Plant seed oil composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a plant seed oil composition as well as a preparation method and application thereof. The plant seed oil composition is prepared from a component A, a component B and a component C. The component A is prepared from, by mass, 32-35 parts of isononyl isononanoate and 0.01-0.05 part of ormosia henryi bark extract, and the component C is prepared from, by mass, 1.8-2.2 parts of hydroxypinacolone retinoate, 1-1.5 parts of candida albicans fermented oil, 0.8-1.2 parts of prunus amygdalus dunn seed oil, 0.4-0.8 part of millettia indica extract and 1.5-2.5 parts of isosorbide dimethyl ether. And the component B comprises 55-65 parts of an oil phase solvent. The plant seed oil composition can be used for essence oil, and has the advantage of improving the permeation and absorption effects of the essence oil in cuticle.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, more particularly, it relates to a plant seed oil composition and a preparation method and application thereof. BACKGROUND

[0002] In recent years, the concept of "nourishing skin with oil" has gradually emerged, and essence oil as a new single product in the skin care market has been favored by many consumers due to its remarkable effect on improving dry skin, damaged barrier and wrinkles. Such products play a role by penetrating the skin through oil components, which meets the needs of modern consumers for efficient skin care and occupies an increasingly important position in the skin care market.

[0003] Skin has both hydrophilic and hydrophobic characteristics, and the outermost stratum corneum is composed of lipids and keratinocytes in a "brick wall structure", which is not only the key to maintaining skin moisture retention and barrier function, but also a natural hydrophobic barrier. Under this structure, oil-soluble components with larger molecules (such as long-chain fatty acids and macromolecular plant waxes) cannot penetrate the dense intercellular space of the stratum corneum, resulting in generally low absorption of essence oil. At the same time, the hydrophobic stratum corneum and the hydrophilic epidermis and dermis form a penetration barrier, further increasing the difficulty of effective absorption of conventional essence oil by the skin. Therefore, how to break through the limitation of skin barrier on oil absorption and improve the penetration and skin comfort of essence oil has become a technical problem to be solved in the current field. SUMMARY

[0004] In order to improve the penetration and absorption of essence oil in the stratum corneum, the present application provides a plant seed oil composition and a preparation method and application thereof.

[0005] The plant seed oil composition provided by the present application adopts the following technical scheme: A plant seed oil composition, comprising component A, component B and component C, wherein the component A comprises 32-35 parts by mass of isononyl isononanoate and 0.01-0.05 parts by mass of peruvia bark extract, the component C comprises 1.8-2.2 parts by mass of hydroxyl pinacolone retinol, 1-1.5 parts by mass of manuka honey yeast fermentation oil, 0.8-1.2 parts by mass of sweet almond seed oil, 0.4-0.8 parts by mass of helichrysum extract, 1.5-2.5 parts by mass of isosorbide dimethyl ether, and the component B comprises 55-65 parts by mass of oil phase solvent.

[0006] By adopting the above technical scheme, in component A, isononyl isononanoate is used as a low-polarity light oil, which has excellent skin spreading and penetrating properties, can quickly penetrate the stratum corneum and carry other components into the skin as a carrier, has high compatibility with stratum corneum lipids, and can reduce the penetration resistance; the extract of pernambuco tree bark plays a soothing and repairing effect, and contains flavonoids and polyphenol active substances, which can inhibit the release of inflammatory factors (such as IL-6 and TNF-α), relieve the irritation caused by component penetration, promote the synthesis of stratum corneum ceramides, maintain the integrity of intercellular lipids, reduce trans-epidermal water loss (TEWL), create a stable and moisturizing skin microenvironment for subsequent component absorption, and reduce the incidence of redness and pain on dry and sensitive skin. The oil phase solvent in component B accounts for 55-65 parts, which is used as a basic dispersion medium. If it is of plant origin (such as coconut oil derivatives and light olive oil), it contains unsaturated fatty acids such as oleic acid and linoleic acid, which can form lipid complexes with cholesterol and ceramides in the stratum corneum, not only enhancing the affinity with the stratum corneum lipid structure, but also softening the stratum corneum, promoting the orderly arrangement of keratinocytes rather than destroying the stratum corneum, reducing the barrier effect of the skin barrier on oil components, and supplementing the basic lipids of the skin to avoid excessive barrier exfoliation during penetration, thereby providing a mild and safe channel for the penetration of components A and C. In component C, the components synergistically enhance the penetration effect and skin care effect: isosorbide dimethyl ether as a penetration enhancer can destroy the orderly arrangement of stratum corneum lipids, expand the intercellular space of keratinocytes, and promote the penetration of oil-soluble components; in addition to being rich in unsaturated fatty acids and small molecule active substances, the Candida bombicola ferment oil also contains small molecule peptides and amino acids produced during fermentation, which can penetrate into the superficial dermis, promote skin microcirculation, and improve dull skin tone. At the same time, the unsaturated fatty acids can inhibit the activity of stratum corneum serine proteases, reduce the excessive shedding of keratinocytes, and maintain the appropriate thickness of the stratum corneum; the molecular structure of sweet almond seed oil is small and similar to skin lipids, which is easily absorbed by the stratum corneum, and contains vitamin E (tocopherol) that can scavenge free radicals, protect the skin from oxidative damage, and vitamin B (such as panthenol) that can promote skin cell repair and improve small cracks and desquamation caused by dryness, while reducing the roughness of the skin and improving the smoothness of the skin during the process of supplementing nutrients; under the action of the penetration enhancer system, hydroxyl pinacolone retinoate can more efficiently play an anti-aging and repairing role; the spilanthes extract further relieves inflammation, and contains apigenin and luteolin that can inhibit the activity of cyclooxygenase (COX-2) and reduce the generation of prostaglandin E2 (PGE2), relieve skin redness and pain caused by high-penetration components, regulate the immune response of the skin, reduce the frequency of redness on sensitive skin, and reduce skin discomfort.

[0007] In addition, the proportions of the components are optimized to avoid the sticky feeling caused by high concentration of oil, and the combination of isononyl isononanoate and oil phase solvent makes the composition light in texture, and the skin feels fresh after absorption, solving the pain points of traditional essence oil "poor absorption, heavy skin feeling", realizing the balance of high efficient penetration and comfortable use, and effectively solving the problem of skin barrier limiting oil absorption.

[0008] Optionally, the component B further comprises 0.4-0.6 parts of white pool seed oil, 0.3-0.5 parts of ascorbic acid tetraisopalmitate, 0.2-0.4 parts of silybum marianum seed oil, 0.1-0.15 parts of chicken fruit seed oil, 0.1-0.18 parts of myrobalan leaf / stem extract, and 0.01-0.02 parts of sea fennel extract.

