Microorganism sampling and culturing device for veterinary detection

Abstract

The invention provides a microorganism sampling and culturing device for veterinary detection, and relates to the technical field of microorganism sampling and culturing, the microorganism sampling and culturing device comprises a base, a plurality of diversion cabins and culturing grooves, the surface of the base is provided with the plurality of diversion cabins, the side edges of the diversion cabins are provided with the culturing grooves, and culturing mechanisms are placed in the culturing grooves; the culture mechanism is used for injecting a collected microorganism sample, the culture mechanism moves horizontally along the interior of the culture tank, the mixing assembly abuts against the body surface of an animal to be sampled, then the sampling assembly is controlled to be matched with the pushing mechanism, and then the sampling cotton can be pressed in a sampling area for sampling; in the whole process, the probability that the sample is possibly polluted by the external environment is reduced, the multiple culture dishes at the bottom can be controlled to move along the interior of the culture tank at the same time, and the switching process of several states of butt joint with a culture medium, culture sealing and oxygen conveying butt joint is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial sampling and culture, in particular to a microbial sampling and culture device for veterinary detection. BACKGROUND

[0002] The core purpose of microbial sampling and culture in veterinary detection is to accurately identify the pathogen causing animal diseases. By aseptically collecting samples from the infected parts of sick animals, including wounds, respiratory tract, digestive tract, etc., and culturing in specific culture medium in the laboratory, the trace amount of pathogen can be multiplied, thereby providing a basis for subsequent identification and drug sensitivity test. This process is of great significance: it not only can clearly diagnose the cause and avoid misjudgment, but more importantly, it can guide the veterinarian to choose the most effective antibacterial drugs like a "drug compass", achieve precise treatment and avoid drug abuse, and also plays an indispensable key role in monitoring epidemic diseases, evaluating the biological safety level of breeding farms and protecting public health, and is the core bridge connecting animal disease diagnosis and effective prevention and control.

[0003] In the prior art, the conventional veterinary microbial sampling and culture device has obvious deficiencies: the sampling process is usually open or step-by-step, the sampling cotton is exposed to the external environment after collection, which has a high risk of sample contamination; the subsequent sample elution, mixing with culture medium and dispensing, etc. rely on manual operation, which not only is tedious and inefficient, but also further increases the risk of contamination; at the same time, the whole culture process lacks integrated automatic control and closed environment protection, it is difficult to realize the immediate and closed culture of the sample after sampling, and it is also difficult to conveniently switch different gas environments during the culture process, which ultimately affects the accuracy and reliability of the detection. SUMMARY

[0004] The present application aims to at least solve one of the technical problems in the related art.

[0005] To this end, the present application provides a microbial sampling and culture device for veterinary detection, which can press the sampling cotton in the sampling area for sampling by pressing the mixing assembly against the surface of the animal to be sampled, then operating the sampling assembly and cooperating with the pushing mechanism, thereby reducing the probability of sample contamination by the external environment during the whole process, and can control the multiple culture dishes at the bottom to move along the inside of the culture groove, and realize the switching process of the docking, culture closure and oxygen delivery docking of the culture medium.

[0006] To achieve the above objectives, the present invention provides a microbial sampling and culture device for veterinary testing, comprising: a base, a flow guide chamber, and a culture tank. The surface of the base is provided with a plurality of flow guide chambers, and the side of the flow guide chamber is provided with a culture tank. A culture mechanism is placed inside the culture tank. The culture mechanism is used to inject the collected microbial sample and moves translationally along the inside of the culture tank. The culture device also includes a pushing mechanism, a mixing component, and a conveying pipe. The end of the flow guide chamber is connected to the conveying pipe, the top of the conveying pipe is connected to the mixing component, the end of the mixing component is connected to the docking pipe, and the end of the docking pipe is provided with a pushing mechanism. The pushing mechanism stores liquid culture medium inside and is used to convey the liquid culture medium inside to the inside of the mixing component. The mixing component is used for sampling and processing animal body fluids. The pushing mechanism delivers liquid culture medium into the mixing component and mixes it with the body fluid sample. The delivery pipe delivers the mixed sample from inside the mixing component to the inside of the culture device.

