Tibetan medicine composition, preparation method and application of Tibetan medicine composition in antibacterial products
By optimizing the formulation and extraction process of traditional Tibetan medicine compositions, a highly efficient plant antibacterial agent was prepared, solving the problems of side effects and microbial resistance associated with chemical antibacterial agents, and achieving a broad-spectrum antibacterial effect against a variety of microorganisms.
Patent Information
- Application Number
- CN202511255200.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-04
- Publication Date
- 2026-01-23
AI Technical Summary
Existing chemical antibacterial agents have significant side effects, cause environmental pollution, are difficult to degrade, and easily lead to microbial resistance. Plant-based antibacterial agents have uneven effects and a limited range of antibacterial species.
An antibacterial agent was prepared by using a traditional Tibetan medicine composition of Sophora japonica, Phellodendron chinense, Myrica rubra, and Artemisia capillaris in a specific ratio, and then purifying it through ethanol extraction and water precipitation. The extraction process was optimized to increase the alkaloid content.
The obtained Tibetan medicine composition exhibits significant antibacterial effects against a variety of microorganisms, especially against Staphylococcus aureus and Streptococcus mutans, with an alkaloid content of 2.06-2.78 mg/g, making it suitable for various antibacterial products.
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Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of bacteriostatic agents and its application, and particularly relates to a Tibetan medicine composition, a preparation method and application thereof in bacteriostatic products. BACKGROUND
[0002] All the time, bacteriostatic agents play an indispensable important role in people's daily life. Chemical bacteriostatic agents have long occupied a dominant position. However, chemical bacteriostatic agents have greater side effects, and long-term use or improper use may harm human health, such as irritating the skin and mucous membranes, and even entering the human body through the respiratory tract, skin contact and other channels, affecting the normal physiological function of the human body. In addition, chemical bacteriostatic agents are difficult to degrade in the environment, easy to cause environmental pollution, and also make microorganisms resistant, leading to a gradual decline in bacteriostatic effect.
[0003] With the increasing concern of people on health and environmental protection, and the deepening understanding of the side effects of chemical bacteriostatic agents, it has become a research hotspot to find safe, environmentally friendly and effective bacteriostatic agents. Plant bacteriostatic agents have emerged as the times require. They are mainly extracted or synthesized from the roots, stems, leaves, flowers and fruits of plants, and have a wide range of sources. In addition, plant bacteriostatic agents contain alkaloids, flavonoids, phenols, terpenes and other components. These components mostly have antibacterial, anti-inflammatory, antiviral and other biological activities, and have many advantages such as high safety, good environmental protection and diverse bacteriostatic mechanisms.
[0004] However, bacteriostatic agents from different plant sources have large differences in bacteriostatic effect, and the bacteriostatic effect of common plant-derived bacteriostatic active ingredients is poor, and the inhibition of microorganisms is extremely limited. SUMMARY
[0005] In order to solve the above technical problems, the application provides a Tibetan medicine composition, a preparation method and application thereof in bacteriostatic products.
[0006] The Tibetan medicine composition provided by the application is composed of psammophyte, Chinese cork tree, water willow and artemisia.
[0007] Preferably, in the raw materials, the weight ratio of psammophyte: Chinese cork tree: water willow: artemisia is 1.5-3: 1.5-4: 1: 0.5-3.
[0008] The Tibetan medicine composition provided by the application is composed of traditional Tibetan medicine. In the composition: Sorbus, the dry seeds of the legume Sorbus, the alkaloids, flavonoids, esters, steroids and other bioactive ingredients in the rich Sorbus, currently, the pharmacological activity of Sorbus is mainly based on alkaloids, with good antibacterial, antiparasitic, antiviral / anti-tumor and other pharmacological activities.
[0009] Water willow, the tender branches of the plant Rhus chinensis, mainly growing in Qinghai, Gansu, Shaanxi, Sichuan, Tibet, Shanxi, Yunnan, etc. "Tibet commonly used Chinese herbal medicine" records that water willow has the effect of dispelling wind, relieving the surface, promoting rash, relieving cough, clearing heat and detoxifying.
