Application of salmon nasal cartilage extract in preparation of product for improving bone mineral density

By extracting and purifying salmon nasal cartilage, a salmon nasal cartilage extract that can promote osteocyte proliferation was prepared, overcoming the shortcomings of existing technologies in improving bone density and achieving the effect of improving bone density.

CN121428049APending Publication Date: 2026-01-30NATAI (YANGJIANG) BIOTECHNOLOGY CO LTD
View PDF 5 Cites 0 Cited by

Patent Information

Application Number
CN202511606484.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-05
Publication Date
2026-01-30

AI Technical Summary

Technical Problem

Existing research has not reported that salmon cartilage extract can effectively increase bone density, and the mechanism by which it increases bone density is lacking.

Method used

Extracts were obtained from salmon nasal cartilage through steps such as guanidine hydrochloride extraction, acetone fractionation precipitation, enzymatic hydrolysis, ion chromatography, and ultrafiltration. The extracts were then purified by hydroxyapatite ion chromatography and the components with a molecular weight of less than 3 kDa were screened to prepare an extract that promotes the proliferation of bone embryonic cells.

Benefits of technology

This extract can effectively enhance the proliferation capacity of bone cells and improve bone density, making it suitable for postoperative recovery from osteoporosis, osteomalacia, and orthopedic diseases.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure SMS_1
    Figure SMS_1
  • Figure SMS_2
    Figure SMS_2
Patent Text Reader

Abstract

The invention belongs to the technical field of cartilage extracts, and particularly relates to application of a salmon nasal cartilage extract in preparation of a product for improving bone mineral density. The preparation method of the extract comprises the following steps: 1) performing low-temperature extraction on salmon nasal cartilage homogenate by using guanidine hydrochloride, centrifuging, and dialyzing to intercept total protein with the molecular weight of more than 8000; 2) adding precooled acetone into the total protein for fractional precipitation, and freeze-drying to obtain fractional precipitation protein; 3) dissolving the fractional precipitation protein in diluted hydrochloric acid, adding trypsin, and carrying out light-shielding enzymolysis to obtain an enzymatic hydrolysate; and (4) carrying out hydroxyapatite ion chromatography on the enzymatic hydrolysate, and carrying out ultrafiltration to intercept components with the molecular weight of less than 3kDa, so as to obtain the salmon nasal cartilage extract. The extract can significantly improve the multiplication capacity of embryo osteogenesis cell strains, can improve the bone mineral density value of shin bones of SD rats, has wide application prospects in the fields of food, health care products and medicines, and is especially suitable for development of bone health related products.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Preparation method of raja porosa chondroprotein anti-oxidation peptide

    CN105420330A

  • Dasyatis akajei chondroprotein antioxidative peptide and application thereof

    CN105646651A

  • Preparation method of non-denatured II-type collagen for increasing bone mineral density and improving bone elasticity

    CN120463799A

  • High-activity calcium chelating peptide derived from cod bone and preparation method of high-activity calcium chelating peptide

    CN120623315A

  • Bone morphogenetic protein composition

    US4789732A