[0007] Furthermore, push notification providers include: The medium injection tube, a one-way valve, and a push rod are provided. The inside of the medium injection tube is used to store liquid medium. A push rod is inserted into the end of the medium injection tube. The end of the push rod is connected to a piston plate, which is embedded in the inside of the medium injection tube. A one-way valve is provided at the top of the medium injection tube.

[0008] Furthermore, the push mechanism also includes: An extension plate and a bonding plate are provided, wherein the extension plate is integrally formed on the side of the culture medium injection tube, and a bonding plate is integrally formed at the end of the extension plate. The bonding plate is used to press against the sampling area, and the one-way valve is used to add additional liquid culture medium toward the interior of the culture medium injection tube. The end of the culture medium injection tube is connected to a docking pipe, and the docking pipe is in communication with the interior of the culture medium injection tube. The push rod, in conjunction with the piston plate at the end, is used to push the liquid culture medium inside the culture medium injection tube toward the interior of the docking pipe.

[0009] Furthermore, the hybrid component includes: The mixture comprises a mixed interlayer, a moving channel, a pressing sleeve, and an adhesive plate. The moving channel is integrally formed in the middle of the mixed interlayer, and a pressing sleeve is integrally formed at the end of the moving channel. An adhesive plate is attached to the end of the pressing sleeve. The adhesive plate is used to seal the inside of the pressing sleeve, and the pressing sleeve is used to press against the area to be sampled.

[0010] Furthermore, the hybrid component also includes: A sampling assembly, comprising: a lead screw, a knob, a top spring, a connecting column, and sampling cotton; The end of the lead screw is integrally formed with a knob, and a top spring is welded to the end of the lead screw. A connecting post is installed at the end of the top spring. Sampling cotton is attached to the side and end of the connecting post. The connecting post and the sampling cotton are both cylindrical in shape. The lead screw and the top spring are both located in the internal area of ​​the moving channel. The top spring is used to press the connecting post and the sampling cotton into the sampling area.

[0011] Furthermore, the mixing assembly also includes a partition, a plug-in hole, and a threaded sleeve. The lead screw passes through the inside of the threaded sleeve, and the lead screw, in conjunction with the threaded sleeve, drives the end spring, connecting post, and sampling cotton to rotate and translate. The lead screw passes through the inside of the plug-in hole. The edge of the partition is integrally formed with the inner wall of the moving channel. The partition is used to block and squeeze the end of the sampling cotton. After the partition squeezes the end of the sampling cotton, it is used to discharge the body fluid sample adsorbed in the sampling cotton. The interior of the mixing interlayer is in communication with the interior of the delivery pipe and the interior of the docking pipe. The adhesive plate is fixed to the outside of the pressing sleeve by adhesive. The adhesive plate is also used to block the sampling cotton. The diameter of the connecting column is the same as the diameter of the insertion hole.

[0012] Furthermore, the front end of the culture tank is provided with a sliding groove, the side of the flow guide chamber is provided with a groove, both ends of the inner wall of the groove are inserted with spring rods, the end of each spring rod is welded with a sealing block, and the inner wall of the groove is provided with a sample injection port.

[0013] Furthermore, a diversion pipe is provided at one end of the culture tank, and the interior of the diversion pipe is connected to the interior of each flow guide chamber. The conveying pipe is used to convey the sample into the interior of the flow guide chamber, and the flow guide chamber is connected to the interior of the culture mechanism through the sample injection port. The inner wall of the groove is also provided with a vent. The other end of the culture tank is provided with an end plate. The side of the end plate is provided with a vent pipe. The vent pipe is connected to the vent through the interior of the flow guide chamber. Each groove is provided with two sealing blocks. The sealing blocks are used to seal the sample injection port and the vent.

[0014] Furthermore, the cultivation facility includes: The petri dish, observation cover, and connector are provided. The observation cover is installed on the top of the petri dish, and the top of the observation cover is connected to the connector. The end of the connector is used to be embedded in the interior of a groove, and the diameter of the connector is the same as the height of the groove. The connector is used to communicate with the sample injection port or the vent.

