Essence helpful for activating cell viability and preparation method thereof
This serum, formulated through the synergistic effects of plant extract fermentation and microbial lysate, addresses skin problems caused by declining cell activity, enhancing cell metabolism and repairing the skin barrier to improve overall skin condition.
Patent Information
- Application Number
- CN202511566485.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-30
- Publication Date
- 2026-02-10
AI Technical Summary
As a result of both external and internal factors, declining cell activity leads to dry and rough skin, and in severe cases, damage to the skin barrier and sensitivity. Existing medical aesthetic methods are expensive and highly irritating to the skin, while ordinary skincare products have limited effectiveness.
An essence is prepared by synergistic action of plant extract fermentation and microbial lysate, containing a cell activity promoting composition to promote cell metabolism and skin barrier repair, including the combined use of banana fruit, myrothamnus flabellifolia, sea fennel, palm leaf extract and microbial fermentation lysate.
Serums can promote cell metabolism, enhance skin regeneration, repair the skin barrier, improve skin condition, provide a variety of nutrients, activate cell activity, and strengthen the skin barrier function.
Smart Images
Figure SMS_1 
Figure SMS_2
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cosmetic technology and relates to an essence that helps activate cell activity and its preparation method. Background Technology
[0002] External factors such as ultraviolet radiation and environmental pollution, unhealthy lifestyle habits such as staying up late and high-sugar diets, and internal factors such as aging and hormonal changes can all lead to a decline in cell activity and a slowdown in epidermal cell metabolism, resulting in dry, rough skin. In severe cases, it can even cause skin barrier damage, redness, and sensitivity. Currently, the solutions are either cosmetic procedures or skincare products. However, cosmetic procedures are expensive, can be irritating to the skin, and have potential side effects. Therefore, gentler, functional skincare products have become the preferred choice for consumers. Summary of the Invention
[0003] The purpose of this invention is to provide an essence that helps activate cell activity and its preparation method. The essence prepared by this invention, which helps activate cell activity, can provide the skin with a variety of nutrients through the synergistic effect of plant extract fermentation and microbial lysate products, thereby promoting cell metabolism, assisting in activating cell activity, and effectively restoring and strengthening the skin barrier, thus improving skin condition.
[0004] The objective of this invention can be achieved through the following technical solutions: A serum that helps activate cell activity, the serum comprising, by weight, 5-9 parts cell activity promoting composition, 6-8 parts glycerin, 3-7 parts butylene glycol, 1-3 parts 1,2-pentanediol, 1-4 parts octyl polymethylsiloxane, 1-2 parts polydimethylsiloxane, 0.8-1.2 parts hydrogenated lecithin, 0.02-0.08 parts tetrahydropiperine, 0.8-1.2 parts octyldodecanol, 1.5- 2.5 parts trehalose, 0.2-0.4 parts tetrahydromethylpyrimidine carboxylic acid, 0.1-0.3 parts fibronectin, 0.01-0.05 parts acetyl hexapeptide-8, 0.05-0.15 parts sodium heparin, 0.2-0.8 parts allantoin, 0.5-1.5 parts panthenol, 0.1-0.5 parts sodium hyaluronate, 0.05-0.15 parts xanthan gum, 0.2-0.6 parts ethylhexylglycerin, and 60-70 parts deionized water.
[0005] Furthermore, the cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0006] Further, the mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 0.8-1.2:0.4-0.6:1.9-2.3:0.5-1.1:0.04-0.06:0.0008-0.0012.
[0007] Further, the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract in step A1 were prepared according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through a 60-100 mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 72-76wt% ethanol solution, reflux at 81-83℃ for 1.5-2.5h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol odor to obtain banana fruit ethanol extract, then add cellulase and pectinase, adjust the pH to 4.1-4.5, enzymatically hydrolyze at 48-52℃ for 3-4h, inactivate, centrifuge, filter, and obtain banana fruit extract; B3. Mix the crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree with 80-86wt% ethanol solution, reflux at 81-83℃ for 1.5-2.5h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol odor, and obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Add cellulase to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, adjust the pH to 4.8-5.2, and enzymatically hydrolyze each extract at 35-39℃ for 2-3 hours. After inactivation, centrifuge and filter to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
[0008] Further, in step B2, the mass ratio of the crushed banana fruit to the 72-76 wt% ethanol solution is 1:15-16; the mass ratio of the banana fruit ethanol extract, cellulase, and pectinase is 1:0.012-0.018:0.01-0.014.