[0010] Cortex phellodendri, the dried bark of the Rutaceae plant Phellodendron amurense, also known as "Sichuan Huangbai", "Clinical Commonly Used Chinese Herbal Medicine Handbook" records that Huangbai is bitter and cold, and has the effects of clearing heat and drying dampness, and strong fire and detoxification, and belongs to kidney and bladder channels, and is good at clearing lower damp heat.
[0011] Artemisia argyi, a one-year or two-year herbaceous plant of the family Compositae, contains volatile oil, lactones and azulenes, and has the effects of clearing heat and stopping bleeding.
[0012] The Tibetan medicine composition obtained by matching the above Tibetan medicine composition shows significant bacteriostatic effect, and the experimental results show that the bacteriostatic circle diameter of the Tibetan medicine composition to Staphylococcus aureus strain is as high as 24.83 mm.
[0013] The application also provides a preparation method of the above-mentioned Tibetan medicine composition, comprising the following steps: S1 raw material preparation: the Sorbus, Huangbai, water willow and Artemisia argyi are weighed according to the weight ratio, mixed uniformly, crushed, sieved, and the powder raw material is obtained; The weight ratio of Sorbus, Huangbai, water willow and Artemisia argyi is 1-3:1-4:1-2:0.5-3. S2 extraction: ethanol is added to the powder raw material obtained in S1, and reflux extraction is carried out for 2-4 times, each time for 1-2 h, and the filtrate is obtained by filtering the extraction liquid obtained each time, and the filtrate is combined and concentrated to obtain the ethanol extraction concentrate of the raw material; S3 purification: water is added to the ethanol extraction concentrate of S2, and the precipitate is removed by filtration, and the filtrate is dried to obtain the Tibetan medicine composition.
[0014] In the preparation method of the above-mentioned Tibetan medicine composition provided by the application, preferably, the mass-volume ratio of the powder raw material to ethanol in S2 is 1 g:8-20 mL.
[0015] Preferably, the volume fraction of ethanol in S2 is 60%-90%.
[0016] Preferably, the concentration in S2 is reduced by vacuum concentration, the temperature of vacuum concentration is 50-70 DEG C, and the concentration of the concentrated solution is 1-1.5 g / mL, so as to ensure that the concentrated solution is still in a solution state.
[0017] Preferably, the precipitation in S3 is performed by adding 10-30 times the volume of water to the concentrated solution and standing for 10-30 h, and the obtained filtrate is dried at 60-80 DEG C under reduced pressure.
[0018] The application also provides the use of the Tibetan medicine composition in the preparation of an antibacterial product.
[0019] Preferably, the antibacterial product is a product with antibacterial properties containing or loaded with the Tibetan medicine composition, and the product can include, but is not limited to, the following: antibacterial hand sanitizer, skin antibacterial cream or antibacterial cream, oral antibacterial product such as mouthwash or antibacterial lozenge, antibacterial laundry detergent or laundry detergent, air antibacterial spray, food preservative, food preservative, antibacterial textile.
[0020] The bacteria inhibited by the antibacterial product include, but are not limited to, the following: Escherichia coli, Candida albicans, Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes, Streptococcus mutans, Salmonella enteritidis, Pseudomonas aeruginosa, Porphyromonas gingivalis, Staphylococcus epidermidis.
[0021] Further, the application also provides a plant antibacterial agent, which comprises the above-mentioned Tibetan medicine composition.
[0022] Preferably, the plant antibacterial agent is prepared from the following raw materials by weight: 6.75 parts of desert locust, 12 parts of Chinese cork tree, 3 parts of water berberis, and 7.5 parts of large-seeded wormwood. The preparation method of the plant antibacterial agent is as follows: The above-mentioned raw materials such as desert locust, Chinese cork tree, water berberis, and large-seeded wormwood are weighed, mixed, and crushed, and then passed through a 20-mesh sieve. Then, 80% ethanol by volume is added, and the mixture is extracted twice. In the first extraction, the mass-volume ratio of the powder raw material to ethanol is 1 g:8 mL, and the extraction time is 1.5 h. In the second extraction, the mass-volume ratio of the powder raw material to ethanol is 1 g:6 mL, and the extraction time is 1 h. After the extraction liquid is filtered, the filtrate is combined, concentrated at 60 DEG C under reduced pressure to a relative density of 1.05 g / mL, and then 10 times the volume of water is added to the concentrated solution, and the mixture is precipitated for 16 h. The precipitate is removed by filtration, and the obtained filtrate is dried at 60 DEG C under reduced pressure to obtain the plant antibacterial agent.
