Bovine bone marrow and grape seed vitamin capsule and preparation method thereof

Abstract

The invention relates to the field of food health care, in particular to bovine bone marrow and grape seed vitamin capsules and a preparation method thereof. The bovine bone marrow, grape seed and vitamin capsule comprises a hollow capsule and capsule content, and the capsule content comprises the following raw materials in percentage by weight: 30-50% of bovine bone marrow microcapsule powder, 10-20% of grape seed extract, 5-10% of vitamin C, 2-5% of vitamin E, 0.01-0.05% of vitamin D3 and the balance of auxiliary materials, totaling 100%. Wherein the bovine bone marrow microcapsule powder is prepared by the following steps: (1) cleaning and crushing fresh bovine bone marrow, and performing vacuum drying under the condition that the temperature is less than or equal to 60 DEG C; (2) extracting bovine bone marrow oil from the dried bovine bone marrow by using a supercritical CO2 extraction technology; and (3) carrying out microcapsule embedding treatment on the bone marrow oil by taking modified starch and maltodextrin as wall materials to obtain the bovine bone marrow microcapsule powder. The comprehensive effects of nutrition supplement and function assistance are realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food health care, in particular to a bovine bone marrow grape seed vitamin capsule and a preparation method thereof. BACKGROUND

[0002] With the improvement of people's health awareness, there is an increasing demand for health food with functions of antioxidant, immunity enhancement, and bone density improvement. Bovine bone marrow is rich in fat-soluble vitamins, phospholipids, cholesterol, and various trace elements, and has the traditional functions of nourishing yin and kidney and strengthening muscles and bones; grape seed extract is rich in proanthocyanidins (OPC) and has the functions of strong antioxidant, free radical scavenging, and cardiovascular protection; vitamins (such as vitamin C, E, D, etc.) play an important role in human metabolism, immune regulation, and antioxidant processes.

[0003] However, there is still a lack of capsule products on the market that scientifically combine bovine bone marrow, grape seed extract, and vitamins to synergistically exert antioxidant and bone health functions. In addition, bovine bone marrow oil has a high fat content and is prone to oxidation and deterioration, and traditional extraction and preservation methods are difficult to maintain its active ingredients, limiting its application in modern health products. SUMMARY

[0004] In order to maintain the activity of bovine bone marrow and provide a multifunctional product with antioxidant and bone health functions, the present application provides a bovine bone marrow grape seed vitamin capsule and a preparation method thereof.

[0005] In a first aspect, the present application provides a bovine bone marrow grape seed vitamin capsule, which adopts the following technical solution.

[0006] A bovine bone marrow grape seed vitamin capsule, comprising a hollow capsule and a capsule content, wherein the capsule content comprises the following raw materials in weight percentage:

[0007] 30-50% of bovine bone marrow microcapsule powder, 10-20% of grape seed extract, 5-10% of vitamin C, 2-5% of vitamin E, 0.01-0.05% of vitamin D3, and the balance of auxiliary materials to 100%;

[0008] The bovine bone marrow microcapsule powder is prepared by the following steps:

[0009] (1) Fresh bovine bone marrow is washed and broken, and then vacuum dried at a temperature of ≤60℃;

[0010] (2) The dried bovine bone marrow is extracted with supercritical CO2 extraction technology to obtain bovine bone marrow oil;

[0011] (3) The bone marrow oil is subjected to microcapsule embedding treatment using denatured starch and malt dextrin as wall materials to obtain bovine bone marrow microcapsule powder.

[0012] By adopting the technical scheme, the heat-sensitive nutritional ingredients (such as phospholipids and fatty acids) can be prevented from being destroyed by vacuum drying of the bovine bone marrow at ≤60°C, the supercritical CO2 extraction technology can efficiently extract the bovine bone marrow oil without solvent residue, and then the denatured starch and malt dextrin are used for microencapsulation, which can not only isolate oxygen and light to prevent the oxidation and deterioration of the bovine bone marrow oil, but also can refine the particles to improve the intestinal absorption efficiency and mask the fishy smell; meanwhile, the procyanidins in the grape seed extract, vitamin C and vitamin E form a "water-soluble + fat-soluble" synergistic antioxidant system, which can more comprehensively assist in scavenging free radicals, vitamin D3 can promote calcium absorption to assist bone health, and the bovine bone marrow microcapsule powder provides basic nutritional support for tissue maintenance, and the addition of the auxiliary materials also ensures the flowability and formability of the capsule contents, so that the proportioning of each component is accurate and stable, and finally the comprehensive effect of nutritional supplementation and functional assistance is realized.