[0009] By adopting the above technical scheme, a variety of plant active ingredients are added in the base oil phase solvent to form a composite protection network of "antioxidation-barrier repair-soothing". The white pool flower seed oil contains more than 98% long-chain fatty acids (C20-C24, such as arachidonic acid, behenic acid), and the matching degree of the molecular chain length with the lipid chain length of the stratum corneum is as high as more than 85%, which is highly compatible with the lipid of the stratum corneum. It not only can enhance the barrier water-locking function - it can reduce the skin water evaporation rate by 20% to 30% in a dry environment, but also can form a stable lipid film to inhibit lipid peroxidation and repair the barrier damage caused by ultraviolet rays, prolong the barrier protection time; as a lipid-soluble vitamin C derivative, ascorbyl tetraisopalmitate gradually releases active VC after penetrating into the epidermis, inhibits tyrosinase activity and scavenges free radicals; in addition to inhibiting the NF-κB pathway to reduce skin inflammatory response, silymarin in silybum marianum seed oil can also scavenge lipid peroxides (such as malondialdehyde MDA) and neutralize the small amount of reactive oxygen species (ROS) generated when the active oxygen component (hydroxyl pinacolone retinoate) penetrates, while protecting the epidermal basal layer cells, promoting normal cell differentiation, and avoiding abnormal thickening of the stratum corneum; chicken egg fruit seed oil is rich in Omega-6 fatty acids (mainly linoleic acid), which is an essential fatty acid for the skin (the human body cannot synthesize itself) and can directly participate in the synthesis of stratum corneum lipids, increase the content of ceramide 1 and ceramide 3, regulate the water and oil balance of the skin, improve dryness and desquamation, and at the same time enhance the skin's resistance to external pollutants (such as PM2.5), reduce inflammation caused by pollutants entering the deep layer of the skin; terpenoids (such as triterpenoid saponins) in myrobalan leaf extract can activate fibroblast proliferation and promote the synthesis of collagen III and elastin, not only increasing skin elasticity and reducing fine lines, but also repairing minor damage to the dermis layer (such as water loss in the dermis layer caused by dryness), while regulating the skin pH value to a healthy range of about 5.5, inhibiting the growth of harmful bacteria such as Propionibacterium acnes, and preventing acne; as a marine plant active substance, sea fennel extract not only stimulates keratinocyte renewal, but also contains marine polysaccharides and betaine to provide nutrients for keratinocytes, speed up the migration cycle of cells from the basal layer to the stratum corneum, and promote the release of lipids from the stratum corneum, helping to build a complete lipid barrier. The above-mentioned components form a synergistic system with the base oil phase, not only enhancing the antioxidant capacity of the composition, but also supplementing essential fatty acids for the skin, regulating inflammatory signaling pathways, and reducing the irritability of active ingredients (such as retinoids), especially suitable for sensitive skin and barrier-damaged people.

[0010] Optionally, the fermentation oil of the Trichosporon meihechi includes, by mass percentage, Trichosporon meihechi / glucose / rapeseed oil acid methyl ester fermentation product 38% to 42%, olea europaea fruit oil 15% to 18%, sunflower seed oil 10% to 12%, angelica gigantea root extract 5% to 7%, lithospermum root extract 3.5% to 4.5%, glycyrrhiza root extract 2% to 3%, and the balance being acacia rigida nut oil.

[0011] By adopting the above technical solution, the fermentation broth of Trichosporon meihechi / glucose / rapeseed oil acid methyl ester is rich in short-chain fatty acids (such as propionic acid and butyric acid), which can regulate the skin microecological balance and inhibit the reproduction of Propionibacterium acnes; olea europaea fruit oil and sunflower seed oil provide essential fatty acids (linoleic acid and α-linolenic acid) to repair the intercellular lipid "brick wall structure"; ferulic acid in angelica gigantea root extract has an anti-ultraviolet damage effect, and shikonin in lithospermum root extract has an anti-inflammatory soothing effect, and dipotassium glycyrrhizinate in glycyrrhiza root extract inhibits histamine release, all of which synergistically reduce skin redness and sensitivity; acacia rigida nut oil, as "liquid gold", is rich in vitamin E and phytosterols, and has antioxidant and wound healing promoting effects. The fermentation oil enhances the penetration of the essence oil while solving the problems of acne and irritation that may be caused by traditional oil products, and is especially suitable for people with oily skin.

[0012] Optionally, the Trichosporon meihechi fermentation oil also needs to be treated as follows: The Trichosporon meihechi fermentation oil is preheated at 40-45°C and filtered; The filtered fermentation oil is exposed to a low-temperature plasma field generated by dielectric barrier discharge (DBD) in the form of a film with a thickness of ≤2mm for treatment; The treatment power is 40-60W, the treatment time is 5-10 minutes, and the treatment atmosphere is an inert gas; The treated fermentation oil is cooled to room temperature under helium gas protection.

[0013] By adopting the technical scheme, the main technical effect is to realize physical activation and extreme stabilization treatment of the Candida bombicola fermentation oil. In a pure inert gas atmosphere, the low-temperature plasma field is mainly composed of high-energy electrons, ions and excited inert gas molecules, and the effect on oil is mainly physical bombardment and energy transfer. This treatment can effectively break the interaction of part of the macromolecular micelles or weak aggregates in the fermentation oil, significantly reduce the system viscosity, and improve its spreading and permeability, thereby optimizing the skin feel of the final essence oil product. At the same time, the high-energy particle flow can realize in-situ sterilization at room temperature and high efficiency, completely kill the microorganisms that may exist in the fermentation oil, and does not need to introduce any chemical preservative, greatly guarantees the biological safety of the product. The whole process is completed at low temperature, completely avoids the risk of inactivation of heat-sensitive active ingredients due to thermal degradation, and maximizes the retention of the full biological activity of the fermentation oil. In addition, the treatment is carried out under inert gas protection, which eliminates the occurrence of oxidation reaction.

[0014] Optionally, the helium gas is doped with oxygen, and the volume ratio of the oxygen is 20% to 30%.

[0015] By adopting the above technical scheme, after introducing 20% to 30% of oxygen, a large number of high-reactivity oxygen species (ROS) will be generated in the plasma field. These active particles can cause controlled slight oxidation of unsaturated fatty acids in the oil, mainly generating primary oxidation products-lipid hydroperoxides (LOOH). Recent research shows that lipid hydroperoxides with specific structures and their decomposition products do not only have negative effects, but also can act as effective cell signaling molecules to activate the Nrf2 antioxidant defense pathway of skin cells, thereby up-regulating the synthesis of endogenous antioxidants such as superoxide dismutase (SOD) and glutathione peroxidase, and giving the product the effect of "biological activation". This "pre-oxidation" treatment consumes easily reactive components in the environment, and instead improves the oxidative stability of the subsequent storage of the oil.

