Preparation method and application of traditional Chinese medicine fermentation liquor

Abstract

The invention relates to traditional Chinese medicine fermentation liquor, in particular to a preparation method and application of the traditional Chinese medicine fermentation liquor. Through the double-bacterium symbiotic fermentation technology, traditional Chinese medicine macromolecules are decomposed into micromolecules through fermentation, efficient absorption of the skin can be promoted, and by combining with the thuja old ointment, the antibacterial rate is increased, the skin barrier repairing function is achieved, and the thuja old ointment can be used for daily chemical products such as handmade soap, hand sanitizer, shower gel, shampoo and facial cleanser. Therefore, a multi-effect synergistic effect of cleaning, fixing hair, preventing hair loss and nourishing skin is realized.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of daily chemical cleaning, and particularly relates to a preparation method and application of a traditional Chinese medicine fermentation liquor. BACKGROUND

[0002] In recent years, the market of daily cleaning products has developed very rapidly. Daily chemical products prepared by taking traditional Chinese medicine as a natural raw material have become a main development trend due to obvious effects and small side effects.

[0003] Daily chemical products, such as handmade soap, hand sanitizer, shower gel, shampoo, and facial cleanser, mostly take Chinese herbal medicine as a natural raw material, adopt a cold process, and realize cleaning function through saponification of oil and fat. However, there are problems such as the need for multiple grinding, long curing period, low efficiency, and low transdermal absorption rate of active ingredients of traditional Chinese medicine due to large molecular weight. In addition, traditional daily chemical products with traditional Chinese medicine components have single effects and cannot meet the multiple needs of modern consumers. Therefore, it is necessary to develop a daily chemical product with small-molecule traditional Chinese medicine components, high transdermal rate, and multiple effects. SUMMARY

[0004] The application provides a preparation method and application of a traditional Chinese medicine fermentation liquor, which is obtained by fermenting a traditional Chinese medicine compound with double-strain bacteria. The yeast and lactic acid bacteria can be synergistically combined to reduce the molecular weight of active ingredients of traditional Chinese medicine, realize the purpose of fully fermenting complex traditional Chinese medicine components, and further improve the transdermal absorption rate. In addition, multiple traditional Chinese medicinal materials are used in combination, and functional additives are added, so that the effects of the medicinal materials and the bacteriostatic and repair effects of the functional additives can be fully exerted, thereby realizing the synergistic effects of cleaning, hair fixing, hair loss prevention, and skin care.

[0005] In the first aspect, the application provides a traditional Chinese medicine fermentation liquor, which is obtained by fermenting a traditional Chinese medicine compound with double-strain bacteria. The double-strain bacteria include yeast and lactic acid bacteria. The yeast can degrade polysaccharides to decompose large-molecule active substances in traditional Chinese medicinal materials into small-molecule active ingredients. The lactic acid bacteria can change the acid-base property of the traditional Chinese medicine compound, so that the large molecules of traditional Chinese medicine are hydrolyzed to generate more easily absorbed active ingredients. The yeast and lactic acid bacteria can be synergistically combined to further decompose large-molecule substances such as sugars and proteins in the traditional Chinese medicine compound to produce more active small-molecule substances such as small-molecule peptides, thereby reducing the molecular weight of active ingredients of traditional Chinese medicine, realizing the purpose of fully fermenting complex traditional Chinese medicine components, and further improving the transdermal absorption rate.

[0006] In some embodiments, the volume ratio of the yeast and the lactic acid bacteria is 1: (1.2-1.8). When the condition is met, the molecular weight of the active ingredients of traditional Chinese medicine can be reduced, thereby realizing the purpose of fully fermenting complex traditional Chinese medicine components, and further improving the transdermal absorption rate.

[0007] In some embodiments, the number average molecular weight of the traditional Chinese medicine fermentation liquor is less than or equal to 500 Da. When this condition is met, the purpose of fully fermenting complex traditional Chinese medicine components can be achieved, thereby improving the transdermal absorption rate.

[0008] In some embodiments, the traditional Chinese medicine compound includes the following components by weight: Gleditsia sinensis 40-50 parts, Sapindus 30-40 parts, Biota orientalis 2-3 parts, Sophora flavescens 1-2 parts, Astragalus membranaceus 2-3 parts, Angelica sinensis 2-3 parts, Rehmannia glutinosa 1-2 parts, Polygonum multiflorum 2-3 parts. When this condition is met, by compounding multiple traditional Chinese medicines, the efficacy of the medicinal materials can be fully utilized through the double-bacterial fermentation process, achieving the effect of cleaning, hair fixing, hair loss prevention, and skin nourishment.

