Hydroponic cuttage method for twigs with red and light branches
By combining hydroponic substrate and growth regulators, the high cost of environmental control and pest and disease problems in the red-light ratio cutting method are solved, achieving rapid rooting and low-cost cutting propagation, which is suitable for industrial production and international development.
Patent Information
- Application Number
- CN202511983493.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-26
- Publication Date
- 2026-02-06
AI Technical Summary
The existing red-to-light ratio cutting method requires strict control of environmental conditions, is costly, and is difficult to control for pests and diseases, thus failing to meet the needs of rapid propagation and economic benefits.
Using a hydroponic substrate and growth regulators such as naphthaleneacetic acid (NAA), indolebutyric acid (IBA), or rooting powder (ABT), and combined with tap water, distilled water, or lake water as the water source, hydroponic cuttings of tender red and light-colored shoots are propagated. The pH value is controlled and the cuttings are shaded to promote rapid rooting.
It achieves rapid rooting, increases the rooting rate, reduces costs, is easy to operate, is suitable for factory production, reduces pests and diseases, and promotes the standardization and internationalization of regional planting.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of plant asexual reproduction, and relates to a cutting method, in particular to a method for rapid rooting of red-tanpi tender branch water culture cutting. BACKGROUND
[0002] Red-tanpi ( Cleyera japonica ) is a evergreen shrub or small tree of the family of pentaphylacaceae, and is a rare native tree species in China. Red-tanpi is mainly distributed in the areas south of the middle and lower reaches of the Yangtze River and Taiwan Province of China. Red-tanpi has a wide distribution range, but is sporadic and scattered, and the resource quantity is not much. Moreover, red-tanpi has high economic, medicinal and ornamental values and garden application prospects.
[0003] For a long time, red-tanpi is mainly propagated by seeds and cutting. As for seed propagation, the seedlings are uneven in height and thickness, have large differences, and grow relatively slowly, which cannot meet the demand for rapid propagation of resources. Therefore, it is very important to research and improve the cutting propagation method of red-tanpi. Cutting has the advantages of sufficient propagation materials, large propagation coefficient, large yield of seedlings, fast seedling formation, early flowering, and maintaining the excellent characteristics of the original variety, so the cutting technology of red-tanpi is researched.
[0004] The existing cutting method of red-tanpi mostly uses perlite, peat soil and other culture media as the support of cutting seedlings, but needs to strictly control the environmental conditions and cultivation management, has high cost, and is difficult to control diseases and pests. The traditional propagation method cannot meet the current social demand for the species. SUMMARY
[0005] In view of the shortcomings and deficiencies in the prior art, the purpose of the present application is to provide a high-efficiency, environmentally-friendly and low-cost method for water culture cutting of red-tanpi tender branches, so as to provide a reference for the cutting propagation and cultivation management of red-tanpi.
[0006] In order to achieve the above-mentioned purpose of the application, the present application provides the following technical scheme: A method for water culture cutting of red-tanpi tender branches, comprising the following steps: (1) Collection and preparation of cuttings: in spring or autumn, select the current year's tender branches of red-tanpi mother trees without diseases and with healthy growth; trim the branches to 12-15 cm as cuttings, the cuttings have 1-2 buds and 1-2 leaves; (2) Preparation of rooting agent: use any one or more of 100-500 mg / L naphthaleneacetic acid NAA, 100-500 mg / L indolebutyric acid IBA and 100-500 mg / L rooting powder ABT; (3) Water culture: use tap water, distilled water or lake water as the water source for water culture cutting; (4) cutting: the cutting is washed with clean water, the base is disinfected with 0.5% potassium permanganate by mass percentage, then is quickly soaked in the rooting agent for 1h, is inserted into the solution of the water culture, and is sprayed with the rooting agent and Bordeaux solution after cutting, and is covered with plastic cloth for moisture retention; (5) post-cutting management: the pH value in the water culture solution is kept neutral or slightly alkaline, the water is changed once a day during cutting, and shading treatment is carried out in the first two weeks after cutting.
[0007] Further, the rooting agent is 100-500 mg / L indole butyric acid IBA.
[0008] Further, the solution of the water culture is distilled water.
