Oil-control and anti-dandruff composition and application thereof

Abstract

The invention discloses an oil-control and anti-dandruff composition and application thereof, and relates to the technical field of daily chemical products. The oil-controlling and anti-dandruff composition comprises polyol, an active component A and an active component B, the active ingredient A is selected from bioflavonoid and / or plankton extract; and the active ingredient B is selected from at least one of zinc gluconate, zinc lactate, zinc PCA, zinc cocoyl alcohol polyether sulfate and zinc hyaluronate. According to the oil-control and anti-dandruff composition, the active ingredient A can effectively reduce sebum secretion and regulate the sebum level; and the active component B has good antibacterial performance, can remove biological membranes, obviously improve oily scalp and enhance scalp barrier. All the components are compounded and can achieve mutual synergistic interaction, bacteriostasis and biofilm disintegration are achieved from the source, long-acting dandruff removal and oil control are achieved, meanwhile, the scalp barrier function is improved, and scalp discomfort is relieved.

Description

Technical Field

[0001] This invention relates to the field of daily chemical product technology, and in particular to an oil-controlling and dandruff-removing composition and its application. Background Technology

[0002] Scalp health problems, especially excessive scalp oil and dandruff, plague a large number of people worldwide. The main causes are closely related to scalp microecological imbalance, abnormal sebum secretion, and damage to the scalp barrier. Traditional oil-control and dandruff-removing products mostly rely on antibacterial agents (such as zinc pyrithione and clomiphene) and keratolytic agents (such as salicylic acid and alpha-hydroxy acids) to achieve their effects.

[0003] With economic development, consumers increasingly view hair care products as an extension of scalp and skin health, and therefore hope to achieve oil control and dandruff removal through a gentle, multi-functional product.

[0004] However, existing technologies have many limitations. First, conventional antibacterial agents mainly disrupt the cell membranes of microorganisms, interfere with metabolic pathways, and inhibit protein or nucleic acid synthesis. While they can effectively reduce free and isolated microorganisms on the scalp and temporarily alleviate dandruff, they cannot effectively penetrate the external barrier of biological membranes. Second, most oil-control products rely on absorbent ingredients (such as silica, kaolin, and starch) or astringents (such as zinc oxide and alcohol), which can only temporarily absorb or astringe oil and cannot fundamentally regulate excessive sebum secretion, thus only treating the symptoms, not the root cause. In addition, frequent use of strong antibacterial agents and exfoliating ingredients can damage the lipid structure of the scalp's stratum corneum, impair the scalp's physical barrier, leading to dryness, sensitivity, and even compensatory sebum secretion, creating a vicious cycle.

[0005] Therefore, there is an urgent need in the market for an innovative solution that can effectively penetrate and dismantle the biofilm barrier of microorganisms, fundamentally inhibiting their excessive growth; simultaneously, it should regulate sebum secretion, reducing abnormal oil secretion at its source, and also repair the scalp barrier function, maintaining scalp health and stability. Currently, there is a lack of a composition that can synergistically address the three core issues of biofilm formation, abnormal sebum secretion, and impaired barrier function. This invention is formally proposed against this technological background. Summary of the Invention

[0006] To address the aforementioned technical problems, the present invention aims to overcome the shortcomings of existing technologies and provide an oil-controlling and dandruff-removing composition and its application. The components of the oil-controlling and dandruff-removing composition of the present invention work synergistically to solve three core problems: First, it effectively inhibits the formation of biofilms of scalp microorganisms such as Malassezia and Propionibacterium acnes, and dismantles existing biofilms, fundamentally solving the problems of oil control and dandruff removal; second, it regulates scalp sebum levels, reduces the number of porphyrins, and reduces scalp sebum secretion, achieving root-cause oil control; third, it effectively improves scalp barrier function, maintains a healthy and stable scalp environment, and reduces scalp inflammation, sensitivity, and dryness.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solution: In a first aspect, an oil-controlling and dandruff-removing composition is provided, comprising a polyol, active ingredient A, and active ingredient B; The active ingredient A is selected from bioflavonoids and / or plankton extracts; The active ingredient B is selected from at least one of zinc gluconate, zinc lactate, zinc PCA, zinc cocoyl ether sulfate, and zinc hyaluronic acid.

[0008] Furthermore, the oil-controlling and dandruff-removing composition comprises a polyol, active ingredient A, active ingredient B, and the remainder water.

[0009] Furthermore, the oil-controlling and dandruff-removing composition, by weight percentage, comprises 20-55% polyol, 0.2-6% active ingredient A, 2-20% active ingredient B, and the balance water.

[0010] Furthermore, the bioflavonoids are obtained by separating them from the leaves of *Echinochloa crus-galli*.

[0011] Furthermore, the polyol is selected from at least one of 1,3-propanediol, 1,2-propanediol, 1,2-butanediol, glycerol, pentanediol, and hexanediol.

[0012] Furthermore, the oil-controlling and dandruff-removing composition, by weight percentage, consists of 25-50% polyol, 0.5-5% active ingredient A, 3-15% active ingredient B, and the balance water.

[0013] Furthermore, the weight ratio of active ingredient A to active ingredient B is (0.05-1):5.

[0014] Furthermore, the weight ratio of active ingredient A to active ingredient B is (0.1-1):5.

[0015] Secondly, the application of the oil-controlling and dandruff-removing composition described in the first aspect in hair care products is provided.

[0016] Furthermore, the oil-controlling and dandruff-removing composition accounts for 0.2-10% of the weight of the shampoo and conditioner.

[0017] Preferably, the hair care product is a rinse-out type or a leave-on type.

[0018] Preferably, the oil-controlling and dandruff-removing composition accounts for 1-10% of the weight of the shampoo and conditioner.

