Selenium-rich bacterial fertilizer bacterial strain cultivation device and cultivation method thereof

Abstract

The application belongs to the technical field of strain cultivation, and particularly relates to a selenium-rich bacterial fertilizer strain cultivation device and a cultivation method thereof. The device comprises a cultivation box, a plurality of object placing plates are arranged in the cultivation box, and strains are placed on the object placing plates. A uniform control gas production device is arranged on the cultivation box. The uniform control gas production device comprises gas production square boxes which are installed on the top of the cultivation box. Two gas production square boxes are symmetrically arranged and oppositely opened. A rotating disc is installed on the outer wall of the cultivation box. A double-action stirring assembly is arranged on the rotating disc. The double-action stirring assembly comprises rotating columns which are installed on the edge of the side of the rotating disc away from the cultivation box. A driving gear is installed on the top of the cultivation box. The strains in different levels in the cultivation box can be targetedly operated, and dead angles in ventilation are avoided. The regional waste gas can be smoothly discharged, and oxygen supply is sufficient. The ventilation limitation is reduced, the selenium conversion efficiency is improved, the strains can be uniformly enriched in selenium, and finally the cultivation effect of the device is improved.

Description

Technical Field

[0001] This invention belongs to the field of microbial strain cultivation technology, specifically a device and method for cultivating selenium-enriched microbial fertilizer strains. Background Technology

[0002] With the surge in demand for selenium-enriched agricultural products, the market demand for selenium-enriched microbial fertilizers has also increased. The core quality of selenium-enriched microbial fertilizers depends on the activity, proliferation, and selenium conversion efficiency of the selenium-enriched microbial strains. Therefore, strain cultivation is a key process in its production. Existing microbial strain cultivation devices for selenium-enriched fertilizers require the introduction of sterile air into the box containing the microbial strains to ensure oxygen supply and expel metabolic waste gases, thus avoiding inhibition of microbial growth. However, in actual use, the device cannot target different levels of microbial strains within the box, easily creating ventilation dead zones. This leads to difficulty in expelling waste gases and insufficient oxygen supply in these areas, not only increasing ventilation limitations but also affecting selenium conversion efficiency. This prevents the microbial strains from being uniformly enriched with selenium, ultimately reducing the cultivation effect of the device. Summary of the Invention

[0003] In view of the above situation and to overcome the defects of the prior art, the present invention provides a selenium-enriched microbial fertilizer strain cultivation device and cultivation method, which effectively solves the problems in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a selenium-enriched microbial fertilizer inoculum cultivation device, comprising an incubation box; the incubation box is provided with several placement plates on which the inoculum is placed; the incubation box is equipped with a gas-generating device for targeted operation on inoculum at different levels; the gas-generating device includes a gas-generating box installed on the top of the incubation box; two gas-generating boxes are symmetrically arranged with their openings facing each other; a rotating disc is installed on the outer wall of the incubation box; a re-moving stirring component is provided on the rotating disc for dispersing the inoculum; the re-moving stirring component includes a rotating column installed on the edge of the rotating disc away from the incubation box; a drive gear is installed on the top of the incubation box; an angle-adjusting rotation unit is provided on the drive gear for increasing the ventilation range; the angle-adjusting rotation unit includes a drive base installed inside the incubation box; a drive shaft is installed on the drive base; the drive shaft is rotatably connected to the drive gear.

[0005] Preferably, a drive seat is mounted on the drive shaft; the number of drive seats is the same as the shelf, and each drive seat is located above the shelf; a hollow circular block is rotatably connected inside the drive seat; an air jet is mounted on the outer wall of the hollow circular block, and the two are connected.

[0006] Preferably, a first bevel gear is mounted on the side of the drive seat and is rotatably connected to the hollow circular block; a first base is mounted on the drive seat; an angle-adjusting gear is mounted on the top of the first base; a second bevel gear is mounted on the bottom of the first base; the second bevel gear and the angle-adjusting gear are rotatably connected; the second bevel gear and the first bevel gear are meshed; a half-cut toothed ring is installed inside the incubation box; the half-cut toothed ring is meshed with the angle-adjusting gear; the center of the half-cut toothed ring and the drive shaft are coaxial.