[0015] Furthermore, the cultivation facility also includes: The system comprises a base plate, a sliding plate, and a pull rod. The base plate is disposed at the bottom of each culture dish. A gap is provided at the bottom of the flow chamber, through which the base plate passes. Each culture dish is integrally connected by the base plate. The front end of the base plate protrudes from the inside of the groove. The front end of the base plate is integrally formed with a sliding plate, and a pull rod is installed on the surface of the sliding plate.

[0016] The technical solution provided by this invention may include the following beneficial effects: 1. When using this veterinary testing microbial sampling and culture device, the mixing component at the top is placed directly against the surface of the animal to be sampled. Then, by manipulating the sampling component and cooperating with the pushing mechanism, the sampling cotton is pressed into the sampling area for sampling. The sample is then mixed with the flowing liquid culture medium and automatically transported to the culture mechanism at the end. Throughout the process, the sampling cotton is always in a closed environment, reducing the probability of contamination of the sample by the external environment.

[0017] 2. The veterinary testing microbial sampling and culture device has a culture mechanism at the bottom that can simultaneously control multiple culture dishes at the bottom to move along the inside of the culture tank and realize the switching process of several states such as docking with the culture medium, culture sealing, and oxygen supply docking. The operation process is simple and convenient, and with the sealing block, it can reduce the contamination of the internal environment of the culture medium by the external environment during the switching process, thereby improving the effectiveness of the final culture.

[0018] 3. The veterinary testing microbial sampling and culture device is located inside the mixing component. When collecting animal body fluids using the sampling component, after the sampling cotton is mixed with the liquid culture medium, the sampling cotton can be directly squeezed by the movement of the sampling component. This process can increase the amount of sample collected. This process also does not require the sampling cotton to be exposed to the outside, thus increasing the total amount of sample collected in a single sampling.

[0019] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0020] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the following description of the embodiments taken in conjunction with the accompanying drawings, wherein: Figure 1 This is a schematic diagram of the structure of a veterinary microbial sampling and culture device according to an embodiment of the present invention; Figure 2This is a schematic diagram showing the connection between the pushing mechanism and the mixing component in a veterinary microbial sampling and culture device according to an embodiment of the present invention; Figure 3 This is a cross-sectional view of the mixing component in a veterinary microbial sampling and culture device according to an embodiment of the present invention; Figure 4 This is a schematic diagram of the sampling component structure in a veterinary microbial sampling and culture device according to an embodiment of the present invention; Figure 5 This is a schematic diagram of the base structure in a veterinary microbial sampling and culture device according to an embodiment of the present invention; Figure 6 This is a schematic diagram of the culture mechanism structure in a veterinary microbial sampling and culture device according to an embodiment of the present invention; Figure 7 This is a schematic diagram of the flow guide chamber in a veterinary microbial sampling and culture device according to an embodiment of the present invention; As shown in the figure: 1. Base; 2. Culture tank; 3. Culture mechanism; 4. Delivery pipe; 5. Pushing mechanism; 6. Mixing assembly; 7. Culture medium injection pipe; 8. One-way valve; 9. Push rod; 10. Extension plate; 11. Adhesive plate; 12. Connecting pipe; 13. Mixing jacket; 14. Moving channel; 15. Sampling assembly; 16. Pressing sleeve; 17. Adhesive plate; 18. Partition; 19. Insertion hole; 20. Threaded sleeve; 21. 21. Lead screw; 22. Knob; 23. Top spring; 24. Connecting column; 25. Sampling cotton; 26. Diversion pipe; 27. Flow guide chamber; 28. Gap; 29. ​​Slide groove; 30. End plate; 31. Ventilation pipe; 32. Base plate; 33. Petri dish; 34. Observation cover; 35. Connecting interface; 36. Sliding plate; 37. Pull rod; 38. Groove; 39. Sample injection port; 40. Vent; 41. Sealing block; 42. Spring rod. Detailed Implementation

[0021] Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the invention, and should not be construed as limiting the invention. Rather, embodiments of the invention include all variations, modifications, and equivalents falling within the spirit and scope of the appended claims.