[0009] Further, the upper end of the cutting is cut horizontally at a position 0.5 cm away from the axillary bud, and the lower end of the cutting is obliquely cut at a position away from the axillary bud at an angle of 45°.
[0010] Further, the tap water is aired for at least 1 day to remove chlorine.
[0011] Further, the device of the water culture is a water culture box, the water culture box is a black plastic box, the volume of the water source loaded is 1 / 2 of the water culture box, a foam plate is placed in the box, the foam plate is about 1 cm away from the upper end of the water culture solution, 50-80 circular holes of the same size are dug on each foam plate.
[0012] Further, the cutting and the water culture box are disinfected with 0.1% potassium permanganate by mass percentage.
[0013] Further, the cutting is placed in the solution of the water culture, and the cutting is 3-5 cm deep.
[0014] Advantages of the present application are as follows: The water culture cutting method of the tender branch of the red and thin ratio provided by the present application is cultured by using a water culture medium and the rooting is stimulated by increasing hormones, so that the rooting time is short, the rooting speed is fast, and the rooting rate is high.
[0015] Compared with the existing traditional cutting method of the red and thin ratio, the water culture cutting method of the present application is more effective, environmentally friendly and low in cost.
[0016] The water culture cutting method of the tender branch of the red and thin ratio provided by the present application is convenient to operate, easy to manage after cutting and transplanting and planting, low in production and management cost, beneficial to factory production in large quantities to bring economic benefits, and can promote the standardization of regional planting, enrich the whole industry chain, and further go to the internationalization. BRIEF DESCRIPTION OF DRAWINGS
[0017] Figure 1These are images showing the results of traditional soil-cultivated cuttings with varying shades of red compared to tender shoots: A. Initial morphology of the cutting; B. Callus formation at the basal cut; C. Abundant rooting in the bark; Figure 2 The following are the results of hydroponic cultivation of tender branches using the red and light color ratio method of this invention: A. Bark cracking; B. Adventitious root emergence; C. Numerous roots growing in the bark. Detailed Implementation
[0018] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0019] This invention provides a method for hydroponic propagation of tender branches with varying shades of red, including the following steps: (1) Hydroponic cultivation: Tap water, distilled water or lake water can be used as the water source for hydroponic cuttings. However, the residual chlorine in tap water can cause root rot. Therefore, before hydroponic cuttings, tap water needs to be dechlorinated and left to stand for 1 day before use.
[0020] The hydroponic device is a hydroponic box (a black plastic box). The hydroponic box is filled with tap water, distilled water or lake water (the volume of the liquid is about 1 / 2 of the hydroponic box). A foam board (planting board) is placed in the box. The foam board is about 1cm away from the top of the hydroponic solution. Each foam board has 50-80 round holes of the same size (4cm in diameter).
[0021] Place the cuttings in a hydroponic solution with appropriate parameters, to a depth of about 4 cm.
[0022] (2) Collection and preparation of cuttings: In spring or autumn, select healthy, disease-free, and vigorous young branches of the current year from the mother tree as cuttings. Cut the cuttings to be 12-15cm long and of the same thickness, with 1-2 buds and 1-2 leaves. Make a horizontal cut 0.5cm away from the axillary bud at the top and a 45° oblique cut at the bottom away from the axillary bud at the bottom to obtain the cuttings.
[0023] (3) Preparation of rooting agent: The rooting agent (growth regulator) of the present invention includes 100~500 mg / L of NAA (naphthaleneacetic acid) and 100~500 mg / L of One or more of IBA (indolebutyric acid) or rooting powder ABT No. 1 (purchased from Beijing Aibit Biotechnology Co., Ltd., with a total effective ingredient content of 50%, including 20% NAA and 30% IAA) at a concentration of 100~500 mg / L, should be prepared and used immediately with distilled water.
[0024] (4) Cutting technique: Wash the cuttings from step (2) with clean water, then disinfect the base with 0.5% potassium permanganate for 5 minutes; then quickly soak them in the rooting agent prepared in step (3) for 1 hour, and then insert them into the hydroponic cutting substrate (hydroponic solution from step (1)).
[0025] After inserting the cuttings, spray the above-mentioned rooting agent once (the above-mentioned rooting agent is a special care measure for foliar fertilization or assisting rooting, which aims to provide necessary nutrients and / or rooting stimulants to the cuttings when they have not yet grown roots or when the root system is weak, so as to improve the survival rate and accelerate growth), spray Bordeaux mixture, and cover with plastic sheeting to keep it moist.