[0007] Preferably, the incubator has a transfer tube installed on its side wall; two transfer tubes are symmetrically arranged; a first bend tube is installed on the top of each of the two gas generating boxes; the ends of the two first bend tubes away from the gas generating boxes are respectively connected to the two transfer tubes; the first bend tubes and the gas generating boxes are connected to the transfer tubes; an L-shaped hose is installed on the side wall of the transfer tube; the number and position of the L-shaped hoses correspond to the drive seat; one end of the L-shaped hose is connected to the transfer tube, and the other end passes through the side wall of the drive seat and is located inside the hollow block; the hollow block and the L-shaped hose are connected.

[0008] Preferably, the two gas generating boxes are equipped with air outlet nozzles and air inlet pipes on opposite sides; the air outlet nozzles and air inlet pipes correspond one-to-one with the two gas generating boxes; one gas generating box is equipped with valve group A, and the other gas generating box is equipped with valve group B; valve group A includes a first valve port and a second valve port; the first valve port is connected to the air outlet nozzle, and the second valve port is connected to one of the first bend pipes; valve group B includes a third valve port and a fourth valve port; the third valve port is connected to the air inlet pipe, and the fourth valve port is connected to the other first bend pipe; a sterile air filter is installed on the incubator; the end of the air inlet pipe away from the gas generating box is connected to the sterile air filter.

[0009] Preferably, a third bevel gear is installed on the side of the drive base away from the drive shaft; the third bevel gear is rotatably connected to the drive shaft; a fourth bevel gear is installed inside the incubation box; the fourth bevel gear is rotatably connected to the rotating disk; the fourth bevel gear meshes with the third bevel gear; a second base is installed on the outer wall of the incubation box; a through positioning cylinder is provided on the side of the second base near the rotating column; the positioning cylinder and the second base are slidably fitted; a rectangular horizontal block is installed on the positioning cylinder; the rectangular horizontal block is located on the side of the rotating disk away from the incubation box; a through rectangular groove is provided on the side of the rectangular horizontal block near the rotating disk; the rotating column is located in the rectangular groove, and the two are slidably fitted; a positioning rack is provided on the side of the rectangular horizontal block away from the positioning cylinder.

[0010] Preferably, a positioning gear is installed on the side wall of the incubator; the positioning gear is meshed with a positioning rack; a driving gear is installed on the positioning gear, and the two are rotatably connected; the driving gear is installed on the inner wall of the incubator; driven gears are meshed on both sides of the driving gear; the driven gears are installed on the inner wall of the incubator; irrigation pipes are connected to both the driven gear and the driving gear; the irrigation pipes are located inside the shelf; and a stirring rod is installed on the outer wall of the irrigation pipes.

[0011] Preferably, the top of the incubator is provided with two symmetrical third bases; the two third bases are located between the openings of the two gas generating boxes; a through guide cylinder is provided on the side of the third base near the gas generating box; the guide cylinder and the third base are slidably engaged; one end of the guide cylinder is connected to a guide block, and the other end is connected to a gas generating plate; the gas generating plate is fitted into the gas generating box, and the two are slidably engaged.

[0012] Preferably, a drive motor is installed on the top of the incubator; a drive cam is installed at the output end of the drive motor; the guide block is located on the rotation path of the drive cam sidewall on the side away from the gas-generating plate; two guide blocks are located on both sides of the drive cam and are symmetrically arranged with the center of the drive cam as the axis of symmetry; a guide spring is sleeved on the guide cylinder; one end of the guide spring is connected to the third base and the other end is connected to the guide block; a rectangular gear frame is installed on the guide block; the drive gear is located inside the rectangular gear frame and the two are meshed together.

[0013] This invention also provides a method for cultivating selenium-enriched microbial fertilizer strains, comprising the following steps:

[0014] S1. Place the inoculum to be cultivated on the shelf to ensure that the inoculum is evenly enriched with selenium in the incubator.

[0015] S2. Operate the gas-generating device to perform targeted operations on bacteria at different levels and heights;

[0016] S3. The bacteria are dispersed by the compound stirring component to avoid the waste gas being unable to be discharged due to the accumulation and compression of bacteria;

[0017] S4. Control the angle adjustment and rotation unit to expand the ventilation coverage area.

