EDTA (Ethylene Diamine Tetraacetic Acid) decalcification device with multi-stage filtering and circulating functions

Abstract

The invention relates to an EDTA (Ethylene Diamine Tetraacetic Acid) decalcification device with multi-stage filtering and circulating functions. The EDTA decalcification device comprises a dissolving frame and a lower contact assembly, the first electric push rod is fixedly connected to the top of the dissolving frame, the lower contact assembly comprises a second vacuum pump, and the second vacuum pump is connected to the bottom of the dissolving frame through bolts. The reagent is prepared from sodium hydroxide, disodium ethylene diamine tetraacetate dihydrate, deionized water, sodium hydroxide and a Tris-HCl buffer solution. A bone focus with active metabolism is recognized through PET-CT scanning, bone needle biopsy is conducted under the guidance of B-mode ultrasound / CT, a bone tissue sample is obtained, and the sample can be moved into the decalcification device to be dissolved. According to the EDTA decalcification device with the multi-stage filtering and circulating functions, the cavity forms a negative pressure state, the contact needle is in contact with the surface of a bone sheet, and the bone sheet is adsorbed through negative pressure difference, so that external decalcification liquid enters the flow guide cavity through pores of the bone sheet, the decalcification liquid is effectively driven and penetrates through the whole bone sheet, and the decalcification effect is improved. Liquid transmission from one end to the other end is realized, and the decalcification time is shortened.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tissue decalcification technology, in particular to an EDTA decalcification device with multi-stage filtering and circulating functions. BACKGROUND

[0002] The patent with publication (announcement) No. CN218308032U discloses a device for accelerating the decalcification rate of EDTA decalcification solution, which comprises a device body, a decalcification bottle and a support, the device body comprises a base, the base is provided with a mounting bracket on one side, a lifting cylinder is arranged in the mounting bracket, the piston rod of the lifting cylinder is connected with a cross bar, a guide limiting column is arranged on the upper part of the mounting bracket, one side of the cross bar is inserted into the guide limiting column, the cross bar slides up and down along the guide limiting column under the drive of the lifting cylinder, and a stirring motor is arranged at the bottom of the other end of the cross bar.

[0003] In the above-mentioned prior art, the support is placed in the decalcification bottle, is placed in the cup groove, the decalcification solution is added, the upper placing plate is covered, then the heating cup pad is turned on and heated to a constant temperature, the lifting cylinder is controlled to drive the stirring paddle to descend to the space between the lower placing plate and the upper placing plate, the stirring motor is turned on to start stirring, the decalcification is accelerated, and only the single storage effect of the bone pieces is achieved in the decalcification bottle, and the decalcification acceleration and the detection of the decalcification degree of the bone pieces during the decalcification process cannot be achieved, so that the decalcification efficiency of the bone pieces is low.

[0004] Therefore, the EDTA decalcification device with multi-stage filtering and circulating functions is proposed to solve the problems in the above-mentioned prior art. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application provides an EDTA decalcification device with multi-stage filtering and circulating functions, which can solve the above-mentioned problems.

[0006] To achieve the above-mentioned purposes, the present application provides the following technical scheme: a dissolving frame and a lower contact assembly are arranged, the inside of the dissolving frame is hollow for filling the decalcification solution; A first electric push rod is fixedly connected to the top of the dissolving frame, and the telescopic end of the first electric push rod is fixedly connected with a sliding plate through the side wall of the top of the dissolving frame; the lower contact assembly is fixedly connected with the side wall of the limiting frame; The lower contact assembly comprises a second vacuum pump, which is bolted to the bottom of the dissolving frame; The side wall of the limiting frame is fixedly connected with a lower containing frame, the inside of the lower containing frame is hollow to form a cavity, and the bottom of the cavity is connected with one end of the second vacuum pump through a connecting pipe; The side wall of the lower containing frame is slidably connected with a sliding frame, the inside of the sliding frame is hollow, and a rubber film is connected to the edge of the hollow part of the sliding frame, so as to block and close the hollow part of the sliding frame to form a supplementary cavity; The effect achieved by the above-mentioned component is that the periodic pressure is applied to the sealed space wrapped with the bone piece through the expansion and contraction of the rubber film, so as to promote the exchange of fresh liquid and waste liquid inside the bone piece. A plurality of stress assembly groups are connected to the top of the lower accommodating frame, and a stress rod is fixedly connected to the bottom of the sliding frame and is hollowed in the stress rod and communicates with the supplement cavity. The effect achieved by the above-mentioned component is that the single-point or multi-point position softening detection is performed on the bone piece by the stress assembly group, and the single-point or multi-point position adjustable suction strength is performed on the bone piece.

