Complex microbial inoculant for tobacco as well as production device and production method of complex microbial inoculant

Abstract

The invention discloses a complex microbial inoculant for tobacco as well as a production device and a production method of the complex microbial inoculant. The complex microbial inoculant comprises the following materials: 2 parts of bacillus amyloliquefaciens, 2 parts of bacillus mucilaginosus, 4 parts of bacillus megatherium and 2 parts of dibutyl phthalate degrading bacteria. A liquid seed culture medium (g / L): 10 g of NaCl, 10 g of peptone and 10 g of yeast powder; the fermentation medium (g / L) comprises 50 g of corn starch, 70 g of soybean meal, 3 g of peptone, 1 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 0.2 g of ferrous sulfate, 0.02 g of manganese sulfate and 5 g of light calcium carbonate; bacillus amyloliquefaciens, bacillus megaterium, bacillus mucilaginosus and dibutyl phthalate degrading bacteria are selected to prepare the multifunctional composite microbial agent, and the prepared composite microbial agent has multiple functions of antagonizing pathogenic bacteria, promoting nutrient absorption, degrading allelopathic autotoxic substances and the like. The multifunctional complex microbial inoculant and a macromolecular bioactive substance polyglutamic acid are synergistically applied, so that the activity of the strain is enhanced, and a synergistic interaction effect is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tobacco planting, in particular to a compound microbial agent for tobacco and a production device and method thereof. BACKGROUND

[0002] Due to the limitations of factors such as arable land resources, planting conditions and production costs, the current tobacco planting mode often adopts the mode of perennial continuous cropping, and the phenomenon of unreasonable application of chemical fertilizers is also common, which causes the deterioration of tobacco planting soil, the imbalance of beneficial functional flora, the aggravation of disease and pest damage, the hindering of tobacco growth and development, and further affects the yield of tobacco, especially the improvement of tobacco quality.

[0003] At present, in the agricultural production of tobacco, beneficial microorganisms play an important role in promoting the growth and development of tobacco and preventing diseases, and certain practical applications have been achieved, but the common microbial agents often have insufficient composition and function, and the strain adaptability is not strong, which is difficult to achieve the ideal effect of tobacco disease resistance and growth promotion. Especially for the phenomenon of long-term continuous cropping of tobacco, a large amount of allelopathic autotoxic substances will accumulate in the tobacco planting soil, which seriously restricts the growth and development level of tobacco itself. SUMMARY

[0004] The purpose of the present application is to provide a compound microbial agent for tobacco and a production device and method thereof to solve the problems raised in the background.

[0005] To achieve the above purpose, the present application provides the following technical scheme: a compound microbial agent for tobacco, comprising the following materials: 2 parts of Bacillus amyloliquefaciens, 2 parts of Bacillus mucilaginosus, 4 parts of Bacillus megaterium and 2 parts of dibutyl phthalate-degrading bacteria; Liquid seed culture medium (g / L): NaCl 10, peptone 10 and yeast powder 10; Fermentation medium (g / L): corn starch 50, soybean meal 70, peptone 3, potassium dihydrogen phosphate 1, magnesium sulfate 0.5, ferrous sulfate 0.2, manganese sulfate 0.02 and light calcium 5.

[0006] A fermentation device for a compound microbial agent for tobacco, comprising a fermentation tank and a tank cover, an inner tooth ring is fixedly installed in the inside of the fermentation tank, a liquid outlet pipe is communicated with the bottom of the fermentation tank, a driving motor is fixedly installed on the top of the tank cover, a stirring assembly is installed on the output end of the driving motor, an additive pipe is communicated with the top of the tank cover, and a liquid injection pipe is communicated with the top of the tank cover.

[0007] Preferably, the stirring assembly comprises a main stirring assembly and an auxiliary stirring assembly, the main stirring assembly comprises a main stirring rod, the top of the main stirring rod is fixedly connected with the output end of the driving motor, the outer side of the main stirring rod is fixedly installed with a plurality of main stirring blades, the bottom of the main stirring rod is fixedly installed with a connecting rod, and the bottom of the connecting rod is fixedly installed with an arc-shaped stirring rod.