[0013] Further, in the preparation of the bovine bone marrow microcapsule powder, the wall material includes denatured starch and malt dextrin in a weight ratio of (2-4):1, and the weight ratio of the wall material to the bone marrow oil is (1-3):1.

[0014] By adopting the technical scheme, the denatured starch and malt dextrin are compounded in a weight ratio of (2-4):1, which can not only exert the excellent emulsifying stability and embedding firmness of the denatured starch, but also can avoid the problems of loose embedding of single wall material and easy breakage or difficult dissolution of the wall material by virtue of the good water solubility and dispersibility of the malt dextrin; meanwhile, the weight ratio of the wall material to the bone marrow oil is (1-3):1, which can ensure that the bone marrow oil is fully wrapped to isolate oxygen and light and minimize the oxidation and deterioration of unsaturated fatty acids, and also will not dilute the proportion of effective components due to excessive wall material; the above ratio, in combination with the retention of heat-sensitive nutrients by vacuum drying at ≤60°C and the solvent residue-free advantage of supercritical CO2 extraction, makes the nutrients of the bovine bone marrow oil more stable and easily absorbed, and then cooperates with the "water-soluble + fat-soluble" antioxidant system of the grape seed extract and vitamins C and E, as well as the calcium absorption-promoting effect of vitamin D3, to finally realize the comprehensive effect of more complete nutrient retention, higher absorption efficiency and more optimal synergistic efficacy.

[0015] Further, the particle size D50 of the microcapsule powder is 10-50 μm, the bulk density is 0.35-0.55 g / mL, and the moisture content is ≤5%.

[0016] By adopting the above technical scheme, the particle size D50 is controlled in 10-50 μm, which can not only increase the contact area of the beef bone marrow oil and the intestinal mucosa through a larger specific surface area to improve the absorption efficiency of the nutritional ingredients, but also ensure that the powder has good fluidity to avoid problems such as caking and uneven metering during subsequent filling of the capsules; the bulk density is set to 0.35-0.55 g / mL, which is moderate to balance the filling property and looseness of the powder, so that the powder will not be too light to cause loss during filling, nor too heavy to cause compaction of the material in the capsule to affect disintegration and ingredient release; the moisture content is ≤5%, which can effectively inhibit the growth of microorganisms, while avoiding the softening of the microcapsule wall material due to moisture absorption, preventing the internal beef bone marrow oil from being oxidized and deteriorated due to the presence of moisture, and further prolonging the shelf life of the product. These parameters, combined with the previous wall material ratio, low-temperature drying and supercritical extraction process, and the synergistic effect of grape seed extract and various vitamins, ultimately achieve the comprehensive effect of more stable nutrient retention, more efficient absorption, and more controllable product quality.

[0017] Further, the beef bone marrow oil extraction method is as follows: the pretreated beef bone marrow is loaded into an extraction kettle, carbon dioxide is used as the extraction agent, and extraction is carried out under the conditions of a temperature of 35-45℃ and a pressure of 20-30 MPa for 2-3 h.

[0018] By adopting the above technical scheme, the setting of the supercritical CO2 extraction conditions can maximize the retention of the nutrients and activity of the beef bone marrow oil, thereby improving the overall efficacy of the capsule.