[0009] Further, in step B3, the mass ratio of the crushed Myrothamnus fruticosa to the 80-86 wt% ethanol solution is 1:14-15; the mass ratio of the crushed Sea Fennel to the 80-86 wt% ethanol solution is 1:12.7-13.5; and the mass ratio of the crushed Palm Leaf Tree to the 80-86 wt% ethanol solution is 1:14.7-15.3.
[0010] Further, in step B4, the mass ratio of Myrothamnus rotundifolia alcohol extract to cellulase is 1:0.02-0.026; the mass ratio of Amygdalina scabra alcohol extract to cellulase is 1:0.016-0.02; and the mass ratio of Palmarosa scabra alcohol extract to cellulase is 1:0.02-0.024.
[0011] Further, the inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 7-9 × 10⁻⁶. 8 CFU / mL; the fermentation refers to fermentation at 35-39℃ for 68-76h, with the pH controlled at 6.4-6.6 during fermentation.
[0012] Further, in step A3, the mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water is 5-5.2:1.9-2.3:0.8-1.2:0.2-0.3:0.25-0.35:10-12; the stirring refers to stirring at 2500-3500 rpm for 20-30 minutes.
[0013] Furthermore, the method for preparing the serum that helps activate cell activity includes the following steps: C1. Mix octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol, and stir in a water bath at 73-77°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 78-82°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 8000-12000 rpm for 5-7 minutes, and then cool to 44-46℃ to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 200-300 rpm for 15-25 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5-5.4, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.
[0014] The beneficial effects of this invention are: This invention provides an essence that helps activate cell activity. Through the synergistic effect of plant extract fermentation and microbial lysate, it enhances cell activity. The fermented plant extracts are more easily absorbed by the skin, providing various nutrients. In addition, banana fruit extract promotes cell proliferation and differentiation, enhancing skin regeneration; Myrothamnus flabellifolia extract stimulates cell metabolism, promotes cell regeneration, repairs damaged skin, and promotes lipid synthesis in the stratum corneum; sea fennel extract promotes epidermal cell renewal and strengthens stratum corneum function, thus promoting cell differentiation and repairing the skin barrier; palm-leaf tree extract promotes blood circulation and enhances the transdermal absorption of other ingredients; Lactobacillus fermentation lysate and Pichia pastoris fermentation lysate contain small molecules such as nucleotides and amino acids, which can activate the cell's own repair mechanism and enhance the activity of intracellular antioxidant enzymes, reducing free radical damage to cells. Through the synergistic effect of plant extract fermentation and microbial lysate, it provides various nutrients to the skin, thereby promoting cell metabolism, assisting in activating cell activity, effectively restoring and strengthening the skin barrier, and improving skin condition. Detailed Implementation
[0015] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.
[0016] In all embodiments and comparative examples of this invention, the fermentation lysate of Lactobacillus and the fermentation lysate filtrate of Pichia pastoris were purchased from Guangzhou Huayang Biotechnology Co., Ltd.; cellulase, pectinase, yeast extract, Lactobacillus plantarum, and sodium hyaluronate were purchased from Zhejiang Yicun Biotechnology Co., Ltd.
[0017] Example 1 An essence that helps activate cell activity, the essence comprising, by weight, 5 parts cell activity promoting composition, 6 parts glycerin, 3 parts butylene glycol, 1 part 1,2-pentanediol, 1 part octyl polymethylsiloxane, 1 part polydimethylsiloxane, 0.8 parts hydrogenated lecithin, 0.02 parts tetrahydropiperine, 0.8 parts octyldodecanol, 1.5 parts trehalose, 0.2 parts tetrahydromethylpyrimidine carboxylic acid, 0.1 parts fibronectin, 0.01 parts acetyl hexapeptide-8, 0.05 parts sodium heparin, 0.2 parts allantoin, 0.5 parts panthenol, 0.1 parts sodium hyaluronate, 0.05 parts xanthan gum, 0.2 parts ethylhexylglycerin, and 60 parts deionized water.
