Extraction device for benzothiazole compound in ryegrass hydroponic solution

Abstract

The invention relates to the technical field of experimental extraction devices, in particular to an extraction device for benzothiazole compounds in ryegrass water culture solution, which comprises a culture solution treatment bin and an SPE (Solid Phase Extraction) column, and further comprises a material conveying mechanism for conveying the SPE column, a plurality of liquid storage pipes arranged above the SPE column and an extrusion device. According to the device and method for extracting the benzothiazole compound in the ryegrass hydroponic solution, through a meshing transmission structure of a movable toothed plate and an outer gear, in the process that a sliding block transversely transfers an SPE column, the internal structure of a stirring part is synchronously driven to rotate, a formed three-dimensional stirring net can achieve radial disturbance and axial liquid flow circulation at the same time, and before the hydroponic solution is transferred, the internal structure of the stirring part is driven to rotate; particle aggregation is broken in real time, sediment is prevented from blocking the solid-phase filler, the mass transfer efficiency of benzothiazole is dynamically enhanced, and the recovery rate of benzothiazole is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of experimental extraction devices, in particular to an extraction device for benzothiazole compounds in ryegrass hydroponics solution. BACKGROUND

[0002] In the ryegrass hydroponics system, benzothiazole compounds are secreted by the root system as allelochemicals or metabolites, and their extraction and separation are the key prerequisite for biological activity research. Due to the complexity of the hydroponics solution, the target compounds often exist in trace amounts and are easily interfered by humic acid, inorganic salt, etc. Therefore, high-efficiency extraction methods are needed to achieve separation and enrichment. Solid phase extraction (SPE) has become a common technology for the extraction of such compounds due to its advantages of selective adsorption, low solvent consumption, and simple operation.

[0003] The patent with the application number CN202421901027.3 discloses a solid phase extraction filling device, which includes a shell, a material box fixedly connected to the shell, a plurality of discharge holes formed in the material box, a first partition plate and a second partition plate fixedly connected in the shell, and a feeding mechanism slidingly arranged between the first partition plate and the second partition plate. The feeding mechanism can quickly fill multiple empty column tubes to obtain a solid phase extraction column, and a vibration motor is used to make the adsorbent material more uniform and compact.

[0004] However, the existing solid phase extraction equipment has two major defects: first, the solvent transfer and flow rate control rely on manual or discrete driving systems, and the injection pump / vacuum device needs to be repeatedly switched during operation, which not only leads to complicated steps, but also causes cross contamination due to residual pipeline. The error of manual flow rate adjustment is as high as ±5% (such as more than 2mL / min in the activation stage, which will reduce the activation efficiency of the bonding site), and second, the sample pretreatment and extraction process are disconnected. The particle settling rate increases when the hydroponics solution is at rest, which can block the micropores of the solid phase filler of the SPE column. The external oscillation device requires independent energy consumption and increases the mass transfer delay, resulting in low recovery rate of benzothiazole, which is difficult to meet the detection requirements.

[0005] In view of this, we propose an extraction device and method for benzothiazole compounds in ryegrass hydroponics solution. SUMMARY

[0006] The purpose of the present application is to provide an extraction device for benzothiazole compounds in ryegrass hydroponics solution, which uses an electric push rod to drive a tooth plate to complete multiple lifting actions to solve the problems raised in the background technology.

[0007] To achieve the above-mentioned purpose, on the one hand, the present application provides the following technical solutions: The extraction device for benzothiazole compounds in ryegrass hydroponics solution comprises a hydroponics solution treatment bin and an SPE column, and further comprises a material conveying mechanism for conveying the SPE column, a plurality of liquid storage tubes arranged above the SPE column, and a squeezing device. The material conveying mechanism comprises an electric push rod and a toothed plate at the top end of the electric push rod. The liquid storage pipe comprises a fixed pipe body, a plug rod arranged below the fixed pipe body, a bent plate sleeved on the outer end of the plug rod, and a stirring part arranged in the middle part of the liquid storage pipe. The upper end of the bent plate is slidably connected with the side of the toothed plate, the electric push rod drives the toothed plate to move upwards, the protruding rod moves along the inclined groove, the plug rod moves horizontally, the through hole is communicated with the vertical pipe of the cross pipe body, the solution in the fixed pipe body falls into the SPE column, and the toothed plate drives the stirring part to stir the solution in the middle part of the liquid storage pipe when the toothed plate moves horizontally. The extrusion device comprises a pair of racks, an internal gear arranged between the pair of racks, and a piston plate moving with the rear rack. The electric push rod drives the toothed plate to move upwards, the front rack is engaged with the internal gear to drive the rear rack to move downwards, and the piston plate drives the solution in the SPE column to flow out at a fixed flow rate.