[0007] Preferably, a limiting frame is fixedly connected to the inner bottom of the dissolving frame, and a sliding plate is slidingly connected to the limiting frame. An upper contact assembly is fixedly connected to the side wall of the sliding plate. The upper contact assembly is provided with a first telescopic pipe and a second telescopic pipe at the top, and the second telescopic pipe is connected to the first vacuum pump at the top. The effect achieved by the above-mentioned component is that the first vacuum pump is used to form negative pressure in the hollow cavity inside the upper contact assembly.

[0008] Preferably, the stress rod communicates with one end of an air pump, and the side wall of the air pump is fixedly connected to the telescopic end of a second electric push rod. The effect achieved by the above-mentioned component is that the second electric push rod provides power for the movement of the stress rod, and the stress rod simultaneously provides power for the height of the sliding frame. The second electric push rod is fixedly connected to the side wall of the dissolving frame. The stress rod is slidingly connected to the inner side wall of the dissolving frame, and a pressure relief valve is embedded in the lower end of the side wall of the stress rod.

[0009] Preferably, the stress assembly group includes a contact needle, and the contact needle is fixedly connected to the bottom of the inner cylinder. The effect achieved by the above-mentioned component is that the upper and lower ends of the bone piece are in point contact with the top of the plurality of contact needles, so as to reduce the contact area of the bone piece and the contact needle, thereby increasing the contact area of the bone piece and the decalcification solution. The inner cylinder is slidingly connected to the outer cylinder, and a distance sensor is bolted to the top of the outer cylinder. The effect achieved by the above-mentioned component is that when the contact needle is inserted into the bone piece, if the bone piece is not completely decalcified, resistance will be generated, so that the contact needle moves upward to drive the inner cylinder to slide into the outer cylinder, and the distance between the top of the inner cylinder and the distance sensor inside the outer cylinder is detected, so as to automatically identify the degree of decalcification of the bone piece.

[0010] The side wall of the contact needle is fixedly connected to one end of a return spring, the other end of the return spring is fixedly connected to a guide cylinder, and the contact needle is slidingly connected to the guide cylinder.

[0011] Preferably, the guide cylinder is connected to one end of a three-way electromagnetic valve, and the other end of the three-way electromagnetic valve is connected to a first matching pipe. The other end of the three-way electromagnetic valve is connected with the second matching pipe; The first matching pipe and the second matching pipe are both provided with a one-way valve, and the upper ends of the first matching pipe and the second matching pipe are in communication with the cavity.

[0012] Preferably, the contact needle is provided with a flow guide cavity penetrating through the contact needle, and the flow guide cavity is in communication with the guide cylinder; The upper contact assembly comprises an upper containing frame, and the side wall of the containing frame is fixedly connected with an upper embedding frame; and the upper containing frame is fixedly connected with the sliding plate.

[0013] Preferably, the upper containing frame is composed of a lower containing frame, a cavity and a stress assembly.

[0014] Preferably, the first telescopic pipe and the second telescopic pipe are in communication with the cavity in the upper containing frame.

