Strong loquat syrup and preparation method thereof
By adding citric acid and sodium benzoate to loquat syrup to adjust the pH value, the problem of high sucrose content was solved, resulting in improved stability and antibacterial efficacy, as well as improved taste and safety.
Patent Information
- Application Number
- CN202511151939.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-18
- Publication Date
- 2026-02-03
AI Technical Summary
Existing loquat syrups have a high sucrose content, which affects human health and has a poor taste. Reducing the sucrose content will affect the density and pH stability of the finished product, making it difficult to improve the antibacterial efficacy.
By adding citric acid and sodium benzoate to loquat syrup, the pH value is adjusted to 4.4-5.0 to maintain stability and taste, while reducing sucrose content to ensure safety and antibacterial efficacy.
While reducing the sucrose content, the stability and antibacterial efficacy of the strong loquat syrup are maintained, the taste is improved, and the product safety and palatability are ensured.
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Figure CN121445833A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of traditional Chinese medicine, in particular to a strong loquat syrup and a preparation method thereof. BACKGROUND
[0002] The loquat syrup is a traditional Chinese medicine preparation mainly using loquat leaves as raw materials, which has the effects of moistening lung to stop cough, reducing sputum and relieving asthma, and is widely used for cough, excessive sputum and other symptoms caused by respiratory diseases. The loquat syrup has the effects of moistening lung to stop cough, reducing sputum and relieving asthma, clearing heat and resolving toxins, and relieving thirst.
[0003] In the original prescription of the strong loquat syrup, a large amount of sucrose is dissolved by heating, which is viscous and thick in taste, damages spleen yang, and helps to produce phlegm and dampness, which is not completely consistent with the basic principles of diagnosis and treatment of traditional Chinese medicine. Moreover, a large amount of sucrose intake will cause heart disease, high blood pressure, vascular sclerosis, cerebral hemorrhage, diabetes and other diseases, which is harmful to human health. When the amount of sucrose is large, the sweet, greasy and sticky feeling of the medicine will increase, which cannot achieve a good taste correcting effect, and high sugar content will help to produce phlegm and dampness, which may also stimulate the respiratory mucosa and cause cough. Reducing the amount of sucrose as an auxiliary material can highlight the efficacy of the main components of the preparation, and also reduce the viscosity of the syrup, improve the comfort of the taste, and have the aroma of traditional Chinese medicine.
[0004] In the prior art, the loquat syrup has a high sugar content, which is not suitable for human consumption and has poor taste. Directly reducing the sugar content in the loquat syrup will affect the density of the finished product, and it is difficult to control the pH value of the loquat syrup, and it is also difficult to improve the stability and bacteriostatic effect of the loquat syrup. SUMMARY
[0005] Therefore, the purpose of the present application is to provide a strong loquat syrup and a preparation method thereof to solve the above problems in the prior art.
[0006] In a first aspect, the present application provides a strong loquat syrup, which comprises, by mass fraction:
[0007] 60-90 parts of loquat leaves;
[0008] 40-72 parts of strychnos seed shell;
[0009] 12-32 parts of stemona;
[0010] 6-26 parts of radix baiqian;
[0011] 4-22 parts of mulberry bark;
[0012] 4-20 parts of platycodon;
[0013] 0.12-1.03 parts of menthol;
[0014] 41-62 parts of auxiliary materials;
[0015] sodium benzoate, 2.2-4.1 parts;
[0016] citric acid, 0.05-0.23 parts;
[0017] water, 500-1600 parts.
[0018] Compared with the prior art, the present application has the beneficial effects that: when reducing the sugar content in the strong loquat syrup, the pH value of the strong loquat syrup can be maintained at 4.4-5.0 by citric acid, the stability of the strong loquat syrup can be ensured, the taste of the strong loquat syrup is more comfortable, the relative density of the strong loquat syrup is maintained, and the strong loquat syrup is more stable due to the addition of sodium benzoate while reducing the sugar content, the safety of the strong loquat syrup is ensured due to the addition of sodium benzoate, and the bacteriostatic effect of the strong loquat syrup is improved due to the pH value of 4.4-5.0.
[0019] Further, the auxiliary material is sucrose.
