Cyanide-reducing bio-adsorbent as well as preparation method and application thereof

Abstract

The invention discloses a cyanide-reducing bio-adsorbent as well as a preparation method and application thereof, and relates to the technical field of mining wastewater treatment. The cyanide-reducing bio-adsorbent comprises paenibacillus and an immobilized carrier, the paenibacillus is fixed on the immobilization carrier; the bacillus is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 14, 2025, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 34545. The cyanide-reducing bio-adsorbent provided by the invention can be used for adsorbing and treating cyanide in mining wastewater, and has the advantages of high adsorption rate, stable structure, high adsorption capacity, low cost, recyclability and the like.

Description

Technical Field

[0001] This invention relates to the field of mining wastewater treatment technology, and in particular to a cyanide-reducing biosorbent, its preparation method, and its application. Background Technology

[0002] Cyanide-containing wastewater is a byproduct of metal beneficiation and refining processes. Its greatest hazard lies in the residual cyanide and heavy metal complex ions, which can generate highly toxic cyanide ions (CN). - Cyanide residues mainly consist of three categories: firstly, free cyanide, i.e., CN2, hydrogen cyanide ... - The first category is HCN; the second is simple cyanides, which are cyanides that can be dissociated by weak acids; the third category is complex and stable cyanides, namely complex alkali metal-metal cyanides.

[0003] Currently, the main technologies for treating cyanide-containing wastewater both domestically and internationally include: chemical oxidation, physical adsorption, electrochemical methods, natural degradation, and microbial methods. Natural degradation converts cyanide into less toxic cyanate through natural volatilization, hydrolysis, and photodegradation and dissociation by sunlight. This method is low-cost and does not produce toxic byproducts, but its treatment efficiency is low and requires sufficient retention time. Chemical oxidation methods mainly include hydrogen peroxide, basic chlorination, and acidification recovery. This technology is relatively mature, with fast reaction speeds and short process cycles, but the large consumption of reagents increases costs, and residual chlorine in the treated wastewater can pose a threat to aquatic organisms. Physical adsorption methods mainly include ion exchange, activated carbon adsorption, and air flotation. These methods are technologically mature, simple to operate, and allow for the reuse of recovered cyanide and heavy metals. However, a drawback is that the integrated equipment cannot be reused, easily causing secondary pollution. Electrochemical methods can treat toxic, harmful, and recalcitrant mining wastewater through electrocatalytic oxidation, electrocoagulation, electroadsorption, and electroosmosis. They offer advantages such as simple operation and small equipment size. However, they can easily increase the heavy metal load in the wastewater, have low reactor efficiency, suffer from severe electrode wear, and have high energy consumption. Microbial methods involve adding microbial agents to the water treatment equipment. The combined use of microbial agents has lower requirements for the aquatic environment, a wider range of applications, better economic benefits, and advantages such as simple process for recovering elemental substances from the water, high removal efficiency, environmental friendliness, and no secondary pollution.

[0004] This invention aims to develop a novel composite biosorbent to improve the treatment efficiency of complex cyanide-containing wastewater. Summary of the Invention

[0005] The purpose of this invention is to provide a cyanide-reducing biosorbent, its preparation method, and its application, to solve the problems existing in the prior art. This cyanide-reducing biosorbent can be used to adsorb and treat cyanide in mining wastewater, and has advantages such as high adsorption rate, stable structure, high adsorption capacity, low cost, and recyclability.

[0006] To achieve the above objectives, the present invention provides the following solution:

[0007] This invention provides a Paenibacillus sp. with cyanide tolerance and degradation activity. The Paenibacillus sp. was deposited on May 14, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 34545.

[0008] The present invention also provides the application of the above-mentioned Bacillus subtilis in the preparation of microbial preparations for degrading cyanide.

[0009] The present invention also provides a microbial preparation for degrading cyanide, wherein the active ingredient includes the above-mentioned Bacillus subtilis.

[0010] The present invention also provides the application of the above-mentioned Bacillus-like or microbial preparations in the degradation of cyanide.