[0023] The application has the following advantages: The traditional Tibetan medicine sand-husked sophora fruit, phellodendron, water berberis, and large-seeded wormwood are compounded, and active ingredients are extracted, so that a plant source bacteriostatic agent with good bacteriostatic effect is provided. In addition, the provided plant bacteriostatic agent shows good inhibition effect on ten kinds of microorganisms, including escherichia coli, candida albicans, staphylococcus aureus, bacillus subtilis, propionibacterium acnes, streptococcus mutans, salmonella enteritidis, pseudomonas aeruginosa, porphyromonas gingivalis, and staphylococcus epidermidis, and has the best inhibition effect on staphylococcus aureus and streptococcus mutans, with the inhibition ring diameters of 24.83 mm and 17.26 mm respectively. The plant bacteriostatic agent shows universal bacteriostatic property and can be widely applied to bacteriostatic products in various fields. BRIEF DESCRIPTION OF DRAWINGS
[0024] Figure 1 Inhibition effect of the Tibetan medicine composition of each example on staphylococcus aureus; Figure 2 Inhibition effect of the Tibetan medicine composition of each example on escherichia coli; Figure 3 Inhibition effect of the Tibetan medicine composition of each example on candida albicans; Figure 4 Inhibition effect of the Tibetan medicine composition of each comparative example on staphylococcus aureus; Figure 5 Inhibition effect of the Tibetan medicine composition of each comparative example on escherichia coli; Figure 6 Inhibition effect of the Tibetan medicine composition of each comparative example on candida albicans; Figure 7 Inhibition effect of the Tibetan medicine composition of comparative example 6 on staphylococcus aureus (A), candida albicans (B), and escherichia coli (C). DETAILED DESCRIPTION
[0025] In order to enable those skilled in the art to better understand the present application, the present application will be further described in conjunction with specific embodiments.
[0026] Examples 1-9 A Tibetan medicine composition, the preparation method is as follows: S1 raw material preparation: 6.75 parts of sand-husked sophora fruit, 12 parts of phellodendron, 3 parts of water berberis, and 7.5 parts of large-seeded wormwood are weighed, uniformly mixed, crushed, and passed through a 20-mesh sieve to obtain a powder raw material; S2 extraction: add 80% ethanol by volume to the powder raw material obtained in S1, and extract twice, the first time, the mass-volume ratio of powder raw material to ethanol is 1 g:8 mL, and the extraction time is 1.5 h, the second time, the mass-volume ratio of powder raw material to ethanol is 1 g:6 mL, and the extraction time is 1 h, after filtering the extraction liquid, combine the filtrate, and concentrate under reduced pressure at 60°C to obtain the ethanol extract concentrate of the raw material; S3 purification: add water to the ethanol extract concentrate of S2 to precipitate for a certain period of time, filter out the precipitate, and the filtrate is the water-soluble extract of the ethanol extract of S2, then the obtained filtrate is dried under reduced pressure at 60°C to obtain the water-soluble extract, which is the Tibetan medicine composition.
[0027] The antibacterial effect of the Tibetan medicine composition prepared in this example was evaluated by agar punching method, and the diameter of the antibacterial circle of the prepared Tibetan medicine composition was measured, and the specific experimental operation was as follows: On the clean bench, sterilized tryptone soy peptone agar medium (TSA) was cooled to about 60°C, 20 mL of culture medium was poured into a culture dish, and it was horizontally placed until it completely solidified, then 1 Oxford cup (inner diameter 6 mm, height 10 mm) was placed on the surface of the agar medium, and the agar hole was punched with a sterile needle, the culture medium in the agar hole was taken out with a sterile needle, 0.1 mL of the test bacterial suspension was taken on the plate, and the bacterial liquid was evenly coated with a coater to prepare a bacterial-containing plate, then the prepared Tibetan medicine composition sample (80 μL per hole) was injected in turn, and then the culture dish was placed in a 37°C constant temperature incubator for culture for 24 h, then the diameter of the antibacterial circle was measured with a vernier caliper, and 0.9% sterile sodium chloride solution was used as a blank control group.
