Water-saving organic fertilizer based on synergistic fermentation of discarded pregnant mare urine and multi-source feces and preparation method and application thereof

Abstract

This invention relates to the field of agricultural waste resource utilization technology, providing a water-saving organic fertilizer based on the co-fermentation of waste pregnant mare urine and multi-source manure, its preparation method, and its application. This invention uses waste pregnant mare urine (after extracting and binding estrogen) as the core nitrogen source and the sole source of process water, mixing it with multi-source manure and high-carbon auxiliary materials in a specific ratio, and adding a certain amount of zeolite powder. Utilizing the adsorption characteristics of zeolite powder and the synergistic effect of adsorption-enrichment-degradation formed during the high-temperature composting process, hormone residues are completely eliminated and ammonium nitrogen is fixed. Aerobic fermentation is carried out through a two-stage temperature-controlled turning process to produce safe, efficient, and high-quality organic fertilizer. This invention achieves the harmlessness and resource utilization of high-risk pharmaceutical waste, with hormone residues in the finished product below 1 μg / kg; a water-saving rate of 100%; zeolite powder reduces nitrogen loss rate from >40% to <15%; zeolite also effectively adsorbs odorous gases, reducing emissions, providing pharmaceutical companies with an economical and environmentally friendly new approach to waste treatment.

Description

Technical Field

[0001] This invention relates to the field of agricultural waste resource utilization technology, specifically to a water-saving organic fertilizer based on the synergistic fermentation of waste pregnant mare urine and multi-source manure, its preparation method and application. Background Technology

[0002] In recent years, conjugated equine estrogens (CEEs) have become an important pharmaceutical raw material, primarily extracted from pregnant mare urine. The production of 1 kg of CEEs generates approximately 150-200 tons of waste pregnant mare urine. This waste liquid not only possesses the high nitrogen and potassium characteristics of ordinary mare urine, but also suffers from high salinity (EC values ​​can reach 10-15 mS / cm or higher) and potential residual trace amounts of hormonal active substances, making its environmental treatment difficult and costly, thus becoming an environmental bottleneck for related pharmaceutical companies. Currently, this type of waste liquid is often treated using expensive wastewater treatment processes, and its rich nitrogen and potassium nutrients are not utilized but instead become an environmental burden.

[0003] In traditional composting operations, to maintain the humidity (50%-60%) required for microbial activity, frequent spraying of fresh water is necessary during the composting process. This is particularly problematic in dry, hot, or windy areas, where water consumption is significant, increasing operating costs. Furthermore, under conditions of high temperature, high pH, ​​and frequent turning, traditional composting leads to a significant increase in ammonium nitrogen (NH4+) in the compost pile. + It is easily converted into ammonia (NH3) and volatilized, with nitrogen loss rate as high as 30%-50% of total nitrogen, resulting in reduced fertilizer efficiency and damaged economic value of the final compost product.

[0004] Therefore, developing a technology that can simultaneously achieve the harmlessness and resource utilization of waste pregnant mares' urine, and completely solve the problems of water conservation and fertilizer retention in traditional composting, has extremely high industrial application value and environmental significance. Summary of the Invention

[0005] The purpose of this invention is to provide a water-saving organic fertilizer based on the co-fermentation of waste pregnant mare urine and multi-source manure, as well as its preparation method and application.

[0006] In order to achieve the purpose of this invention, in a first aspect, this invention provides the application of waste pregnant mare urine in fertilizer preparation.

[0007] The waste pregnant mare urine of this invention is waste pregnant mare urine after extraction and binding with estrogen.

[0008] In this invention, the EC conductivity of the discarded pregnant mare urine is in the range of 8-15 mS / cm (preferably in the range of 12-15 mS / cm), the pH value is 8.5-9.5, and the estrone equivalent residue is 50-60 μg / L.

[0009] Secondly, the present invention provides a method for preparing water-saving organic fertilizer based on the co-fermentation of waste pregnant mare urine and multi-source feces, wherein the waste pregnant mare urine is waste pregnant mare urine after extraction and binding with estrogen.