[0019] The extraction temperature is controlled in 35-45℃, which is in the appropriate temperature range of the supercritical state of CO2, and can avoid high-temperature damage to heat-sensitive components (such as unsaturated fatty acids and phospholipids) in the beef bone marrow oil, thereby retaining the nutritional value; the pressure of 20-30 MPa can make the supercritical CO2 have stronger solubility, which can efficiently extract the effective active lipids in the beef bone marrow oil, and at the same time, the high selectivity of supercritical extraction can reduce the mixing of impurities (such as proteins and cholesterol), thereby improving the purity of the beef bone marrow oil; the extraction time of 2-3 h achieves a balance between “sufficient extraction” and “high efficiency and energy saving”, which ensures the extraction rate of the beef bone marrow oil and avoids the residual of effective components, and also does not cause energy consumption increase or excessive extraction of irrelevant components due to too long time. The high-purity and high-activity beef bone marrow oil obtained by the extraction process, combined with subsequent microcapsule embedding and synergistic effect with grape seed extract and vitamins, ultimately makes the capsule have more advantages in nutrient retention, ingredient purity and efficacy exertion.

[0020] Further, the mass percentage of procyanidins in the grape seed extract is ≥95%.

[0021] By adopting the above technical scheme, proanthocyanidin is the key substance for grape seed to play an antioxidant role, and the high purity of ≥95% means that the effective component concentration in the unit weight of the extract is extremely high, so that free radicals in the body can be more efficiently captured and oxidative damage can be reduced under the given amount of capsules, and the problem of "dilution of effective components" caused by too high proportion of impurities (such as sugars and low-activity phenols) can be avoided; at the same time, high purity can minimize potential interference (such as affecting the absorption of other components and increasing the risk of discomfort) that impurities may cause, thereby improving product safety; in addition, high-purity proanthocyanidin has stronger synergy with vitamin C and vitamin E in the capsules, and the three can more accurately form a three-dimensional antioxidant system of "water-soluble (proanthocyanidin, vitamin C) + fat-soluble (vitamin E)", thereby further amplifying the effect of scavenging free radicals and protecting cells, and the content of effective components is stable and controllable, so that the antioxidant effect of each capsule can be ensured to be consistent.

[0022] Further, the preparation method of the grape seed extract is as follows: the enzymolyzed grape seeds are mixed with an ethanol solution with a volume fraction of 60-70% at a ratio of 1:(8-10), microwave extraction is performed, the microwave power is 300-500 W, the extraction time is 15-25 minutes, the extraction is performed at 40-50°C, after the extraction is completed, the ethanol is recovered by reduced pressure distillation, and the grape seed extract is obtained.

[0023] By adopting the above technical scheme, the preparation method of the grape seed extract can optimize the quality of components from the aspects of extraction efficiency, activity retention, and purity control, thereby strengthening the overall effect of the capsules.

[0024] The ethanol solution with a volume fraction of 60-70% is selected, the polarity of which is highly matched with the solubility of proanthocyanidin, which can not only efficiently dissolve and extract proanthocyanidin, but also reduce the dissolution of impurities such as polysaccharides and low-activity phenols, thereby laying a foundation for obtaining proanthocyanidin with a high purity of ≥95%; the solid-liquid ratio of 1:(8-10) can ensure that the grape seeds are in full contact with the ethanol, thereby avoiding incomplete dissolution of effective components due to insufficient solvent, while not excessively increasing the energy consumption of subsequent distillation, thereby balancing the extraction rate and economy.

[0025] The microwave extraction of 300-500 W for 15-25 minutes can quickly heat the inside of grape seed cells, destroy the cell wall structure, and accelerate the release of proanthocyanidin, thereby greatly shortening the time and improving the efficiency compared with traditional extraction; the extraction temperature of 40-50°C is at the balance point of "efficient dissolution" and "activity retention", which can assist the dissolution of proanthocyanidin by ethanol, and avoid the degradation and inactivation of proanthocyanidin caused by high temperature.

[0026] Finally, ethanol is recovered by reduced pressure distillation. The reduced pressure environment can reduce the boiling point of ethanol, achieve low-temperature alcohol removal, further reduce the damage of procyanidins caused by high temperature, and recycle ethanol for reuse, thereby reducing costs and pollution. Finally, the grape seed extract with high purity and high activity can more efficiently play an antioxidant role and improve the stability and safety of the capsule.