[0019] The beneficial effects of this invention are as follows: 1) This invention provides a composition with excellent oil control and dandruff removal effects, which can be used in both rinse-out and leave-on shampoo and conditioner products.

[0020] 2) The combination of active ingredient A and active ingredient B enhances the efficacy of the composition. Active ingredient A and active ingredient B form a highly efficient synergistic system; active ingredient B clears biofilms, facilitating the penetration pathway of active ingredient A, improving its bioavailability and enhancing its efficacy, thus better regulating sebum levels; active ingredient A reduces sebum secretion at its source, effectively reducing the main lipid source for microorganisms such as Malassezia, inhibiting excessive microbial growth from the root and greatly enhancing the antibacterial effect of active ingredient B; active ingredients A and B work synergistically to improve the scalp's barrier function and improve the scalp's microecology.

[0021] 3) The composition of this invention uses polyols as excellent penetration enhancers, which can help active ingredients A and B penetrate the scalp stratum corneum more effectively and reach the target action site, thereby significantly improving the bioavailability and efficacy of active ingredients A and B; on the other hand, polyols can also provide the scalp with immediate and long-lasting moisturizing effects, reduce transepidermal water loss, thereby improving the scalp barrier function.

[0022] 4) The composition of this invention successfully achieves the triple goals of oil control, dandruff removal, and barrier repair. Its fundamental mechanism of action and synergistic effect make it a highly efficient, long-lasting, and safe comprehensive solution for scalp health problems. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1The graphs show the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to inhibit the formation of Malassezia biofilm. Figure 2 The graphs show the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to inhibit the formation of Propionibacterium acnes biofilm. Figure 3 The graphs show the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to break down the Malassezia biofilm. Figure 4 The diagram shows the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to break down the biofilm of Propionibacterium acnes. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] It should be understood that, when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, integrals, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or collections thereof.

[0027] It should also be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0028] It should also be further understood that the term "and / or" as used in this specification and the appended claims refers to any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.

[0029] To better understand the technical content of the present invention, the technical solution of the present invention will be further introduced and explained below with reference to specific embodiments.

[0030] This invention belongs to the field of cosmetic technology, specifically relating to an oil-controlling and dandruff-reducing composition and its application. The oil-controlling and dandruff-reducing composition of this invention, by weight percentage, comprises 20-50% polyol, 0.2-6% active ingredient A, 2-20% active ingredient B, and the balance water. Active ingredient A directly regulates sebaceous gland secretion, reducing excessive oil secretion at a physiological level, achieving oil control from the inside out. Active ingredient B has a certain biofilm-clearing ability, effectively inhibiting the planktonic state of microorganisms such as Malassezia and Propionibacterium acnes, and penetrating and disintegrating the protective biofilms upon which they depend for survival. This dual mechanism of biofilm disintegration and antibacterial action effectively prevents recurring dandruff and oily scalp problems.

[0031] The components of this invention are compounded to achieve synergistic effects, which helps to reduce sebum secretion, regulate scalp microbiota, and enhance scalp barrier function.

[0032] The oil-controlling and dandruff-removing composition of this invention has multiple effects, including reducing sebum secretion, inhibiting bacteria, removing dandruff, reducing inflammation, and strengthening the scalp barrier, and is gentle and non-irritating. The oil-controlling and dandruff-removing composition of this invention can be used in products such as shampoos, conditioners, hair masks, and scalp serums.

[0033] The efficacy descriptions of the main ingredients are as follows: ①Active ingredient A Active ingredient A acts directly on sebaceous gland cells, reducing excessive sebum synthesis and secretion at a physiological level by inhibiting 5α-reductase activity and regulating lipid synthesis-related signaling pathways. Furthermore, by significantly reducing scalp sebum secretion, active ingredient A fundamentally reduces the lipids required for microbial reproduction. This creates a perfect synergistic effect with active ingredient B, continuously controlling sebum levels and inhibiting microbial regeneration in the long term, greatly reducing the recurrence frequency of dandruff and acne problems. Excessive sebum secretion is easily oxidized, generating irritants such as lipid peroxides and squalene peroxides, triggering inflammation. Active ingredient A reduces the total amount of sebum, thereby alleviating inflammation, reducing scalp itching, and creating a healthier, more balanced microecological environment for the scalp.

[0034] ②Active ingredient B Active ingredient B is one of the core technologies and innovations of this invention. Through a revolutionary anti-biofilm mechanism, it solves the fundamental bottleneck of traditional dandruff removal technologies. Active ingredient B not only inhibits the growth of free microorganisms but also dismantles the protective matrix of biofilms, effectively killing stubborn bacteria hidden within the biofilm that are slow-metabolizing and insensitive to traditional antibacterial agents. This achieves long-lasting dandruff removal and prevents recurrence. By clearing biofilms, active ingredient B clears obstacles for other antibacterial agents, enhancing their efficacy. Furthermore, biofilms themselves are a strong inflammatory stimulant that continuously activates the scalp's immune response, leading to persistent itching, stinging, and discomfort. Active ingredient B's clearing of biofilms significantly relieves discomfort such as itching and burning. Active ingredient B can also clear the biofilm of Propionibacterium acnes, reducing folliculitis of the scalp caused by Propionibacterium acnes, reducing acne breakouts, and expanding the product's applicability.

[0035] ③ Polyols As excellent penetration enhancers, polyols can act on the stratum corneum of the scalp, helping active ingredients A and B to penetrate the stratum corneum more effectively, thereby significantly improving the bioavailability and efficacy of the core active ingredients. On the other hand, polyols also have synergistic antibacterial properties; they can form a synergistic antibacterial network with active ingredient B, attacking microorganisms through multiple pathways, enhancing the inhibitory effect on Malassezia, Propionibacterium acnes, etc., and reducing the risk of microbial resistance. Finally, as moisturizers, polyols can provide immediate and long-lasting hydration to the scalp, reducing transepidermal water loss and thus improving the scalp barrier.