[0018] The cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0019] The mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 0.8:0.4:1.9:0.5:0.04:0.0008.
[0020] Step A1 involves preparing the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through a 60-mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 72wt% ethanol solution, reflux at 81℃ for 1.5h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell to obtain banana fruit ethanol extract, add cellulase and pectinase, adjust the pH to 4.1, enzymatically hydrolyze at 48℃ for 3h, inactivate, centrifuge, filter, and obtain banana fruit extract. B3. The crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree were respectively mixed with 80wt% ethanol solution and refluxed at 81℃ for 1.5h. After repeating this process several times, the extracts were combined and concentrated under reduced pressure until there was no alcohol odor, to obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Cellulase was added to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, and the pH was adjusted to 4.8. Each extract was enzymatically hydrolyzed at 35°C for 2 hours, inactivated, centrifuged, and filtered to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
[0021] In step B2, the mass ratio of the crushed banana fruit to the 72wt% ethanol solution is 1:15; the mass ratio of the banana fruit ethanol extract, cellulase, and pectinase is 1:0.012:0.01.
[0022] The mass ratio of the crushed Myrothamnus fruticosa to the 80wt% ethanol solution in step B3 is 1:14; the mass ratio of the crushed Sea Fennel to the 80wt% ethanol solution is 1:12.7; and the mass ratio of the crushed Palm Leaf Tree to the 80wt% ethanol solution is 1:14.7.
[0023] In step B4, the mass ratio of Myrothamnus flavescentis extract to cellulase is 1:0.02; the mass ratio of sea fennel extract to cellulase is 1:0.016; and the mass ratio of palm-leaf extract to cellulase is 1:0.02.
[0024] The inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 7 × 10⁻⁶. 8 CFU / mL; fermentation refers to fermentation at 35℃ for 68 hours, with the pH controlled at 6.4 during fermentation.
[0025] The mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water in step A3 is 5:1.9:0.8:0.2:0.25:10; stirring refers to stirring at 2500 rpm for 20 minutes.
[0026] The method for preparing the serum that helps activate cell activity includes the following steps: C1. Octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol are mixed and stirred in a water bath at 73°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 78°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 8000 rpm for 5 minutes, and then cool to 44℃ to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 200 rpm for 15 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.
[0027] Example 2 An essence that helps activate cell activity, the essence comprising, by weight, 6 parts cell activity promoting composition, 6.5 parts glycerin, 4 parts butylene glycol, 1.5 parts 1,2-pentanediol, 1.8 parts octyl polymethylsiloxane, 1.2 parts polydimethylsiloxane, 0.9 parts hydrogenated lecithin, 0.04 parts tetrahydropiperine, 0.9 parts octyldodecanol, 1.7 parts trehalose, 0.25 parts tetrahydromethylpyrimidine carboxylic acid, 0.15 parts fibronectin, 0.02 parts acetyl hexapeptide-8, 0.08 parts sodium heparin, 0.4 parts allantoin, 0.8 parts panthenol, 0.2 parts sodium hyaluronate, 0.08 parts xanthan gum, 0.3 parts ethylhexylglycerin, and 62 parts deionized water.
[0028] The cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0029] The mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 0.9:0.45:2:0.65:0.04:0.0009.
[0030] Step A1 involves preparing the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through a 60-mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 73wt% ethanol solution, reflux at 81℃ for 1.8h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell to obtain banana fruit ethanol extract, add cellulase and pectinase, adjust the pH to 4.2, enzymatically hydrolyze at 49℃ for 3.2h, inactivate, centrifuge, filter to obtain banana fruit extract. B3. The crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree were respectively mixed with 82wt% ethanol solution and refluxed at 81℃ for 1.8h. After repeating this process several times, the extracts were combined and concentrated under reduced pressure until there was no alcohol odor, to obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Cellulase was added to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, and the pH was adjusted to 4.9. The extracts were then enzymatically hydrolyzed at 36°C for 2.2 h, inactivated, centrifuged, and filtered to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
[0031] The mass ratio of the crushed banana fruit and the 73wt% ethanol solution in step B2 is 1:15.3; the mass ratio of the banana fruit ethanol extract, cellulase, and pectinase is 1:0.014:0.011.