[0020] In a second aspect, the present application also provides a preparation method of the strong loquat syrup, which is used for preparing the strong loquat syrup described above, and the method comprises the following steps:
[0021] boiling the loquat leaves, the perilla frutescens shells, the stemona, the radix bupleuri, the mulberry bark and the platycodon grandiflorum in water to obtain a decoction;
[0022] sequentially filtering and concentrating the decoction;
[0023] adding sodium benzoate to the decoction after the filtering and concentrating, and stirring to obtain a stirred liquid;
[0024] adding sucrose to the stirred liquid and heating to obtain a heated liquid;
[0025] sequentially standing and filtering the heated liquid, and adding citric acid, essence and menthol to obtain a mixed liquid;
[0026] sequentially stirring, uniformly mixing, standing and filtering the mixed liquid, and adding water to uniformly mix to obtain the strong loquat syrup.
[0027] Further, the boiling is twice boiling.
[0028] Further, the boiling time is 2-2.5 hours each time.
[0029] Further, the heating temperature is heating to boiling.
[0030] Further, the heating to boiling time is 20-30 minutes.
[0031] Further, the essence is prepared by dissolving in ethanol.
[0032] Further, after the step of obtaining the strong loquat essence, the method further comprises:
[0033] The sodium benzoate content in the strong loquat essence is checked based on high performance liquid chromatography.
[0034] Further, the step of checking the sodium benzoate content in the strong loquat essence based on high performance liquid chromatography comprises:
[0035] A preset amount of the strong loquat essence is taken out and added into a measuring flask;
[0036] Water is added to the measuring flask to dilute the preset amount of the strong loquat essence, and then shaking and filtering are sequentially performed to obtain a filtrate;
[0037] The filtrate is filtered through a microporous filter membrane to obtain a detection sample;
[0038] The detection sample is injected into a liquid chromatograph, and a chromatogram is recorded and the sodium benzoate content is calculated by an external standard method.
[0039] Further, the filtration pore size of the microporous filter membrane is 0.2-0.22 μm. BRIEF DESCRIPTION OF DRAWINGS
[0040] Figure 1 It is a sodium benzoate elution mode confirmation spectrum in the embodiment of the present application;
[0041] Figure 2 It is a sodium benzoate 190-400 nm ultraviolet absorption spectrum scanning graph in the embodiment of the present application;
[0042] Figure 3 It is a strong loquat essence specificity result comparison graph in the embodiment of the present application;
[0043] Figure 4 It is a test sample preparation method investigation comparison graph in the embodiment of the present application;
[0044] Figure 5 It is a characteristic spectrum detection graph in the embodiment of the present application.
[0045] The following specific embodiments will further illustrate the present application in combination with the above drawings. DETAILED DESCRIPTION
[0046] For the purposes of promoting an understanding of the principles of the application, reference will now be made to the embodiments illustrated in the drawings. There is shown in the drawings several embodiments of the application. However, it should be understood that the application can be practiced in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete. It is therefore an object of the present application to provide a strong loquat juice.
[0047] It is to be understood that where an element such as a layer, region or substrate is referred to as being "on" or "connected to" another element, it can be directly on or connected to the other element or intervening elements can be present. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.
[0048] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.
[0049] Embodiment One
[0050] The first embodiment of the present application provides a strong loquat juice, including loquat leaf 60 parts, poppy husk 40 parts, stemona 12 parts, baneberry 6 parts, white mulberry bark 4 parts, platycodon 4 parts, menthol 0.12 parts, auxiliary material 41 parts, sodium benzoate 2.2 parts, citric acid 0.05 parts, and water 500 parts.
[0051] In the present embodiment, the auxiliary material is sucrose.
[0052] In the present embodiment, the steps S1 to S6 are included in the preparation of the strong loquat juice.
[0053] S1, 60 parts of loquat leaf, 40 parts of poppy husk, 12 parts of stemona, 6 parts of baneberry, 4 parts of white mulberry bark, and 4 parts of platycodon are added to 200 parts of water for decoction to obtain a decoction;
[0054] S2, the decoction is sequentially filtered and concentrated;
[0055] S3, after filtering and concentrating, 2.2 parts of sodium benzoate is added to the decoction, and stirring is performed to obtain a stirring liquid;
[0056] S4, sucrose is added to the stirring liquid and heated to obtain a heated liquid;
[0057] S5, the heating liquid is sequentially placed and filtered, and 0.05 parts of citric acid, essence and 0.12 parts of menthol are added to obtain a mixed liquid;
[0058] S6, the mixed liquid is sequentially stirred, mixed, placed and filtered, and 300 parts of water is added and mixed to obtain a strong loquat syrup.