[0010] Includes the following steps:

[0011] S1. Material pretreatment: Collect the waste pregnant mare urine, sheep manure, horse manure, cow manure and high-carbon auxiliary materials respectively, mix the sheep manure, horse manure, cow manure and high-carbon auxiliary materials and crush them to a particle size of less than 5cm to obtain mixed solid materials;

[0012] S2. Mixing and Batching: Mix the mixed solid material obtained in step S1, waste pregnant mare urine and zeolite powder in proportion, stir and mix well, and adjust the initial moisture content of the pile to 50%-60%; the waste pregnant mare urine is the only source of moisture for adjusting the moisture content of the pile, and no additional fresh process water is required.

[0013] S3. Inoculation agent: Spray the compound microbial fermentation agent onto the mixture obtained in step S2;

[0014] S4. Composting: The inoculated mixture is piled into a trapezoidal pile with a width of 1.5-2.5m and a height of 1.0-1.5m, and covered with a breathable material for fermentation;

[0015] S5. Fermentation Management: Monitor the temperature of the fermentation pile and control the fermentation process, which is divided into a high-temperature stage and a cooling stage;

[0016] The high-temperature phase involves maintaining the reactor body temperature at 55-65℃ for 10-15 days; the cooling phase involves controlling the reactor body temperature at 29-50℃ for 15-25 days.

[0017] When the temperature rises to 60-65℃, the pile is turned over for the first time. Subsequently, the pile is turned over every time the temperature exceeds 65℃. The entire fermentation process involves turning the pile 3-4 times.

[0018] S6. Post-fermentation and evaluation: When the temperature of the compost pile drops to the ambient temperature and remains stable, and the material is dark brown and odorless, fermentation is complete, and the organic fertilizer is obtained.

[0019] Further, the volume ratio of the mixed solid material, horse urine, and high-carbon auxiliary material is (50%-70%):(20%-40%):(5%-15%), and the total volume percentage is 100%. Preferably, the volume ratio of the mixed solid material, horse urine, and high-carbon auxiliary material is (50%-60%):(30%-40%):(10%-20%). More preferably, the volume ratio of the mixed solid material, horse urine, and high-carbon auxiliary material is 60%:30%:10%.

[0020] Furthermore, in the mixed solid material, the volume ratio of sheep manure, horse manure, and cow manure is (3-5):(2-4):(3-5).

[0021] Further, in step S2, the amount of zeolite powder added is 3%-8% of the total weight of the total material, and the particle size is 80-200 mesh (preferably 5% with a particle size of 100 mesh).

[0022] The total material refers to a mixture of mixed solid materials, horse urine, and high-carbon auxiliary materials.

[0023] Further, in step S3, the compound microbial fermentation agent contains Bacillus subtilis (… Bacillus subtle ), Bacillus retroflexus ( Brevibacillus laterosporus ) and brewer's yeast ( Saccharomyces yeast );

[0024] The agent is formulated with Bacillus subtilis, Bacillus laterosporus, and Saccharomyces cerevisiae in a viable cell ratio of (1-2):(1-1.5):(1-2); the total effective viable cell count of the agent is 5.0 × 10⁻⁶. 9 ~6.0×10 9 The inoculum concentration is CFU / g, and the inoculum amount is 0.1%-0.2% (preferably 0.1%) of the total weight of the mixture.

[0025] Preferably, the compound microbial fermentation agent is a mixture of Bacillus subtilis, Bacillus laterosporus, and Saccharomyces cerevisiae in a live cell ratio of 2:1:1. Further, the high-carbon auxiliary material includes crop straw, such as wheat straw.

[0026] Thirdly, the present invention provides an organic fertilizer prepared by the method, wherein the organic fertilizer contains zeolite powder and, based on estrone, has a natural conjugated estrogen activity equivalent of less than 1 μg / kg.

[0027] Fourthly, the present invention provides any of the following applications of the said organic fertilizer:

[0028] (1) Used for plant cultivation, such as planting vegetables, fruit trees, tea trees or flowers;

[0029] (2) Used to improve soil.