[0011] The present invention also provides a cyanide-reducing biosorbent, comprising the above-mentioned Bacillus subtilis and an immobilized carrier; wherein the Bacillus subtilis is immobilized on the immobilized carrier.

[0012] Furthermore, the immobilization carrier is sodium-based bentonite.

[0013] The present invention also provides a method for preparing the above-mentioned cyanide-reducing biosorbent, comprising the following steps:

[0014] The bacterial suspension of the above-mentioned Bacillus subtilis is mixed with the immobilization carrier to immobilize the Bacillus subtilis on the immobilization carrier, thereby obtaining a mixture of immobilized biosorbents.

[0015] After the immobilized biosorbent mixture is mixed evenly with the freeze-drying protectant, the cyanide-reducing biosorbent is obtained by freeze-drying.

[0016] Furthermore, the freeze-drying protectant includes trehalose, skim milk, and glycerin.

[0017] The present invention also provides the application of the above-mentioned cyanide-reducing biosorbent in reducing the cyanide content of cyanide-containing wastewater.

[0018] The present invention also provides a method for treating complex cyanide-containing wastewater, comprising the step of adding the above-mentioned cyanide-reducing biosorbent to the cyanide-containing wastewater for wastewater treatment to reduce the cyanide content in the cyanide-containing wastewater.

[0019] The present invention discloses the following technical effects:

[0020] This invention isolates a strain of Paenibacillus sp. SH-5, possessing cyanide tolerance and degradation activity, from activated sludge in municipal wastewater treatment plants. By immobilizing Paenibacillus sp. SH-5 in natural minerals, this invention forms a highly efficient cyanide-reducing biosorbent, which can be used to adsorb and treat cyanide in mining wastewater. This biosorbent has advantages such as high adsorption rate, stable structure, high adsorption capacity, low cost, and recyclability.

[0021] This invention, through its combination with natural minerals, overcomes the limitations imposed by environmental factors on the adsorption of live bacterial strains as biosorbents, effectively improving the storage stability of the bacterial agent and expanding its application scope. The biosorbent provided by this invention has great application potential in the treatment of cyanide-containing mining wastewater. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a colony morphology diagram of strain SH-5;

[0024] Figure 2 Microscopic image of strain SH-5;

[0025] Figure 3 Agarose gel electrophoresis image of PCR products of strain SH-5;

[0026] Figure 4 Phylogenetic tree of strain SH-5;

[0027] Figure 5 To reduce the cyanide content of strain SH-5 against CN at different treatment times - A statistical chart of removal rates;

[0028] Figure 6 To reduce cyanide levels in C2C biosorbents and sodium-based bentonite adsorbents under different treatment time conditions, the effects of these adsorbents on CN2C were investigated. - A statistical chart of removal rates;

[0029] Figure 7 To reduce the cyanide concentration of biosorbents for CN at different initial concentrations - A statistical chart of removal rate and adsorption capacity;

[0030] Figure 8 To test the adsorption effect of cyanide-reducing biosorbents using a laboratory simulation device, CN - A statistical chart of removal rates. Detailed Implementation

[0031] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0032] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0033] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0034] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be obvious to those skilled in the art. This specification and embodiments are merely exemplary.

[0035] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0036] Example 1

[0037] 1. Strains Isolation

[0038] This invention uses a cyanide screening medium to isolate a bacterium with cyanide tolerance and degradation activity from activated sludge in a municipal wastewater treatment plant, named SH-5.

[0039] Screening medium formulation (g / L): Agar 20.0 g / L, Ammonium sulfate 0.4 g / L, Sodium chloride 0.1 g / L, Potassium dihydrogen phosphate 0.4 g / L, Glucose 0.04 g / L, Yeast extract 1.0 g / L, with the addition of mining wastewater (CN... - (Concentration 10~50 mg / L).

[0040] 2. Strain identification

[0041] (1) Morphological identification

[0042] Single colonies of strain SH-5 were picked and inoculated onto nutrient agar medium, placed in a constant temperature incubator at 30 ℃, and incubated for 24 h. The colony morphology of the strain was then observed. Figure 1 As shown, the colonies are pale yellow, translucent, flat, and moist.