[0018] Compared with the prior art, the beneficial effects of the present invention are:

[0019] (1) When the angle-adjusting gear rotates around the drive shaft, it drives the second bevel gear to rotate back and forth, so that it meshes with the first bevel gear on the drive seat to rotate back and forth. When the first bevel gear rotates, it drives the hollow block in the drive seat to rotate up and down. This causes the jet head on the hollow block to swing up and down while rotating left and right, thereby increasing the working range of the jet head and increasing its working angle. This further increases the working range of the jet head, enabling it to discharge exhaust gas and deliver sterile air to different ranges and heights on several shelves in the incubator. This allows the device to target different levels of bacteria in the incubator, avoiding dead corners in ventilation. This ensures that exhaust gas in the bacteria area can be discharged smoothly and oxygen supply is sufficient. This not only reduces ventilation limitations but also improves selenium conversion efficiency, allowing the bacteria to be uniformly enriched with selenium, ultimately improving the incubation effect of the device.

[0020] (2) The irrigation pipes on the driven gear and the driving gear reciprocate within the plate to spray the nutrient solution in the irrigation pipe onto the cultivated bacteria, providing the necessary "energy and material basis" for the growth, reproduction and selenium metabolism of the bacteria, meeting the selenium enrichment requirements, improving the selenium conversion efficiency, and thus improving the cultivation effect of the device. At the same time, the stirring rod on the irrigation pipe continuously stirs the bacteria during the reciprocating rotation, which disperses the bacteria and ensures that the nutrient solution is evenly applied to the bacteria, improving the absorption effect. It also breaks up the layering of the bacteria and smoothly discharges the waste gases such as carbon dioxide and ammonia squeezed at the bottom of the bacteria, avoiding local hypoxia that could poison the bacteria. This allows the bacteria to fully contact the sterile air that is delivered, improving the oxygen utilization rate and the uniformity of selenium conversion, further improving the cultivation effect of the device, avoiding dead corners when the bacteria are enriched with selenium, and thus enabling the bacteria to be evenly enriched with selenium.

[0021] (3) One gas generator continuously supplies sterile gas into the incubator, while the other gas generator continuously discharges the waste gas generated during the incubation process to the outside. This prevents oxygen deficiency in the incubator due to waste gas accumulation during incubation, thereby improving the activity of the bacteria and the efficiency of selenium conversion. At the same time, sterile air is continuously supplied to meet the respiratory needs of the bacteria, preventing growth inhibition or death due to lack of oxygen during incubation. This allows the device to continuously perform active ventilation during incubation, dissipating waste gases such as carbon dioxide and ammonia produced by bacterial metabolism, preventing the accumulation of waste gas that could harm the bacteria, improving the incubation environment, regulating humidity and temperature, removing excess moisture, dissipating fermentation heat, and maintaining stable incubation conditions. This promotes contact between the bacteria and the selenium source and nutrients, improves the efficiency of selenium conversion, and makes the bacteria more uniformly enriched with selenium. Furthermore, by operating the two gas generators separately, the processes of supplying sterile air and discharging waste gas are separated, avoiding cross-contamination and further improving the incubation effect of the device, thus reducing its limitations. Attached Figure Description

[0022] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof.

[0023] In the attached diagram:

[0024] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0025] Figure 2 This is a schematic diagram of the internal structure of the incubator of the present invention;

[0026] Figure 3 This is a schematic diagram of the air intake pipe structure of the present invention;

[0027] Figure 4 This is a schematic diagram of the L-shaped flexible tube structure of the present invention;

[0028] Figure 5 This is a cross-sectional view of the gas-generating box of the present invention;

[0029] Figure 6 This is a cross-sectional view of the hollow circular block of the present invention;

[0030] Figure 7 This is a schematic diagram of the gas-generating square plate structure of the present invention;

[0031] Figure 8 This is a schematic diagram of the positioning rack structure of the present invention;

[0032] Figure 9 This is a schematic diagram of the jet head structure of the present invention;

[0033] Figure 10 This is a schematic diagram of the driving seat structure of the present invention;