[0008] In the technical scheme of the present application, the culture solution treatment bin comprises a bin body, a fixed frame welded on the outer wall of the bin body, a partition plate arranged below the fixed frame and welded with the bin body, a plurality of limiting frames regularly connected with the outer wall of the bin body, a plurality of sealing plugs connected with the top surface of the bin body, and a bent plate welded on the front wall of the bin body.

[0009] In the technical scheme of the present application, the bin body is internally provided with a front-to-rear through sliding groove, and the bin body is further provided with a placement groove on the front side of the sliding groove.

[0010] The above-mentioned arrangement provides a platform for integrating solvents used in the extraction process of thiazole compounds, and cooperates with the material conveying mechanism to provide a basis for solid-phase extraction of thiazole compounds.

[0011] In the technical scheme of the present application, the material conveying mechanism further comprises a motor, a lead screw coaxially connected with the output shaft of the motor, and a sliding block sleeved on the outer side of the lead screw and slidably connected in the sliding groove and the placement groove.

[0012] This arrangement solves the three problems of positioning deviation, timing confusion and pollution risk caused by manual operation of traditional SPE equipment.

[0013] The SPE column comprises a column body inserted into the end of the slider horizontal plate, solid phase fillers placed in the column body for capturing benzothiazole compounds, and two filter screens clamped in the column body, which are located above and below the solid phase fillers.

[0014] This arrangement provides a basis for capturing benzothiazole compounds.

[0015] In the technical scheme of the present application, the liquid storage pipe further comprises a first spring adhered to the rear end of the insertion rod, the fixed pipe body is clamped to the inner bottom surface of the fixed frame, and the other end of the first spring is adhered to the pipe wall of the cross pipe body horizontal pipe.

[0016] In the technical scheme of the present application, the bending plate is slidingly connected to the inside of the limiting frame, the inclined groove is arranged in a manner that the front is higher and the rear is lower, and the size of the top end horizontal plate of the bending plate is smaller than the size of the plate surface groove.

[0017] The above arrangement prevents solvent residue and reduces cross-contamination rate between different solutions through transverse sealing reset of the insertion rod and cooperation of the extrusion device.

[0018] In the technical scheme of the present application, the stirring part comprises an outer gear, a rotating shaft clamped in the inner part of the outer gear, and two stirring blades rotating with the rotating shaft, further comprises a connecting rod connected between the two stirring blades, a round rod rotatingly connected to the outer side of the connecting rod, and a stirring ring rotatingly connected to the top end of the round rod, the stirring ring is slidingly connected to the inner part of the fixed pipe body, and the outer gear is engaged with the toothed plate.

[0019] This arrangement breaks particle agglomeration, enhances mass transfer, and improves the capture efficiency of benzothiazole.

[0020] In the technical scheme of the present application, the extrusion device further comprises a sliding rod clamped to the end of the rear side rack horizontal plate and a second spring sleeved on the outer side of the sliding rod, the two racks are centrally symmetrically distributed, the inner gear is engaged with the two racks, the size of the bottom end horizontal plate of the rack located at the front side is smaller than the size of the plate surface groove, the piston plate is clamped to the bottom end of the sliding rod, and the second spring provides an elastic force to push the rear side rack to move upward.

[0021] This arrangement can adjust the size of a pair of racks and an inner gear according to different steps, so as to ensure that different solvents can flow through the SPE column at corresponding flow rates.