[0027] Further, the excipients include one or more of microcrystalline cellulose, silicon dioxide, and magnesium stearate.

[0028] By adopting the above technical solution, the microcrystalline cellulose has both filling and disintegrating properties, can serve as a “basic carrier” to make up for the weight of the capsule content and ensure uniform content of active ingredients in each capsule, and can promote the disintegration of the capsule shell in the body, accelerate the release and absorption of the bovine bone marrow microcapsule powder, grape seed extract, and vitamins; the silicon dioxide as an anti-caking agent can adsorb excess trace moisture in the powder, prevent the bovine bone marrow microcapsule powder, grape seed extract, and other ingredients from caking due to moisture absorption, ensure that the content is always loose, and avoid measurement deviation or capsule adhesion during filling; the magnesium stearate is an excellent lubricant that can reduce friction between the powder and the capsule filling equipment, ensure a smooth filling process, avoid powder leakage caused by capsule shell wear, and also help the ingredients to mix more evenly. The three excipients are used as needed to ensure the stability of capsule production and the integrity of the product form, and to clear obstacles for uniform release and efficient absorption of active ingredients, thereby indirectly improving the reliability of the overall efficacy of the capsule.

[0029] In a second aspect, the application provides a preparation method of a bovine bone marrow grape seed vitamin capsule, which adopts the following technical solution.

[0030] A preparation method of a bovine bone marrow grape seed vitamin capsule, comprising the following steps:

[0031] S1. Mixing: uniformly mixing bovine bone marrow microcapsule powder, grape seed extract, vitamin C, vitamin E, vitamin D3, and excipients;

[0032] S2. Filling: sieving the mixed material and filling it into a hollow capsule to obtain a bovine bone marrow grape seed vitamin capsule.

[0033] In summary, the application has the following beneficial effects:

[0034] The application uses modified starch and malt dextrin for microcapsule embedding, which not only isolates oxygen and light to prevent bone marrow oil from oxidizing and deteriorating, but also refines particles to improve intestinal absorption efficiency and masks fishy smell. Then, the bovine bone marrow microcapsule powder, grape seed extract, and vitamins are compounded. The procyanidins in the grape seed extract form a "water-soluble + fat-soluble" synergistic antioxidant system with vitamin C and vitamin E, which can more comprehensively assist in scavenging free radicals. Vitamin D3 can promote calcium absorption to assist bone health, and the bovine bone marrow microcapsule powder provides basic nutritional support for tissue maintenance. The addition of excipients also ensures the flowability and formability of the capsule contents, making the ingredient ratio precise and stable, and ultimately achieving the comprehensive effect of nutritional supplementation and functional assistance. DETAILED DESCRIPTION

[0035] The application will be further described in detail below in conjunction with examples.

[0036] Raw materials and intermediate preparation examples

[0037] Raw materials

[0038] It should be specially noted that: in the following examples, the specific conditions are not specified, and the routine conditions or the conditions recommended by the manufacturer are used. The raw materials used in the following examples can be obtained from ordinary market sources unless otherwise specified.

[0039] Preparation example

[0040] Preparation example 1

[0041] A bovine bone marrow microcapsule powder is prepared by the following method:

[0042] (1) The frozen bovine bone marrow is taken out from the -20℃ freezer and slowly thawed in the 4℃ refrigerator for 12-24 hours to avoid loss of nutrients due to rapid thawing. The thawed bovine bone marrow is sterilized by high-voltage pulse electric field sterilization equipment with an electric field strength of 30-40 kV / cm and a pulse frequency of 100-200 Hz for 5-10 minutes. Then, the fat is removed by ultrasonic wave assisted defatting equipment with an ultrasonic frequency of 20-40 kHz and a power of 300-500 W at 40-50℃ for 30-60 minutes to achieve a fat removal rate of more than 90%. Then, it is broken and vacuum dried at a temperature ≤60℃;

[0043] (2) The pretreated bovine bone marrow is loaded into an extraction kettle, and carbon dioxide is used as the extraction agent. The extraction is carried out at a temperature of 35-45℃ and a pressure of 20-30 MPa for 2-3 hours to obtain bovine bone marrow oil;

