3D (three-dimensional) rotary incubator suitable for preparing mesenchymal stem cells by digesting various tissues

Abstract

The embodiment of the invention provides a 3D rotary incubator suitable for preparing mesenchymal stem cells by digesting various tissues, and relates to the technical field of constant-temperature preservation of cells. A 3D rotary incubator suitable for preparing mesenchymal stem cells through digestion of various tissues comprises an incubator body, a cavity is formed in the lower-layer space of the incubator body, cavities used for storing the mesenchymal stem cells are formed in the upper-layer space of the incubator body in an axial symmetry mode, and sealing covers used for sealing the cavities are connected to the top of the incubator body in a clamped mode. A temperature probe for monitoring the temperature change in the cavity in real time and a grab handle head fixed on the sealing cover are embedded in the box body, and the bottom of the box body is fixedly connected with a bracket with a fixed plate. A group of reagent bottles or a plurality of groups of reagent bottles which are independently filled with stem cells are shaken in all directions according to requirements by adopting a mode of combining independent rotation and positioning, so that the layered precipitation phenomenon of the stem cells is eliminated; the purpose of balancing and adjusting the independent carbon-oxygen ratio in the chamber is achieved, and the activity of the mesenchymal stem cells is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cell constant-temperature preservation, and particularly relates to a 3D rotary constant-temperature box suitable for preparing mesenchymal stem cells from various tissues. BACKGROUND

[0002] Mesenchymal stem cells are a kind of multipotent stem cells, which have all the common properties of stem cells, i.e. self-renewal and multi-directional differentiation ability. Before the mesenchymal stem cells are delivered, the mesenchymal stem cells need to be contained in a container and then placed in a constant-temperature box for static preservation, so as to ensure the sample activity of the mesenchymal stem cells.

[0003] In the prior art (patent application with the patent name of a constant-temperature device for stem cell culture and the announcement number of CN219839729U), the temperature of each position in the constant-temperature box is more uniform and convenient for disinfection. In the process of realizing the technical scheme, it is found that at least the following problems exist in the prior art: During the preservation of the mesenchymal stem cells in the constant-temperature box, static preservation is mostly adopted, which easily leads to the stratified precipitation phenomenon of the tissues in the mesenchymal stem cells, is not conducive to the subsequent delivery and detection operation, and when the mesenchymal stem cells are accessed, the external air enters at will, which also causes the imbalance of oxygen and carbon dioxide in the constant-temperature box, and affects the activity of the mesenchymal stem cells. SUMMARY

[0004] The present application aims to at least solve one of the technical problems that the independent rotation and positioning combination cannot be adopted to shake a group of reagent bottles or multiple groups of reagent bottles independently containing stem cells in all directions as needed, and the stratified precipitation phenomenon occurs. To this end, the present application proposes a 3D rotary constant-temperature box suitable for preparing mesenchymal stem cells from various tissues.

[0005] To achieve the above-mentioned purpose, the specific technical scheme of the present application is as follows: The 3D rotary constant-temperature box suitable for preparing mesenchymal stem cells from various tissues comprises a box body, a cavity is opened in the lower space of the box body, an axis-symmetrical chamber for storing mesenchymal stem cells is opened in the upper space of the box body, a cover for plugging the chamber is clamped on the top of the box body, a temperature probe for monitoring the temperature change in the chamber in real time is embedded in the cover, a handle head is fixed on the cover, a bracket with a fixed plate is fixedly connected to the bottom of the box body, and a handle head is fixed on the cover. The inner side of the support is fixed with a supercharged cylinder for supercharged work and a negative pressure cylinder for negative pressure work, the outer side of the supercharged cylinder and the negative pressure cylinder is fixed with a blast stand matched with the support, and the blast stand is used as a wind pressure buffer space of the cold source and the heat source, the cavity is provided with a rotating assembly and a vibrating assembly for rotating and vibrating the mesenchymal stem cells stored in the cavity, and the outer side of the supercharged cylinder and the negative pressure cylinder is provided with a work assembly and a balancing assembly for balancing oxygen and carbon dioxide in the cavity. The rotating assembly comprises a double-head motor fixed on the bottom of the box body and a rotating shaft rotating in the middle of the cavity, the vibrating assembly comprises a vibrating motor embedded on the top of the rotating shaft and a vibrating head fixed thereon, the vibrating head is slidingly sleeved with the rotating shaft, the work assembly comprises a four-way valve communicated with the supercharged cylinder and the negative pressure cylinder, and the balancing assembly comprises a feed and discharge pipe communicated with the two ends of the four-way valve.

[0006] Preferably, the rotating assembly further comprises an upper main gear sleeved on one output shaft of the double-head motor, upper slave gears are arranged around the outer side of the upper main gear, first electric push rods are fixedly connected to the outer side of the upper slave gears, a worm is sleeved on the first electric push rods, and a turbine fixedly connected with the rotating shaft is engaged with the outer side of the worm.

[0007] Preferably, the vibrating assembly further comprises a sliding frame fixedly connected to the bottom of the vibrating head and slidingly sleeved with the rotating shaft, limit clamps are fixedly connected to the outer side of the sliding frame in an axisymmetric manner, the limit clamps located above are in an open state, the limit clamps located below are in a closed state at the bottom, reagent bottles containing mesenchymal stem cells are inserted into the limit clamps, clamping arms are hingedly connected around the vibrating head, and clamping heads for positioning the reagent bottles are fixedly connected to the bottom of the clamping arms.