[0022] like Figures 1 to 7As shown in the figure, an embodiment of the present invention proposes a microbial sampling and culture device for veterinary testing, comprising: a base 1, a flow guiding chamber 27 and a culture tank 2. The surface of the base 1 is provided with a plurality of flow guiding chambers 27, and the side of the flow guiding chamber 27 is provided with a culture tank 2. A culture mechanism 3 is placed inside the culture tank 2. The culture mechanism 3 is used to inject the collected microbial sample, and the culture mechanism 3 moves translationally along the inside of the culture tank 2. The culture device also includes a pushing mechanism 5, a mixing component 6, and a conveying pipe 4. The end of the flow guide chamber 27 is connected to the conveying pipe 4, the top of the conveying pipe 4 is connected to the mixing component 6, the end of the mixing component 6 is connected to the docking pipe 12, and the end of the docking pipe 12 is provided with a pushing mechanism 5. The pushing mechanism 5 stores liquid culture medium inside and is used to convey the liquid culture medium inside to the inside of the mixing component 6. The mixing component 6 is used to sample and process animal body fluids. The pushing mechanism 5 delivers the liquid culture medium into the mixing component 6 and mixes it with the body fluid sample. The delivery pipe 4 delivers the mixed sample inside the mixing component 6 into the culture unit 3.

[0023] Specifically, when using this veterinary testing microbial sampling and culture device, liquid culture medium is injected into the push mechanism 5 through the one-way valve 8 at the top of the push mechanism 5. Then, the mixing component 6 is manually pressed onto the sampling area on the animal's body surface. By rotating the knob 22 in the mixing component 6, the sampling cotton 25 on the inner side can be controlled to perform adhesion sampling on the sampling area on the animal's body surface. Then, the push mechanism 5 is controlled to transport the culture medium into the mixing component 6, which washes and mixes the surface of the sampling cotton 25. Finally, the culture medium liquid mixed with the sample is transported along the delivery pipe 4 to the culture unit 3 for static culture. During the culture process, by controlling the culture unit 3 to move horizontally inside the culture tank 2, the process of replenishing the culture medium nutrient solution, sealing, or changing the air environment can be achieved.

[0024] In this embodiment, the pushing mechanism 5 includes: The medium injection tube 7, the one-way valve 8, and the push rod 9 are provided. The inside of the medium injection tube 7 is used to store liquid medium. The push rod 9 is inserted into the end of the medium injection tube 7. The end of the push rod 9 is connected to a piston plate, and the piston plate is embedded in the inside of the medium injection tube 7. The one-way valve 8 is provided at the top of the medium injection tube 7.

[0025] The push mechanism 5 also includes: The extension plate 10 and the bonding plate 11 are integrally formed on the side of the culture medium injection tube 7, and the bonding plate 11 is integrally formed at the end of the extension plate 10. The bonding plate 11 is used to press on the sampling area, and the one-way valve 8 is used to add additional liquid culture medium towards the inside of the culture medium injection tube 7. The end of the culture medium injection tube 7 is connected to the docking pipe 12, and the docking pipe 12 is in communication with the interior of the culture medium injection tube 7. The push rod 9, in conjunction with the piston plate at the end, is used to push the liquid culture medium inside the culture medium injection tube 7 toward the interior of the docking pipe 12.

[0026] Specifically, by connecting the external liquid culture medium pipeline to the one-way valve 8, the liquid culture medium can be delivered into the culture medium injection tube 7. After stopping the injection, the culture medium inside the culture medium injection tube 7 can be pushed towards the mixing component 6 by pushing the push rod 9. During sampling, the rear bonding plate 11 is pressed onto the sample area of ​​the animal body surface to be collected. The extension plate 10 area is held by the fingers, and the back of the hand is used to press against one side of the bonding plate 11. The pushing process of the push rod 9 can be completed with one hand, so that the other hand can directly control the mixing component 6 and drive the sampling component 15 to move.