[0016] Optionally, the hydroxyl pinakon retinoate is coated in a phospholipid-chitosan composite vesicle.

[0017] By adopting the technical scheme, the hydroxyl pinacolyl retinoate is coated in the phospholipid-chitosan composite vesicle, and the stability and irritation of the retinoid component are solved. The lipid bilayer formed by the hydrogenated soybean phospholipid and cholesterol can isolate the HPR from the contact with oxygen and light, so that the storage time of the HPR under the condition of 40 DEG C / 60%RH is significantly increased; the chitosan (degree of deacetylation ≥95%) has a positive charge, and the skin stratum corneum cells have a negative charge, so that the adsorption is enhanced by the electrostatic effect, the residence time of the vesicle on the skin surface is prolonged, the core-shell structure of the composite vesicle (the liposome core encapsulates the HPR, and the chitosan outer layer is crosslinked) forms a slow-release system, and the skin irritation (such as redness and desquamation) caused by high concentration impact is avoided. The wrapping technology improves the transdermal absorption rate of the HPR, and greatly reduces the irritation, so that the sensitive skin can be used.

[0018] Optionally, the specific method for coating the hydroxyl pinacolyl retinoate in the phospholipid-chitosan composite vesicle is as follows: The hydrogenated soybean phospholipid, cholesterol and hydroxyl pinacolyl retinoate are dissolved in anhydrous ethanol according to the mass ratio (7.5-8.5):2:1, and an oil phase liquid is formed in a water bath at 50-55 DEG C; The chitosan with a degree of deacetylation ≥95% is dissolved in a 0.3% lactic acid aqueous solution with a pH of 4.0-4.5, and the chitosan concentration is controlled to be 1.5-2.0 wt%, so as to obtain an aqueous phase liquid; The oil phase liquid and the aqueous phase liquid are mixed under a shear rate of 10 4 s-1 to form a pre-vesicle with a particle size of 80-120 nm; The pre-vesicle system is added with a 0.4%-0.6% sodium tripolyphosphate aqueous solution, the mass ratio of chitosan to sodium tripolyphosphate is controlled to be 5:1, and the stirring reaction is performed at 25-28 DEG C for 30-50 min under the condition of pH 5.0-5.5.

[0019] By adopting the technical scheme, the hydrogenated soybean phospholipid is completely melted to form a liquid crystal phase in the water bath at 50-55 DEG C, and the cholesterol together forms a stable lipid bilayer, so that the HPR encapsulation rate is high; 4 The high shear rate of 10 s-1 promotes the oil phase liquid droplets to be refined to the nanoscale to form a pre-vesicle with a particle size of 80-120 nm, the particle size range can penetrate through the intercellular channels of the stratum corneum cells; when the sodium tripolyphosphate and the chitosan are crosslinked according to the mass ratio of 5:1, a polyelectrolyte layer with a thickness of about 5 nm is formed on the surface of the vesicle, the electrostatic repulsion between the vesicles is enhanced, and aggregation is prevented. The obtained composite vesicle has good skin compatibility, and realizes the dual advantages of “high-efficiency penetration + long-acting slow release”.

[0020] In a second aspect, the application provides a preparation method of a plant seed oil composition, which adopts the following technical scheme: A preparation method of a plant seed oil composition, comprising the following steps: The component A is added into a container, heated to 85-90℃ to make it dissolve and disperse, cooled to 45-50℃, then the component B and the component C are added in sequence and dispersed to be free of particles, cooled to 38℃, to obtain the plant seed oil composition.

[0021] In a third aspect, the present application provides a plant seed oil composition and application thereof, which adopts the technical scheme as follows: The application of a plant seed oil composition to an essence oil.

[0022] To sum up, the present application has the following beneficial effects: 1. The present application significantly improves the transdermal delivery efficiency of active ingredients by using a multi-level synergistic penetration system composed of isononyl isononanoate, isosorbide dimethyl ether and nanovesicle technology. The system first softens the stratum corneum by using the excellent carrier property and compatibility with sebum of isononyl isononanoate; then expands the penetration channel by temporarily disturbing the ordered arrangement of stratum corneum lipids through isosorbide dimethyl ether; finally, the efficacy core ingredient hydroxyl retinol is accurately delivered by the electrostatic adsorption and nanometer size effect of phospholipid-chitosan composite nanovesicles, solving the technical bottleneck of low absorption rate of traditional essence oil.

[0023] 2. In the present application, low-temperature plasma treatment technology is preferably used to sterilize the Candida bombicola fermentation oil at room temperature, while realizing the biological activation and physical modification of its function. Treatment in an inert atmosphere can physically reduce the viscosity, improve the skin feel and extremely retain the activity; while in a specific proportion of helium-oxygen mixed atmosphere, it can induce the generation of lipid oxidation products with signal molecule function, and activate the skin's own antioxidant defense mechanism.

[0024] 3. In the present application, a plurality of refined plant oils and extracts are preferably compounded, and advanced coating and processing technology is used to realize efficient anti-aging while ensuring excellent stability, low irritation and multiple repair effects of the composition. White pool seed oil, milk thistle seed oil, etc. provide barrier repair and anti-inflammatory foundation; ascorbic acid tetraisopalmitate provides antioxidant protection; plasma-treated fermentation oil and coated retinol derivatives play a role from the aspects of microecological balance and cell regulation, respectively. The synergistic effects of various components complement each other, and finally form a high-performance essence oil product with strong penetration, refreshing skin feel, comprehensive efficacy and applicability to various skin conditions. DETAILED DESCRIPTION

[0025] The present application is further described in detail below in conjunction with examples. It is particularly pointed out that: in the following examples, the specific conditions are not specified, and the conventional conditions or the conditions recommended by the manufacturer are used. In the following examples, the raw materials used can be obtained from ordinary market sources unless otherwise specified.

[0026] Preparation Example of Fermentation Oil of Candida Beeiana Preparation Example 1 Prepare the following raw materials: Candida Beeiana Strain: Strain No. B80544, Collection Center No. ATCC22214.

[0027] Glucose: Food grade. Purity ≥ 99.5% (dry basis), loss on drying ≤ 0.5%, sulfate ash ≤ 0.05%.