[0009] In some embodiments, the present application provides a preparation method of a traditional Chinese medicine fermentation liquor, including the following steps: Step S1: mixing yeast bacteria with an inoculum of 1-3% wt and lactic acid bacteria with an inoculum of 2-5% wt, activating at 30-37°C for 12-32 hours to obtain a double-bacterial activation liquid; Step S2: crushing the medicinal materials in the traditional Chinese medicine compound, then mixing with sterile water at a ratio of g:ml = 1:3-7, sterilizing at a pressure of 10-50 MPa and a temperature of 105-120°C, and then cooling to 40°C to obtain a cooled mixed liquid; Step S3: adding the cooled mixed liquid to the double-bacterial activation liquid, adjusting the pH to 4.5-5.5, then fermenting at 28-42°C for 24-72 hours, and then obtaining the traditional Chinese medicine fermentation liquor after separation.

[0010] Based on the above preparation method, by using yeast bacteria and lactic acid bacteria, the molecular weight of the active components of traditional Chinese medicine can be effectively reduced, the purpose of fully fermenting complex traditional Chinese medicine components can be achieved, thereby improving the transdermal absorption rate. In addition, the traditional Chinese medicine compound uses multiple medicinal materials, thereby achieving the effect of cleaning, hair fixing, hair loss prevention, and skin nourishment.

[0011] In some embodiments, the yeast bacteria include Brettanomyces, and the volume ratio of Brettanomyces to the volume of the yeast bacteria is 30-45%. When this condition is met, the molecular weight of the active components of traditional Chinese medicine can be reduced, thereby achieving the purpose of fully fermenting complex traditional Chinese medicine components, and improving the transdermal absorption rate.

[0012] In a second aspect, the present application provides a use of a traditional Chinese medicine fermentation liquor and a preparation method in daily chemical products, including handmade soap, hand sanitizer, shower gel, shampoo, and facial cleanser. Based on the above traditional Chinese medicine fermentation liquor and preparation method, the developed daily chemical products have high transdermal absorption rate, cleaning, hair fixing, hair loss prevention, and skin nourishment effects.

[0013] In some embodiments, a handmade soap comprises a traditional Chinese medicine fermentation liquor and a soap base, wherein the traditional Chinese medicine fermentation liquor accounts for 25-35% by volume; the soap base comprises the following components: coconut oil 20-25%, palm oil 10-15%, olive fruit oil 5-10%, jojoba seed oil 2-5%, and guava fruit oil 1-3%. When the condition is met, the various components of the soap base can cooperate with each other, thereby achieving efficient cleaning and realizing the synergistic effects of cleaning, hair fixing, hair loss prevention, and skin care.

[0014] In some embodiments, the pH of the handmade soap is 7.0-8.5; the handmade soap further comprises a functional additive; the functional additive comprises Thuja old ointment 1-3% by volume; the preparation method of the Thuja old ointment comprises the following steps: subjecting Thuja to steam distillation at 100-105℃ for 3-10h, and then obtaining the Thuja old ointment by separating and processing the distillation product. When the condition is met, the bacteriostatic and repairing effects of the Thuja old ointment can be fully exerted, thereby making the developed handmade soap have the effects of high transdermal absorption rate, cleaning, hair fixing, hair loss prevention, and skin care.

[0015] In some embodiments, a handmade soap preparation method comprises the following steps: heating and melting a soap base at 40-50℃, then adding a traditional Chinese medicine fermentation liquor and a functional additive, stirring to an emulsified state, molding, and curing for 25-35 days after solidification. When the condition is met, the developed handmade soap has a short curing period and has the effects of high transdermal absorption rate, cleaning, hair fixing, hair loss prevention, and skin care. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 A flowchart of a double-bacteria fermentation process provided in Embodiment 1 of the present application. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical solutions and advantages of the present application clearer and more apparent, the present application is further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and do not limit the present application.

[0018] Embodiment 1 A preparation method of a traditional Chinese medicine fermentation liquor 1. Strain activation The yeast strain is cultured in YPD medium at 30℃ for 24h to obtain activated yeast strain, wherein the yeast strain comprises Saccharomyces boulardii, which accounts for 35% by volume, and the rest is Rhodotorula.

[0019] The Lactobacillus plantarum is cultured in MRS medium at 37℃ for 24h to obtain activated lactic acid bacteria.