[0022] On the other hand, the present application also provides a method for extracting benzothiazole compounds in ryegrass hydroponics solution, which uses the above-mentioned extraction device for benzothiazole compounds in ryegrass hydroponics solution and comprises the following steps: S1, first, the operator filters the ryegrass hydroponics liquid through a filter membrane, adjusts the pH value of the ryegrass hydroponics liquid, and adds an internal standard substance, i.e. deuterated benzothiazole, into the adjusted ryegrass hydroponics liquid to correct the recovery rate; S2, then, the operator first fills methanol, ultrapure water, treated ryegrass hydroponics liquid, weak solvent and strong solvent into the inside of a plurality of liquid storage tubes from left to right, and places the SPE column into the circular hole at the end of the sliding block; S3, then, the motor is started to drive the screw to rotate, so that the sliding block moves horizontally, and the electric push rod moves horizontally together with the tooth plate, and the SPE column is sent to the lower side of the liquid storage tube filled with methanol; S4, the electric push rod is controlled to move upwards, and the top horizontal plate of the bending plate located in the groove on the plate surface moves together, and the convex rod at the end of the insertion rod moves along the inclined groove, and the insertion rod moves horizontally in the cross pipe body, so that the through hole is communicated with the vertical pipe of the cross pipe body, and then the methanol is dropped into the inside of the SPE column, S5, then, the electric push rod is controlled to reset the tooth plate and the bending plate, and the motor is started to send the SPE column to the lower side of the extrusion device at the leftmost side; S6, the electric push rod is controlled to move upwards again, the front side of the tooth plate moves upwards, the rear side of the tooth plate moves downwards through the meshing internal gear drive, the sliding rod and the piston plate at the bottom end of the sliding rod move downwards, the methanol drops from the SPE column at a fixed flow rate, so that the solid phase filler is wetted, the bonding site is opened, and the activation of the solid phase filler is realized; S7, then, the above operation is repeated, the ultrapure water flows through the solid phase filler, replaces the activated solvent, and makes the internal environment of the solid phase filler consistent with the treated ryegrass hydroponics liquid; S8, during the process of controlling the motor to drive the tooth plate to move horizontally again, the tooth plate meshes with the external gear, drives the rotating shaft to rotate, and the stirring blade and the connecting rod therebetween rotate in the ryegrass hydroponics liquid, and the stirring ring is driven to disturb the solution up and down through the circular rod, so that the particle agglomeration is broken, the mass transfer is enhanced, and the benzothiazole capture efficiency is improved; S9, then, the tooth plate moves up and down again, the ryegrass hydroponics liquid flows through the solid phase filler, and the benzothiazole compound in the inside of the solid phase filler is selectively adsorbed on the solid phase filler, and the sample loading operation is completed; S10, then, the hydrophilic interferent is eluted from the solid phase filler by the weak solvent, and the benzothiazole compound is desorbed from the solid phase filler by the strong solvent; S11, finally, the operator takes the glass container at the rightmost side, sends it into the nitrogen blowing instrument, enriches the benzothiazole compound, and stores and saves it.

[0023] Compared with the prior art, the beneficial effects of the present application are: 1. The extraction device and method of benzothiazole compounds in ryegrass hydroponics solution, by driving the tooth plate with an electric push rod to complete a single lifting action, the solution in different storage tubes can drop at the same time, and a pair of toothed bars and internal gears of the extrusion device can form a speed change mechanism to convert the vertical movement of the tooth plate into the uniform downward pressure of the piston plate, and in actual use, by adjusting the size of a pair of toothed bars and internal gears in the extrusion device, it can ensure that different solvents can flow through the SPE column at a corresponding flow rate, and eliminate the error of manual operation, and realize the precise control of the flow rate of methanol activation, water balance, sample loading and other steps.