[0008] Preferably, the auxiliary stirring assembly comprises two connecting strips and two auxiliary stirring rods, one end of each of the two connecting strips is fixedly connected with the outer side of the main stirring rod, the inner side of the end of each of the two connecting strips away from the main stirring rod is rotatably installed with a rotating column, the bottom of each of the rotating columns is fixedly installed with a gear, the bottom of each of the gears is fixedly installed with a fixed column, the outer side of each of the fixed columns is rotatably sleevedly installed with the main stirring rod, the bottom of each of the connecting strips is fixedly installed with a sliding slot frame, the top of each of the auxiliary stirring rods is fixedly installed with a sliding block, the outer side of each of the auxiliary stirring rods is fixedly installed with an auxiliary stirring frame, and the outer side of each of the auxiliary stirring rods is fixedly installed with an auxiliary stirring blade.

[0009] Preferably, the outer side of the gear is engaged with the inner side of the inner tooth ring, the sliding block is slidably installed in the inner side of the sliding slot frame, and the end of the main stirring rod away from the fixed column is rotatably sleevedly installed on the outer side of the auxiliary stirring rod.

[0010] Preferably, the outer side of the auxiliary stirring rod is fixedly installed with a limiting ring, and the top of the limiting ring is in contact with the bottom of the main stirring rod.

[0011] Preferably, the outer side of the auxiliary stirring rod is fixedly installed with an auxiliary stirring strip, and the auxiliary stirring strip and the auxiliary stirring frame are arranged in a spaced manner.

[0012] Preferably, the top of the fermentation tank is fixedly installed with a flange plate two, the bottom of the tank cover is fixedly installed with a flange plate one, and the flange plate one and the flange plate two are sealingly connected through bolts.

[0013] Preferably, the outer side of the fermentation tank is symmetrically fixedly installed with four support connecting blocks, the bottom of each of the four support connecting blocks is fixedly installed with a support leg, and the outer side of the liquid outlet pipe is fixedly installed with an electromagnetic valve.

[0014] A fermentation preparation method of a composite microbial agent for tobacco: S1: Four kinds of fermentation bacteria seed liquid preparation: Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria are respectively activated by using eggplant bottle solid culture medium, and are respectively transferred to 50mL liquid seed culture medium, and are cultured at 37℃ by using a shaking bed at 230rpm, and after 12h, the above four liquid seed liquids are respectively secondarily inoculated into 250mL liquid seed culture medium at a inoculation ratio of 2%, and are cultured at 37℃ by using a shaking bed at 230rpm for 12h.

[0015] S2: Fermenter treatment: clean and empty the internal fermenter for sterilization, configure the fermentation medium according to 50% liquid volume, and add the medium into the interior of the fermenter through the liquid injection pipe, and again implement empty sterilization, then add the seed liquid of four fermentation strains of Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria into the interior of the fermenter through the additive pipe in sequence.

[0016] S3: Preparation of solid multifunctional composite microbial agent: the four fermentation strain seed liquids are inoculated in the ratio of Bacillus amyloliquefaciens: Bacillus mucilaginosus: Bacillus megaterium: dibutyl phthalate degrading bacteria 2:2:4:2, the inoculation amount is 3%, after 36h fermentation, multifunctional composite microbial fermentation liquid is obtained, then the electromagnetic valve is controlled to open, the multifunctional composite microbial fermentation liquid is discharged into the container through the liquid outlet pipe, and the multifunctional composite microbial fermentation liquid is subjected to spray drying treatment to obtain a solid multifunctional composite microbial agent.