[0032] The mass ratio of the crushed Myrothamnus fruticosa to the 82wt% ethanol solution in step B3 is 1:14.2; the mass ratio of the crushed Sea Fennel to the 82wt% ethanol solution is 1:12.9; and the mass ratio of the crushed Palm Leaf Tree to the 82wt% ethanol solution is 1:14.9.
[0033] In step B4, the mass ratio of Myrothamnus flabellifolia extract to cellulase is 1:0.022; the mass ratio of sea fennel extract to cellulase is 1:0.017; and the mass ratio of palm-leaf extract to cellulase is 1:0.021.
[0034] The inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 7.5 × 10⁻⁶. 8 CFU / mL; fermentation refers to fermentation at 36℃ for 70 hours, with the pH controlled at 6.4 during fermentation.
[0035] The mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water in step A3 is 5.05:2:0.9:0.22:0.28:10.5; stirring refers to stirring at 2500 rpm for 22 minutes.
[0036] The method for preparing the serum that helps activate cell activity includes the following steps: C1. Octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol are mixed and stirred in a water bath at 74°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 79°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 9000 rpm for 5 minutes, and then cool to 44℃ to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 200 rpm for 18 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5.1, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.
[0037] Example 3 An essence that helps activate cell activity, the essence comprising, by weight, 7 parts cell activity promoting composition, 7 parts glycerin, 5 parts butylene glycol, 2 parts 1,2-pentanediol, 2.5 parts octyl polymethylsiloxane, 1.5 parts polydimethylsiloxane, 1 part hydrogenated lecithin, 0.05 parts tetrahydropiperine, 1 part octyldodecanol, 2 parts trehalose, 0.3 parts tetrahydromethylpyrimidine carboxylic acid, 0.2 parts fibronectin, 0.03 parts acetyl hexapeptide-8, 0.1 parts sodium heparin, 0.5 parts allantoin, 1 part panthenol, 0.3 parts sodium hyaluronate, 0.1 parts xanthan gum, 0.4 parts ethylhexylglycerin, and 65 parts deionized water.
[0038] The cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0039] The mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 1:0.5:2.1:0.8:0.05:0.001.
[0040] Step A1 involves preparing the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through an 80-mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 74wt% ethanol solution, reflux at 82℃ for 2h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell to obtain banana fruit ethanol extract, add cellulase and pectinase, adjust the pH to 4.3, enzymatically hydrolyze at 50℃ for 3.5h, inactivate, centrifuge, filter to obtain banana fruit extract. B3. Mix the crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree with 83wt% ethanol solution, reflux at 82℃ for 2h, repeat several times, combine the extracts, and concentrate under reduced pressure until there is no alcohol taste to obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Cellulase was added to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, and the pH was adjusted to 5. The extracts were then enzymatically hydrolyzed at 37°C for 2.5 h, inactivated, centrifuged, and filtered to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
[0041] The mass ratio of the crushed banana fruit and the 74wt% ethanol solution in step B2 is 1:15.5; the mass ratio of the banana fruit ethanol extract, cellulase, and pectinase is 1:0.015:0.012.
[0042] The mass ratio of the crushed Myrothamnus fruticosa to the 83wt% ethanol solution in step B3 is 1:14.5; the mass ratio of the crushed Sea Fennel to the 83wt% ethanol solution is 1:13.1; and the mass ratio of the crushed Palm Leaf Tree to the 83wt% ethanol solution is 1:15.
[0043] In step B4, the mass ratio of Myrothamnus flabellifolia extract to cellulase is 1:0.023; the mass ratio of sea fennel extract to cellulase is 1:0.018; and the mass ratio of palm-leaf extract to cellulase is 1:0.022.
[0044] The inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 8 × 10⁻⁶. 8 CFU / mL; fermentation refers to fermentation at 37℃ for 72 hours, with the pH controlled at 6.5 during fermentation.
[0045] The mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water in step A3 is 5.1:2.1:1:0.25:0.3:11; stirring refers to stirring at 3000 rpm for 25 minutes.
[0046] The method for preparing the serum that helps activate cell activity includes the following steps: C1. Octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol are mixed and stirred in a water bath at 75°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 80°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 10,000 rpm for 6 minutes, and then cool to 45°C to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 250 rpm for 20 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5.2, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.
