Library building kit and probe for detecting blood tumor related fusion genes

Abstract

The invention belongs to the technical field of gene sequencing, and particularly relates to a library building kit and probe for detecting blood tumor related fusion genes. According to the library building kit, only a trace amount of total RNA is needed to be used for cDNA synthesis, the initial quantity of a template can be as low as 10 ng, so that a cDNA library is built, then the library is subjected to hybrid capture and enrichment by using an autonomously designed probe hematologic tumor fusion gene Panel (RA Panel), the time consumption is short, the efficiency is high, and simultaneous building and on-machine sequencing of 384 libraries can be realized. According to the rapid hybridization system of the library building kit, the shortest hybridization time can reach 30 min, the size of a finally obtained library fragment is about 200-400 bp, and the rapid hybridization system can be compatible with various Illumina sequencing platforms for sequencing. According to the invention, through a simple and easy-to-use solution of NGS sequencing while synthesis, rapid and accurate typing of fusion genes which may affect blood tumor diagnosis is realized.

Description

Technical Field

[0001] This invention belongs to the field of gene sequencing technology, specifically relating to a library preparation kit and probe for detecting fusion genes related to hematologic malignancies using a hybridization capture method. Background Technology

[0002] Fusion genes are a common molecular biological abnormality in hematologic malignancies, usually caused by chromosomal structural rearrangements (such as chromosomal insertions, deletions, translocations, or inversions). Although the functional effects of many gene fusions remain to be studied, the vast majority of gene fusions ultimately lead to tumorigenesis. Because recurrent gene fusions are significantly associated with different tumor types, gene fusion testing has been recommended for screening common tumor types. The well-known BCR-ABL1 fusion gene on the Philadelphia chromosome is a typical fusion oncogene, closely associated with chronic myeloid leukemia (CML). Currently, this gene is used as a diagnostic marker and to monitor patient response to treatment. Due to the heterogeneity of gene fusion states in some morphologically homogeneous malignant tumors, they play an important role in treatment stratification, such as the different mixed lineage leukemia gene (MLL) fusion types in acute myeloid leukemia (AML). Therefore, fusion genes can be used as a basis for molecular subtyping of hematological malignancies, which is of great significance for prognostic judgment and guiding the selection of treatment plans. They can also be used as a highly sensitive indicator for monitoring minimal residual disease (MRD). Accurate detection and analysis of fusion genes are of great significance for the diagnosis, subtyping, prognostic assessment and targeted therapy of hematological diseases.

[0003] With the development of molecular biology techniques, methods for detecting fusion genes have continuously improved. Traditional methods, such as cytogenetic karyotype analysis and fluorescence in situ hybridization (FISH), can detect large chromosomal abnormalities, but their resolution is limited, potentially missing occult gene rearrangements, and are limited by the number of genes screened. In recent years, the maturity and widespread application of high-throughput gene sequencing technology have significantly improved the detection rate and accuracy of fusion genes, while also enabling the discovery of unknown fusion partner genes, providing a powerful tool for sequencing and identifying fusion transcripts. Compared to traditional genomic DNA detection, mRNA-based fusion gene detection is widely used clinically due to its higher sensitivity and accuracy, as well as its higher analytical efficiency. mRNA detection can directly capture fusion transcripts (mRNA), avoiding missed detections caused by complex intron structures or breakpoints located in large introns. This is crucial for clinical screening and minimal residual disease (MRD) monitoring, enabling earlier detection of low-level residual disease and warning of relapse risk. It can also directly confirm whether fusion transcripts are successfully expressed and identify different isoforms produced by alternative splicing. For complex translocations involving multiple genes, mRNA detection can more clearly reveal the final fusion pattern. This has clear guiding significance for assessing the pathogenicity of fusion genes and subsequent targeted therapy selection. At the same time, because the target region (exon region) is relatively concentrated, mRNA sequencing usually requires less data, is simpler to analyze, and can sometimes achieve faster reporting cycles, saving valuable time for clinical decision-making.

[0004] To refine the detection of gene fusions and improve accuracy, this invention selects 26 pairs of the most authoritative fusion genes from NCCN guidelines, WHO classification of hematologic malignancies, and related literature. Specific probes are designed and packaged into a kit for detecting gene fusions in patients. Compared to other kits, this invention's kit offers high specificity, low cost, and the ability to detect gene fusions at a lower frequency. Summary of the Invention

[0005] The present invention aims to overcome the shortcomings of low sensitivity, low specificity and low accuracy of existing gene detection technologies, and provides a library preparation kit and probe for detecting fusion genes related to hematological malignancies.

[0006] To achieve the above-mentioned objectives, the present invention is implemented through the following technical solution: A probe set for detecting hematologic malignancy-related fusion genes, the probe set comprising a plurality of probes, the base sequences of the probes being selected from at least one of SEQ ID NO. 1 to 246.

[0007] Preferably, the probe coverage region contains a hematologic malignancy-related fusion gene, which is selected from at least one of the following groups: RBM15::MRTFA, GATA2::MECOM, NPM1::MLF1, MYC::BCL6, FIP1L1::PDGFRA, KMT2A::AFF1, NUP98 ::NSD1, PDGFRB::ETV6, BCL11B::TLX3, DUSP22::IRF4, KMT2A::AFDN, NUP98::HOXA13, MNX1::ET V6, PCM1::JAK2, MYC::BCL2, RUNX1::RUNX1T1, NUP214::ABL1, KMT2A::MLLT3, BCR::ABL1, KMT2A ::MLLT10, BIRC3::MALT1, KMT2A::ELL, KMT2A::MLLT1, ETV6::RUNX1, PML::RARA, CBFB::MYH11.

[0008] The application of the probe set for detecting hematologic malignancy-related fusion genes as described above in the preparation of a library preparation kit for detecting hematologic malignancy-related fusion genes by hybridization capture method.

[0009] A library preparation kit for detecting hematologic malignancy-related fusion genes, the library preparation kit comprising the probe set described above.

[0010] This invention provides a library construction kit for detecting hematologic malignancy-related fusion genes using a hybridization capture method. Using a very low starting amount of total RNA as a template, the RNA is fragmented, reverse transcribed into two-stranded cDNA, and end-repaired with an A-tail. An adapter is then added, followed by magnetic bead purification. After a brief index amplification, the library is constructed. A personalized probe hematologic malignancy fusion gene panel (RA Panel) is then used for rapid hybridization, achieving hybridization efficiency that typically requires overnight reactions within 30 minutes. The hybridization products are then captured, enriched, amplified, and purified using streptomycin magnetic beads to obtain the final targeted library. High-throughput sequencing is then performed on the targeted library. After sequencing data is obtained, bioinformatics analysis and comparison are performed using relevant bioinformatics analysis software and pharmacogenomics databases to rapidly detect hematologic malignancy-related fusion genes.

[0011] The library preparation kit for detecting hematologic malignancy-related fusion genes using the hybridization capture method of the present invention can be used to assist in the diagnosis and classification of hematologic malignancy patients, guide treatment selection, assess prognosis and monitor hematologic malignancy patients.

[0012] Preferably, the library preparation kit further includes reagents required for hybridization capture, wherein the reagents are selected from at least one of hybridization buffer, streptomycin magnetic beads, and index primers.

[0013] A method for using a library preparation kit for detecting fusion genes related to hematologic malignancies includes the following steps: Biological samples were provided, hybridization was performed using the probe set described above, and the fusion gene was detected by sequencing analysis.

[0014] Preferably, the biological sample is an RNA sample.

[0015] Preferably, the step of constructing a cDNA library from an RNA sample is also included.

[0016] As a preferred method, the process of constructing a cDNA library from an RNA sample specifically includes RNA fragmentation, reverse transcription, and end repair.

[0017] Preferably, the initial amount of the RNA sample is 10-200 ng.

[0018] Therefore, the present invention has the following beneficial effects: (1) The library preparation kit of the present invention can detect hematologic tumor-related fusion genes by using hybridization capture method. Only a small amount of total RNA is needed for cDNA synthesis. The template starting amount can be as low as 10 ng to construct a cDNA library. Then, the library is hybridized and enriched by using the self-designed probe hematologic tumor fusion gene Panel (RA Panel). It is time-saving and efficient. (2) The library construction kit of the present invention has up to 384 index pairs, which can realize the simultaneous construction and sequencing of 384 libraries; (3) The rapid hybridization system of the library preparation kit of the present invention can achieve the efficiency of general overnight hybridization in as little as 30 min, and finally obtain a library fragment size of about 200~400bp, which can be compatible with various Illumina sequencing platforms for sequencing. (4) This invention enables rapid and accurate typing of fusion genes that may affect the diagnosis of hematological malignancies through a simple and easy-to-use synthesis-on-NGS sequencing solution; (5) The library preparation kit of the present invention is simple to operate, takes little time, and is very easy to automate. It helps doctors predict patients’ responses to chemotherapy drugs, thereby developing individualized medication plans to reduce toxic side effects, improve efficacy, and prolong patients’ lifespan. At the same time, it can also assess patients’ prognosis and monitor their prognosis. It has good clinical application prospects and is easy to promote and apply in clinical practice. Attached Figure Description

[0019] Figure 1 This is a graph showing the fragment analysis results of the cDNA library constructed in Example 1. Detailed Implementation

[0020] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. Those skilled in the art will be able to implement the present invention based on these descriptions. Furthermore, the embodiments of the present invention described below are generally only some, not all, of the embodiments of the present invention. Therefore, all other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort should fall within the scope of protection of the present invention.

[0021] Example 1: [Document Library Construction] Step 1: cDNA Synthesis RNA sample requirements: Total sample size: 10-200 ng; quantification is preferably performed using a nucleic acid fluorescence quantitative PCR instrument. Sample purity: OD260 / OD280 = 1.8~2.0; OD260 / OD230 = 2.0~2.5; Sample quality: RNA should be quality checked before the experiment and graded according to RIN.

[0022] Step 2: RNA fragmentation & random primer binding 1. Take out the 1st Strand Synth Buffer and Random Primer and place them on ice to thaw naturally. Mix well and centrifuge briefly for later use. 2. Prepare the reaction system in a 0.2 mL PCR tube placed on ice, according to Table 1 below: Table 1: Reagent Single reaction volume 1st Strand Synth. Buffer 4 μL Random Primer 1 μL RNA 5 μL Total 10 μL

[0023] 3. Mix thoroughly and centrifuge briefly to place all reaction solution at the bottom of the PCR tube; 4. Place the PCR tube into the PCR instrument and perform fragmentation according to the settings in Table 2 below: Table 2: Temperature Time 94℃ 8 min

[0024] Step 3: First-strand synthesis of cDNA 1. Take out the 1st Strand Synth. Enzyme and place it on ice to thaw naturally. Mix well and centrifuge briefly for later use. 2. Prepare the reaction system in a 0.2 mL PCR tube placed on ice, according to Table 3 below: Table 3: Reagent Single reaction volume Fragmented RNA & Random Primer Binding Product 10 μL 1st Strand Synth. Enzyme 2 μL Nuclease Free Water 8 μL Total 20 μL

[0025] 3. Mix thoroughly and centrifuge briefly to place all reaction solution at the bottom of the PCR tube; 4. Place the PCR tubes into the PCR instrument and start the following program according to Table 4: Table 4: Temperature Time 25℃ 10 min 42℃ 15 min 70℃ 15 min 4℃ Hold

[0026] 5. Return any unused reagents to the container and store at -25 to -15°C.

[0027] Step 4: cDNA Two-Strand Synthesis 1. Take out the 2nd Strand Synth. Buffer and 2nd Strand Synth. Enzyme (Naonda) and place them on ice to thaw naturally. Mix well and centrifuge briefly for later use. 2. Prepare the reaction system in a 0.2 mL PCR tube placed on ice, according to Table 5 below: Table 5: Reagent Single reaction volume 1st Strand Synthesis Product 20 μL 2nd Strand Synth. Buffer 27 μL 2nd Strand Synth. Enzyme 3 μL Total 50 μL

[0028] 3. Mix thoroughly and centrifuge briefly to place all reaction solution at the bottom of the PCR tube; 4. Start the following reaction program on the PCR instrument according to Table 6 below, and place the reaction tube into the PCR instrument when the temperature stabilizes at 16℃; Table 6: Temperature Time 16℃ 60 min 4℃ Hold

[0029] Note: No heated lid is required during this step.

[0030] Step 5: Purification of Double-Stranded DNA 1. Add 90 μL of NadPrep® SP Beads to the cDNA double-strand synthesis product from step 3, mix well, and incubate at 25°C for 10 min; 2. After briefly centrifuging the PCR tube, place it on a magnetic rack for 5 minutes until the liquid is completely clear. Use a pipette to remove and discard the supernatant. Note: It must be fully clarified that the placement time may need to be adjusted for different brands of magnetic racks; 3. Slowly add 150 μL of 80% ethanol along the side wall of the PCR tube, being careful not to disturb the magnetic beads. Let it stand for 30 seconds, then use a pipette to remove and discard the supernatant. 4. Repeat step 3 once; 5. After briefly centrifuging the PCR tube, place it on a magnetic rack and use a 10 μL pipette tip to remove a small amount of residual ethanol, being careful not to pick up the magnetic beads. 6. Open the PCR tube cap and let it stand at room temperature for about 5 minutes until the ethanol has completely evaporated; Note: Do not over-dry, otherwise the yield will be reduced; 7. Remove the PCR tube, add 45 μL of Nuclease Free Water to the PCR tube, use a pipette to suspend the magnetic beads evenly, and incubate at 25°C for 2 min. 8. After briefly centrifuging the PCR tube, place it on a magnetic rack for 2 minutes until the liquid is completely clear. Carefully transfer the supernatant to a new 0.2 mL PCR tube using a pipette, being careful not to aspirate the magnetic beads. 9. Use a nucleic acid fluorescence quantitative analyzer to quantify double-stranded DNA; 10. Double-stranded DNA products should avoid repeated freeze-thaw cycles. They can be stored at -25~-15℃ for one week or at -80℃ for longer-term storage.

[0031] Step Six: Constructing a cDNA Library 1. End repair and A-tail addition First, turn on the PCR instrument and set the temperature of the PCR instrument's hot cover to 70℃; (1) Take out End Repair & A-Tailing Buffer Pro (Naonda) and melt it at room temperature. Mix it well and place it on ice for later use. (2) Take out End Repair & A-Tailing Enzyme Pro and place it on ice to melt naturally. Mix well and centrifuge briefly for later use. (3) Prepare the reaction system in a 0.2 mL PCR tube placed on ice, according to Table 7 below: Table 7: Reagent Single reaction volume End Repair & A-Tailing Buffer Pro 6 μL End Repair & A-Tailing Enzyme Pro 4 μL cDNA 40 μL Total 50 μL

[0032] (4) After mixing thoroughly, centrifuge briefly to place all the reaction solution at the bottom of the PCR tube; (5) According to Table 8 below, start the corresponding reaction program on the PCR instrument according to the sample type. When the temperature stabilizes at 25℃, put the reaction tube into the PCR instrument and set the heat cap to 70℃: Table 8: Temperature Time 25℃ 20 min 65℃ 20 min 10℃ HOLD

[0033] 2. Connector connection Take out the Ligation Buffer (Naonda) and melt it at room temperature. Mix it well and place it on ice for later use. (1) Take out the DNA Ligase (Naonda) and place it on ice to thaw naturally. Mix well and centrifuge briefly for later use. (2) Remove the PCR reaction tubes from step two from the PCR instrument, place them on ice, and prepare the reaction system according to Table 9 below: Table 9: Reagent Single reaction volume End Repair Reaction Product 50 μL NadPrep® Universal Stubby Adapter 2 μL Ligation Buffer 26 μL DNA Ligase 2 μL Total 80 μL

[0034] (3) Gently vortex to mix, centrifuge and then quickly place in a PCR instrument. Incubate at 20°C for 15 minutes (with the hot cap closed). After incubation, remove the instrument and immediately begin magnetic bead purification.