[0028] Rapeseed oil methyl ester: Oleic acid (C18:1) content 50-65%, linoleic acid (C18:2) content 18-25%, linolenic acid (C18:3) content 6-12%. Methyl esterification rate ≥ 96.5% (GC method). Acid value: ≤ 0.5 mgKOH / g. Peroxide value: ≤ 5.0 mmol / kg. Iodine value: 95-115 gI2 / 100g.

[0029] Yeast extract: Total nitrogen ≥ 9.0%, amino nitrogen ≥ 4.0%.

[0030] Soybean peptone: Total nitrogen ≥ 13.0%, ash ≤ 8.0%, loss on drying ≤ 7.0%.

[0031] Anhydrous sodium sulfate: Analytical pure, purity ≥ 99.0%, loss on drying ≤ 0.5%.

[0032] Acacia nilotica kernel oil: Acid value: ≤ 2.0 mgKOH / g. Peroxide value: ≤ 10.0 mmol / kg. Saponification value 180-200 mgKOH / g. Main fatty acids: Oleic acid (40-55%), linoleic acid (25-35%). Natural tocopherol content: ≥ 800 mg / kg.

[0033] Olive fruit oil: Extra virgin standard. Acid value: ≤ 1.5 mgKOH / g. Peroxide value: ≤ 10.0 mmol / kg. Sterol composition: β-sitosterol accounts for ≥ 90% of total sterols.

[0034] Sunflower seed oil: High oleic type. Oleic acid content ≥ 75% of total fatty acids. Acid value ≤ 0.5 mgKOH / g. Peroxide value ≤ 5.0 mmol / kg.

[0035] Angelica acutiloba root extract: Total coumarin content ≥ 2.0% (HPLC method).

[0036] Lithospermum root extract: Total content of shikonin and derivatives ≥ 2.5% (HPLC method, calculated as shikonin).

[0037] Glycyrrhiza root extract: Glycyrrhizic acid content ≥ 10.0% (HPLC method).

[0038] Water: Purified water standard (conductivity < 5 μS / cm @ 25°C).

[0039] Preparation process: First step: Under sterile conditions, preserved Trichosporon asahii inoculate to fresh PDA slant medium, placed in 28°C thermostat incubator for 48 hours.

[0040] Primary seed liquid: pick full single colony from the slope, inoculate into 500ml conical flask containing 100ml seed medium, medium glucose 20g / L, yeast extract 10g / L, peptone 20g / L, pH natural. Placed in 28°C, 180rpm shaker for 24 hours, until the turbidity (OD600) of the culture reaches 2.0-3.0.

[0041] Secondary seed liquid: according to 10% inoculation, the primary seed liquid is transferred to a 5L conical flask containing 1L of the same seed medium. Continue to cultivate under the same conditions (28°C, 180rpm) for 18-20 hours, until OD600 reaches 5.0-6.0, as high-density, high-activity production seed liquid.

[0042] Second step: Prepare the fermentation medium in the fermenter. The formula is as follows (based on the final fermentation liquid volume): Glucose 50g / L, rapeseed oil acid methyl ester 30g / L, yeast extract 10g / L, soybean peptone 10g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.5g / L, defoaming agent: 0.1g / L, constant volume with deionized water to the required volume. Sterilize the fermenter at 121°C for 30 minutes, then cool to 28°C.

[0043] Inoculate the secondary seed liquid into the fermenter at 12% inoculation. Set the initial process parameters: Temperature: 28.0±0.5°C; Stirring speed: 300rpm; Ventilation: 1.0vvm (volume ratio); Tank pressure: 0.03-0.05MPa; pH: initially unadjusted, during fermentation, control the pH at 5.8-6.2 by automatically adding 2M NaOH solution.

[0044] In the early stage of fermentation, the dissolved oxygen will rapidly decrease. Through interlocking control, increase the stirring speed to 400rpm to maintain the dissolved oxygen saturation above 30%. In the middle and late stages, the speed can be gradually reduced as the cell metabolism slows down.

[0045] When the fermentation proceeds to about 36 hours, the residual sugar concentration is less than 10 g / L, the feeding pump is started to add glucose solution with a concentration of 400 g / L at a constant rate until the end of the fermentation, and the total amount of added sugar is about 30% of the initial sugar amount.

[0046] The total fermentation time is 108-120 hours. When at least two of the following three conditions are met, the fermentation is terminated: a) the dissolved oxygen value continues to rise and stabilizes at a high level; b) the wet weight of the bacteria no longer increases; c) the pH value tends to be stable.

[0047] Step 3: After the fermentation is completed, the fermentation broth is heated to 80°C for 30 minutes to inactivate the microorganisms and promote oil-water separation.

[0048] The inactivated fermentation broth is pumped into a disc centrifuge and centrifuged at a speed of 8000 rpm. This step separates the mixture into three layers: the upper layer is a light phase rich in target oil (mainly fermented oil and unused rapeseed oil methyl ester), the middle layer is an aqueous phase, and the bottom layer is bacterial residue.

[0049] The bottom layer of bacterial cells is collected and resuspended with 2 times its weight of phosphate buffer (pH 7.0). A high-pressure homogenizer is used to homogenize the cells 3 times at a pressure of 800 bar to break the cell wall and release the intracellular oil. The homogenized slurry is centrifuged again to collect the upper oil layer.

[0050] The upper light phase from the first centrifugation is combined with the oil phase extracted from the bacterial cells. Anhydrous sodium sulfate is added to the combined oil at a weight of 2%, and the mixture is stirred slowly for 30 minutes to absorb trace amounts of water.

[0051] First, the mixture is filtered through a plate and frame filter, and then filtered through 1 μm and 0.45 μm precision filter cartridges in sequence to obtain a clear, golden yellow refined Candida bombicola / glycerol / rapeseed oil methyl ester fermentation product. Samples are taken for testing of acid value, peroxide value, and major fatty acid composition.

[0052] Step 4: The cleanable and sterilizable heated stirred reaction kettle is cleaned and sterilized. High-purity nitrogen gas is introduced into the kettle for 10 minutes to replace the internal air and maintain a slightly positive pressure nitrogen atmosphere.

[0053] Under the protection of nitrogen, the oil phase ingredients are added to the reaction kettle in the following mass percentages: Styrax benzoin kernel oil 200 g, olive fruit oil 165 g, sunflower seed oil 110 g, and the stirring is started at 60 rpm and heating is started to slowly warm the oil phase mixture to 40±2°C, and the temperature is maintained for 15 minutes.

[0054] Maintain temperature and nitrogen atmosphere, add 400 g of Candida bombicola / glucose / rapeseed fatty acid methyl ester fermentation product, when adding, inject through the kettle side feed port in a fine stream while increasing the stirring speed to 200 rpm.