[0030] By employing the above technical solution, the present invention has at least the following advantages and beneficial effects:

[0031] This invention uses waste pregnant mare urine (after extracting and binding estrogen) as the core nitrogen source and the sole source of process water. It is mixed with high-carbon auxiliary materials such as sheep manure, horse manure, cow manure, and straw in a specific ratio, and innovatively, zeolite powder is added at 3-8% of the total weight of the mixture. Utilizing the adsorption properties of zeolite powder and the synergistic effect of "adsorption-enrichment-degradation" formed during the high-temperature composting process, hormone residues are completely eliminated and ammonium nitrogen is fixed. Aerobic fermentation is carried out through a two-stage temperature-controlled turning process, ultimately producing safe, efficient, and high-quality organic fertilizer.

[0032] (i) 100% water saving and waste resource utilization: all process water is replaced by waste pregnant mare urine, achieving a 100% water saving rate and realizing the internal circulation of water resources and the value-added utilization of high-risk waste liquid.

[0033] (ii) High-efficiency nitrogen fixation and fertilizer retention: The addition of zeolite powder reduces the nitrogen loss rate from >40% in traditional methods to below 15%, greatly improving the fertilizer efficiency and economic value of the product.

[0034] (III) Thorough harmless treatment: The synergistic effect of "zeolite adsorption + high temperature degradation" can effectively ensure that the estrogen activity equivalent in the final product is less than 1 μg / kg (calculated as estrone), making the environment safe and reliable.

[0035] (iv) Environmental friendliness and quality improvement: Zeolite effectively adsorbs malodorous gases and significantly reduces emissions of ammonia and other gases; the complementary use of multiple materials optimizes the carbon-nitrogen ratio, shortens the fermentation cycle, and increases the humic content. Detailed Implementation

[0036] The present invention aims to overcome the shortcomings of the prior art and provide an organic fertilizer preparation method that can solve the following three key problems in one integrated way: (1) realize the harmless and resource-based treatment of waste pregnant mare urine: especially effectively remove its high salt content and trace residual hormones; (2) significantly reduce or even eliminate process water in the composting process: reduce production costs and resource consumption; (3) significantly reduce nitrogen loss in the composting process: improve the fertilizer efficiency and value of the final organic fertilizer product.

[0037] Therefore, developing a technology that can simultaneously achieve the harmlessness and resource utilization of waste pregnant mares' urine, and completely solve the problems of water conservation and fertilizer retention in traditional composting, has extremely high industrial application value and environmental significance.

[0038] In order to simultaneously achieve the harmless treatment of waste pregnant mare urine (especially the removal of its high salt content and trace residual hormones), 100% replacement of process water in the composting process, and efficient retention of nitrogen, this invention provides a water-saving organic fertilizer preparation method and product based on the co-fermentation of waste pregnant mare urine generated in the pharmaceutical industry and multi-source manure.

[0039] The present invention adopts the following technical solution:

[0040] This invention provides a method for preparing high-value water-saving organic fertilizer by co-fermenting waste pregnant mare urine generated in the pharmaceutical industry with various livestock and poultry manures. The core of the method is to use waste pregnant mare urine after extracting and binding estrogen as the core nitrogen source and the only source of process water, mix it with high-carbon auxiliary materials such as sheep manure, horse manure, cow manure and straw in a specific ratio, and innovatively add zeolite powder accounting for 3-8% of the total weight of the mixture.

[0041] This invention recognizes that the challenges in treating waste pregnant mare urine can be addressed by combining it with composting processes. Its high salt content can be diluted by a large amount of solid material and transformed through microbial degradation; its high moisture content can completely replace process water. Furthermore, the addition of zeolite powder and a high-temperature fermentation process at ≥55℃ for more than 10 days can create a synergistic effect.

[0042] Regarding the harmlessness of hormones: The porous structure of zeolite powder can effectively adsorb trace hormone molecules remaining in pregnant mare urine, enriching them in the pores. This not only reduces the free migration of hormones in the aggregate but also allows them to be more thoroughly degraded by Bacillus laterosporus under subsequent sustained high temperatures, forming a synergistic effect of "adsorption-enrichment-degradation" and ensuring the absolute environmental safety of the product.

[0043] Regarding nitrogen fixation and fertilizer retention: Zeolite powder has a positive effect on ammonium ions (NH4+). + It has a highly selective adsorption capacity, which can firmly fix the ammonium nitrogen produced during composting in its pores, greatly reducing the loss of nitrogen in the form of ammonia (NH3).