[0043] (2) Physiological and biochemical identification

[0044] Strain SH-5 stains purple with Gram stain, indicating it is a Gram-negative bacterium with a short rod-like morphology. Figure 2 ).

[0045] (3) Molecular biological identification

[0046] The genome of strain SH-5 was extracted and its 16S rDNA was prepared for PCR amplification. Universal primers were used: forward primer fd1 (27F): 5'-AGAGTTTGATCCTGGCTCAC-3' (SEQ ID NO.1); reverse primer rp2 (1492R): 5'-ACGGCTCCTTGTTACGCTT-3' (SEQ ID NO.2).

[0047] The PCR reaction system consisted of (25 μL): 2 μL genomic DNA (80-100 ng), 1 μL forward primer fd1 (10 pmol), 1 μL reverse primer rp2 (10 pmol), 2.5 μL 10× buffer, 1.5 μL 1.5 mmol / L MgCl2, 2.0 μL 2.5 mmol / L Taq DNAase, and ddH2O to a final volume of 25 μL.

[0048] PCR reaction conditions were as follows: 95℃ pre-denaturation for 4 min; 94℃ denaturation for 1 min, 48℃ annealing for 1 min, 72℃ extension for 1 min, 35 cycles; 72℃ for 10 min, and storage at 4℃. Detection was performed by 2% agarose gel electrophoresis.

[0049] The results are as follows Figure 3 This indicates that the 16S rDNA band of strain SH-5 is between 1000 bp and 2000 bp.

[0050] The PCR products were purified and recovered using the UNIQ-10 column DNA gel extraction kit (Shanghai Sangon Biotech) and sent to Suzhou Genewiz Biotechnology Co., Ltd. for sequencing. The sequencing results are shown in SEQ ID NO.3.

[0051]

[0052] The sequences obtained after sequencing strain SH-5 were compared with those obtained in NCBI using BLAST software for similarity search. Multiple sequence alignment analysis was performed using Clustal X software. A phylogenetic tree was constructed using the NJ method with MEGA 7.0 software to determine the taxonomic position of the bacteria.

[0053] like Figure 4 As shown, strain SH-5 (marked with black dots in the figure) and Paenibacillus sp. strain 83O (accession number PP259472.1) form a highly supported clade (100% support), indicating a very high evolutionary correlation between the two. Further analysis revealed that the phylogenetic cluster to which this clade belongs also includes several other species such as Paenibacillus lautus (NR_112724.1), Paenibacillus solani (NR_152686.1), and Paenibacillus purispatii (NR_116496.1), with high support rates (91% and 84%, respectively), indicating that SH-5 stably belongs to the genus Paenibacillus. As an outgroup, Priestia aryabhattai (NR_115953.1) is placed separately at the bottom of the tree, supporting the overall root orientation of the phylogenetic tree and validating the rationality of the phylogenetic relationships within the genus Paenibacillus.

[0054] In summary, the identification results show that strain SH-5 belongs to Paenibacillus sp.

[0055] 3. Preservation of microbial strains

[0056] Paenibacillus sp. SH-5 was deposited on May 14, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 34545.

[0057] Example 2

[0058] 1. Preparation of fermentation broth

[0059] Single colonies of strain SH-5 were picked from the solid culture medium and inoculated into the fermentation medium. Fermentation was carried out in a constant temperature shaking incubator to obtain the fermentation broth. Fermentation conditions: temperature 30 ℃, shaking speed 160 rpm, overnight incubation, OD obtained. 600 The fermentation broth is 0.5.

[0060] Fermentation medium formulation: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, pH 7.0.

[0061] 2. Preparation of cyanide-containing wastewater:

[0062] The high-cyanide wastewater was allowed to stand, and the supernatant was taken and diluted with tap water to obtain cyanide-containing wastewater with a total cyanide content of 100 mg / L.

