Kluyveromyces marxianus beta-glucan-enriched cubilose compound as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a cubilose compound rich in Kluyveromyces marxianus beta-glucan as well as a preparation method and application of the cubilose compound. According to the compound, kluyveromyces marxianus is used for fermenting or not fermenting a cubilose matrix, so that in-situ compounding and synergistic interaction of yeast-derived beta-glucan and cubilose-derived active ingredients (such as sialic acid and small molecule peptide) are realized. The preparation method comprises the following steps: cubilose pretreatment, yeast activation and inoculation, and post-treatment of a mixed product. Experiments prove that the compound has remarkable immune enhancement and skin health improvement effects, and can be used for preparing food, health care products, medicines or personal care products. The problems that a traditional cubilose product is low in absorption and utilization rate, single in function, uneven in physical mixing and the like are solved, and a novel efficient and high-valued cubilose deep processing technology is provided.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology. Specifically, this invention relates to a Kluyveromyces martensii-bird's nest complex rich in β-glucan, its preparation method, and its application in enhancing immunity and improving skin health. Background Technology

[0002] Max Kluyveromycin ( Kluyveromyces marxianus It is a generally recognized as safe (GRAS) yeast strain, characterized by rapid growth, a wide range of fermentation substrates, and excellent flavor.

[0003] Bird's nest, a traditional and precious tonic, mainly contains sialic acid, epidermal growth factor (EGF), and various amino acids. It has a long history of use in enhancing immunity, beautifying the skin, and delaying aging. Related studies have also confirmed its effects in promoting cell proliferation and regulating gut microbiota. However, natural bird's nest and traditional consumption methods still face several limitations: Firstly, traditional consumption mainly involves stewing, a complex process where the active ingredients are heat-sensitive. Prolonged high-temperature processing can reduce immune activity, and the large protein molecules are not easily absorbed directly by the body, requiring digestion and decomposition, resulting in low absorption and utilization rates. Secondly, its product form is relatively simple; while its nutritional value is high, its functional targeting is not clearly defined, mainly focusing on basic nourishment and having a weaker effect on systemic immune regulation. Furthermore, its scarcity and high price further limit its widespread application.

[0004] β-glucan, especially β-(1,3) / (1,6)-glucan derived from yeast, is an internationally recognized potent immunomodulator that can activate macrophages, natural killer cells (NK cells), and other immune systems, thereby enhancing the body's immunity. Furthermore, studies have found that β-glucan also possesses excellent skin moisturizing, barrier repair, and wound healing properties.

[0005] Currently, there are products on the market that simply physically mix bird's nest with β-glucan. However, this method has problems such as uneven dispersion of ingredients, poor stability, and possible unpleasant taste. More importantly, simple mixing is unlikely to produce synergistic effects.

[0006] Kluyveromyces marxianus itself is a good source of β-glucan. The present application innovatively uses the yeast to process bird's nest, aiming to develop a new type of composite product with synergistic effect, rich nutrition and easy absorption. β-glucan, especially β-glucan derived from Kluyveromyces marxianus, is an excellent natural immunomodulator. It can effectively activate immune cells such as macrophages and natural killer cells (NK cells), enhance the body's non-specific immune and specific immune response, and also has the effects of lowering blood sugar and lowering blood lipids. Compounding β-glucan with bird's nest can theoretically achieve synergistic effect of immune enhancement. SUMMARY

[0007] The present application aims to overcome the deficiencies of the prior art, and provides a Kluyveromyces marxianus-bird's nest composite rich in β-glucan, a preparation method and application thereof. Kluyveromyces marxianus is used to process bird's nest, and β-glucan is generated in situ and compounded with active ingredients of bird's nest. The obtained Kluyveromyces marxianus-bird's nest composite has a significant synergistic effect on immunity and beauty.