[0024] 2. The extraction device and method of benzothiazole compounds in ryegrass hydroponics solution, by moving the meshing transmission structure of the tooth plate and the external gear, the internal structure of the stirring part is driven to rotate synchronously during the transverse transfer of the slider and the SPE column, and the three-dimensional stirring net formed thereby can realize radial disturbance and axial liquid flow circulation at the same time, and before the transfer of the hydroponics solution, the particle agglomeration is broken in real time to prevent the solid phase filler from being blocked by precipitation, dynamically enhance the mass transfer efficiency of benzothiazole, and improve the recovery rate. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present application; Figure 2 It is a schematic diagram of the overall structure of the present application; Figure 3 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 4 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 3 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 5 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 6 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 7 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 8 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 9 Figure 8 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 10 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 11 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; Figure 12 It is a schematic diagram of the structure of the culture solution treatment bin in the present application; BRIEF DESCRIPTION OF DRAWINGS ​100, culture solution treatment bin; 110, bin body; 111, chute; 112, placing groove; 120, fixed frame; 130, partition; 131, funnel; 140, limiting frame; 150, sealing plug; 160, hanging frame; 200, material conveying mechanism; 210, motor; 220, screw rod; 230, sliding block; 240, electric push rod; 250, toothed plate; 251, plate surface groove; 300, SPE column; 310, column body; 320, filter screen; 400, liquid storage pipe; 410, fixed pipe body; 411, cross pipe body; 420, insertion rod; 421, through hole; 422, convex rod; 430, first spring; 440, bent plate; 441, inclined groove; 450, stirring part; 451, outer gear; 452, rotating shaft; 453, stirring blade; 454, connecting rod; 455, circular rod; 456, stirring ring; 500, extrusion device; 510, rack; 520, inner gear; 530, sliding rod; 540, piston plate; 550, second spring; 600, glass container. DETAILED DESCRIPTION

[0026] The technical solutions in the present application will be described clearly and completely below in conjunction with the drawings in the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work belong to the protection scope of the present application.

[0027] Please refer to Figures 1-4 The present embodiment provides technical solutions as shown in the drawings: The benzothiazole compound extraction device and method for ryegrass hydroponics solution comprises a culture solution treatment bin 100 and an SPE column 300, and further comprises a material conveying mechanism 200 for conveying the SPE column 300, a plurality of liquid storage pipes 400 arranged above the SPE column 300, and an extrusion device 500. Specifically, the culture solution treatment bin 100 comprises a bin body 110, a fixed frame 120 welded to the outer wall of the bin body 110, a partition 130 arranged below the fixed frame 120 and welded to the bin body 110, a plurality of limiting frames 140 regularly clamped to the outer wall of the bin body 110, a plurality of sealing plugs 150 clamped to the top surface of the bin body 110, and a hanging frame 160 welded to the front wall of the bin body 110. A plurality of glass containers 600 are regularly placed on the inner bottom surface of the bin body 110.

[0028] Further, the inside of the bin body 110 is provided with a front-to-rear through sliding groove 111, and the bin body 110 is further provided with a placement groove 112 at the front side of the sliding groove 111. The baffle 130 is provided with a funnel 131 above each of the glass containers 600. The positions of the plurality of limiting frames 140 correspond to the positions of the plurality of extrusion devices 500. The plurality of sealing plugs 150 are located above the liquid storage pipes 400.

[0029] Further, the sliding groove 111 and the placement groove 112 on the bin body 110 provide a sliding interval for the structure in the material conveying mechanism 200. The fixed frame 120 provides a placement platform for the plurality of liquid storage pipes 400 and the extrusion devices 500. The funnel 131 on the baffle 130 ensures that the discharged liquid can accurately fall into the glass container 600 below. After the sealing plug 150 is opened, the operator can conveniently add solvent to the liquid storage pipe 400. This setting integrates a platform for the solvent used in the extraction process of the thiazole compound, and cooperates with the material conveying mechanism 200 to provide a basis for the solid-phase extraction of the thiazole compound.

[0030] Referring to Figures 5-6 In the embodiment, the material conveying mechanism 200 includes an electric push rod 240 and a tooth plate 250 at the top end of the electric push rod 240.

[0031] Specifically, the material conveying mechanism 200 further includes a motor 210, a lead screw 220 coaxially connected to the output shaft of the motor 210, and a sliding block 230 sleeved outside the lead screw 220 and slidingly connected in the sliding groove 111 and the placement groove 112. The electric push rod 240 is fixedly connected to the top surface of the sliding block 230 by a screw. The outer wall of the tooth plate 250 is provided with a plate recess 251. The upper end of the bent plate 440 is slidingly connected with the plate recess 251.