[0008] Preferably, the work assembly further comprises a lower main gear sleeved on the other output shaft of the double-head motor, lower slave gears are arranged on both sides of the lower main gear, second electric push rods are fixedly connected to the outer side of the lower slave gears, a cam is fixedly connected to the outer side of the second electric push rods, a connecting rod is rotatably connected to the outer side of the cam, and pistons for supercharged work in the supercharged cylinder and negative pressure work in the negative pressure cylinder are hingedly connected to the top of the connecting rod.

[0009] Preferably, the balancing assembly further comprises a connecting head communicated with the bottom end of the feed and discharge pipe, oxygen storage tanks, carbon dioxide storage tanks and two groups of gas storage tanks are threadedly communicated with the bottom end of the connecting head, check valves are embedded in the openings of the oxygen storage tanks, the carbon dioxide storage tanks and the two groups of gas storage tanks, a gas supplement pipe for independently supplying oxygen and carbon dioxide is communicated with the outer end of the four-way valve located at the supercharged cylinder, and an air extraction pipe for air suction and storage in the cavity is communicated with the outer end of the four-way valve located at the negative pressure cylinder.

[0010] Preferably, the vibration head is embedded with compression springs around the periphery of the clamping arm, and the ends of the compression springs are fixedly connected with the clamping arm, and a clamping groove is formed on the stopper of the reagent bottle for clamping the clamping head, the inner end of the four-way valve is embedded with a flow sensor for detecting the amount of oxygen, carbon dioxide and air, and the concentration sensors are embedded at the supply and discharge ports of the oxygen storage tank, the carbon dioxide storage tank and the two groups of air storage tanks.

[0011] Preferably, the oxygen sensor and the carbon dioxide sensor are embedded on the outer side of the chamber of the box body for detecting the amount of oxygen and carbon dioxide in the chamber, and the air supply cover and the air exhaust cover are fixedly connected with the four corners of the box body and communicate with the chamber.

[0012] Preferably, the air supply covers are communicated through air supply straight pipes, the air exhaust covers are communicated through air exhaust straight pipes, and the air supply straight pipes and the air exhaust straight pipes are designed with one-way valves to prevent backflow.

[0013] Preferably, the flow uniformizing plates are embedded on the side of the box body close to the air supply cover and the air exhaust cover, the filter frames are embedded on the inner side of the air exhaust cover, and the filter screens are fixed on the inner and outer sides of the filter frames.

[0014] Preferably, the filter frames are filled with molecular sieves blocked by the filter screens, and the embedded electric heating sheets are embedded on the two sides of the filter frames for heating and slow release of the molecular sieves.

[0015] The 3D rotary thermostat suitable for preparing mesenchymal stem cells from various tissues has the following advantages: 1. The 3D rotary thermostat suitable for preparing mesenchymal stem cells from various tissues independently controls the meshing stroke adjustment between the upper main gear and one or more upper from gears through the first electric push rod according to the actual needs of preserving mesenchymal stem cells in the chamber, then controls the rotation of the one or more upper from gears through the upper main gear by the double-head motor, and correspondingly drives the one or more worms to rotate, the worm in the rotating state drives the shaft on the turbine to rotate linearly, at the same time, the vibration motor in the rotating state is controlled to be turned on and drives the sliding frame through the vibration head, the mesenchymal stem cells in the reagent bottle clamped and positioned by the limiting clamp are rotated and oscillated at the same time, and the reagent bottle in the limiting clamp is positioned and pressed down by the clamping head on the clamping arm, so that the stratified sedimentation phenomenon of the mesenchymal stem cells in the reagent bottle caused by gravity is eliminated, the mesenchymal stem cells in the reagent bottle are conveniently and quickly sent for inspection, and the mesenchymal stem cells in the reagent bottle do not need to be shaken evenly in the inspection link, so that the efficiency is high.

[0016] 2、The 3D rotary incubator suitable for preparing mesenchymal stem cells from various tissue digestion, then, the second electric push rod at the booster cylinder and the negative pressure cylinder is controlled to adjust the meshing stroke between the lower main gear and the two lower pinion gears, then the double-head motor rotates the lower pinion gears at the booster cylinder or the negative pressure cylinder through the lower main gear, then the rotating lower pinion gears drive the pistons on the connecting rods to do work in the booster cylinder or the negative pressure cylinder, and the booster pressure or the negative pressure generated in the booster cylinder or the negative pressure cylinder is applied to the oxygen storage tank, the carbon storage tank and the two groups of gas storage tanks through the four-way valve, the charging pipe and the adapter, then the oxygen in the oxygen storage tank or the carbon dioxide in the carbon storage tank is independently supplemented into the chamber through the charging pipe according to the carbon-oxygen ratio in the chamber, and the excessive carbon-oxygen air in the chamber is independently extracted into the two groups of gas storage tanks through the exhaust pipe, so as to balance and adjust the independent carbon-oxygen ratio in the chamber and improve the activity of mesenchymal stem cells.