[0063] 3. Add the fermentation broth obtained in step 1 to 100 mL of cyanide-containing wastewater with a total cyanide content of 100 mg / L at a dosage of 10 mL / L. Place the mixture in a constant temperature shaker and react for 120 h at 25 ℃ and 150 rpm. Take samples every 24 h, and perform 3 parallel tests for each time gradient. Take the average value of the results.

[0064] The method for determining cyanide is as follows:

[0065] The Hach reagent kit was used for total cyanide analysis in water quality. The sensitivity was 0.002 mg / L, the limit of detection was 0.002 mg / L, and the upper limit of detection was 0.240 mg / L. Distilled water was used to dilute the samples to the detection range before testing.

[0066] The experimental results are shown in Table 1 and Figure 5 As shown, when the fermentation broth of cyanide-reducing strain SH-5 is added at a concentration of 10 mL / L, the CN content in the cyanide-containing wastewater decreases. - At a concentration of 100 mg / L, the cyanide-reducing biosorbent has a certain effect on CN. - The removal rate increased with the increase of treatment time, reaching 19.74% at 24 h, 34.01% at 72 h, and the degradation rate tended to reach equilibrium after 96 h.

[0067] Table 1. Effects of different treatment times on CN-5 strain SH-5. - Removal effect

[0068]

[0069] Example 3

[0070] 1. Preparation of fermentation broth

[0071] Single colonies of strain SH-5 were picked from the solid culture medium and inoculated into the fermentation medium. Fermentation was carried out in a constant temperature shaking incubator to obtain the fermentation broth. Fermentation conditions: temperature 30 ℃, shaking speed 160 rpm, overnight incubation, OD obtained. 600 The fermentation broth is 0.5.

[0072] Fermentation medium formulation: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, pH 7.0.

[0073] 2. Preparation of bacterial suspension

[0074] The fermentation broth was centrifuged at high speed (8000 rpm to 10000 rpm for 6 to 10 minutes), and the supernatant was discarded to obtain bacterial cells. The bacterial cells were resuspended in sterile physiological saline, centrifuged, and the process of washing away residual culture medium from the bacterial cells was repeated three times. The bacterial cells were then resuspended in sterile distilled water to obtain a bacterial suspension. OD 600 It is 1.5.

[0075] 3. Strain fixation

[0076] Immobilization carrier: Commercially available sodium-based bentonite with a purity of 99% and particle sizes of D90 29.9 μm and D50 10.72 μm.

[0077] Sodium-based bentonite was added to the bacterial suspension at a mass ratio of 21:1. The mixture was placed in a constant-temperature shaker at 150 rpm and 25°C for 30 min. After mixing, the mixture was placed in a 4°C refrigerator for 24 h to immobilize the bacterial strain SH-5 onto the sodium-based bentonite carrier, thus obtaining an immobilized biosorbent mixture.

[0078] The immobilized biosorbent mixture was mixed with a freeze-drying protectant (trehalose, skim milk, and glycerol) in a mass ratio of 1:0.12:0.12:0.03. The mixture was then freeze-dried, ground, and sieved (using a 200-mesh sieve) to obtain the final cyanide-reducing biosorbent.

[0079] Comparative Example 1

[0080] Sodium-based bentonite was added to sterile water at a mass ratio of 21:1. The mixture was placed in a constant-temperature vibrator at 150 rpm and 25°C for 30 min. After mixing, the mixture was placed in a 4°C refrigerator for 24 h to obtain a sodium-based bentonite-water mixture.

[0081] Sodium-based bentonite-water mixture: trehalose: skim milk: glycerin = 1:0.12:0.12:0.03 was mixed evenly with freeze-drying protectant (trehalose, skim milk and glycerin), and then freeze-dried, ground and sieved (200 mesh sieve) to obtain sodium-based bentonite adsorbent.

[0082] The high-cyanide wastewater involved in the following examples is mining wastewater from a gold ore beneficiation plant in Guangxi Zhuang Autonomous Region. This wastewater contains cyanide or cyanide-like ions and heavy metals. The total cyanide concentration in the wastewater was tested to be 5100 mg / L.