[0008] To achieve the above-mentioned purpose, the present application adopts the following technical solutions: On the one hand, the present application provides a composition comprising a Kluyveromyces marxianus-bird's nest composite, wherein the composition enhances the body's immunity and / or improves the skin health. Kluyveromyces marxianus

[0009] In some embodiments, the Kluyveromyces marxianus is high-β-glucan Kluyveromyces marxianus.

[0010] In some embodiments, the Kluyveromyces marxianus has a preservation number of CCTCC No. M 20241673.

[0011] In some embodiments, the active ingredients of the composition include yeast-derived β-glucan, and bird's nest-derived sialic acid and small molecule peptides.

[0012] In some embodiments, the composition further comprises a physiologically acceptable adjuvant and / or excipient.

[0013] In some embodiments, the physiologically acceptable adjuvant and / or excipient comprises one or more of other edible raw materials, seasonings, solvents, diluents, disintegrants, precipitation inhibitors, surfactants, glidants, binders, lubricants, dispersants, suspending agents, isotonic agents, thickening agents, emulsifiers, preservatives, stabilizers, hydrating agents, emulsification accelerators, buffers, coloring agents, flavoring agents, sweetening agents, ion exchangers, flavoring agents or antioxidants.

[0014] ​In some embodiments, the other edible raw materials include one or more of coconut milk, oat, spelt, glutinous rice, tremella, chain, and lily.

[0015] In another aspect, the present application provides a method for preparing the above-mentioned composition, comprising the following steps: S1. Pre-treatment of bird's nest substrate; S2. Inoculation of K. marxianus for fermentation or compounding; S3. Post-treatment.

[0016] In some embodiments, the S1 step is to physically break and / or enzymatically treat the bird's nest substrate to prepare a bird's nest slurry.

[0017] In some embodiments, the S2 step is to inoculate the K. marxianus strain into the bird's nest slurry for fermentation or compounding.

[0018] In some embodiments, the S3 step is to dry the mixture, or to centrifugally separate the mixture, to break the cell wall of the obtained yeast cells, to mix the broken cell wall with the supernatant, and to dry the mixture to obtain the composition.

[0019] In some embodiments, the method for preparing the composition comprises the following steps: S1. Pre-treatment of bird's nest substrate: physically break and / or enzymatically treat the bird's nest substrate to prepare a bird's nest slurry; S2. Inoculation: inoculate the K. marxianus strain into the bird's nest slurry for fermentation or compounding; S3. Post-treatment: dry the mixture, or centrifugally separate the mixture, to break the cell wall of the obtained yeast cells, to mix the broken cell wall with the supernatant, and to dry the mixture to obtain the composition.

[0020] In some embodiments, in step S2, the inoculation amount for fermentation or compounding is 3% to 10% (v / v), for example, it can be 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%, preferably 5%.

[0021] In some embodiments, in step S2, the fermentation temperature is 25°C to 30°C, for example, it can be 25°C, 26°C, 27°C, 28°C, 29°C, or 30°C, preferably 28°C.

[0022] In some embodiments, in step S2, the fermentation time is 0-12 hours, for example, it can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 hours, preferably 12 hours.

[0023] In some embodiments, in step S2, the pH of the fermentation is 5-7, for example, it can be 5, 5.5, 6, 6.5 or 7, preferably 5.5-6.5.

[0024] In some preferred embodiments, the preparation method comprises the following steps: S1. Edible bird's nest substrate pretreatment: according to the shape of the cleaned edible bird's nest, optionally performing physical crushing and / or enzymatic hydrolysis treatment to prepare an edible bird's nest slurry; S2. Inoculation: inoculating the activated K. marxianus suspension into the edible bird's nest slurry obtained in step S1, and performing aerobic fermentation at 25-30°C and pH 5.5-6.5 for 0-12 hours; S3. Post-treatment: after the fermentation is completed, the fermentation broth is directly dried; or the fermentation broth is centrifuged, the obtained yeast cells are broken, the broken cells are mixed with the fermentation supernatant, and then dried, thereby obtaining the composition.