[0015] Preferably, the dissolving frame is provided with a valve at the bottom, and the valve is in communication with the upper end of the filter box. A plurality of filters are arranged in the filter box, and the conveying pipe on the side wall of the filter box is in communication with the suction pump. The above components achieve the effect that waste liquid can be automatically discharged periodically through the valve, the filter box and the suction pump, and the waste liquid can be recycled or recovered after being filtered.

[0016] Compared with the prior art, the EDTA decalcification device with multi-stage filtering and circulating functions has the following beneficial effects: 1. The cavity forms a negative pressure state, the contact needle is in contact with the surface of the bone piece, and the bone piece is adsorbed through the negative pressure difference, so that the external decalcification liquid enters the flow guide cavity inside through the pores of the bone piece, effectively drives the decalcification liquid to penetrate through the entire bone piece, realizes liquid transmission from one end to the other end, and shortens the decalcification time; The negative pressure intensity in the cavity is controlled to adjust the suction intensity of the contact needle to the bone piece, and the flow rate of the decalcification liquid is controlled. Through the expansion and contraction of the rubber film, periodic pressure is applied to the sealed space wrapping the bone piece, which can further promote the exchange of fresh liquid and waste liquid inside the bone piece.

[0017] 2. The contact of the contact needle and the bone piece is arranged in multiple points, which facilitates the simultaneous multi-point detection of the upper and lower ends of the bone piece; when the contact needle is inserted into the bone piece, if the bone piece is not completely decalcified, resistance will be generated, so that the contact needle moves upward to drive the inner cylinder to slide and insert into the outer cylinder, and the distance between the distance sensor inside the outer cylinder and the top of the inner cylinder is detected, so as to automatically identify the decalcification degree of the bone piece.

[0018] 3. The upper and lower ends of the bone pieces make point contact with the top of the multiple groups of contact needles, reducing the contact area of the bone pieces and the contact needles and increasing the contact area of the liquid and the bone pieces; by controlling the operation of a single group of force receiving assemblies, the decalcification liquid can be accelerated in a single area of the bone pieces, or multiple groups of force receiving assemblies can operate to suck the whole area of the bone pieces. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 is a schematic diagram of the three-dimensional structure of the present application Figure 1 ; Figure 2 is a schematic diagram of the three-dimensional structure of the present application Figure 2 Figure 3 is a schematic diagram of the three-dimensional structure of the present application Figure 3 ; Figure 4 is a schematic diagram of the three-dimensional structure of the present application Figure 4 ; Figure 5 is a schematic diagram of the connection structure of the lower contact assembly and the limiting frame of the present application Figure 6 is a schematic diagram of the structure of the upper contact assembly of the present application Figure 7 is a schematic diagram of the structure of the force receiving assembly of the present application Figure 1 ; Figure 8 is a schematic diagram of the structure of the force receiving assembly of the present application Figure 2 .

[0020] In the figure: 1, dissolution frame; 2, first electric push rod; 3, limiting frame; 4, sliding plate; 5, upper contact assembly; 6, first telescopic pipe; 7, second telescopic pipe; 8, first vacuum pump; 9, lower contact assembly; 10, valve; 11, filter box; 12, filter; 13, suction pump; 91, second vacuum pump; 92, conveying pipe; 93, lower containing frame; 94, cavity; 95, sliding frame; 96, supplementary cavity; 97, rubber film; 98, force receiving assembly; 99, force receiving rod; 910, air pump; 911, second electric push rod; 981, contact needle; 982, inner cylinder; 983, outer cylinder; 984, guide cylinder; 985, return spring; 986, three-way electromagnetic valve; 987, first matching pipe; 988, second matching pipe; 9811, flow guide cavity; 51, upper containing frame; 52, upper embedded frame. DETAILED DESCRIPTION