[0044] (3) 2.4 kg of modified starch hydroxypropyl distarch phosphate, 0.8 kg of maltodextrin, were added into 1.5 L of deionized water, and dissolved at 75 °C for 30 min with shearing at 3000 rpm to obtain a wall material solution;

[0045] 2.73 kg of bone marrow oil (60 °C) was poured into the wall material solution, and high-speed shearing was performed at 11000 rpm for 10 min to form a colostrum;

[0046] The stable emulsion was obtained by passing through the high-pressure homogenizer 40 MPa for 3 cycles;

[0047] Spray drying microencapsulation: the parameters of the spray dryer were as follows: inlet air temperature 170 °C, outlet air temperature 80 °C, feed rate 15 mL·min -1 , nozzle pressure 0.25 MPa, and the obtained bovine bone marrow microcapsule powder had a D50 of 28 μm, a bulk density of 0.46 g / mL, and a moisture content of 3.2 %.

[0048] Preparation Example 2

[0049] Different from Preparation Example 1, in Preparation Example 2, the modified starch hydroxypropyl distarch phosphate was 2.1 kg, and the maltodextrin was 1.1 kg, and the obtained bovine bone marrow microcapsule powder had a D50 of 32 μm, a bulk density of 0.43 g / mL, and a moisture content of 3.6 %.

[0050] Preparation Example 3

[0051] Different from Preparation Example 1, in Preparation Example 3, the modified starch hydroxypropyl distarch phosphate was 2.56 kg, and the maltodextrin was 0.64 kg, and the obtained bovine bone marrow microcapsule powder had a D50 of 22 μm, a bulk density of 0.51 g / mL, and a moisture content of 2.8 %.

[0052] Preparation Example 4

[0053] Different from Preparation Example 1, in Preparation Example 4, the modified starch hydroxypropyl distarch phosphate was 1.6 kg, and the maltodextrin was 1.6 kg, and the obtained bovine bone marrow microcapsule powder had a D50 of 37 μm, a bulk density of 0.39 g / mL, and a moisture content of 4.1 %.

[0054] Preparation Example 5

[0055] A grape seed extract was prepared by the following method:

[0056] 1) 5 kg of enzyme-treated grape seed powder was put into a microwave extraction tank;

[0057] 2) 45 L of 65% ethanol was added, and stirring was started at 60 rpm, and microwave heating was started synchronously;

[0058] 3) After the temperature rose to 45 °C, 400 W was continued to extract for 20 min;

[0059] 4) Immediately after extraction, filter with 0.45 μm plate frame, get about 42 L of clear filtrate;

[0060] 5) Distill ethanol at 45℃, -0.085 MPa, concentrate to 1 / 10 of the original volume;

[0061] 6) Purify the concentrated solution with AB-8 macroporous resin, flow rate 1 BV h -1 , first wash with 2 BV of water, then elute with 40% ethanol for 3 BV, collect the eluate;

[0062] 7) Concentrate again under reduced pressure, vacuum freeze-dry, get 0.87 kg of purple red grape seed extract.

[0063] Example

[0064] Example 1

[0065] A method for preparing a bovine bone marrow grape seed vitamin capsule is as follows:

[0066] S1. Mixing: according to Table 1, mix bovine bone marrow microcapsule powder, grape seed extract, vitamin C, vitamin E, vitamin D3, and excipients in a planetary mixer. Set the revolution speed of the planetary mixer to 100-150 r / min, set the rotation speed to 200-300 r / min, mix for 30-45 minutes to ensure that the components are fully mixed and uniform. During the mixing process, sample the material every 10 minutes, detect the content of each component by high performance liquid chromatograph, atomic absorption spectrometer, etc., and ensure that the content deviation of each component is controlled within ±5%;