[0017] Compared with the prior art, the beneficial effects of the present application are: by selecting Bacillus amyloliquefaciens, Bacillus megaterium, Bacillus mucilaginosus and dibutyl phthalate degrading bacteria, the multifunctional composite microbial agent prepared by the multifunctional composite microbial agent has the functions of pathogenic bacteria antagonism, nutrient absorption promotion and degradation of allelopathic and autotoxic substances, etc., and when applied in tobacco fields, the multifunctional composite microbial agent is synergistically applied with macromolecular bioactive substance polyglutamic acid, which is a water-soluble, biodegradable, non-toxic biological macromolecule prepared by microbial fermentation method, can provide a carrier for the growth of functional strains, enhance the activity of the strains, and achieve a synergistic effect. In addition, the main stirring assembly and the auxiliary stirring assembly synergistically stir, the material mixing coefficient in the fermenter can be increased to more than 0.9, effectively avoiding the problems of too high or too low local concentration of the strains, ensuring uniform distribution of the number of strains in each milliliter of fermentation liquid, providing a stable environment for the metabolic synergy of the four strains, the carbohydrates decomposed by Bacillus amyloliquefaciens can provide carbon source for other strains, the sticky substances secreted by Bacillus mucilaginosus can enhance the cohesiveness between strains, and the stress-resistant environment created by Bacillus megaterium can enhance the activity of the target degrading bacteria, finally the degradation rate of dibutyl phthalate is increased by 20%-30%, and the fermentation period is shortened by 15%-20%, significantly improving the fermentation efficiency and functional effect. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a three-dimensional structure schematic diagram of the fermentation device of the present application.

[0019] Figure 2 It is another perspective three-dimensional structure schematic diagram of the fermentation device of the present application.

[0020] Figure 3The figure is a three-dimensional structure diagram of the fermenting tank of the present application.

[0021] Figure 4 The figure is a three-dimensional structure diagram of the tank cover and stirring assembly of the present application.

[0022] Figure 5 The figure is a three-dimensional structure diagram of the fermenting tank of the present application. Figure 4 The figure is an enlarged structure diagram of A in the present application.

[0023] Figure 6 The figure is a three-dimensional structure diagram of the tank cover and stirring assembly of the present application from another perspective.

[0024] In the figure: 1, fermenting tank; 2, tank cover; 3, driving motor; 4, liquid injection pipe; 5, additive injection pipe; 6, flange plate 1; 7, flange plate 2; 8, support connecting block; 9, support leg; 10, electromagnetic valve; 11, liquid outlet pipe; 12, connecting rod; 13, inner tooth ring; 14, arc-shaped stirring rod; 15, auxiliary stirring blade; 16, auxiliary stirring rod; 17, auxiliary stirring frame; 18, auxiliary stirring strip; 19, main stirring blade; 20, gear; 21, main stirring rod; 22, rotating column; 23, connecting strip; 24, pushing strip; 25, limiting ring; 26, fixed column; 27, sliding slot frame; 28, sliding block. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work are within the protection scope of the present application.

[0026] Please refer to Figures 1-6The application provides a technical scheme: a composite microbial inoculant for tobacco, which comprises the following materials: 2 parts of Bacillus amyloliquefaciens, 2 parts of Bacillus mucilaginosus, 4 parts of Bacillus megaterium and 2 parts of dibutyl phthalate-degrading bacteria, the composite microbial inoculant needs to simultaneously realize antagonism against pathogenic bacteria, promote nutrient absorption and degrade dibutyl phthalate, the dibutyl phthalate-degrading bacteria directly acts on dibutyl phthalate accumulated in soil, eliminates the inhibition of dibutyl phthalate on tobacco root growth and relieves continuous cropping obstacles, which is a key link of multifunctionality of the composite microbial inoculant, the Bacillus amyloliquefaciens, the Bacillus mucilaginosus and the Bacillus megaterium mainly undertake the functions of secreting plant hormones and inhibiting bacteria, and the dibutyl phthalate-degrading bacteria can improve the colonization efficiency of other bacteria by removing dibutyl phthalate, so that the inhibition of self-toxic substances on the activity of functional bacteria is avoided, the species of the dibutyl phthalate-degrading bacteria mainly include Pseudomonas, Bacillus and Acinetobacter, the characteristics of strains between different species have certain differences, in actual production, the dibutyl phthalate-degrading bacteria can be selected according to the actual use and demand of the composite microbial inoculant, when the specific dibutyl phthalate-degrading bacteria is selected, the specific dibutyl phthalate-degrading bacteria can be determined according to the actual application scene and environmental conditions, if efficient degradation of dibutyl phthalate is needed and the environmental conditions are suitable, Pseudomonas can be selected; if degradation under various environmental conditions is needed, Bacillus can be selected; in addition, according to environmental adaptability and safety, when the microbial inoculant is used for tobacco soil, Bacillus is preferentially selected as the dibutyl phthalate-degrading bacteria, the Bacillus has the characteristics of high temperature resistance and acid and alkali resistance, has strong compatibility with the survival conditions of other Bacillus in the composite microbial inoculant and meets the safety standards of agricultural microbial inoculants; The liquid seed culture medium is NaCl 10 g / L, protein peptone 10 g / L and yeast powder 10 g / L, and the liquid seed culture medium provides nutrients required by the Bacillus amyloliquefaciens, the Bacillus mucilaginosus, the Bacillus megaterium and the dibutyl phthalate-degrading bacteria during culture, so that the Bacillus amyloliquefaciens, the Bacillus mucilaginosus, the Bacillus megaterium and the dibutyl phthalate-degrading bacteria generate corresponding liquid seed solutions; The fermentation culture medium is corn starch 50 g / L, soybean meal 70 g / L, protein peptone 3 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate 0.5 g / L, ferrous sulfate 0.2 g / L, manganese sulfate 0.02 g / L and light calcium 5 g / L, and the fermentation culture medium provides nutrients required by the Bacillus amyloliquefaciens, the Bacillus mucilaginosus, the Bacillus megaterium and the dibutyl phthalate-degrading bacteria during fermentation, so that the fermentation effect of the Bacillus amyloliquefaciens, the Bacillus mucilaginosus, the Bacillus megaterium and the dibutyl phthalate-degrading bacteria is ensured, thereby ensuring that the multifunctional composite microbial fermentation liquid can be stably generated.