[0025] In some preferred embodiments, the preparation method comprises the following steps: S1. Edible bird's nest substrate pretreatment: according to the shape of the cleaned edible bird's nest, optionally performing physical crushing and / or enzymatic hydrolysis treatment to prepare an edible bird's nest slurry; S2. Inoculation: inoculating the activated K. marxianus suspension into the edible bird's nest slurry obtained in step S1, and performing aerobic fermentation at 25-30°C and pH 5.5-6.5 for 0-12 hours; S3. Post-treatment: after the fermentation is completed, the fermentation broth is directly dried; or the fermentation broth is centrifuged, the obtained yeast cells are broken, the broken cells are mixed with the fermentation supernatant, and then dried, thereby obtaining the composition.

[0026] In another aspect, the present application provides the use of the above-mentioned composition or the composition prepared according to the above-mentioned method in the preparation of food, medicine, health care products or personal care products.

[0027] In some embodiments, the personal care product is a cosmetic product.

[0028] In some embodiments, the food, medicine or health care product is used for enhancing the immunity of the body.

[0029] In some embodiments, the food, medicine or health care product can improve the phagocytic activity of macrophages or promote the secretion of immune molecules.

[0030] In some embodiments, the immune molecules are TNF-α or IL-6 In some embodiments, the food, medicine, health care product or personal care product is used for improving the health of the skin.

[0031] In some embodiments, the improvement of skin health comprises moisturization or anti-aging.

[0032] Advantages of the present application Synergistic effect: By adding Kluyveromyces marxianus, the "in situ complexation" of β-glucan and active ingredients of bird's nest is achieved. The yeast can use bird's nest ingredients as a nutrient source during growth, and organically combines the β-glucan synthesized by itself with active peptides, sialic acid and other organic substances produced by the decomposition of bird's nest, possibly forming a new functional complex structure, producing an "1+1>2" immune and cosmetic synergistic effect.

[0033] Nutritional synergism: On the one hand, Kluyveromyces marxianus has high immune activity of β-glucan, which can form nutritional complementation with the active ingredients of bird's nest, strengthening the immune strengthening properties of bird's nest products; on the other hand, it can also be preferred to use the fermentation process to decompose the large molecular weight proteins in bird's nest into small molecular weight peptides and amino acids that are more easily absorbed, improving bioavailability. At the same time, fermentation itself can produce new beneficial metabolites (such as vitamins, organic acids), further enriching the nutritional value of the product.

[0034] Technical novelty: The application of microbial fermentation technology or bioactive products of fermentation to the deep processing of bird's nest has overturned the traditional physical mixing mode, and the process is green and efficient, providing a new path for the modernization of this traditional tonic.

[0035] Cost and taste optimization: Through the fermentation process, relatively less bird's nest raw material can be used in combination with the biosynthesis of yeast to achieve efficacy amplification, thereby reducing costs to some extent. At the same time, fermentation can improve the flavor of the product.

[0036] Preservation information The strain used in the present application is Kluyveromyces marxianus (CJA0020), with preservation number CCTCC No. M 20241673, which has been preserved in the China Center for Type Culture Collection (CCTCC) on July 22, 2024. Kluyveromyces marxianus DETAILED DESCRIPTION

[0037] For the purpose of clear and brief description, some features are described herein as part of the same or separate embodiments, however, it will be understood that the scope of the present application can include some embodiments having combinations of all or some of the described features. The technical solutions of the present application will be described clearly and completely below. Obviously, based on the specific embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0038] I. Definitions ​Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The following definitions are applied to the descriptions of the present application and throughout this document, and apply equally to both the singular and plural forms of the terms defined.

[0039] As used herein, the terms "comprising", "having", "including", and "containing" are to be construed as open-ended terms (i.e., meaning "including, but not limited to") unless otherwise noted. "And / or" is used to describe one or more associated conditions, for example, A and / or B means that A alone, B alone, or A and B together can be present. The term "or" is used in the alternative (i.e., the juxtaposition of A and B means that A alone, B alone, or A and B together can be present).