[0067] S2. Filling: use a full-automatic capsule filling machine to fill the capsules. Before filling, strictly detect the quality of the capsule shells, including appearance inspection, no cracks, no deformation, uniform color, thickness measurement, and thickness deviation controlled within ±0.05 mm. Set the filling amount of the filling machine to 0.5 g per capsule, and the filling speed to 70 capsules per minute, to ensure that the weight difference of each capsule is controlled within ±3%. After filling, the capsules enter a vacuum drying oven for drying treatment, set the drying temperature to 45℃, control the vacuum degree to -0.09 MPa, and the drying time to 2.5 hours, so that the water content in the capsules is reduced to below 3%. Finally, polish the capsules by a capsule polishing machine to make the surface smooth, then perform appearance inspection and quality sampling inspection, and remove the capsules that are unqualified in appearance and content, to obtain the bovine bone marrow grape seed vitamin capsules.

[0068] Table 1 Raw material ratio table of Examples 1-3 (kg)

[0069]

[0070] Wherein, the bovine bone marrow microcapsule powder is from Preparation Example 1; the grape seed extract is from Preparation Example 5; and the excipients are microcrystalline cellulose, silicon dioxide and magnesium stearate with a weight ratio of 24:2:1.

[0071] Examples 4-6

[0072] Different from Example 2, the bovine bone marrow microcapsule powder in Examples 4-6 is from Preparation Examples 2-4, respectively.

[0073] Comparative Example

[0074] Comparative Example 1

[0075] Different from Example 1, the formulation is exactly the same as Example 1, and the only difference is that:

[0076] The bovine bone marrow oil is not microencapsulated, but directly mixed with the grape seed extract and vitamin powder in liquid form by adsorption;

[0077] The liquid bovine bone marrow oil, which is obtained from step (2) of Preparation Example 1 by supercritical CO2 extraction, is not microencapsulated in step (3); the liquid oil is sprayed and adsorbed onto the surface of an equal amount of microcrystalline cellulose with a nozzle diameter of 0.8 mm, an inlet air temperature of 50°C, and an adsorption rate of ≥95%), forming “oil-adsorbed particles”, which are then further mixed, filled, dried, and polished with the grape seed extract, vitamins, and the remaining excipients according to the method of Example 1.

[0078] Performance Testing

[0079] The products obtained from the examples and comparative examples are subjected to the following performance testing, and the test results are shown in Table 2.

[0080] The accelerated oxidation POV increment test is carried out according to the “Determination of Peroxide Value in Foods” GB 5009.227-2023, with a condition of 60°C for 7d.

[0081] Animal experiments are conducted on rats to examine the serum SOD activity and MDA content:

[0082] Serum SOD activity: xanthine-xanthine oxidase method, reagent kit: Nanjing Jiancheng A001-3, instrument: ultraviolet-visible spectrophotometer, 550 nm;

[0083] Take serum 20 μL, add reagent one 1.0 mL, reagent two 0.1 mL, reagent three 0.1 mL in turn, mix well; 37℃ water bath 20 min; add chromogenic agent 0.2 mL, stand for 10 min; measure absorbance at 550 nm (blank zero); calculate: SOD activity (U mL -1 ) = (Acontrol-Ameasurement) / Acontrol÷50 % × dilution factor. Results are expressed as mean ± SD, n=10.

[0084] The serum MDA content was determined by TBA colorimetry, reagent kit: Nanjing Jiancheng A003-2; instrument: ultraviolet-visible spectrophotometer, 532 nm;

[0085] Take serum 0.1 mL, add reagent one 0.1 mL, reagent two 0.2 mL, reagent three 0.2 mL, mix well;

[0086] 95℃ water bath 40 min, cool with cold water; centrifuge at 3 500 r / min -1 for 10 min; take supernatant 0.2 mL, measure absorbance at 532 nm; calculate: MDA content (nmol / m -1 L) = (Ameasurement-Ablank) / standard slope × dilution factor. Results are expressed as mean ± SD, n=10.

[0087] Table 2 Performance test results

[0088]

[0089] The POV increment of examples 1-6 is only 0.38-0.59 , which is 8.8-12.1 times lower than 4.60 of comparative example 1, indicating that microcapsule embedding significantly inhibits the generation of primary oxidation products of bovine bone marrow oil. Among them, example 2 performs best, and example 6 is the worst but still <0.6 , indicating that the proportion of wall material within the limited range of the application can effectively isolate oxygen, and the proportion of high denaturation starch is better.