[0047] Example 4 An essence that helps activate cell activity, the essence comprising, by weight, 8 parts cell activity promoting composition, 7.5 parts glycerin, 6 parts butylene glycol, 2.5 parts 1,2-pentanediol, 3.2 parts octyl polymethylsiloxane, 1.6 parts polydimethylsiloxane, 1.1 parts hydrogenated lecithin, 0.065 parts tetrahydropiperine, 1.1 parts octyldodecanol, 2.3 parts trehalose, 0.35 parts tetrahydromethylpyrimidine carboxylic acid, 0.25 parts fibronectin, 0.04 parts acetyl hexapeptide-8, 0.12 parts sodium heparin, 0.6 parts allantoin, 1.3 parts panthenol, 0.4 parts sodium hyaluronate, 0.12 parts xanthan gum, 0.5 parts ethylhexylglycerin, and 68 parts deionized water.
[0048] The cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0049] The mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 1.1:0.55:2.2:0.95:0.06:0.0011.
[0050] Step A1 involves preparing the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through a 100-mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 75wt% ethanol solution, reflux at 83℃ for 2.2h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell to obtain banana fruit ethanol extract, add cellulase and pectinase, adjust the pH to 4.4, enzymatically hydrolyze at 51℃ for 3.7h, inactivate, centrifuge, filter to obtain banana fruit extract. B3. The crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree were mixed with 85wt% ethanol solution and refluxed at 83℃ for 2.2h. After repeating this process several times, the extracts were combined and concentrated under reduced pressure until there was no alcohol odor, to obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Cellulase was added to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, and the pH was adjusted to 5.1. Each extract was enzymatically hydrolyzed at 38°C for 2.8 h, inactivated, centrifuged, and filtered to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
[0051] The mass ratio of the crushed banana fruit and 75wt% ethanol solution in step B2 is 1:15.8; the mass ratio of banana fruit ethanol extract, cellulase, and pectinase is 1:0.017:0.013.
[0052] The mass ratio of the crushed Myrothamnus fruticosa to the 85wt% ethanol solution in step B3 is 1:14.6; the mass ratio of the crushed Sea Fennel to the 85wt% ethanol solution is 1:13.3; and the mass ratio of the crushed Palm Leaf Tree to the 85wt% ethanol solution is 1:15.2.
[0053] In step B4, the mass ratio of Myrothamnus flavescentis extract to cellulase is 1:0.025; the mass ratio of sea fennel extract to cellulase is 1:0.019; and the mass ratio of palm-leaf ginseng extract to cellulase is 1:0.023.
[0054] The inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 8.5 × 10⁻⁶. 8 CFU / mL; fermentation refers to fermentation at 38℃ for 74 hours, with the pH controlled at 6.6 during fermentation.
[0055] The mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water in step A3 is 5.15:2.2:1.1:0.28:0.32:11.5; stirring refers to stirring at 3500 rpm for 27 minutes.
[0056] The method for preparing the serum that helps activate cell activity includes the following steps: C1. Octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol are mixed and stirred in a water bath at 76°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 81°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 11000 rpm for 7 minutes, and then cool to 46℃ to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 300 rpm for 22 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5.3, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.
[0057] Example 5 An essence that helps activate cell activity, the essence comprising, by weight, 9 parts cell activity promoting composition, 8 parts glycerin, 7 parts butylene glycol, 3 parts 1,2-pentanediol, 4 parts octyl polymethylsiloxane, 2 parts polydimethylsiloxane, 1.2 parts hydrogenated lecithin, 0.08 parts tetrahydropiperine, 1.2 parts octyldodecanol, 2.5 parts trehalose, 0.4 parts tetrahydromethylpyrimidine carboxylic acid, 0.3 parts fibronectin, 0.05 parts acetyl hexapeptide-8, 0.15 parts sodium heparin, 0.8 parts allantoin, 1.5 parts panthenol, 0.5 parts sodium hyaluronate, 0.15 parts xanthan gum, 0.6 parts ethylhexylglycerin, and 70 parts deionized water.
[0058] The cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0059] The mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 1.2:0.6:2.3:1.1:0.06:0.0012.