[0035] 3. First step: magnetic bead purification (1) Before use, the library purification magnetic beads NadPrep® SP Beads should be taken out from 4°C, thoroughly vortexed and mixed, and equilibrated at room temperature for at least 30 min. (2) Shake the purified magnetic bead suspension (Naonda) at room temperature for 1 min, and aliquot it into PCR reaction tubes at a rate of 40 μL (0.5 times the sample volume) / sample. (3) Transfer all the ligation product to the magnetic beads in the corresponding PCR reaction tube, gently shake to mix, and avoid generating air bubbles; (4) After letting the PCR reaction tube stand at room temperature for 5 min, place it on a magnetic rack for 5 min until the supernatant becomes clear. Carefully remove the supernatant; (5) Keep the PCR reaction tubes on the magnetic rack, add 150 μL of freshly prepared 80% ethanol to each tube, let stand at room temperature for 1 min, and discard the supernatant.

[0036] (6) Repeat step (5), and after instantaneous centrifugation, put the PCR reaction tube back into the magnetic rack and use a 10 μL pipette to remove as much residual ethanol solution as possible from the bottom of the tube. (7) Leave the PCR reaction tubes on the magnetic rack at room temperature for 5 minutes to allow all the ethanol to evaporate. Do not over-dry. (8) Add 20 μL of enzyme-free water to each tube, mix up and down 10 times with a pipette, and then proceed to the next step of PCR amplification with magnetic beads.

[0037] 4. PCR amplification using UDI adapter primers (1) Take out 2X HiFi PCR Master Pro Mix and NadPrep® Universal UDI-Index Primer Mix and place them on ice to thaw naturally, mix well, and centrifuge briefly for later use; (2) Prepare the reaction system according to Table 10 below in a 0.2 mL PCR tube placed on ice (add the following ingredients in order from top to bottom): Table 10: Reagent Single reaction volume Purified Bead Contained Ligation Product 20 μL 2X HiFi PCR Master Pro Mix 25 μL NadPrep® Universal UDI-Index Primer Mix 5 μL Total 50 μL

[0038] Place the PCR tubes into the PCR instrument according to Table 11 below and start the following program: Table 11:

[0039] 5. Second step: Magnetic bead purification (1) Before use, the library purification magnetic beads NadPrep® SP Beads should be taken out from 4°C, thoroughly vortexed and mixed, and equilibrated at room temperature for at least 30 min. (2) Shake the purified magnetic bead suspension at room temperature for 1 min, and aliquot it into new PCR reaction tubes at a rate of 50 μL (equal volume of sample). (3) Take out the PCR reaction product, centrifuge for 30s, transfer all the product to the magnetic bead in the corresponding PCR reaction tube, and gently shake to avoid generating bubbles. (4) After standing at room temperature for 5 minutes, place on a magnetic separator for 5 minutes until the supernatant is clear. Carefully remove the supernatant; (5) Place it on a magnetic separator, add 150 μL of freshly prepared 80% ethanol, let it stand at room temperature for 1 min, and discard the supernatant; (6) Repeat step (5) and remove as much residual ethanol solution as possible; (7) Leave the PCR reaction tubes on the magnetic rack at room temperature for 5 minutes to dry, ensuring that all ethanol evaporates completely; (8) Add 22 μL of enzyme-free water and gently shake. Incubate at room temperature for 5 min; (9) Place on a magnetic rack for 5 min, and take 20 μL of supernatant (library) for the next step of library enrichment. Take 1 μL and dilute it 10 times. Use Qubit and fragment analyzer to determine the library concentration and fragment size, respectively. The average fragment length of the library should be between 200bp and 400bp.

[0040] [Hybrid Capture] 1. Library hybridization (1) Library pool mixing scheme: A library pool often contains multiple different libraries. The amount of library to be added to the pool is calculated based on the library concentration, and the libraries are mixed to obtain the final library pool. The total amount of library in the pool is 1.5~6 μg, and it is recommended to establish a library pool with the same mass of samples. Refer to Table 12 below for library usage: Table 12: Number of pooled samples Amount per library Total amount of library per reaction 1 1000 ng 1000 ng 2 1000 ng 2000 ng 3 1000 ng 3000 ng 4 800 ng 3200 ng 8 500 ng 4000 ng 12 500 ng 6000 ng

[0041] (2) Calculate the amount of different libraries to be used, mix each library in a 1.5 mL centrifuge tube, shake gently, and centrifuge briefly for later use; (3) Add the prehybridization reagents shown in Table 13 below to the mixed sample, mix well, and centrifuge briefly, trying not to generate bubbles; Table 13: Reagent Volume per single reaction Human Cot DNA 5 μL NadPrep® NanoBlockers (for Illumina®) 2 μL

[0042] (4) Open the caps of the prehybridization reagent tubes and place them in a vacuum concentrator. After balancing, dry at 60°C until the solution inside the tubes is completely dry. The drying process takes about 30 minutes. (5) When the drying process is almost over, take out the probe RA Panel and let it thaw naturally on ice. After the probe is used for the first time, dispense it in small quantities as needed. (6) Prepare the hybridization reaction solution Mix according to Table 14 below. After shaking evenly, take 13 μL (ES Hyb #1 8.5 μL + Hyb #2 2.7 μL + ddH2O 1.8 μL) and add it to the bottom of the vacuum-concentrated and dried centrifuge tube. Then add 4 μL of RA Panel separately and shake at low speed to mix evenly to avoid generating bubbles. Table 14: Reagent Single reaction volume / ul ES Hyb #1 8.5 μL Hyb #2 2.7 μL ddH2O 1.8 μL RA Panel (probe sequence is SEQ ID NO. 1~246) 4 μL Total 17 μL

[0043] Briefly centrifuge and incubate at room temperature for 8 min. Mix again by low-speed vortexing, briefly centrifuge, and then transfer all the hybridization reaction mixture from each centrifuge tube to the wells of a newly labeled eight-tube set. Add 10 μL of sealing oil to each well, cap the tube, briefly centrifuge to remove air bubbles, and place it in a PCR instrument. Start the hybridization program shown in Table 15 below. (During PCR operation, the Streptavidin Beads used for the next capture step can be removed from the refrigerator, thoroughly vortexed, and allowed to equilibrate at room temperature for at least 30 min). Table 15: Temperature Time Hot lid 100℃ On 95℃ ∞ 95℃ 30s 65℃ 30 min-16 h 65℃ ∞

[0044] 2. Library capture and cleaning (1) According to the reaction number, dispense the cleaning reagent (amount of reagent for a single enrichment reaction) into a new eight-tube according to Table 16 below; Table 16:

[0045] (2) Place the eight tubes containing the pre-packaged cleaning reagents that require heating on the PCR instrument and preheat them to 65°C. (3) Prepare magnetic bead suspensions according to the number of hybridization reactions as shown in Table 17 below; Table 17: Reagent Single reaction volume / ul ES Hyb #1 8.5 μL Hyb #2 2.7 μL ddH2O 5.8 μL Total 17 μL

[0046] (4) Based on the number of hybridization reactions, prepare new 0.2 mL thin-walled eight-tube sets with the required number of alleles. Shake the StreptavidinBeads for 2 min to ensure complete mixing. Dispense 50 μL of magnetic beads into each well of the eight-tube set; (5) Add 100 μL of room temperature ES Wash Buffer to each well of the eight-tube strip, gently pipette to mix 10 times, centrifuge briefly, place on a magnetic rack for 1 min, and wait for the liquid to become completely clear. Use a pipette to discard the supernatant. Remove the eight-tube strip from the magnetic rack. (6) Repeat step (5) once; (7) Add 17 μL of magnetic bead suspension to each well of the eight-tube, gently blow and aspirate to mix, and transfer all the magnetic bead suspension to a new 0.2 mL thin-walled eight-tube; (8) Place the 0.2 mL thin-walled eight-tube strip containing the suspended magnetic beads into a PCR instrument and incubate at 65°C for 5 min; (9) After the hybridization process of the above library (7), open the PCR instrument lid. On the PCR instrument, open the lids of the magnetic beads and the eight-tube hybridization product respectively. Then take 100 μL of pipette and adjust it to 30 μL. Quickly transfer all the heated magnetic bead mixture to the wells where the corresponding hybridization products are located. Immediately and slowly pipette to mix 10 times. Try to avoid generating air bubbles during the pipetting and mixing process. Note: Quickly transferring the magnetic bead suspension in the PCR instrument to the hybridization product is a key step. You must directly take samples on the PCR instrument. Do not remove the hybridization tube to prevent the temperature of the hybridization solution from dropping. (10) Then replace the cap on the hybridization product tube, cover the PCR instrument, and incubate at 65°C for 45 min. Every 10 min, open the PCR instrument and then open the cap on the eight-tube hybridization product tube. Gently blow and aspirate 10 times to ensure that the magnetic beads are completely resuspended. Try to avoid generating air bubbles during the blowing and aspirating process. (11) After incubation, open the PCR tube cap on the PCR instrument and add 150 μL of 65℃ ES Wash Buffer. Gently blow and aspirate 20 times to mix the hybridization system containing magnetic beads. (12) Place the PCR tube on a magnetic rack for 30 seconds. After the liquid is completely clear, use a pipette to remove the supernatant, centrifuge briefly, and then use a 10 μL pipette tip to remove a small amount of residual buffer. (13) Remove the PCR tube from the magnetic rack and place it into the PCR instrument. Add 150 μL of 65℃ ES Wash Buffer again, gently blow and aspirate 20 times to mix evenly, and incubate at 65℃ for 5 min. (14) Place the PCR tube on a magnetic rack for 30 seconds. After the liquid has completely clarified, aspirate and discard the supernatant. Centrifuge briefly, then use a 10 μL pipette tip to remove a small amount of residual buffer. Add 150 μL of room temperature ES Wash Buffer and gently pipette 20 times to mix (avoid vortexing). Transfer all the reaction solution with the magnetic beads to a new 0.2 mL eight-tube and incubate at room temperature for 2 min. During this time, vortex for 30 seconds and let stand for 30 seconds, alternating between the two to ensure thorough mixing. (Note: After discarding the 65℃ ES Wash Buffer, you must use a small pipette tip to remove any remaining buffer. After adding room temperature ES Wash Buffer and mixing by pipetting, you must transfer the reaction solution to a new eight-tube; otherwise, it may affect the quality of subsequent sequencing data.) (15) After briefly centrifuging the PCR tube, place it on a magnetic rack for 30 seconds. Once the liquid is completely clear, aspirate and discard the supernatant. Centrifuge briefly again, then use a 10 μL pipette tip to remove a small amount of residual buffer. Add 150 μL of room temperature ES Wash Buffer and gently pipette 20 times to mix. Incubate at room temperature for 2 minutes, vortexing for 30 seconds and then letting it stand for 30 seconds, alternating between the two to ensure thorough mixing. (Note: Control the vortex mixer speed at around 1500 rpm to ensure thorough mixing; otherwise, it may affect the quality of sequencing data.) (16) After the PCR tube is briefly centrifuged, place it on a magnetic rack for 30 seconds. After the liquid is completely clear, remove the supernatant, centrifuge briefly, and then use a 10 μL pipette tip to remove a small amount of residual buffer. (17) Remove the PCR tube from the magnetic rack, add 22.5 μL of ddH2O, and proceed to the next step of PCR amplification with the magnetic beads (Note: including the magnetic beads, do not discard the magnetic beads).

[0047] 3. PCR amplification (1) Prepare and label new eight-tube sets according to the number of reactions. Add (25 μL VAHTSUltra Amplification Mix (Novozymes) + 2.5 μL NadPrep® Amplification Primer Mix (Naonda) = 27.5 μL) to each well of the eight-tube set. Then remove NadPrep® SP Beads (Naonda) from the 4°C refrigerator, shake well and mix thoroughly until equilibrate to room temperature for at least 30 min. (2) Take 22.5 μL of magnetic bead suspension obtained in step (17) of the above library capture and cleaning process and add it to each well of the corresponding marked eight-tube. After covering the tube, shake and mix at low speed and then centrifuge briefly. (3) Place the eight-tube strip on the PCR instrument, heat the lid to 105℃, and amplify according to the reaction program in Table 18 below: Table 18:

[0048] 4. Magnetic bead purification (1) Vortex thoroughly to mix the pre-equilibrated DNA purification magnetic beads NadPrep® SP Beads. Take a clean PCR reaction tube, add 75 μL (1.5*) DNA purification magnetic beads, and after the PCR is completed, centrifuge the PCR tube briefly. Then transfer all the solutions in the PCR tube to the PCR reaction tube and vortex thoroughly to mix. (2) Incubate the PCR reaction tubes at room temperature for 5 min; (3) Place the PCR reaction tube on a magnetic rack for 1 min, and remove the supernatant after the solution becomes clear; (4) Keep the PCR reaction tube on the magnetic rack, add 150 μL of freshly prepared 80% ethanol, incubate for 1 min, and discard the supernatant; repeat the washing with 80% ethanol once (total 2 times). (5) After the PCR reaction tube is centrifuged briefly, place it on a magnetic rack and carefully remove the residual ethanol with a 10 μL pipette tip. Let it stand at room temperature for 5-10 min or until the magnetic beads are dry. Avoid cracking. (6) Remove the tube from the magnetic rack and add 22 μL of nuclease-free water, gently shake to mix, and incubate at room temperature for 5 min; (7) Place the PCR reaction tube on a magnetic rack for 3 min or until the solution becomes clear; (8) Transfer 20 μL of supernatant to a clean 1.5 mL centrifuge tube, and label the tube cap with the library name and the date of library construction. Note: If the purified enriched library cannot be sequenced immediately, it can be stored at -20℃ or below for 30 days; (9) Take another 1 μL and use the Qubit® dsDNA HS Assay Kit (Vazyme) to determine the library concentration. The library concentration should be greater than 1 ng / μL. At the same time, the library fragment length distribution can be detected using the 2100 fragment analyzer (Agilent). The average fragment length should be 200-400 bp.

