggered by the ammonia nitrogen sensor 17 in the fifth acclimation stage is 300 mg / L. From the fifth acclimation stage, the upper threshold value triggered by the ammonia nitrogen sensor 17 in each subsequent acclimation stage is 300 mg / L. In any acclimation stage, when the ammonia nitrogen concentration decreases to the lower threshold value triggered by the ammonia nitrogen sensor 17, the PLC control system 11 controls the sodium bicarbonate solution pump 8-2 and the ammonium chloride solution pump 8-3 to open to supplement sodium bicarbonate and ammonium chloride until the ammonia nitrogen concentration increases to the upper threshold value triggered by the ammonia nitrogen sensor 17 in the next acclimation stage, at which time the PLC control system 11 controls the sodium bicarbonate solution pump 8-2 and the ammonium chloride solution pump 8-3 to close to stop the sodium bicarbonate and ammonium chloride supplementation and enter the next acclimation stage. The amount of ammonium chloride and sodium bicarbonate added is controlled and adjusted by the flow rate of the ammonium chloride solution pump 8-3 and the sodium bicarbonate solution pump 8-2, and the ratio will significantly affect the ammonia nitrogen degradation rate and the accumulation of nitrite nitrogen. For example, when sodium bicarbonate and ammonium chloride are supplemented, the ratio of the volume of sodium bicarbonate solution added to the volume of ammonium chloride solution added is 2.5, the mass concentration of the ammonium chloride solution is 32%, and the mass concentration of the sodium bicarbonate solution is 9%.

[0055] During the stepwise acclimation process, the culture medium is discharged through the upper drain 3-1 to prevent the culture medium from overflowing in the fermentation expansion tank. For example, after the upper drain 3-1 is opened, the drain pump 4-1 is opened, and the culture medium is discharged through the drain pipe 4-2. For example, the aeration can be stopped for 3 hours every 20 hours of operation, and after the suspended bacteria are fully settled, the drain is opened to discharge the supernatant. Each operation-stop aeration-drainage is referred to as a cycle. Several batches of water samples are selected for each cycle to investigate the ammonia oxidation rate AOR and the nitrite accumulation rate NAR at different culture stages in the same cycle; the batch is just added with ammonia nitrogen at t0, and the reaction is sampled at t1 to measure NH4 + -N, NO2 - -N, NO3 - -N, and the concentration is denoted as [NH4 + -N]A0, [NO2 - -N]A0, [NO3 - -N]A0, [NH4 + -N]A1, [NO2 - -N]A1, [NO3 - -N]A1; at the same time, 10 pieces of filler are randomly selected for each cycle, washed with sterile water, and the bacterial mass concentration ρ is measured to calculate the ammonia oxidation rate AOR of the cycle. Figure 2 The figure for the change of ammonia nitrogen concentration and pH in a cycle during the cultivation of nitrifying bacteria. Figure 3 The figure for the change of ammonia nitrogen concentration and pH in a cycle during the cultivation of nitrifying bacteria. Figure 2 The figure for the ammonia oxidation rate and the concentrations of nitrite nitrogen and nitrate nitrogen in a certain acclimation stage in the cycle. Figure 4 The figure for the ammonia oxidation rate in different culture stages in a cycle during the cultivation of nitrifying bacteria. The ammonia oxidation rate AOR (unit: mg NH4 + -N / (g·h) refers to the amount of ammonia nitrogen that can be oxidized by unit mass concentration of bacteria (unit: g / L) per unit time, and the formula is as follows: AOR=([NH4 + -N]A1-[NH4 + -N]A0) / [(t1-t0)·ρ] NOR=([NO2 - -N]A1-[NO2 - -N]A0) / [(t1-t0)·ρ] The production process of the above high-load full-nitrification bacterial agent, the nitrite nitrogen accumulation in the stepwise acclimation process is not more than 30 mg / L. During the stepwise acclimation process, the ammonia oxidation rate AOR≥400 mg NH4 +When the concentration of nitrate nitrogen in the fermentation expansion tank is higher than 12000 mg / L, the production of high-load full nitrification bacteria agent is completed, aeration is stopped, and the bacteria are collected after 4 hours of sedimentation, thereby obtaining the expanded microbial agent, i.e., the high-load full nitrification bacteria agent. Of course, only part of the bacteria can be collected, for example, 50%, and the remaining bacteria can be cultured again from the beginning of the step-by-step acclimation process, and the ammonia nitrogen concentration acclimation and carbon source compensation are performed again until a new batch of bacteria agent reaches the harvesting standard.