[0027] A kind of fermentation device for the compound microbial inoculant of tobacco, including fermentation tank 1 and tank cover 2, the inside fixed mounting of fermentation tank 1 has internal gear ring 13, the bottom of fermentation tank 1 is communicated with liquid outlet pipe 11, the top of tank cover 2 is fixedly installed with drive motor 3, the output end of drive motor 3 is installed with stirring assembly, stirring assembly includes main stirring assembly and auxiliary stirring assembly, main stirring assembly includes main stirring rod 21, the top of main stirring rod 21 is fixedly connected with the output end of drive motor 3, the outside of main stirring rod 21 is fixedly installed with multiple main stirring piece 19, the bottom of main stirring rod 21 is fixedly installed with connecting rod 12, the bottom of connecting rod 12 is fixedly installed with arc-shaped stirring rod 14, the top of tank cover 2 is communicated with additive pipe 5, the top of tank cover 2 is communicated with liquid injection pipe 4, auxiliary stirring assembly includes two connecting strips 23 and two auxiliary stirring rods 16, one end of two connecting strips 23 is fixedly connected with the outside of main stirring rod 21, the inside of the end of two connecting strips 23 away from main stirring rod 21 is rotatably installed with rotating column 22, the bottom of rotating column 22 is fixedly installed with gear 20, the bottom of gear 20 is fixedly installed with fixed column 26, the outside of fixed column 26 is rotatably sleeved with main stirring rod 21, the bottom of connecting strip 23 is fixedly installed with sliding slot frame 27, the top of auxiliary stirring rod 16 is fixedly installed with sliding block 28, the outside of auxiliary stirring rod 16 is fixedly installed with auxiliary stirring frame 17, the outside of auxiliary stirring rod 16 is fixedly installed with auxiliary stirring piece 15, the outside of gear 20 and the inside of internal gear ring 13 are engaged, sliding block 28 is slidably installed in the inside of sliding slot frame 27, the end of main stirring rod 21 away from fixed column 26 is rotatably sleeved with the outside of auxiliary stirring rod 16, auxiliary stirring rod 16 is fixedly installed with auxiliary stirring strip 18 on the outside, and auxiliary stirring strip 18 and auxiliary stirring frame 17 are arranged at intervals.