[0032] Further, after the motor 210 is started, the lead screw 220 is driven to rotate, so that the sliding block 230 moves horizontally, and the electric push rod 240 moves horizontally together with the tooth plate 250, so that the SPE column 300 is sent to below the liquid storage pipe 400 loaded with different solutions. This setting solves the three problems of positioning deviation, time sequence confusion and pollution risk caused by manual operation of the traditional SPE equipment.

[0033] Referring to Figure 7 In the embodiment, the SPE column 300 includes a column body 310 inserted into the transverse plate end of the sliding block 230, a solid-phase filler placed in the column body 310 for capturing benzothiazole compounds, and two filter screens 320 clamped in the column body 310. The two filter screens 320 are located in the upper and lower directions of the solid-phase filler.

[0034] Further, the solid phase filler is activated, balanced, loaded, and eluted, and can capture the benzothiazole compound. The upper filter screen 320 is used to block suspended particles such as plant fibers, soil debris, and the like in the hydroponic solution, so as to prevent the internal micropores of the solid phase filler from being blocked. The lower filter screen 320 is used to prevent fine particles such as C18 silica gel or HLB polymer from leaking out of the column 310 with the solution, and this setting provides a basis for capturing the benzothiazole compound.

[0035] Referring to Figures 8-10 In the embodiment shown, the liquid storage tube 400 includes a fixed tube body 410, a plug rod 420 arranged below the fixed tube body 410, a bent plate 440 sleeved on the outer end of the plug rod 420, and a stirring part 450 arranged in the middle of the liquid storage tube 400. A cross tube body 411 is connected to the bottom end of the fixed tube body 410, the plug rod 420 slides in the horizontal tube of the cross tube body 411, a through hole 421 is formed in the plug rod 420, and a protruding rod 422 is integrally formed at the end of the through hole 421. The bottom of the bent plate 440 is provided with an inclined groove 441 for sliding the protruding rod 422.

[0036] Specifically, the liquid storage tube 400 further includes a first spring 430 adhered to the rear end of the plug rod 420, the fixed tube body 410 is connected to the inner bottom surface of the fixed frame 120, and the other end of the first spring 430 is adhered to the wall of the horizontal tube of the cross tube body 411.

[0037] Further, the bent plate 440 is slidably connected to the inside of the limiting frame 140, the inclined groove 441 is arranged in a manner that the front is higher and the rear is lower, and the top end of the bent plate 440 is smaller in size than the size of the plate surface groove 251.

[0038] Further, the upper end of the bent plate 440 is slidably connected to the side of the toothed plate 250, the electric push rod 240 controls the toothed plate 250 to drive the bent plate 440 to move upward, the protruding rod 422 moves along the inclined groove 441, the plug rod 420 is driven to move horizontally, the through hole 421 is communicated with the vertical tube of the cross tube body 411, the solution in the fixed tube body 410 is dropped into the SPE column 300, and the first spring 430 provides an outward elastic force for the plug rod 420 to ensure the sealing property of the plug rod 420 to the vertical tube of the cross tube body 411 in a natural state. This setting resets the horizontal sealing of the plug rod 420, cooperates with the extrusion device 500, and eliminates the solvent residue to reduce the cross contamination rate between different solutions.

[0039] Referring to Figures 8-11As shown, in the embodiment, the stirring part 450 comprises an outer gear 451, a rotating shaft 452 clamped inside the outer gear 451, and two stirring blades 453 rotating with the rotating shaft 452, further comprises a connecting rod 454 connected between the two stirring blades 453, a round rod 455 rotatingly connected to the outer side of the connecting rod 454, and a stirring ring 456 rotatingly connected to the top end of the round rod 455, the stirring ring 456 is slidingly connected to the inside of the fixed tube body 410, and the outer gear 451 is engaged with the toothed plate 250.

[0040] Further, when the toothed plate 250 moves horizontally, the stirring part 450 can also stir the solution in the middle liquid storage pipe 400, specifically, the toothed plate 250 will engage the outer gear 451 to drive the rotating shaft 452 to rotate, so that the stirring blades 453 and the connecting rod 454 therebetween rotate inside the ryegrass hydroponics solution, and through the round rod 455, the stirring ring 456 is driven to disturb the solution up and down, which breaks the particle agglomeration, enhances the mass transfer, and improves the capture efficiency of benzothiazole.