[0040] As used herein, the terms "optional", "any", "any of", or "any one of" mean that the event or circumstance that is described subsequently can occur, but need not necessarily occur. As used herein, "one", "an", and "the" are used interchangeably in this document to refer to one or more than one of the grammatical object of the article. "Plurality", "a plurality" or "multiple" refer to two or more than two objects, such as two, three, four, five, etc. The terms "one or more" and "at least one" can be used interchangeably.

[0041] As used herein, the description of the term "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that a particular feature, structure, material, or characteristic being described can be included in at least one embodiment or example of the present application. The illustrative appearances of the above-mentioned terms in this specification are not necessarily all referring to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples. Moreover, the usage of particular terms to describe particular features, structures, materials, or characteristics is not intended to limit the scope of the present application but rather to highlight different aspects of embodiments or examples of the present application.

[0042] As used herein, the term "about" means ± 20% of the numerical value of the number that it precedes. In some embodiments, the term "about" means ± 10% of the numerical value of the number that it precedes. In some embodiments, the term "about" means ± 5% of the numerical value of the number that it precedes.

[0043] As used herein, the term "composition" refers to a composition containing a Kluyveromyces martensii-bird's nest complex. In some embodiments, the composition further includes at least one physiologically acceptable excipient or excipient, including but not limited to: other edible ingredients, seasonings, solvents, diluents, disintegrants, precipitation inhibitors, surfactants, flow aids, binders, lubricants, dispersants, suspending agents, isotonic agents, thickeners, emulsifiers, preservatives, stabilizers, hydrating agents, emulsification accelerators, buffers, colorants, flavorings, sweeteners, ion exchangers, flavoring agents, or antioxidants. These ingredients work together to provide a more comprehensive effect.

[0044] II. Examples The present invention will be described in detail below through specific embodiments. It should be understood that the following embodiments are for explanation and illustration only and do not limit the scope of the present invention in any way.

[0045] Where specific methods, techniques, or conditions are not specified in the examples, they shall be performed in accordance with the methods, techniques, or conditions described in the literature in this field or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0046] Example 1: Preparation of a high β-glucan-rich Kluyveromyces martensii bird's nest complex Take 10 grams of dried bird's nest, soak and clean it, then add 200 mL of purified water and blend it into a paste using a homogenizer.

[0047] The slurry was enzymatically hydrolyzed with papain (0.5%) at 50°C for 2 hours, then boiled for 10 minutes to sterilize, and cooled to 30°C to serve as the fermentation medium.

[0048] Max Kluyveromyces, which contains high levels of β-glucan ( Kluyveromyces marxianus CJA0020 (deposited on July 22, 2024 at the China Center for Type Culture Collection (CCTCC, Wuhan University, Wuhan, China), accession number CCTCC No. M 20241673) was activated in YPD liquid medium at 30°C and 180 rpm for 16 hours.

[0049] The activated yeast liquid was inoculated into the bird's nest culture medium at a rate of 5%, and fermented with shaking at 28℃, 200 rpm, and pH 5.5-6.5 for 0-12 hours.

[0050] After fermentation, the fermentation broth was subjected to cell wall disruption treatment, followed by high-temperature and high-pressure sterilization and vacuum freeze-drying at -50℃ to obtain a light yellow powdery Kluyveromyces martensii-bird's nest complex (experimental group).

[0051] Three comparative examples were also used in this study: Comparative Example A: High beta-glucan K. marxianus cultured normally.

[0052] Comparative Example B: The non-fermented bird's nest enzyme solution was directly freeze-dried to obtain a bird's nest extract.

[0053] Comparative Example C: The bird's nest extract powder of Comparative Example A and the high beta-glucan K. marxianus powder of Comparative Example B were simply physically mixed in the estimated ratio in the fermentation product.

[0054] Example 2: Verification of the immune efficacy of high beta-glucan K. marxianus bird's nest compound A mouse macrophage RAW264.7 model was used.