[0034] In the diagram: 1. Incubator; 2. Shelf; 3. Gas generator box; 4. Rotating disc; 5. Rotating cylinder; 6. Drive gear; 7. Drive base; 8. Drive shaft; 9. Drive base; 10. Hollow block; 11. Jet nozzle; 12. First bevel gear; 13. First base; 14. Adjusting gear; 15. Second bevel gear; 16. Half-cut gear ring; 17. Transfer tube; 18. First bend tube; 19. L-shaped hose; 20. Gas outlet nozzle; 21. Air inlet pipe; 22. None 23. Air filter; 24. Third bevel tooth; 25. Fourth bevel tooth; 26. Second base; 27. Positioning cylinder; 28. Rectangular cross block; 29. ​​Rectangular slide groove; 30. Positioning rack; 31. Positioning gear; 32. Driven gear; 33. Irrigation pipe; 34. Stirring rod; 35. Third base; 36. Guide cylinder; 37. Guide cross block; 38. Gas generating plate; 39. Drive motor; 40. Drive cam; 41. Guide spring; 42. Rectangular toothed frame. Detailed Implementation

[0035] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0036] Implementation examples, by Figures 1 to 10The present invention includes an incubator 1; the incubator 1 is provided with several shelf plates 2, on which the inoculum is placed; the incubator 1 is provided with a gas-generating device for targeted operation on inoculum at different levels; the gas-generating device includes a gas-generating box 3, which is installed on the top of the incubator 1; two gas-generating boxes 3 are symmetrically arranged with their openings facing each other; a rotating disc 4 is installed on the outer wall of the incubator 1; a re-moving stirring component is provided on the rotating disc 4 for dispersing the inoculum; a drive gear 6 is installed on the top of the incubator 1; an angle-adjusting rotation unit is provided on the drive gear 6 for increasing the ventilation range; a transfer pipe 17 is installed on the side wall of the incubator 1; two transfer pipes 17 are symmetrically arranged; and a first bend is installed on the top of each of the two gas-generating boxes 3. Pipe 18; the two first bent pipes 18 are connected to two intermediate round pipes 17 at their ends away from the gas generating box 3; the first bent pipes 18 and the gas generating box 3 are connected to the intermediate round pipes 17; L-shaped hoses 19 are installed on the side wall of the intermediate round pipes 17; the number and position of the L-shaped hoses 19 correspond to the drive seat 9; one end of the L-shaped hose 19 is connected to the intermediate round pipe 17, and the other end passes through the side wall of the drive seat 9 and is located inside the hollow round block 10; the hollow round block 10 and the L-shaped hoses 19 are connected; an exhaust nozzle 20 and an intake pipe 21 are installed on the opposite side of the gas generating box 3; the exhaust nozzle 20 and the intake pipe 21 correspond one-to-one with the two gas generating boxes 3; one gas generating box 3 is equipped with valve group A, and the other gas generating box 3 is equipped with valve group B. Valve group A includes a first valve port and a second valve port; the first valve port is connected to the air outlet nozzle 20, and the second valve port is connected to one of the first bend pipes 18; Valve group B includes a third valve port and a fourth valve port; the third valve port is connected to the air inlet pipe 21, and the fourth valve port is connected to the other first bend pipe 18; a sterile air filter 22 is installed on the incubator 1; the end of the air inlet pipe 21 away from the gas generating box 3 is connected to the sterile air filter 22; two symmetrical third bases 35 are provided on the top of the incubator 1; the two third bases 35 are located between the opposite openings of the two gas generating boxes 3; a through guide cylinder 36 is provided on the side of the third base 35 near the gas generating box 3; the guide cylinder 36 and the third base 35 are slidably fitted; one end of the guide cylinder 36 is connected to A guide block 37 is provided, with a gas-generating plate 38 connected to the other end. The gas-generating plate 38 is fitted into the gas-generating box 3, and the two are slidably connected. A drive motor 39 is installed on the top of the incubator 1. A drive cam 40 is installed at the output end of the drive motor 39. The side of the guide block 37 away from the gas-generating plate 38 is located on the rotation path of the side wall of the drive cam 40. The two guide blocks 37 are located on both sides of the drive cam 40 and are symmetrically arranged with the center of the drive cam 40 as the axis of symmetry. A guide spring 41 is sleeved on the guide cylinder 36. One end of the guide spring 41 is connected to the third base 35, and the other end is connected to the guide block 37. A rectangular gear frame 42 is installed on the guide block 37. The drive gear 6 is located in the rectangular gear frame 42, and the two are meshed.