[0036] The theoretical principle behind the synergistic effect of the above three components is as follows: 1) The polyols in the oil-controlling and dandruff-removing composition of the present invention can improve the penetration performance of active ingredients A and B, thereby significantly enhancing the bioavailability and efficacy of the abundant core active ingredients. On the other hand, it improves the scalp's barrier function.

[0037] 2) The oil-controlling and dandruff-removing composition of the present invention utilizes a combination of active ingredient A and active ingredient B, enhancing the efficacy of the composition. Active ingredient A and active ingredient B form a highly efficient synergistic system; active ingredient B clears biofilms, facilitating the penetration pathway of active ingredient A, improving its bioavailability and enhancing its efficacy, thus better regulating sebum levels; active ingredient A reduces sebum secretion at the source, which is equivalent to reducing the main lipid source for microorganisms such as Malassezia, inhibiting the excessive reproduction of microorganisms from the root, and greatly enhancing the antibacterial effect of active ingredient B; active ingredients A and active ingredient B can synergistically enhance each other, which is beneficial to improving the scalp's barrier function and improving the scalp's microecology.

[0038] 3) The components of the oil-controlling and dandruff-removing composition of the present invention work synergistically with each other, focusing on antibacterial, oil regulation, scalp barrier, and scalp microecology. Each component is indispensable, and the composition provides a comprehensive solution for efficient, long-lasting, and safe oil control and dandruff removal.

[0039] Unless otherwise specified, the experimental methods described in the following examples are generally performed under standard conditions or as recommended by the manufacturer.

[0040] Unless otherwise stated, percentages and parts are by weight, and ratios are by weight.

[0041] In the following examples, the unit of weight parts is g.

[0042] The present invention provides the following embodiments and comparative examples, wherein the total weight of the specific composition components is 100%.

[0043] The present invention does not impose any special restrictions on the preparation method of the cosmetic product; it can be prepared using methods for preparing hair care products that are well known to those skilled in the art.

[0044] In this invention, the preparation method of the oil-controlling and dandruff-removing composition includes the following steps: Preparation of oil-controlling and dandruff-removing composition: After stirring and mixing active ingredient A with polyol evenly, add active ingredient B and stir evenly. Finally, add the remaining water and stir and mix evenly. Set aside for later use. The oil-controlling and dandruff-removing compositions of Examples 1-4 were prepared according to the components of Examples 1-4 in Table 1.

[0045] Table 1. Composition of the oil-controlling and dandruff-removing compositions in Examples 1-4 (weight percentage, %) Among them, the bioflavonoids in Table 1 are obtained by separating the leaves of *Symplocos rubra*.

[0046] The oil-controlling and dandruff-removing compositions of Comparative Examples 1-5 were prepared according to the components of Comparative Examples 1-5 in Table 2.

[0047] Table 2. Composition of the oil-controlling and dandruff-removing compositions of Comparative Examples 1-5 (weight percentage, %) Among them, the bioflavonoids in Table 2 are obtained by separating them from the leaves of the spiny plant.

[0048] The oil-controlling and dandruff-removing compositions of Examples 1-4 and Comparative Examples 1-5 were applied to shampoo formulations to prepare shampoos of Examples 1-4 and Comparative Examples 1-5.

[0049] The preparation method of the shampoo includes the following steps: 1) After the polyquaternium-10 in phase A is pre-mixed and swollen evenly with water in the main pot, sodium lauryl ether sulfate is added, and the temperature is raised to 75℃-85℃. After it is completely dissolved by stirring, the remaining materials in phase A are added in sequence, and the temperature is maintained at 75℃ until it is completely dissolved. After dissolution, the temperature is lowered to 50℃ while stirring; 2) The phase B component is added to the main pot, the pH value is adjusted to 5.0-6.0, the stirring speed is controlled at 50 rpm, and the temperature is lowered after stirring for 30 minutes; 3) When the temperature drops to 45℃, the oil-controlling and dandruff-removing composition is added, the stirring is continued for 30 minutes, the temperature is further lowered, and the material is discharged at 35℃.

[0050] The composition of the shampoos in Experiments 1-4 is shown in Table 3 below: Table 3. Shampoo composition (weight percentage, %) for Test Examples 1-4 The composition of the shampoos in Comparative Examples 1-5 is shown in Table 4 below: Table 4. Shampoo composition (weight percentage, %) for Comparative Examples 1-5 The oil-controlling and dandruff-removing compositions of Examples 1-4 and Comparative Examples 1-5 were applied to scalp serum formulations to prepare scalp serums of Examples 5-8 and Comparative Examples 6-10.

[0051] The preparation method of scalp serum includes the following steps: 1) Add cold water to the main pot, add carbomer, let stand so that carbomer is completely immersed in cold water, stir at high speed first, then homogenize to make it completely swollen, and then heat to 65 to 75°C; 2) Add arginine, phenoxyethanol and p-hydroxyacetophenone, stir to make it completely dissolved, and then cool down; 3) When the temperature drops to 45°C, add oil-controlling and dandruff-removing composition, continue stirring for 30 minutes, continue cooling, and discharge at 35°C.

[0052] The composition of the scalp serums in Experiments 5-8 is shown in Table 5 below: Table 5. Composition of the scalp serums in Experiments 5-8 The composition of the scalp serums in comparative ratios 6-10 is shown in Table 6 below: Table 6. Composition of scalp serums in Comparative Examples 6-10 Performance testing: 1. Inhibitory effect test on Malassezia Referring to Part 7-7.5 of QB-T 2738-2012 "Evaluation Methods for Antibacterial and Bacteriostatic Effects of Daily Chemical Products" and Part 2.1.7.2 of the Ministry of Health's "Disinfection Technical Specifications" (2002 edition), the test strain selected was Mslassezia Furfur ATCC 44344.