[0044] Regarding salinity and control: High-salt pregnant mare urine is diluted and adsorbed by a large amount of solid materials and zeolite powder, and through the transformation of microorganisms, the EC value of the final product is eventually reduced to a safe range.

[0045] This invention achieves the harmlessness and resource utilization of high-risk pharmaceutical waste, with hormone residue in the finished product being less than 1 μg / kg; water saving rate of 100%; zeolite powder reduces nitrogen loss rate from >40% to <15%; zeolite also effectively adsorbs malodorous gases, reducing emissions, and provides pharmaceutical companies with a new economical and environmentally friendly waste treatment approach.

[0046] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0047] Bacillus subtilis used in the following examples Bacillus subtilis Lateral spores of Bacillus brevis Brevibacillus laterosporus and brewing yeast Saccharomyces cerevisiae Purchased from Hubei Qiming Biotechnology Co., Ltd.

[0048] The waste pregnant mare urine used in the following examples is waste pregnant mare urine after extracting and conjugating estrogen. The preparation process of conjugated estrogen can be found in CN1381240A.

[0049] In the following embodiments, the moisture content of the mixed solid materials is approximately 40%, and the moisture content of the straw is approximately 10%.

[0050] Example 1:

[0051] Raw materials: Waste pregnant mare urine after estrogen conjugation (tested to have pH=8.9, EC=12.4mS / cm, and estrone equivalent residue of approximately 35μg / L), fresh sheep manure, horse manure, and cow manure (volume ratio 4:3:3), wheat straw fragments, 100-mesh clinoptilolite powder, and Bacillus subtilis. Bacillus subtilis Lateral spores of Bacillus brevis Brevibacillus laterosporus and brewing yeast Saccharomyces cerevisiae When formulated with a live bacteria ratio of 2:1:1, the total effective live bacteria count is approximately 5.5 × 10⁻⁶. 9 CFU / g).

[0052] Process:

[0053] 1. Mix solid manure with straw and crush it to a particle size of <5cm.

[0054] 2. Prepare materials according to the volume ratio of mixed solid material: horse urine: straw = 60% : 30% : 10%, and add 5% of zeolite powder by weight of the total materials, and dry mix evenly.

[0055] 3. Slowly pour in the waste pregnant mare urine while stirring, until the mixture reaches a moisture content of 55%.

[0056] 4. Inoculate with 0.1% compound microbial agent (0.1% of the total weight of the mixture), build a pile (2m wide × 1.2m high), and cover with breathable felt.

[0057] Fermentation management: On the third day, the temperature rises to 63℃, and the pile is turned for the first time; on the third day after the first turning, the temperature rises to 70℃. The high-temperature stage (≥55℃) is maintained for 12 days, with a total of 3 turnings. After that, the cooling stage begins, and on the 35th day, the pile temperature drops to 29℃, the material turns dark brown, and has a slight earthy smell, indicating that fermentation is complete.

[0058] Effect detection:

[0059] 1. Safety: The finished product was tested by HPLC-MS / MS, and the residues of various natural conjugated estrogens were all below the method detection limit (<1μg / kg).

[0060] 2. Salt content: The EC value of the finished product is reduced to 3.8 mS / cm.

[0061] 3. Fertilizer efficiency: Total nutrient (N+P2O5+K2O) content ≥5.8%, organic matter content ≥46%, seed germination index (GI) is 92%.

[0062] Comparative Example 1: (No zeolite powder, using ordinary horse urine)

[0063] All other conditions were exactly the same as in Example 1, except that zeolite powder was not added and ordinary horse urine was used (no risk of hormone residue, EC=5.2mS / cm).

[0064] Results: A distinct ammonia odor was detected during fermentation. The final product had a total nitrogen content of 2.1%, a 25% reduction compared to Example 1 (2.8%), with an estimated nitrogen loss rate of 38%. This demonstrates the crucial role of zeolite powder in nitrogen fixation.

[0065] Comparative Example 2: (Traditional method, with the addition of water)

[0066] Instead of using horse urine, 30% of the pregnant mare urine in Example 1 was replaced with an equal amount of clean water to adjust the humidity.