[0037] The drive motor 39 is started, causing its output end to drive the drive cam 40 to rotate. This causes the cam's sidewall to continuously contact the two guide blocks 37. Specifically, it contacts one guide block 37 first, then the other, repeating this process to ensure the two guide blocks 37 operate in a staggered manner. When the guide block 37 is contacted by the rotating drive cam 40's sidewall, the guide cylinder 36 on the guide block 37 moves to a limited position at the third base 35, putting the guide spring 41 in a buffered state. When the guide block 37 no longer contacts the drive cam 40, the guide spring 41 returns to its original position due to the loss of force, thus resetting the guide block 37. The movement causes the guide block 37 to move back and forth within the gas generating box 3 under the action of the guide cylinder 36, driving the gas generating plate 38 to move reciprocally. The operating process for one of the gas generating boxes 3 is as follows: when the gas generating plate 38 moves close to the air inlet pipe 21, the third valve port closes and the fourth valve port opens, pushing the sterile gas within the gas generating box 3 into one of the first bent pipes 18. This gas is then transported by the transfer pipe 17 and several L-shaped hoses 19 to several hollow round blocks 10, where the jet nozzles 11 on the hollow round blocks 10 spray the delivered sterile gas onto the bacterial cultures on several shelf plates 2. When the gas generating plate 38 returns to its original position, the drive cam 4... 0 is not in contact with the two guide blocks 37. At this time, the fourth valve is closed and the third valve is open, so that the suction force in the gas generating box 3 acts on the air inlet pipe 21 to draw the sterile gas generated at the sterile air filter 22 into the gas generating box 3. The gas generating plate 38 in the gas generating box 3 then moves to continuously deliver sterile gas to several layers of bacteria in the incubator 1. At this time, by continuing to rotate the drive cam 40, another gas generating box 3 starts to operate. The specific operation process is as follows: the gas generating plate 38 in the gas generating box 3 moves closer to the air outlet nozzle 20. At this time, the first valve opens and the second valve closes, so that the gas generating plate 3 in the gas generating box 3 moves closer to the air outlet nozzle 20. The gas inside the gas-generating box 3 is discharged to the outside through the gas outlet nozzle 20. At this time, the drive cam 40 does not contact the two guide blocks 37, so that the gas-generating plate 38 is reset and moved. At this time, the first valve is closed and the second valve is opened, so that the suction force inside the gas-generating box 3 acts on another first bend pipe 18. Under the action of the transfer round pipe 17, several L-shaped hoses 19 and hollow round blocks 10, the exhaust gas and harmful gas generated during the cultivation of bacteria on several layers of shelf 2 in the incubator 1 are sucked into the gas-generating box 3 through the jet nozzle 11 on the hollow round block 10, and wait for the next movement of the gas-generating plate 38 at the gas-generating box 3 for discharge.One gas generator 3 continuously supplies sterile gas into the incubator 1, while the other continuously exhausts waste gas generated during the microbial cultivation process in incubator 1 to the outside. This prevents oxygen depletion in incubator 1 due to waste gas accumulation, thus improving microbial activity and selenium conversion efficiency. Simultaneously, the continuous supply of sterile air meets the respiration needs of the microorganisms, preventing growth inhibition or death due to oxygen deficiency during cultivation. This allows for continuous active ventilation during microbial cultivation, removing waste gases such as carbon dioxide and ammonia produced by microbial metabolism, preventing the accumulation of toxic gases that could harm the microorganisms. It also improves the cultivation environment, regulates humidity and temperature, removes excess moisture, dissipates fermentation heat, and maintains stable cultivation conditions. This promotes contact between the microorganisms and the selenium source and nutrients, increasing selenium conversion efficiency and resulting in more uniform selenium enrichment of the microorganisms. Furthermore, by staggering the operation of the two gas generators 3, the processes of supplying sterile air and emitting waste gas are separated, preventing cross-contamination and further enhancing the cultivation effect of the device, thus reducing its limitations.