[0083] The method for determining cyanide is as follows:

[0084] The Hach reagent kit was used for total cyanide analysis in water quality. The sensitivity was 0.002 mg / L, the limit of detection was 0.002 mg / L, and the upper limit of detection was 0.240 mg / L. Distilled water was used to dilute the samples to the detection range before testing.

[0085] Example 4

[0086] Adsorption effect test of cyanide-reducing biosorbent (prepared in Example 3) on high cyanide wastewater:

[0087] 1. Preparation of cyanide-containing wastewater: Let the high-cyanide wastewater stand, take the supernatant after standing, and dilute it with tap water to obtain cyanide-containing wastewater with a total cyanide content of 100 mg / L.

[0088] 2. The cyanide-reducing biosorbent was added to 100 mL of cyanide-containing wastewater at a dosage of 1.5 g / L. The mixture was placed in a constant-temperature shaker and reacted at 25 ℃ and 150 rpm for 120 h. Samples were taken every 24 h, and three replicates were performed for each time gradient. The average value of the results was taken. A control group was also set up, in which the cyanide-reducing biosorbent in Example 3 was replaced with the sodium-based bentonite adsorbent prepared in Comparative Example 1.

[0089] 3. Centrifuge the obtained sample at 80,000 rpm for 10 min, collect the supernatant, and determine the cyanide content.

[0090] The experimental results are shown in Table 2 and Figure 6 As shown, when the dosage of the cyanide-reducing biosorbent is 1.5 g / L, the CN content in the cyanide-containing wastewater... - At a concentration of 100 mg / L, the cyanide-reducing biosorbent has a certain effect on CN. - The removal rate increased with increasing treatment time, reaching 30.28% after 24 hours and 69.47% after 72 hours, after which the degradation rate tended to reach equilibrium. In the comparative experiment, when the dosage of sodium-based bentonite adsorbent was 1.5 g / L, the CN content in the cyanide-containing wastewater decreased. - When the concentration was 100 mg / L, the removal rate was 21.35% after 24 hours and 22.03% after 120 hours. The removal rate was relatively stable, indicating that the removal effect of sodium-based bentonite adsorbent had reached saturation within 24 hours.

[0091] Table 2. Effects of different treatment time conditions on CN-C degradation by biosorbents and sodium-based bentonite adsorbents. - Removal rate

[0092]

[0093] Example 5

[0094] Tests on the removal efficiency of the cyanide-reducing biosorbent (prepared in Example 3) for different cyanide concentrations:

[0095] 1. Preparation of diluents for different concentrations of mineral water: The high-cyanide wastewater was allowed to stand. The supernatant was then taken and diluted with tap water according to the required concentration. After dilution, the total cyanide content was measured at 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, 80 mg / L, 100 mg / L, 120 mg / L, and 150 mg / L, with 100 mL of each solution. The pH was adjusted to 8.0 with NaOH / HCl, and the solutions were placed in 250 mL Erlenmeyer flasks. Three replicates were performed for each gradient, and the average value was taken.

[0096] 2. The cyanide-reducing biosorbent was added at a dosage of 1.5 g / L to Erlenmeyer flasks containing mineral water dilution of different concentrations, and placed in a constant temperature shaker for 72 h at 25 ℃ and 150 rpm.

[0097] 3. Centrifuge the obtained treated mineral water dilution at 80,000 rpm for 10 min, collect the supernatant, and determine the cyanide content.

[0098] The experimental results are shown in Table 3 and Figure 7 As shown, when the dosage of the cyanide-reducing biosorbent is 1.5 g / L, the CN content in the diluted mineral water solution is... - When the concentration is between 10 and 100 mg / L, the cyanide-reducing biosorbent has a certain effect on CN. - The removal rate reached over 70%, but decreased to 52.38% when the concentration was further increased to 150 mg / L. Meanwhile, the CN content in the diluted mineral water decreased... - As the concentration increases, the adsorption capacity of the cyanide-reducing biosorbent shows an upward trend.

