Traditional Chinese medicine compound formula for disinfecting silkworm rearing bed bacterial disease pathogens as well as preparation and application of traditional Chinese medicine compound formula

Abstract

The invention discloses a traditional Chinese medicine compound formula for disinfecting silkworm rearing bacterial disease pathogens and preparation and application of the traditional Chinese medicine compound formula. Wormwood, dandelion, honeysuckle, golden cypress and radix sophorae flavescentis are mixed to obtain the traditional Chinese medicine compound formula for disinfecting the silkworm rearing bacterial disease pathogens; in the 1-3-year-old silkworm period, the silkworm rearing bed is disinfected once every two days through the spray decoction; in the adult silkworm period of 4-5 years old, the silkworm rearing period of 4 years old and the full feeding period of 5 years old, the dry powder disinfectant is used for disinfecting the silkworm rearing bed once. The invention discloses a traditional Chinese medicine compound formula for disinfecting silkworm rearing bacterial disease pathogens and application of the traditional Chinese medicine compound formula in silkworm breeding, particularly silkworm rearing disinfection for the first time. Silkworm bacterial diseases are common silkworm diseases, chemical drugs are used for disinfection in the prior art, application of silk in the medical beauty industry is limited, different technical thoughts are adopted, wormwood, dandelion, honeysuckle, golden cypress and radix sophorae flavescentis are cooperatively matched, different disinfectant forms in the silkworm period and the adult silkworm period are combined, and the disinfection effect better than that in the prior art is unexpectedly achieved; the application of the silk in the medical beauty industry is not influenced.

Description

TECHNICAL FIELD

[0001] The application belongs to silkworm breeding technology, and relates to antibacterial treatment, in particular to a silkworm seat bacterial disease pathogen disinfection traditional Chinese medicine compound formula and preparation and application thereof. BACKGROUND

[0002] Silkworm bacterial disease is a common silkworm disease, mainly including four categories of bacillus thuringiensis, bacillus mesonale, spirit and fecal streptococcus, due to the wide source of pathogens, various types, due to the factors inducing bacterial diseases, including high temperature, high humidity, fermented mulberry leaves, etc. In the past, chloramphenicol was used for prevention and treatment, due to the prohibition of use, at present, mainly using chlorine-containing disinfectants (such as bleaching powder) or formaldehyde solution for disinfection before silkworm breeding, using low-concentration disinfectants (such as 0.3% peracetic acid) for silkworm seat disinfection during silkworm breeding, or adding food such as florfenicol antibiotic, using lime or chlorine-containing disinfectants (such as bleaching powder) for silkworm seat and ground disinfection after silkworm breeding. For example, CN106172267A discloses a silkworm breeding method using new bleaching powder and fresh lime powder to disinfect silkworm body and silkworm seat; CN102939976A uses carbendazim as silkworm seat disinfection isolation agent; CN107361030A discloses a high-efficiency silkworm breeding disinfection method using compound bleaching powder, and the mixed and uniform disinfection lime powder is uniformly spread on the silkworm seat; CN101027997A discloses a silkworm breeding disinfectant, which is prepared by heating, dissolving, mixing and cooling a mixture of dodecyl dimethyl benzyl ammonium chloride, benzalkonium bromide, polyethoxyl carboxyl guanidine and ethanol.

[0003] With the application of silk products in the medical field, the production of medical-grade silk protein raw materials has higher requirements for the environment of silkworm breeding process, not only requiring no infection of bacterial diseases, but also requiring no use of chemical drugs for preventing and treating bacterial diseases throughout the process, based on this, the application develops a traditional Chinese medicine compound formula. SUMMARY

[0004] The application takes "broad-spectrum antibacterial, silkworm safety, easy to obtain" as the core design principle, discloses common and low-cost herbal medicines, enhances the disinfection effect on silkworm seats through compound synergy, and has no acute toxicity and no antifeeding to silkworm larvae (1-5 years old) through testing, and the cost is only 1 / 3-1 / 2 of the existing chemical disinfectants (such as sodium hypochlorite and peracetic acid), especially, the traditional Chinese medicine compound formula in the application does not limit the application of silk products in the medical field after being used for silkworm seat bacterial disease pathogen disinfection.

[0005] The application adopts the following technical scheme.

[0006] A silkworm seat bacterial disease pathogen disinfection traditional Chinese medicine compound formula, comprising wormwood, dandelion, honeysuckle, Chinese cork tree and sophora.