[0090] The serum SOD activity of examples 1-6 is 238-268 U / mL -1 , which is 5.6-19.1 % higher than 225 U / mL -1 of comparative example 1; example 2 reaches a peak of 268 U / mL -1 , corresponding to the lowest POV increment, which reflects the reduction of oxidative stress and the recovery of endogenous antioxidant enzyme activity.

[0091] Serum MDA content: 3.8-5.1 nmol mL for Examples 1-6 -1 5.9 nmol mL for Comparative Example 1 -1 Decrease 13-36 %; Example 2 MDA lowest, with SOD peak forming a "high-eliminate low-lipid peroxidation" closed loop, verifying that the water-soluble plus lipid-soluble synergistic antioxidant system is effectively operating in vivo.

[0092] The specific embodiments are only an explanation of the present application, which is not a limitation to the present application, and the person skilled in the art can make modifications to the embodiments without creative contribution after reading the specification, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. A bovine bone marrow grape seed vitamin capsule comprising a hollow capsule and a capsule content, characterized in that, The capsule content comprises the following raw materials by weight percentage: 30-50% of bovine bone marrow microcapsule powder, 10-20% of grape seed extract, 5-10% of vitamin C, 2-5% of vitamin E, 0.01-0.05% of vitamin D3, and excipients to make up to 100%; The bovine bone marrow microcapsule powder is prepared by the following steps: (1) Fresh bovine bone marrow is washed and broken, and then vacuum dried at a temperature of ≤60℃; (2) The dried bovine bone marrow is used to extract bovine bone marrow oil by supercritical CO2 extraction technology; (3) The bovine bone marrow oil is subjected to microcapsule embedding treatment with modified starch and malt dextrin as wall materials to obtain bovine bone marrow microcapsule powder.

2. The bovine bone marrow grape seed vitamin capsule of claim 1, wherein in the preparation of the bovine bone marrow microcapsule powder, the wall material comprises modified starch and malt dextrin in a weight ratio of (2-4):1, and the weight ratio of the wall material to the bovine bone marrow oil is (1-3):

1.

3. The bovine bone marrow grape seed vitamin capsule of claim 1, wherein the particle size D50 of the microcapsule powder is 10-50μm, the bulk density is 0.35-0.55g / mL, and the moisture content is ≤5%.

4. The bovine bone marrow grape seed vitamin capsule of claim 1, wherein the bovine bone marrow oil extraction method is as follows: the pretreated bovine bone marrow is loaded into an extraction kettle, carbon dioxide is used as the extraction agent, and the extraction is carried out at a temperature of 35-45℃ and a pressure of 20-30MPa for 2-3h.

5. The bovine bone marrow and grape seed vitamin capsule according to claim 1, characterized in that, The mass percentage of procyanidins in the grape seed extract is ≥95%.

6. The bovine bone marrow grape seed vitamin capsule of claim 5, wherein the preparation method of the grape seed extract is as follows: the enzymolyzed grape seeds are mixed with an ethanol solution with a volume fraction of 60-70% at a ratio of 1:(8-10), microwave extraction is carried out at a microwave power of 300-500W for 15-25min at a temperature of 40-50℃, and after the extraction is completed, the ethanol is recovered by reduced pressure distillation to obtain the grape seed extract.

7. The bovine bone marrow grape seed vitamin capsule of claim 1, wherein the excipients include one or more of microcrystalline cellulose, silicon dioxide, and magnesium stearate.

8. A method of preparing the vitamin capsule of the grape seeds of the bovine bone marrow according to any one of claims 1-7, characterized by, The method comprises the following steps: S1. Mixing: uniformly mix the bovine bone marrow microcapsule powder, grape seed extract, vitamin C, vitamin E, vitamin D3, and excipients; S2. Filling: sieve the mixed materials and fill them into hollow capsules to obtain the bovine bone marrow grape seed vitamin capsule.

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