[0099] Table 3. Effects of different initial concentrations of cyanide biosorbent on CN. - Removal effect

[0100]

[0101] Example 6

[0102] The cyanide removal efficiency of the cyanide-reducing biosorbent (prepared in Example 3) was tested using a laboratory simulation apparatus. The experimental setup consisted of an open container measuring 32 cm long, 28.5 cm wide, and 30 cm high, into which cyanide-containing wastewater was added. A vent pipe was placed in the cyanide-containing wastewater at a depth of 0.2 m. 3 Ventilation is performed at a ventilation rate of / h.

[0103] 1. Preparation of cyanide-containing wastewater: Let the high-cyanide wastewater stand, take the supernatant after standing, and dilute it with tap water to obtain 10 L of cyanide-containing wastewater with a total cyanide content of 100 mg / L.

[0104] 2. The biosorbent was added at a dosage of 1.2 g / L to a 10 L system of cyanide-containing wastewater in an open container at room temperature and an aeration rate of 0.2 m³ / L. 3 The reaction was carried out for 120 h under the condition of / h, and samples were taken every 24 h.

[0105] 3. Centrifuge the obtained sample at 80,000 rpm for 10 min, collect the supernatant, and determine the cyanide content.

[0106] Table 4. Results of cyanide removal efficiency tests using a laboratory simulation apparatus on the cyanide-reducing biosorbent.

[0107]

[0108] From Table 4 and Figure 8 The results show that in CN - The concentration was 100 mg / L, the dosage of the cyanide-reducing biosorbent was 1.2 g / L, and the ventilation rate was 0.2 m³ / L. 3 Under conditions of [temperature range missing] / h, the cyanide degradation rate increased with increasing treatment time. The cyanide-reducing biosorbent achieved a removal rate of 37.52% after 24 h and reached 71.28% after 72 h. The simulated device removed CN [missing information]. - The results were better than those of the shake-flask experiment, which may be due to the device being placed at 0.2 m. 3 Under a ventilation rate of / h, dissolved oxygen in cyanide-containing wastewater can be better increased, which helps the cyanide-reducing biosorbent to exert its adsorption effect.

[0109] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A Paenibacillus sp. having cyanide tolerance and degrading activity, characterized by, The Paenibacillus sp. is preserved in China General Microbiological Culture Collection Center on May 14, 2025, the address of the preservation center is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 34545.

2. Use of the Paenibacillus sp. of claim 1 in the preparation of a microbial preparation for degrading cyanide.

3. A microbial preparation for degrading cyanide, characterized by, The active ingredient comprises the Paenibacillus sp. of claim 1.

4. Use of the Paenibacillus sp. of claim 1 or the microbial preparation of claim 3 in degrading cyanide.

5. A cyanide-reducing biosorbent, characterized in that, The Paenibacillus sp. of claim 1 and an immobilized carrier are included; the Paenibacillus sp. is immobilized on the immobilized carrier.

6. The cyano-reducing biosorbent of claim 5, wherein, The immobilized carrier is sodium bentonite.

7. A method for the preparation of a cyano-reducing biosorbent according to claim 5 or 6, characterized in that, The method comprises the following steps: The bacterial suspension of the Paenibacillus sp. of claim 1 is mixed with the immobilized carrier for a mixed reaction, so that the Paenibacillus sp. is immobilized on the immobilized carrier to obtain an immobilized biosorbent mixture; After the immobilized biosorbent mixture is uniformly mixed with a freeze-drying protective agent, the freeze-drying protective agent is freeze-dried to obtain the cyanide-degrading biosorbent.

8. The production method according to claim 7, characterized by, The freeze-drying protective agent comprises trehalose, skimmed milk and glycerol.

9. Use of the cyanide-degrading biosorbent of claim 5 or 6 in reducing the cyanide content of cyanide-containing wastewater.

10. A method of treating cyanide-containing wastewater, characterized by, The method comprises the following steps: The cyanide-degrading biosorbent of claim 5 or 6 is added to cyanide-containing wastewater for wastewater treatment to reduce the cyanide content of the cyanide-containing wastewater.

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