[0020] The Saccharomyces boulardii CNCMI-745 used in this application was purchased from Biocodex, France; Rhodotorula rubra CICC 32622 and Lactiplantibacillus plantarum CICC 20982 were both purchased from the China Industrial Microbial Culture Collection Center.

[0021] 2. Cooling mixture of traditional Chinese medicine compound Prepared Chinese herbal compound formula: 50g of soapberry, 40g of soapberry, 2g of arborvitae leaf, 1g of sophora flavescens, 2g of astragalus membranaceus, 2g of angelica sinensis, 1g of rehmannia glutinosa, and 2g of polygonum multiflorum. The components are washed, dried, and ground into powder using a 35,000 rpm pulverizer. The powder is then passed through an 80-mesh sieve. The powdered components are immersed in sterile water and mixed at a ratio of g:ml = 1:5. The mixture is then sterilized in a sterilization tank at a pressure of 40 MPa and a temperature of 110°C. Under these temperature and pressure conditions, the cell walls of the components are broken down, and the mixture is then cooled to 40°C to obtain a cooled mixture.

[0022] The effects of each component in the traditional Chinese medicine compound are as follows: Soapberry: Removes dandruff, darkens hair, prevents hair loss, promotes hair regeneration, clears heat and invigorates blood circulation, removes dirt, removes oil and relieves itching.

[0023] Soapberry: Clears heat and detoxifies, reduces swelling and relieves pain, kills parasites and relieves itching, cools the blood and stops bleeding, and inhibits dandruff production.

[0024] Arborvitae leaves: cool the blood and stop bleeding, resolve phlegm and stop cough, promote hair growth and darken hair; mainly used to treat various bleeding disorders, lung heat cough, burns and hair loss due to blood heat.

[0025] Sophora flavescens has the effects of clearing heat and drying dampness, inhibiting bacteria and relieving itching, promoting blood circulation and removing blood stasis, and promoting diuresis.

[0026] Astragalus has the effects of strengthening the exterior and stopping sweating, promoting diuresis and reducing swelling, generating fluids and nourishing blood, promoting blood circulation and relieving pain, promoting the elimination of toxins and pus, and promoting wound healing and tissue regeneration.

[0027] Angelica sinensis: It nourishes and invigorates blood circulation, moistens dryness and lubricates the intestines, dilates capillaries in the scalp, strengthens hair, revitalizes the scalp, and prevents hair loss.

[0028] Rehmannia glutinosa: Clears heat and generates fluids, cools blood and stops bleeding. It is used for febrile diseases that damage yin, red tongue and thirst, fluid depletion and constipation, yin deficiency fever, bone steaming fever, internal heat and thirst, and enhances the body's immunity and hematopoietic function.

[0029] Polygonum multiflorum (He Shou Wu): Nourishes essence and blood, treats malaria, detoxifies, and moistens the intestines to relieve constipation; it also nourishes essence and blood, darkens hair, strengthens muscles and bones, and reduces turbidity and lipids. It contains nutrients such as lecithin, which nourishes blood and dispels wind, regulates nerve and endocrine functions, nourishes hair roots, and promotes the production of melanin, making hair darker. Simultaneously, Polygonum multiflorum also contains emodin and a large amount of starch. After starch hydrolysis, it can produce glucose, which has excellent hair-moisturizing effects, making it an ideal traditional Chinese medicine ingredient for formulating hair conditioning products.

[0030] 3. Traditional Chinese medicine fermentation liquid The traditional Chinese medicine compound cooling mixture was mixed with 2% wt inoculation of yeast activation solution and 3% wt inoculation of lactic acid bacteria activation solution, wherein the volume ratio of yeast to lactic acid bacteria was 1:1.5. Then, it was fermented in a fermentation tank at pH 5.0 and temperature 37℃ for 72 hours, and then separated by centrifugation at 8000 rpm for 10 minutes. The supernatant was collected to obtain the traditional Chinese medicine fermentation broth.

[0031] A method for preparing handmade soap including fermented Chinese herbal medicine liquid 4. Preparation of old cypress oil The cliff cypress was placed in a distillation pot with a mass ratio of cliff cypress to water of 1:30. It was distilled with steam at 100℃ for 8 hours. The distillation product was then separated to obtain old cliff cypress oil.

[0032] The effects of cliff cypress: It has antibacterial, antioxidant, antitumor, blood pressure lowering, anthelmintic, anti-inflammatory, diuretic, expectorant and health-strengthening effects.