[0056] Furthermore, it is understood that various modifications and changes can be made to the application by those skilled in the art in light of the above description of the present application, and such equivalent forms are to be included in the scope of the claims appended hereto.

Claims

1. A production apparatus for high-load, full-process nitrifying bacteria agent, characterized in that, The high-load full-nitrification bacteria agent production device comprises a fermentation expansion tank and a first air compressor. The fermentation expansion tank is provided with a first heating rod, a first temperature sensor, a pH sensor, an ammonia nitrogen sensor and a filler frame. The fermentation expansion tank is provided with an upper water outlet and a lower water outlet on the side surface, and the lower water outlet is close to the bottom of the fermentation expansion tank. The top end of the fermentation expansion tank is provided with a liquid supplementing port.

2. The apparatus for producing high-load total nitrification bacteria agent according to claim 1, wherein The first air compressor is connected with an air inlet pipe. The high-load full-nitrification bacteria agent production device further comprises a second air compressor.

3. The apparatus for producing high-load total nitrification bacteria agent according to claim 1, wherein The specific surface area of the uncoated hydrophilic polyurethane porous filler is 300-800 m 2 / m 3 ; The second air compressor and the microporous aeration disc are used as a supplementary aeration equipment to ensure sufficient dissolved oxygen when the ammonia nitrogen concentration in the fermentation expansion tank is greater than 200 mg / L.

4. The apparatus for producing high-load total nitrification bacteria agent according to claim 1, wherein The side surface of the gas-liquid distribution pipe corresponding to the filler frame is provided with a sawtooth-shaped sieve hole with a pore size of 0.5-2 cm. The preparation process of the hydrophilic polyurethane porous filler loaded with activated AOB and NOB comprises the following steps: 1) inoculate AOB into an activation medium at an inoculation amount of 2-10 g / L, add hydrophilic polyurethane porous filler, and shake culture; the pH value of the activation medium is 7.7-7.9, and the components include 0.1-0.3 g / L NH4Cl, 0.3-0.5 g / L K2HPO4, 0.1 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 0.1-0.3 g / L CaCO3 and 1-2 g / L Na2CO3; 5. The apparatus for producing high-load total nitrification bacteria agent according to claim 1, wherein 2) after the ammonia nitrogen conversion rate in the medium of step 1) reaches 30%-50%, add NOB at a mass ratio of NOB:AOB of 1-2:1, continue to shake culture for 24-48 h, and the rotation speed of the shake culture meets the requirement of dissolved oxygen for NOB growth, to obtain the hydrophilic polyurethane porous filler loaded with activated AOB and NOB. The sodium carbonate liquid supplementing barrel is provided with a second heating rod and a second temperature sensor.

6. The high-load full-nitrification bacteria agent production device according to any one of claims 1-5 is used in the production of high-load full-nitrification bacteria agent.