[0053] The specific operation process is as follows: Take a Malassezia culture medium plate and punch a 5 mm diameter well. Inoculate with Malassezia furfur at a concentration of 5 × 10⁻⁶. 5 ~5×10 6 The test bacteria (cfu / mL) were evenly spread three times on a Malassezia agar plate and dried at room temperature. 20 μL of the test sample was dropped into a 5 mm diameter well of the Malassezia agar plate, the plate was covered, and the plate was incubated at 37 ℃. For the negative control, 20 μL of sterile distilled water was dropped into a 5 mm diameter well of the Malassezia agar plate, the plate was covered, and the plate was incubated at 37 ℃. The results were observed, the diameter of the inhibition zone was measured and recorded. The test was repeated three times, and the average value was taken. A inhibition zone diameter greater than 7 mm indicates an antibacterial effect; an inhibition zone diameter less than 7 mm indicates no antibacterial effect.

[0054] 2. Antibacterial activity test of Propionibacterium acnes The test strain selected was *Propionibacterium acnes* (ATCC 11827). Following the guidelines of QB-T2738-2012 "Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products" and QB-T 2850-2007 "Antibacterial and Bacteriostatic Detergents," the specific operating procedure is as follows: The test sample was diluted 1:5 with sterile standard hard water and mixed well; 0.1 mL was inoculated with Propionibacterium acnes at a concentration of 5 × 10⁻⁶. 5 ~5×10 6 Add the test bacteria (cfu / mL) to 5 mL of diluted test sample, mix well, and allow to contact for 2 min. Then, take 0.5 mL of the mixture of test bacteria and test sample and add it to 4.5 mL of buffer solution, mix thoroughly, and dilute appropriately. Then, take 0.5 mL of each of the 2-3 dilutions and place them in two petri dishes. Inoculate the petri dishes onto nutrient agar medium using the pour method. Incubate the prepared petri dishes at (35±2)℃ for 48 h, count the viable colonies, and record the results. Repeat the experiment 3 times and calculate the inhibition rate.

[0055] The formula for calculating the antibacterial rate is: Antibacterial rate (%) = (Ⅰ-Ⅱ) / I × 100%; In the formula: Ⅰ: average colony count of the control sample; Ⅱ: average colony count of the test sample.

[0056] The evaluation criteria for efficacy are: 1) Antibacterial rate ≥90%, significant antibacterial effect; 2) Antibacterial rate ≥50%-90%, antibacterial effect; 3) Antibacterial rate <50%, ineffective.

[0057] 3. Evaluation test of ability to inhibit biofilm formation / disintegrate biofilm To verify the inhibitory and destructive effects of the composition of this invention on the biofilms of Propionibacterium acnes and Malassezia, the ability to inhibit biofilm formation and destructive biofilms was determined using a 24-well plate crystal violet staining method. The specific procedures are as follows: 1) Test method for inhibiting biofilm formation: Add a certain amount of test sample solution to the blank wells of a 24-well plate, then add 2 mL of biofilm culture medium to each well. Mix well and place the plate in an anaerobic workstation for a certain period of time. Completely aspirate the suspension of the sample solution and culture medium mixture in the wells, place the plate in a 50℃ oven to dry and fix the biofilm for at least 4 hours, then add 2 mL of 0.1% crystal violet solution to each biofilm well, stain for 5 minutes, and then gently rinse the unbound crystal violet dye from each well with tap water. Observe the integrity and thickness of the biofilm at the bottom of each well, evaluate the biofilm in the sample solution wells, and evaluate its ability to inhibit biofilm formation. Note: Each test sample was tested at three concentrations: 10% (test concentration 1), 20% (test concentration 2), and 30% (test concentration 3).

[0058] 2) Test methods for the ability to disrupt biofilms: To assess the biofilm-disrupting ability of the composition described in this invention, a biofilm must first be cultured for a certain period. Then, the test sample is added to the biofilm wells and incubated for an additional time. The biofilm reduction rate is used to evaluate its biofilm-disrupting ability. The specific steps are as follows: 2 ml of biofilm culture solution is added to the blank wells of a 24-well plate, mixed well, and then the plate is placed in an anaerobic workstation for a certain period. The test sample solution is directly added to the biofilm wells and incubated for an additional time. The suspended sample solution and bacterial solution mixture in the wells is completely aspirated, and the plate is placed in a 50°C oven to dry and fix the biofilm for at least 4 hours. Then, 2 ml of 0.1% crystal violet solution is added to each biofilm well, and after staining for 5 minutes, the unbound crystal violet dye in each well is gently rinsed off with tap water. The integrity and thickness of the biofilm in each well at the bottom of the plate are observed to evaluate the biofilm in the sample solution wells and assess its biofilm-disrupting ability. Note: Each sample was tested at three concentrations: 10% (concentration 1), 20% (concentration 2), and 30% (concentration 3).