[0007] This invention discloses a method for preparing the above-mentioned traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm bursal bacterial disease, including the following steps: mixing Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens to obtain the traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm bursal bacterial disease.

[0008] Furthermore, the preparation method of the above-mentioned traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm larvae bacterial disease includes the following steps: mixing Artemisia argyi, dandelion, honeysuckle, Phellodendron chinense, and Sophora flavescens and boiling them in water, then removing the dregs to obtain the traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm larvae bacterial disease, which is a spray decoction; this formula is used for 1st to 3rd instar silkworms.

[0009] Furthermore, the preparation method of the above-mentioned traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm larvae bacterial disease includes the following steps: mixing Artemisia argyi, dandelion, honeysuckle, Phellodendron chinense, and Sophora flavescens in powder form to obtain the traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm larvae bacterial disease, which is a dry powder disinfectant; this formula is used for 4-5 instar silkworms.

[0010] In this invention, the mass ratio of Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens is (25-35):(20-30):(15-25):(10-20):(5-15); preferably, the mass ratio of Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens is (28-32):(22-27):(18-23):(13-18):(8-12); for example, the mass ratio of Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens is 30:25:20:15:10.

[0011] This invention discloses a method for disinfecting pathogens causing bacterial diseases in silkworm beds, comprising the following steps: using the above-mentioned traditional Chinese medicine compound formula for disinfecting pathogens causing bacterial diseases in silkworm beds.

[0012] Furthermore, in the above-mentioned methods for disinfecting silkworm bed bacterial diseases, for 1st-3rd instar silkworms, a spray decoction is used at 15-20 mL / m². 2 For silkworms in their 4th-5th instar stage, use a dry powder disinfectant. Apply it directly and evenly to the surface of the silkworm bed, using 5-8g per square meter.

[0013] Specifically, during the 1st to 3rd instar silkworm stage, the silkworm bed should be disinfected with a spray decoction every 2 days; during the 4th to 5th instar silkworm stage, the silkworm bed should be disinfected with a dry powder disinfectant once each during the 4th instar silkworm emergence period and the 5th instar peak feeding period (3-4 days after the 5th instar).

[0014] The application discloses a compound formula of a disinfectant for silkworm seat bacterial disease pathogens and application of the disinfectant in silkworm breeding, specifically in silkworm seat disinfection. Silkworm bacterial disease is a common silkworm disease, and the existing technology uses chemical drugs for disinfection, which limits the application of silk in the medical and beauty industries. The application adopts a different technical idea, and uses wormwood, dandelion, honeysuckle, Chinese cork tree and sophora flavescens in cooperation, and combines different disinfectant formulations in the small silkworm stage and the large silkworm stage, so that better disinfection effect is unexpectedly achieved than that of the prior art, and the application of silk in the medical and beauty industries is not affected. DETAILED DESCRIPTION

[0015] The application takes “broad-spectrum bacteriostasis, silkworm safety and easy source” as the core design principle, discloses common and low-cost herbal medicines, and enhances the disinfection effect on silkworm seats through compound cooperation. The herbal medicines are tested to be free of acute toxicity and food rejection to silkworm larvae (1-5 instars), and the cost is only 1 / 3-1 / 2 of that of the existing chemical disinfectants (such as sodium hypochlorite and peroxoacetic acid). In particular, the application of the herbal compound formula to silkworm seat bacterial disease pathogen disinfection does not limit the application of silk products in the medical field.

[0016] The technical progress of the application is illustrated by specific experiments. The herbal medicines used are all commercially available products, which meet the conventional requirements of traditional Chinese medicines. The specific preparation operation and silkworm breeding method are conventional technologies.

[0017] Wormwood (dry product) has very low cost, and the annual yield of dry product per mu is more than 500 kg, and the unit price is about 2 yuan / kg; dandelion (dry product of whole grass) is large-scale planted, and the unit price of dry product is about 3 yuan / kg; honeysuckle (dry product of flower bud) is large-scale planted, and the unit price of dry product is about 8 yuan / kg (much lower than other antibacterial traditional Chinese medicines); Chinese cork tree (dry product of tree bark, silk) can be recycled to collect tree bark (without damaging trees), and the unit price of dry product is about 10 yuan / kg; sophora flavescens (dry product of root, slice) can be planted on marginal land, and the unit price of dry product is about 6 yuan / kg.

[0018] Embodiment Two practical formulations suitable for different silkworm seat scenes The mass ratio of wormwood, dandelion, honeysuckle, Chinese cork tree and sophora flavescens is 30:25:20:15:10.

