Angle-adjustable rotary tank type mesenchymal stem cell balling device

Abstract

The utility model discloses an angle-adjustable rotary tank type mesenchymal stem cell balling device, which relates to the technical field of mesenchymal stem cell balling, is arranged in a temperature control incubator for culturing mesenchymal stem cell balling, and comprises a bottle body for containing mesenchymal stem cells and a container for containing ultrasonic buffer water, the container is fixedly arranged on a horizontal bearing surface in the temperature control incubator, the bottle body is obliquely arranged, the bottom of the bottle body is positioned in the ultrasonic buffer water liquid of the container, the bottle body is driven to move by a driving device fixed on the horizontal bearing surface, and the driving device is configured to enable the bottle body to vibrate back and forth in the container along the horizontal direction and simultaneously drive the bottle body to move back and forth. And the bottle body also rotates around the axial direction of the bottle body. According to the utility model, the culture bottle simultaneously performs compound motion of horizontal oscillation and self rotation, so that the cell growth can be more uniformly stimulated, and the problem of poor balling effect caused by single rotation in the traditional method is solved. Through the movement mode, the cells are distributed more uniformly in the culture solution, and the formed spheres are more consistent in size and higher in quality.

Description

TECHNICAL FIELD

[0001] The utility model relates to mesenchymal stem cell balling technology field, concretely is adjustable angle's rotary jar type mesenchymal stem cell balling device. BACKGROUND

[0002] The mesenchymal stem cell three-dimensional balling culture technology is the key technology for constructing organoid model and realizing cell therapy industrialization, and the core lies in inducing stem cells to self-organize to form uniform functional spheroids through controllable mechanical microenvironment. The traditional culture method is to place the bottle containing mesenchymal stem cells into a container that can be heated in water bath, and an ultrasonic generator is arranged in the container. During the rotation of the bottle, the stem cells in the bottle are induced to form balls through ultrasonic waves and water bath heating. However, in the prior art, the rotation direction of the bottle is single, and the balling effect in the bottle is poor. UTILITY MODEL CONTENT

[0003] The utility model aims at providing adjustable angle's rotary jar type mesenchymal stem cell balling device to solve the problems raised in the above background technology.

[0004] To achieve the above object, the utility model provides the following technical scheme:

[0005] The adjustable angle's rotary jar type mesenchymal stem cell balling device is arranged in a temperature-controlled incubator to culture mesenchymal stem cell balls, comprising a bottle for containing mesenchymal stem cells and a container for containing ultrasonic buffer water. The container is fixed on a horizontal bearing surface in the temperature-controlled incubator. The bottle is inclinedly arranged, and the bottom of the bottle is located in the ultrasonic buffer water liquid in the container. The bottle is driven to move by a driving device fixed on the horizontal bearing surface. The driving device is configured to make the bottle reciprocate in the container along the horizontal direction while the bottle also rotates around its own axis.

[0006] Preferably, the driving device comprises a fixed frame fixed on the horizontal bearing surface and a stepping motor with adjustable inclination angle installed on the fixed frame. The driving shaft of the stepping motor is arranged obliquely upward, and a driving motor is fixedly connected to the driving shaft. A detachable bottle cap is arranged at the bottle mouth of the bottle. A driving shaft is fixed to the driving shaft of the driving motor, and the driving shaft is detachably connected to the bottle cap. The driving shaft of the driving motor is coaxially arranged with the bottle.

[0007] Preferably, the stepping motor is fixedly installed in a motor bracket, and the stepping motor is installed in the fixed frame through the motor bracket with adjustable inclination angle.

[0008] Preferably, opposite sides of the top of the motor support are fixed with rotating pins connected with the fixing frame in rotation, the bottom of the motor support is provided with an adjusting rod arranged horizontally through the motor support, the adjusting rod transversely penetrates the fixing frame, and the fixing frame is provided with an arc-shaped slot coaxially arranged with the rotating pin, the adjusting rod slides along the arc-shaped slot, so that the inclination angle of the stepping motor is adjusted, both ends of the adjusting rod are located outside the fixing frame, one end of the adjusting rod is fixed with a limiting disc, and the other end of the adjusting rod is threadedly connected with an adjusting knob, the adjusting knob is screwed to fix the adjusting rod, and thus the inclination angle of the stepping motor is fixed.