[0067] Results: A large amount of water was required. The total nitrogen content of the fermented product was only 1.9%, resulting in a severe nitrogen loss (approximately 44%), and the nutrients in the horse urine were completely wasted.

[0068] Comparative Example 3: (Zeolite-free treatment of hazardous waste – verifying the key to hormone harmlessness)

[0069] All other conditions were exactly the same as in Example 1, except that waste pregnant mare urine was used, but no zeolite powder was added.

[0070] Results: Trace amounts of estrone (approximately 5.8 μg / kg) were still detected in the fermented product by HPLC-MS / MS. This comparison demonstrates that, when treating such high-risk wastes, relying solely on high-temperature fermentation is insufficient to fully guarantee the purification effect of hormones; the synergistic effect of "zeolite adsorption-high-temperature degradation" is an indispensable key.

[0071] Example 2:

[0072] Raw materials: Waste pregnant mare urine after estrogen extraction (tested to be pH=8.9, EC=12.4mS / cm, estrone equivalent residue approximately 30μg / L), fresh sheep manure, horse manure, and cow manure (volume ratio 3:4:3), wheat straw fragments, 100-mesh zeolite powder, and compound fermentation agent (Bacillus subtilis). Bacillus subtilis Lateral spores of Bacillus brevis Brevibacillus laterosporus and brewing yeast Saccharomyces cerevisiae When formulated with a live bacteria ratio of 2:1:2, the total effective live bacteria count is approximately 6.0 × 10⁻⁶. 9 CFU / g).

[0073] Process:

[0074] 1. Mix solid manure with straw and crush it to a particle size of <5cm.

[0075] 2. Prepare materials according to the volume ratio of mixed solid materials: horse urine: straw = 45% : 35% : 20%, and add zeolite powder accounting for 6% of the total material weight, and dry mix evenly.

[0076] 3. Slowly pour in the waste pregnant mare urine while stirring, until the mixture reaches a moisture content of 55%.

[0077] 4. Inoculate with 0.1% compound microbial agent, build a pile (2m wide × 1.2m high), and cover with breathable felt.

[0078] Fermentation Management: On the third day, the temperature rises to 60℃, and the pile is turned for the first time. On the third day after the first turning, the temperature rises to 70℃. The high-temperature stage (≥55℃) is maintained for 15 days, with a total of 3 turnings. After that, the cooling stage begins. On the 40th day, the pile temperature drops to 29℃, the material turns dark brown, and has a slight earthy smell, indicating that fermentation is complete.

[0079] Example 3:

[0080] Raw materials: Waste pregnant mare urine after estrogen extraction (tested to be pH=8.9, EC=12.4mS / cm, estrone equivalent residue approximately 40μg / L), fresh sheep manure, horse manure, and cow manure (volume ratio 3:3:4), wheat straw fragments, 100-mesh clinoptilolite powder, and compound fermentation agent (composed of Bacillus subtilis). Bacillus subtilis Lateral spores of Bacillus brevis Brevibacillus laterite and brewing yeast Saccharomyces cerevisiae When formulated with a live bacteria ratio of 1:1:2, the total effective live bacteria count is approximately 5.8 × 10⁻⁶. 9 CFU / g).

[0081] Process:

[0082] 1. Mix solid manure with straw and crush it to a particle size of <5cm.

[0083] 2. Prepare materials according to the volume ratio of mixed solid materials: horse urine: straw = 70% : 25% : 5%, and add zeolite powder accounting for 7% of the total material weight, and dry mix evenly.

[0084] 3. Slowly pour in the waste pregnant mare urine while stirring, until the moisture content of the mixture reaches 53.5%.

[0085] 4. Inoculate with 0.1% compound microbial agent, build a pile (2m wide × 1.2m high), and cover with breathable felt.

[0086] Fermentation Management: On the third day, the temperature rises to 62℃, and the pile is turned for the first time. On the third day after the first turning, the temperature rises to 68℃. The high-temperature stage (≥55℃) is maintained for 12 days, with a total of 3 turnings. After that, the cooling stage begins. On the 38th day, the pile temperature drops to 29℃, the material turns dark brown, and has a slight earthy smell, indicating that fermentation is complete.