[0055] The mice were grouped: blank control group, experimental group (product of Example 1, 100 μg / mL), Comparative Example A group (beta-glucan, 100 μg / mL), Comparative Example B group (bird's nest extract, 100 μg / mL), Comparative Example C group (physical mixture, 100 μg / mL).

[0056] Detection index: phagocytic activity was detected by neutral red phagocytosis experiment; the secretion levels of TNF-α and IL-6 in the cell supernatant were detected by ELISA method.

[0057] The results are shown in Table 1. The experimental group was significantly better than each comparative example group in promoting macrophage phagocytosis and secreting immune factors, indicating that the compound produced by fermentation has a synergistic immune enhancing effect. It was also found that the physical mixture of oats and K. marxianus in Comparative Example C also had a good immune boosting effect, showing a more processing-friendly market value. This experiment shows that the compounding of K. marxianus and bird's nest shows a 1+1>2 effect, and has a high market value.

[0058] Table 1: Effect of each group on immune indicators of macrophages

[0059] Example 3: Preparation of a coconut milk oat bird's nest containing K. marxianus and flavor evaluation The product formula is as follows: 1. Raw materials Base material and ratio: White granulated sugar 7.0%, Pure water 79.9% Coconut milk 13% Gellan gum 0.03% Sodium bicarbonate 0.07% The above raw materials are mixed according to the proportion, and then passed through a homogenizer with a total pressure of 320-360 bar (second pressure: 50-60 bar). The homogenized base material is divided into about 200-250 g / bowl.

[0060] 2. Auxiliary materials Oat grains 8.3 g / bowl, cooked for 15 minutes, and drained for standby Wild rice 3.3 g / bowl, washed and drained for standby Glutinous rice 2.8 g / bowl, washed and drained for standby Silver fungus silk 0.5 g / bowl, soaked for 0.5 hours, and drained for standby Lotus seeds 3 / bowl Bulb 3 / bowl Yan broken 200 mg / bowl High beta-glucan Kluyveromyces marxianus 1.0-2.0 g / bowl After the above raw materials are pretreated and weighed, they are added to the base material bowl, and a composite aluminum foil film is used for sealing. A vacuum sealer or a hot pressure sealer is used for sealing operation to ensure that the seal is tight and there is no leakage. High temperature and high pressure curing (121℃, 20 min, pressure 0.24 Mpa) is performed to achieve the commercial sterile state of the product and prolong the shelf life.

[0061] To scientifically verify the flavor improvement effect of the Kluyveromyces marxianus fermentation process in the present application, the following control experiment is designed: 1. Sample preparation: Experimental group: Kluyveromyces marxianus is inoculated into the adjusted mixture, and stirred uniformly.

[0062] Control group: Except for the addition of Kluyveromyces marxianus, the rest of the process is exactly the same. That is, after the base material is prepared, no yeast is added, and the homogenization, filling, curing and sterilization are directly performed.

[0063] 2. Sensory evaluation method An evaluation team consisting of 10 trained sensory evaluators is formed. Using the quantitative descriptive analysis method (Quantitative Descriptive Analysis, QDA), the intensity scores of key flavor attributes such as milk aroma, fruit sweet aroma, body, and off-flavor (cooking flavor / raw flavor) of the samples are evaluated, with the score being 0-10 points. The higher the score, the stronger the flavor attribute.

[0064] The sensory evaluation results are shown in the following table. The experimental group scored significantly higher in the positive flavor dimension than the control group, and the cooking flavor and raw flavor in the off-flavor dimension were significantly lower than the experimental group, indicating that the addition of high beta-glucan Kluyveromyces marxianus had a positive effect on the flavor of bird's nest porridge.

[0065] Table 2: Sensory evaluation results

[0066] Example 4: Application of the bird's nest compound rich in Kluyveromyces marxianus β-glucan in improving skin health This example aims to verify the innovative efficacy of the Kluyveromyces marxianus-bird's nest compound prepared by the present application (hereinafter referred to as "the compound of the present application") in promoting skin moisturization and delaying aging through in vitro cell experiments and human statistical tests.