[0033] 5. Soap base formula Melt 1200mL of coconut oil, 700mL of palm oil, 400mL of olive fruit oil, 200mL of jojoba seed oil, and 100mL of dogtooth rosehip oil at 45℃. Then add 1800mL of traditional Chinese medicine fermentation liquid and 180mL of functional additive, old cypress oil. Stir by hand until emulsified, pour into a suitable mold, and solidify at 30℃. Then mature for 30 days until the pH value stabilizes at 7.0 to obtain a handmade soap containing traditional Chinese medicine fermentation liquid.

[0034] Comparative Example 1 The only difference between this comparative example of traditional Chinese medicine fermentation liquid and Example 1 is that: in the strain activation, only yeast is activated and in the traditional Chinese medicine fermentation liquid, only yeast activation liquid is added.

[0035] Comparative Example 2 The only difference between this comparative example of traditional Chinese medicine fermentation broth and Example 1 is that only *Lactobacillus plantarum* was activated during the microbial activation process, and only lactic acid bacteria activation broth was added to the traditional Chinese medicine fermentation broth.

[0036] Example 2 The difference between the traditional Chinese medicine fermentation liquid in this embodiment and that in Example 1 is only that the traditional Chinese medicine compound cooling mixture is mixed with 2.5%wt inoculation amount of yeast activation liquid and 2.5%wt inoculation amount of lactic acid bacteria activation liquid, wherein the volume ratio of yeast to lactic acid bacteria is 1:1.

[0037] Example 3 The yeast includes 100% red yeast by volume.

[0038] Example 4 The difference between the traditional Chinese medicine fermentation liquid in this embodiment and that in Example 1 is that the traditional Chinese medicine compound cooling mixture is mixed with 4%wt yeast activation liquid and 6%wt lactic acid bacteria activation liquid.

[0039] Example 5 The only difference between the fermented Chinese medicine liquid in this embodiment and that in Example 1 is that no functional additives are added to the soap base formula.

[0040] Test case The number-average molecular weight, transdermal absorption rate, antibacterial rate, and hair follicle density enhancement rate of each example and comparative example were determined. The number-average molecular weight was determined by high-performance gel permeation chromatography (HPSEC), and the number-average molecular weight was calculated based on the retention time. The instrument used was an Agilent 1260 chromatograph and a TSKgel G2000SWXL column (300mm×7.8mm). The standards used were dextran (100, 500, 1000 Da), the mobile phase was 0.1 mol / L phosphate buffer (pH 7.0), and the flow rate was 0.8 mL / min. Transdermal absorption rate was determined using the Franz diffusion cell method (ex vivo rat skin model). The cumulative transdermal absorption was measured over 12 hours using a fermentation broth containing 0.1% sodium fluorescein tracer. The transdermal absorption rate was calculated as follows: Transdermal absorption rate (%) = (cumulative transdermal absorption over 12 hours × receiving cell volume) / initial drug amount in the supply cell × 100%. The receiving solution used was PBS buffer (pH 7.4, containing 0.5% Tween-80), and the mixture was stirred at 37℃ and 600 rpm. Antibacterial activity was determined using the plate count method provided in national standard GB / T 20944.3-2008 for Staphylococcus aureus (ATCC 6538) and Malassezia (ATCC 14521). The bacterial concentration was 1 × 10⁻⁶. 6 The incubation period was 24 hours at 37℃ with a concentration of CFU / mL. The inhibition rate was calculated using the following formula: Inhibition rate (%) = (Control group CFU - Experimental group CFU) / Control group CFU × 100%. The hair follicle density improvement rate was determined by selecting 30 patients with a hair follicle density <100 follicles / cm². 2Volunteers with mild hair loss were randomly assigned to an experimental group and a control group. The experimental group used handmade soap containing fermented liquid, while the control group used basic soap base. Before use (D0) and 28 days after use (D28), dermoscopic images (×200) were taken of the top 1cm of the scalp. 2 The number of hair follicles in the area; the hair follicle density improvement rate is calculated using the following formula: Hair follicle density improvement rate (%) = (D28 - D0) / D0 × 100%

[0041] As shown in the table above, the handmade soap developed in this application has the effects of small molecular weight of active ingredients from traditional Chinese medicine, high transdermal absorption rate, cleansing, hair strengthening, and anti-hair loss. When using a dual-strain synergistic fermentation of traditional Chinese medicine compound, the fermented liquid of traditional Chinese medicine can have the effect of small molecular weight and high transdermal absorption rate. In particular, when using handmade soap made from fermented liquid of traditional Chinese medicine with added functional additives, the antibacterial rate and hair follicle density can be significantly improved. Especially, when the inoculation amount and volume ratio of yeast and lactic acid bacteria in the dual strains are controlled to meet the following: 2% wt of yeast activation liquid and 3% wt of lactic acid bacteria activation liquid are mixed, wherein the volume ratio of yeast and lactic acid bacteria is 1:1.5, the synergistic effect of the dual strains can be fully exerted, and the nutrient competition caused by excessively high strain concentration can be avoided. Thus, the developed handmade soap can have the effects of small molecular weight of active ingredients from traditional Chinese medicine, high transdermal absorption rate, cleansing, hair strengthening, and anti-hair loss.