[0027] In this embodiment, the hybrid component 6 includes: The mixture includes a hybrid interlayer 13, a moving channel 14, a pressing sleeve 16, and an adhesive plate 17. The moving channel 14 is integrally formed in the middle of the hybrid interlayer 13, and the pressing sleeve 16 is integrally formed at the end of the moving channel 14. The adhesive plate 17 is attached to the end of the pressing sleeve 16. The adhesive plate 17 is used to seal the inside of the pressing sleeve 16, and the pressing sleeve 16 is used to press against the area to be sampled.

[0028] The hybrid component 6 also includes: Sampling component 15, which includes: lead screw 21, knob 22, top spring 23, connecting post 24 and sampling cotton 25; A knob 22 is integrally formed at the end of the lead screw 21. A top spring 23 is welded to the end of the lead screw 21. A connecting post 24 is installed at the end of the top spring 23. Sampling cotton 25 is attached to the side and end of the connecting post 24. The connecting post 24 and the sampling cotton 25 are both cylindrical in shape. The lead screw 21 and the top spring 23 are both located in the internal area of ​​the moving channel 14. The top spring 23 is used to press the connecting post 24 and the sampling cotton 25 into the sampling area.

[0029] The mixing component 6 also includes a partition plate 18, a plug hole 19 and a threaded sleeve 20. The lead screw 21 passes through the inside of the threaded sleeve 20, and the lead screw 21, in conjunction with the threaded sleeve 20, drives the end spring 23, the connecting post 24 and the sampling cotton 25 to rotate and translate. The lead screw 21 passes through the inside of the plug hole 19. Inside the mixing component 6, when animal body fluids are collected using the sampling component 15, after the sampling cotton 25 is mixed with the liquid culture medium, the sampling component 15 continues to move, which can directly squeeze the sampling cotton 25. This process can increase the amount of sample collected, and it does not require the sampling cotton 25 to be exposed to the outside, thus increasing the total amount of sample collected in a single sampling.

[0030] The edge of the partition 18 is integrally formed with the inner wall of the moving channel 14. The partition 18 is used to block and squeeze the end of the sampling cotton 25. After the partition 18 squeezes the end of the sampling cotton 25, it is used to discharge the body fluid sample adsorbed in the sampling cotton 25. The interior of the mixing interlayer 13 is in communication with the interior of the conveying pipe 4 and the interior of the docking pipe 12. The adhesive plate 17 is fixed to the outside of the pressing sleeve 16 by adhesive. The adhesive plate 17 is also used to block the sampling cotton 25. The diameter of the connecting post 24 is the same as the diameter of the insertion hole 19.

[0031] In use, simply place the top mixing component 6 against the surface of the animal to be sampled, then operate the sampling component 15 and push the pushing mechanism 5 to press the sampling cotton 25 onto the sampling area for sampling. The sample is then mixed with the flowing liquid culture medium and automatically transported to the end culture mechanism 3. Throughout the process, the sampling cotton 25 is always in a closed environment, reducing the probability of contamination of the sample by the external environment.

[0032] Specifically, by rotating the control knob 22, the lead screw 21 can be driven to rotate synchronously. The lead screw 21, in conjunction with the threaded sleeve 20, can move outward, thereby pulling the end spring 23 and the connecting post 24 to move outward. During sampling, the adhesive plate 17 at the end of the pressing sleeve 16 needs to be removed from the end of the pressing sleeve 16. At this time, the pressing sleeve 16 can be directly pressed against the area to be sampled. In this process, the blocking of the sampling cotton 25 by the adhesive plate 17 can be changed to the blocking of the sampling cotton 25 by the sampling area.

[0033] As the screw 21 moves outward after the knob 22 is turned, the top spring 23, initially in a compressed state, continues to push the connecting post 24 and the sampling cotton 25, ensuring that the sampling cotton 25 remains in contact with the sampling area. With the rotation of the screw 21, the top spring 23, connecting post 24, and sampling cotton 25 also rotate, allowing the sampling cotton 25 to fully contact the body fluid. Once the top spring 23 is completely relaxed, the screw 21 pulls the top spring 23 outward with the subsequent rotation of the knob 22, ultimately pulling the sampling cotton 25 into the middle area of ​​the moving channel 14. Finally, the connecting post 24 passes through the insertion hole 19. At this point, the sampling cotton 25 is blocked by the partition 18 and compressed, squeezing out the body fluid adsorbed in the sampling cotton 25. During this process, it mixes with the injected culture medium and is transported along the delivery pipe 4 towards the culture mechanism 3.