[0049] The panel hybridization capture probe sequences for hematologic malignancy fusion genes are shown in Table 19 below: Table 19 Gene Probe Sequence ID Sequence BCL10 pro-c85731458 NO:1 TTTATGCAAAGCTTAATTTTATTGTCTATGTAGTTAGAAAACAGTCTGAAAGAAAACTCAGTGTTAAAACTTATCTATATATAGGTGTTATTGTATATCTAAATTATAATCTTTGATTCAATTTTTAAAAAGGTGTCCACAAATTTAATT BCL10 pro-c85731608 NO:2 TTAAAGTCTGCTTCTGCTTTTTAAAGCACCTTCCTTAAATGTAACCTATTTTCTTTCTAACACCACTATTTTTGCTATGCATTGGAGGTGAAACATTTCTCTATAATCCTTAGAAAGTGCTTATACTAAAAAAAATCCAATCTTTAAAAA BCL10 pro-c85731758 NO:3 TTTTTAAAGCTAATATGCCAATCTTCTTCATGTCGGATAAATTCATCAGTCCATCCAGAAATGCAACTCAATCTCACAACCTGAAGTAATGTTTTCTATAGATAATGGCACGTATTAAAAAAAGTTTTAGATACTTCCTACTGAAAATTT BCL10 pro-c85731908 NO:4 GGAACTCCAAAATAGCACTCACTACTTTAAAAAAATTTTATTGTTGATAGCAAAATTTCCATTTCTATGTTTTTGCAATTAATAATAGAAATATATAGGAGACAATGTCAGGAAACAGATAAAATTGTAATTTAAATAAAAACAAACAGT BCL10 pro-c85732058 NO:5 GAGAGCATAAGATTTATAAACATGTAAAATTAGTCAAATTTTGTTTTACAACAAAAAGACAAATCTGGCTAATGGCCCACTTGTTCAAATAGCATTAAAAACAGAATTGTCACACACACCAAAAATAATTTGTTCCAGTTATTTCAACTG RBM15 pro-c110881998 NO:6 GAGACTGTAGAAGAGAGAGCAATTGGCCAATGAGGACTGCGGGGCGGGACCCTGTGCCGCGGCGGAGTCCAAGATGGCGGCGTGCGGTTCCGCTGTGTGAAACGAGCGCGGGGCGGCGGGTTACTCAGCTCCGCGGAGACGACCTCCGAC RBM15 pro-c110882148 NO:7 GACCCGCAACAATGAAGGGAAAAGAGCGCTCGCCAGTGAAGGCCAAACGCTCCCGTGGTGGTGAGGACTCGACTTCCCGCGGTGAGCGGAGCAAGAAGTTAGGGGGCTCTGGTGGCAGCAATGGGAGCAGCAGCGGAAAGACCGATAGCG RBM15 pro-c110882298 NO:8 GCGGTGGGTCGCGGCGGAGTCTCCACCTGGACAAGTCCAGCAGTCGAGGTGGCAGCCGCGAGTATGATACCGGTGGGGGCAGCTCCAGTAGCCGCTTGCATAGTTATAGCTCCCCGAGCACCAAAAATTCTTCGGGCGGGGGCGAATCGC RBM15 pro-c110882448 NO:9 GCAGCAGCTCCCGGGGTGGAGGCGGGGAGTCACGTTCCTCTGGGGCCGCCTCCTCAGCTCCCGGCGGCGGGGACGGCGCGGAATACAAGACTCTGAAGATAAGCGAGTTGGGGTCCCAGCTTAGTGACGAAGCGGTGGAGGACGGCCTGT RBM15 pro-c110882598 NO:10 TTCATGAGTTCAAACGCTTCGGTGATGTAAGTGTGAAAATCAGTCATCTGTCGGGTTCTGGCAGCGGGGATGAGCGGGTAGCCTTTGTGAACTTCCGGCGGCCAGAGGACGCGCGGGCGGCCAAGCATGCCAGAGGCCGCCTGGTGCTCT FOXP1 pro-c71003858 NO:11 TTTTACTACCAGCGTGAACAACCAGCATTTTTATTGCATTTGAGAATGCTTATAATGTCAGTAATTAGTACTGACTACACAACATTTTTTTTATTGTCTGTATCCGCAGACATGGAATGATGGAATTACAGTTGATGTCAAGGAATGAGT FOXP1 pro-c71004008 NO: 12 TTCTTTTATGCCTTATCAAAACAAAACAAAACAAAACAAAAAAATTCTTGTTACTGGCAGCACATATACATGAAGCACCATGCTCACAGTCCGGACTGTATCATCTTCATCAAGGCTTATGGGTAGCAGAGATTGCGTGAGCTACACTGG FOXP1 pro-c71004158 NO: 13 GCCCAAGAAATGCCTTCAGCATTGTAAATCTGATTTTCAGGATAAAGAAGCAAAACTGACTTTGAAGACATCCAGAATTTCAGGAGGCTATGTCATTAACCAAAAGGTACCAAAAAGATTCAAAATCTGAATAAGCACACTCTTCTTTGT FOXP1 pro-c71004308 NO: 14 GCCTTTGGAAACGGAGTTCAGCTGGAATATGGAAAAGAGAGGAAATATTTTTTAACTCTATTTTTTTTCATGAGGAAAAAAAAGCTAGTGATTTACAGCCTATCTTGGGTTAAGAATCCAAGGTTTTCTTTACTAGGTCTGACTGGATGC FOXP1 pro-c71004458 NO: 15 TGGGGATGGTGTTAGAGCTGTCCAAAGGTGGCAGGACTGGTGGTAACTCTTCACGTATGTACATAAGCCTTGATATTCCATTTTGTGGCTGGTCCAAGGGGCAGCCTAACTCATCTTACAAGACGGACTCTAGGGACAGGTAGGTTGGAT GATA2 pro-c128198264 NO: 16 GGGCATTCACAAAAAGTATTTTATTATTCTTAACAGTACTCACTTTAAAGGAATAAGAGGATAGCATACATTTTTTACAGACAATATATAAATGTTGTACATAATTAACAATAACTTAGTTCACTAATCCAAAATAAAACAAGCCAAATA GATA2 pro-c128198414 NO:17 AAACATAAAAACAGAAAATACTGCCGATTCTTTTTCTTATGCGGACACTAGTACAAAATAAGTTACTTCTGGCCGTGGTGCTCCCTGCAGCGACTGCCCGCCCATATTGCACTTGGTCACTACATCAGCACAATCCTCCTCCTGGGCCAG GATA2 pro-c128198564 NO:18 GGGCCCCTCACAGGCCACCACACCCCGCCGTCACCGCATACAGAATCTAAGCTCGGGACACGTTTATATACAGCTGTACCTTGGGAAGGATCCAGACAGCCACAGTAAAGCTGGACCTGAGACCCCAGCAGGGACACAGCCTCTCCCTGG GATA2 pro-c128198714 NO:19 GTCCTGGTCCTGACCTGCCCCCAACTCCTGCCTTCACCCCCTGCCAGGCCAGCAAATGCCAACCAGCCCCCAGAAACCAAGGGCAGCAAAGGCCCCTCCCCACCCCCAGCTTTCATACTAGGGCTGTGGGATCCCAGCTCTTTTCCAAAA GATA2 pro-c128198864 NO:20 AGAATTGCAAAGCTCCAACCTTGTGTGTAGGTTTTATTCCTTTCATAGCAGGGCTCCTGTGGCTACGTACAATCAACTGGACCCAAAAAGAGTTTAAAAATAAAACAATTAACTCATCAGCGAGTTAAGCCTATGCATTTTTTTAATATT MLF1 pro-c158288982 NO:21 GAGTGAGGCGTCGTCCGTACTGGAGGCTAGCTCTTGTCGCGGCCGGCGCGAGTTAACATCGTTTTTCCAATCTGTCCGCGGCTGCCGCCACCCAAGACAGAGCCAATGTTCAGGATGCTGAACAGCAGTTTTGAGGATGACCCCTTCT MLF1 pro-c158300444 NO:22 AGACCATGCAAGCAAGAAGAGAATGGCATGAAATATAAAGTGTTGAGAGGAAAAAAACCCACTGACCTAGAATCTGTATCCTGCAAAATTATCCTTTAAAAGTCAAGAAAATAGACTGTCTCAGACAAACAAATTTGGTGAAT MLF1 pro-c158317802 NO:23 ATAAAGGAAACCAGGAAAGCAATGAGAGATTCTGACAGTGGACTAGAAAATGGCTATTGGTCATCATCCATGACCGAGCTCATGTCATTAAAGTCAAAGAACAAGAACTGGAGATGAAGGTCAACCAGGAGTTCATCAAT MLF1 pro-c158322885 NO:24 GGAGAAACCTCAACAAAGTCCAGCCATTGAACATGGAAGGAGATCAAATGTTTTGGGGACAAACTCCACATCAAAGGCTCATCTGTGAAAAGCAACAAAAATAAATAGCCATGCATTTGATTTGTTTAGTTTTGATTGTTTTAACAGT MLF1 pro-c158323035 NO:25 TAGTAATGGTGCTGGGTAATAAGCATAAGACCAATCTCTTGCTGTTAAATCAGTTCTGTCCTTGGCAACTTTCTTCTGATATCTGAATGTTCATGAAGGTCCTAGCTTTATATTGTCCCTCTTTTAGGAATAAAATTTGATTTTCAACA MECOM pro-c168801294 NO:26 TCCTTTTTGTAAGTCTTTATTTTTGTTGCTCCTCCCATAGTAATGCACTGAAGGCATAACAGTTTATTGTACAAAGCATTTGAGAAAGTACCTCAACTTGCTGATTTTCAAAATGATTATACAAAAAAAAAATC MECOM pro-c168801444 NO:27 TGGTTCCTCAATAAAGGGCAAAATACTGATACAGTCTGTTATTTACTTCTCTCTTTTTTAACATAAGGTTGAACATAATCGCAAGCTTGCGACAACCAGCAATATGGA MECOM pro-c168801594 NO:28 GTACAGTTTTTAATCAGAATCATGCTTCCGAAAGAAATTATTATCGTTTATACAATTGAATCGATTTCAGTTACAAAACTAAGTTGCATCTATTCGTATTTAGTTAGTTAAAGGAAAAAAAAAAAAAAAAAAAAGGA MECOM pro-c168801744 NO:29 ATGTTAAGAAAAGCCAAACACCATTTGGCACTCTCATACACAGGTCGGGTCACCTTGGTCCTTGAAATTCGGAAAGGGGTGTGCCTCACGTCATAAAGTAAGTAGTGGCTGACTGGACAGTGCTATTTATCAACAAACAATAGTTTGCC MECOM pro-c168801894 NO:30 AACAAAAATACATGATACACGCAACACAAAAAAAAAAACATTAAAAACAGTGATTGTCTAAAATTAAGATGTTATTACAAGGATTTGGCAAATTTAGTGGTACAGCGGTACTGGTGATGAATAGGTTACTTCA BCL6 pro-c187439164 NO:31 CCAGTTTGACTTTTCAACATTTTATTCTTATATTTGTACAGCTATATTTTACAACGCGGTAATGCAGTTTAGACACAGCCAAACCCTGTCTCCGGAGTAGTTATAACACAAGCATGACGCAGAATGGGATGAGACAAACATTCCCAAAGA BCL6 pro-c187439314 NO:32 GAGTTTAGTTAGTAACAACTTCAAGTCCCTGTGTCTGCCTACACTTCAAAAAGGGATGGTGCACGCTCGCCCATCATTGAAAACTTCCGTGAAAAAAGGCACCGTGAGGACACGTTGTACGGGTATATACAAACTGAAAACTAGAACAAA BCL6 pro-c187439464 NO:33 ATTACACATTTTTCCTTCTGCAGATTTTTTTGTTCTTTTTGTTTTTTTTAATACACACTTTGTAAATTGTAAACCTTCACTTGCAAAAAAATACAAATACACTGAGGCATTTTAGACAAAATATTTTCTTACTTATACAGATGCAATACT BCL6 pro-c187439614 NO:34 TAAAATATTCTCTTAAGTGCTCTTTCTCAATAAAGATTCTCAGATCCGTGTCTGCCTGCAGATACAAAATCGAGCCTTTAACGCAGTTTTATATTTTTAATATATCTTTTTTAGGTTTATATATATTTATTTTATATATATATATATTTA BCL6 pro-c187439764 NO:35 TATATTTACAACTCTGCCATATATTCCTTCACCTTTGGTTAAAAAAATTAAAGCCCTCTCTTTGACAACATACTGAAGTCTTGTCTTTTAAAAGAATGCACATTTACATACAAAAGAAACATCTGTTCCCACAAAACATATACATTCCTC FGFR3 pro-c1795019 NO:36 AGTGCGCGGTGGCGGCGGCGTCGCGGGCAGCTGGCGCCGCGCGGTCCTGCTCTGCCGGTCGCACGGACGCACCGGCGGGCCGCCGGCCGGAGGGACGGGGCGGGAGCTGGGCCCGCGGACAGCGAGCCGGAGCGGGAGCCGCGCGTAGCG FGFR3 pro-c1795559 NO:37 TCTCCCGAGCGGCGCCCGCCTCCCGCCGGTGCCCGCGCCGGGCCGTGGGGGGCAGCATGCCCGCGCGCGCTGCCTGAGGACGCCGCGGCCCCCGCCCCCGCCATGGGCGCCCCTGCCTGCGCCCTCGCGCTCTGCGTGGCCGTGGCCATC FGFR3 pro-c1800980 NO:38 AAGTCCCGGGCCCAGAGCCCGGCCAGCAGGAGCAGTTGGTCTTCGGCAGCGGGGATGCTGTGGAGCTGAGCTGTCCCCCGCCCGGGGGTGGTCCCATGGGGCCCACTGTCTGGGTCAAGGATGGCACAGGGCTGGTGCCCTCGGAGCGTG FGFR3 pro-c1803093 NO:39 GGGCCCCTTACTGGACACGGCCCGAGCGGATGGACAAGAAGCTGCTGGCCGTGCCGGCCGCCAACACCGTCCGCTTCCGCTGCCCAGCCGCTGGCAACCCCACTCCCTCCATCTCCTGGCTGAAGAACGGCAGGGAGTTCCGCGGCGAGC FGFR3 pro-c1803561 NO:40 AGCGCTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTGCCGGCCAACCAGACGGCGGTGCTGGGCAGCGACGTGGAGTTCCACTGCAAGGTGTACAGTGACGCACAGCCCCACATCCAGTGGCTCAAGCACGTGGAGGTGAATGGCAGCA NSD2 pro-c1873119 NO:41 GGCGCCGCGCGCGAGAGCCTCGGCCTGGCCGCGCTGCGCCCGCCGCCGCCGCCGCCCCCTCCCCGCCTGGGCCCTACCGCCGCACGGCCCCGGCCCCCTCCCAGCCTGCCGCTCCGGAGAGCCGCCCGCCGAGGATGCGACGCACCGCAG NSD2 pro-c1902352 NO:42 TGTTCTAAGAACGGAAGCATCTGGGCTGGATGGAATTTAGCATCAAGCAGAGTCCCCTTTCTGTTCAGAGTGTTGTAAAGTGCATAAAGATGAAGCAGGCACCAGAAATCCTCGGCAGTGCCAACGGGAAGACTCCGAGCTGCGAGGTGA NSD2 pro-c1902502 NO:43 ACCGCGAGTGTTCTGTGTTCCTCAGCAAAGCCCAGCTCTCCAGTAGCCTGCAGGAGGGGGTCATGCAGAAGTTTAACGGCCACGACGCCCTGCCCTTTATTCCAGCCGACAAGCTGAAAGATCTTACTTCCCGGGTGTTTAATGGAGAAC NSD2 pro-c1902652 NO:44 CCGGCGCACACGATGCCAAACTGCGTTTTGAGTCCCAGGAAATGAAAGGGATTGGGACACCCCCTAACACTACCCCTATCAAAAATGGCTCTCCAGAAATTAAGCTGAAAATCACCAAAACATACATGAATGGGAAGCCTCTCTTTGAAT NSD2 pro-c1902802 NO:45 CTTCCATTTGTGGTGACAGTGCTGCTGATGTGTCTCAGTCAGAAGAAAATGGACAAAAACCAGAAAACAAGGCGAGAAGGAACAGGAAGAGGAGCATAAAATATGACTCCTTGCTGGAGCAGGGCCTTGTCGAAGCAGCTCTTGTGTCTA FIP1L1 pro-c54243807 NO:46 GCGCATGCGCAGACGGACCTGCGCTGGAGGCTTCATCTTTGCCGCCGCTGCCGTCGCCTTCCTGGGATTGGAGTCTCGAGCTTTCTTCGTTCGTTCGTCGGCGGGTTCGCGCCCTTCTCGCGCCTCGGGGCTGCGAGGCTGGGGAAGGGG FIP1L1 pro-c54294193 NO:47 GTCCTTTCTGAAAGATCTGCTACTGAAGTAGACAACAATTTTAGCAAACCACCTCCGTTTTTCCCTCCAGGAGCTCCTCCCACTCACCTTCCACCTCCTCCATTTCTTCCACCTCCTCCGACTGTCAGCACTGCTCCACCTCTGATTCCA FIP1L1 pro-c54319086 NO:48 TTGCCTTTCCCCATCTTCCTGGTTCTGCTCCTTCGTGGCCTAGTCTTGTGGACACCAGCAAGCAGTGGGACTATTATGCCAGAAGAGAGAAAGACCGAGATAGAGAGAGAGACAGAGACAGAGAGCGAGACCGTGATCGGGACAGAGAAA FIP1L1 pro-c54325468 NO:49 TAATAGTAGACGTCGCCATGAAAGTGAAGAAGGAGATAGTCACAGGAGACACAAACACAAAAAATCTAAAAGAAGCAAAGAAGGAAAAGAAGCGGGCAGTGAGCCTGCCCCTGAACAGGAGAGCACCGAAGCTACACCTGCAGAATAGGC FIP1L1 pro-c54325618 NO:50 ATGGTTTTGGCCTTTTGTGTATATTAGTACCAGAAGTAGATACTATAAATCTTGTTATTTTTCTGGATAATGTTTAAGAAATTTACCTTAAATCTTGTTCTGTTTGTTAGTATGAAAAGTTAACTTTTTTTCCAAAATAAAAGAGTGAAT PDGFRA pro-c55127261 NO:51 GGCTGAGCCTAATCCTCTGCCAGCTTTCATTACCCTCTATCCTTCCAAATGAAAATGAAAAGGTTGTGCAGCTGAATTCATCCTTTTCTCTGAGATGCTTTGGGGAGAGTGAAGTGAGCTGGCAGTACCCCATGTCTGAAGAAGAGAGCT PDGFRA pro-c55127411 NO:52 CCGATGTGGAAATCAGAAATGAAGAAAACAACAGCGGCCTTTTTGTGACGGTCTTGGAAGTGAGCAGTGCCTCGGCGGCCCACACAGGGTTGTACACTTGCTATTACAACCACACTCAGACAGAAGAGAATGAGCTTGAAGGCAGGCACA PDGFRA pro-c55129833 NO:53 ACCCAGATGTAGCCTTTGTACCTCTAGGAATGACGGATTATTTAGTCATCGTGGAGGATGATGATTCTGCCATTATACCTTGTCGCACAACTGATCCCGAGACTCCTGTAACCTTACACAACAGTGAGGGGGTGGTACCTGCCTCCTACG PDGFRA pro-c55133455 NO:54 AAAGGCAAAGGCATCACAATGCTGGAAGAAATCAAAGTCCCATCCATCAAATTGGTGTACACTTTGACGGTCCCCGAGGCCACGGTGAAAGACAGTGGAGATTACGAATGTGCTGCCCGCCAGGCTACCAGGGAGGTCAAAGAAATGAAG PDGFRA pro-c55133718 NO:55 AGAAAGGTTTCATTGAAATCAAACCCACCTTCAGCCAGTTGGAAGCTGTCAACCTGCATGAAGTCAAACATTTTGTTGTAGAGGTGCGGGCCTACCCACCTCCCAGGATATCCTGGCTGAAAAACAATCTGACTCTGATTGAAAATCTCA AFF1 pro-c87856162 NO:56 AGCCAGCTAGCGAGCGACGGGCGCGCGCGGCCCCTGCGCACTCGGCCGCCGCCTGCGCGCGTTGCGGCCGCAGCTGCACCTTTGCCTCCGCGGTTCCGCAGCCGAGCCCCGGGAGGAGGCGCTCCCCTGCGTCGTCCGCACTTGCGCCCT AFF1 pro-c87967846 NO:57 ACAGCAAAAGGTGATGAGCTGTCTAGTCGAATACAGAACATGTTGGGAAACTACGAAGAAGTGAAGGAGTTCCTTAGTACTAAGTCTCACACTCATCGCCTGGATGCTTCTGAAAATAGGTTGGGAAAGCCGAAATATCCTTTAATTCCT AFF1 pro-c87967996 NO:58 GACAAAGGGAGCAGCATTCCATCCAGCTCCTTCCACACTAGTGTCCACCACCAGTCCATTCACACTCCTGCGTCTGGACCACTTTCTGTTGGCAACATTAGCCACAATCCAAAGATGGCGCAGCCAAGAACTGAACCAATGCCAAGTCTC AFF1 pro-c87968146 NO:59 CATGCCAAAAGCTGCGGCCCACCGGACAGCCAGCACCTGACCCAGGATCGCCTTGGTCAGGAGGGGTTCGGCTCTAGTCATCACAAGAAAGGTGACCGAAGAGCTGACGGAGACCACTGTGCTTCGGTGACAGATTCGGCTCCAGAGAGG AFF1 pro-c87968296 NO:60 GAGCTTTCTCCCTTAATCTCTTTGCCTTCCCCAGTTCCCCCTTTGTCACCTATACATTCCAACCAGCAAACTCTTCCCCGGACGCAAGGAAGCAGCAAGGTTCATGGCAGCAGCAATAACAGTAAAGGCTATTGCCCAGCCAAATCTCCC PDGFRB pro-c149493401 NO:61 TGTGAGTGAGAAGCACCAGGTTTAATATTAAAATCTTTCCCTTAAAAAAAAGTACACAGATAGAAAAACTCAACAGCATACAAAATAGCATTTCTGCTGTATAAATGTGAGTTAACGTGAGTCCTAAAAATATTTGTAAACCTAGGTGAT PDGFRB pro-c149493551 NO:62 