[0059] It should be noted that in this embodiment, decoction is twice decoction, and the time for each decoction is 2 hours, the heating temperature is heated to boiling, and the boiling time is kept for 20 minutes, and the essence is prepared by dissolving in ethanol.
[0060] In addition, after obtaining the strong loquat syrup, it is necessary to check the content of sodium benzoate in the strong loquat syrup, and in this embodiment, the content of sodium benzoate in the strong loquat syrup is checked based on high performance liquid chromatography.
[0061] When checking, it specifically includes steps S61 to S64:
[0062] S61, a predetermined amount of the strong loquat syrup is taken out and added to a volumetric flask;
[0063] S62, water is added to the volumetric flask to dilute the predetermined amount of the strong loquat syrup, and is sequentially shaken and filtered to obtain a filtered liquid;
[0064] S63, the filtered liquid is filtered through a microporous filter membrane to obtain a detection sample;
[0065] S64, the detection sample is injected into a liquid chromatograph, and a chromatogram is recorded and the content of sodium benzoate is calculated by an external standard method.
[0066] It should be noted that the sample is taken out 1ml, the volumetric flask is 20ml, and the pore size of the microporous filter membrane is 0.2μm.
[0067] Example two
[0068] The strong loquat syrup in the second embodiment of the application is different from the strong loquat syrup in the first embodiment.
[0069] It includes loquat leaf 70 parts, poppy husk 52 parts, stemona 17 parts, white 10 parts, white mulberry bark 8 parts, platycodon 7 parts, menthol 0.2 parts, auxiliary materials 46 parts, sodium benzoate 2.6 parts, citric acid 0.09 parts and water 900 parts.
[0070] Example three
[0071] The strong loquat syrup in the third embodiment of the application is different from the strong loquat syrup in the above-mentioned embodiments.
[0072] Loquat leaf 71 parts, meadowfoam 53 parts, stemona 18 parts, baiqian 11 parts, mulberry bark 9 parts, platycodon 8 parts, menthol 0.3 parts, auxiliary materials 47 parts, sodium benzoate 2.7 parts, citric acid 0.1 part, and water 1000 parts.
[0073] Example Four
[0074] The strong loquat juice in the fourth embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0075] Loquat leaf 72 parts, meadowfoam 54 parts, stemona 19 parts, baiqian 12 parts, mulberry bark 10 parts, platycodon 9 parts, menthol 0.35 parts, auxiliary materials 48 parts, sodium benzoate 2.8 parts, citric acid 0.11 part, and water 1050 parts.
[0076] Example Five
[0077] The strong loquat juice in the fifth embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0078] Loquat leaf 73 parts, meadowfoam 55 parts, stemona 20 parts, baiqian 13 parts, mulberry bark 11 parts, platycodon 10 parts, menthol 0.43 parts, auxiliary materials 49 parts, sodium benzoate 2.9 parts, citric acid 0.12 part, and water 1100 parts.
[0079] Example Six
[0080] The strong loquat juice in the sixth embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0081] Loquat leaf 73 parts, meadowfoam 55 parts, stemona 20 parts, baiqian 13 parts, mulberry bark 11 parts, platycodon 10 parts, menthol 0.56 parts, auxiliary materials 49 parts, sodium benzoate 2.9 parts, citric acid 0.12 part, and water 1200 parts.
[0082] Example Seven
[0083] The strong loquat juice in the seventh embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0084] Loquat leaf 75 parts, meadowfoam 58 parts, stemona 21 parts, baiqian 15 parts, mulberry bark 12 parts, platycodon 11 parts, menthol 0.62 parts, auxiliary materials 51 parts, sodium benzoate 3 parts, citric acid 0.13 part, and water 1300 parts.
[0085] Example Eight
[0086] The strong loquat juice in the eighth embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0087] Loquat leaf 76 parts, meadowfoam 59 parts, Stachys 22 parts, Rehmannia 16 parts, White Mulberry Bark 14 parts, Platycodon 12 parts, menthol 0.71 parts, auxiliary materials 52 parts, sodium benzoate 3.1 parts, citric acid 0.14 parts, and water 1350 parts.