[0021] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0022] Embodiment: Please refer to Figure 1 - Figure 8 The EDTA decalcification device with multi-stage filtering and circulating functions in the embodiment comprises a dissolving frame 1, the inside of the dissolving frame 1 is hollowed out for filling decalcification solution; a door leaf is hingedly connected to the front end of the dissolving frame 1, and a transparent glass observation window is arranged on the door leaf; the first electric push rod 2 is fixedly connected to the top of the dissolving frame 1, and the telescopic end of the first electric push rod 2 is fixedly connected with the sliding plate 4 through the top sidewall of the dissolving frame 1. The controller is electrically connected with the electrical components. Specifically, the inside sidewall of the dissolving frame 1 is provided with a temperature sensor and a semiconductor refrigerating fin, the hot end of the semiconductor refrigerating fin is located at the outer end of the dissolving frame 1, the cold end is located in the inside of the dissolving frame 1 and contacts with the decalcification solution, the heat is transferred to reduce the temperature, and the outer end of the dissolving frame 1 is provided with a cooling fan for discharging the heat generated by the hot end of the semiconductor refrigerating fin outward. Specifically, the bottom of the limiting frame 3 is fixedly connected to the bottom of the dissolving frame 1, and the sliding plate 4 is slidingly connected in the limiting frame 3; the upper contact assembly 5 is fixedly connected to the sidewall of the sliding plate 4. The first electric push rod 2 drives the sliding plate 4 to move, the sliding plate 4 drives the upper contact assembly 5 to move up and down, and the upper contact assembly 5 can push the flow of the decalcification solution in the process of moving up and down, so that the upper contact assembly 5 contacts or separates from the top of the bone piece, which is convenient for subsequent contact, extrusion and transfer of the bone piece.

[0023] In the embodiment, please refer to Figure 1 - Figure 5 The top of the upper contact assembly 5 is respectively provided with the first telescopic pipe 6 and the second telescopic pipe 7, the first telescopic pipe 6 can be connected with the pipeline for conveying the decalcification solution outside, the first telescopic pipe 6 and the second telescopic pipe 7 can be telescopic or unfolded with the upper contact assembly 5, and the top of the second telescopic pipe 7 is connected with the first vacuum pump 8; the first vacuum pump 8 is used for forming negative pressure in the hollow cavity 94 of the upper contact assembly 5. The lower contact assembly 9 is fixedly connected with the sidewall of the limiting frame 3; the lower contact assembly 9 comprises a second vacuum pump 91 which is bolted to the bottom of the dissolving frame 1. The lower containing frame 93 is fixedly connected with a side wall of the limiting frame 3, and a cavity 94 is formed in the lower containing frame 93, and the bottom of the cavity 94 is connected with one end of the second vacuum pump 91 through a connecting pipe, and a one-way valve is arranged in the connecting pipe; Specifically, the lower containing frame 93 and the bottom of the upper containing frame 51 are each provided with a liquid discharge valve which is in communication with the cavity 94, so that the liquid in the cavity 94 can be easily discharged. In actual use, the second vacuum pump 91 forms negative pressure in the cavity 94, and the cavity 94 extracts air in the front end region of the contact needle 981 through the negative pressure difference, and because the contact needle 981 is in contact with the bone piece and extracts liquid in the bone piece through the negative pressure, the flow of the decalcification solution in the bone piece is accelerated, and the exchange of the decalcification solution and the substances in the bone piece is accelerated.