[0017] 3、The 3D rotary incubator suitable for preparing mesenchymal stem cells from various tissue digestion, during which, the impellers on the two groups of differential gears are continuously rotated in the two groups of air blowing frames by the double-head motor through the lower main gear according to the actual needs of preserving mesenchymal stem cells in the chamber, at the same time, the cold and heat integrated machine provides cold or heat supply according to the temperature change in the chamber, and the cold or heat supply is supplied into the two manifolds through the two angle pipes, then the air pressure in the two groups of air blowing frames independently supplies the cold or heat in the two manifolds into the chamber where the mesenchymal stem cells are preserved through the delivery pipe opened by the electric control valve, and the chamber where the mesenchymal stem cells are not preserved is cut off, so as to independently supply the cold and heat source in an energy-saving mode under the premise of ensuring the constant temperature preservation needs of the mesenchymal stem cells. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0019] Figure 1 The structure schematic diagram of the 3D rotary incubator suitable for preparing mesenchymal stem cells from various tissue digestion of the present application; Figure 2 The structure bottom view of the 3D rotary incubator suitable for preparing mesenchymal stem cells from various tissue digestion of the present application; Figure 3 The structure sectional view of the 3D rotary incubator suitable for preparing mesenchymal stem cells from various tissue digestion of the present application; Figure 4 Structure of the 3D rotating incubator for the present invention, which is suitable for the preparation of mesenchymal stem cells by digesting various tissues; Figure 5 Structure of the rotating assembly and the oscillating assembly of the present invention, front view; Figure 6 Structure of the rotating assembly and the oscillating assembly of the present invention, bottom view; Figure 7 Structure of the rotating assembly of the present invention, top view; Figure 8 Structure of the rotating assembly and the oscillating assembly of the present invention, partial exploded view; Figure 9 Structure of the rotating assembly of the present invention, partial cross-sectional view; Figure 10 Structure of the oscillating assembly of the present invention, partial exploded view; Figure 11 Structure of the pressure cylinder, the negative pressure cylinder, the working assembly and the balancing assembly of the present invention, front view; Figure 12 Structure of the pressure cylinder, the working assembly and the balancing assembly of the present invention, bottom view, in which oxygen and carbon dioxide are supplied independently in the chamber; Figure 13 Structure of the negative pressure cylinder, the working assembly and the balancing assembly of the present invention, bottom view, in which oxygen and carbon dioxide are sucked independently in the chamber; Figure 14 Structure of the pressure cylinder, the negative pressure cylinder and the working assembly of the present invention, cross-sectional view; Figure 15 Structure of the filter frame of the present invention, exploded view; Figure 16 Structure of the box and the cover of the present invention, exploded cross-sectional view; Figure 17 Structure of the air blowing frame and the temperature control assembly of the present invention, side view; Figure 18 Structure of the air blowing frame and the temperature control assembly of the present invention, cross-sectional view.

[0020] The mark in the figure is explained: 1, box; 2, chamber; 3, cover; 4, support; 5, booster cylinder; 6, negative pressure cylinder; 7, air blowing frame; 81, double-head motor; 82, upper main gear; 83, upper from gear; 84, first electric push rod; 85, worm; 86, turbine; 87, rotating shaft; 91, vibration motor; 92, vibration head; 93, sliding frame; 94, limit clamp; 95, reagent bottle; 96, clamping arm; 97, chuck; 101, lower main gear; 102, lower from gear; 103, second electric push rod; 104, cam; 105, connecting rod; 106, piston; 107, four-way valve; 111, feeding and discharging pipe; 112, adapter; 113, oxygen storage tank; 114, carbon storage tank; 115, gas storage tank; 116, air supplement pipe; 117, air exhaust pipe; 121, differential gear; 122, impeller; 123, manifold; 124, angle pipe; 125, cold and hot integrated machine; 126, conveying pipe; 127, electric control valve; 13, compression spring; 14, flow sensor; 15, concentration sensor; 16, oxygen sensor; 17, carbon dioxide sensor; 18, air supplement cover; 19, air supplement straight pipe; 20, air exhaust cover; 21, air exhaust straight pipe; 22, check valve; 23, flow equalizing plate; 24, filter frame; 25, filter screen; 26, molecular sieve; 27, embedded electric heating sheet. DETAILED DESCRIPTION

[0021] The application will be specifically introduced below in combination with the drawings and specific examples: As Figures 1-18 shown, the 3D rotary thermostat suitable for preparing mesenchymal stem cells by digesting various tissues of the application comprises a box 1, a cavity is formed in the lower space of the box 1, and an axisymmetric chamber 2 for storing mesenchymal stem cells is formed in the upper space of the box 1, a cover 3 for plugging the chamber 2 is clamped on the top of the box 1, and a temperature probe for monitoring the temperature change in the chamber 2 in real time is embedded in the cover 3, the temperature change in the chamber 2 under the cover 3 is monitored in real time, so as to provide data reference for subsequent constant temperature control, and a handle head is fixed on the cover 3, and a support 4 with a fixed plate is fixedly connected to the bottom of the box 1; the inner side of the support 4 is fixedly provided with a booster cylinder 5 for boosting work and a negative pressure cylinder 6 for negative pressure work, the outer side of the booster cylinder 5 and the negative pressure cylinder 6 are alternately fixed with air blowing frames 7 fixedly matched with the support 4, and the air blowing frames 7 are used as air pressure buffer spaces of cold source and heat source; And the cavity is provided with a rotating assembly and a vibrating assembly for rotating and vibrating the mesenchymal stem cells stored in the chamber 2, the rotating assembly comprises a double-head motor 81 fixed on the bottom of the box 1 and a rotating shaft 87 rotating in the middle of the chamber 2, and the vibrating assembly comprises a vibrating motor 91 embedded on the top of the rotating shaft 87 and a vibrating head 92 fixed thereon, and the vibrating head 92 is slidingly sleeved with the rotating shaft 87, so as to eliminate the stratified precipitation of the mesenchymal stem cells in the reagent bottle 95 caused by gravity, and facilitate the subsequent rapid inspection of the mesenchymal stem cells in the reagent bottle 95; And the chamber 2 is provided with a working assembly and a balancing assembly for balancing the oxygen and carbon dioxide in the chamber 2; the working assembly comprises a four-way valve 107 communicated with the positive pressure cylinder 5 and the negative pressure cylinder 6, and the balancing assembly comprises a feed and discharge pipe 111 communicated with both ends of the four-way valve 107, so that the carbon-oxygen air exceeding the standard in the chamber 2 is independently extracted into the two groups of gas storage tanks 115 through the exhaust pipe 117, so as to achieve the purpose of independent carbon-oxygen ratio balance adjustment in the chamber 2, and improve the activity of the mesenchymal stem cells.