[0088] Example Nine
[0089] The strong loquat juice in the ninth embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0090] Loquat leaf 78 parts, meadowfoam 60 parts, Stachys 23 parts, Rehmannia 17 parts, White Mulberry Bark 15 parts, Platycodon 13 parts, menthol 0.83 parts, auxiliary materials 53 parts, sodium benzoate 3.2 parts, citric acid 0.15 parts, and water 1400 parts.
[0091] Example Ten
[0092] The strong loquat juice in the tenth embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0093] Loquat leaf 79 parts, meadowfoam 61 parts, Stachys 24 parts, Rehmannia 18 parts, White Mulberry Bark 17 parts, Platycodon 14 parts, menthol 0.95 parts, auxiliary materials 54 parts, sodium benzoate 3.25 parts, citric acid 0.16 parts, and water 1450 parts.
[0094] Example Eleven
[0095] The strong loquat juice in the eleventh embodiment of the present application is different from the strong loquat juice in the above embodiments in that:
[0096] Loquat leaf 90 parts, meadowfoam 72 parts, Stachys 31 parts, Rehmannia 26 parts, White Mulberry Bark 22 parts, Platycodon 20 parts, menthol 1.03 parts, auxiliary materials 62 parts, sodium benzoate 4.1 parts, citric acid 0.23 parts, and water 1600 parts.
[0097] It is worth mentioning that in the first embodiment to the eleventh embodiment, the auxiliary materials in each embodiment are sucrose. Sucrose is a prescription auxiliary material, not a prescription medicine, and will not affect the efficacy.
[0098] In the second embodiment to the eleventh embodiment, the sugar content of the strong loquat juice is detected, and the detection results are shown in Table 1:
[0099] Table 1
[0100]
[0101] As shown in Table 1, in Examples 2 to 11, the relative density of the strong loquat syrup with sugar content of more than 45 parts ranges from 1.22 to 1.23, the pH value ranges from 4.58 to 4.79, and the sodium benzoate content is 2.4 to 2.6 mg / ml.
[0102] In addition, the strong loquat syrup in Example 1 is divided into ten samples for detection, and the detection results are shown in Table 2.
[0103] Table 2
[0104]
[0105] As shown in Table 2, the relative density of the strong loquat syrup with sugar content of 45 g after sugar reduction ranges from 1.18 to 1.19, the pH value ranges from 4.72 to 4.89, and the sodium benzoate content is 2.5 to 2.6 mg / ml.
[0106] In addition, the strong loquat syrup in Example 1 is divided into ten samples for detection, and the detection results are shown in Table 2.
[0107] Table 3
[0108]
[0109] As shown in Table 3, the properties, relative density, pH value, morphine content, and sodium benzoate content of the three samples are parallel to the results of the 10 batches of products with sugar content of 45%.
[0110] In addition, the strong loquat syrup in Example 1 is divided into ten samples for detection, and the detection results are shown in Table 2.
[0111] Table 4
[0112]
[0113] As shown in Table 4, the relative density, pH value, morphine content, and sodium benzoate content of the strong loquat syrup product after the change are stable after being placed for 10 days.
[0114] As shown in Tables 1 to 4, by detecting the relative density, pH value, morphine content, sodium benzoate content, and characteristic spectrum of the strong loquat syrup product, the quality of the preparation before and after the change of the amount of sucrose is compared and studied. The results show that the change of the amount of sucrose only affects the relative density and pH value, so the change of the sucrose content only affects the pH value of the strong loquat syrup, but the product quality remains consistent before and after the change. Reducing the sucrose content can improve the taste of the strong loquat syrup and improve the product strength.
[0115] The effect of the amount of citric acid on the pH of the strong loquat juice was tested in the control group. Three groups of strong loquat juice were used as the test group to test the effect of citric acid on the pH of the strong loquat juice. The test results are shown in Table 5:
[0116] Table 5
[0117]
[0118] The strong loquat juice in Example 1 was divided into six groups, and each group was tested. The test results are shown in Table 6:
[0119] Table 6
[0120]
[0121] The effect of the factors on the six groups of strong loquat juice was tested. The test results are shown in Table 7:
[0122] Table 7
[0123]
[0124]
[0125] From the above, it can be seen that the relative density, pH, and morphine content of the strong loquat juice placed for 10 days are stable. The test data results are within the range. Therefore, citric acid can make the strong loquat juice more stable. Therefore, by reducing the sucrose content and using citric acid, the stability of the strong loquat juice can be maintained while ensuring the original quality.