[0060] Step A1 involves preparing the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through a 100-mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 76wt% ethanol solution, reflux at 83℃ for 2.5h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell to obtain banana fruit ethanol extract, add cellulase and pectinase, adjust the pH to 4.5, enzymatically hydrolyze at 52℃ for 4h, inactivate, centrifuge, filter, and obtain banana fruit extract. B3. The crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree were mixed with 86wt% ethanol solution and refluxed at 83℃ for 2.5h. After repeating this process several times, the extracts were combined and concentrated under reduced pressure until there was no alcohol odor, to obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Cellulase was added to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, and the pH was adjusted to 5.2. Each extract was enzymatically hydrolyzed at 39°C for 3 hours, inactivated, centrifuged, and filtered to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
[0061] The mass ratio of the crushed banana fruit and the 76wt% ethanol solution in step B2 is 1:16; the mass ratio of the banana fruit ethanol extract, cellulase, and pectinase is 1:0.018:0.014.
[0062] The mass ratio of the crushed Myrothamnus fruticosa and 86wt% ethanol solution in step B3 is 1:15; the mass ratio of the crushed sea fennel and 86wt% ethanol solution is 1:13.5; and the mass ratio of the crushed palm-leaf tree and 86wt% ethanol solution is 1:15.3.
[0063] In step B4, the mass ratio of Myrothamnus flavescentis extract to cellulase is 1:0.026; the mass ratio of sea fennel extract to cellulase is 1:0.02; and the mass ratio of palm-leaf extract to cellulase is 1:0.024.
[0064] The inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 9 × 10⁻⁶. 8 CFU / mL; fermentation refers to fermentation at 39℃ for 76 hours, with the pH controlled at 6.6 during fermentation.
[0065] The mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water in step A3 is 5.2:2.3:1.2:0.3:0.35:12; stirring refers to stirring at 3500 rpm for 30 minutes.
[0066] The method for preparing the serum that helps activate cell activity includes the following steps: C1. Octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol are mixed and stirred in a water bath at 77°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 82°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 12000 rpm for 7 minutes, and then cool to 46℃ to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 300 rpm for 25 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5.4, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.
[0067] Comparative Example 1 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the extracts of Myrothamnus flabellifolia, sea fennel, palm leaf tree, glucose, and yeast extract and stir until homogeneous to obtain a base solution; the mass ratio of the extracts of Myrothamnus flabellifolia, sea fennel, palm leaf tree, glucose, and yeast extract is 1.5:2.1:0.8:0.05:0.001. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0068] Comparative Example 2 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the banana fruit extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir evenly to obtain a base solution; the mass ratio of the banana fruit extract, sea fennel extract, palm leaf extract, glucose, and yeast extract is 1:2.6:0.8:0.05:0.001. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0069] Comparative Example 3 A1. Mix the banana fruit extract, myrothamnus flabellifolia extract, palm leaf extract, glucose, and yeast extract and stir evenly to obtain a base solution; the mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, palm leaf extract, glucose, and yeast extract is 1:0.5:2.9:0.05:0.001. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0070] Comparative Example 4 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, glucose, and yeast extract and stir evenly to obtain a base solution; the mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract is 1.8:0.5:2.1:0.05:0.001. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
[0071] Comparative Example 5 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Mix the base solution, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition; the mass ratio of the base solution, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water is 5.1:2.1:1:0.25:0.3:11.
[0072] Comparative Example 6 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition; the mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water is 5.1:3.1:0.25:0.3:11.
[0073] Comparative Example 7 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition; the mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, sodium hyaluronate, and deionized water is 5.35:2.1:1:0.3:11.
[0074] Comparative Example 8 Based on Example 3, while keeping other conditions unchanged, the preparation method of the cell activity promoting composition was changed to include the following steps: A1. Mix the banana fruit extract, myrothamnus extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, and deionized water, and stir to obtain a cell activity promoting composition; the mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, and deionized water is 5.4:2.1:1:0.25:11.