[0028] The differences between Examples 1-9 are shown in Table 1.
[0029] Table 1 Differences and specific parameters of Examples 1-9 Concentration of the concentrated solution in step S2 (g / mL) Volume of water and precipitation time in step S3 Example 1 1.05 10 times volume of water precipitated for 16 h Example 2 1.05 20 times volume of water precipitated for 20 h Example 3 1.05 30 times volume of water precipitated for 24 h Example 4 1.10 20 times volume of water precipitated for 16 h Example 5 1.10 30 times volume of water precipitated for 20 h Example 6 1.10 10 times volume of water precipitated for 24 h Example 7 1.15 30 times volume of water precipitated for 16 h Example 8 1.15 10 times volume of water precipitated for 20 h Example 9 1.15 20 times volume of water precipitated for 24 h
[0030] The content of active ingredients in the Tibetan medicine composition prepared in each example is shown in Table 2.
[0031] Table 2 Content of active ingredients in the Tibetan medicine composition prepared in each example Number Yield of extract (%) Alkaloid content (mg / g) Number Yield of extract (%) Alkaloid content (mg / g) Example 1 10.49 2.20 Example 6 10.89 2.06 Example 2 12.02 2.23 Example 7 11.68 2.52 Example 3 11.29 2.37 Example 8 9.32 2.06 Example 4 13.25 2.78 Example 9 10.44 2.27 Example 5 11.04 2.08
[0032] As can be seen from Table 2, the concentration of the concentrate obtained when the Tibetan medicine composition is purified and the operation conditions of water precipitation will affect the obtained Tibetan medicine composition and the content of alkaloid active ingredients in it, among which, the Tibetan medicine composition obtained by using the conditions of Example 4 has the highest quality, and the content of alkaloid active ingredients in the Tibetan medicine composition is also the highest.
[0033] The antibacterial properties of the Tibetan medicine compositions prepared in the above examples were determined, and the experimental results are shown in Table 3 below.
[0034] Table 3 Antibacterial effects of the Tibetan medicine compositions of the examples on various microorganisms Strain Escherichia coli Index Inhibition zone diameter (mm) Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Example 1 8.6 8.4 8.2 8.4±0.2 Example 2 8.4 8.3 8.1 8.27±0.15 Example 3 8.2 8.3 8.3 8.27±0.06 Example 4 8.5 8.4 8.2 8.37±0.15 Example 5 8.3 8 8.6 8.3±0.3 Example 6 8.3 8.4 8.4 8.37±0.06 Example 7 8.5 8.2 8.2 8.3±0.17 Example 8 8.1 8.2 8.3 8.2±0.1 Example 9 8.2 8.3 8.1 8.2±0.1 Strain Staphylococcus aureus Index Inhibition zone diameter (mm) Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Example 1 24.2 23.1 24 23.77±0.59 Example 2 23.2 22.9 23.5 23.2±0.3 Example 3 23.2 22.9 22.1 22.73±0.57 Example 4 24.9 24.8 24.8 24.83±0.06 Example 5 24.5 22.8 23.9 23.73±0.86 Example 6 23.9 23.2 23.9 23.67±0.4 Example 7 23.9 23.7 23.2 23.6±0.36 Example 8 23.9 23.6 23.5 23.67±0.21 Example 9 24.1 24 23.8 23.97±0.15 Strain Candida albicans Index Inhibition zone diameter (mm) Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Example 1 12.1 11.7 12 11.93±0.21 Example 2 11.8 12.3 11.6 11.9±0.36 Example 3 11 11.6 12.4 11.67±0.7 Example 4 13 12.1 12.6 12.57±0.45 Example 5 12.3 11.9 12.4 12.2±0.26 Example 6 11.2 12.1 11.2 11.5±0.52 Example 7 10.9 11.3 12.3 11.5±0.72 Example 8 12.3 12.1 12 12.13±0.15 Example 9 12 11.9 12.2 12.03±0.15
[0035] In addition, Figure 1-3 The antibacterial effects of the Tibetan medicine compositions of Examples 1-9 on Staphylococcus aureus, Escherichia coli, and Candida albicans are shown in the following tables, respectively.