[0055] Add the active extracts one after another, each after being well dispersed before adding the next: Angelica acutiloba root extract 60 g, Arnebia root extract 40 g, Glycyrrhiza root extract 25 g.

[0056] When adding the extracts, briefly increase the stirring speed to 500-800 rpm for 5-10 minutes to ensure that these viscous or solid extracts are completely dispersed and dissolved, without visible particles.

[0057] Fifth step: After all the materials have been added, return the stirring speed to 200 rpm and continue stirring at 40°C for 60 minutes to allow the entire system to mix and mature.

[0058] Start the high-shear homogenizing head attached to the reactor and perform a 10-minute homogenization at 4000 rpm. Reduce the temperature of the well-mixed fermentation oil to 25-30°C. Under continued nitrogen protection, pump the oil into the filtration system and pass it through 1 μιη and 0.45 μιη polytetrafluoroethylene (PTFE) filter cartridges in sequence. The filtered Candida bombicola fermentation oil is obtained.

[0059] Further, the method described in the preparation example of the Candida bombicola fermentation oil is only one of the processes demonstrated in the present application, and in actual production, the raw material components can be reduced or the amount adjusted according to the steps described in the preparation example.

[0060] Treatment preparation example of Candida bombicola fermentation oil Preparation example 2 A treatment method for a Candida bombicola fermentation oil: Take 1000 g of the Candida bombicola fermentation oil prepared in preparation example 1 in a glass beaker, place it in a constant-temperature water bath at 45°C, and preheat for 20 minutes with a magnetic stirrer at 300 rpm to reduce the viscosity of the oil. Then, filter the preheated fermentation oil using a 100-mesh stainless steel filter screen.

[0061] Take 50 g of the filtered fermentation oil described above, pour it into a clean quartz sample dish, and ensure that the oil film is 2.0 mm thick. Place the quartz dish containing the oil sample in the center of the reaction chamber of the DBD plasma device. After closing the chamber, start the vacuum pump and pump the pressure in the reaction chamber to below 10 Pa to completely remove air. Then, pass helium into the chamber through a mass flow meter to normal pressure, pump the vacuum again and recharge the helium, and repeat this cycle 3 times to ensure that the atmosphere in the chamber is completely replaced by inert gas.

[0062] Helium gas was continuously introduced into the reaction chamber at a flow rate of 1.5 L / min to maintain a slight positive pressure in the chamber. The radio frequency power supply of the DBD plasma generator was started, and the processing power was set to 50 W. The sample was subjected to low-temperature plasma treatment for 8 minutes.

[0063] After the treatment was completed, the plasma power supply was turned off. The sample was taken out together with the quartz boat under the protection of continuous introduction of inert gas and immediately transferred to a glove box filled with high-purity nitrogen. The sample was allowed to cool to room temperature of 25°C under a nitrogen atmosphere. After cooling, the treated Candida bombicola fermentation oil was taken out of the quartz boat, packed into a light-tight brown glass bottle, and filled with nitrogen to displace the top air. The bottle was sealed and stored for later use.

[0064] Preparation Example 3 A method for treating a Candida bombicola fermentation oil: different from Preparation Example 1 in that the processing power was 40 W.

[0065] Preparation Example 4 A method for treating a Candida bombicola fermentation oil: different from Preparation Example 1 in that the processing power was 60 W.

[0066] Preparation Example 5 A method for treating a Candida bombicola fermentation oil: different from Preparation Example 1 in that the oil film thickness was 1.0 mm.

[0067] Preparation Example 6 A method for treating a Candida bombicola fermentation oil: different from Preparation Example 1 in that the oil film thickness was 3.0 mm.

[0068] Preparation Example 7 A method for treating a Candida bombicola fermentation oil: different from Preparation Example 1 in that helium gas was replaced by a mixture of helium gas and oxygen gas, wherein the volume fraction of the helium gas was 75% and the volume fraction of the oxygen gas was 25%.

[0069] Preparation Example of hydroxypinacolone retinoate coated in phospholipid-chitosan complex vesicles Preparation Example 8 A method for preparing hydroxypinacolone retinoate coated in phospholipid-chitosan complex vesicles: (1) Prepare the following raw materials: hydroxypinacolone retinoate (purity ≥ 98%), hydrogenated soybean phospholipid (hydrogenation degree ≥ 95%, phase transition temperature 55°C), cholesterol (purity ≥ 99%), chitosan (degree of deacetylation ≥ 95%, molecular weight 50-100 kDa), lactic acid (food grade, 85% aqueous solution), sodium tripolyphosphate (analytical pure), anhydrous ethanol (chromatographically pure), ultrapure water (resistivity ≥ 18.2 MΩ·cm). (2) Oil phase liquid preparation Weigh 4.0 g of hydrogenated soybean phospholipid, 1.0 g of cholesterol, and 500 mg of hydroxypinacolone retinoate into a tomato-shaped bottle.

[0070] Add 50 mL of anhydrous ethanol, and magnetically stir at 600 rpm in a 55°C water bath until completely dissolved to obtain a clear oil phase liquid, which is kept warm for use.

[0071] (3) Preparation of the water phase liquid Measure 500 mL of ultrapure water, add 1.5 mL of 85% lactic acid, and adjust the pH to 4.2 to prepare a 0.3% lactic acid aqueous solution.

[0072] Add 0.9 g of chitosan, and stir at 800 rpm in a 45°C water bath for 2 hours to obtain a transparent viscous water phase liquid, which is filtered through a 0.45 μm filter membrane to remove impurities.

[0073] (4) Prevesicle formation Uniformly inject the oil phase liquid into the 55°C water phase liquid, and simultaneously perform high-speed shearing mixing at a shearing rate of 10 4 s⁻¹.

[0074] Continue shearing for 20 minutes to obtain a milky prevesicle dispersion liquid (particle size detection: Malvern Zetasizer shows 102±8 nm, PDI<0.2).

[0075] (5) Chitosan-TPP crosslinking and solidification Prepare a 0.5% TPP aqueous solution: dissolve 0.18 g of TPP in 36 mL of ultrapure water.

[0076] Drop the TPP solution into the prevesicle system at a rate of 0.5 mL / min (chitosan:TPP mass ratio=5:1).

[0077] Control the pH to be 5.3 (fine-tune with 0.1 M lactic acid / NaOH), the temperature to be 27±1°C, and magnetically stir (300 rpm) for 40 minutes.

[0078] (6) Purification and product processing Transfer the reaction liquid to a tangential flow filtration system, and dialyze against 3 volumes of pH 5.5 phosphate buffer to remove free drugs, ethanol, and ionic impurities.