[0034] In this embodiment, the front end of the culture tank 2 is provided with a sliding groove 29, the side of the flow guide chamber 27 is provided with a groove 38, both ends of the inner wall of the groove 38 are inserted with spring rods 42, and the end of each spring rod 42 is welded with a sealing block 41. The inner wall of the groove 38 is provided with a sample injection port 39.

[0035] One end of the culture tank 2 is provided with a diversion pipe 26, the interior of the diversion pipe 26 is connected to the interior of each flow guide chamber 27, the conveying pipe 4 is used to convey the sample into the interior of the flow guide chamber 27, and the flow guide chamber 27 is connected to the interior of the culture mechanism 3 through the sample injection port 39. The inner wall of the groove 38 is also provided with a vent 40. The other end of the culture tank 2 is provided with an end plate 30. The side of the end plate 30 is provided with a vent pipe 31. The vent pipe 31 is connected to the vent 40 through the interior of the flow guide chamber 27. Each groove 38 is provided with two sealing blocks 41. The sealing blocks 41 are used to seal the sample injection port 39 and the vent 40.

[0036] Specifically, the sample doped with culture medium enters the interior of the diversion pipe 26 through the delivery pipe 4, and then enters the guide chamber 27 through the diversion pipe 26. Finally, it flows into the culture mechanism 3 from the sample injection port 39 of the side groove 38 of the guide chamber 27. The bottom of the culture mechanism 3 passes through the interior of the gap 28, and with the help of the groove 38 and the slide 29, the culture mechanism 3 can slide directly along the culture tank 2.

[0037] During the sliding process, the culture mechanism 3 will move along the inside of the groove 38 and change the docking state between the culture mechanism 3 and the sample injection port 39 or the vent 40 inside the groove 38. When the culture mechanism 3 docks with the sample injection port 39, the culture medium and sample can be injected into the culture mechanism 3 through the pushing mechanism 5. At this time, the vent 40 is blocked by the nearby sealing block 41. When the culture mechanism 3 moves to the other end and finally docks with the vent 40, the nutrient solution or oxygen that needs to be replenished can be delivered to the culture mechanism 3 through the ventilation pipe 31. At this time, the sample injection port 39 is blocked by the nearby sealing block 41.

[0038] In this embodiment, the culture mechanism 3 includes: The petri dish 33, the observation cover 34, and the interface 35 are provided. The observation cover 34 is installed on the top of the petri dish 33. The top of the observation cover 34 is connected to the interface 35. The end of the interface 35 is used to be embedded in the interior of the groove 38. The diameter of the interface 35 is the same as the height of the groove 38. The interface 35 is used to communicate with the sample injection port 39 or the vent 40.

[0039] The cultivation facility 3 also includes: The base plate 32, the sliding plate 36, and the pull rod 37 are provided. The base plate 32 is disposed at the bottom of each culture dish 33. The bottom of the flow guide chamber 27 is provided with a gap 28. The base plate 32 passes through the inside of the gap 28. Each culture dish 33 is integrally connected by the base plate 32. The front end of the base plate 32 extends out from the inside of the slide groove 29. The front end of the base plate 32 is integrally formed with the sliding plate 36. The pull rod 37 is installed on the surface of the sliding plate 36.

[0040] In the bottom culture mechanism 3, multiple culture dishes 33 at the bottom can be controlled to move along the inside of the culture tank 2 at the same time, and the switching process of several states such as docking with the culture medium, culture sealing and oxygen delivery docking can be realized. The operation process is simple and convenient, and with the sealing block 41, the pollution caused by the external environment to the internal environment of the culture medium can be reduced during the switching process, thus improving the effectiveness of the final culture.