[0038] The compound stirring assembly of this embodiment includes a rotating column 5, which is installed on the edge of the rotating disk 4 away from the incubator 1; a third bevel gear 23 is installed on the side of the drive base 7 away from the drive shaft 8; the third bevel gear 23 and the drive shaft 8 are rotatably connected; a fourth bevel gear 24 is installed inside the incubator 1; the fourth bevel gear 24 is rotatably connected to the rotating disk 4; the fourth bevel gear 24 is meshed with the third bevel gear 23; a second base 25 is installed on the outer wall of the incubator 1; a through positioning cylinder 26 is provided on the side of the second base 25 near the rotating column 5; the positioning cylinder 26 and the second base 25 are slidably engaged; a rectangular horizontal block 27 is installed on the positioning cylinder 26; the rectangular horizontal block 27 is located on the side of the rotating disk 4 away from the incubator 1; the rectangular horizontal block A rectangular groove 28 is provided on the side of the rotating disc 4; the rotating column 5 is located in the rectangular groove 28, and the two slide in fit; a positioning rack 29 is provided on the side of the rectangular horizontal block 27 away from the positioning cylinder 26; a positioning gear 30 is installed on the side wall of the incubator 1; the positioning gear 30 is meshed with the positioning rack 29; a drive gear 31 is installed on the positioning gear 30, and the two are rotatably connected; the drive gear 31 is installed on the inner wall of the incubator 1; driven gears 32 are meshed on both sides of the drive gear 31; driven gears 32 are installed on the inner wall of the incubator 1; irrigation pipes 33 are connected to both the driven gear 32 and the drive gear 31; the irrigation pipes 33 are located inside the shelf 2; a stirring rod 34 is installed on the outer wall of the irrigation pipes 33;

[0039] When the angle adjustment and positioning unit controls the working range and angle of the jet head 11, it causes the drive shaft 8 to rotate, which in turn drives the third bevel gear 23 to rotate, causing it to mesh with the fourth bevel gear 24. The fourth bevel gear 24, in turn, synchronously drives the rotating disk 4 to rotate, causing the rotating column 5 on it to reciprocate within the rectangular slide groove 28. This causes the rectangular horizontal block 27 on it to reciprocate at the second base 25 via the positioning cylinder 26. The rectangular horizontal block 27 then drives the positioning rack 29 to reciprocate, causing it to mesh with the positioning gear 30 and rotate. This, in turn, drives the drive gear 31 to rotate, synchronously meshing with the driven gear 32. This causes the irrigation pipes 33 on both the driven gear 32 and the drive gear 31 to reciprocate within the placement plate 2, used to irrigate... The nutrient solution in the irrigation pipe 33 is sprayed into the cultivated microorganisms, providing the necessary "energy and material basis" for their growth, reproduction, and selenium enrichment metabolism, meeting their selenium enrichment needs, improving selenium conversion efficiency, and thus enhancing the cultivation effect of the device. Simultaneously, the reciprocating rotation of the irrigation pipe 33 causes the stirring rod 34 to continuously agitate the microorganisms, breaking them apart and ensuring the nutrient solution is evenly distributed within them, improving absorption. This also breaks up the stratification of the microorganisms, allowing waste gases such as carbon dioxide and ammonia squeezed out from the bottom to be discharged smoothly, preventing localized oxygen deficiency that could harm the microorganisms. This ensures the microorganisms can fully contact the incoming sterile air, improving oxygen utilization and selenium conversion uniformity, further enhancing the cultivation effect of the device, avoiding dead zones during selenium enrichment, and ensuring uniform selenium enrichment of the microorganisms.

[0040] The angle adjustment and positioning unit of this embodiment includes a drive base 7, which is installed inside the incubator 1; a drive shaft 8 is installed on the drive base 7; the drive shaft 8 is rotatably connected to the drive gear 6; a drive square seat 9 is installed on the drive shaft 8; the number of drive square seats 9 is the same as that of the shelf 2, and each drive square seat 9 is located above the shelf 2; a hollow round block 10 is rotatably connected inside the drive square seat 9; an air jet head 11 is installed on the outer wall of the hollow round block 10, and the two are connected; a side of the drive square seat 9 is equipped with The first bevel tooth 12 is rotatably connected to the hollow circular block 10; a first base 13 is mounted on the drive square base 9; an angle-adjusting gear 14 is mounted on the top of the first base 13; a second bevel tooth 15 is mounted on the bottom of the first base 13; the second bevel tooth 15 and the angle-adjusting gear 14 are rotatably connected; the second bevel tooth 15 and the first bevel tooth 12 are meshed; a half-cut toothed ring 16 is installed inside the incubation box 1; the half-cut toothed ring 16 is meshed with the angle-adjusting gear 14; the half-cut toothed ring 16 and the center of the drive shaft 8 are coaxial;