TATATCTTTGGTACCGTATTGAGAACCCACTCTCCCTCCTTGGACCAACTCTGGGGATCCCAGGGAAGTAAGGTGCCAACCTGCAATGCAGGGTTGGTGCCCAGAGGGGTTGAGCTTCCTGCACCCCCTTCCCAACCCTCCCCGTGCGGG PDGFRB pro-c149493701 NO:63 GGCGTCTTGGCTACAACCCTGACTCCCCTGGCACCTCCAATGCCCACTGGGCTGGGGACAATGAGATGTCACTGCTAGGTCTGGGCAGTGACAAAACCATACCCCCATGGAGCGCTGCCTCAGGGATGGGGCAGCTGGTGGGTGAGGGGC PDGFRB pro-c149493851 NO:64 AAACCTCCAAAGCGCCCACCTCTCACATCCTTCTTGGGGTACCCATCTGAAGCTTCTTGTCTTTTACAAGACATAACTGTCCTCACTGTCCATTCTGTCTTCGACGCAGACCTCGGCACAGGTGCTGGAGTGGTGGGCTGCAGGAGGTGA PDGFRB pro-c149494001 NO:65 TCTCATTTGCCCTCTTTGTCCCTTTTGCCCAGGATACCCCCGGAGGTCATCGTGGGTAGTGAAAGAGGTCCCGTGGTCTTCCCATGTCCCTGGGGCAGCTGCTGGGGGCTTTCCAGGACACCGGCTGTCACTCTAAGTCAAGGCCTGTGC TLX3 pro-c170736251 NO:66 GCGCACTCTTGGCAAAGTTTCAGTGCGACGAGAGGCGCCGGGCGCTCCATGGCCGCGCCGTAACGGGGACCCAGCCGCCTCCCCGCCCAGCCCAGCCCAGCCCTTCCGCCCGCCCAGGATGGAGGCGCCCGCCAGCGCGCAGACCCCGCA TLX3 pro-c170736401 NO:67 CCCGCACGAGCCCATCAGCTTCGGCATCGACCAGATCCTTAACAGCCCGGACCAGGACAGCGCACCCGCCCCGCGGGGCCCCGACGGCGCCAGCTACCTGGGAGGGCCCCCCGGGGGCCGTCCGGGCGCCACATACCCGTCTCTGCCCGC TLX3 pro-c170736551 NO:68 CTCCTTTGCGGGCCTCGGCGCGCCCTTCGAGGACGCGGGATCTTACAGTGTGAACCTGAGCCTAGCGCCCGCAGGCGTGATCCGGGTGCCGGCGCACAGGCCGCTGCCCGGGGCCGTGCCACCGCCTCTGCCAAGCGCGCTACCCGCCAT TLX3 pro-c170737153 NO:69 CGGCGGCCGCACTCACGCCCTTCACCGTGACCCGGCGCATCGGCCACCCCTACCAGAACCGGACGCCGCCCAAGCGTAAGAAGCCGCGCACGTCCTTTTCCCGGGTGCAGATCTGCGAGCTGGAAAAGCGCTTCCATCGCCAGAAGTACC TLX3 pro-c170738392 NO:70 GCGGCAGACGGCGGAGGAGCGGGAGGCGGAGCGGCAGCAGGCGAGCCGGCTCATGCTGCAGCTGCAACACGACGCCTTCCAAAAGAGCCTCAACGACTCCATCCAGCCTGACCCGCTCTGTCTGCACAACTCGTCACTCTTTGCTCTGCA NPM1 pro-c170837530 NO:71 GCTATTCAAGATCTCTGGCAGTGGAGGAAGTCTCTTTAAGAAAATAGTTTAAACAATTTGTTAAAAAAATTTTCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTATAATGCAGAGTGAGAACTTTCCCTACCGTG NPM1 pro-c170837680 NO:72 TTTGATAAATGTTGTCCAGGTTCTATTGCCAAGAATGTGTTGTCCAAAATGCCTGTTTAGTTTTTAAAGATGGAACTCCACCCTTTGCTTGGTTTTAAGTATGTATGGGAATGTTATGATAGGACATAGTAGTAGCGGTGGTCAGACATGG NSD1 pro-c176631120 NO:73 TTTCCAACCGGAGGCCCTATCGGCAGTACTACGTGGAGGCTTTTGGAGATCCTTCTGAGAGAGAGCCTGGGTGGCTGGAAAAGCAATCGTCATGTTTGAAGGCAGACATCAATTCGAAGAGCTACCTGTCCTTAGGAGAAGAGGGAAACAGA NSD1 pro-c176636636 NO:74 GTTCCTCAGAAAATTTTGAGTAAATGGGAAGCCAGTGTTGGACTTGCAGAACAGTATGATGTTCCCAAGGGGTCAAAGAACCGAAAATGTATTCCTGGTTCAATCAAGTTGGACAGTGAAGAAGATATGCCATTTGAAGACTGCACAAAT NSD1 pro-c176636786 NO:75 GATCCTGAGTCAGAACATGACCTGTTGCTTAATGGCTGTTTGAAATCACTGGCTTTTGATTCTGAACATTCTGCAGATGAGAAGGAAAAGCCTTGCGCTAAATCTCGAGCCAGAAAGAGCTCTGATAATCCACAAAGGACTAGTGTGAAA NSD1 pro-c176636936 NO:76 AAGGGCCACATACAATTTGAAGCACATAAAGATGAACGGAGGGGAAAGATTCCAGAGAACCTTGGCCTAAACTTTATCTCTGGGGATATATCTGATACGCAGGCCTCTAATGAACTTTCCAGGATAGCAAATAGCCTCACAGGGTCCAAC NSD1 pro-c176637086 NO:77 ACTGCCCCAGGAAGTTTTCTGTTTTCTTCCTGTGGAAAAAACACTGCAAAGAAAGAATTTGAGACTTCAAATGGTGACTCTTTATTGGGCTTGCCTGAGGGTGCTTTGATCTCAAAGTGTTCTCGAGAGAAGAATAAACCCCAACGAAGC DUSP22 pro-c348102 NO:78 CCTGGCCGGGGTCTCCAGGAGCGTGACACTGGTGATCGCATACATCATGACCGTCACTGACTTTGGCTGGGAGGATGCCCTGCACACCGTGCGTGCTGGGAGATCCTGTGCCAACCCCAACGTGGGCTTCCAGAGACAGCTCCAGGAGTT DUSP22 pro-c348768 NO:79 TATCGGCAGTGGCTGAAGGAAGAATATGGAGAGAGCCCTTTGCAGGATGCAGAAGAAGCCAAAAACATTCTGGGTAAATATAAGGAGCAAGGGCGCACAGAGCCCCAGCCCGGCGCCAGGCGGTGGAGCAGTTTTCCGGCACTGGCTCCG DUSP22 pro-c348918 NO:80 CTGACCTACGATAATTATACGACGGAGACCTAACGCAAGCGACCTGCTGCCTTCCTTCCCACTGCTTGTCTTCAGTGTGCCCGGCTGGGCAGGGGTGCGGTGGTGGTGGCCGATGAGGACAGGAAAGGGAGATAGCCAGGGCGAGGTGGG DUSP22 pro-c349068 NO:81 GCGAGGGCTCCTTCCCCCAAGCAACACCGCCCAGCCCTGCTCCAGGCCCCTGCACTCCGCCCACCCCTACCCTGGCTGCACCTGAGCTTGCTGCCCCTGGGGATGTTGCCCAGTGGCTGTGCACTGCTCTGTGCACGTGCGTGTGTGTGA DUSP22 pro-c349218 NO:82 GTGCACTTGTGTGTGGGTGACTAAGTGGATGCATGTGTGTGCCTGTGTGAGTGAGGGTATGTGCACCTAAGTGTGTACATGTGTGTATGTTGTGAAAGTGTCTGTGCACATGAATGTTTGTGTGTGTGTGAACTCTTTCTTACTGCTGGA IRF4 pro-c393097 NO:83 TGCAGAGCAGAGCGGGCGGAGGACCCCGGGCGCGGGCGCGGACGGCACGCGGGGCATGAACCTGGAGGGCGGCGGCCGAGGCGGAGAGTTCGGCATGAGCGCGGTGAGCTGCGGCAACGGGAAGCTCCGCCAGTGGCTGATCGACCAGAT IRF4 pro-c394820 NO:84 GCTTGGGCACTGTTTAAAGGAAAGTTCCGAGAAGGCATCGACAAGCCGGACCCTCCCACCTGGAAGACGCGCCTGCGGTGCGCTTTGAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACATCTCAGACCCGTAC IRF4 pro-c401423 NO:85 ACTGCCGGCTGCACATCTGCCTGTACTACCGGGAAATCCTCGTGAAGGAGCTGACCACGTCCAGCCCCGAGGGCTGCCGGATCTCCCATGGACATACGTATGACGCCAGCAACCTGGACCAGGTCCTGTTCCCCTACCCAGAGGACAATG IRF4 pro-c401573 NO:86 GCCAGAGGAAAAACATTGAGAAGCTGCTGAGCCACCTGGAGAGGGCGTGGTCCTCTGGATGGCCCCCGACGGGCTCTATGCGAAAAGACTGTGCCAGAGCAGGATCTACTGGGACGGGCCCCTGGCGCTGTGCAACGACCGGCCCAACA IRF4 pro-c407454 NO:87 GTAGAACCTCTGCTAGCCAGACAACTATATTTGCTCAACAAAACAGTGGACATTTCCTGAGGGGCTACGATTTACCAGAACACATCAGCAATCCAGAAGATTACCAGATCTATCCGCCATTCCTCTATTCAAGAATGAAAAATG DEC pro-c18224090 NO:88 TACTGTAGTGGTGATACATCCATTTAATAAGTGTGCGTACTCATCAAGAACTTTCATACAGAACAACTCCATTTCCTCACTTCCAAATACTATGCATGACTACTTACCTTCTTTATAGCAAATGGTCATAAAATTAACCTTAATTATT DEC pro-c18224240 NO:89 CAGTTTTGTGAAGTCACTCCAAGAAAATGGTCCATTACCAAAAGAGTTCCAAATTGTATAGGTTATAGGCTGAATTTAAAAATCCCTTTCAGAATTATTCCCCATAAGGAAATGCTATATACACCTATAAGATATCAAAT DEC pro-c18224390 NO:90 GCAAGTGACTTAAAACACCAGTGATTTATTTTTATTGACAAGGTTTATAAGAACAAATATTTAAAATCGAAGGCCAATTATTAGGTCTCATTTAGTTGCTTATTTTGTTCACTTGTATTTACCTTTCCCTAGTGTCTGAGTAAC DEC pro-c18224540 NO:91 TATCAAGAAACAAACCTGTGAAAATACCTGTTAACATTCCAACATATTTTTTTATTTCTGTTCTATGATGCAAAGATATTTCAACACTTAATGGTGCAACAAATGTGTCATTGTGTCATAAACAGTTTTAAAATTCAGAT DEC pro-c18224690 NO:92 TTAATAAACTGATTTAAGACAGTAATTTGAAAGACAAAATTAAGTCTCATTCAGGAGTGGTCCATTATGTTGATCATCTAGAATCAACACTGATTAACCAAACTCTGAAAGCCAAGAGCCCCAACTCCAGAAACATTAATTTCTTT CCND3 pro-c41902670 NO:93 TCCAAGAAGCCAAAGCCAGTTTTATTCCAATCATCTTTTACAGTAGGATGGTCCTGGGCCACCAGCCTAAACCTTGCAGCCTTAGGAAAGACCTGTCAACAGGGCTTGCCTCCCTCTCTAGACAGGGGCTCAGCCACCTCC CCND3 pro-c41902820 NO:94 AGGGCATCCCTGCTGCATTTTTCCCTGCTGGAGGATGGGGCAGAGGGACAATGGGAGAGGAGGGCATGACCCCACCAGGCTATAGCAGCTCCTTGGCCACAAAGATCCTTTTGCCAGTAGCAGAAGGGAGGAAAACAGCAACCACCAGG CCND3 pro-c41902970 NO:95 GTTACCACCACTTGTGGGATGGCCAGGACCCATTATGTCCTCTTAAAGTTGTGCTCAAAGCAATTAAAATTAAAATGAGCCTTCAGCAGCAAAGCTGTCAATCACACAGAGAAGCTGAGCAGAAAGCAAAGCAAAGGAATTTAT CCND3 pro-c41903120 NO:96 AGCTTCTCTGGCTGAGGCCTAGGCCCCTCCCTCTAGGAGCAGCTGTCAGCACGGACTACATAGGGGCCTCCAAAGTACTGAGAGGAGCCATCTAGACTATTCCCCCTCATATGTGTCTATCATGCATTAAATTTTAGAGGGACCCCAATC CCND3 pro-c41903270 NO:97 CAAATGCAATAACCCTAGAAGGGACAACACCTTTAGAAGGCACTAGAGCATTTTGGCAGTTGAAAACATGAGAGCCCCCAGGGGTGGGGGGGGGCGTTCAAAAGGAATGCTGGTGTATGTATCCAATTCTGTCCCATCAGCCTGGCCCAC AFDN pro-c168227243 NO:98 ACTCTGCACCGCGCGTCCGGACCCAACACAGCACCACCCATCGGACCCAGCACTGCCCATTTAGATCCAGCAGCGCGCGTCCGAACCGAACCTAGCACCGCTGGTCGGGAAGGAACCCGGTACCGCCGGTTGGGACCCACCACCGCCAGT AFDN pro-c168227393 NO:99 CCAGACGGAACCCTAGCACCGCGCGGGGCCCGCCCTCCGACACCTCCCTCCCTGGCGGCCGCGGCAGCAGCACCTAGTGGAGCGGCCCGGAGGTGCGGCGGCGCCGCGCGGGGCGGCGGCGCGCACGGCGGGCGGCGGCGCGCACGGCGG AFDN pro-c168227543 NO:100 CGGGCGGGGCGCGGGCGGGTGCGGGCGGCGGGCGGCGGGCGCCGGGCGCACACCGGCCGGCGGGGCTGAGGCGGCGCGGCGCGGCGCGGACTGAGCCCCAGGCGGAGGCCAAGTCGGAGGACGCGGCGCGGCCGCGGAGGCGGAGGCAGC AFDN pro-c168227693 NO:101 CGCGGAGGCGGAGGCGGCCGGCGGGGGGTGGCGAGGGGCGCCGGGCCCCCGCGGACCTGTCGTCCTCGGCCCGTCCTCCGGCCCCGGCCCCGCGCGGCTGAGGAGGCGCGGCCAGGACCATGTCGGCGGGCGGCCGTGACGAGGAGCGGC AFDN pro-c168265230 NO:102 GATTTGGAGTTCCATGGAGTGATGAGATTTTATTTTCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACTGCCACCACTCAAGATGTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATG HOXA13 pro-c27233982 NO:103 TTTTTTTTTAAAGTCCCATTTTTATTTTCTTAATTCAGGAAGAGTTTTCTTTTTAGAAAAAAATACTTTAATCAGGCTTTCAACAACATTATCCATGGGTCAGTGGCTGATACTATTATTCCTATTTTTCAGGAGGTGGCTGGTCTCTCC HOXA13 pro-c27234132 NO:104 TTGATTTTTGTTTTTGTTTTTGTTTTTGTTTTAAGGTTTTAGACTGATTGCTATTTGGGCATTAAAGGAGCCATAATAAATAATCCATGCCCACTTTAGGTTATCTGGTAGATCCACAGAAATTTTAAATAGGAGGAGAGTTAGGTAAGA HOXA13 pro-c27234282 NO:105 TCGACACTATCAATGACCATTTTAGAACTGGGGGGAAAAAATCCCCACAACAACCCTGAAATGTCTTCTGTCATTACAGTTTCAAAAACTAGAGAGAGAAAAAAAGAAGGCTACTACTTTACCCAGGGTTCCTGTAGTGGTGATGGCTTT HOXA13 pro-c27234432 NO:106 CGAAAGGGGCGGGATCCCGGCTGGAGAGCTGCTGTTGGCCTCCTTCCTAGGCTCGAGGCTCAGAATATTTCTTACATCTAAAGAAAAATATCCCCTGTCAACAGAAGAGTCCCTTTTGGAGCTGTTCTTAAACACACAGTTTGATCCAGC HOXA13 pro-c27234582 NO:107 TTTGAGGGGATTTTCCACCACTTTAAACATTTTGGGAGAAAGTTGTTACTTTGGCTTGATGGCAGCTCATTTGGAAATGGAGTACTGTTTGGAACAAGAGGTGGAGAGGTGGGTCTGAAGCAACATTATCATTTGTTTCCACAAGTGGAG MNX1 pro-c156797547 NO:108 TCCAGTACAAAACCAATTTATTTTACATTCCATCGTGGGCGATGGGAGAAGCTAAATAATAATTTAGCATGCATACGTAGACGTTTCTTTTTATATATAAATACAATATACAAAAATAAAGACAGTACAGTTTTGAGATTTCCAGCAGTT MNX1 pro-c156797697 NO:109 TGAACGCTCGTGACATAATCCCCCCGACCCCAGAGACGTAAGCATAAACCCTTTTTTCTTTTTCCAAATACTCTGCAGAATGGCGGCTCCAGAGGCGGTTTCAAGTTTCATAAGTCAGGTAACACTGTGGGTTTCCGCCTTCTCGGACGC MNX1 pro-c156797847 NO:110 GGGGAAAGGGGAGACAGGAGGCTTCCCCTTGCGCGGGGTGGGTCGGTTCTTCTCACACGCACTCGCGCCTCCCCCAACACCAACCAAAATTAATTTTAAAAGAACCAGAGTTCAAGTTTCAGCCCCCTGGGTCTCCCTCTCGCTGTTTCT MNX1 pro-c156797997 NO:111 TGAAGAGCAGGTGAGGCGCCCTTGCTTAAAAGGGAAGCGCCCAGGACCGGAGCCGCGGCCGAATCCCTCCAGCCCCGGCCTGGGCGAGGGGTCCATGGGGCGGCGGTCGAGCCTGCTCTCGGGGCCGGGCCGGGTGGGTGCGGGCGCCGG MNX1 pro-c156798147 NO:112 GGCCTCCGGGAGAAGCCGCCGGCCGGGGCGCTCCGTGCGCGCCGCACCTGCTGGGCCGCGGGGCTCCTACTGGGGCGCGGGCTGGTGGCTGGGCCGCGGGGGCGGCGAGTCGTCCTCCGAGGAGCAGTCGGAGGAGGCGGCGTGGACGCT PCM1 pro-c17780496 NO:113 ACAATGAGATCCTAAGATGGCGGTGGCTGCGGCGGTTGGCGCTGCGTAGCTGAGGTCGAAAAGGCGGCCACTGGGGCCGAGGCAGCCAGGAAACGTGTGGGCCTCTCTGCTGCGGTCTCCGAGGGCCGACCGCTGCCGGCGGCGGGTCGT PCM1 pro-c17794642 NO:114 GATTGGGGTGCCCAACAGAAGAAAGCAAATAGATCATCAGAAAAGAATAAGAAAAAGTTTGGTGTAGAAAGTGATAAAAGAGTAACCAATGATATTTCTCCGGAGTCGTCACCAGGAGTTGGAAGGCGAAGAACAAAGACTCCACATACG PCM1 pro-c17796248 NO:115 AGAAGCATTGGAAGTGATTCCCAAGGTAGAGCAACAGCTGCTAACAACAAACGTCAGCTTAGTGAAAACCGAAAGCCCTTCAACTTTTTGCCTATGCAGATTAATACTAACAAGAGCAAAGATGCATCTACAAACCCCCCAAACAGAGAA PCM1 pro-c17797199 NO:116 ATTGTAAGCAGGCTTGTTCAAATTCGCGATTATATTACTAAAGCTAGTTCCATGCGGGAAGATCTTGTAGAGAAAAATGAGAGATCTGCTAATGTTGAGCGCCTTACTCATCTAATAGATCACCTTTAAAGAACAAGAGAAGTCATATATG PCM1 pro-c17804694 NO:117 GCCAGAGATCCCTCAGCAGGAGCCTATGGAAGAGATAGAAAATTTGAAGAAACAACATGATTTATTAAAAAGAATGTTACAACAGCAGGAGCAACTAAGAGCTCTACAGGGACGGCAGGCTGCACTTCTAGCTCTGCAACATAAAGCAGAG RUNX1T1 pro-c92967194 NO:118 CATGATTAGGCAAACACAACTTTATTTATTCACCTTGGAATAAGGGTATAGACACCCATACAGAACAGCACTCCTTTGCTCATTTCTTCCCCATCCATTCCAGGGAAATAGCTCAATGTTCTTTCACGGGAGACACATACTTTTATAATG RUNX1T1 pro-c92967344 NO:119 TTATCAAATACATCATCAACACTTCTAAAAAAATGGCGTCCACCACCTGGAAGTCTCATACTGTTCGCGTAAACTAAAACTGTTTCCTATTTGTCCAACATATCCACTTGAATGAAAACGTCACAGAGGTGTTTGCAAGAACTTCTTATA RUNX1T1 pro-c92967494 NO:120 AGGGAACTAAGTCAACGGGGTTCCATTTCTATCTGCTAGTTTCTGGCCTTTTTCACTTTGAAGAGAAAATAAGATGCAGTAACACATTAAAAATAGGCAACTTGGGTCAGGCTGGCCTTTCTTCTGTGGAAAAAAAGAGTAACTATGAAAT RUNX1T1 pro-c92967644 NO:121 TAAATATCCAACCCTAACTGTCCAAAGTGTGGGAGTGAGGGTGTTTCCACTATTTAAAAAAAAATCTGTTCTGATACCTAGTTTTTCCTGGTGTGCACTACCAAATTCTTGGAAAAGCTACATTTCCCAGGAAGACAAGTTAGAGAAGCC RUNX1T1 pro-c92967794 NO:122 TGGCCTTGAAACAGGAACCCAGCTGGCACAAGAAGGAAACTAGAGGGCTCCAAGTCACCCTCCCATGTAACCAGCATTCACCTTCATAACCTTTCTCCAGACACGGGCTCATGCACCTGCTGTGCCATCCTTGATTTTTTGCAACGATTA MYC pro-c128747679 NO:123 GGAGTTTATTCATAACGCGCTCTCCAAGTATACGTGGCAATGCGTTGCTGGGTTATTTTAATCATTCTAGGCATCGTTTTCCTCCTTATGCCTCTATCATTCCTCCCTATCTACACTAACATCCCACGCTCTGAACGCGCGCCCATTAAT