[0087] Example 4:

[0088] Raw materials: Waste pregnant mare urine after estrogen extraction (tested to be pH=8.9, EC=12.4mS / cm, estrone equivalent residue approximately 37μg / L), fresh sheep manure, horse manure, and cow manure (volume ratio 4:2:4), wheat straw fragments, 100-mesh zeolite powder, and compound fermentation agent (composed of Bacillus subtilis). Bacillus subtilis Lateral spores of Bacillus brevis Brevibacillus laterite and brewing yeast Saccharomyces cerevisiae When formulated with a live bacteria ratio of 1:1.5:2, the total effective live bacteria count is approximately 5.3 × 10⁻⁶. 9 CFU / g).

[0089] Process:

[0090] 1. Mix solid manure with straw and crush it to a particle size of <5cm.

[0091] 2. Prepare materials according to the volume ratio of mixed solid material: horse urine: straw = 50% : 30% : 20%, and add zeolite powder accounting for 8% of the total material weight, and dry mix evenly.

[0092] 3. Slowly pour in the waste pregnant mare urine while stirring, until the moisture content of the mixture reaches 52%.

[0093] 4. Inoculate with 0.1% compound microbial agent, build a pile (2m wide × 1.2m high), and cover with breathable felt.

[0094] Fermentation Management: On the third day, the temperature rises to 58℃, and the pile is turned for the first time. On the third day after the first turning, the temperature rises to 68℃. The high-temperature stage (≥55℃) is maintained for 20 days, with a total of 3 turnings. After that, the cooling stage begins. On the 42nd day, the pile temperature drops to 29℃, the material turns dark brown, and has a slight earthy smell, indicating that fermentation is complete.

[0095] Comparative Example 5 (Experiment on Optimization of Microbial Strains):

[0096] Compared to Example 2, the starter culture in Comparative Example 5 was replaced with a compound starter culture (purchased from Hubei Qiming Biotechnology Co., Ltd.) consisting of Bacillus jellyii, Aspergillus oryzae, and yeast in a ratio of 2:1.25:2, with a total effective viable count of approximately 6 × 10⁻⁶. 9 CFU / g, with all other raw materials and processes being exactly the same.

[0097] Fermentation Management: On day 3, the temperature rises to 50℃, and on day 6, it rises to 55℃, at which point the first turning of the pile is performed. On day 4 after the first turning, the temperature rises to 60℃. The high-temperature stage (≥55℃) is maintained for 30 days, with a total of 3 turnings. Afterward, a cooling stage begins. On day 56, the pile temperature drops to 29℃, the material turns dark brown, and has a slight earthy smell, indicating fermentation is complete; however, a small amount of straw, about 2cm in length, remains.

[0098] The results showed that after changing the fermenting agent, the highest fermentation temperature was only 60℃, 10℃ lower than in Example 2, and the fermentation time increased by 16 days. After fermentation, a small amount of straw with a length of 2cm in the comparative example was not completely fermented. The above comparative experiments demonstrate that the combination of fermentation strains "Bacillus subtilis + Bacillus laterosporus + Saccharomyces cerevisiae" in this invention has the best fermentation effect.

[0099] Comparative Example 6 (Segmented Fermentation Comparison):

[0100] Compared to Example 2, Comparative Example 6 used the same raw materials and process, the difference being that it did not employ segmented fermentation and the pile was not turned during fermentation. The temperature rose to 60°C on day 3 and reached a maximum of 65°C on day 6, maintaining a high-temperature phase (≥55°C) for 13 days. Afterwards, a cooling phase began, and by day 30, the pile temperature had dropped to 29°C. Most of the material was dark brown with a slight earthy smell, indicating fermentation was complete, but a significant amount of straw (2cm in length) remained unfermented.

[0101] The results showed that after the fermentation process was changed, the highest fermentation temperature was 65℃, which was 5℃ lower than that of Example 2. The fermentation time was shortened by 10 days, and there was still a lot of unfermented straw, indicating incomplete fermentation. The above comparative experiments demonstrate that the segmented fermentation process used in this invention achieves the best fermentation effect.