[0041] Please refer to Figures 5-12 As shown, in the embodiment, the extrusion device 500 comprises a pair of racks 510, an inner gear 520 arranged between the pair of racks 510, and a piston plate 540 moving with the rear rack 510.

[0042] Specifically, the extrusion device 500 further comprises a sliding rod 530 clamped to the end of the transverse plate of the rear rack 510, and a second spring 550 sleeved outside the sliding rod 530, the two racks 510 are centrally symmetrically distributed, the inner gear 520 is engaged with the two racks 510, the size of the transverse plate at the bottom end of the front rack 510 is smaller than the size of the plate surface groove 251, the piston plate 540 is clamped to the bottom end of the sliding rod 530, and the elastic force provided by the second spring 550 pushes the rear rack 510 to move upward.

[0043] Further, the toothed plate 250 controlled by the electric push rod 240 also drives the front toothed plate 250 to move upward, engages the inner gear 520 to drive the rear rack 510 to move downward, so that the piston plate 540 flows out the solution in the SPE column 300 at a fixed flow rate, and the size of the pair of racks 510 and the inner gear 520 can be adjusted according to different steps to ensure that different solvents can flow through the SPE column at corresponding flow rates.

[0044] The application also provides an extraction method of benzothiazole compounds in ryegrass hydroponics solution, which uses the extraction device of benzothiazole compounds in ryegrass hydroponics solution, and comprises the following steps: S1, first, the operator filters the ryegrass hydroponics solution through a filter membrane, adjusts the pH value of the ryegrass hydroponics solution, and adds a deuterated benzothiazole internal standard into the adjusted ryegrass hydroponics solution to correct the recovery rate; S2, then, the operator first methanol, ultrapure water, treated ryegrass hydroponics solution, weak solvent, strong solvent from left to right into the interior of several liquid storage tube 400, and the SPE column 300 into the end of the slider 230 hole; S3, then, start the motor 210, drive the screw 220 rotation after the slider 230 transverse, and let the electric push rod 240 along with the tooth plate 250 transverse, the SPE column 300 to load with methanol liquid storage tube 400 below; S4, control electric push rod 240 drive tooth plate 250 up, located in the plate recess 251 in the top of the bending plate 440 transverse plate along with the same displacement, and drive the end of the rod 422 along the inclined slot 441 move, drive the insertion rod 420 in the cross pipe body 411 transverse, let the through hole 421 and the vertical pipe of cross pipe body 411 after the opening, methanol drop into the interior of the SPE column 300, S5, then, control electric push rod 240 let the tooth plate 250 and bending plate 440 reset, and start the motor 210, the SPE column 300 to the leftmost extrusion device 500 below; S6, control electric push rod 240 up again, the front side of the rack 510 up, through the meshing of the internal gear 520 drive rear side of the rack 510 down, and drive the slide bar 530 and its bottom end of the piston plate 540 down, let the methanol from the SPE column 300 drop at a fixed flow rate, so as to ensure the solid phase packing wet, open the bonding site, realize the activation of solid phase packing; S7, then, repeat the above operation, let the ultrapure water flow through the solid phase packing, replace the activated solvent, so that the solid phase packing internal environment and the treated ryegrass hydroponics solution consistent; S8, control motor 210 again drive the tooth plate 250 transverse, the tooth plate 250 will engage the gear 451, drive the shaft 452 rotation, and let the stirring blade 453 and its between the connecting rod 454 in the ryegrass hydroponics solution rotation, at the same time through the round bar 455, drive the stirring ring 456 up and down disturbance solution, and break the particle agglomeration, enhance the mass transfer, and improve the efficiency of benzothiazole capture; S9, then, the tooth plate 250 again up and down, the ryegrass hydroponics solution flow through the solid phase packing, its internal benzothiazole compound selective adsorption on the solid phase packing, and complete the sample operation; S10, then, first through the weak solvent elution of solid phase packing on the hydrophilic interferent, and then through the strong solvent benzothiazole compound from the solid phase packing; S11, finally, the operator takes the rightmost glass container 600, and send it into the nitrogen blowing instrument, rich in benzothiazole compound, and then save.