[0126] To ensure the safety and reliability of the prepared strong loquat juice, sodium benzoate needs to be checked. The content of sodium benzoate is determined by HPLC method. The chromatographic column is C18 (250 mm x 4.6 mm, 5 μm). The mobile phase is acetonitrile-0.02% formic acid (adjusted to pH 4.0 with ammonia water) 30:70. The column temperature is 30°C. The flow rate is 1 ml / min. The sample volume is 10 μl. The solvent is water. 1 ml of the sample was accurately measured and placed in a 20 ml volumetric flask. Water was added to dilute to the calibration mark. Shake well and filter. The filtrate was used as the test solution. A solution containing 0.1 mg of sodium benzoate per 1 ml was prepared as the reference solution. The calculation was performed by external standard method. The calculation formula is:
[0127]
[0128] Take 1 ml of the strong loquat juice in Example One, and take three parts, put in a 20 ml volumetric flask, respectively add water, methanol, ethanol solvent extraction, and dilution, shake and filter, take the filtrate, that is. Each test sample is prepared twice in parallel. The effects of different extraction solvents on the determination results are shown in Table 8:
[0129] Table 8
[0130]
[0131] From Table 8, it can be seen that using water, ethanol and methanol as solvent has no obvious effect on the determination results. Considering the economy and environmental protection of water, water is selected as the sample extraction solvent of strong loquat juice.
[0132] Take 1 ml of the strong loquat juice in Example One, and take three parts, put in a 20 ml volumetric flask, respectively shake, ultrasonic 10 min, 20 min extraction, cool, dilute with water to the mark, shake and filter, take the filtrate, that is. Each test sample is prepared twice in parallel. The effects of different extraction methods on the determination results are shown in Table 9:
[0133] Table 9
[0134]
[0135] From Table 9, it can be seen that using shaking, ultrasonic 10 min, ultrasonic 20 min has no obvious effect on the determination results. In order to save time, the method of shaking extraction is selected to prepare the test sample.
[0136] As shown in Figure 1 , during the detection of sodium benzoate in strong loquat juice, acetonitrile-0.02% formic acid (adjusted to pH 4.0 with ammonia water) 30:70 isocratic elution to 40 min, no other peaks appear after sodium benzoate peak, indicating that isocratic elution is complete, so it is determined as isocratic elution.
[0137] Take sodium benzoate reference substance, add water to prepare reference solution, and scan the ultraviolet absorption spectrum between 190 nm and 400 nm. The results are shown in Figure 2 , sodium benzoate has a significant absorption peak at 230 nm, which is consistent with the determination wavelength of sodium benzoate in Chinese Pharmacopoeia 2020 edition, so 230 nm is selected as the determination wavelength of sodium benzoate.
[0138] The specificity of strong loquat juice in Example One is shown in Table 10, and the comparison chart is shown in Figure 3 .
[0139] Table 10
[0140]
[0141]
[0142] Conclusion The blank solvent and the negative sample solution of sodium benzoate do not interfere with the determination of sodium benzoate. The blank solvent and the negative sample solution of sodium benzoate do not interfere with the determination of sodium benzoate. It can be seen from Table 10 that the blank solvent and the negative sample solution of sodium benzoate do not interfere with the determination of sodium benzoate.
[0143] The information of the linear solution of sodium benzoate is shown in Table 11:
[0144] Table 11
[0145]
[0146] Take 10 μl of the solutions L1-L5 respectively, inject into the liquid chromatograph, record the chromatogram, and perform linear regression on the peak area and the concentration to obtain the linear equation and calculate the linear correlation coefficient. The results are shown in Table 12:
[0147] Table 12
[0148]
[0149] Blank solvent: take water in an appropriate amount, filter through a 0.22 μm microporous filter before sampling.
[0150] Test sample solution: accurately measure 1 ml of the product, dilute to the mark in a 20 ml volumetric flask with water, shake well, filter, and take the filtrate. Filter through a 0.22 μm microporous filter before sampling. Prepare 6 samples in parallel.