[0059] To evaluate the amount of biofilm in each well and the ability of the test sample to inhibit or break down the biofilm, this invention introduces four key indicators: relative integrity, relative thickness, biofilm fraction, and biofilm reduction rate. a. Relative Integrity (%): The biofilm area of ​​the simultaneously cultured, untreated control group is considered 100%. The percentage is calculated by visually comparing the area difference between the test wells and the control group. A higher value indicates a larger biofilm coverage area in the test well. b. Relative Thickness (%): The thickness of the test wells is measured and compared to the thickness of the simultaneously cultured, untreated control group (100%), and its percentage relative to the control group is calculated. A higher relative thickness value indicates a thicker biofilm. c. Biofilm Fraction: The value obtained by multiplying the relative integrity and relative thickness is used to quantify the three-dimensional volume of the biofilm in the test well. The biofilm fraction of the untreated control group is set to 10000 (=100×100). A higher biofilm fraction in the test well means a greater amount of biofilm adhering to the bottom of the well. d. Biofilm Reduction Rate (%): The Biofilm Reduction Rate (BRR) is defined as the ratio of the reduction in three-dimensional volume of biofilm in the test well relative to the control group. The calculation formula is: (Control biofilm fraction - Test biofilm fraction) / Control biofilm fraction × 100%. A higher biofilm reduction rate indicates a more significant effect of the sample solution in inhibiting or breaking down the biofilm in that well. Table 7 shows the evaluation criteria for the ability to inhibit biofilm formation / break down biofilm using the biofilm reduction rate as a parameter.

[0060] Table 7 Evaluation Criteria for Biofilm Inhibition / Biofilm Disintegration Capacity 4. Evaluation of oil control and dandruff removal efficacy This invention uses the Meibometer MB560 scalp oil analyzer to evaluate the efficacy of oil strips. Based on the principle of oil testing photometry, the lipid compounds (oil) in the scalp will change the transparency of the oil strip. The instrument's photoelectric device can measure the transmittance distribution curve on the oil strip; the peak value of the transmittance curve represents the content of lipid compounds, i.e., oil, in the scalp. A larger peak value indicates a higher oil content, and vice versa.

[0061] This invention uses the Cornemeter CM825 skin moisture test probe from CK Germany to test changes in scalp moisture content. The higher the measured value, the higher the moisture content of the scalp stratum corneum. It also uses the CK TMNano ultra-small skin moisture loss test probe from CK Germany to test scalp barrier function. The lower the measured value, the better the scalp barrier function.

[0062] Test samples: scalp serums from the experimental and comparative examples.

[0063] Test method: Forty-five healthy Chinese men and women aged 18-60 years with oily scalp and dandruff were selected, and their scalp oil content was measured to be ≥450 μg / cm³ by a scalp oil analyzer. 2 The participants were randomly divided into 5 groups for a half-head comparison test. Each participant used a scalp serum containing the experimental sample on the left side and a scalp serum containing the control sample on the right side. The participants' hair was parted in the middle, and they were randomly assigned to experimental and control groups 2cm to the left and right of the parting. Test sites were selected at 3cm, 4cm, and 5cm above the hairline. The sebum strip was left in contact with the scalp for 30 seconds, and the sebum content (peak value of the transmittance curve) at these three points was measured according to the instructions of the scalp sebum tester. The results were expressed as the average of three measurements. Similarly, the participants' hair was parted in the middle, and they were randomly assigned to experimental and control groups 2cm to the left and right of the parting. Test sites were selected at 3cm, 4cm, and 5cm above the hairline. The skin moisture content at these three points was measured according to the instructions of the Cornemeter CM825 skin moisture test probe, and the results were expressed as the average of three measurements. As above, the test subjects' hair was parted in the middle, and they were randomly divided into experimental and control groups 2cm to the left and right of the parting. Test sites were selected 3cm, 4cm, and 5cm above the hairline. Following the instructions for using the TMNano ultra-small skin moisture loss test probe, skin moisture loss was measured at these three points. The results were expressed as the average of the three measurements. Test subject usage requirements: the amount used should be the same on both sides each time, and it should be used once a day. Relevant indicators were evaluated before and 28 days after using the test sample.

[0064] Indicator Judgment: Oil control effect evaluation: Calculate the rate of change in scalp oil content of the test subject. The smaller the value, the stronger the ability of the tested product to reduce scalp oil content, that is, the better the oil control effect of the test sample. Rate of change in scalp oil content (%) = D 28 The value indicates the scalp oil content after 28 days of use, while D0 indicates the scalp oil content after 0 days of use.

[0065] Moisturizing effect evaluation: Calculate the rate of change in scalp moisture content of the test subject. The higher the rate of change, the better the moisturizing effect of the product. Rate of change in scalp moisture content (%) = D 28 The value indicates the scalp moisture content after 28 days of use, while D0 indicates the scalp moisture content after 0 days of use.

[0066] Evaluation of scalp barrier improvement: The change rate of transepidermal water loss from the scalp was calculated. A lower value indicates improved scalp barrier function. Change rate of transepidermal water loss from the scalp (%) = D28 D0 represents the rate of scalp transepidermal water loss after 28 days of use, while D0 represents the rate of scalp transepidermal water loss after 0 days of use.

[0067] Dandruff Reduction Effect Evaluation: After 28 days of using the test sample, professionals assessed the test subject's dandruff condition and scored it to determine the dandruff reduction effect of the test sample. A scoring system was used, with the following criteria: 5 points represent large, visible flakes of dandruff; 4 points represent a relatively large amount of fine dandruff; 3 points represent a relatively small amount of fine dandruff; 2 points represent no detectable dandruff by the test subject, but a very small amount still detected by the professional; and 1 point represents no dandruff at all. The reduction rate of dandruff score before and after using the test sample was compared; a higher reduction rate indicates a better dandruff reduction effect. Dandruff score reduction rate (%) = D 28 The value represents the dandruff score after 28 days of use, while D0 represents the dandruff score after 0 days of use.