[0022] As shown in Figures 5-16 The rotating assembly further comprises an upper main gear 82 sleeved on one output shaft of the double-head motor 81, and the outer side of the upper main gear 82 is provided with an upper slave gear 83 around, the engagement stroke between the upper main gear 82 and one or more upper slave gears 83 is independently adjusted to the position by the first electric push rod 84, the double-head motor 81 is controlled to rotate one or more upper slave gears 83 through the upper main gear 82, and the outer side of the upper slave gear 83 is fixedly connected with the first electric push rod 84, and the first electric push rod 84 is sleeved with a worm 85 matched with the chamber 2 in rotation, the rotating state of the upper slave gear 83 drives one or more worms 85 to rotate correspondingly, the outer side of the worm 85 is engaged with a turbine 86 fixedly matched with the rotating shaft 87, and the rotating state of the worm 85 drives the rotating shaft 87 on the turbine 86 to rotate linearly; The oscillation assembly further comprises a sliding frame 93 fixedly connected to the bottom of the vibration head 92 and sleeved with the rotating shaft 87, corresponding control of the vibration motor 91 in the rotating state to open and drive the sliding frame 93 through the vibration head 92, and the outer side of the sliding frame 93 is symmetrically fixed with a limiting clamp 94, the limiting clamp 94 at the top is in an open state, the limiting clamp 94 at the bottom is in a closed state, and a reagent bottle 95 containing mesenchymal stem cells is inserted into the limiting clamp 94, the mesenchymal stem cells in the reagent bottle 95 clamped and positioned by the limiting clamp 94 are oscillated while rotating, eliminating the stratified precipitation phenomenon of the mesenchymal stem cells in the reagent bottle 95 caused by gravity, the four perimeters of the vibration head 92 are hingedly connected with clamping arms 96, the bottom of each clamping arm 96 is fixedly connected with a clamping head 97 for positioning the reagent bottle 95, the four perimeters of the vibration head 92 close to the clamping arms 96 are embedded with compression springs 13, the clamping arms 96 are elastically supported, the clamping arms 96 drive the clamping heads 97 to be clamped downward, the ends of the compression springs 13 are fixedly connected with the clamping arms 96, and a clamping groove for clamping the clamping head 97 is formed in the stopper of the reagent bottle 95, the clamping head 97 is driven downward by the clamping arms 96 and the compression springs 13 to be clamped into the clamping groove on the stopper of the reagent bottle 95, and the reagent bottle 95 is pressed downward to improve the positioning stability of the reagent bottle 95 in the limiting clamp 94.