[0036] Figure 1-3 In the tables, A, B, and C represent three groups of parallel tests, respectively.
[0037] The experimental data in the above tables and Figure 1-3 The results show that the Tibetan medicine compositions obtained in the examples exhibit universal antibacterial properties, and have the best inhibitory effect on Staphylococcus aureus, followed by Candida albicans, and then Escherichia coli.
[0038] In addition, the antibacterial effects of the Tibetan medicine composition prepared in Example 4 on different bacteria are shown in Table 4 below.
[0039] Table 4 Antibacterial effects of the Tibetan medicine composition of Example 4 (diameter of inhibition zone, mm) Strain Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Staphylococcus aureus 24.9 24.8 24.8 24.83±0.06 Staphylococcus epidermidis 15.17 15.65 15.02 15.28±0.33 Bacillus subtilis 11.42 11.18 11.59 11.4±0.21 Propionibacterium acnes 8.00 8.00 8.00 8.00±0 Streptococcus mutans 17.25 17.32 17.21 17.26±0.06 Salmonella enteritidis 9.19 9.15 9.24 9.19±0.05 Pseudomonas aeruginosa 8.72 8.99 8.85 8.85±0.14 Escherichia coli 8.5 8.4 8.2 8.37±0.15 Porphyromonas gingivalis 13.76 13.09 12.96 13.27±0.43 Candida albicans 13 12.1 12.6 12.57±0.45
[0040] The results in Table 4 show that the Tibetan medicine composition prepared in the application has good inhibitory effect on a variety of microorganisms, and can be used as an antibacterial agent for a variety of microorganisms.
[0041] Comparative Examples 1-9 A Tibetan medicine composition was prepared by the following method: S1 Raw material preparation: The raw materials of Amorphaea fruticosa, Phellodendron amurense, Myricaria pinnata, and Artemisia japonica were weighed according to the proportions, mixed, ground, and sieved through a 20-mesh sieve to obtain a powder raw material; S2 The same as Examples 1-9; S3 Unlike the examples, the ethanol solution of each Tibetan medicine composition was not purified by water precipitation, but was directly used in the subsequent antibacterial experiments.
[0042] The weight ratios of the raw materials in Comparative Examples 1-9 are shown in Table 5 below.
[0043] Table 5 Raw material proportions of the comparative examples Sophora alopecuroides Phellodendron amurense Myricaria laxiflora Artemisia sphaerocephala Comparative example 1 4.5 3 3 6 Comparative example 2 4.5 7.5 6.5 7.5 Comparative example 3 4.5 12 10 9 Comparative example 4 6.75 3 6 9 Comparative example 5 6.75 7.5 10 6 Comparative example 6 6.75 12 3 7.5 Comparative example 7 9 3 10 7.5 Comparative example 8 9 7.5 3 9 Comparative example 9 9 12 6.5 6
[0044] The content of the active ingredients in the Tibetan medicine compositions prepared in the comparative examples is shown in Table 6 below.
[0045] Table 6: Content of active ingredients in the Tibetan medicine compositions prepared in the various comparative examples Number Yield of extract (%) Alkaloid content (mg / g) Number Yield of extract (%) Alkaloid content (mg / g) Comparative example 1 18.72 1.11 Comparative example 6 18.54 1.70 Comparative example 2 19.26 1.25 Comparative example 7 21.16 1.00 Comparative example 3 20.21 1.58 Comparative example 8 17.66 1.56 Comparative example 4 18.02 1.04 Comparative example 9 19.45 1.70 Comparative example 5 19.88 1.22
[0046] As can be seen from the data in Table 6, the extract content and the content of active ingredients in the extract of the Tibetan medicine compositions obtained under different proportions all showed great differences. Among them, the antibacterial effect of the Tibetan medicine composition obtained under the proportion of 6.75 parts of haloxylon ammodendron, 12 parts of phellodendron, 3 parts of water berberis, and 7.5 parts of artemisia was the best.
[0047] The antibacterial effect of the Tibetan medicine compositions of the various comparative examples on various microbial strains is shown in Table 7 below.