[0028] The culture medium can be added to the inside of the fermentation tank 1 through the liquid injection pipe 4. During the liquid injection process, the volume of the culture medium needs to be monitored in real time through the liquid level sensor installed inside the fermentation tank 1 to ensure that it reaches the preset fermentation level, which is usually 60%-70% of the tank capacity, to avoid material overflow during subsequent stirring due to too high liquid level, or to avoid affecting the metabolic environment of the strain due to too low liquid level. After the liquid injection is completed, the valve outside the liquid injection pipe 4 is closed and the liquid injection pipe 4 is cleaned in situ to prevent residual culture medium from breeding bacteria. Then the Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria seed liquids are added to the inside of the fermentation tank 1 through the additive pipe 5 in turn. The Bacillus amyloliquefaciens seed liquid is injected first because it has strong carbohydrate decomposition ability and can provide basic metabolic products for the system in advance. After standing for 10 minutes, the Bacillus mucilaginosus seed liquid is injected. This bacterium can secrete sticky substances and needs to be preliminarily mixed with the former to avoid local agglomeration. After standing for another 15 minutes, the Bacillus megaterium seed liquid is injected. This bacterium has strong stress resistance and can quickly colonize in the mixed system. Finally, the dibutyl phthalate degrading bacteria seed liquid is injected. As the target functional bacteria, it needs to achieve efficient degradation in the metabolic environment constructed by other bacteria. During the addition process, the positive pressure state in the additive pipe 5 needs to be maintained, and the pressure is controlled at 0.2-0.3MPa. After the addition is completed, an external sterile filter is added inside the additive pipe 5 for filtration to prevent external bacteria from being contaminated. Then the driving motor 3 is started. The output end of the driving motor 3 can drive the main stirring rod 21 to rotate, thereby driving the main stirring blade 19, the connecting rod 12 and the arc-shaped stirring rod 14 to rotate, realizing stirring of the material in the inside of the fermentation tank 1, thereby fully rotating and stirring the fermentation medium and the seed liquids of Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria in the inside of the fermentation tank 1, thereby ensuring the fermentation effect and improving the fermentation efficiency. In addition, when the main stirring rod 21 rotates, it can drive the connecting strip 23 to rotate, and in cooperation with the internal tooth ring 13, it can make the gear 20 rotate, thereby making the fixed column 26 rotate, thereby making the main stirring rod 21 move, pushing the auxiliary stirring rod 16 to move, making the sliding block 28 move in the inside of the sliding slot frame 27, thereby making the auxiliary stirring rod 16 reciprocate along the connecting strip 23, thereby making the auxiliary stirring strip 18 and the auxiliary stirring frame 17 reciprocate, fully horizontally stirring and mixing the fermentation medium and the seed liquids of Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria in the inside of the fermentation tank 1, thereby improving the stirring and mixing efficiency and being helpful for better fermentation and improving the fermentation effect. The auxiliary stirring strip 18 is in the shape of a rhombus net frame and can disperse large-volume material groups into small particles, thereby further improving the mixing uniformity. Through the stirring synergy of the main stirring assembly and the auxiliary stirring, the material mixing coefficient in the fermentation tank 1 can be improved to 0.9 The above effectively avoids the problem of excessive or insufficient local concentration of bacteria, ensuring uniform distribution of bacteria in each milliliter of fermentation broth, providing a stable environment for the metabolic synergy of the four bacteria, and the carbohydrates decomposed by Bacillus amyloliquefaciens can provide carbon sources for other bacteria, the sticky substances secreted by Bacillus mucilaginosus can enhance the cohesiveness between bacteria, and the stress-resistant environment created by Bacillus megaterium can enhance the activity of the target degrading bacteria, ultimately increasing the degradation rate of dibutyl phthalate by 20%-30%, while shortening the fermentation period by 15%-20%, significantly improving the fermentation efficiency and functional effect.

[0029] In another embodiment, as shown in Figures 1-6 The outer side of the auxiliary stirring rod 16 is fixedly installed with a limiting ring 25, and the top of the limiting ring 25 is in contact with the bottom of the main stirring rod 21.

[0030] The limiting ring 25 can limit the position of the main stirring rod 21.

[0031] In another embodiment, as shown in Figures 1-6 The top of the fermentation tank 1 is fixedly installed with a flange plate two 7, the bottom of the tank cover 2 is fixedly installed with a flange plate one 6, the flange plate one 6 and the flange plate two 7 are sealingly connected through bolts, the outer side of the fermentation tank 1 is symmetrically fixedly installed with four support connecting blocks 8, the bottom of each of the four support connecting blocks 8 is fixedly installed with a support leg 9, and the outer side of the liquid outlet pipe 11 is fixedly installed with an electromagnetic valve 10.