[0026] (5) Post-insertion management: Keep the pH value of the hydroponic solution neutral or slightly alkaline (pH=7.0~7.5), change the water once a day during the cutting period, and provide shade for the first two weeks after cutting. Example 1
[0027] A method for hydroponic propagation of light red and tender shoots using young branches, the steps are as follows: Hydroponic propagation of young shoots of *Rhododendron simsii* can be carried out in spring or autumn. In this example, it is done in mid-September. Select healthy, disease-free young shoots from the current year of a healthy *Rhododendron simsii* mother tree as cuttings. Cut the shoots to be 12-15 cm long and of uniform thickness, with 1-2 buds and 1-2 leaves. Make a horizontal cut 0.5 cm from the axillary bud at the top, and a 45° oblique cut at the bottom away from the axillary bud at the bottom. Immerse the lower 1 / 3 of the cutting in a 100 mg / L NAA solution for 1 hour. Then, insert the cuttings into a hydroponic substrate of tap water. After insertion, spray with nutrient solution once, spray with Bordeaux mixture, and cover with plastic wrap to retain moisture. Maintain the pH of the hydroponic solution at neutral or slightly alkaline. Change the water once a day during the propagation period, and provide shade for the first two weeks after insertion.
[0028] Thirty days after cutting, record the number of new roots. When the number of roots is greater than 5, transplant the rooted stem segments into the soil to begin hardening off. For the first 3 days of hardening off, avoid direct sunlight and water once in the morning and once in the evening. Transplanting can be done one week after hardening off. Example 2
[0029] The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was an IBA solution with a concentration of 300 mg / L. Example 3
[0030] The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was a rooting powder (ABT1) solution with a concentration of 500 mg / L. Comparative Example 1
[0031] A method for soil-based propagation of reddish-brown shoots compared to young branches, the steps of which are as follows: Red peony rootstock cuttings can be propagated in spring or autumn. In this example, in mid-September, select healthy, disease-free, current-year shoots from vigorous red peony rootstock mother trees as cuttings. Cut the shoots to 12-15cm in length and uniform thickness, with 1-2 buds and 1-2 leaves. Make a horizontal cut 0.5cm from the axillary bud at the top and a 45° oblique cut away from the axillary bud at the bottom. Immerse the lower 1 / 3 of the cutting in a 100mg / L NAA solution for 1 hour. Then, insert the cuttings into sterilized peat moss propagation substrate. After insertion, spray with nutrient solution once, apply Bordeaux mixture, and cover with plastic wrap to maintain humidity. Keep the pH of the hydroponic solution neutral or slightly alkaline, change the water daily during the propagation period, and provide shade for the first two weeks after insertion.
[0032] Thirty days after cutting, record the number of new roots. When the number of roots is greater than 5, transplant the rooted stem segments into the soil to begin hardening off. For the first 3 days of hardening off, avoid direct sunlight and water once in the morning and once in the evening. Transplanting can be done one week after hardening off. Comparative Example 2
[0033] The method in Comparative Example 1 was used for soil-cultured cuttings of red and light-colored tender branches, except that the plant growth hormone used was an IBA solution with a concentration of 300 mg / L. Comparative Example 3
[0034] The method in Comparative Example 1 was used for soil propagation of red and light-colored tender branches, except that the plant growth hormone used was a rooting powder (ABT1) solution with a concentration of 500 mg / L. Example 4
[0035] The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was a 300 mg / L NAA solution, and distilled water was used as the hydroponic propagation substrate.
[0036] Example 5 The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was an IBA solution with a concentration of 500 mg / L, and distilled water was used as the hydroponic propagation substrate.
[0037] Example 6 The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was a rooting powder (ABT1) solution with a concentration of 100 mg / L, and distilled water was used as the hydroponic propagation substrate.
[0038] Example 7 The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was a 500 mg / L NAA solution, and lake water was used as the hydroponic propagation substrate.
[0039] Example 8 The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was an IBA solution with a concentration of 100 mg / L, and lake water was used as the hydroponic propagation substrate.