[0048] Table 7: Antibacterial effect of the Tibetan medicine compositions of the various comparative examples on various microbial strains Strain Staphylococcus aureus Index Inhibition zone diameter (mm) Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Comparative example 1 17.56 17.57 17.4 17.51±0.1 Comparative example 2 16.38 16.58 16.58 16.51±0.12 Comparative example 3 15.51 14.79 15.45 15.25±0.4 Comparative example 4 13.73 13.61 13.86 13.73±0.13 Comparative example 5 11.32 11.98 12.34 11.88±0.52 Comparative example 6 21.51 21.43 22.41 21.78±0.54 Comparative example 7 8.76 8.94 8.17 8.62±0.4 Comparative example 8 19.43 19.33 19.44 19.4±0.06 Comparative example 9 18.08 18.45 18.36 18.3±0.19 Strain Escherichia coli Index Inhibition zone diameter (mm) Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Comparative example 1 8.21 8.31 8.35 8.29±0.07 Comparative example 2 8.35 8.35 8.25 8.32±0.06 Comparative example 3 8 8 8 8±0 Comparative example 4 8.35 8.31 8.21 8.29±0.07 Comparative example 5 8 8 8 8±0 Comparative example 6 8.67 8.7 8.92 8.76±0.14 Comparative example 7 8 8 8 8±0 Comparative example 8 8.86 8.95 8.99 8.93±0.07 Comparative example 9 8.76 8.65 8.5 8.64±0.13 Strain Candida albicans Index Inhibition zone diameter (mm) Replicate hole 1 Replicate hole 2 Replicate hole 3 Average value Comparative example 1 8.53 8.72 8.62 8.62±0.1 Comparative example 2 8.32 8.72 8.52 8.52±0.2 Comparative example 3 9.85 9.38 9.62 9.62±0.24 Comparative example 4 9.95 9.68 9.62 9.75±0.18 Comparative example 5 9.89 9.35 9.35 9.53±0.31 Comparative example 6 15.98 16.2 15.91 16.03±0.15 Comparative example 7 9.21 8.72 8.83 8.92±0.26 Comparative example 8 10.8 10.67 10.81 10.76±0.08 Comparative example 9 9.26 9.53 9.26 9.35±0.16
[0049] Figure 4-6 The inhibitory effect of the Tibetan medicine compositions prepared in Comparative Examples 1-9 without water deposition purification on Staphylococcus aureus, Escherichia coli, and Candida albicans is shown, respectively, wherein A, B, and C are three groups of corresponding parallel experiments.
[0050] The results of Table 7 and Figure 4-6 show that under the proportion of Comparative Example 6, i.e., the weight ratio of haloxylon ammodendron: phellodendron: water berberis: artemisia = 6.75: 12: 3: 7.5, even without water deposition purification treatment, the antibacterial effect of the ethanol extract of the obtained Tibetan medicine composition on each microbial strain is the best, followed by the proportions of Comparative Example 8 and Comparative Example 9.
[0051] In addition, in order to more clearly compare the inhibitory effect of the Tibetan medicine composition obtained in Comparative Example 6 on Staphylococcus aureus, Candida albicans, and Escherichia coli strains, a separate comparison was made, as shown in Figure 7 , wherein A, B, and C are three groups of parallel experiments for verifying the inhibitory effect of the Tibetan medicine composition of Comparative Example 6 on Staphylococcus aureus, Candida albicans, and Escherichia coli, respectively. The comparison results clearly show that the Tibetan medicine composition has a significant inhibitory effect on Staphylococcus aureus and Candida albicans compared to Escherichia coli and other strains.
[0052] Example 10 This example focuses on optimizing the extraction process of the Tibetan medicine composition. Table 8 is the orthogonal test factor level table, Table 9 is the orthogonal test table, and Table 10 shows the orthogonal test results of the extraction process optimization.
[0053] Table 8: Orthogonal factor level table Factor Material-liquid ratio (A) Time (B) Ethanol concentration (C) 1 1：（8、4） 1+1 60% 2 1：（8、6） 1.5+1 70% 3 1：（8、8） 2+1 80%
[0054] The 1: (8, 4) in the material-liquid ratio of Table 8 refers to the material-liquid ratio of 1 g:8 mL and 1 g:4 mL in the two times of extraction, and the same applies to the other; the 1+1 in the time refers to the time length of 1 h and 1 h in the two times of extraction, and the same applies to the other.