[0102] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A method for preparing water-saving organic fertilizer based on the co-fermentation of waste pregnant marma urine and multi-source manure, characterized in that, The discarded pregnant mare urine refers to the discarded pregnant mare urine after extracting and binding estrogen. Includes the following steps: S1. Material pretreatment: Collect the waste pregnant mare urine, sheep manure, horse manure, cow manure and high-carbon auxiliary materials respectively, mix the sheep manure, horse manure, cow manure and high-carbon auxiliary materials and crush them to a particle size of less than 3-5 cm to obtain mixed solid materials; S2. Mixing and Batching: The mixed solid material obtained in step S1, waste pregnant mare urine and zeolite powder are mixed in proportion, stirred and mixed evenly, and the initial moisture content of the pile is adjusted to 50%-60%; the waste pregnant mare urine is the only source of moisture for adjusting the moisture content of the pile. S3. Inoculation agent: Spray the compound microbial fermentation agent onto the mixture obtained in step S2; S4. Composting: The inoculated mixture is piled into a trapezoidal pile with a width of 1.5-2.5m and a height of 1.0-1.5m, and covered with a breathable material for fermentation; S5. Fermentation Management: Monitor the temperature of the fermentation pile and control the fermentation process into a high-temperature stage and a cooling stage; The high-temperature phase involves maintaining the reactor body temperature at 55-65℃ for 10-15 days; the cooling phase involves controlling the reactor body temperature at 29-50℃ for 15-25 days. When the temperature rises to 60-65℃, the pile is turned over for the first time. Subsequently, the pile is turned over every time the temperature exceeds 65℃. The entire fermentation process involves turning the pile 3-4 times. S6. Post-fermentation and evaluation: When the temperature of the compost pile drops to the ambient temperature and remains stable, and the material is dark brown and odorless, fermentation is complete, and the organic fertilizer is obtained.

2. The method according to claim 1, characterized in that, The volume ratio of the mixed solid material, horse urine, and high-carbon auxiliary material is (50%-70%):(20%-40%):(5%-15%), and the total volume percentage is 100%. In the mixed solid material, the volume ratio of sheep manure, horse manure and cow manure is (3-5):(2-4):(3-5).

3. The method according to claim 2, characterized in that, The volume ratio of the mixed solid material, horse urine and high-carbon auxiliary material is (50%-60%):(30%-40%):(10%-20%), and the total volume percentage is 100%.

4. The method according to claim 1, characterized in that, In step S2, the amount of zeolite powder added is 3%-8% of the total weight of the materials, and the particle size is 80-200 mesh. The total material refers to a mixture of mixed solid materials, horse urine, and high-carbon auxiliary materials.

5. The method according to claim 4, characterized in that, The amount of zeolite powder added is 5%-8%, and the particle size is 100 mesh.

6. The method according to claim 1, characterized in that, In step S3, the compound microbial fermentation agent contains Bacillus subtilis ( Bacillus subtilis ), Bacillus retroflexus ( Brevibacillus laterosporus ) and brewer's yeast ( Saccharomyces cerevisiae ); The agent is formulated with Bacillus subtilis, Bacillus laterosporus, and Saccharomyces cerevisiae in a viable cell ratio of (1-2):(1-1.5):(1-2); the total effective viable cell count of the agent is 5.0 × 10⁻⁶. 9 ~6.0×10 9 CFU / g, the inoculum amount is 0.1%-0.2% of the total weight of the mixture.

7. The method according to any one of claims 1-6, characterized in that, The EC conductivity of the waste pregnant mare urine ranges from 8 to 15 mS / cm, the pH value is 8.5 to 9.5, EC=12 to 15 mS / cm, and the estrone equivalent residue is 50 to 60 μg / L. The high-carbon auxiliary materials include crop straw.

8. The organic fertilizer prepared by any one of the methods described in claims 1-7, characterized in that, The organic fertilizer contains zeolite powder, and its natural conjugated estrogen activity equivalent, calculated as estrone, is less than 1 μg / kg.

9. Any of the following applications of the organic fertilizer according to claim 8: (1) Used for plant cultivation; (2) Used to improve soil.