[0075] 1. Patch test Thirty volunteers with healthy skin were selected, half male and half female, aged between 30 and 50. All volunteers had no history of skin diseases, no known allergies to the test materials, and had not used any drugs or cosmetics that might affect skin reactions within at least two weeks before the test. Using the serums prepared in Examples 1-5 that help activate cell activity as samples, 0.02 mL of the sample was placed in a spot tester and attached to the flexor side of the subject's forearm with hypoallergenic adhesive tape. After 24 hours, the spot tester was removed, and skin reactions were observed at 0.5 h, 24 h, and 48 h after removal. The results were recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Specifications" (2015 edition). The scoring was divided into grades 0-4, with grade 0 being a negative reaction. The test results are shown in Table 1. As shown in Table 1, the serum ingredients prepared in Examples 1-5 of this invention that help activate cell activity are safe and gentle, and will not irritate the skin during use.
[0076] 2. Repair performance test Sixty-five volunteers were selected, half male and half female, aged between 30 and 50. All volunteers had no history of skin disease, no known allergies to the test materials, and had not used any drugs or cosmetics that might affect skin reaction within at least two weeks before the test. Volunteers were randomly divided into 13 groups. The amount of skin erythema was measured using a Mexameter MXI8 erythrocyte analyzer on a pre-defined test area on the forearm of each subject, and this erythema amount was set as 0 (baseline value). 0.02 mL of a 3 wt% sodium lauryl sulfate aqueous solution was placed in the center of the test chamber of the spot treatment device, and then hypoallergenic adhesive tape was applied to the pre-defined area on the forearm of each subject. After 24 hours of application, the test chamber was removed, and the amount of skin erythema was measured 4 hours later, set as 100%. Then, on days 1-3 after treatment with the sodium lauryl sulfate aqueous solution, the same amount (1 mL) of the serum prepared in Examples 1-5 and Comparative Examples 1-8) was applied to the test area on the forearm of each subject. The amount of skin erythema was measured 4 hours after application each day and recorded as shown in Table 2 below.
[0077] As shown in Table 2, the essence prepared by this invention, which helps to activate cell activity, can provide the skin with a variety of nutrients, thereby promoting cell metabolism, assisting in activating cell activity, and effectively restoring and strengthening the skin barrier, thus improving skin condition.
[0078] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.
Claims
1. An essence that helps activate cell activity, characterized in that: The ingredients of the essence, by weight, include 5-9 parts of a cell activity promoting composition, 6-8 parts of glycerin, 3-7 parts of butylene glycol, 1-3 parts of 1,2-pentanediol, 1-4 parts of octyl polymethylsiloxane, 1-2 parts of polydimethylsiloxane, 0.8-1.2 parts of hydrogenated lecithin, 0.02-0.08 parts of tetrahydropiperine, 0.8-1.2 parts of octyldodecanol, and 1.5-2.5 parts of trehalose. 0.2-0.4 parts tetrahydromethylpyrimidine carboxylic acid, 0.1-0.3 parts fibronectin, 0.01-0.05 parts acetyl hexapeptide-8, 0.05-0.15 parts sodium heparin, 0.2-0.8 parts allantoin, 0.5-1.5 parts panthenol, 0.1-0.5 parts sodium hyaluronate, 0.05-0.15 parts xanthan gum, 0.2-0.6 parts ethylhexylglycerin, and 60-70 parts deionized water.
2. The essence according to claim 1, characterized in that: The cell activity-enhancing composition is obtained by a preparation method comprising the following steps: A1. Mix the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract and stir well to obtain the base solution. A2. Inoculate Lactobacillus plantarum into the base liquid, ferment, inactivate, centrifuge, filter, and obtain fermentation broth; A3. Mix the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine peptide, sodium hyaluronate, and deionized water, and stir to obtain a cell activity promoting composition.
3. The essence according to claim 2, characterized in that: The mass ratio of the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, palm leaf extract, glucose, and yeast extract in step A1 is 0.8-1.2:0.4-0.6:1.9-2.3:0.5-1.1:0.04-0.06:0.0008-0.0012.