[0032] The flange plate one 6 and the flange plate two 7 can be used to sealingly assemble the fermentation tank 1 and the tank cover 2, facilitating disassembly and assembly of the fermentation tank 1 and the tank cover 2, thereby facilitating subsequent maintenance and repair of the devices inside the fermentation tank 1 and the tank cover 2, including but not limited to cleaning of the gear 20 and the inner tooth ring 13.

[0033] A fermentation preparation method of a composite microbial agent for tobacco: S1: Four kinds of fermentation bacteria seed liquid preparation: Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria are activated using eggplant bottles solid culture medium, and are respectively transferred to 50mL liquid seed culture medium, and are cultured at 37℃ with a shaker at 230rpm, and after 12h, the above four liquid seed culture media are respectively inoculated into 250mL liquid seed culture medium at a inoculation ratio of 2%, and are cultured at 37℃ with a shaker at 230rpm for 12h.

[0034] S2: Fermenter treatment: clean and empty the inner fermenter 1 to sterilize, configure the fermentation medium according to 50% liquid volume, and add the medium into the inner fermenter 1 through the liquid injection pipe 4, and sterilize again, then add the seed liquid of Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria into the inner fermenter 1 through the additive pipe 5.

[0035] S3: Preparation of solid multifunctional composite microbial agent: the seed liquid of the four kinds of fermentation bacteria is inoculated according to Bacillus amyloliquefaciens: Bacillus mucilaginosus: Bacillus megaterium: dibutyl phthalate degrading bacteria 2:2:4:2, the inoculation amount is 3%, and the multifunctional composite microbial fermentation liquid is obtained after 36h fermentation, then the electromagnetic valve 10 is controlled to open, the multifunctional composite microbial fermentation liquid is discharged into the container through the liquid outlet pipe 11, and the multifunctional composite microbial fermentation liquid is subjected to spray drying treatment to obtain the solid multifunctional composite microbial agent.

[0036] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A complex microbial inoculant for tobacco, characterized in that: Comprise the following materials: 2 parts of Bacillus amyloliquefaciens, 2 parts of Bacillus mucilaginosus, 4 parts of Bacillus megaterium and 2 parts of dibutyl phthalate-degrading bacteria Liquid seed culture medium (g / L): NaCl 10, protein peptone 10 and yeast powder 10; Fermentation medium (g / L): corn starch 50, soybean meal 70, protein peptone 3, potassium dihydrogen phosphate 1, magnesium sulfate 0.5, ferrous sulfate 0.2, manganese sulfate 0.02 and light calcium 5.

2. A fermentation device for a complex microbial inoculum for tobacco, applied to a complex microbial inoculum for tobacco according to claim 1, characterized in that: It comprises a fermentation tank (1) and a tank cover (2), the inside of the fermentation tank (1) is fixedly installed with an internal gear ring (13), the bottom of the fermentation tank (1) is communicated with a liquid outlet pipe (11), the top of the tank cover (2) is fixedly installed with a driving motor (3), the output end of the driving motor (3) is installed with a stirring assembly, the top of the tank cover (2) is communicated with an additive pipe (5), and the top of the tank cover (2) is communicated with a liquid injection pipe (4).

3. The fermentation device for the complex microbial agent of tobacco according to claim 2, characterized in that: The stirring assembly comprises a main stirring assembly and an auxiliary stirring assembly, the main stirring assembly comprises a main stirring rod (21), the top of the main stirring rod (21) is fixedly connected with the output end of the driving motor (3), a plurality of main stirring blades (19) are fixedly installed on the outer side of the main stirring rod (21), and the bottom of the main stirring rod (21) is fixedly installed with a connecting rod (12).

4. The fermentation device for the complex microbial agent of tobacco according to claim 3, characterized in that: The bottom of the connecting rod (12) is fixedly installed with an arc-shaped stirring rod (14).