[0079] Preparation Example 9 A method for coating hydroxypinacolone retinoate in phospholipid-chitosan complex vesicles: the difference from Preparation Example 8 is that the hydrogenated soybean phospholipid, cholesterol, and hydroxypinacolone retinoate are dissolved in anhydrous ethanol according to a mass ratio of 7.5:2:1.

[0080] Preparation Example 10 A preparation method of encapsulating hydroxyl retinol in phospholipid-chitosan complex vesicles: the difference from preparation example 8 is that hydrogenated soybean phospholipid, cholesterol, and hydroxyl retinol are dissolved in anhydrous ethanol at a mass ratio of 8.5:2:1.

[0081] Example 1 A preparation method of a plant seed oil composition: Add 33.5 g of isononyl isononanoate and 0.03 g of frake bark extract into a reaction kettle, heat to 87±1℃, and stir until completely dissolved and dispersed to obtain component A.

[0082] Cool the system to 48±1℃, mix 35 g of coco-caprylate / caprate, 25 g of caprylic / capric triglyceride as component B, mix 2.0 g of hydroxyl retinol, 1.3 g of candida bombicola ferment oil, 1.0 g of sweet almond oil, 0.6 g of spilanthes extract, and 2.0 g of dimethyl isosorbide as component C, and then add components B and C into component A in sequence, mix, mechanically stir at 2000 rpm for 30 minutes, until the system is a uniform transparent liquid and no visible particles are present.

[0083] Cool to 38℃, filter through a 100 mesh screen to obtain the plant seed oil composition.

[0084] The candida bombicola ferment oil is prepared by the method described in preparation example 1, and in this example, the candida bombicola ferment oil includes 40% candida bombicola / glucose / methyl oleate fermentation product and 60% styrax argentea seed oil by mass percentage.

[0085] Example 2 A preparation method of a plant seed oil composition: the difference from example 1 is that component A includes 32 g of isononyl isononanoate and 0.01 g of frake bark extract, component C includes 1.8 g of hydroxyl retinol, 1 g of candida bombicola ferment oil, 0.8 g of sweet almond oil, 0.4 g of spilanthes extract, and 1.5 g of dimethyl isosorbide, and component B includes 38 g of coco-caprylate / caprate and 17 g of caprylic / capric triglyceride.

[0086] Example 3 A preparation method of a plant seed oil composition: the difference from example 1 is that component A includes 35 g of isononyl isononanoate and 0.05 g of frake bark extract, component C includes 2.2 g of hydroxyl retinol, 1.5 g of candida bombicola ferment oil, 1.2 g of sweet almond oil, 0.8 g of spilanthes extract, and 2.5 g of dimethyl isosorbide, and component B includes 43 g of coco-caprylate / caprate and 22 g of caprylic / capric triglyceride.

[0087] Example 4 A method for preparing a plant seed oil composition: The difference from Example 1 is that component B further includes 0.5g meadowfoam seed oil, 0.4g ascorbate tetraisopalmitate, 0.3g milk thistle seed oil, 0.12g passion fruit seed oil, 0.15g myrothamnus flabellifolia leaf / stem extract, and 0.015g sea fennel extract.

[0088] Example 5 A method for preparing a plant seed oil composition: The difference from Example 4 is that component B further includes 0.4g meadowfoam seed oil, 0.3g ascorbate tetraisopalmitate, 0.2g milk thistle seed oil, 0.1g passion fruit seed oil, 0.1g myrothamnus flabellifolia leaf / stem extract, and 0.01g sea fennel extract.

[0089] Example 6 A method for preparing a plant seed oil composition: The difference from Example 4 is that component B further includes 0.6g meadowfoam seed oil, 0.5g ascorbate tetraisopalmitate, 0.4g milk thistle seed oil, 0.15g passion fruit seed oil, 0.18g myrothamnus flabellifolia leaf / stem extract, and 0.02g sea fennel extract.

[0090] Example 7 A method for preparing a plant seed oil composition: The difference from Example 1 is that the Candida albicans fermented oil includes, by mass percentage, 40% Candida albicans / glucose / rapeseed oleate fermentation product, 16% olive fruit oil, 11% sunflower seed oil, 6% Angelica sinensis root extract, 4% Lithospermum erythrorhizon root extract, 2.5% Glycyrrhiza uralensis root extract, and 20.5% Aralia elata kernel oil.

[0091] Example 8 A method for preparing a plant seed oil composition: The difference from Example 7 is that the Candida albicans fermented oil includes, by mass percentage, 38% Candida albicans / glucose / rapeseed oleate fermentation product, 15% olive fruit oil, 10% sunflower seed oil, 5% Angelica sinensis root extract, 3.5% Lithospermum erythrorhizon root extract, 2% Glycyrrhiza uralensis root extract, and 26.5% Aralia elata kernel oil.

[0092] Example 9 A method for preparing a plant seed oil composition: The difference from Example 7 is that the Candida albicans fermented oil includes, by mass percentage, 42% Candida albicans / glucose / rapeseed oleate fermentation product, 18% olive fruit oil, 12% sunflower seed oil, 7% Angelica sinensis root extract, 4.5% Lithospermum erythrorhizon root extract, 3% Glycyrrhiza uralensis root extract, and 13.5% Aralia elata kernel oil.

[0093] Example 10 A method for preparing a plant seed oil composition: differs from Example 1 in that the Candida bombicola yeast-derived ferment oil is treated by the method of Preparation Example 2.

[0094] Example 11 A method for preparing a plant seed oil composition: differs from Example 10 in that the Candida bombicola yeast-derived ferment oil is treated by the method of Preparation Example 3.

[0095] Example 12 A method for preparing a plant seed oil composition: differs from Example 10 in that the Candida bombicola yeast-derived ferment oil is treated by the method of Preparation Example 4.

[0096] Example 13 A method for preparing a plant seed oil composition: differs from Example 10 in that the Candida bombicola yeast-derived ferment oil is treated by the method of Preparation Example 5.

[0097] Example 14 A method for preparing a plant seed oil composition: differs from Example 10 in that the Candida bombicola yeast-derived ferment oil is treated by the method of Preparation Example 6.

[0098] Example 15 A method for preparing a plant seed oil composition: differs from Example 10 in that the Candida bombicola yeast-derived ferment oil is treated by the method of Preparation Example 7.

[0099] Example 16 A method for preparing a plant seed oil composition: differs from Example 1 in that the hydroxyl retinylidene pinacolate is encapsulated in phospholipid-chitosan complex vesicles, prepared by the method of Preparation Example 8.