[0023] The work assembly further comprises a lower main gear 101 sleeved on the other output shaft of the double-head motor 81, lower from gears 102 are arranged on both sides of the lower main gear 101, second electric push rods 103 are fixedly connected to the outer sides of the lower from gears 102, the second electric push rods 103 located at the pressurizing cylinder 5 and the negative pressure cylinder 6 are controlled to correspondingly adjust the meshing stroke between the lower main gear 101 and the two lower from gears 102, the double-head motor 81 is controlled to rotate the lower from gears 102 at the pressurizing cylinder 5 or the negative pressure cylinder 6 through the lower main gear 101, cams 104 are fixedly connected to the outer sides of the second electric push rods 103, connecting rods 105 are rotatably connected to the outer sides of the cams 104, pistons 106 for pressurizing work in the pressurizing cylinder 5 and negative pressure work in the negative pressure cylinder 6 are hingedly connected to the top of the connecting rods 105, the pistons 106 on the connecting rods 105 are driven by the cams 104 corresponding to the lower from gears 102 in the rotating state to perform pressurizing work in the pressurizing cylinder 5 or negative pressure work in the negative pressure cylinder 6, and the pressurizing pressure force generated in the pressurizing cylinder 5 or the negative pressure pressure force generated in the negative pressure cylinder 6, the inner end of the four-way valve 107 is embedded with a flow sensor 14 for detecting the amount of oxygen, carbon dioxide and air, the amount of oxygen, carbon dioxide and air flowing through the four-way valve 107 is quantitatively monitored, and the concentration sensor 15 is embedded at the feed and discharge ports of the oxygen storage tank 113, the carbon storage tank 114 and the two groups of air storage tanks 115, the oxygen, carbon dioxide and air concentration in the oxygen storage tank 113, the carbon storage tank 114 and the two groups of air storage tanks 115 are monitored in real time, so as to be replaced in time; The balance assembly further comprises a connector 112 communicated with the bottom end of the feed and discharge pipe 111, and the bottom end of the connector 112 is respectively threaded communicated with an oxygen storage tank 113, a carbon dioxide storage tank 114 and two groups of gas storage tanks 115, and the openings of the oxygen storage tank 113, the carbon dioxide storage tank 114 and the two groups of gas storage tanks 115 are respectively embedded with check valves. Under the action of the supercharged pressure force generated in the supercharged cylinder 5 and the negative pressure force generated in the negative pressure cylinder 6, oxygen and carbon dioxide are independently supplied to the chamber 2 through the feed and discharge pipe 111 and the connector 112, and the outer end of the four-way valve 107 located at the supercharged cylinder 5 is communicated with a gas supplement pipe 116 for independently supplying oxygen and carbon dioxide, and the outer end of the four-way valve 107 located at the negative pressure cylinder 6 is communicated with an air extraction pipe 117 for air suction and storage in the chamber 2. According to the carbon-oxygen ratio in the chamber 2, the oxygen in the oxygen storage tank 113 or the carbon dioxide in the carbon dioxide storage tank 114 is independently supplemented into the chamber 2 through the gas supplement pipe 116, and the excessive carbon-oxygen air in the chamber 2 is independently extracted into the two groups of gas storage tanks 115 through the air extraction pipe 117, so as to achieve the purpose of independently balancing and adjusting the carbon-oxygen ratio in the chamber 2, improve the activity of mesenchymal stem cells, and the outer side of the box body 1 close to the chamber 2 is respectively embedded with an oxygen sensor 16 and a carbon dioxide sensor 17 for detecting the amount of oxygen and carbon dioxide therein, so as to monitor the carbon-oxygen ratio in the chamber 2 in real time under the use state. The four corners of the box body 1 are respectively fixedly connected with a gas supplement cover 18 and an air extraction cover 20 communicated with the chamber 2, the gas supplement cover 18 is communicated through a gas supplement direct pipe 19, the air extraction cover 20 is communicated through an air extraction direct pipe 21, and the gas supplement direct pipe 19 and the air extraction direct pipe 21 are designed with one-way valves 22 to prevent backflow, so as to separately transport and supplement oxygen and carbon dioxide, and extract air in the chamber 2 under the use state. The box body 1 close to the gas supplement cover 18 and the air extraction cover 20 is embedded with a flow equalization plate 23, which equalizes the flow of the gas passing through the gas supplement cover 18 and the air extraction cover 20 to prevent turbulence, and the inner side of the air extraction cover 20 is embedded with a filter frame 24, and the filter net 25 is fixed on the inner and outer sides of the filter frame 24, which filters the dust and impurities mixed in the gas passing through the filter frame 24, the filter frame 24 is filled with molecular sieve 26 blocked by the filter net 25, which adsorbs carbon dioxide in the gas passing through the molecular sieve 26, and the both sides of the filter frame 24 are embedded with embedded electric heating sheets 27 for heating and releasing the molecular sieve 26, which controls the heating of the embedded electric heating sheets 27 to the molecular sieve 26 to force the release of the adsorbed carbon dioxide, thereby saving the cost of carbon dioxide.

[0024] As Figures 17-18As shown, the mesenchymal stem cells are kept in the constant temperature box during the storage period, and the mesenchymal stem cells inside are kept at a constant temperature, and the space without mesenchymal stem cells is wasted, the constant temperature supply resources are wasted, the constant temperature supply cost is increased, and the constant temperature supply effect of the space with mesenchymal stem cells is affected. Each chamber 2 is independently provided with a temperature control assembly, and the temperature control assembly comprises a differential gear 121 engaged on the other two sides of the lower main gear 101. The outer side of the differential gear 121 is fixedly connected with an impeller 122 matched with the air blowing frame 7 through a fan shaft. The double-head motor 81 drives the impellers 122 on the two sets of differential gears 121 to continuously rotate in the two sets of air blowing frames 7 through the lower main gear 101, and generates air pressure in the two sets of air blowing frames 7. The outer side of the air blowing frame 7 is communicated with a manifold 123, and the bottom of the manifold 123 is unidirectionally communicated with a cold and hot all-in-one machine 125 fixed in the fixed plate in the support 4 through an angle pipe 124. According to the temperature change in the chamber 2, the cold and hot all-in-one machine 125 is controlled to provide cold source or heat source supply, and the supplied cold source or heat source is supplied into the two manifolds 123 through the two angle pipes 124. The manifold 123 is communicated with a conveying pipe 126 with an electric control valve 127, and is communicated with the chamber 2 in the box body 1. The conveying pipe 126 and the chamber 2 are communicated with uniform flow holes in the circumferential direction. The air pressure in the two sets of air blowing frames 7 drives the cold source or heat source reaching the two manifolds 123 to pass through the conveying pipe 126 opened by the electric control valve 127, and is independently supplied into the chamber 2 storing mesenchymal stem cells. The chamber 2 without storing mesenchymal stem cells is disconnected. Under the premise of ensuring the constant temperature storage requirement of mesenchymal stem cells, the energy-saving independent supply of cold and heat sources is realized.