[0019] 1000 g of the above-mentioned traditional Chinese medicines are weighed according to the proportion, crushed into coarse powder (particle diameter 1-2 mm), 10 L of water is added, soaked for 30 minutes, boiled with large fire, then transferred to small fire and boiled for 40 minutes, avoid paste at the bottom during the process, turn off the fire and stand for 15 minutes, filter twice with gauze (80 meshes) to remove the dregs, and about 8 L of decoction is obtained, then dilute with water according to the mass ratio of 1:1 after natural cooling to room temperature, and then pour into a sprayer for use. The prepared silkworm seat spray disinfectant is suitable for 1-3 instar silkworms, and the dosage is 15 mL / m 2.

[0020] Artemisia, dandelion, honeysuckle, Chinese cork tree, Sophora flavescens were crushed into fine powder (100 mesh); after mixing uniformly according to the proportion, it was put into an iron pan and fried for 10 minutes (the temperature was controlled at 65 DEG C), and then it was cooled naturally and stored in a sealed bag. The prepared silkworm seat dry powder disinfectant is suitable for 4-5 age large silkworms; when used, it is directly and uniformly applied on the surface of the silkworm seat, and 6g per square meter of silkworm seat is applied.

[0021] Method for use: Spray decoction: used after cleaning the silkworm seat and before feeding, used for 1 age silkworms, once every 2 days to the end of 3 age; Dry powder disinfectant: used for 4 age silkworms, 5 age 3 days each once, fed after 30 minutes after application to avoid silkworms eating too much dry powder.

[0022] After the compound formula spray or application of the application for 24 hours, the silkworms have no death, no deformity and normal feeding, which indicates that the formula is safe.

[0023] Comparison of existing main disinfection methods (florfenicol, bleaching powder, lime powder).

[0024] 20% florfenicol soluble powder is used, 0.5g of the powder is added into 10L of clean water and stirred until completely dissolved, the liquid is filled into a small sprayer, and the silkworm seat is uniformly sprayed, which is suitable for 3-5 age, 15mL / m 2 , used for 3 age silkworms, once every 3 days to the end of 5 age.

[0025] 25% effective chlorine bleaching powder is used, and a water solution containing 1% is configured, the liquid is filled into a small sprayer, and the silkworm seat is uniformly sprayed and disinfected, 15mL / m 2 , used for 3 age silkworms, once every 2 days to the end of 5 age.

[0026] Before feeding mulberry, fresh lime powder is evenly scattered on the silkworm seat, and the silkworm net is added, and then the silkworms are normally fed after climbing onto the net; 8g per square meter of silkworm seat is applied, and the silkworms are used once at 3 age, 4 age and 5 age respectively.

[0027] The existing traditional Chinese medicine disinfection is mainly single "bacteriostatic" function, the formula combines "volatile component space disinfection + contact bacteriostasis + hygroscopic component to improve the environment", and solves the problems of bacteria survival, transmission and breeding; moreover, the application avoids high-cost varieties such as traditional antibacterial traditional Chinese medicine, and selects "field easy-to-get type" herbs such as Artemisia and dandelion, so that the cost of per mu of silkworm and mulberry can be controlled within 20 yuan; in particular, the compound of the application avoids stimulating the digestive tract of silkworms and ensures the feeding enthusiasm of silkworms.

[0028] Example: The silkworm variety "Jingsong × Haoyue" was used. Each area was reared on one sheet (30,000 silkworms, divided into 50 sheets). The mulberry variety was Yu 711. The experiment was conducted during the autumn silkworm season of 2025 at Jiayuan Sericulture Farm, Gujin Village, Tangyang Town, Dongtai City, Jiangsu Province. The criteria for identifying bacterial diseases were based on the symptoms of diseased silkworms, including shortened body length, loss of appetite, loose excrement, and watermarks on the tail. The incidence rate was recorded per sheet (bacterial diseased silkworms / total number of silkworms). Parallel experiments were conducted in each group.

[0029] Table 1. Results of the incidence rate survey

[0030] Note: Existing medications are not suitable for treating very small silkworms.

[0031] Comparative Example 1 Referring to the example, the mass ratio of Artemisia argyi, dandelion, honeysuckle, Phellodendron amurense, and Sophora flavescens was 25:30:10:20:15. After the first spray disinfection, it was found that this formula affected the feeding of silkworms, so it was abandoned and the traditional method was used instead.