[0042] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the principles of this application should be included within the protection scope of this application.

Claims

1. A fermentation liquid for traditional Chinese medicine, characterized in that, The fermented liquid of traditional Chinese medicine is obtained by fermenting a compound traditional Chinese medicine with two strains of bacteria, namely yeast and lactic acid bacteria.

2. The traditional Chinese medicine fermentation liquid according to claim 1, characterized in that, The volume ratio of the yeast to the lactic acid bacteria is 1:(1.2~1.8).

3. The traditional Chinese medicine fermentation liquid according to claim 1 or 2, characterized in that, The number-average molecular weight of the fermented Chinese medicine broth is ≤500 Da.

4. The traditional Chinese medicine fermentation liquid according to claim 1 or 2, characterized in that, The traditional Chinese medicine compound includes the following components by weight: 40-50 parts of Gleditsia sinensis, 30-40 parts of Sapindus mukorossi, 2-3 parts of Platycladus orientalis leaves, 1-2 parts of Sophora flavescens, 2-3 parts of Astragalus membranaceus, 2-3 parts of Angelica sinensis, 1-2 parts of Rehmannia glutinosa, and 2-3 parts of Polygonum multiflorum.

5. The method for preparing traditional Chinese medicine fermentation broth according to any one of claims 1 to 4, characterized in that, Includes the following steps: Step S1: Mix 1-3%wt of yeast and 2-5%wt of lactic acid bacteria, and activate at 30-37℃ for 12-32 hours to obtain a dual-bacterial activation solution; Step S2: Crush the medicinal materials in the Chinese herbal compound, then mix them with sterile water at a ratio of g:ml = 1:3~7, sterilize them at a pressure of 10~50MPa and a temperature of 105~120℃, and then cool them to 40℃ to obtain a cooled mixture; Step S3: Add the cooling mixture to the dual-bacterial activation solution, adjust the pH to 4.5~5.5, and then ferment at 28~42℃ for 24~72 hours. After fermentation is completed, separate the fermented Chinese medicine liquid.

6. The preparation method according to claim 5, characterized in that, The yeast includes Saccharomyces boulardii, and the volume percentage of Saccharomyces boulardii is 30-45% based on the volume of the yeast.

7. The application of a traditional Chinese medicine fermentation liquid in the preparation of daily chemical products, characterized in that, The daily chemical products include handmade soap, hand sanitizer, shower gel, shampoo, and facial cleanser; The traditional Chinese medicine fermentation liquid includes the traditional Chinese medicine fermentation liquid according to any one of claims 1 to 4, or the traditional Chinese medicine fermentation liquid prepared by the preparation method according to any one of claims 5 to 6.

8. A handmade soap, characterized in that, The handmade soap comprises a traditional Chinese medicine fermentation liquid and a soap base, wherein the traditional Chinese medicine fermentation liquid comprises 25-35% by volume; and the soap base comprises the following components: coconut oil 20-25%, palm oil 10-15%, olive fruit oil 5-10%, jojoba seed oil 2-5%, and rosehip oil 1-3%. The traditional Chinese medicine fermentation liquid includes the traditional Chinese medicine fermentation liquid according to any one of claims 1 to 4, or the traditional Chinese medicine fermentation liquid prepared by the preparation method according to any one of claims 5 to 6.

9. The handmade soap according to claim 8, characterized in that, The pH of the handmade soap is 7.0~8.5; The handmade soap also includes functional additives; the functional additives include 1-3% old cypress oil. The preparation method of the old cypress oil includes: steam distilling cypress at 100~105℃ for 3~10h, and then separating the distillation product to obtain the old cypress oil.

10. The method for preparing handmade soap according to claim 8 or 9, characterized in that, Includes the following steps: The soap base is heated to 40-50°C and melted. Then, the fermented Chinese medicine liquid and the functional additives are added and stirred until emulsified. The mixture is poured into a mold, solidified, and aged for 25-35 days to obtain the final product.

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