[0025] The working principle of the 3D rotating incubator suitable for preparing mesenchymal stem cells by digesting various tissues is as follows: firstly, one or more reagent bottles 95 containing mesenchymal stem cells are inserted into the limiting clamps 94 in the cavity 2, the clamping arms 96 are loosened, and the clamping heads 97 are forced to be clamped into the clamping grooves on the bottle stoppers of the reagent bottles 95 under the restoring force of the compression springs 13. Then, the cover 3 is tightly covered on the cavity 2 containing mesenchymal stem cells. According to the condition of the cavity 2 containing mesenchymal stem cells, one or more first electric push rods 84 at the cavity 2 containing mesenchymal stem cells are controlled to be opened and to drive the upper from gears 83 to be disengaged from the meshing part of the upper main gear 82. Then, the double-head motor 81 is controlled to be opened and to drive the worm 85 on the upper from gear 83 to rotate through the meshed upper main gear 82. The rotating worm 85 drives the rotating shaft 87 on the turbine 86 to rotate. The rotating shaft 87 drives the vibration head 92 to rotate linearly and vibrate at the same time through the opened vibration motor 91. The reagent bottles 95 in the limiting clamps 94 are rotated and vibrated through the sliding frame 93. The mesenchymal stem cells in the reagent bottles 95 in one or more cavities 2 are subjected to rotating, shaking and vibrating operations. Then, the double-head motor 81 is controlled to be paused, and one or more first electric push rods 84 at the cavity 2 containing mesenchymal stem cells are controlled to be closed and to drive the upper from gears 83 to be disengaged from the meshing part of the upper main gear 82 to the initial position. Meanwhile, the oxygen and carbon dioxide in the one or more chambers 2 in use are monitored in real time by the oxygen sensor 16 and the carbon dioxide sensor 17 on the one or more chambers 2 in use, and if the carbon oxygen imbalance occurs in the one or more chambers 2 in use, the second electric push rod 103 at the negative pressure cylinder 6 is first controlled to open and drive the lower gear 102 to mesh with the lower main gear 101, then the double-head motor 81 is controlled to reopen and drive the lower main gear 101 meshed in place at the negative pressure cylinder 6 to rotate through the lower gear 102 meshed in place, then the rotating lower main gear 101 drives the piston 106 on the connecting rod 105 to perform negative pressure work in the negative pressure cylinder 6 through the cam 104, and the negative pressure generated in the negative pressure cylinder 6 is transmitted to the suction pipe 117 by the four-way valve 107, then under the action of the negative pressure, the air in the chamber 2 in use is forced to be sucked by the suction straight pipe 21 through the suction hood 20, the amount ratio of oxygen and carbon dioxide in the air in the chamber 2 in use is reduced, the sucked air is detected by the flow sensor 14 after passing through the four-way valve 107 on the negative pressure cylinder 6, then the air is sequentially sucked into the two groups of gas storage tanks 115 through the adapter 112 on the two pipes 111 at the negative pressure cylinder 6, and the concentration of the air sucked into the two groups of gas storage tanks 115 is monitored in real time by the two groups of concentration sensors 15, so as to replace the gas storage tank 115 in time; After the air suction in the chamber 2 in the standby state is completed, the double-head motor 81 is first controlled to pause, and then the second electric push rod 103 at the negative pressure cylinder 6 is controlled to open and correspondingly drive the lower gear 102 to the meshing part of the lower main gear 101 to the initial position, and then the second electric push rod 103 at the pressure boosting cylinder 5 is controlled to open and correspondingly drive the lower gear 102 to the meshing part of the lower main gear 101, and then the double-head motor 81 is controlled to restart and drive the lower main gear 101 to rotate through the meshed lower gear 102, and then the lower main gear 101 in the rotating state drives the piston 106 on the connecting rod 105 in the pressure boosting cylinder 5 to do work through the cam 104, and the boosting pressure force generated in the pressure boosting cylinder 5 is transmitted by the four-way valve 107 through the adapter 112 on the feed and discharge pipe 111 to quantitatively supply the oxygen in the oxygen storage tank 113 into the air supplement pipe 116, and the oxygen flow through the four-way valve 107 is monitored in real time by the flow sensor 14 at the pressure boosting cylinder 5, and the oxygen concentration in the oxygen storage tank 113 is monitored in real time by the concentration sensor 15, so as to supplement the oxygen in time, and then the quantitative oxygen supplied into the air supplement pipe 116 is transmitted by the one-way valve 22 to the chamber 2 in the using state through the air supplement cover 18 and the air supplement straight pipe 19, and when the oxygen in the chamber 2 in the using state reaches the predetermined amount, the boosting pressure force generated in the pressure boosting cylinder 5 is transmitted by the four-way valve 107 through the adapter 112 on the feed and discharge pipe 111 to quantitatively supply the carbon dioxide in the carbon dioxide storage tank 114 into the air supplement pipe 116, and then the quantitative carbon dioxide is transmitted by the one-way valve 22 to the chamber 2 in the using state through the air supplement cover 18 and the air supplement straight pipe 19, until the carbon-oxygen ratio in the chamber 2 in the using state returns to the standard, and the activity of the mesenchymal stem cells in the reagent bottle 95 in the chamber 2 in the using state is improved, and then the double-head motor 81 is controlled to pause, and then the second electric push rod 103 at the pressure boosting cylinder 5 is controlled to close and correspondingly drive the lower gear 102 to disengage from the meshing part of the lower main gear 101 to the initial position; During this period, the air, oxygen and carbon dioxide in the chamber 2 in use are evenly flowed and filtered by the flow equalization plate 23 and the filter screen 25 on the filter frame 24, removing the air, oxygen, carbon dioxide and dust impurities mixed in the chamber 2, at the same time, the carbon dioxide in the air in the chamber 2 in use is adsorbed by the molecular sieve 26 in the filter frame 24, so that the oxygen in the suction air can enter the two groups of gas storage tanks 115 to the maximum extent, providing standby for subsequent oxygen supplement, saving oxygen cost, and when the chamber 2 after air suction needs to be supplemented with carbon dioxide, the embedded electric heating sheet 27 in the filter frame 24 can be controlled to be turned on to heat the molecular sieve 26, and then the adsorbed carbon dioxide in the molecular sieve 26 is heated and released to flow back to the chamber 2 needing to be supplemented with carbon dioxide, saving the cost of carbon dioxide. This process is monitored by the oxygen sensor 16 and the carbon dioxide sensor 17 in real time until the carbon-oxygen ratio in the chamber 2 in use reaches the preset carbon-oxygen ratio set value of the oxygen sensor 16 and the carbon dioxide sensor 17. At the same time, the temperature in the chamber 2 in use is monitored in real time by the temperature probe on the tightly covered cover 3, the double-head motor 81 is continuously turned on, the impeller 122 on the two groups of differential gears 121 is driven to rotate linearly in the two groups of air blowing frames 7 through the lower main gear 101, and at the same time, wind pressure is generated in the two groups of air blowing frames 7. According to the temperature change of the chamber 2 in use monitored by the temperature probe, if the temperature is low, the cold-heat all-in-one machine 125 generates heat source supply, which is supplied into the manifold 123 through the angle pipe 124 in one direction, if the temperature is high, the cold-heat all-in-one machine 125 generates heat source supply, which is also supplied into the manifold 123 through the angle pipe 124 in one direction. The electric control valve 127 on the conveying pipe 126 at the chamber 2 in use is in an open state, and the heat source or cold source supplied into the manifold 123 is correspondingly supplied into the conveying pipe 126 at the same time. The wind pressure generated in the two groups of air blowing frames 7 is also correspondingly supplied into the conveying pipe 126. Under the action of the wind pressure, the heat source or cold source supplied is correspondingly forced to uniformly reach the chamber 2 in use through the flow holes on the conveying pipe 126. The electric control valve 127 on the conveying pipe 126 at the chamber 2 not in use is in a closed state, stopping the supply of heat source or cold source, until the temperature in the chamber 2 in use reaches the constant temperature set value of the temperature probe. The mesenchymal stem cells in the reagent bottle 95 in the chamber 2 in use are stored at constant temperature.