[0041] The reciprocating movement of the guide block 37 also synchronously drives the rectangular gear frame 42 to reciprocate, causing the internal drive gear 6 to continuously mesh and rotate, thus causing the drive shaft 8 to rotate reciprocally. The jet head 11, which delivers sterile air and exhausts waste gas, is mounted on the drive seat 9 via a hollow round block 10. This causes the drive shaft 8 to drive the jet head 11 on the drive seat 9 to rotate left and right continuously, enabling it to operate in different positions and increasing its working range. Simultaneously, when the jet head 11 on the drive seat 9 swings left and right, the adjusting gear 14 on the drive seat 9 continuously reciprocates around the half-tooth ring 16. The two are meshed, causing the adjusting gear 14 to rotate on its own axis while rotating around the drive shaft 8. This reciprocating rotation of the adjusting gear 14 drives the second bevel gear 15 to reciprocate, causing it to mesh with the drive seat 9. The first umbrella tooth 12 on the upper part rotates back and forth, causing the hollow circular block 10 inside the drive seat 9 to rotate up and down. This causes the jet head 11 on the hollow circular block 10 to swing up and down while rotating left and right, thereby increasing the working range and working angle of the jet head 11. This further increases the working range of the jet head 11, enabling it to discharge exhaust gas and deliver sterile air to different ranges and heights on several layers of shelves 2 in the incubator 1. This allows the device to target different levels of bacteria in the incubator 1, avoiding dead zones in ventilation. It ensures that exhaust gas in the bacteria area can be discharged smoothly and oxygen supply is sufficient, which not only reduces ventilation limitations but also improves selenium conversion efficiency, allowing the bacteria to be uniformly enriched with selenium, ultimately improving the cultivation effect of the device.

[0042] This invention also provides a method for cultivating selenium-enriched microbial fertilizer strains, comprising the following steps:

[0043] S1. Place the inoculum to be cultivated on the shelf 2 so that the inoculum is evenly enriched with selenium in the incubator 1.

[0044] S2. Operate the gas-generating device to perform targeted operations on bacteria at different levels and heights;

[0045] S3. The bacteria are dispersed by the compound stirring component to avoid the waste gas being unable to be discharged due to the accumulation and compression of bacteria;

[0046] S4. Control the angle adjustment and rotation unit to expand the ventilation coverage area.

[0047] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0048] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for cultivating microbial strains for selenium-enriched fertilizer, comprising an incubation box; characterized in that: The incubation chamber is equipped with several shelf units on which the inoculum is placed. The incubation chamber is equipped with a gas-generating device for targeted treatment of inoculum at different levels. The gas-generating device includes a gas-generating box mounted on the top of the incubation chamber; two gas-generating boxes are symmetrically arranged with their openings facing each other. A rotating disc is mounted on the outer wall of the incubation chamber; a re-moving agitator is mounted on the rotating disc to disperse the inoculum; the re-moving agitator includes a rotating column mounted on the edge of the rotating disc away from the incubation chamber. A drive gear is mounted on the top of the incubation chamber; an angle-adjusting unit is mounted on the drive gear to increase the ventilation range; the angle-adjusting unit includes a drive base mounted inside the incubation chamber; a drive shaft is mounted on the drive base; the drive shaft is rotatably connected to the drive gear. A drive seat is mounted on the drive shaft; the number of drive seats is the same as the shelf, and each drive seat is located above the shelf; a hollow block is rotatably connected inside the drive seat; an air jet is mounted on the outer wall of the hollow block, and the two are connected. The drive square base is equipped with a first bevel gear on its side, which is rotatably connected to the hollow circular block; a first base is installed on the drive square base; an angle-adjusting gear is installed on the top of the first base; a second bevel gear is installed on the bottom of the first base; the second bevel gear and the angle-adjusting gear are rotatably connected; the second bevel gear and the first bevel gear are meshed; a half-cut toothed ring is installed inside the incubation box; the half-cut toothed ring is meshed with the angle-adjusting gear; the center of the half-cut toothed ring and the drive shaft are coaxial. The two gas generating boxes are respectively equipped with gas outlet nozzles and gas inlet pipes on opposite sides; the gas outlet nozzles and gas inlet pipes correspond one-to-one with the two gas generating boxes; one gas generating box is equipped with valve group A, and the other gas generating box is equipped with valve group B; valve group A includes a first valve port and a second valve port. The first valve port is connected to the air outlet nozzle, and the second valve port is connected to one of the first bend pipes; valve group B includes a third valve port and a fourth valve port; the third valve port is connected to the air inlet pipe, and the fourth valve port is connected to another first bend pipe; a sterile air filter is installed on the incubator; the end of the air inlet pipe away from the gas generating box is connected to the sterile air filter. A third bevel tooth is installed on the side of the drive base away from the drive shaft; the third bevel tooth is rotatably connected to the drive shaft; a fourth bevel tooth is installed inside the incubation box; the fourth bevel tooth is rotatably connected to the rotating disk; the fourth bevel tooth meshes with the third bevel tooth; a second base is installed on the outer wall of the incubation box; a through positioning cylinder is provided on the side of the second base near the rotating column; the positioning cylinder and the second base are slidably fitted; a rectangular horizontal block is installed on the positioning cylinder; the rectangular horizontal block is located on the side of the rotating disk away from the incubation box; a through rectangular groove is provided on the side of the rectangular horizontal block near the rotating disk; the rotating column is located in the rectangular groove, and the two are slidably fitted; a positioning rack is provided on the side of the rectangular horizontal block away from the positioning cylinder.