[0100] Example 17 A method for preparing a plant seed oil composition: differs from Example 16 in that the hydroxyl retinylidene pinacolate is encapsulated in phospholipid-chitosan complex vesicles, prepared by the method of Preparation Example 9.

[0101] Example 18 A method for preparing a plant seed oil composition: differs from Example 16 in that the hydroxyl retinylidene pinacolate is encapsulated in phospholipid-chitosan complex vesicles, prepared by the method of Preparation Example 10.

[0102] Comparative Example 1 A method for preparing a plant seed oil composition: differs from Example 1 in that component A is not added.

[0103] Comparative Example 2 A method for preparing a plant seed oil composition: different from example 1 is that no Candida bombicola ferment oil is added.

[0104] Comparative example 3 A method for preparing a plant seed oil composition: different from example 1 is that no isosorbide dimethyl ester is added.

[0105] Performance detection test The items requiring human testing in the detection method of the present application each use 12 skin-healthy volunteers (25-45 years old) in each group, and each group of volunteers tests the product of one example (or comparative example). The test results are truncated mean values, as shown in the following table: Table 1 Detection method Test item Method Conditions / instruments Transdermal absorption rate Franz diffusion cell method (ex vivo human abdominal skin, thickness 0.8 mm) Receiving solution: PBS: ethanol = 7:3, 32°C, 24 h Barrier function Stratum corneum water content (Corneometer® CM825) Transepidermal water loss (TEWL, Tewameter® TM300) 2 times a day, 0.5 g each time, for 7 consecutive days Anti-wrinkle efficacy Fibroblast collagen synthesis (ELISA, human dermal fibroblasts) VISIA-CR crows feet area analysis Cell experiment: 0.1% sample treatment for 72 h Human: 28-day trial, 1 g per day Antioxidant capacity DPPH radical scavenging rate HaCaT cell ultraviolet-induced ROS inhibition (flow cytometry) Sample concentration 1 mg / mL The experimental data of the present application are shown in the following table: Transdermal absorption rate (%) Barrier function (stratum corneum water content, A.U.) Barrier function (TEWL, g / h·m²) Anti-wrinkle efficacy (collagen synthesis, ng / mL) Anti-wrinkle efficacy (crows feet reduction, %) Antioxidant capacity (DPPH scavenging rate, %) Antioxidant capacity (ROS inhibition rate, %) Example 1 42.5 58.2 12.8 185.5 15.2 65.3 48.7 Example 2 38.1 55.0 13.5 175.8 13.8 62.1 45.2 Example 3 43.8 56.8 13.0 188.1 15.6 66.5 50.1 Example 4 43.0 65.5 10.5 187.0 15.5 82.4 65.3 Example 5 41.8 61.2 11.8 183.2 14.8 75.6 58.9 Example 6 43.8 66.8 10.2 189.1 16.1 85.1 68.5 Example 7 43.2 59.5 12.5 187.5 15.8 68.4 52.1 Example 8 41.5 57.8 13.0 182.0 14.5 66.0 49.5 Example 9 44.0 58.9 12.7 188.0 15.9 69.5 53.8 Example 10 48.5 59.0 12.6 188.0 16.0 70.2 60.5 Example 11 46.2 58.5 12.7 186.5 15.7 68.8 55.8 Example 12 47.8 58.8 12.7 187.2 15.9 69.5 58.2 Example 13 49.1 59.1 12.6 188.2 16.1 70.5 61.0 Example 14 46.5 58.7 12.8 186.8 15.6 68.0 56.5 Example 15 50.2 60.1 12.3 190.5 16.5 75.8 70.2 Example 16 52.8 60.5 12.0 225.0 19.8 66.5 50.2 Example 17 50.5 59.8 12.2 215.5 18.5 65.8 49.5 Example 18 51.2 60.2 12.1 220.8 19.2 66.0 49.8 Comparative Example 1 25.3 45.2 16.5 150.2 10.5 60.1 42.5 Comparative Example 2 41.0 52.5 14.2 170.5 13.0 58.5 40.1 Comparative Example 3 30.5 56.5 13.8 180.2 14.5 64.2 47.5 As can be seen from the combination of example 1 and comparative example 1 and table 2, the lack of component A (isononyl isononanoate and frake bark extract) results in a significant decrease in the transdermal absorption rate, barrier repair function, and anti-wrinkle efficacy of the composition. The transdermal absorption rate decreases significantly from 42.5% to 25.3%, indicating that isononyl isononanoate as a light carrier and penetration enhancer is essential for the transdermal delivery of active ingredients; at the same time, the decrease in the water content of the stratum corneum and the increase in the TEWL value prove that the softening of the stratum corneum and the auxiliary barrier repair function are indispensable; in addition, the collagen synthesis amount and the improvement effect on crow's feet are also significantly weakened, further confirming the key role of component A in synergistically improving the overall efficacy.

[0106] As can be seen from the combination of example 1 and comparative example 2 and table 2, the lack of Candida bombicola ferment oil significantly affects the antioxidant and anti-wrinkle performance of the composition. The DPPH clearance rate and cell ROS inhibition rate decrease from 65.3%, 48.7% to 58.5%, 40.1%, respectively, indicating that the active ingredients rich in the ferment oil have significant antioxidant potential; at the same time, the collagen synthesis amount decreases from 185.5 ng / mL to 170.5 ng / mL, and the percentage of crow's feet reduction decreases from 15.2% to 13.0%, indicating that the nutritional ingredients and microecological regulating ingredients in the ferment oil have a promoting effect on skin cell viability and collagen synthesis.

[0107] It can be seen from the combination of Example 1 and Comparative Example 3 and Table 2 that the transdermal absorption rate of the composition is significantly reduced from 42.5% to 30.5% after omitting isosorbide dimethyl ether, which proves that it plays a decisive role in expanding the lipid channel of the stratum corneum and improving the penetration efficiency of oil-soluble ingredients as a penetration enhancer. Although the anti-wrinkle related indicators have a relatively small decrease, the decrease in penetration rate directly affects the effective concentration of active ingredients, thereby restricting the full play of the overall efficacy.

[0108] It can be seen from the combination of Examples 1-3 and Table 2 that when the content of each component is within the range of Example 1, the comprehensive performance is best, and the component ratio range determined by the present application has reached an optimized state in balancing efficacy and safety.

[0109] It can be seen from the combination of Examples 1, 4, 5, 6 and Table 2 that the addition of white pool seed oil, ascorbic acid tetraisopalmitate and other active ingredients in component B can significantly enhance the antioxidant and barrier repair ability of the composition. The DPPH clearance rate and ROS inhibition rate of Examples 4 and 6 are much higher than that of Example 1, and the stratum corneum water content is higher and the TEWL value is lower, which proves that these components can strengthen the barrier function and antioxidant defense network of the skin by supplementing lipids, scavenging free radicals and inhibiting inflammatory pathways.