[0067] 4.1. In vitro cell experiment This experiment is used to evaluate the effect of the Kluyveromyces marxianus-bird's nest compound on the proliferation of artificial skin fibroblasts and the secretion of hyaluronic acid.

[0068] Experimental samples: Experimental group: Kluyveromyces marxianus-bird's nest compound prepared in Example 1.

[0069] Comparative Example A: high β-glucan Kluyveromyces marxianus (same as Example 2).

[0070] Comparative Example B: bird's nest extract (same as Example 2).

[0071] Comparative Example C: physical mixture of Comparative Example A and Comparative Example B (same as Example 2).

[0072] Method: The artificial skin fibroblast (HSF cell) model was used. The cells were divided into a blank control group, an experimental group (100 μg / mL), a Comparative Example A group (100 μg / mL), a Comparative Example B group (100 μg / mL), and a Comparative Example C group (100 μg / mL). After 48 hours of culture, the CCK-8 method was used to detect the cell proliferation rate, and the ELISA kit was used to detect the content of hyaluronic acid (HA) in the cell culture supernatant.

[0073] Results: As shown in Table 3 below, the experimental group can significantly promote the proliferation of fibroblasts and the secretion of hyaluronic acid, far exceeding each comparative example group and the blank control group. This indicates that the compound of the present application can fundamentally improve the skin moisturizing ability by promoting the activity of key skin cells and the synthesis of endogenous moisturizing factors, and its effect cannot be achieved by simple physical mixing, confirming the synergistic effect of in-situ fermentation and compounding.

[0074] Table 3: Effect of each group on fibroblast activity and hyaluronic acid secretion (mean ± SD, n=6)

[0075] 4.2. Skin moisture content and wrinkle improvement evaluation This experiment was used to evaluate the effect of the sample of Example 3, i.e., the Kluyveromyces marxianus-cordyceps complex, on the skin moisture content and wrinkle improvement of the users.

[0076] Methods: 40 healthy female volunteers aged 35-55 years old with dry and fine lines on the skin were recruited and randomly divided into two groups. The experimental group used one box of the complex of the application every day, and the control group used a cordyceps product without the high-beta-glucan Kluyveromyces marxianus of the application. The test period was 8 weeks. Before the start of the test, at 4 weeks and 8 weeks, the water content of the stratum corneum was detected using a skin moisture tester (Corneometer® CM 825 from Germany CK Company), and the number and depth of wrinkles in the corner of the eye were detected using a skin rapid imaging analysis system (VISIA®-CR from the United States Canfield Scientific Company).

[0077] Results: As shown in Table 4 below, after 4 weeks and 8 weeks of use, the water content of the stratum corneum of the volunteers in the experimental group was significantly improved, and the number and depth of wrinkles in the corner of the eye were statistically significantly reduced. The control group had no significant changes in each index. No adverse reactions occurred in all volunteers during the test period.

[0078] Table 4: Skin improvement effect of human trial test (mean ± SD, n = 20 / group)

[0079] Note: Compared with the baseline at 0 weeks, **p < 0.01, *p < 0.05. 4.3. Conclusion This example proves through rigorous in vitro and in vivo experiments that the cordyceps complex rich in Kluyveromyces marxianus beta-glucan provided by the application can synergistically promote the activity of skin fibroblasts and significantly increase the generation of endogenous skin moisturizing substance, i.e., hyaluronic acid. In human trials, the complex showed excellent skin moisturizing and wrinkle-reducing effects. This fully demonstrates that the application has clear innovative role and great application potential in improving skin health and delaying skin aging.