2. The device for cultivating selenium-enriched microbial fertilizer strains according to claim 1, characterized in that: The incubator has a transfer tube installed on its side wall; two transfer tubes are symmetrically arranged; a first bend tube is installed on the top of each of the two gas generating boxes; the ends of the two first bend tubes away from the gas generating boxes are respectively connected to the two transfer tubes; the first bend tubes and the gas generating boxes are connected to the transfer tubes; an L-shaped hose is installed on the side wall of the transfer tube; the number and position of the L-shaped hoses correspond to the drive seat; one end of the L-shaped hose is connected to the transfer tube, and the other end passes through the side wall of the drive seat and is located inside the hollow block; the hollow block is connected to the L-shaped hose.

3. The device for cultivating selenium-enriched microbial fertilizer strains according to claim 1, characterized in that: A positioning gear is installed on the side wall of the incubator; the positioning gear meshes with a positioning rack; a driving gear is installed on the positioning gear, and the two are rotatably connected; the driving gear is installed on the inner wall of the incubator; driven gears are meshed on both sides of the driving gear; the driven gears are installed on the inner wall of the incubator; irrigation pipes are connected to both the driven gear and the driving gear; the irrigation pipes are located inside the shelf; a stirring rod is installed on the outer wall of the irrigation pipes.

4. The microbial inoculum cultivation device for selenium-enriched fertilizer according to claim 1, characterized in that: The top of the incubator is provided with two symmetrical third bases; the two third bases are located between the openings of the two gas generating boxes; a through guide cylinder is provided on the side of the third base near the gas generating box; the guide cylinder and the third base are slidably engaged; one end of the guide cylinder is connected to a guide block, and the other end is connected to a gas generating plate; the gas generating plate is fitted into the gas generating box, and the two are slidably engaged.

5. The device for cultivating selenium-enriched microbial fertilizer strains according to claim 4, characterized in that: A drive motor is installed on the top of the incubator; a drive cam is installed at the output end of the drive motor; the guide block is located on the rotation path of the drive cam sidewall on the side away from the gas-generating plate; two guide blocks are located on both sides of the drive cam and are symmetrically arranged with the center of the drive cam as the axis of symmetry; a guide spring is sleeved on the guide cylinder; one end of the guide spring is connected to the third base and the other end is connected to the guide block; a rectangular gear frame is installed on the guide block; the drive gear is located inside the rectangular gear frame and the two are meshed together.

6. A method for cultivating selenium-enriched microbial fertilizer strains, using the microbial fertilizer strain cultivation device as described in claim 1, characterized in that, Including the following steps: S1. Place the inoculum to be cultivated on the shelf to ensure that the inoculum is evenly enriched with selenium in the incubator. S2. Operate the gas-generating device to perform targeted operations on bacteria at different levels and heights; S3. The bacteria are dispersed by the compound stirring component to avoid the waste gas being unable to be discharged due to the accumulation and compression of bacteria; S4. Control the angle adjustment and rotation unit to expand the ventilation coverage area.

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