[0040] Example 9 The method described in Example 1 was used for hydroponic propagation of red and light-colored tender branches, except that the plant growth hormone used was a rooting powder (ABT1) solution with a concentration of 300 mg / L, and lake water was used as the hydroponic propagation substrate.
[0041] To screen plant growth regulators for different types, concentrations, and hydroponic substrates, a three-factor, three-level experiment was conducted, as shown in Table 1. The applicant conducted a comparative experiment.
[0042] Starting from day 25 of cutting and ending at day 50 of cutting, the rooting rate, average number of roots, average root length, and time of rooting onset of the hydroponic cutting method for red and light-colored tender shoots in Examples 1-9 were statistically analyzed. Each treatment was replicated three times, and the results are shown in Table 2.
[0043] Table 1-L9(34) Factor Levels in Orthogonal Experimental Design of Red-Light Ratio and Softwood Cuttings
[0044] Table 2-L9(34) Red-to-light ratio Red-to-light ratio Relevant parameters for softwood cuttings
[0045] As shown in Table 2, the survival rate of cuttings varies significantly depending on the substrate hormone formulation.
[0046] contrast Figure 1 and Figure 2 It is evident that hydroponics results in a higher survival rate, faster growth time, healthier root systems, and freedom from pests and diseases compared to traditional soil cultivation. Specifically, from... Figure 1 It is known that traditional soil cultivation methods have a low rooting rate, a long rooting time, and are susceptible to pests and diseases. Figure 2 It can be seen that the hydroponic method of the present invention has a higher rooting rate, shorter rooting time, and is more efficient, environmentally friendly, and low-cost than soil culture.
[0047] Furthermore, the matrix formula of the present invention (Example 5: distilled water + IBA + 500 mg / L) resulted in the highest survival rate of red and light-colored tender branches, reaching 85.33%.
[0048] In summary, the above are merely preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A method for hydroponic propagation of tender red and light-colored branches, characterized in that, The method includes the following steps: (1) Collection and preparation of cuttings: In spring or autumn, select healthy, disease-free, and vigorous young branches of the current year from the mother tree; cut the above branches to 12-15cm as cuttings, with 1-2 buds and 1-2 leaves. (2) Preparation of rooting agent: Use any one or more of naphthaleneacetic acid (NAA), indolebutyric acid (IBA), or rooting powder (ABT); (3) Hydroponic cultivation: Tap water, distilled water or lake water are used as the water source for hydroponic cuttings; (4) Cuttings: After washing the cuttings with clean water, disinfect the base with 0.5% potassium permanganate by mass, then quickly soak them in the rooting agent for 1 hour, and insert them into the hydroponic culture solution; after insertion, spray the rooting agent, spray Bordeaux mixture, and cover with plastic sheeting to keep moist. (5) Post-insertion management: Keep the pH value of the hydroponic solution neutral or slightly alkaline, change the water once a day during the cutting period, and shading treatment for the first two weeks after cutting.
2. The method according to claim 1, characterized in that, The concentrations of naphthaleneacetic acid (NAA), indolebutyric acid (IBA), and rooting powder (ABT) are all 100-500 mg / L.
3. The method according to claim 1 or 2, characterized in that, The rooting agent is 100-500 mg / L indolebutyric acid (IBA).
4. The method according to claim 1 or 2, characterized in that, The hydroponic culture solution is distilled water.
5. The method according to claim 1 or 2, characterized in that, The upper end of the cutting is cut horizontally 0.5cm away from the axillary bud, and the lower end of the cutting is cut at a 45° angle away from the axillary bud.
6. The method according to claim 1 or 2, characterized in that, Let the tap water sit for at least one day to remove chlorine.
7. The method according to claim 1 or 2, characterized in that, The hydroponic cultivation device is a hydroponic box, which is a black plastic box. The volume of water in the hydroponic box is 1 / 2 of the volume. A foam board is placed inside the box, about 1 cm away from the top of the hydroponic solution. Each foam board has 50-80 round holes of the same size cut out.
8. The method according to claim 7, characterized in that, After the cuttings and hydroponic boxes are made, they are disinfected with 0.1% potassium permanganate by weight.
9. The method according to claim 1 or 2, characterized in that, The cuttings are placed in the hydroponic culture solution at a depth of 3-5 cm.