[0080] In summary, the present application realizes in-situ complexation and synergistic effect of β-glucan and active ingredients (such as sialic acid and small molecular peptides) of bird's nest by using Kluyveromyces marxianus to ferment or compound the pretreated bird's nest. The process not only improves the bioavailability of the nutritional ingredients of bird's nest, but also possibly generates new functional structures. The product shows significantly better immune enhancement (such as promoting macrophage phagocytosis, secreting TNF-α and IL-6) and skin health improvement effects than single ingredients or physical mixtures in cell experiments. The present application provides an innovative path for developing bird's nest deep processing products with clear efficacy and easy absorption.

[0081] The specific embodiments of the present application are described in detail above, but they are only examples, and the present application is not limited to the above described specific embodiments. Any equivalent modifications and substitutions made by those skilled in the art to the present application are also within the scope of the present application. Therefore, equivalent transformations and modifications made without departing from the spirit and scope of the present application are also covered within the scope of the present application.

Claims

1. A composition comprising a Kluyveromyces marxianus (K. marxianus) - bird's nest complex, characterized in that, Kluyveromyces marxianus The composition enhances immunity and / or improves skin health, the Kluyveromyces marxianus is high-beta-glucan Kluyveromyces marxianus, and the Kluyveromyces marxianus has a preservation number of CCTCC No. M20241673. ​ 2. The composition of claim 1, wherein, The active ingredients of the composition include yeast-derived beta-glucan, and edible bird's nest-derived sialic acid and small molecule peptide.

3. Composition according to claim 1 or 2, characterized in that The composition further includes one or more of physiologically acceptable adjuvants and / or excipients, including other edible raw materials, seasonings, solvents, diluents, disintegrants, precipitation inhibitors, surfactants, glidants, binders, lubricants, dispersants, suspending agents, isotonic agents, thickening agents, emulsifiers, preservatives, stabilizers, hydrating agents, emulsification accelerators, buffers, colorants, flavorings, sweeteners, ion exchangers, flavor correctors, or antioxidants, and the other edible raw materials include one or more of coconut milk, oatmeal, wheat germ, glutinous rice, tremella, chain, and lily.

4. Process for the preparation of a composition according to claim 1 or 2, characterised in that, The method includes the following steps: S1. Pre-treatment of edible bird's nest substrate; S2. Inoculation of Kluyveromyces marxianus for fermentation or compounding; S3. Post-treatment.

5. The production method according to claim 4, characterized by, The S1 step is to physically break and / or enzymatically treat the edible bird's nest substrate to prepare an edible bird's nest slurry; The S2 step is to inoculate the Kluyveromyces marxianus strain into the edible bird's nest slurry for fermentation or compounding; and / or The S3 step is to dry the mixture, or to centrifugally separate the mixture, to break the cell wall of the obtained yeast cells, to mix the broken cell wall with the supernatant, and to dry the mixture to obtain the composition.

6. The preparation method according to claim 5, characterized in that, The preparation method includes the following steps: S1. Pre-treatment of edible bird's nest substrate: physically break and / or enzymatically treat the edible bird's nest substrate to prepare an edible bird's nest slurry; S2. Inoculation: inoculate the Kluyveromyces marxianus strain into the edible bird's nest slurry for fermentation or compounding; S3. Post-treatment: dry the mixture, or centrifugally separate the mixture, to break the cell wall of the obtained yeast cells, to mix the broken cell wall with the supernatant, and to dry the mixture to obtain the composition.

7. The production method according to claim 5 or 6, characterized by, In step S2, the inoculation amount for fermentation or compounding is 3% to 10% (v / v), the fermentation temperature is 25°C to 30°C, the fermentation time is 0-12 hours, and the fermentation pH is 5-7.

8. Use of the composition of any one of claims 1-3 or the composition prepared by the method of any one of claims 4-7 in the preparation of a food, a drug, a health product, or a personal care product.

9. Use according to claim 8, characterized in that, The food, drug, or health product is used to enhance immunity.

10. Use according to claim 8, characterized in that, The food, drug, health product, or personal care product is used to improve skin health, and the improvement of skin health includes moisturizing or anti-aging.

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