4. The essence according to claim 2, characterized in that: Step A1 involves preparing the banana fruit extract, myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract according to the following steps: B1. After pulverizing the dried banana fruit, myrothamnus flabellifolia, sea fennel, and palm-leaf tree, pass them through a 60-100 mesh sieve to obtain pulverized banana fruit, pulverized myrothamnus flabellifolia, pulverized sea fennel, and pulverized palm-leaf tree. B2. Mix the crushed banana fruit with 72-76wt% ethanol solution, reflux at 81-83℃ for 1.5-2.5h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol odor to obtain banana fruit ethanol extract, then add cellulase and pectinase, adjust the pH to 4.1-4.5, enzymatically hydrolyze at 48-52℃ for 3-4h, inactivate, centrifuge, filter, and obtain banana fruit extract; B3. Mix the crushed Myrothamnus flabellifolia, crushed sea fennel, and crushed palm-leaf tree with 80-86wt% ethanol solution, reflux at 81-83℃ for 1.5-2.5h, repeat several times, combine the extracts, concentrate under reduced pressure until there is no alcohol odor, and obtain Myrothamnus flabellifolia ethanol extract, sea fennel ethanol extract, and palm-leaf tree ethanol extract. B4. Add cellulase to the extracts of Myrothamnus flabellifolia, sea fennel, and palm-leaf tree, respectively, adjust the pH to 4.8-5.2, and enzymatically hydrolyze each extract at 35-39℃ for 2-3 hours. After inactivation, centrifuge and filter to obtain Myrothamnus flabellifolia extract, sea fennel extract, and palm-leaf tree extract.
5. The essence according to claim 4, characterized in that: The mass ratio of the crushed banana fruit and the 72-76 wt% ethanol solution in step B2 is 1:15-16; the mass ratio of the banana fruit ethanol extract, cellulase, and pectinase is 1:0.012-0.018:0.01-0.
014.
6. The essence according to claim 4, characterized in that: The mass ratio of the crushed Myrothamnus fruticosa and the 80-86 wt% ethanol solution in step B3 is 1:14-15; the mass ratio of the crushed Sea Fennel and the 80-86 wt% ethanol solution is 1:12.7-13.5; and the mass ratio of the crushed Palm Leaf Tree and the 80-86 wt% ethanol solution is 1:14.7-15.
3.
7. The essence according to claim 4, characterized in that: In step B4, the mass ratio of Myrothamnus flavescentis alcohol extract to cellulase is 1:0.02-0.026; the mass ratio of Amygdalina scabra alcohol extract to cellulase is 1:0.016-0.02; and the mass ratio of Palmarosa scabra alcohol extract to cellulase is 1:0.02-0.
024.
8. The essence according to claim 2, characterized in that: The inoculum size of *Lactobacillus plantarum* in the substrate solution in step A2 is 7-9 × 10⁻⁶. 8 CFU / mL; the fermentation refers to fermentation at 35-39℃ for 68-76h, with the pH controlled at 6.4-6.6 during fermentation.
9. The essence according to claim 2, characterized in that: The mass ratio of the fermentation broth, Lactobacillus fermentation lysate, Pichia pastoris fermentation lysate filtrate, arginine / lysine polypeptide, sodium hyaluronate, and deionized water in step A3 is 5-5.2:1.9-2.3:0.8-1.2:0.2-0.3:0.25-0.35:10-12; the stirring refers to stirring at 2500-3500 rpm for 20-30 minutes.
10. A method for preparing an essence that helps activate cell activity as described in any one of claims 1-9, characterized in that: The method for preparing the serum that helps activate cell activity includes the following steps: C1. Mix octyl polymethylsiloxane, polydimethylsiloxane, hydrogenated lecithin, tetrahydropiperidine, and octyldodecyl alcohol, and stir in a water bath at 73-77°C until completely dissolved to obtain the oil phase; C2. Glycerin, butanediol, 1,2-pentanediol, trehalose, tetrahydromethylpyrimidine carboxylic acid, fibronectin, sodium heparin, allantoin, panthenol, and deionized water are mixed and stirred at 78-82°C until completely dissolved to obtain an aqueous phase. C3. Add the oil phase to the water phase, stir at 8000-12000 rpm for 5-7 minutes, and then cool to 44-46℃ to obtain mixture one; C4. Add the cell activity promoting composition, acetyl hexapeptide-8, sodium hyaluronate, and ethylhexylglycerin to the mixture one, and stir at 200-300 rpm for 15-25 min to obtain the mixture two. C5. Adjust the pH of the mixture to 5-5.4, add xanthan gum, stir evenly, degas under vacuum, and aseptically fill to obtain the final product.