[0024] In this embodiment, please refer to Figure 1 , Figure 4 The side wall of the lower containing frame 93 is slidably connected with a sliding frame 95, the sliding frame 95 is hollow, and the edge of the hollow part of the sliding frame 95 is connected with a rubber film 97, and the rubber film 97 blocks and closes the hollow part of the sliding frame 95 to form a supplementary cavity 96. The sliding frame 95 can stir the decalcification solution during the up-down movement of the sliding frame 95, and the decalcification solution is driven to flow. In actual use, the supplementary cavity 96 is filled with air, so that the gas in the supplementary cavity 96 increases, and the gas drives the rubber film 97 to expand as a whole. The top of the lower containing frame 93 is connected with a plurality of stress components 98, the stress components 98 are used to contact the bone piece, the bottom of the sliding frame 95 is fixedly connected with a stress rod 99, the stress rod 99 is hollow and in communication with the supplementary cavity 96, and an external air pump 910 can extract external air into the inside of the supplementary cavity 96. The stress rod 99 is in communication with one end of the air pump 910, the side wall of the air pump 910 is fixedly connected with the telescopic end of a second electric push rod 911, and the second electric push rod 911 is fixedly connected with the side wall of the dissolving frame 1. The stress rod 99 is slidably connected with the inner side wall of the dissolving frame 1, the stress rod 99 is used to drive the sliding frame 95 to slide and adjust the position, and a pressure relief valve is embedded in the lower end of the side wall of the stress rod 99, so that the gas in the supplementary cavity 96 can be easily discharged.

[0025] In this embodiment, please refer to Figure 7 , Figure 8 , Figure 1 and Figure 5 The stress component 98 comprises a contact needle 981, the contact needle 981 is fixedly connected with the bottom of an inner cylinder 982, the inner cylinder 982 is slidably connected with an outer cylinder 983, and a distance sensor is bolted to the top of the outer cylinder 983. The side wall of the contact needle 981 is fixedly connected with one end of the reset spring 985, the other end of the reset spring 985 is fixedly connected with the guide cylinder 984, and the contact needle 981 is slidably connected with the guide cylinder 984; The guide cylinder 984 is connected with one end of the three-way electromagnetic valve 986, the other end of the three-way electromagnetic valve 986 is connected with the first matching pipe 987, and the other end of the three-way electromagnetic valve 986 is connected with the second matching pipe 988; The first matching pipe 987 and the second matching pipe 988 are each provided with a one-way valve, and the upper ends of the first matching pipe 987 and the second matching pipe 988 are in communication with the cavity 94. The contact needle 981 is provided with a flow guide cavity 9811 penetratingly arranged therein, and the flow guide cavity 9811 is in communication with the guide cylinder 984.

[0026] In this embodiment, please refer to ​ - ​ The upper contact assembly 5 includes an upper containing frame 51, and the side wall of the containing frame 51 is fixedly connected with an upper embedded frame 52, and the upper containing frame 51 is fixedly connected with the sliding plate 4. The upper containing frame 51 is composed of a lower containing frame 93, a cavity 94 and a stress assembly 98. The first telescopic pipe 6 and the second telescopic pipe 7 are in communication with the cavity 94 in the upper containing frame 51. Specifically, the dissolving frame 1 is provided with a valve 10 at the bottom, the valve 10 is in communication with the upper end of the filter box 11, a plurality of groups of filters 12 are arranged in the filter box 11, a conveying pipe on the side wall of the filter box 11 is in communication with a suction pump 13, the other end of the suction pump 13 is connected with a recycling device outside, and the suction pump 13 transmits the filtered liquid to the recycling device inside so as to be recycled. The first group of filters 12 is specifically a filter membrane with a pore size of 50-100 μm, which removes physical debris generated in the decalcification process, such as bone powder, bone marrow, fiber debris, etc. The second group of filters 12 is provided with chelating resin, which has a very high affinity for calcium ions, can adsorb and fix Ca²⁺ in EDTA-Ca in the liquid on the resin, and at the same time releases free and active EDTA molecules back into the solution. The third group of filters 12 is specifically a microporous filter membrane filter, which has a thin film with precise and uniform small pore size, and the filter membrane is commonly made of polyether sulfone, which is biologically inert and does not react with EDTA decalcification solution.