MYC pro-c128747829 NO:124 ACCCTTCTTTCCTCCACTCTCCCTGGGACTCTTGATCAAAGCGCGGCCCTTTCCCCAGCCTTAGCGAGGCGCCCTGCAGCCTGGTACGCGCGTGGCGTGGCGGTGGGCGCGCAGTGCGTTCTCGGTGTGGAGGGCAGCTGTTCCGCCTGC MYC pro-c128747979 NO:125 GATGATTTATACTCACAGGACAAGGATGCGGTTTGTCAAACAGTACTGCTACGGAGGAGCAGCAGAGAAAGGGAGAGGGTTTGAGAGGGAGCAAAAGAAAATGGTAGGCGCGCGTAGTTAATTCATGCGGCTCTCTTACTCTGTTTACAT MYC pro-c128748129 NO:126 CCTAGAGCTAGAGTGCTCGGCTGCCCGGCTGAGTCTCCTCCCCACCTTCCCCACCCTCCCCACCCTCCCCATAAGCGCCCCTCCCGGGTTCCCAAAGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCTCTCTCGCTAATCTCCGCCCAC MYC pro-c128748279 NO:127 CGGCCCTTTATAATGCGAGGGTCTGGACGGCTGAGGACCCCCGAGCTGTGCTGCTCGCGGCCGCCACCGCCGGGCCCCGGCCGTCCCTGGCTCCCCTCCTGCCTCGAGAAGGGCAGGGCTTCTCAGAGGCTTGGCGGGAAAAAGAACGGA JAK2 pro-c5021962 NO:128 GCAAATGTTCTGAAAAAGACTCTGCATGGGAATGGCCTGCCTTACGATGACAGAAATGGAGGGAACATCCACCTCTTCTATATATCAGAATGGTGATATTTCTGGAAATGCCAATTCTATGAAGCAAATAGATCCAGTTCTTCAGGTGTA JAK2 pro-c5054562 NO:129 CTACAAGACATTCTTACCAAAATGTATTCGAGCAAAGATCCAAGACTATCATATTTTGACAAGGAAGCGAATAAGGTACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCCAGAAACTTGAAACTTAAGTATCT JAK2 pro-c5054712 NO:130 TATAAATCTGGAAACTCTGCAGTCTGCCTTCTACACAGAGAAATTTGAAGTAAAAGAACCTGGAAGTGGTCCTTCAGGTGAGGAGATTTTTGCAACCATTATAATAACTGGAAACGGTGGAATTCAGTGGTCAAGAGGGAAACATAAAGA JAK2 pro-c5064882 NO:131 GAAATTGAACTTAGCTCATTAAGGGAAGCTTTGTCTTTCGTGTCATTAATTGATGGATATTATAGATTAACTGCAGATGCACATCATTACCTCTGTAAAGAAGTAGCACCTCCAGCCGTGCTTGAAAATATACAAAGCAACTGTCATGGC JAK2 pro-c5069021 NO:132 CGAGAAAATGTCATTGAATATAAACACTGTTTGATTACAAAAATGAGAATGAAGAGTACAACCTCAGTGGGACAAAGAAGAACTTCAGCAGTCTTAAAGATCTTTTGAATTGTTACCAGATGGAAACTGTTCGCTCAGACAATATAATT MLLT3 pro-c20341666 NO:133 TTATTTACCAAACTGAATGTATTTTATTCTCTTTGTTGTAGTAGTTGTTCTCCTTCCTGTCTAATCAAGGTAGCAGTTCTTGCTGTGAAAGTAAATGCAGGGACTTTGTAAGATCGACTCTCTCTGGCTATAGCACATTGTTTCTGAACA MLLT3 pro-c20341816 NO:134 AAACCCTCCAAAGAAATAGGGGGGAAAGAAAGATACCGGGCACACATAGCCCAGTTTATGCCAAAAAATGTATCCCACCACTTCTTGTTCCAATCTTTGCAGAGTTTTAAAAATATTATATATATACTGGCTTTAGGACACAGAAGATG MLLT3 pro-c20341966 NO:135 GTGTTAGACATGAGGGACTCCTAAAATGAATGAGGCATGGACTCTCTGCCTTCAGCTGGTTTTGGACACATTGTCCTCTCTCTGGATGTCTCAGATCTCCCCATCTATTCTTCTTTAGAAACAGCATCACTTCTTGAGAAAAGGCTAGGG MLLT3 pro-c20342116 NO:136 GCTAATTTTTCCTGTATGCAAAATAAAGCCAAACTTTGGTCAAATGACTCTCAGCAAATCAGTACAATTATACATTTTAAAAAAGGTGCTGAAACAGATTATATGAGTTTAAGTAAAACACCTATTTCTATCAATCTGTACTTACATTTC MLLT3 pro-c20342266 NO:137 AACATGGATTTTAGAGAAGGGAAATACATCTTACAGTGTGCCTTGAATTCACACAAAAGCATGTACACATACACAATGTATCTTTCAACATAACTACACATACACAGTCCATTAAAAAGATACTGACAGATTTATAAAATGTATTGGCCT ABL1 pro-c133588429 NO:138 AATCCATCATGGCGGCCGCGGGGTTTGGTGTCTGTGCCTGAGCAGCGCTGGAGCCGGAGCCGGTTCCCGGGTCCTGCGGCTGAGGAGCCCCTCCGCTGTCCACGGCCCCTACCGGCGGGGGGCGGCTGGGTCCCTCGGCGGAGCTCGGGA ABL1 pro-c133588579 NO:139 GATGTGACTGCCTGAGGGCGGTGGTGGTGTCAGCGTCCGGGGCCGGGGGAGGGGGTGTCTCGGGCAGAGACCCCCGGGCTTGGGGCAGCTGAGGCGGCCGGGCCTCCTCTACACGGGGCCCGCCTTCCGCTGTCTGGGCCGCGAGAGTCC ABL1 pro-c133588729 NO:140 TTCGTCCCTTACAGCCCCGCCCCGGCTTTGGGACACTGCGGGTGGTCTGTTTCCCCCAGCTTGGGACACCCCGTTTTCTGAGGCGTGGAAGAGCGTCGCCCCGGAGTAAGCTGCCCGTGCCGCGCCCCGACAGCTTCCCTCAGCCCCAAG ABL1 pro-c133588879 NO:141 CCGCCCCTTATTCCGGATCCCGGCCCCAACTTTGGCCACGGAGCCTCCCATTCAAATCCCTCCCTTGCTGTCAAGGGGTCTCCCCTTCCCCCAAGGTGGCTCCCGCGAGCCTCTAATGCCCTGACTTCTTCCAATGTCACCTACGGCCCC ABL1 pro-c133589029 NO:142 CTTAGTCTCAGCTCAGCCAAAAACTTTAATGCAAAGGAAAAGTCTGGATTGGTTCCACAGGCCTTTTAAAAAGCGGACTTAAAAGTTGCTGGCAATGCATTCCTTTTCGTCAGAGTCGAGGGCAAACTCGCTGAAATCTGGGTGACCCGT NUP214 pro-c134000972 NO:143 GAGGTCAACTGCGCGCCGCTGGCGCTGAGGGGAGGAAGTTTGCTGTCGAGCGGCCTGGGTTCCGTGGGCAAGGCCGTGGGAGGCAGCGTTGGCTGCTTCGACACACTGAGGGCGGCGCGATGGGAGACGAGATGGATGCCATGATTCCCG NUP214 pro-c134002910 NO:144 GATTTTCAGTTTAGAGCGCTAAAGAAGGTGAGAATCTTTGACTCCCCTGAGGAATTGCCCAAGGAACGCTCGAGTCTGCTTGCTGTGTCCAACAAATATGGTCTGGTCTTCGCTGGTGGAGCCAGTGGCTTGCAGATTTTTCCTACTAAA NUP214 pro-c134003718 NO:145 TTGATAAAGTCCAAGGCTTGCTAGTTCCTATGAAATTCCCAATCCATCACCTGGCCTTGAGCTGTGATAACCTCACACTCTCTGCGTGCATGATGTCCAGTGAATATGGTTCCATTATTGCTTTTTTTGATGTTCGCACATTCTCAAATG NUP214 pro-c134004665 NO:146 GCTAAACAGCAAAAACGCCCATTTGCCTATCATAAGCTTTTGAAAGATGCAGGAGGCATGGTGATTGATATGAAGTGGAACCCCACTGTCCCCTCCATGGTGGCAGTTTGTCTGGCTGATGGTAGTATTGCTGTCCTGCAAGTCACGGAA NUP214 pro-c134015935 NO:147 GTGATGAAAAGACTCTTCCTCCTGCTCCAGTTCTCATGTTACTTTCAACAGATGGTGTGCTTTGTCCATTTTATATGATTAATCAAAATCCTGGGGTTAAGTCTCTCATCAAAACACCAGAGCGACTTTCATTAGAAGGAGAGCGACAGC MLLT10 pro-c21823573 NO:148 ATGGTCTCTAGCGACCGGCCCGTGTCACTGGAGGACGAGGTCTCCCATAGTATGAAGGAGATGATTGGAGGCTGTTGCGTTTGCTCAGACGAGAGAGGCTGGGCCGAGAACCCGCTGGTTTATTGCGACGGGCACGGCTGCAGCGTCGCG MLLT10 pro-c21845571 NO:149 ATGGTATGCAATTCCTGTTGGTTAGCCTCATCTGAAAACGTCACTCCTGGATACATAGAACATCACTGCGCATGTGCATCTCCCCACCCCCGATGCCTGGTGTCTAACGTTCCTCCAGTTTCTGGTGCTCTGATGCATTGCTTTTGGGCT MLLT10 pro-c21845721 NO:150 TGTCTTACTACAGCAGCTTTCTTCGGTCCTCAGTCATTTACCACTTGTCATATGTCCTTTCTAGTTTCCAGGGATATTCTTTTTTATATTTATGGCTTTATGCCATTTATCTCAGTTGTCATTTGGCGTTTCAAGAAGGAGAGGTGGTAG MLLT10 pro-c21845871 NO:151 TGTTTAAGTGGAATTAACTACTGTGTTTCTGATGTGGTCTTTTATTTTTCAGGGTTTTGTTTTACCAAGTCTTCAGTCTATCCTGTTTACTTGATTTTCCCACTCAACTCACAAACATTTGAATCATCTCATGAA NUP98 pro-c3696248 NO:152 TGCTATAAACATTTTATTTTTTAAAAGTAGCTTCAGAAAAAGGAAAATATCTAGATCATTTATACATATATATTTTTAATAAAACTTTACATTATCTTCATGCATAAAGACCCAGGTGTGGCCATATTGCCTG NUP98 pro-c3696398 NO:153 AGATAGCCCTAACACCAGCTATCTCATGCCATTACCTGGGGCTCCCTATAGCTGTCAGCAGTGTAGCAAAGGCCCTCTGCCTGCCCCTAACGCCCAACTCCATGCCTGCCTCAGTCATGGTTTGTTTTTGCTTCTGGAAGGGGTAGATGG NUP98 pro-c3696548 NO:154 AGGGTGGGAAATGCTTTGGAGCTAACATGGGATGAACAGGGAGGGTTACCCAACCTGCCATTTTTTATCCAAACTCTGCAGGCAAATCCAGACAGAGTCTCAGCAAGACTGAAACAGGGACCAAACCAAACTCTGGGAGCTTCCCACA NUP98 pro-c3696698 NO:155 GACATGTCACATGTGACCAAGGGTTCCTGCACATAGTAGTAGCAAAGGTATGGTGTTCATGGAACTCTAAAGGCAGGAACAAAAACAGCCCTGAAGATCAATCCCTCCGTGACAGGCATTCACTTCTGCCCTAAGTGTGTCAGAAAGATC NUP98 pro-c3696848 NO:156 CCATTTTGTTTCCTAACTCAGGCCAACCCTCCTTTGCCTCCAAAATTCAGTGTTAACTAACCAATTACTTAAGAGACGGATCCCTCTTCCTTCTGTGGATAGTTCATCTGTTAAGGATCCATTATCACCTGTCTCACTCCTCCTAAGGAG CCND1 pro-c69455923 NO:157 AGAGGGCTGTCGGCGCAGTAGCAGCGAGCAGCAGAGTCCGCACGCTCCGGCGAGGGGCAGAAGAGCGCGAGGGAGCGCGGGGCAGCAGAAGCGAGAGCCGAGCGCGGACCCAGCCAGGACCCACAGCCCTCCCCAGCTGCCCAGGAAGAG CCND1 pro-c69456073 NO:158 CCCCAGCCATGGAACACCAGCTCCTGTGCTGCGAAGTGGAAACCATCCGCCGCGCGTACCCCGATGCCAACCTCCTCAACGACCGGGTGCTGCGGGCCATGCTGAAGGCGGAGGAGACCTGCGCGCCCTCGGTGTCCTACTTCAAATGTG CCND1 pro-c69457798 NO:159 GTCTGCGAGGAACAGAAGTGCGAGGAGGAGGTCTTCCCGCTGGCCATGAACTACCTGGACCGCTTCCTGTCGCTGGAGCCCGTGAAAAAGAGCCGCCTGCAGCTGCTGGGGGCCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACC CCND1 pro-c69458599 NO:160 CAAATGGAGCTGCTCCTGGTGAACAAGCTCAAGTGGAACCTGGCCGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAATGCCAGAGGCGGAGGAGAACAAACAGATCATCCGCAAACACGCGCAGACCTTCGTTGCCCTC CCND1 pro-c69462761 NO:161 ATGTGAAGTTCATTTCCAATCCGCCCTCCATGGTGGCAGCGGGGAGCGTGGTGGCCGCAGTGCAAGGCCTGAACCTGAGGAGGCCCCAACAACTTCCTGTCCTACTACCGCCTCACACGCTTCCTCTCCAGAGTGATCAAGTGTGACCCGG BIRC3 pro-c102192567 NO:162 GTTTACAAAGGAGGAAAACGACTTCTTCTAGATTTTTTTTTCAGTTTCTTCTATAAATCAAAACATCTCAAAATGGAGACCTAAAATCCTTAAAGGGACTTAGTCTAATCTCGGGAGGTAGTTTTGTGCATGGGTAAACAAATTAAGTAT BIRC3 pro-c102192717 NO:163 TAACTGGTGTTTTACTATCCAAAGAATGCTAATTTTATAAACATGATCGAGTTATATAAGGTATACCATAATGAGTTTGATTTTGAATTTGATTTGTGGAAATAAAGGAAAAGTGATTCTAGCTGGGGCATATTGTTAAAGCATTTTTTT BIRC3 pro-c102192867 NO:164 CAGAGTTGGCCAGGCAGTCTCCTACTGGCACATTCTCCCATTATGTAGAATAGAAATAGTACCTGTGTTTGGGAAAGATTTTAAAATGAGTGACAGTTATTTGGAACAAAGAGCTAATAATCAATCCACTGCAAATTAAAGAAACATGCA BIRC3 pro-c102193017 NO:165 GATGAAAGTTTTGACACATTAAAATACTTCTACAGTGACAAAGAAAAATCAAGAACAAAGCTTTTTGATATGTGCAACAAATTTAGAGGAAGTAAAAAGATAAATGTGATGATTGGTCAAGAAAATTATCCAGTTATTTACAAGGCCACTG BIRC3 pro-c102193167 NO:166 ATATTTTAAACGTCCAAAAGTTTGTTTAAATGGGCTGTTACCGCTGAGAATGATGAGGATGAGAATGATGGTTGAAGGTTACATTTTAGGAAATGAAGAAACTTAGAAAATTAATATAAAGACAGTGATGAATACAAAGAAGATTTTTAT KMT2A pro-c118307206 NO:167 GCTTCACTTCACGGGGCGAACATGGCGCACAGCTGTCGGTGGCGCTTCCCCGCCCGACCCGGGACCACCGGGGGCGGCGGCGGCGGGGGGCGCCGGGGCCTAGGGGGCGCCCCGCGGCAACGCGTCCCGGCCCTGCTGCTTCCCCCCGGG KMT2A pro-c118307356 NO:168 CCCCCGGTCGGCGGTGGCGGCCCCGGGGCGCCCCCCTCCCCCCCGGCTGTGGCGGCCGCGGCGGCGGCGGCGGGAAGCAGCGGGGCTGGGGTTCCAGGGGGAGCGGCCGCCGCCTCAGCAGCCTCCTCGTCGTCCGCCTCGTCTTCGTCT KMT2A pro-c118307506 NO:169 TCGTCATCGTCCTCAGCCTCTTCAGGGCCGGCCCTGCTCCGGGTGGGCCCGGGCTTCGACGCGGCGCTGCAGGTCTCGGCCGCCATCGGCACCAACCTGCGCCGGTTCCGGGCCGTGTTTGGGGAGAGCGGCGGGGGAGGCGGCAGCGGA KMT2A pro-c118342376 NO:170 TTAAAACTAGTCCTCGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTATCCTCTCAGATCCATCTGTGTTTTCCCCTCTAAATAAATCAGAGACCAAATCTGGAGATAAGATCAAGAAGAAAGATTCTAAAAGTA KMT2A pro-c118342526 NO:171 TAGAAAAGAAGAGAGGAAGACCTCCCACCTTCCCTGGAGTAAAAATCAAAATAACACATGGAAAGGACATTTCAGAGTTACCAAAGGGAAACAAAGAAGATAGCCTGAAAAAAATTAAAAGGACACCTTCTGCTACGTTTCAGCAAGCCA ETV6 pro-c11802607 NO:172 ACGTCAGTTTCTGCACTGAAACTCTCAAGATCAATGAGCAAAGAGCTTTCTCAGTTCTGCCTTTCAGTTTCTCTCTTCCAGGAAGGAAAACATTCGAGAGAGCGAGGGAGAGCCGCGGGAGGGCGGGGGGCGGGGGCGCCGGCTGCGGGT ETV6 pro-c11802757 NO:173 GGGAGGAGAGACCGGGAGGCCGGCCGGGCTGCGTCCCGGGTCCCCGCGCCGCGCCGCGACCTGCAGACCCCGCCGCCGCGCTCGGGCCCGTCTCCCACGCCCCCGCCGCCCCGCGCGCCCAACTCCGCCGGCCGCCCCGCCCCGCCCCGC ETV6 pro-c11802907 NO:174 GCGCTCCAGACCCCCGGGGCGGCTGCCGGGAGAGATGCTGGAAGAAACTTCTTAAATGACCGCGTCTGGCTGGCCGTGGAGCCTTTCTGGGTTGGGGAGAGGAAAGGAAAGTGGAAAAAACCTGAGAACTTCCTGATCTCTCTCGCTGTG ETV6 pro-c11992073 NO:175 GCTTGCAGCCAATTTACTGGAGCAGGGATGACGTAGCCCAGTGGCTCAAGTGGGCTGAAAATGAGTTTTCTTTAAGGCCAATTGACAGCAACACGTTTGAAATGAATGGCAAAGCTCTCCTGCTGCTGACCAAAGAGGACTTTCGCTATC ETV6 pro-c12022357 NO:176 ATAACTGTGTCCAGAGGACCCCCAGGCCATCCGTGGATAATGTGCACCATAACCCTCCCACCATTGAACTGTTGCACCGCTCCAGGTCACCTATCACGACAAATCACCGGCCTTCTCCTGACCCCGAGCAGCGGCCCCTCCGGTCCCCCC BCL11B pro-c99635623 NO:177 TTTGGAGATCAAGTTAAATGTCCAATTTATTTTTATAACTTGAAACCAGAACAAACTTGGTTTTGAACAAGCGTACAAATGAAATGGCAGACACATGCTGAACTCAACATTGTGACATTAAGGAAAAAAAGAGGCCCAAAATCTTGTACA BCL11B pro-c99635773 NO:178 GAGAATAAAGGAACAATAAATATTTTACTAAGCCATATTGAAATGACCTCCTGTCATCTCAACATTTTATCCATAATAAAAAAAAGTGCCTGTTTCTTAAACAGAGCACAAATACCAAGCAATAATAAATAGTTCCAAACTTGTAGTAAA BCL11B pro-c99635923 NO:179 AGACAAAACCTCCAAGTAAACAACAAAAGCTCATACAATAAGTAATAGAAAAGGTAATAAAAATATTTTGCCTTGCCAGTACAAATGCAAACAACTTAAATCAATGGGACTTTGCTTTGCAGGGCTGAGTTACAAGGAGCACCAAAATCA BCL11B pro-c99636073 NO:180 CGGGTTTGCCTGTGTTCCACGAGACCTTCGGCTGACGGTTACTTAGGACCGGGATGAAAGGCTGATTTCCTTACTTTTCACATTTTGCTTAGAGTAATTCAGTCTCCTTCTCTCCCCATGAAAGACCCTCTAATGCCAAGTGGCAGGTTC BCL11B pro-c99636223 NO:181 CAATTGGGGGCAACTTTGAACAAAGTTGTGGCAGTGCGGAAACCGGCCTGAGGTCGGCTGGGTCACCCATGCTAGAGGCCTCTCTCTCGGGATCATCTGCTTCCGTGTTGGTTTTTTAAACACAAAACAGAAGCAAAATTCTCTCTTCTC PML pro-c74287056 NO:182 AGCTCAAGGGACTCAGCCAACTGGCTCACGCCTCCCCTTCAGCTTCTCTTCACGCACTCCAAGATCTAAACCGAGAATCGAAACTAAGCTGGGGTCCATGGAGCCTGCACCCGCCCGATCTCCGAGGCCCCAGCAGGACCCCGCCCGGCC PML pro-c74290344 NO:183 CGAGCCCCCGCTTCGGAGGAGGAGTTCCAGTTTCTGCGCTGCCAGCAATGCCAGGCGGAAGCCAAGTGCCCGAAGCTGCTGCCTTGTCTGCACACGCTGTGCTCAGGATGCCTGGAGGCGTCGGGCATGCAGTGCCCCATCTGCCAGGCG PML pro-c74290494 NO:184 CCCTGGCCCCTAGGTGCAGACACACCCGCCCTGGATAACGTCTTTTTCGAGAGTCTGCAGCGGCGCCTGTCGGTGTACCGGCAGATTGTGGATGCGCAGGCTGTGTGCACCCGCTGCAAAGAGTCGGCCGACTTCTGGTGCTTTGAGTGC PML pro-c74290644 NO:185 