[0068] The shampoos from Experimental Examples 1-4 and Comparative Examples 1-5 were tested for their inhibitory effect on Malassezia. The test results are shown in Table 8 below: Table 8. Results of the inhibitory effect of shampoos containing Malassezia in Experimental Examples 1-4 and Comparative Examples 1-5 According to the test results in Table 8, the antibacterial effect of Experiment 1 is significantly better than that of Comparative Example 1, indicating that the lack of active ingredient A greatly reduces the inhibitory effect on Malassezia growth, further demonstrating that there is a significant synergistic effect between active ingredient A and active ingredient B; the antibacterial effect of Experiment 2 is significantly better than that of Comparative Example 2, indicating that without active ingredient B to inhibit the growth of Malassezia, the microorganism will continue to grow, making it difficult to effectively inhibit the growth of Malassezia, further demonstrating that there is a significant synergistic effect between active ingredient A and active ingredient B, and neither can be omitted.

[0069] The antibacterial effect of Example 3 was significantly better than that of Comparative Examples 3 and 4, indicating that if the concentration of active ingredient A is too low or the weight ratio of active ingredient A to active ingredient B is outside the range defined by this invention, it cannot effectively exert a synergistic effect and therefore cannot inhibit the excessive proliferation of Malassezia. This proves that active ingredient A needs to reach a certain concentration to produce a synergistic effect with active ingredient B. In addition, the test results of Comparative Example 4 also show that when the ratio of active ingredient A to active ingredient B is too high, that is, the concentration of active ingredient A is too high and the concentration of active ingredient B is relatively insufficient, the low concentration of active ingredient B cannot effectively inhibit Malassezia, resulting in the excessive survival of a large number of Malassezia. This proves that active ingredient B also needs a sufficient concentration to ensure the effect of inhibiting Malassezia. When the optimal ratio is deviated from, the synergistic effect of active ingredients A and active ingredient B disappears.

[0070] The antibacterial effect of Example 4 was significantly better than that of Comparative Example 5, indicating that the ratio of active ingredient A to active ingredient B was within the range of the invention. However, it did not have the effect of inhibiting Malassezia, indicating that the lack of the penetration-promoting and dissolving effect of polyols led to a decrease in the activity of active ingredients A and B when acting on Malassezia, and thus could not effectively inhibit the growth of Malassezia.

[0071] In summary, active ingredient A, active ingredient B, and polyol are all indispensable, together forming a complete system that synergistically inhibits the growth of Malassezia, ultimately achieving a better dandruff removal effect.

[0072] The shampoos from Experimental Examples 1-4 and Comparative Examples 1-5 were tested for their antibacterial activity against Propionibacterium acnes. The test results are shown in Table 9 below: Table 9. Results of the inhibitory effect of shampoos containing Propionibacterium acnes in Experimental Examples 1-4 and Comparative Examples 1-5. The proliferation of Propionibacterium acnes is a key factor in the development of acne (pimples). When there is excessive sebum secretion or abnormal keratinization of the hair follicle opening, Propionibacterium acnes proliferates rapidly. Furthermore, Propionibacterium acnes breaks down triglycerides in sebum, releasing free fatty acids and other substances that trigger inflammatory responses. Simultaneously, the excessive proliferation of Propionibacterium acnes disrupts the balance of the scalp's surface flora, leading to a decline in the scalp barrier function. Therefore, effectively inhibiting the growth of Propionibacterium acnes can reduce inflammatory responses and improve scalp barrier function.

[0073] According to the test results in Table 9, Example 1 showed excellent inhibitory performance against Propionibacterium acnes. However, Comparative Example 1, which lacked active ingredient A, had no inhibitory effect on Propionibacterium acnes, proving that the absence of active ingredient A prevents the achievement of excellent inhibitory effects against Propionibacterium acnes. Example 2 showed significantly better inhibitory effects against Propionibacterium acnes than Comparative Example 2, indicating that active ingredient A alone has a certain antibacterial effect on Propionibacterium acnes. However, the effect is far less than that of Example 2, which contains both active ingredients A and B, proving that the absence of active ingredient B prevents excellent inhibition of Propionibacterium acnes. The antibacterial effect of Example 3 was significantly better than that of Comparative Examples 3 and 4. In Comparative Example 3, the concentration of active ingredient A was too low to achieve an effective synergistic concentration. In Comparative Example 4, the ratio of active ingredient A to active ingredient B was too high. Although the concentrations of active ingredients A and B were sufficient, they could not achieve an effective synergistic concentration, resulting in a decreased inhibitory effect on Propionibacterium acnes and failing to achieve a significant antibacterial effect. The comparative data of Example 3 with Comparative Examples 3 and 4 further proves that active ingredients A and B must meet the ratio range specified in this invention to exert a synergistic antibacterial effect. The superior inhibitory effect of Example 4 on Propionibacterium acnes compared to Comparative Example 5 indicates that when the ratio of active ingredient A to active ingredient B is within the range required by this invention, the lack of the penetration-enhancing and dissolving effect of polyols leads to a decrease in the activity of active ingredients A and B when acting on Propionibacterium acnes, failing to achieve significant inhibition of Propionibacterium acnes growth. This proves that polyols are key functional components; their absence results in a sharp decline in the overall antibacterial performance of the system. In summary, active ingredient A, active ingredient B, and polyol are all indispensable and together constitute an inhibitory system against Propionibacterium acnes; at the same time, the ratio of active ingredient A to active ingredient B must meet the requirements of this invention in order to achieve an effective inhibitory effect on Propionibacterium acnes.

[0074] The scalp serums from Examples 5-8 and Comparative Examples 6-10 were tested for their ability to inhibit biofilm formation. The test results are shown in Table 10 below. Table 10 Evaluation results of the ability of scalp serums in Experiments 5-8 and Comparative Examples 6-10 to inhibit biofilm formation. The scalp serums from Experimental Examples 5-8 and Comparative Examples 6-10 were subjected to a biomembrane disruption ability test. The test results are shown in Table 11 below. Table 11. Evaluation results of the biofilm-disrupting ability of the scalp serums in Examples 5-8 and Comparative Examples 6-10. Figure 1 This is a test diagram showing the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to inhibit the formation of Malassezia biofilm. Among them, Figure 1M1 is the scalp serum of Experimental Example 5, M2 is the scalp serum of Experimental Example 6, M3 is the scalp serum of Experimental Example 7, M4 is the scalp serum of Experimental Example 8, M5 is the scalp serum of Comparative Example 6, M6 is the scalp serum of Comparative Example 7, M7 is the scalp serum of Comparative Example 8, M8 is the scalp serum of Comparative Example 9, and M9 is the scalp serum of Comparative Example 10.