[0151] Control solution ①: take an appropriate amount of sodium benzoate control, accurately weigh, and prepare a solution containing 0.1 mg per 1 ml with water. Control solution ②: take an appropriate amount of sodium benzoate control, accurately weigh, and prepare a solution containing 0.1 mg per 1 ml with water. Accurately measure 10 μl of the above solutions respectively, inject into the liquid chromatograph, and determine. Control solution ① is determined 5 times, and control solution ② and test sample solution are each determined 2 times. The results are shown in Tables 13 and 14:
[0152] Table 13
[0153]
[0154] Table 14
[0155]
[0156] The sodium benzoate in the strong loquat syrup in the above examples is limited within a safe range, so that the strong loquat syrup has stability and safety.
[0157] The characteristic spectrum of the strong loquat syrup is detected, and the related instrument information is shown in Table 15:
[0158] Table 15
[0159]
[0160] For characteristic chromatogram detection, 25 ml of the potent loquat syrup from Example 1 was weighed and placed in a 100 ml volumetric flask. Methanol was added to dilute to the mark, and the mixture was shaken well. After standing for 1 hour, it was filtered. 20 ml of the filtrate was collected, evaporated to dryness, and the residue was dissolved in 20 ml of water. The mixture was extracted three times with 20 ml of water-saturated n-butanol. The n-butanol extracts were combined, and the solvent was recovered to dryness. The residue was dissolved in an appropriate amount of methanol, transferred to a 2 ml volumetric flask, and methanol was added to the mark. The mixture was shaken well, filtered, and the filtrate was collected. Additionally, the reference standard for the characteristic chromatogram study of potent loquat syrup was chlorogenic acid with a purity of 96.3%.
[0161] The experiment investigated two different mobile phases: acetonitrile-sodium heptanesulfonate solution and acetonitrile-0.1% phosphoric acid aqueous solution. Figure 4 As shown, the acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase resulted in good resolution and a stable baseline for each chromatographic peak. Therefore, acetonitrile-0.1% phosphoric acid aqueous solution was selected as the mobile phase. The effects of column temperatures of 30℃, 35℃, and 40℃ on the retention times and resolution of each chromatographic peak were investigated. When the column temperature was 30℃, the retention times of each chromatographic peak were suitable and the resolution was good; therefore, the column temperature was set at 30℃. For detailed characteristic chromatograms, please refer to [link to chromatogram description]. Figure 5 .from Figure 5 As can be seen from the diagram, the characteristic chromatogram of the test sample should show 12 characteristic peaks. The peak corresponding to the chlorogenic acid reference peak is the S peak. The relative retention time of each characteristic peak and the S peak should be calculated, and the relative retention time should be within ±10% of the specified value. The specified values are: 0.45 (peak 1), 0.65 (peak 2), 0.75 (peak 3), 0.85 (peak 4), 0.93 (peak 5), 0.97 (peak 6), 1.05 (peak 8), 1.23 (peak 9), 1.25 (peak 10), 1.96 (peak 11), and 2.06 (peak 12).
[0162] In addition, an appropriate amount of chlorogenic acid reference substance was precisely weighed, water was added to prepare a solution containing 0.1 mg per 1 ml, i.e. a reference solution was obtained. 25 ml of the product was precisely measured and placed in a 100 ml volumetric flask, diluted to the calibration mark with methanol, shaken well, and left to stand for 1 hour. After filtration, 20 ml of the filtrate was taken, evaporated to dryness, the residue was dissolved in 20 ml of water, extracted with water-saturated n-butanol for 3 times, 20 ml each time, the n-butanol was combined, the solvent was recovered to dryness, the residue was dissolved in an appropriate amount of methanol, transferred to a 2 ml volumetric flask, added with methanol to the calibration mark, shaken well, filtered, and the filtrate was taken, i.e. a test sample solution was obtained. 5 μl of the reference solution and the test sample solution were respectively injected into the liquid chromatograph for determination. The reference solution was determined once, and the test sample solution was determined for 6 times continuously. The precision determination results are shown in Table 16, the repeatability determination results are shown in Table 17, the stability determination results are shown in Table 18, and the durability determination results are shown in Table 19. It should be noted that when the stability determination was performed, the test sample solution was obtained, and the sample was injected at 0 h, 4 h, 8 h, 12 h, 16 h and 20 h after the sample, respectively:
[0163] Table 16
[0164]
[0165] Table 17
[0166]
[0167] Table 18
[0168]
[0169] Table 19
[0170]
[0171] The results of the above detection were summarized, and the characteristic spectrum verification result summary table was obtained, as shown in Table 20:
[0172] Table 20
[0173]
[0174] It can be seen from the above that the prepared strong loquat juice has a low sucrose content, can ensure the stability, and the precision, repeatability and durability meet the requirements.