7. A process for the production of high load total nitrification inoculants, characterized by, The production device of the high-load full nitrification bacterial agent according to any one of claims 1-5; The production process of the high-load full nitrification bacterial agent comprises: The fermentation expansion tank is added with an expansion culture medium, and a PLC control system is used to control the working condition of the first heating rod according to the detection result of the first temperature sensor, so as to control the temperature in the fermentation expansion tank at 30±2℃; the pH value of the expansion culture medium is 7.4-7.6, and the components include: 0.08-0.20 g / L NH4Cl, 0.425-0.625 g / L NaHCO3, 0.03-0.05 g / L Na2CO3, 0.078 g / L NaH2PO4, 0.1 g / L MgSO4·7H2O, 0.01 g / L FeSO4·7H2O, 0.03 g / L CaCl2·2H2O, and 1 mL / L trace element concentrate; the pH value of the trace element concentrate is 6.0-6.5, and the components include: 0.5 g / L FeCl2·4H2O, 0.011 g / L MnCl2·4H2O, 0.07 g / L ZnCl2, 0.006 g / L H3BO3, 0.036 g / L Na2MoO4·2H2O, 0.014 g / L NiCl2·6H2O, and 0.002 g / L CuCl2·2H2O; The uncoated hydrophilic polyurethane porous filler and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB are added into the filler frame; The first air compressor is started to make the dissolved oxygen concentration of the culture medium in the fermentation expansion tank be 3-6 mg / L; The stepwise domestication with gradual increase of ammonia nitrogen concentration is carried out, and the ammonia nitrogen sensor is triggered to set the minimum threshold value and the maximum threshold value in each domestication stage, and the pH sensor is triggered to set the minimum threshold value and the maximum threshold value in the stepwise domestication process; the PLC control system controls the operation of the sodium bicarbonate solution pump and the ammonium chloride solution pump according to the triggering condition of the ammonia nitrogen sensor, and then controls the solution of sodium bicarbonate and ammonium chloride, controls the operation of the sodium carbonate solution pump according to the triggering condition of the pH sensor, and then controls the solution of sodium carbonate, so that the ammonia nitrogen and pH of the culture medium in the fermentation expansion tank in the domestication process are within the set threshold value range.

8. The production process of high-load total nitrification bacteria agent according to claim 7, characterized by, The ratio of the total volume of the uncoated hydrophilic polyurethane porous filler and the hydrophilic polyurethane porous filler loaded with activated AOB and NOB added into the filler frame to the total volume of the culture medium in the fermentation expansion tank is 30-50:100; The volume ratio of the uncoated hydrophilic polyurethane porous filler to the hydrophilic polyurethane porous filler loaded with activated AOB and NOB added into the filler frame is 1-3:

1.

9. The production process of high-load total nitrification bacteria agent according to claim 7, characterized by, In the stepwise domestication process, the culture medium is regularly / irregularly discharged through the upper water outlet to prevent the culture medium in the fermentation expansion tank from overflowing; In the stepwise domestication process, the increase of ammonia nitrogen concentration is 10-50 mg / L per level; The minimum threshold value of the ammonia nitrogen sensor in each domestication stage is selected from 20-120 mg / L; The minimum threshold value of the ammonia nitrogen sensor in each domestication stage is selected from 20-120 mg / L; The ammonia nitrogen sensor trigger maximum threshold value of each acclimation stage is selected from 100-350 mg / L, and since the second acclimation stage, the ammonia nitrogen sensor trigger maximum threshold value of any acclimation stage is higher than or equal to the ammonia nitrogen sensor trigger maximum threshold value of the previous acclimation stage; In any acclimation stage, when the ammonia nitrogen concentration decreases to the ammonia nitrogen sensor trigger minimum threshold value, the PLC control system controls the sodium bicarbonate and ammonium chloride liquid supplement pumps to open for sodium bicarbonate and ammonium chloride liquid supplement, until the ammonia nitrogen concentration increases to the ammonia nitrogen sensor trigger maximum threshold value of the next acclimation stage, at which time the PLC control system controls the sodium bicarbonate and ammonium chloride liquid supplement pumps to close to stop sodium bicarbonate and ammonium chloride liquid supplement and enter the next acclimation stage; The pH sensor trigger minimum threshold value is selected from 7.2-7.8; The pH sensor trigger maximum threshold value is selected from 8.0-8.

8.

10. The production process of high-load total nitrification bacteria agent according to claim 7, characterized by, The nitrite nitrogen accumulation amount in the stepwise acclimation process is not more than 30 mg / L; In the stepwise acclimation process, the ammonia oxidation rate AOR≥400 mg NH4 + When the nitrate nitrogen concentration in the fermentation expansion tank is higher than 12000 mg / L, the production of high-load full nitrification bacteria is completed.

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