Single cell level proteomics analysis system and analysis method for mass spectrum system

By optimizing the sample pretreatment and data acquisition parameters of the mass spectrometry system, the problems of sample processing complexity and quantitative accuracy in single-cell proteomics analysis were solved, enabling high-throughput and accurate single-cell proteomics analysis, improving the depth and quantity of protein identification, and shortening the analysis time.

CN121431702APending Publication Date: 2026-01-30ZHEJIANG UNIV OF TECH +1
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Patent Information

Application Number
CN202511542473.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-27
Publication Date
2026-01-30

AI Technical Summary

Technical Problem

Single-cell proteomics analysis suffers from problems such as complex sample processing, insufficient detection throughput, and poor quantitative accuracy. In particular, conventional methods are difficult to meet the needs of high-precision biological research when dealing with trace samples.

Method used

By optimizing sample pretreatment and data acquisition parameters of the mass spectrometry system, including selecting appropriate buffer systems, enzymatic digestion time, and mass spectrometry parameters, and combining nanofluid chromatography with data-independent acquisition modes, protein extraction efficiency and signal intensity can be improved, and analysis time can be shortened.

Benefits of technology

It significantly improves the accuracy and reproducibility of quantitative proteomics analysis of trace samples, enables high-throughput single-cell proteomics analysis under extreme conditions, enhances the depth and quantity of protein identification, shortens analysis time, and increases daily sample analysis throughput.

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Abstract

The invention belongs to a proteomics analysis technology in the technical field of biology, and particularly relates to a single-cell-level proteomics analysis system and analysis method for a mass spectrum system. The analysis system comprises a sample pretreatment optimization subsystem and a chromatography-mass spectrometry acquisition optimization subsystem, the purpose of the sample pretreatment optimization subsystem is to efficiently and stably prepare a peptide fragment mixture capable of being used on a mass spectrum machine from a trace cell sample. The sample loss is reduced by improving the protein extraction and enzymolysis efficiency of trace (nanogram-level) and single-cell-level protein samples. By optimizing mass spectrum data acquisition parameters, the identification depth and quantity of proteins are improved while the analysis time is shortened (the flux is improved). By adopting the analysis system disclosed by the invention, the accuracy and reproducibility of quantitative analysis of the micro sample proteome can be remarkably improved.
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