[0041] Specifically, by directly pulling the front lever 37, the bottom plate 32 can be moved horizontally by the sliding plate 36, ultimately moving each culture dish 33 along the culture tank 2. During this process, the top interface 35 can be connected to the sample injection port 39 or the vent 40 respectively. In the same process, the interface 35 can also push the sealing blocks 41 at both ends so that when the interface 35 is removed from the sample injection port 39 or the vent 40, the spring rod 42 can cooperate with the sealing block 41 to seal the sample injection port 39 or the vent 40.

[0042] The terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance. Furthermore, in the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0043] Any process or method description in the flowchart or otherwise herein can be understood as representing a module, segment, or portion of code comprising one or more executable instructions for implementing a particular logical function or process, and the scope of the preferred embodiments of the invention includes additional implementations in which functions may be performed not in the order shown or discussed, including substantially simultaneously or in reverse order depending on the functions involved, as will be understood by those skilled in the art to which embodiments of the invention pertain.

[0044] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0045] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A microbial sampling and culture device for veterinary testing, characterized in that, include: The base (1), the flow guide chamber (27) and the culture tank (2) are provided. The surface of the base (1) is provided with a plurality of flow guide chambers (27). The side of the flow guide chamber (27) is provided with a culture tank (2). The culture tank (2) is placed inside the culture tank (2). The culture tank (3) is used to inject the collected microbial samples. The culture tank (3) moves along the inside of the culture tank (2). The culture device also includes a pushing mechanism (5), a mixing component (6), and a conveying pipe (4). The end of the flow guide chamber (27) is connected to the conveying pipe (4), the top of the conveying pipe (4) is connected to the mixing component (6), the end of the mixing component (6) is connected to the docking pipe (12), and the end of the docking pipe (12) is provided with a pushing mechanism (5). The pushing mechanism (5) stores liquid culture medium inside, and the pushing mechanism (5) is used to convey the liquid culture medium inside to the inside of the mixing component (6). The mixing component (6) is used to sample and process animal body fluids. The pushing mechanism (5) delivers the liquid culture medium into the mixing component (6) and mixes it with the body fluid sample. The delivery pipe (4) delivers the mixed sample inside the mixing component (6) into the culture mechanism (3).

2. The veterinary microbial sampling and culture device according to claim 1, characterized in that, The push mechanism (5) includes: The medium injection tube (7), the one-way valve (8), and the push rod (9) are provided. The inside of the medium injection tube (7) is used to store liquid medium. The end of the medium injection tube (7) is fitted with a push rod (9). The end of the push rod (9) is connected to a piston plate, and the piston plate is embedded in the inside of the medium injection tube (7). The one-way valve (8) is provided at the top of the medium injection tube (7).

3. The veterinary microbial sampling and culture device according to claim 2, characterized in that, The push mechanism (5) also includes: The extension plate (10) and the bonding plate (11) are integrally formed on the side of the culture medium injection tube (7), and the bonding plate (11) is integrally formed at the end of the extension plate (10). The bonding plate (11) is used to press on the sampling area, and the one-way valve (8) is used to add additional liquid culture medium toward the inside of the culture medium injection tube (7). The end of the culture medium injection tube (7) is connected to a docking pipe (12), and the docking pipe (12) is in communication with the interior of the culture medium injection tube (7). The push rod (9) works with the piston plate at the end to push the liquid culture medium inside the culture medium injection tube (7) toward the interior of the docking pipe (12).

4. The veterinary microbial sampling and culture device according to claim 2, characterized in that, The hybrid component (6) includes: The mixed interlayer (13), the moving channel (14), the pressing sleeve (16) and the adhesive plate (17) are integrated in the middle of the mixed interlayer (13), the pressing sleeve (16) is integrated at the end of the moving channel (14), and the adhesive plate (17) is attached to the end of the pressing sleeve (16). The adhesive plate (17) is used to seal the inside of the pressing sleeve (16), and the pressing sleeve (16) is used to press against the sampling area.