[0045] The foregoing description of specific exemplary embodiments of the application has been presented for the purposes of illustration and description. It is not intended to be exhaustive or to limit the application to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. It is intended that the scope of the application be limited not with the specific embodiments described above, but rather by the claims appended hereto.

Claims

1. A device for extracting benzothiazole compounds from a ryegrass hydroponic solution, comprising a hydroponic solution processing bin and an SPE column; characterized in that Further comprising a material conveying mechanism for conveying the SPE column, a plurality of liquid storage tubes arranged above the SPE column, and a squeezing device; The material conveying mechanism comprises an electric push rod and a toothed plate at the top end of the electric push rod; The liquid storage tube comprises a fixed tube, a plug rod arranged below the fixed tube, a bent plate sleeved on the outer end of the plug rod, and a stirring part arranged in the middle of the liquid storage tube; a cross tube is connected to the bottom end of the fixed tube, the plug rod slides in the cross tube, a through hole is formed in the plug rod, and a protruding rod is integrally formed at the end of the through hole; a slanted groove is formed in the bottom of the bent plate for the protruding rod to slide in; The upper end of the bent plate is slidingly connected to the side of the toothed plate, the electric push rod controls the toothed plate to move upward, the protruding rod moves along the slanted groove, the plug rod moves horizontally to make the through hole communicate with the vertical tube of the cross tube, and then the solution in the fixed tube falls into the SPE column; when the toothed plate moves horizontally, it also drives the stirring part to stir the solution in the middle of the liquid storage tube; The squeezing device comprises a pair of toothed racks, an internal gear arranged between the pair of toothed racks, and a piston plate moving with the toothed racks; the electric push rod controls the toothed plate to move upward, which drives the rear toothed rack to move downward through the internal gear, and then the piston plate flows the solution in the SPE column at a fixed flow rate.

2. The apparatus according to claim 1, wherein the benzothiazole compound is extracted from the hydroponic solution of rye grass. The hydroponic solution processing bin comprises a bin body, a fixed frame welded on the outer wall of the bin body, a partition plate arranged below the fixed frame and welded to the bin body, a plurality of limiting frames regularly connected to the outer wall of the bin body, a plurality of sealing plugs connected to the top surface of the bin body, and a hanging frame welded on the front wall of the bin body; a plurality of glass containers are regularly placed on the inner bottom surface of the bin body.

3. The apparatus according to claim 2, wherein: A sliding groove is formed in the bin body, a placing groove is formed in the front side of the sliding groove, a funnel is connected to the partition plate above the plurality of glass containers, the positions of the plurality of limiting frames correspond to the positions of the plurality of squeezing devices, and the plurality of sealing plugs are above the liquid storage tubes.

4. The apparatus according to claim 3, wherein the benzothiazole compound is extracted from the hydroponic solution of rye grass. The material conveying mechanism further comprises a motor, a screw rod coaxially connected to the output shaft of the motor, and a sliding block sleeved on the outer side of the screw rod and slidingly connected in the sliding groove and the placing groove; the electric push rod is fixedly connected to the top surface of the sliding block by screws; a plate recess is formed in the outer wall of the toothed plate, and the upper end of the bent plate is slidingly connected to the plate recess.

5. The apparatus according to claim 4, wherein the benzothiazole compound is extracted from the aqueous solution of rye grass. The SPE column comprises a column body inserted into the end of the horizontal plate of the sliding block, a solid-phase filler for capturing benzothiazole compounds arranged in the column body, and two filter screens connected to the inside of the column body; the two filter screens are arranged above and below the solid-phase filler.

6. The apparatus according to claim 5, wherein the benzothiazole compound is extracted from the rye grass hydroponic solution. The liquid storage tube further comprises a first spring adhered to the rear end of the plug rod; the fixed tube is connected to the inner bottom surface of the fixed frame; and the other end of the first spring is adhered to the wall of the cross tube.