[0009] Preferably, the motor support comprises two fixed frames symmetrically arranged with respect to the stepping motor, the fixed frames enclose the stepping motor in a direction parallel to the driving shaft of the stepping motor, one end of the fixed frame towards the driving shaft of the stepping motor is fixed with a mounting plate, the mounting plate is fixedly connected with the stepping motor through bolts, the mounting plate is provided with a through hole for the driving shaft of the stepping motor to penetrate the mounting plate, and the bottom end of the fixed frame is fixed with a protrusion parallel to the driving shaft of the stepping motor, and the protrusions at the bottom ends of the two fixed frames are attached to each other, and the adjusting rod transversely penetrates between the two protrusions.

[0010] Compared with the prior art, the adjustable-angle rotating jar type mesenchymal stem cell sphere forming device has the following beneficial effects:

[0011] The adjustable-angle rotating jar type mesenchymal stem cell sphere forming device can more evenly stimulate cell growth by allowing the culture bottle to simultaneously perform horizontal oscillation and self-rotation, and solves the problem of poor sphere forming effect caused by single rotation in the traditional method. The motion mode makes the cells more evenly distributed in the culture solution, the formed spheres are more uniform in size, and the quality is higher. BRIEF DESCRIPTION OF DRAWINGS

[0012] Fig. 1 It is a structure schematic view of the adjustable-angle rotating jar type mesenchymal stem cell sphere forming device.

[0013] Fig. 2 It is a structure schematic view of the adjustable-angle rotating jar type mesenchymal stem cell sphere forming device.

[0014] Fig. 3 It is a structure schematic view of the adjustable-angle rotating jar type mesenchymal stem cell sphere forming device.

[0015] Fig. 4 It is a structure schematic view of the adjustable-angle rotating jar type mesenchymal stem cell sphere forming device.

[0016] Fig. 5 It is a structure schematic view of the adjustable-angle rotating jar type mesenchymal stem cell sphere forming device. DETAILED DESCRIPTION

[0017] Clearly, the described embodiments are merely a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0018] Please refer to Figs. 1-5 In the embodiments of the present application, the angle-adjustable rotating tank type mesenchymal stem cell ball formation device is used for culturing mesenchymal stem cell balls in a temperature-controlled incubator, and includes a bottle body 2 for containing mesenchymal stem cells and a container 1 for containing ultrasonic buffer water. The container 1 is fixed on a horizontal bearing surface in the temperature-controlled incubator, and the bottle body 2 is obliquely arranged with the bottom of the bottle body 2 located in the ultrasonic buffer water in the container 1. The bottle body 2 is driven to move by a driving device fixed on the horizontal bearing surface. The driving device is configured to make the bottle body 2 reciprocate in the container 1 in the horizontal direction while the bottle body 2 also rotates around its own axis.

[0019] The present application can more uniformly stimulate cell growth by allowing the culture bottle to simultaneously perform horizontal oscillation and self-rotation compound motion, and solves the problem of poor ball formation effect caused by single rotation in the traditional method. This motion mode allows the cells to be more uniformly distributed in the culture solution, and the formed balls are more uniform in size and higher in quality.

[0020] It should be noted that the container 1 is used for water bath heating of the bottle body 2, and a heating device and an ultrasonic generating device are arranged in the container 1. The temperature of the heating device and the frequency of the ultrasonic generating device can be adjusted, and they jointly act on the bottle body 2 to improve the ball formation effect. The technology of arranging the heating device and the ultrasonic generating device in the container 1 and the adjustment technology of the components are all mature technologies, and will not be described here.

[0021] In addition, in order to ensure the ball formation of mesenchymal stem cells, the airflow between the bottle body 2 and the outside world needs to be kept unobstructed, but the liquid in the bottle body 2 cannot flow out during rotation. Therefore, an opening is arranged on the top of the bottle body 2, and a diaphragm valve is fixed in the opening.

[0022] The driving device includes a fixed frame 3 fixed on the horizontal bearing surface and a step motor 4 installed on the fixed frame 3 and having an adjustable inclination angle. The driving shaft of the step motor 4 is arranged obliquely upward, and a driving motor 6 is fixedly connected to the driving shaft. A detachable bottle cap 21 is arranged at the bottle mouth of the bottle body 2. A driving shaft 7 is fixed to the driving shaft of the driving motor 6, and the driving shaft 7 is detachably connected to the bottle cap 21. The driving shaft of the driving motor 6 is coaxially arranged with the bottle body 2.