5. The veterinary microbial sampling and culture device according to claim 4, characterized in that, The hybrid component (6) also includes: The sampling assembly (15) includes: a lead screw (21), a knob (22), a top spring (23), a connecting column (24), and a sampling cotton (25); The end of the lead screw (21) is integrally formed with a knob (22), and the end of the lead screw (21) is welded with a top spring (23). The end of the top spring (23) is equipped with a connecting post (24). The side and end of the connecting post (24) are covered with sampling cotton (25). The connecting post (24) and the sampling cotton (25) are both cylindrical in shape. The lead screw (21) and the top spring (23) are both located in the internal area of ​​the moving channel (14). The top spring (23) is used to press the connecting post (24) and the sampling cotton (25) into the sampling area.

6. The veterinary microbial sampling and culture device according to claim 5, characterized in that, The mixing component (6) also includes a partition (18), a plug hole (19) and a threaded sleeve (20). The lead screw (21) passes through the inside of the threaded sleeve (20), and the lead screw (21) works with the threaded sleeve (20) to drive the end spring (23), the connecting post (24) and the sampling cotton (25) to rotate and translate. The lead screw (21) passes through the inside of the plug hole (19). The edge of the partition (18) is integrally formed with the inner wall of the moving channel (14). The partition (18) is used to block and squeeze the end of the sampling cotton (25). After the partition (18) squeezes the end of the sampling cotton (25), it is used to discharge the body fluid sample adsorbed in the sampling cotton (25). The interior of the mixing interlayer (13) is connected to the interior of the conveying pipe (4) and the interior of the docking pipe (12). After the adhesive plate (17) is fixed to the outside of the pressing sleeve (16) by adhesive, the adhesive plate (17) is also used to block the sampling cotton (25). The diameter of the connecting column (24) is the same as the diameter of the insertion hole (19).

7. The veterinary microbial sampling and culture device according to claim 4, characterized in that, The culture tank (2) has a sliding groove (29) at the front end, and the flow guide chamber (27) has a groove (38) on the side. Spring rods (42) are inserted into both ends of the inner wall of the groove (38), and a sealing block (41) is welded to the end of each spring rod (42). A sample injection port (39) is provided on the inner wall of the groove (38).

8. The veterinary microbial sampling and culture device according to claim 7, characterized in that, One end of the culture tank (2) is provided with a diversion pipe (26), the interior of the diversion pipe (26) is connected to the interior of each flow guide chamber (27), the conveying pipe (4) is used to convey the sample to the interior of the flow guide chamber (27), and the flow guide chamber (27) is connected to the interior of the culture mechanism (3) through the sample injection port (39); The inner wall of the groove (38) is also provided with a vent (40). The other end of the culture tank (2) is provided with an end plate (30). The side of the end plate (30) is provided with a vent pipe (31). The vent pipe (31) is connected to the vent (40) through the interior of the flow guide chamber (27). Each groove (38) is provided with two sealing blocks (41), and the sealing blocks (41) are used to seal the sample injection port (39) and the vent (40).

9. The veterinary microbial sampling and culture device according to claim 7, characterized in that, The cultivation facility (3) includes: The petri dish (33), observation cover (34) and interface (35) are provided. The observation cover (34) is installed on the top of the petri dish (33). The top of the observation cover (34) is connected to the interface (35). The end of the interface (35) is used to be embedded in the interior of the groove (38). The diameter of the interface (35) is the same as the height of the groove (38). The interface (35) is used to communicate with the sample injection port (39) or the vent (40) respectively.

10. The veterinary microbial sampling and culture device according to claim 9, characterized in that, The culture facility (3) also includes: The base plate (32), the sliding plate (36), and the pull rod (37) are provided. The base plate (32) is set at the bottom of each petri dish (33). The bottom of the flow guide chamber (27) is provided with a gap (28). The base plate (32) passes through the inside of the gap (28). Each petri dish (33) is integrally connected through the base plate (32). The front end of the base plate (32) passes through the inside of the slide groove (29). The front end of the base plate (32) is integrally formed with the sliding plate (36). The surface of the sliding plate (36) is equipped with the pull rod (37).

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