5. The fermentation device for the complex microbial agent of tobacco according to claim 4, characterized in that: The auxiliary stirring assembly comprises two connecting strips (23) and two auxiliary stirring rods (16), one end of each of the two connecting strips (23) is fixedly connected with the outer side of the main stirring rod (21), the inside of the end of each of the two connecting strips (23) away from the main stirring rod (21) is rotatably installed with a rotating column (22), the bottom of the rotating column (22) is fixedly installed with a gear (20), the bottom of the gear (20) is fixedly installed with a fixed column (26), the outer side of the fixed column (26) is rotatably sleeved with the main stirring rod (21), the bottom of the connecting strip (23) is fixedly installed with a sliding groove frame (27), the top of the auxiliary stirring rod (16) is fixedly installed with a sliding block (28), the outer side of the auxiliary stirring rod (16) is fixedly installed with an auxiliary stirring frame (17), and the outer side of the auxiliary stirring rod (16) is fixedly installed with an auxiliary stirring blade (15).

6. The fermentation device for the complex microbial agent of tobacco according to claim 5, characterized in that: The outer side of the gear (20) is engaged with the inner side of the internal gear ring (13), the sliding block (28) is slidably installed in the inside of the sliding groove frame (27), and the end of the main stirring rod (21) away from the fixed column (26) is rotatably sleeved with the outer side of the auxiliary stirring rod (16).

7. The fermentation device for the complex microbial agent of tobacco according to claim 6, characterized in that: The outer side of the auxiliary stirring rod (16) is fixedly installed with a limiting ring (25), and the top of the limiting ring (25) is in contact with the bottom of the main stirring rod (21). The outer side of the auxiliary stirring rod (16) is fixedly installed with an auxiliary stirring strip (18), and the auxiliary stirring strip (18) and the auxiliary stirring frame (17) are arranged in a spaced manner.

8. The fermentation device for the complex microbial agent of tobacco according to claim 7, characterized in that: The top of the fermentation tank (1) is fixedly installed with a flange plate two (7), the bottom of the tank cover (2) is fixedly installed with a flange plate one (6), and the flange plate one (6) and the flange plate two (7) are connected by bolt sealing.

9. The fermentation device for the complex microbial agent of tobacco according to claim 8, characterized in that: The outer side of the fermentation tank (1) is symmetrically fixedly installed with four support connecting blocks (8), the bottom of each of the four support connecting blocks (8) is fixedly installed with a support leg (9), and the outer side of the liquid outlet pipe (11) is fixedly installed with a solenoid valve (10).

10. A fermentation preparation method of a complex microbial inoculum for tobacco, applied to the complex microbial inoculum for tobacco according to claim 1 and the fermentation device of a complex microbial inoculum for tobacco according to claims 2-9, characterized in that: The method comprises the following steps: S1: four kinds of fermentation seed liquid preparation: Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria are respectively activated by using eggplant bottle solid culture medium, and are respectively transferred to 50mL liquid seed culture medium, and are cultured at 37℃ by using a shaking bed at 230rpm, and after 12h, the above four liquid seed liquids are respectively secondarily inoculated into 250mL liquid seed culture medium at a inoculation ratio of 2%, and are cultured at 37℃ by using a shaking bed at 230rpm for 12h; S2: fermentation tank treatment: clean and sterilize the internal fermentation tank (1) by air sterilization, configure fermentation medium according to 50% liquid loading, and add the medium into the internal fermentation tank (1) through the liquid injection pipe (4), and sterilize again by air sterilization, then sequentially add the four kinds of fermentation seed liquids of Bacillus amyloliquefaciens, Bacillus mucilaginosus, Bacillus megaterium and dibutyl phthalate degrading bacteria into the internal fermentation tank (1) through the additive pipe (5); S3: preparation of solid multifunctional compound microbial agent: the four kinds of fermentation seed liquids are inoculated at a ratio of Bacillus amyloliquefaciens: Bacillus mucilaginosus: Bacillus megaterium: dibutyl phthalate degrading bacteria 2:2:4:2, the inoculation amount is 3%, and after fermentation for 36h, multifunctional compound microbial fermentation liquid is obtained, then the solenoid valve (10) is opened, the multifunctional compound microbial fermentation liquid is discharged into a container through the liquid outlet pipe (11), and the multifunctional compound microbial fermentation liquid is subjected to spray drying treatment to obtain a solid multifunctional compound microbial agent.

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