GAGCAGCTCCTCTGCGCCAAGTGCTTCGAGGCACACCAGTGGTTCCTCAAGCACGAGGCCCGGCCCCTAGCAGAGCTGCGCAACCAGTCGGTGCGTGAGTTCCTGGACGGCACCCGCAAGACCAACAACATCTTCTGCTCCAACCCCAAC PML pro-c74315168 NO:186 CATCTACTGCCGAGGATGTTCCAAGCCGCTGTGCTGCTCGTGCGCGCTCCTTGACAGCAGCCACAGTGAGCTCAAGTGCGACATCAGCGCAGAGATCCAGCAGCGACAGGAGGAGCTGGACGCCATGACGCAGGCGCTGCAGGAGCAGGA MYH11 pro-c15796991 NO:187 TTGTGATTTATAACCATTTATTAGTGAAAGTGTTTTTAAGCACAGTCAGGGTGTAAACAGTGCAGCATTCCTGCTCCCCTCCGTGGGAGCAGCGTCTCCTTTTCAATTCATGTGACTACAGAAGGCACTTGGTGAACTGTGCGTGTCTGA MYH11 pro-c15797141 NO:188 GGTGTGGAAACCAGGAGAGGGGGAAAGAATTCTCAAAGGCCTGACGTGAGAAGTTGGAAAGGTTTGCAGGTTAGGGAATGAATTGGGAGTGGGGGCCGGCGGCACCCATTTCGGTGACTTTCTCCCCATTTCATGTAAACAGAATTGCCA MYH11 pro-c15797291 NO:189 GGGACCGGTTACCGTGGATATGTTTTTCTAAAAACTCAGTGTCTGCACAATCCATTGATAGAACTGGAGGATGTGTCTGTGTTTCCTGTTGGGTTTTTCTCATCTCTTACATCATACAAACTTCAATTTTTACCTTGAATACAGGGGTAG MYH11 pro-c15797441 NO:190 TAGGGGTGGTGGTGGTGGTGGTGGTTGAGACAGGGTCTCTGTTGCCCAGGCTGGAGTGCAATGATGCAATTATAGCTCATTGCAGCCTCGAAGTCCTGGGCTGGAGCGTTCTTCCTGGCTCAGCCTCCCTAGTAGCTGGGACCACAGGTG MYH11 pro-c15797591 NO:191 TGTACCACCACGCCCAGCTTATTTTTAATTCTTGTATAGATGAGGTTTTACTACGTTGCCCAGGCTGGAGGGTGGTGGTTTTTATATTGTGTGAGGGTGTCTGTGATATTTGGAATTTGAGAATGGATTTAGACAATGCTAAGT CBFB pro-c67063051 NO:192 GGTGAGCGCTGGGGCTGCGCGGGCGGCAGGCAACGGCTGAGGCGGCGGCGGCGGCGGCGGCGTGGGTTGGGCTCGAGCGGGCGGCGGCCTCAGACTCCCCGGAACGGGAGCCCACGGGGCGGCGCCTGAAACAAAGGGAAGCG CBFB pro-c67063201 NO:193 GGCGTCCGGGCGCCGCGGGTGGGCGGTCAGTCGGTCAGCGCGGAGCCAGCCAGGGTGCCCGCGCAAGCCCCGAGCGCGGCCGGCCGGCGGCCTCAGGGCGGGAAGATGCCGCGCGGTGCCCGACCAGAAGCAAGTTCGAGAA CBFB pro-c67132612 NO:194 GCAAGAAGACAACAAGACCCTAGTCCTGGTTCCAATTTAGGTGGTGGTGATGACCTCAAACTTCGTTAATTAATAGCACAGCAGATGTGTGCTGCCCATCTTACACATTGCTTCTAGTTGGCAGAAATATTGATTAAAAGACCA CBFB pro-c67132762 NO:195 GAAACTGTGATAACTGGAGGTACTACGGTCTATTTCTCAACCTTAGGCAGTAATAGACATCAAACTGCCATGGTTTTGCACTATGATTATAATACCTGCATTTCTAATTTTTTAAGCATGTAGCCAGTAATATTGAAGTTTTTTTT CBFB pro-c67132912 NO:196 CTATGCAAGCTTACCTTGTTGGCATTATTTTAGGGAGTTGAAACTATCAACTGTAAAGCTCCTTTTCTTCCACTTTAATTTAAAAGTTCATGTCATTTAAAAACAAGTCAAGAAATTAAAATTGTATCAGAGGGTTTTCTCTAATCATTT MAF pro-c79627734 NO:197 TGTATTTAACTAACTTTGTATTTTGCCACACATTGTTTTCATTTACAGCTATGTCTGACAAATGAGCACGTTTGACATCACTGATAAATGCAACACCGTCTAGGGCCTACGAGAAAACCAGGGGGCTCGGCTCCGCTGGAGCCTCTGCCC MAF pro-c79627884 NO:198 GTGGATTTGTTTAGGGAAGGGGAGTCGAATATCTATTTTTCTTCTTAGTAAAATTATCTGCCAGAGAAACAATGAAGCATGAAAAAGTACTATTTAGAATGTGCATGCCTATATATGATTTTAAAATGCTAATTGTTGCATTCCGGGAAA MAF pro-c79628034 NO:199 CTTTCCATTATATATATGCATATATATGTATCTTTGTAATTTCAGTGAATTTTTAAAACTAGTATGGCAGGTTGAAAGCAATGCACCTGTTTACTTGCACACACCATAAATCGAAAAGCAGGAGTGCGCTTTCCTACCGGTCTCTATGAA MAF pro-c79628184 NO:200 ACCCCCAGACAAGAGGCATAGTTAACTACTACATTTAATAGCCTTCTTCTCTAACACAGTAATTTTTATTTAAAAAGGAGACTAAACAGAAGTCAGGGGTAGGTGGTTCTCCATGACTGCAAATAATAATAATAATGATGATTTTTTTTA MAF pro-c79632681 NO:201 ATGAAAAACTCGGGAGAGGACGGGTTGTCGCTGCTCGAGCCGTTTTCTCGGAAGCCGCTGCTCACCAACTTCTCGTATTTCTCCTTGTACGCGTCCCTCTCGCGCACCAGCCTGGAGATCTCCTGCTTGAGGTGGTCGACTTGCTGCAGC RARA pro-c38487108 NO:202 CATCACAGGACATGGCCCCCTCAGCCACCTAGCTGGGGCCCATCTAGGAGTGGCATCTTTTTTGGTGCCCTGAAGGCCAGCTCTGGACCTTCCCAGGAAAAGTGCCAGCTCACAGAACTGCTTGACCAAAGGACCGGCTCTTGAGACATC RARA pro-c38487258 NO:203 CCCCAACCCACCTGGCCCCCAGCTAGGGTGGGGGCTCCAGGAGACTGAGATTAGCCTGCCCTCTTTGGACAGCAGCTCCAGGACAGGGCGGGTGGGCTGACCACCCAAACCCCATCTGGGCCCAGGCCCCATGCCCCGAGGAGGGGTGGT RARA pro-c38487408 NO:204 CTGAAGCCCACCAGAGCCCCCTGCCAGACTGTCTGCCTCCCTTCTGACTGTGGCCGCTTGGCATGGCCAGCAACAGCAGCTCCTGCCCGACACCTGGGGGCGGGCACCTCAATGGGTACCCGGTGCCTCCCTACGCCTTCTTCTTCCCCC RARA pro-c38504567 NO:205 CCATTGAGACCCAGAGCAGCAGTTCTGAAGAGATAGTGCCCAGCCCTCCCTCGCCACCCCCTCTACCCCGCATCTACAAGCCTTGCTTTGTCTGTCAGGACAAGTCCTCAGGCTACCACTATGGGGTCAGCGCCTGTGAGGGCTGCAAGG RARA pro-c38508161 NO:206 CTGTGAGAAACGACCGAAACAAGAAGAAGAAGGAGGTGCCCAAGCCCGAGTGCTCTGAGAGCTACACGCTGACGCCGGAGGTGGGGGAGCTCATTGAGAAGGTGCGCAAAGCGCACCAGGAAACCTTCCCTGCCCTCTGCCAGCTGGGCA MALT1 pro-c56338696 NO:207 AAACCTGTCTAATTGGGGCGGGGAGCGGAGCTTCCTCCTCTGAGGGCCGTGCCGCGCTGCCAGATTTGTTCTTCCGCCCCTGCCTCCGCGGCTCGGAGGCGAGCGGAAGGTGCCCCGGGGCCGAGGCCCGTGACGGGGCGGGCGGGAGCC MALT1 pro-c56338846 NO:208 CCGGCAGTCCGGGGTCGCCGGCGAGGGCCATGTCGCTGTTGGGGGACCCGCTACAGGCCCTGCCGCCCTCGGCCGCCCCCACGGGGCCGCTGCTCGCCCCTCCGGCCGGCGCGACCCTCAACCGCCTGCGGGAGCCGCTGCTGCGGAGGC MALT1 pro-c56348401 NO:209 TTGCCTAGACCTGGAGCAGTGTTCTCTTAAGGTACTGGAGCCTGAAGGAAGCCCCAGCCTGTGTCTGCTGAAGTTAATGGGTGAAAAAGGTTGCACAGTCACAGAATTGAGTGATTTCCTGCAGGCTATGGAACACACTGAAGTTCTTCA MALT1 pro-c56367672 NO:210 ATTCCAAATGGAAATACATCAGAGCTTATTTTTAATGCAGTGCATGTAAAAGATGCAGGCTTTTATGTCTGTCGAGTTAATAACAATTTCACCTTTGAATTCAGCCAGTGGTCACAGCTGGATGTTTGCGACATCCCAGAGAGCTTCCAG MALT1 pro-c56376609 NO:211 GAAGTGTTGATGGCGTCTCTGAATCCAAGTTGCAAATCTGTGTTGAACCAACTTCCCAAAAGCTGATGCCAGGCAGCACATTGGTTTTACAGTGTGTTGCTGTTGGAAGCCCTATTCCTCACTACCAGTGGTTCAAAAATGAATTACCAT BCL2 pro-c60790578 NO:212 CAGTATCTCACACTGTACTTTATTTTTCTTCACAATATTAACTAGACAGACAAGGAAAGTTTAATGGCAATGTGACTTTTTCCAACAACACAAACAAAGTGCCATTATAGCTAATGGTGGCCAACTGGAGACTTACTTTACCTTAACCAT BCL2 pro-c60790728 NO:213 GTAAAGTATCCTTACCGTATTTTTTATGTGTACAGTGTTGCAGAATATCAGCCACCTCTTAAAAGTATCAATCTTAAAAAGAGCCATGGAAGGTAAAAGTATGAAAATCTTGATAACAAAAGCTTTCAATACAAAAACACTTATTGTACA BCL2 pro-c60790878 NO:214 CTTATTTTTATTTAAAACAAAAATAACCCCAGTAACTCAAAACAAAAGCAAACCTTGGTTGAAAACTTAAGAAGGTATAATAAACAAAACCACCAAAAGAAAGCTTCCCCAAAAGAAATGCAATCCACTGTCACTCTTGCAAATTCTACC BCL2 pro-c60791028 NO:215 TTGGAGGGAAAAACTTAATGAAATGAGCTATCTGGAGGGCCCACGGCAGATTTTCCAAAAGGTTTAGGTGCATGGATTTACTCAGTATCTACACTACAGTCTTATTTATTAATAGTCTCAGAATTTTCTTGATTGAGCGAGCCTTTCCAT BCL2 pro-c60791178 NO:216 CCTCCACCAGTGTTCCCATCTTCTGTGCTCAGCTTGGTATGCAGAACAACCTTGTTGTTGATAGGATGTTTGCTTGAAGTTATTTTTCTGGGGCAGTCCAGATGAACCGGTACAGTACCATTCATGCTCCATCTGATTTTCTTTGCATCC MLLT1 pro-c6210391 NO:217 TGCTTTCCCATCTGACTTTATTTCACTTTTTTTTTCTATAAAACTCCTCCATAATTTCACAATTTAACAAAAGTTTAAAATCCAGGAAAGAAACAATCCTGTAACTCGTTACAAACACGATTCTCCTTATTCCTTTTATCCAGGAGTTGG MLLT1 pro-c6210541 NO:218 TCTCGGGCCGATGAGTGTCCTAGTTCCTAGTGAGACACGTTCTTTGTAAAAACCCTTATGGGCAAGAAAGGTATTCGATACCATTTGCTTTCCGGCGTCGGTTTACATTTTTGTTCAGGAGAGAGCAAAACACAGAGATTTGCACTGGGG MLLT1 pro-c6210691 NO:219 GCAGGCGGCTTAACAGGAAACACGTGGCAATCACAAAGTGCCAGGCCCCTTTCCTATGGAAGTGTCCCCAGAGCCCTCGTGGGCCCAGCCGCCGGGCAGCTGGGGTGGGGTGGGAGAGCAGCTGGGGATGCTGCCTTGGGAGCCCATGCT MLLT1 pro-c6210841 NO:220 GCCCAGCACGTCCATCAGGTGGTCACGGAGCCAGCCCTGGGCCCAGAGCAGCTCTGGAGGACAACGTGAGGCCTTCCGGCCAGAGGAGGTAACTGAGAACTGGGTTGGTGCTTCCGGAGGCCTTGGAAACGGCAGGAAGGGCCAGACGCC MLLT1 pro-c6210991 NO:221 ACCACCGAGAGCAGGCGGGGGAGCCCGACCCTCCTCTGGCTGAGTGGAAGGCTGCTCGAGTCGCTGTGTGGATTTTGGGGGACTCCTGCGAAGGAGAAAGGCCAGCACCCTGGGCTGAGGTTCCTGTCCGTGACCCCGGCGGCCTCTTGT ELL pro-c18553472 NO:222 TTCAGTTCAGTGAAATTTATTGGAGAATGAAAAAAGTCAGCATTCACCTGTTTAGTGTACAAAAAGGACACTAGATCTTTTAAGAAAATATCAGGAAACTGAAGACGCAGATAGATGCCTTTCAATTGTAACATTTTGGCAAAAGTGGAA ELL pro-c18553622 NO:223 AGTTCTTGTAAACACCAACCCCATGGCTCAAAATAGTTTTTCTCCACAGTACAACATTAAAAGAAAAAAAAATAGTATCAATAAGTTAGACCATATTTAATCAGCTAGAACTTTGTCCACCAACCCCCAAAACACAAAACTTTTCTTAGC ELL pro-c18553772 NO:224 TTCATAATTCCTTAAAAGGAGAACAAATAAACAACAAAAACAACAACAACAACAAAAAGGCAAATTCAACTGACAGCCCCTTGGACTGGTTCCCAGGGCAGAGGGGCGGGCAGTCCCGGGCTGGGACCTCCTGAAATCATCCCACCTCAC ELL pro-c18553922 NO:225 CGGTGTCTGCAGGGCTGCCTGCGGGCGACACAGCAAGGTCCAGCTGACTCTGGAGGCCTCCTGGAGCCTGCTCGGCCCTTCCCCGGCCCGGCCCGGCCCAAAGAGAAAAACAACAACAGCTATTGAAACATGAAGGATCAGTTTCCTCGG ELL pro-c18554072 NO:226 AGCTCTGGGAACCCGGGGGTCCCAAAGGAGAGAGACTCTCGGGCAGGGGTGGGATACACTGTGGGCCCTCCACAGTCAGCTCTTTGGAAGCTGTCAGGAGGCACCCCTCCACCCCCCAGTTTAGAAAAATAGACATCTGTATTTTTGCAT MAFB pro-c39314487 NO:227 TCCTCTAAATAAACACCATTTCCATGGTTTTAATAAGCAAAATAGGAAACCATTTTAATAACCAAAAAACAGAAACCAGTCTGAATAAAACTACAATAGACTAGGTTTTAATATTTTCATATCATAAGCAGGGTTTGAAATTGATCCCTT MAFB pro-c39314637 NO:228 ATTTTACATGAAATAAAAACAATTTCTGTTGCGGCAGGTTTGATTTCAACACAGTTGAATCTGTAAAAACCAAAGCTCGTTTCTGATGCAGGACAAATATCCACAATATTTAAAACTGCAAGCACCATGCGGTTCATACAATCTTGTTAT MAFB pro-c39314787 NO:229 TACTGTTAATTTATCAACTAATACAAACTCAAAAATGCATCCGGCCAGCAGCGCCAGCAATTTCAAATGGGAACTTAAAAATACACTTTTATTTTGGTATTTTTGTCAGTGCAACTTAAATCCTTTTACTGACCTGCAGAAAAAAAAAAG MAFB pro-c39314937 NO:230 TAATAATAAAGAAAAACACCCATATCTTCCCTATAACTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTTCCTACTATCTCAAAAGCAGGGAAAGAAACGCAATGCATTGGTCTAAAGAACGCACTTGAAAGTT MAFB pro-c39315087 NO:231 GCAAAATTACTTGCCAATGTTTGGGTTTCTGGTACATTCTGAGCATAGCAGTTGGTTCAGTGCAGTGTCTGCTTACCAGTGCACTGCCAGGGTCAGGGATGGCTAAGCCTCTCACCCTAGGAGCGCTGTGGCTCCTACAATTAGCGCAGG RUNX1 pro-c36160097 NO:232 TATGGTGTATGTACTTGTCAAACTGTTTATTTGCCATTCACACTGATTGTAACACTGGATTTACAGAAGAAACTGTGGTACAAAGAGAGGAAGGCTCTGGTGGCTCCTGAACAGCAGCAGTCCAGCTGCCCTGCTCAGGCTGGGCACGAC RUNX1 pro-c36160247 NO:233 GAATGCTCCCAGGAGAGGGGAGGCGGCCCACCCGACTGTGTACCGTGGACTGTGGACAGTGCAGTGCCTGCTCTCCTGTGCTATCTAGAAACACCTTGGAGAGAGGGTTCTGGGATATTCTCTCATGTCATCTGGCTGAAGACACCAGCT RUNX1 pro-c36160397 NO:234 TGACAGTTCCCCTTTAAGAAGCATTCCATACGTTTGTACCAGGGAGAAAGAAGCCCACGCACGAATTTTCAGGATGTTTTGTGACCAAACCCAATAACTGCCACACAAATAGACCCTAGGTGGGGGCTGGCTTAAATAGGACCACATTTC RUNX1 pro-c36160547 NO:235 CCGTTGCTTTTTCAGTAGATGTGTTATACCGTCATTTCAATTTGAAAGTTTATTTTTTTCCATTGATTTTTATACATCCAGAATAACACAAATAACCAACAGTTCTTTTTCTTTTTTTGCACATTCATTTCCCCTAGAACAAAAAAAGTG RUNX1 pro-c36160697 NO:236 AACTTAAAAAAAATTGGAACCATGCTATTAGCTGTTTACAAAAGTGAATAAGAAGTAGCTCATTTTAAAGTCTAAAATAAACAAAGTAGAAAGAAGCATTTTTTTCCCTACAGTATTTAGCAAACCTAAGAAAAAGTCCTTAGAAACACA BCR pro-c23522695 NO:237 GCAGAGTGCGGGCCGGGCGGGAGTGCGGCGAGAGCCGGCTGGCTGAGCTTAGCGTCCGAGGAGGCGGCGGCGGCGGCGGCGGCACGGCGGCGGCGGGGCTGTGGGGCGGTGCGGAAGCGAGAGGCGAGGAGCGCGCGGGCCGTGGCCAGA BCR pro-c23522845 NO:238 GTCTGGCGGCGGCCTGGCGGAGCGGAGAGCAGCGCCCGCGCCTCGCCGTGCGGAGGAGCCCCGCACACAATAGCGGCGCGCGCAGCCCGCGCCCTTCCCCCCGGCGCGCCCCGCCCCGCGCGCCGAGCGCCCCGCTCCGCCTCACCTGCC BCR pro-c23522995 NO:239 ACCAGGGAGTGGGCGGGCATTGTTCGCCGCCGCCGCCGCCGCGCGGGCCATGGGGGCCGCCCGGCGCCCGGGGCCGGGCTGGCGAGGCGCCGCGCCGCCGCTGAGACGGGCCCCGCGCGCAGCCCGGCGGCGCAGGTAAGGCCGGCCGCG BCR pro-c23523145 NO:240 CCATGGTGGACCCGGTGGGCTTCGCGGAGGCGTGGAAGGCGCAGTTCCCGGACTCAGAGCCCCCGCGCATGGAGCTGCGCTCAGTGGGCGACATCGAGCAGGAGCTGGAGCGCTGCAAGGCCTCCATTCGGCGCCTGGAGCAGGAGGTGA BCR pro-c23523295 NO:241 ACCAGGAGCGCTTCCGCATGATCTACCTGCAGACGTTGCTGGCCAAGGAAAAGAAGAGCTATGACCGGCAGCGATGGGGCTTCCGGCGCGCGGCGCAGGCCCCCGACGGCGCCTCCGAGCCCCGAGCGTCCGCGTCGCGCCCGCAGCCAG MRTFA pro-c40806292 NO:242 CTGTGTTTTTGTGTTTATTTTAAAAAGTTCACACACCTTCCCCATCTTTGTCCCAGCACTGGTTTTGGTGACTCCACCCCTACTCCCCTATGAGTCAGCCCAGAATGTGAAGCCAGTGGCCCCAGGGCAGGAGATGGCCACCCCTCAGCC MRTFA pro-c40806442 NO:243 CCACCGCAGGGTGAAGCTGGGCCCGAAGCTCCTCCTGTCCTAGGGCCACGCTGGCTGCAGTGAGGCGGCGGGGACGGAATGTGAGTGGGTGGAGAGCTCCTGGCAGGAAGGCCAGGTAGCACCTCTGCCCTCATGGCACTGATAAGGAGC MRTFA pro-c40806592 NO:244 CAGGAAGCAGGGCAGGTGGGGCATAGGCCGTGGCAGGGTACCTACATGTCAATCACACGTGATGGGTGGGCCTGGGTAGCTGCATGCCAGACTGGGCACCAGACTGTGGCACACAGCTCAAGGGTAACCTTGCATCCAGGCCCTTCTGTG MRTFA pro-c40806742 NO:245 AGAGTAGGATGGGAGGGAGTTGCACCCATCTCCTGTCCGCCCCCCCCCAAAAATATATATGTATGTCGATATATAATATAGAGGTATATACACCTGTACATAAAATTGTTGCCACCAACCACTAAATGGTTACACTACACCAAGACACTA MRTFA pro-c40806892 NO:246 AAATGGCAGGGAGCCCTGGGATCCTGGGGTTGGCAGACACAGGGAATAGGGGGTAGAGGCCCAGGCTAATTTCTCCCTTCACAGCAAAGCAGGGAGAGAAAGGAAGGAGATTCACCCCTTAACCTGTCTCAGCCCTGGGTCAGAAGAGAA