[0027] The specific composition of the decalcification solution is: ethylenediaminetetraacetic acid disodium dihydrate: deionized water / distilled water: sodium hydroxide: Tris-HCl buffer solution: The preparation method of the decalcification solution is as follows: EDTA disodium is added to about 800 mL of deionized water, and magnetic stirring is performed; Slowly add sodium hydroxide particles while continuously stirring and monitoring the pH, EDTA will completely dissolve when the pH is close to 8.0; After EDTA is completely dissolved, add Tris base.

[0028] Adjust the pH to 7.2-7.4 with HCl (such as 10N HCl) or NaOH (such as 10N NaOH) solution; Dilute the solution to 1L and store at room temperature; a small amount of crystals may precipitate, which can be redissolved by heating or short-term microwave heating.

[0029] Among them, the decalcification time is specifically; Small / thin animal tissues (such as mouse femur head, <2mm): 2-5 days are required.

[0030] Routine biopsy or small piece of tissue (3-5mm): 1-3 weeks are required.

[0031] Large animal or large mass of human bone (>5mm): 4 weeks to several months are required.

[0032] Specifically, through PET-CT scanning, metabolically active bone lesions are identified, and bone puncture biopsy is performed under the guidance of B-ultrasound / CT, and bone tissue samples are obtained and dissolved in a movable decalcification device.

[0033] The working principle of the above embodiment is: First, when decalcifying the bone piece, the bone piece is placed on the top of the plurality of contact needles 981, the bone piece is supported by the contact needles 981, and then the decalcification solution is filled and placed in the inside of the dissolution frame 1, so that the decalcification solution contacts the bone piece; The first electric push rod 2 drives the upper contact assembly 5 to move downward, and the contact needles 981 at the bottom of the upper contact assembly 5 contact the upper end of the bone piece, so that the upper and lower ends of the bone piece are in point contact with the top of the plurality of contact needles 981, thereby reducing the contact area of the bone piece and the contact needles 981 and increasing the contact area of the liquid and the bone piece; Second, control the second electric push rod 911 to drive the stress rod 99 to move upward, and the stress rod 99 drives the sliding frame 95 to move upward, so that the sliding frame 95 is embedded in the inside of the upper embedding frame 52, so that the sliding frame 95 wraps the gap between the upper embedding frame 52 and the side wall of the lower containing frame 93, and the bone piece is in a sealed space; Turn on the air pump 910 to extract air into the stress rod 99, and then deliver it to the supplement cavity 96 to make the rubber film 97 swell, and the rubber film 97 swells to improve the sealing performance of the space between the upper embedding frame 52 and the lower containing frame 93 where the bone piece is placed; Subsequently, the rubber film 97 continues to expand and press the decalcification solution in the sealed space, increasing the pressure in the sealed space. The pressure relief valve is then controlled to allow the gas in the supplement cavity 96 to be discharged outward, and the rubber film 97 is expanded or reduced in a cycle; Third, the second vacuum pump 91 is controlled to extract air from the cavity 94 in the lower containing frame 93, so that the cavity 94 forms a negative pressure state. Then, the three-way electromagnetic valve 986 on the force assembly 98 is opened, the second matching pipe 988 on the three-way electromagnetic valve 986 is communicated with the cavity 94, the cavity 94 is communicated with the flow guide cavity 9811 on the contact needle 981, and the contact needle 981 is in contact with the surface of the bone piece and absorbs the bone piece through the negative pressure difference. The decalcification solution outside the bone piece enters the flow guide cavity 9811 through the pores of the bone piece, effectively driving the decalcification solution through the entire bone piece, achieving liquid transmission from one end to the other end, and shortening the decalcification time; The negative pressure intensity in the cavity 94 is controlled to adjust the suction intensity of the contact needle 981 on the bone piece and control the flow rate of the decalcification liquid; By controlling the operation of a single set of force assemblies 98, the decalcification liquid can be accelerated in a single area of the bone piece, or multiple sets of force assemblies 98 can be operated to suck the entire area of the bone piece; Fourth, when it is necessary to replace the decalcification solution in the dissolving frame 1, the valve 10 and the suction pump 13 are opened, the liquid is filtered through the filter box 11, and the suction pump 13 is delivered to the external equipment; The three-way electromagnetic valve 986 on the force assembly 98 is connected to the first matching pipe 987, one end is opened, the decalcification solution outside is delivered to the delivery pipe 92, then the delivery pipe 92 delivers the liquid to the inside of the cavity 94, and then the first matching pipe 987 in the cavity 94 enters the flow guide cavity 9811, and finally the contact needle 981 head region delivers the liquid to the outer end of the bone piece. When the new decalcification solution is delivered, the upper and lower ends of the bone piece are pre-cleaned by the contact needles 981 in contact with the upper and lower ends of the bone piece, and the old residual decalcification solution is cleaned and falls to the lower end. The decalcification solution after cleaning falls to the valve 10 and is delivered outward, avoiding the mixing of the old decalcification solution with the new decalcification solution, and then the new decalcification solution is delivered to the flow guide cavity 9811 and then transmitted to the contact needle 981 region in contact with the bone piece, so that the decalcification solution is pre-washed and contacted with the surface of the bone piece when the decalcification solution is replaced; Fifth, and after a long time of decalcification, the first electric push rod 2 drives the sliding plate 4 to slide down, the sliding plate 4 drives the upper contact assembly 5 to contact the bone piece, the contact pins 981 at the upper and lower ends are extruded with the bone piece, the contact pins 981 are extruded with the bone piece under force, when the bone piece is decalcified, the contact pins 981 can be inserted into the bone piece without resistance, and when the bone piece is not completely decalcified, the contact pins 981 have resistance when inserted into the bone piece, the contact pins 981 move upwards to drive the inner cylinder 982 to slide and insert into the outer cylinder 983, and the distance between the top of the inner cylinder 982 and the distance sensor in the inner cylinder 983 is detected, and the degree of decalcification of the bone piece is identified, and because the contact of the contact pins 981 with the bone piece is multi-point arranged and facilitates multi-point detection of the upper and lower ends of the bone piece.