[0026] It should be noted that the specific model and specification of the double-head motor 81, the electric push rod, the vibration motor 91, the cold-heat all-in-one machine 125, the embedded electric heating sheet 27 and various valves and sensors need to be determined according to the actual specification of the device. The specific selection calculation method adopts the existing technology in the art, so it will not be described in detail.

[0027] The power supply circuits of the double-head motor 81, the electric push rod, the vibration motor 91, the cold and hot integrated machine 125, the embedded electric heating sheet 27, and various valves and sensors are clear to those skilled in the art, and will not be described in detail here.

[0028] It can be understood that the present application is described by some embodiments, and those skilled in the art know that various changes or equivalent replacements can be made to these features and embodiments without departing from the spirit and scope of the present application. In addition, under the guidance of the present application, modifications can be made to these features and embodiments to adapt to specific conditions and materials without departing from the spirit and scope of the present application. Therefore, the present application is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of the present application are within the scope of the present application.

Claims

1. A 3D rotating thermostat suitable for preparing mesenchymal stem cells by digesting various tissues, comprising a chamber (1), characterized in that: The lower space of the box (1) is provided with a cavity, and the upper space is provided with a chamber (2) for storing mesenchymal stem cells. The top of the box (1) is fitted with a cover (3) for sealing the chamber (2), and a temperature probe for real-time monitoring of temperature changes in the chamber (2) is embedded therein, as well as a handle head fixed on the cover (3). The bottom of the box (1) is fixedly connected with a bracket (4) with a fixing plate. The inner side of the support (4) is respectively fixed with a booster cylinder (5) for boosting work and a negative pressure cylinder (6) for negative pressure work. The outer sides of the booster cylinder (5) and the negative pressure cylinder (6) are respectively fixed with a blower frame (7) that is fixedly matched with the support (4) and used as a wind pressure buffer space for cold source and heat source. The cavity is provided with a rotating component and an oscillation component for rotating and oscillating mesenchymal stem cells stored in the chamber (2). The booster cylinder (5) and the negative pressure cylinder (6) are provided with a work component and a balance component for balancing oxygen and carbon dioxide in the chamber (2). The rotating assembly includes a dual-head motor (81) fixed to the bottom of the housing (1) and a rotating shaft (87) rotating in the middle of the chamber (2). The oscillation assembly includes a vibration motor (91) embedded in the top of the rotating shaft (87) and a vibration head (92) fixed thereon. The vibration head (92) is slidably sleeved with the rotating shaft (87). The working assembly includes a four-way valve (107) connected to the booster cylinder (5) and the negative pressure cylinder (6). The balancing assembly includes a supply pipe (111) connected to both ends of the four-way valve (107).