[0075] Figure 2 This is a graph showing the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of the present invention to inhibit the formation of Propionibacterium acnes biofilm. Among them, Figure 2 In the table, P1 is the scalp serum of Test Example 5, P2 is the scalp serum of Test Example 6, P3 is the scalp serum of Test Example 7, P4 is the scalp serum of Test Example 8, P5 is the scalp serum of Comparative Example 6, P6 is the scalp serum of Comparative Example 7, P7 is the scalp serum of Comparative Example 8, P8 is the scalp serum of Comparative Example 9, and P9 is the scalp serum of Comparative Example 10.

[0076] Figure 3 This is a graph showing the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to disrupt the Malassezia biofilm. Among them, Figure 3 M1 is the scalp serum of Experimental Example 5, M2 is the scalp serum of Experimental Example 6, M3 is the scalp serum of Experimental Example 7, M4 is the scalp serum of Experimental Example 8, M5 is the scalp serum of Comparative Example 6, M6 is the scalp serum of Comparative Example 7, M7 is the scalp serum of Comparative Example 8, M8 is the scalp serum of Comparative Example 9, and M9 is the scalp serum of Comparative Example 10.

[0077] Figure 4 This is a graph showing the ability of the scalp serum formulations of Experimental Examples 5-8 and Comparative Examples 6-10 of this invention to disrupt the biofilm of Propionibacterium acnes. Figure 4 In the table, P1 is the scalp serum of Test Example 5, P2 is the scalp serum of Test Example 6, P3 is the scalp serum of Test Example 7, P4 is the scalp serum of Test Example 8, P5 is the scalp serum of Comparative Example 6, P6 is the scalp serum of Comparative Example 7, P7 is the scalp serum of Comparative Example 8, P8 is the scalp serum of Comparative Example 9, and P9 is the scalp serum of Comparative Example 10.

[0078] The test results in Tables 10 and 11 show that the scalp serums in Examples 5-8 all achieved a Level I rating in their ability to inhibit the formation of Malassezia and Propionibacterium acnes biofilms, and a Level I or II rating in their ability to break down these biofilms. This demonstrates their strong inhibitory and scavenging abilities against both planktonic bacteria and biofilms. This reflects the perfect synergistic effect of active ingredient A, active ingredient B, and the polyol: the polyol promotes penetration, active ingredient B effectively attacks the biofilm matrix, and active ingredients A and B work together to efficiently kill the internal flora and regulate the microenvironment to prevent recurrence after the biofilm is destroyed. Comparative Example 6 showed some ability to inhibit / break down Malassezia biofilms, but almost no ability to inhibit / break down Propionibacterium acnes biofilms. This indicates that although active ingredient B has some effect on Malassezia, the lack of active ingredient A prevents the complete inhibition and scavenging of the bacterial biofilm, resulting in incomplete efficacy. Comparative Example 7 was ineffective in inhibiting / breaking down either Malassezia or Propionibacterium acnes biofilms. This demonstrates that active ingredient B is the core of the anti-biofilm system; without it, the system loses its ability to inhibit and break down biofilms. Compared to Experiment 7 and Comparative Examples 8 and 9, Comparative Example 8 had too low an active ingredient A content, and Comparative Example 9 had too high an active ingredient A:active ingredient B ratio. Neither resulted in the inhibition or removal of bacterial biofilms. This proves that when the concentration of active ingredient A or active ingredient B does not meet the requirements of this invention, although there is some ability to inhibit the formation of planktonic bacteria, the ability to inhibit biofilm formation and removal cannot be achieved. This further proves that active ingredients A and B must meet the ratio requirements specified in this invention to truly exert the best synergistic effect. Although Comparative Example 10 contained both active ingredients A and B, its ability to inhibit and break down biofilms was drastically lost, and it even showed a tendency to promote biofilm formation. This directly proves that the permeation-enhancing and dissolving effects of polyols are crucial; without them, the active ingredients cannot effectively penetrate deep into the biofilm to exert their effects, leading to the failure of the synergistic system. The above results further demonstrate that the active ingredient A, active ingredient B, and polyol in the oil-controlling and dandruff-removing composition of this invention are all indispensable, forming a synergistic system that can effectively inhibit and disintegrate the Malassezia / Propionibacterium acnes biofilm. Simultaneously, the weight ratio of active ingredient A to active ingredient B must be strictly controlled between (0.05-1):5. Only within this ratio range can the two produce the strongest synergistic effect, achieving highly efficient inhibition and disintegration of the biofilm. The absence or improper proportion of any component will only result in a suboptimal, incomplete antibacterial effect, rather than a superior effect in inhibiting and disintegrating the biofilm.