[0175] In summary, the strong loquat juice in the above-mentioned embodiments of the present application can keep the pH value of the strong loquat juice at 4.4-5.0 by citric acid when reducing the sugar content of the strong loquat juice, and can ensure the stability of the strong loquat juice, make the taste of the strong loquat juice more comfortable, keep the relative density of the strong loquat juice, and add sodium benzoate to make the strong loquat juice more stable while reducing the sugar content, and ensure the safety of the strong loquat juice while adding sodium benzoate, and the pH value of the strong loquat juice at 4.4-5.0 can also improve the bacteriostatic efficacy of the strong loquat juice.
[0176] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0177] The above-mentioned embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it cannot be understood as a limitation on the patent scope of the present application. It should be noted that, for ordinary skilled persons in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.
Claims
1. A powerful loquat syrup, characterized in that, Included by weight parts: Loquat leaves, 60-90 servings; Poppy shells, 40 to 72 parts; Stemona japonica, 12 to 32 doses; Baiqian, 6 to 26 portions; Mulberry bark, 4 to 22 parts; Platycodon grandiflorus, 4 to 20 parts; Menthol, 0.12 to 1.03 parts; Auxiliary ingredients, 41 to 62 parts; Sodium benzoate, 2.2 parts to 4.1 parts; Citric acid, 0.05 parts to 0.23 parts; Water, 500 to 1600 parts.
2. The powerful loquat syrup according to claim 1, characterized in that, The excipient is sucrose.
3. A method for preparing a potent loquat syrup, used to prepare the potent loquat syrup according to any one of claims 1 to 2, characterized in that, The method includes: Loquat leaves, poppy shells, stemona root, cynanchum root, mulberry bark, and platycodon root are boiled in water to obtain a decoction. The decoction was then filtered and concentrated sequentially. Sodium benzoate was added to the filtered and concentrated decoction and stirred to obtain a stirred liquid. Sucrose is added to the stirred liquid and heated to obtain a heated liquid; The heating liquid was allowed to stand and filtered in sequence, and citric acid, flavoring and menthol were added to obtain a mixture. The mixture is stirred, mixed, allowed to stand, and filtered in sequence, and water is added and mixed to obtain a strong loquat syrup.
4. The method for preparing the potent loquat syrup according to claim 3, characterized in that, The decoction is a two-stage decoction.
5. The method for preparing the potent loquat syrup according to claim 4, characterized in that, Each decoction session should last 2 to 2.5 hours.
6. The method for preparing the potent loquat syrup according to claim 3, characterized in that, The heating temperature is heated to boiling.
7. The method for preparing the potent loquat syrup according to claim 6, characterized in that, The heating time to boiling is 20-30 minutes.
8. The method for preparing the potent loquat syrup according to claim 3, characterized in that, The fragrance is made by dissolving in ethanol.
9. The method for preparing the potent loquat syrup according to claim 3, characterized in that, After the step of obtaining the potent loquat syrup, the method further includes: The sodium benzoate content in the strong loquat syrup was determined by high performance liquid chromatography.
10. The method for preparing the potent loquat syrup according to claim 9, characterized in that, The steps for determining the sodium benzoate content in the strong loquat syrup based on high performance liquid chromatography include: Take out a predetermined amount of the strong loquat syrup and add the predetermined amount of the strong loquat syrup into a volumetric flask; Add water to the volumetric flask to dilute a predetermined amount of the strong loquat syrup, and then shake and filter the solution to obtain the filtrate. The filtrate is filtered through a microporous membrane to obtain the test sample; The test sample is injected into a liquid chromatograph, and the chromatogram is recorded, and the sodium benzoate content is calculated by the external standard method.
11. The method for preparing the potent loquat syrup according to claim 10, characterized in that, The microporous filter membrane has a pore size of 0.2μm - 0.22μm.