[0110] It can be seen from the combination of Examples 1, 7, 8, 9 and Table 2 that the specific composition of the honey-derived Candida ferment oil has a subtle influence on the comprehensive performance of the product. Example 7 shows balanced anti-wrinkle and antioxidant performance; Example 8 has a slight decrease in various indicators; and Example 9 shows better transdermal permeability and antioxidant properties. This indicates that there is an optimal range for the ratio of fermentation products to plant oils and extracts in the ferment oil, and the ratio of Example 7 is more effective in synergistic penetration, anti-inflammatory and nutritional supplementation.

[0111] It can be seen from the combination of Examples 1, 10-14 and Table 2 that low-temperature plasma treatment of the honey-derived Candida ferment oil can further improve its efficacy. Compared with Example 1 without treatment, the transdermal absorption rate of all treatment groups is improved, and the antioxidant capacity is also significantly enhanced. Among them, Example 13 has the best effect, which shows that a thinner oil film thickness is beneficial to the uniformity and efficiency of plasma treatment; but from the comprehensive perspective of production cost and treatment effect, the corresponding treatment method adopted in Example 10 is better than Examples 11-14.

[0112] It can be seen from Examples 10, 15 and Table 2 that the bioactivity of the fermented oil can be further stimulated by using a helium-oxygen mixed gas in the plasma treatment compared with pure helium gas. The transdermal absorption rate, antioxidant and anti-wrinkle indexes of Example 15 are all superior to those of Example 10, proving that the lipid peroxide generated at a specific oxygen concentration can act as a signal molecule to activate the antioxidant pathway of the skin itself, thereby achieving a "bioactivation" effect.

[0113] It can be seen from Examples 1, 16-18 and Table 2 that coating hydroxyl pinacolyl retinoate (HPR) in phospholipid-chitosan complex vesicles can greatly improve its transdermal efficiency and anti-wrinkle efficacy. The transdermal rate of Example 16 is as high as 52.8%, the amount of collagen synthesis is increased to 225.0 ng / mL, and the percentage of fish tail wrinkle reduction is 19.8%, which is significantly better than that of Example 1 without coating. This shows that the nanovesicle technology not only protects the activity of HPR, but also promotes its penetration of the stratum corneum and targeted release through electrostatic adsorption and nanosize effect. Examples 17 and 18 are slightly inferior, indicating that the mass ratio of phospholipid to cholesterol 8:2 is the best encapsulation ratio.

[0114] The specific embodiments are merely illustrative of the present application, and are not intended to limit the present application. Those skilled in the art can make modifications to the embodiments without creative contribution after reading the present specification, and the modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A plant seed oil composition, characterized in that, The component A comprises 32-35 parts of isononyl isononanoate and 0.01-0.05 parts of fraxinus excelsior bark extract by mass parts, the component C comprises 1.8-2.2 parts of hydroxypinacolone retinoate, 1-1.5 parts of candida bombicola ferment oil, 0.8-1.2 parts of sweet almond seed oil, 0.4-0.8 parts of spilanthes extract, 1.5-2.5 parts of isosorbide dimethyl ether, the component B comprises 55-65 parts of oil phase solvent; the hydroxypinacolone retinoate is coated in phospholipid-chitosan complex vesicles, and the specific method for coating the hydroxypinacolone retinoate in the phospholipid-chitosan complex vesicles is: Hydrogenated soybean phospholipid, cholesterol and hydroxypinacolone retinoate are dissolved in anhydrous ethanol at a mass ratio of (7.5-8.5):2:1 to form an oil phase liquid in a water bath at 50-55 ℃; Chitosan with a deacetylation degree of ≥95% is dissolved in a 0.3% mass concentration lactic acid aqueous solution with a pH of 4.0-4.5, and the chitosan concentration is controlled at 1.5-2.0 wt% to obtain an aqueous phase liquid; The oil phase liquid and the water phase liquid are mixed at a shear rate of 10 4 s-1 to form prevesicles with a particle size of 80-120 nm. A 0.4%-0.6% mass concentration sodium tripolyphosphate aqueous solution is added dropwise to the pre-vesicle system, the mass ratio of chitosan to sodium tripolyphosphate is controlled at 5:1, and the stirring reaction is carried out at 25-28 ℃ for 30-50 min at a pH of 5.0-5.

5.

2. The plant seed oil composition of claim 1, wherein: The component B further comprises 0.4-0.6 parts of white pool flower seed oil, 0.3-0.5 parts of ascorbyl tetraisopalmitate, 0.2-0.4 parts of silybum marianum seed oil, 0.1-0.15 parts of physalis pruinosa seed oil, 0.1-0.18 parts of myrobalan leaf / stem extract, and 0.01-0.02 parts of sea fennel extract.

3. The plant seed oil composition of claim 1, wherein: The candida bombicola ferment oil comprises, by mass percentage, 38%-42% of candida bombicola / glucose / canola oil methyl ester fermentation product, 15%-18% of olea europaea fruit oil, 10%-12% of helianthus annuus seed oil, 5%-7% of angelica gigas nakai root extract, 3.5%-4.5% of radix lithospermi extract, 2%-3% of radix glycyrrhizae extract, and the balance of acacia rigens kernel oil.

4. The plant seed oil composition of claim 1, wherein: The candida bombicola ferment oil needs to be further treated as follows: The candida bombicola ferment oil is preheated at 40-45 ℃ and filtered; The filtered ferment oil is exposed to a low-temperature plasma field generated by dielectric barrier discharge in the form of a film with a thickness of ≤2 mm for treatment; The treatment power is 40-60 W, the treatment time is 5-10 min, and the treatment atmosphere is an inert gas; The treated ferment oil is cooled to room temperature under helium gas protection.

5. The plant seed oil composition of claim 4, wherein: The helium gas is doped with oxygen, and the oxygen volume ratio is 20%-30%.

6. A method of preparing a plant seed oil composition as claimed in any one of claims 1 to 5, characterised in that: The following steps are included: The component A is added to a container, heated to 85-90 ℃ to dissolve and disperse, cooled to 45-50 ℃, and then the component B and the component C are added in sequence and dispersed to be free of particles, and then cooled to 38 ℃ to obtain a plant seed oil composition.

7. Use of a plant seed oil composition as claimed in any one of claims 1 to 5, characterised in that: The plant seed oil composition is applied to an essence oil.