[0032] Comparative Example 2 Referring to the example, except that dandelion was replaced with Isatis tinctoria, the rest remained the same; the incidence of bacterial disease was 2.7-3.3%.

[0033] Comparative Example 3 Referring to the example, Sophora flavescens was omitted, and the mass ratio of Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens was 35:30:20:15, with the rest being the same; the incidence rate of bacterial disease was 2.5-3.1%.

[0034] Comparative Example 4 Referring to the example, the first three instar silkworms were also initially treated with a dry powder disinfectant for the silkworm bed, with 3g applied per square meter of the silkworm bed. However, it was found that the powder affected the silkworms' feeding, so the method was abandoned and replaced with the traditional method.

[0035] Comparative Example 5 Referring to the example, 4-5 instar silkworms also used spray disinfectant decoction, and the rest was the same; the incidence of bacterial disease was 2.1-2.7%.

[0036] This invention's compound formula can disrupt bacterial cell membranes while simultaneously absorbing moisture and preventing dampness, reducing the environment conducive to bacterial growth on silkworm beds. It has a strong inhibitory effect on the main pathogens of silkworm bed bacterial diseases, is easily absorbed by the silkworm bed, avoids irritating the silkworm's digestive tract, exhibits high stability, and can also mildly repel silkworm bed pests such as mites, reducing the spread of bacteria carried by pests. This invention solves the problems of antibiotic residues or poor efficacy in existing drug treatments, and further addresses the issue that existing methods cannot meet the production environment requirements of medical aesthetics.

Claims

1. A traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm scabies, comprising Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens.

2. The traditional Chinese medicine compound formula for disinfecting silkworm disease pathogens according to claim 1, characterized in that, The mass ratio of Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens is (25-35): (20-30): (15-25): (10-20): (5-15).

3. The traditional Chinese medicine compound formula for disinfecting silkworm disease pathogens according to claim 1, characterized in that, The mass ratio of Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens is (28-32): (22-27): (18-23): (13-18): (8-12).

4. The preparation method of the traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm bursal bacterial disease as described in claim 1 includes the following steps: mixing Artemisia argyi, Taraxacum mongolicum, Lonicera japonica, Phellodendron chinense, and Sophora flavescens to obtain the traditional Chinese medicine compound formula for disinfecting the pathogen of silkworm bursal bacterial disease.

5. The preparation method of the traditional Chinese medicine compound formula for disinfecting silkworm disease pathogens according to claim 4, characterized in that, The preparation method includes the following steps: mixing mugwort, dandelion, honeysuckle, phellodendron bark, and sophora flavescens, boiling them in water, and then removing the dregs to obtain a traditional Chinese medicine compound formula for disinfecting the pathogens of silkworm cocoon bacterial diseases. This formula is a spray decoction and is used for 1st to 3rd instar silkworms. Alternatively, the preparation method of the traditional Chinese medicine compound formula for disinfecting the pathogens of silkworm cocoon bacterial diseases includes the following steps: mixing mugwort, dandelion, honeysuckle, phellodendron bark, and sophora flavescens in powder form to obtain a traditional Chinese medicine compound formula for disinfecting the pathogens of silkworm cocoon bacterial diseases. This formula is a dry powder disinfectant and is used for 4th to 5th instar silkworms.

6. A method for disinfecting pathogens causing bacterial diseases in silkworm beds, comprising the following steps: using the traditional Chinese medicine compound formula for disinfecting pathogens causing bacterial diseases in silkworm beds as described in claim 1.

7. The method for disinfecting silkworm rearing bacterial pathogens according to claim 6, characterized in that, In the disinfection methods for bacterial diseases in silkworms, spray decoction is used for the 1st to 3rd instar silkworms, and dry powder disinfectant is used for the 4th to 5th instar silkworms.

8. The method for disinfecting silkworm rearing bacterial pathogens according to claim 7, characterized in that, During the first to third instar silkworm stages, disinfect the silkworm rearing area with a spray decoction every two days; during the fourth to fifth instar silkworm stages, disinfect the silkworm rearing area with a dry powder disinfectant once at the start of the fourth instar and once during the peak feeding period of the fifth instar.

9. The application of the traditional Chinese medicine compound formula for disinfecting the pathogens of silkworm disease as described in claim 1 in silkworm breeding.

10. The application of the traditional Chinese medicine compound formula for disinfecting silkworm bed bacterial pathogens as described in claim 1 in the disinfection of silkworm beds.

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