[0034] The mounting mode, connection mode or arrangement mode disclosed in the embodiment are all common mechanical connection modes, and can be implemented as long as the beneficial effects can be achieved, so the specific structural components and working principles will not be described in detail.

[0035] It should be noted that, in this document, the terms such as first and second are used merely to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or device. Without more limitations, the element defined by the statement "including a" does not exclude the presence of another identical element in the process, method, article or device including the element.

[0036] Although the embodiments of the present application have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and alterations can be made thereto without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An EDTA decalcification device with multi-stage filtration and circulation functions, comprising a dissolution frame (1) and a lower contact assembly (9), wherein the interior of the dissolution frame (1) is hollowed out for filling with decalcification solution; The first electric push rod (2) is fixedly connected to the top of the dissolving frame (1), and the telescopic end of the first electric push rod (2) passes through the top side wall of the dissolving frame (1) and is fixedly connected to the sliding plate (4). Its features are: The lower contact component (9) is fixedly connected to the side wall of the limiting frame (3); The lower contact assembly (9) includes a second vacuum pump (91), which is bolted to the bottom of the melting frame (1); The side wall of the limiting frame (3) is fixedly connected to the lower receiving frame (93), and the lower receiving frame (93) is hollowed out to form a cavity (94). The bottom of the cavity (94) is connected to one end of the second vacuum pump (91) through a connecting pipe. A sliding frame (95) is slidably connected to the side wall of the lower receiving frame (93). The sliding frame (95) is hollow inside, and a rubber membrane (97) is connected to the edge of the hollow part of the sliding frame (95). The rubber membrane (97) blocks and seals the hollow part inside the sliding frame (95) to form a supplementary cavity (96). The expansion and contraction of the rubber membrane (97) applies periodic pressure to the sealed space enclosing the bone fragments, promoting the exchange of fresh liquid and waste liquid inside the bone fragments. The top of the lower receiving frame (93) is connected to multiple sets of force-bearing components (98), and the bottom of the sliding frame (95) is fixedly connected to a force-bearing rod (99). The force-bearing rod (99) is hollow and communicates with the supplementary cavity (96). The bone fragments are subjected to single-point or multi-point softening detection and single-point or multi-point adjustable suction intensity by force-bearing component (98).