[0023] It should be noted that in actual application, the specifications of the bottle body 2 and the bottle cap 21 can be changed, and the inclination angle of the bottle body 2 is adjusted by adjusting the angle of the stepping motor 4. The model of the stepping motor 4 can be selected as 17HS19-2004S1, and the model of the driving motor 6 can be selected as N20 micro-reduction DC motor. The stepping motor 4 and the driving motor 6 are electrically connected to an external control terminal. In specific operation, the swinging amplitude, the swinging frequency of the horizontal reciprocating oscillation of the bottle body 2 driven by the stepping motor 4, and the rotating speed of the driving motor 6 can be controlled by the external control terminal. The control technology and power supply are both mature technologies, and will not be described here.

[0024] Specifically, the bottle cap 21 is fixed with a plug rod 22 coaxially arranged with the bottle cap 21. The end of the driving shaft 7 towards the bottle cap 21 is provided with a shaft hole for the plug rod 22 to be inserted into the driving shaft 7. The driving shaft 7 is threadedly connected with a clamping screw 71 for fixing the plug rod 22. The clamping screw 71 is arranged along the radial direction of the driving shaft 7. When the clamping screw 71 is tightened, the end of the clamping screw 71 inside the driving shaft 7 abuts against the side wall of the plug rod 22, or directly penetrates through the plug rod 22, thereby fixing the plug rod 22 in the shaft hole of the driving shaft 7. The other end of the driving shaft 7 is fixedly connected with the driving shaft of the driving motor 6.

[0025] The stepping motor 4 is fixedly installed in the motor bracket 5, and the stepping motor 4 is installed in the fixed frame 3 with an adjustable inclination angle through the motor bracket 5.

[0026] The opposite sides of the top of the motor bracket 5 are fixed with rotating pins 54 rotatably connected with the fixed frame 3. The bottom of the motor bracket 5 is provided with an adjusting rod 56 horizontally arranged through the motor bracket 5. The adjusting rod 56 transversely penetrates through the fixed frame 3, and the fixed frame 3 is provided with an arc-shaped slot 31 coaxially arranged with the rotating pin 54. The adjusting rod 56 slides along the arc-shaped slot 31, thereby adjusting the inclination angle of the stepping motor 4. The two ends of the adjusting rod 56 are located outside the fixed frame 3. One end of the adjusting rod 56 is fixed with a limiting disc 57, and the other end is threadedly connected with an adjusting knob 58. The adjusting rod 56 is fixed by tightening the adjusting knob 58, thereby fixing the inclination angle of the stepping motor 4.

[0027] When the adjusting knob 58 is tightened, the side wall of the adjusting knob 58 is attached to one side of the outer wall of the fixed frame 53, and the limiting disc 57 is pulled by the adjusting rod 56 to be attached to the other side of the outer wall of the fixed frame 53, thereby fixing the adjusting rod 56.

[0028] The motor support 5 comprises two fixed frames 53 symmetrically arranged relative to the stepping motor 4, the fixed frames 53 wrap the stepping motor 4 along a direction parallel to the driving shaft of the stepping motor 4, one end of the fixed frames 53 towards the driving shaft of the stepping motor 4 is fixed with a mounting plate 51, the mounting plate 51 is fixedly connected with the stepping motor 4 through bolts, the mounting plate 51 is provided with a through hole 52 for the driving shaft of the stepping motor 4 to pass through the mounting plate 51, the bottom end of the fixed frames 53 is fixed with a protrusion 55 parallel to the driving shaft of the stepping motor 4, the protrusions 55 at the bottom end of the two fixed frames 53 are mutually abutted, and an adjusting rod 56 transversely passes between the two protrusions 55; when the adjusting rod 56 moves along the arc-shaped slot 31, the stepping motor 4 is driven to rotate by the motor support 5, thereby realizing the angle adjustment of the stepping motor 4.

[0029] Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model, and the contents not described in detail in the specification all belong to the prior art known to those skilled in the art.