[0079] The scalp serums from Experiments 5-8 and Comparative Examples 6-10 were tested to verify and evaluate their oil-control and dandruff-reducing effects. The test results are shown in Tables 12-13 below: Table 12. Evaluation results of the oil control and dandruff removal efficacy tests of the scalp serums in Experiments 5-8 and Comparative Examples 6-10. Table 13. Evaluation results of the oil control and dandruff removal efficacy tests of the scalp serums in Experiments 5-8 and Comparative Examples 6-10. As shown in Tables 12-13, the scalp oil content in Experiments 5-8 was significantly reduced, with the absolute value of the change rate exceeding 50%, while the moisture content significantly increased. The transepidermal water loss rate was also significantly reduced, with the absolute value of the change rate exceeding 30%. This indicates that the oil-controlling and dandruff-reducing composition of the present invention added to the scalp serums of Experiments 5-8 can improve scalp barrier function, regulate sebum secretion, and increase scalp moisture content, thereby achieving a water-oil balance in the scalp and fundamentally controlling oil production. Furthermore, compared to the comparative example, the absolute value of the dandruff score reduction after using the scalp serum formulas from the experimental examples was higher than 75%, achieving excellent dandruff-reducing effects. This demonstrates that, under the specified ratio requirements of this invention, active ingredients A and B, along with the polyol, produce a highly efficient synergistic effect. Firstly, oil control and dandruff removal work synergistically: active ingredient B inhibits and removes biofilms, creating a healthy microenvironment for active ingredient A to regulate sebaceous glands; active ingredient A regulates sebum secretion levels, reducing the nutrient source for Malassezia and enhancing the dandruff-removing durability of active ingredient B. Secondly, barrier repair and moisturizing effects work synergistically: active ingredient A and the polyol synergistically improve the scalp barrier, reduce transepidermal water loss, and enhance moisturizing effects; simultaneously, the polyol system ensures efficient penetration and dissolution of active ingredients A and B; ultimately achieving reduced sebum restriction, increased scalp moisture content, enhanced barrier function, and effective dandruff removal. Comparative Example 6 lacks active ingredient A. Although active ingredients B and the polyol provide some dandruff removal and moisturizing effects, due to the absence of active ingredient A, the changes in scalp oil content (-4.7%) and transepidermal water loss (-9.8%) are relatively small, indicating extremely limited oil control and barrier repair effects. The absence of active ingredient A indicates that the product cannot achieve source oil control and effective barrier repair, resulting in incomplete efficacy. Comparative Example 7 lacks active ingredient B. Although active ingredients A and polyols provide some oil control (scalp oil content change rate (-6.6%)) and barrier improvement (transepidermal water loss rate change rate -13.7%), the lack of active ingredient B prevents effective combating of Malassezia and its biofilm, leading to poor dandruff removal and failing to achieve effective dandruff removal. Comparative Example 8 has an excessively low content of active ingredient A, resulting in negligible oil control (scalp oil content change rate -5.1%) and barrier improvement (transepidermal water loss rate change rate -1.8%). Although active ingredient B alone has some dandruff-removing effect, its overall performance is far inferior to that of Example 7. The ratio of active ingredient A to active ingredient B in Comparative Example 9 was too high, resulting in a very poor dandruff-reducing effect (dandruff score reduction rate of -7.1%). Although active ingredient A alone has a certain oil-controlling effect, the overall effect is far inferior to that of Example 7. This indicates that active ingredients A and B must meet the ratio range required by this invention in order to produce a comprehensive synergistic effect, rather than a single effect.The ratio of active ingredient A and active ingredient B in Comparative Example 10 was within the range required by this invention, but it lacked polyol. The experimental results showed that all efficacy indicators dropped sharply, and the moisturizing performance (moisture content change rate of 4%) and barrier function (transepidermal water loss rate change rate of -5.9%) were almost lost. This indicates that polyol is not only a solvent, but also a key component for achieving moisturizing, penetration promotion, and synergistic removal of biofilms. Without it, the entire synergistic system fails.

[0080] In summary, the oil-controlling and dandruff-removing composition of the present invention surpasses traditional single-function products (simple antibacterial, simple dandruff removal, or simple oil control). It innovatively integrates four major functions: oil control, antibacterial and biofilm removal, dandruff removal, and penetration promotion. It achieves synergistic regulation of scalp microecology, sebum levels, and scalp barrier, successfully solving the problems of short-lasting oil control, easy recurrence of dandruff removal, and easy damage to the scalp barrier in the prior art.

[0081] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and these modifications or substitutions should all be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. An oil control anti-dandruff composition characterized in that, comprising a polyol, an active ingredient A and an active ingredient B; said active ingredient A is selected from the group consisting of bioflavonoids and / or plankton extract; said active ingredient B is selected from at least one of zinc gluconate, zinc lactate, zinc PCA, zinc coceth sulfate, zinc hyaluronate.

2. The oil control antidandruff composition according to claim 1, wherein consisting of a polyol, an active ingredient A, an active ingredient B and a balance of water.

3. The oil control and anti-dandruff composition according to claim 1, wherein consisting of a polyol 20-55%, an active ingredient A 0.2-6%, an active ingredient B 2-20% and a balance of water by weight percentage.

4. The oil control and anti-dandruff composition according to claim 1, wherein said bioflavonoids are isolated from the leaves of Gleditsia japonica.

5. The oil control and anti-dandruff composition according to claim 1, wherein said polyol is selected from at least one of 1,3-propanediol, 1,2-propanediol, 1,2-butanediol, glycerol, pentanediol, hexanediol.

6. The oil control and anti-dandruff composition according to claim 1, wherein consisting of a polyol 25-50%, an active ingredient A 0.5-5%, an active ingredient B 3-15% and a balance of water by weight percentage.

7. The oil control and dandruff treatment composition according to Claim 1, wherein said active ingredient A and active ingredient B are in a weight ratio of (0.05-1):

5.

8. The oil control and dandruff treatment composition according to Claim 1, wherein said active ingredient A and active ingredient B are in a weight ratio of (0.1-1):

5.

9. Use of the oil-control and dandruff-removing composition according to any one of claims 1-8 in a hair care product.

10. Use of the oil control antidandruff composition according to claim 9 in a hair care product, characterized in that, said oil-control and dandruff-removing composition accounts for 0.2-10% of the hair care product by weight percentage.

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