2. The EDTA decalcification device with multi-stage filtration and circulation functions according to claim 1, characterized in that: The limiting frame (3) is fixedly connected to the bottom of the dissolving frame (1), and a sliding plate (4) is slidably connected inside the limiting frame (3). Upper contact assembly (5) is fixedly connected to the side wall of sliding plate (4); The top of the upper contact assembly (5) is provided with a first telescopic tube (6) and a second telescopic tube (7), and the top of the second telescopic tube (7) is connected to the first vacuum pump (8).

3. An EDTA decalcification device with multi-stage filtration and circulation functions according to claim 1 or 2, characterized in that: The force-bearing rod (99) is connected to one end of the air pump (910), and the side wall of the air pump (910) is fixedly connected to the telescopic end of the second electric push rod (911); The second electric push rod (911) is fixedly connected to the side wall of the melting frame (1); The force-bearing rod (99) is slidably connected to the inner wall of the dissolving frame (1), and a pressure relief valve is embedded in the lower end of the side wall of the force-bearing rod (99).

4. An EDTA decalcification device with multi-stage filtration and circulation functions according to claim 1 or 2, characterized in that: The force-bearing component (98) includes a contact pin (981), which is fixedly connected to the bottom of the inner cylinder (982); The inner cylinder (982) is slidably connected to the outer cylinder (983), and a distance sensor is bolted to the top of the outer cylinder (983); The side wall of the contact needle (981) is fixedly connected to one end of the return spring (985), and the other end of the return spring (985) is fixedly connected to the guide cylinder (984). The contact needle (981) is slidably connected to the guide cylinder (984).

5. The EDTA decalcification device with multi-stage filtration and circulation functions according to claim 4, characterized in that: The guide tube (984) is connected to one end of the three-way solenoid valve (986), and the other end of the three-way solenoid valve (986) is connected to the first mating pipe (987); The other end of the three-way solenoid valve (986) is connected to the second mating pipe (988); Both the first connecting pipe (987) and the second connecting pipe (988) are equipped with one-way valves, and the upper ends of the first connecting pipe (987) and the second connecting pipe (988) are connected to the cavity (94).

6. The EDTA decalcification device with multi-stage filtration and circulation functions according to claim 5, characterized in that: A flow guide cavity (9811) is provided inside the contact needle (981), and the flow guide cavity (9811) is connected to the guide cylinder (984); The upper contact assembly (5) includes an upper receiving frame (51), an upper embedded frame (52) is fixedly connected to the side wall of the receiving frame (51), and the upper receiving frame (51) is fixedly connected to the sliding plate (4).

7. The EDTA decalcification device with multi-stage filtration and circulation functions according to claim 6, characterized in that: The upper receiving frame (51) consists of the lower receiving frame (93), the cavity (94) and the force-bearing component (98).

8. The EDTA decalcification device with multi-stage filtration and circulation functions according to claim 2, characterized in that: The first telescopic tube (6) and the second telescopic tube (7) are connected to the cavity (94) inside the upper receiving frame (51).

9. The EDTA decalcification device with multi-stage filtration and circulation functions according to claim 1, characterized in that: A valve (10) is provided at the bottom of the dissolving box (1), and the valve (10) is connected to the upper end of the filter box (11); The filter box (11) is equipped with multiple sets of filters (12), and the delivery pipe on the side wall of the filter box (11) is connected to the suction pump (13).

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