Claims

1. An angle-adjustable rotary canister type mesenchymal stem cell spheroidization device for culturing mesenchymal stem cell spheroids in a temperature-controlled incubator, comprising a bottle body (2) for containing mesenchymal stem cells and a container (1) for containing ultrasonic wave buffer water, the container (1) being fixed on a horizontal bearing surface in the temperature-controlled incubator, characterized in that, The bottle body (2) is obliquely arranged, and the bottom of the bottle body (2) is located in the ultrasonic buffer water liquid in the container (1); the bottle body (2) is driven to move by a driving device fixed on a horizontal bearing surface, and the driving device is configured to drive the bottle body (2) to reciprocate in the horizontal direction in the container (1) while the bottle body (2) rotates around its own axis.

2. The angle-adjustable, rotary, mesenchymal stem cell spheroidization device of claim 1, wherein, The driving device comprises a fixed frame (3) fixed on the horizontal bearing surface and a step motor (4) installed on the fixed frame (3) and adjustable in inclination angle; the driving shaft of the step motor (4) is obliquely upward arranged, and a driving motor (6) is fixedly connected to the driving shaft; a detachable bottle cap (21) is arranged at the bottle mouth of the bottle body (2); a driving shaft (7) is fixed to the driving shaft of the driving motor (6), and the driving shaft (7) is detachably connected to the bottle cap (21); and the driving shaft of the driving motor (6) is coaxially arranged with the bottle body (2).

3. The angle-adjustable, rotary, mesenchymal stem cell spheroidization device of claim 2, wherein, A plug rod (22) coaxially arranged with the bottle cap (21) is fixed to the bottle cap (21); an axial hole is formed in one end of the driving shaft (7) facing the bottle cap (21) for inserting the plug rod (22) into the driving shaft (7); a clamping screw (71) for fixing the plug rod (22) is threadedly connected to the driving shaft (7); the clamping screw (71) is arranged along the radial direction of the driving shaft (7); when the clamping screw (71) is tightened, one end of the clamping screw (71) located in the driving shaft (7) can abut against the side wall of the plug rod (22) or penetrate through the plug rod (22) to fix the plug rod (22) in the axial hole of the driving shaft (7); and the other end of the driving shaft (7) is fixedly connected with the driving shaft of the driving motor (6).

4. The angle-adjustable, rotary, mesenchymal stem cell spheroidization device of claim 2, wherein, The step motor (4) is fixedly installed in a motor bracket (5), and the step motor (4) is installed in the fixed frame (3) in an adjustable inclination angle through the motor bracket (5).

5. The angle-adjustable, rotary can-type mesenchymal stem cell spheroidization device according to claim 4, characterized by, Opposite sides of the top of the motor bracket (5) are fixed with rotating pins (54) rotationally connected with the fixed frame (3); the bottom of the motor bracket (5) is provided with an adjusting rod (56) horizontally arranged through the motor bracket (5); the adjusting rod (56) transversely penetrates through the fixed frame (3), and the fixed frame (3) is provided with an arc-shaped groove (31) coaxially arranged with the rotating pin (54); the adjusting rod (56) slides along the arc-shaped groove (31), so as to adjust the inclination angle of the step motor (4); the two ends of the adjusting rod (56) are located outside the fixed frame (3), one end of which is fixed with a limiting disc (57), and the other end is threadedly connected with an adjusting knob (58); the adjusting knob (58) is tightened to fix the adjusting rod (56), so as to fix the inclination angle of the step motor (4).

6. The angle-adjustable rotary jar mesenchymal stem cell spheroidization device according to claim 4 or 5, characterized in that, The motor support (5) comprises two fixed side frames (53) symmetrically arranged relative to the stepping motor (4), the fixed side frames (53) wrap the stepping motor (4) along a direction parallel to the driving shaft of the stepping motor (4), one end of the fixed side frames (53) towards the driving shaft of the stepping motor (4) is fixed with a mounting plate (51), the mounting plate (51) is fixedly connected with the stepping motor (4) through bolts, the mounting plate (51) is provided with a through hole (52) for the driving shaft of the stepping motor (4) to pass through the mounting plate (51), the bottom end of the fixed side frames (53) is fixed with a protrusion (55) parallel to the driving shaft of the stepping motor (4), the protrusions (55) at the bottom end of the two fixed side frames (53) are mutually abutted, and an adjusting rod (56) transversely passes between the two protrusions (55).

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