[0055] Table 9 Orthogonal experiment table Experiment number A B C D (error) 1 1 1 1 1 2 1 2 2 2 3 1 3 3 3 4 2 1 2 3 5 2 2 3 1 6 2 3 1 2 7 3 1 3 2 8 3 2 1 3 9 3 3 2 1
[0056] Table 10 Orthogonal experiment results
[0057] It can be seen from Tables 8-10 that the alkaloid content of A1B3C3 is the highest, and therefore, the optimal process condition is that the material-liquid ratio is 1:8 in the first extraction, the material-liquid ratio is 1:6 in the second extraction, the extraction time is 1.5 h in the first extraction and 1 h in the second extraction, and the ethanol concentration is 80%.
Claims
1. A Tibetan medicine composition, characterized in that, The raw materials are composed of desert locust tree, phellodendron, water berberis, and artemisia.
2. The Tibetan medicine composition according to claim 1, wherein, The weight ratio of desert locust tree, phellodendron, water berberis, and artemisia in the raw materials is 1-3:1-4:1-2:0.5-5.
3. A process for the preparation of the Tibetan medicine composition as claimed in any one of claims 1-2, characterized in that, The steps include the following: S1 raw material preparation: desert locust tree, phellodendron, water berberis, and artemisia are weighed according to the weight ratio, mixed uniformly, crushed, sieved, and powder raw materials are obtained; The weight ratio of desert locust tree, phellodendron, water berberis, and artemisia is 1-3:1-4:1-2:0.5-3; S2 extraction: ethanol is added to the powder raw materials obtained in S1, and reflux extraction is performed for 2-4 times, each time for 1-2 hours; the filtrate obtained by filtering the extraction liquid each time is combined, concentrated, and the ethanol extraction concentrate of the raw material is obtained; S3 purification: water is added to the ethanol extraction concentrate of S2, the precipitate is removed by filtration, and the obtained filtrate is dried to obtain the Tibetan medicine composition.
4. The production method according to claim 3, wherein The mass-volume ratio of the powder raw materials to ethanol in S2 is 1 g:8-20 mL.
5. The production method according to claim 3, wherein The volume fraction of ethanol in S2 is 60%-90%.
6. The production method according to claim 3, wherein In S2, the temperature of the reduced pressure concentration is 50-70℃, and the concentration liquid is concentrated to a concentration of 1-1.5 g / mL.
7. The production method according to claim 3, wherein In S3, the precipitate is precipitated with 10-30 times the volume of water for 10-30 hours, and the obtained filtrate is dried at a low temperature under reduced pressure at 60-80℃.
8. Use of the Tibetan medicine composition according to any one of claims 1-2 for the preparation of an antibacterial product, characterized in that, The antibacterial products include but are not limited to the following: antibacterial hand sanitizer, skin antibacterial cream or antibacterial cream, oral antibacterial product, antibacterial laundry detergent or laundry detergent, air antibacterial spray, food preservative, food preservative, antibacterial textile.
9. Use according to claim 8, wherein the compound is ###0002### The bacteria inhibited by the antibacterial products include but are not limited to the following: Escherichia coli, Candida albicans, Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes, Streptococcus mutans, Salmonella enteritidis, Pseudomonas aeruginosa, Porphyromonas gingivalis, and Staphylococcus epidermidis.
10. A plant bacteriostatic agent, characterized by, The plant antibacterial agent is prepared by using the following raw materials: The plant antibacterial agent is prepared by using the following raw materials: The above raw materials are weighed, mixed uniformly, crushed, and sieved through a 20-mesh sieve, then 80% ethanol is added, and extracted twice; the mass-volume ratio of the powder raw materials to ethanol is 1 g:8 mL for the first extraction, and the extraction time is 1.5 hours; the mass-volume ratio of the powder raw materials to ethanol is 1 g:6 mL for the second extraction, and the extraction time is 1 hour; the filtrate is combined after filtering the extraction liquid, and concentrated under reduced pressure at 60℃ until the concentration of the concentrated liquid is 1.05 g / mL; finally, 10 times the volume of water is added to the concentrated liquid, precipitated for 16 hours, and the precipitate is removed by filtration; the obtained filtrate is dried at a low temperature under reduced pressure at 60℃ to obtain the plant antibacterial agent.