2. The 3D rotating thermostat chamber for preparing mesenchymal stem cells by digestion of various tissues according to claim 1, characterized in that: The rotating assembly also includes an upper master gear (82) sleeved on one output shaft of a dual-head motor (81), and upper driven gears (83) are provided around the outer side of the upper master gear (82). A first electric push rod (84) is fixedly connected to the outer side of the upper driven gear (83), and a worm gear (85) that rotates with the chamber (2) is sleeved on the first electric push rod (84). A turbine (86) that is fixedly engaged with the rotating shaft (87) is meshed on the outer side of the worm gear (85).

3. The 3D rotating thermostat chamber for preparing mesenchymal stem cells by digestion of various tissues according to claim 2, characterized in that: The oscillation assembly also includes a sliding frame (93) fixedly connected to the bottom of the vibrating head (92) and slidably sleeved with the rotating shaft (87), and a limiting clip (94) fixedly fixed on the outer side of the sliding frame (93) with the upper limiting clip (94) in an open state and the bottom of the lower limiting clip (94) in a closed state, and a reagent bottle (95) containing mesenchymal stem cells inserted into the limiting clip (94). The vibrating head (92) is hinged with a locking arm (96) around its perimeter, and a locking head (97) for positioning the reagent bottle (95) is fixedly connected to the bottom of the locking arm (96).

4. The 3D rotating thermostat for preparing mesenchymal stem cells by digestion of various tissues according to claim 3, characterized in that: The power-operating component also includes a lower master gear (101) sleeved on another output shaft of the dual-head motor (81), and a lower slave gear (102) is provided on both sides of the lower master gear (101). A second electric push rod (103) is fixedly connected to the outer side of the lower slave gear (102), and a cam (104) is fixedly connected to the outer side of the second electric push rod (103). A connecting rod (105) is rotatably connected to the outer side of the cam (104), and a piston (106) is hinged to the top of the connecting rod (105) to perform work under pressure in the booster cylinder (5) and under negative pressure in the negative pressure cylinder (6).

5. The 3D rotating thermostat chamber for preparing mesenchymal stem cells by digestion of various tissues according to claim 4, characterized in that: The balancing assembly also includes a connector (112) connected to the bottom end of the supply pipe (111), and the bottom end of the connector (112) is threadedly connected to an oxygen storage tank (113), a carbon storage tank (114) and two sets of gas storage tanks (115), and the openings of the oxygen storage tank (113), the carbon storage tank (114) and the two sets of gas storage tanks (115) are all embedded with check valves, and the outer end of the four-way valve (107) located at the booster cylinder (5) is connected to a supplementary air pipe (116) for independently supplying oxygen and carbon dioxide, and the outer end of the four-way valve (107) located at the negative pressure cylinder (6) is connected to an exhaust pipe (117) for sucking and storing air in the chamber (2).

6. The 3D rotating thermostat for preparing mesenchymal stem cells by digestion of various tissues according to claim 5, characterized in that: The vibrating head (92) is surrounded by compression springs (13) near the clamping arm (96), and the end of the compression spring (13) is fixedly connected to the clamping arm (96). The stopper of the reagent bottle (95) is provided with a groove for engaging with the clamping head (97). The inner end of the four-way valve (107) is provided with a flow sensor (14) for detecting the amount of oxygen, carbon dioxide and air. The oxygen storage tank (113), carbon storage tank (114) and the two sets of gas storage tanks (115) are all provided with concentration sensors (15).

7. The 3D rotating thermostat chamber for preparing mesenchymal stem cells by digestion of various tissues according to claim 6, characterized in that: The outer side of the box (1) near the chamber (2) is respectively equipped with an oxygen sensor (16) and a carbon dioxide sensor (17) for detecting the amount of oxygen and carbon dioxide inside. The four corners of the box (1) are respectively fixedly connected with an air supply hood (18) and an air extraction hood (20) that communicate with and cooperate with the chamber (2).

8. The 3D rotating thermostat for preparing mesenchymal stem cells by digestion of various tissues according to claim 7, characterized in that: The air supply hoods (18) are interconnected through the air supply straight pipe (19), and the air extraction hoods (20) are interconnected through the air extraction straight pipe (21). Both the air supply straight pipe (19) and the air extraction straight pipe (21) are designed with a one-way valve (22) to prevent backflow.

9. The 3D rotating thermostat chamber for preparing mesenchymal stem cells by digestion of various tissues according to claim 8, characterized in that: The box (1) has a flow equalization plate (23) embedded on the side near the air supply hood (18) and the air extraction hood (20), and a filter frame (24) is embedded on the inner side of the air extraction hood (20), as well as filter screens (25) fixed on the inner and outer sides of the filter frame (24).

10. The 3D rotating thermostat for preparing mesenchymal stem cells by digestion of various tissues according to claim 9, characterized in that: The filter frame (24) is filled with a molecular sieve (26) sealed by a filter screen (25), and both sides of the filter frame (24) are embedded with embedded electric heating elements (27) for heating and slow-release of the molecular sieve (26).

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