7. The apparatus according to claim 6, wherein the benzothiazole compound is extracted from the rye grass hydroponic solution. The bent plate is slidingly connected to the inside of the limiting frame, the slanted groove is arranged with a higher front and a lower back, and the size of the top horizontal plate of the bent plate is smaller than the size of the plate recess.

8. The apparatus according to claim 7, wherein the benzothiazole compound is extracted from the hydroponic solution of rye grass. The stirring part comprises an outer gear, a rotating shaft clamped in the inner part of the outer gear, and two stirring blades rotating with the rotating shaft, further comprises a connecting rod connected between the two stirring blades, a round rod rotatingly connected to the outer side of the connecting rod, and a stirring ring rotatingly connected to the top end of the round rod, the stirring ring is slidingly connected to the inner part of the fixed pipe body, and the outer gear is engaged with the toothed plate.

9. The apparatus according to claim 8, wherein the benzothiazole compound is extracted from the hydroponic solution of rye grass. The extrusion device further comprises a sliding rod clamped at the end of the rear rack transverse plate and a second spring sleeved on the outer side of the sliding rod, the two racks are centrally symmetrically distributed, the inner gear is engaged with the two racks, the bottom end of the rack on the front side is smaller than the size of the plate surface groove, the piston plate is clamped at the bottom end of the sliding rod, and the second spring provides an elastic force to push the rear rack upward.

10. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to any one of claims 1-9, characterized in that, The method for extracting benzothiazole compounds in ryegrass hydroponics solution by using the device comprises the following steps: S1, first, the operator filters the ryegrass hydroponics solution through the filter membrane, adjusts the pH value of the ryegrass hydroponics solution, and adds a deuterated benzothiazole internal standard into the adjusted ryegrass hydroponics solution to correct the recovery rate; S2, then, the operator first fills methanol, ultrapure water, treated ryegrass hydroponics solution, weak solvent and strong solvent into the inner part of a plurality of liquid storage tubes from left to right, and places the SPE column into the circular hole at the end of the sliding block; S3, then, the motor is started to drive the screw rod to rotate and make the sliding block move horizontally, and the electric push rod moves horizontally together with the toothed plate, so that the SPE column is sent below the liquid storage tube filled with methanol; S4, the electric push rod is controlled to move upward with the toothed plate, the top transverse plate of the bent plate in the plate surface groove is displaced together, the convex rod at the end of the insertion rod is driven to move along the inclined groove, the insertion rod moves horizontally in the cross pipe body, the through hole is communicated with the vertical pipe of the cross pipe body, and then the methanol is dropped into the inner part of the SPE column, S5, then, the electric push rod is controlled to reset the toothed plate and the bent plate, and the motor is started to send the SPE column below the extrusion device on the leftmost side; S6, the electric push rod is controlled to move upward again, the rack on the front side moves upward, the rear rack moves downward through the engaged inner gear, the sliding rod and the piston plate at the bottom end of the sliding rod move downward, the methanol drops from the SPE column at a fixed flow rate, so as to ensure that the solid-phase filler is wetted, the bonding site is opened, and the activation of the solid-phase filler is realized; S7, then, the above operation is repeated, the ultrapure water flows through the solid-phase filler, replaces the activated solvent, and makes the internal environment of the solid-phase filler consistent with the treated ryegrass hydroponics solution; S8, during the process of controlling the motor to drive the toothed plate to move horizontally again, the toothed plate engages with the outer gear to drive the rotating shaft to rotate, the stirring blades and the connecting rod therebetween rotate in the ryegrass hydroponics solution, the stirring ring is driven to disturb the solution up and down through the round rod, the particle agglomeration is broken, the mass transfer is enhanced, and the benzothiazole capture efficiency is improved; S9, then, the toothed plate moves up and down again, the ryegrass hydroponics solution flows through the solid-phase filler, and the benzothiazole compounds in the ryegrass hydroponics solution are selectively adsorbed on the solid-phase filler, and the sample loading operation is completed; S10, then, the hydrophilic interferents on the solid-phase filler are eluted by the weak solvent, and the benzothiazole compounds are desorbed from the solid-phase filler by the strong solvent. S11. Finally, the operator takes the glass container on the far right and puts it into the nitrogen evaporator to enrich the benzothiazole compound, and then stores it.

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