[0050] Example 2: Clinical Sample Testing One bone marrow sample was taken from each of three patients with clinically known positive fusion gene mutations in hematologic malignancies, and the fusion gene mutation status of these three samples was detected. Total RNA was first extracted from each of the three samples, and then cDNA synthesis and library construction were performed according to the method described in Example 1. The library construction was then completed (see...). Figure 1 ). Figure 1 The constructed library fragments were approximately 284 bp in size. The libraries captured using RA Panel probe hybridization were sequenced on a high-throughput sequencer with sequencing parameters PE: 2 × 150. After quality control of the sequenced data, bioinformatics analyses, including alignment with a reference genome, were performed to determine the fusion gene mutation status of each sample. The results are shown in Tables 20-22 below. The results were then validated using first-generation sequencing, achieving a 100% concordance rate.

[0051] The test results for sample 1 (N50144) are shown in Table 20 below: Table 20: Fusion type Mutations ReadCount ETV6--RUNX1 Detected 1403

[0052] The test results for sample 2 (N50145) are shown in Table 21 below: Table 21: Fusion type Mutations ReadCount KMT2A--MLLT3 Detected 542

[0053] The test results for sample 3 (N50072) are shown in Table 22 below: Table 22: Fusion type Mutations ReadCount AFF1--KMT2A Detected 3953

[0054] As can be seen from the detection results in Tables 20-22, the RA Panel hybridization capture probe for hematologic malignancies of the present invention can accurately capture fusion gene sites related to hematologic malignancies. The kit of the present invention can detect fusion gene mutations in hematologic malignancies, which can assist in the diagnosis and classification of hematologic malignancies, guide treatment selection, prognosis assessment, and monitoring. Detection results on routine samples show that the probe, method, and kit of the present invention can detect fusion gene mutations in hematologic malignancies.

[0055] The above description is merely a detailed explanation of preferred embodiments and principles of the present invention. For those skilled in the art, there may be changes in specific implementation methods based on the ideas provided by the present invention, and these changes should also be considered within the scope of protection of the present invention.

Claims

1. A probe set for detecting a blood tumor-associated fusion gene, characterized by, The probe set comprises a plurality of probes, and base sequences of the probes are selected from at least one of SEQ ID NO. 1~246.

2. The probe set for detecting a blood tumor-related fusion gene according to claim 1, wherein The probe coverage region comprises a blood tumor related fusion gene selected from at least one of the following group: RBM15::MRTFA, GATA2::MECOM, NPM1::MLF1, MYC::BCL6, FIP1L1::PDGFRA, KMT2A::AFF1, NUP98::NSD1, PDGFRB::ETV6, BCL11B::TLX3, DUSP22::IRF4, KMT2A::AFDN, NUP98::HOXA13, MNX1::ETV6, PCM1::JAK2, MYC::BCL2, RUNX1::RUNX1T1, NUP214::ABL1, KMT2A::MLLT3, BCR::ABL1, KMT2A::MLLT10, BIRC3::MALT1, KMT2A::ELL, KMT2A::MLLT1, ETV6::RUNX1, PML::RARA, CBFB::MYH11.

3. Use of the probe set for detecting blood tumor related fusion gene according to any one of claims 1~2 in the preparation of a library kit for detecting blood tumor related fusion gene by hybrid capture method.

4. A library construction kit for detecting blood tumor associated fusion genes, characterized in that, The library kit comprises the probe set according to any one of claims 1~2.

5. The library preparation kit for detecting blood tumor-associated fusion genes according to claim 4, characterized in that, The library kit further comprises reagents required for hybrid capture, and the reagents are selected from at least one of the following group: hybridization buffer, streptavidin magnetic beads and index primer.

6. A method for using a library preparation kit for detecting hematologic malignancy-related fusion genes, characterized in that, The steps include: Providing a biological sample, using the probe set according to any one of claims 1~2 for hybrid capture, and detecting the fusion gene by sequencing analysis.

7. The method of using the library kit for detecting blood tumor-associated fusion genes according to claim 6, wherein, The biological sample is an RNA sample.

8. The method of using the library kit for detecting blood tumor-associated fusion genes according to claim 7, wherein, Further comprising the step of constructing a cDNA library from the RNA sample.

9. The method of using the library kit for detecting blood tumor-associated fusion genes according to claim 8, wherein, The process of constructing a cDNA library from the RNA sample specifically includes RNA fragmentation, reverse transcription and end repair.

10. The method of using the library kit for detecting blood tumor-associated fusion genes according to claim 7, wherein, The starting amount of the RNA sample is 10~200 ng.

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